ライフサイエンスコーパス: 3'-5'-exonuclease

1 while proofreading errors with its intrinsic 3' 5' exonuclease.

2 ted element and which blocks the action of a 3'-5' exonuclease.

3 e domain did not function independently as a 3'-5' exonuclease.

4 a catalytically bifunctional DNA polymerase:3'-5' exonuclease.

5 A-DNA hybrid duplex is the substrate for the 3'-5' exonuclease.

6 nged to that predicted for the activity of a 3'-5' exonuclease.

7 ameshift mutations in TREX1, which encodes a 3'-5' exonuclease.

8 product and to Ustilago maydis Rec1, a known 3'->5'exonuclease.

9 at sidRNA production depends on distributive 3'-5' exonucleases.

10 r then could be subject to proofreading by a 3'-->5' exonuclease.

11 We recently showed that hWRN is also a 3'-->5' exonuclease.

12 r poly(U) to protect RNA substrates from the 3'-->5' exonuclease.

13 The TREX1 and TREX2 genes encode mammalian 3'-->5' exonucleases.

14 s that these recombinant proteins are active 3'-->5' exonucleases.

15 ecBCD enzyme, which is both a helicase and a 3' --> 5' exonuclease.

16 the RecQ DNA helicase family that contains a 3' --> 5'-exonuclease.

17 xonucleolytic processing by a combination of 3' --> 5' exonucleases.

18 to regulate histone mRNA metabolism, and the 3'-5' exonuclease 3'hExo trims its 3'-end after processi

19 Through this 3'-5' exonuclease action the total 5S RNA of the mutant

20 This fragment contains both the 3'-5' exonuclease active site and part of the autologous

21 l gamma) shares conserved DNA polymerase and 3'-5' exonuclease active site motifs with Escherichia co

22 There are two main differences between the 3'-5' exonuclease active site regions of the two protein

23 fractional occupancies of the polymerase and 3'-5' exonuclease active sites of Klenow fragment.

24 A primer terminus between the polymerase and 3'-5' exonuclease active sites of the mutant proteins wa

25 To discern how the polymerase and 3' --> 5' exonuclease activities contribute to the high

26 We find that both the polymerization and 3' --> 5' exonuclease activities of gp43 are totally inh

27 tical properties, and the DNA polymerase and 3' --> 5' exonuclease activities were shown unambiguousl

28 n that possesses both 3' --> 5' helicase and 3' --> 5' exonuclease activities.

29 es were used to test whether 5' -->3' and/or 3' -->5' exonuclease activities mediated degradation.

30 nation of mismatch-specific endonuclease and 3'-->5' exonuclease activities in the reaction mixture.

31 n2 protein contains 3'-phosphodiesterase and 3'-->5' exonuclease activities, and mutation of the acti

32 ince none of these processing steps involves 3'-->5' exonuclease activities, the requirement for the

33 ease activities as well as deadenylating and 3'-->5' exonuclease activities.

34 ase family and contains 3'-->5' helicase and 3'-->5' exonuclease activities.

35 protein (WRN) that has ATPase, helicase and 3'-->5' exonuclease activities.

36 ed polypeptide has both 3'-->5' helicase and 3'-->5' exonuclease activities.

37 dividual contributions of its polymerase and 3'-->5' exonuclease activities.

38 es catalytic centers for both polymerase and 3'-5' exonuclease activities that are separated by about

39 t DNA polymerases with a range of attenuated 3'-5' exonuclease activities was constructed from a chim

40 t (PolD1) possessing both DNA polymerase and 3'-5' exonuclease activities, and a smaller subunit (Pol

41 subunit, which has intrinsic polymerase and 3'-5' exonuclease activities, contains sequence motifs t

42 it contains both RNA and single-stranded DNA 3'-5' exonuclease activities.

43 e family, PolB possesses both polymerase and 3'-5' exonuclease activities.

44 purified protein had both DNA polymerase and 3'-5' exonuclease activities.

45 parent mass) and has both DNA polymerase and 3'-5'-exonuclease activities.

46 (in the context of a single-nucleotide gap), 3' --> 5' exonuclease activity (in the context of a nick

47 the fast mismatch excision catalyzed by the 3' --> 5' exonuclease activity further lowers the error

48 This represents the first demonstration of 3' --> 5' exonuclease activity in the polymerase catalyt

49 was completely stalled by the lesion, as its 3' --> 5' exonuclease activity increased significantly a

50 gnition of a properly oriented chi site, the 3' --> 5' exonuclease activity is attenuated.

51 Pol epsilon lacking 3' --> 5' exonuclease activity is less accurate to a deg

52 The 3' --> 5' exonuclease activity is shown to be highly dep

53 Furthermore, the 3' --> 5' exonuclease activity of DNA polymerase epsilon

54 R studies confirmed that the DNA-binding and 3' --> 5' exonuclease activity of human NDK1 is an intri

55 Finally, the 3' --> 5' exonuclease activity of PolB1 was the highest

56 te-bearing terminal nucleotide by way of the 3' --> 5' exonuclease activity of polymerase I.

57 The 3' --> 5' exonuclease activity on double-stranded DNA is

58 Replicative DNA polymerases possess 3' --> 5' exonuclease activity to reduce misincorporatio

59 The role of photoproduct structure, 3' --> 5' exonuclease activity, and processivity on poly

60 show that in addition to attenuation of the 3' --> 5' exonuclease activity, recognition of chi by th

61 find that human NDK5, NDK7, and NDK8 contain 3' --> 5' exonuclease activity.

62 man NDK1 (NM23-H1) has been reported to have 3' --> 5' exonuclease activity.

63 xamined on Klenow fragments with and without 3' --> 5' exonuclease activity.

64 Pol B100 retains a wild-type level of 3' --> 5' exonuclease activity.

65 is intermediate product is then trimmed by a 3' -->5' exonuclease activity.

66 AMHD1 protein also possesses metal-dependent 3'-->5' exonuclease activity against single-stranded DNA

67 AP endonuclease 1, which possesses 3'-->5' exonuclease activity and potentially serves as a

68 Inhibition of 3'-->5' exonuclease activity by guanosine monophosphate

69 The TREX enzymes process DNA as the major 3'-->5' exonuclease activity in mammalian cells.

70 These results demonstrate that 3'-->5' exonuclease activity is an important prerequisit

71 e only biological function attributed to the 3'-->5' exonuclease activity of DNA polymerases was proo

72 The 3'-->5' exonuclease activity of hPol further enhances po

73 Quantitative analyses revealed that the 3'-->5' exonuclease activity of p53 effectively removed

74 The 3'-->5' exonuclease activity of p53 has recently been re

75 genesis in yeast strains with defects in the 3'-->5' exonuclease activity of replicative DNA polymera

76 ny replicative polymerases have an efficient 3'-->5' exonuclease activity that excises misincorporate

77 protect otherwise unstable transcripts from 3'-->5' exonuclease activity, a phenomenon that may occu

78 nsistent with this result, in the absence of 3'-->5' exonuclease activity, both primers were extended

79 tains both a 3'-->5' helicase activity and a 3'-->5' exonuclease activity, the stimulating activity w

80 Yet the mutants retain robust 3'-->5' exonuclease activity.

81 ssays, both long isoforms were shown to have 3'-->5' exonuclease activity.

82 ML cells treated with STI-571 show increased 3'-->5' exonuclease activity.

83 ers of the recQ family, it contains a unique 3'-->5' exonuclease activity.

84 NA with 3'-->5' polarity, and also possesses 3'-->5' exonuclease activity.

85 polymerase (pol) alpha, an enzyme that lacks 3'-->5' exonuclease activity.

86 utD5 holoenzyme to have a 30-50-fold reduced 3'-->5'-exonuclease activity.

87 activity, since reverse transcriptases lack 3'-5' exonuclease activity and generally have low fideli

88 se 1 (APE1) prevented TNR expansions via its 3'-5' exonuclease activity and stimulatory effect on DNA

89 (hWRN-N(70-240)), exhibits the same level of 3'-5' exonuclease activity as the previously described e

90 rdinating with flap endonuclease 1, the APE1 3'-5' exonuclease activity cleaves the annealed upstream

91 Spo11--but a specific function for the Mre11 3'-5' exonuclease activity has remained elusive.

92 s that are homologous to those important for 3'-5' exonuclease activity in other polymerases.

93 ests that POLG1 requires both polymerase and 3'-5' exonuclease activity in the same molecule.

94 tase domain of OsCCR4a and OsCCR4b exhibited 3'-5' exonuclease activity in vitro, and point mutation

95 CAF1B, rOsCAF1G, and rOsCAF1H, all exhibited 3'-5' exonuclease activity in vitro.

96 APE2 3'-phosphodiesterase and 3'-5' exonuclease activity is essential for single-stran

97 ded strand displacement synthesis unless its 3'-5' exonuclease activity is removed.

98 However, inactivation of the 3'-5' exonuclease activity is sufficient to allow the po

99 HmtSSB enhances intrinsic 3'-5' exonuclease activity of p53, particularly in hydro

100 rporated nucleotides are excised through the 3'-5' exonuclease activity of the DNA polymerase holoenz

101 The mechanism of the 3'-5' exonuclease activity of the Klenow fragment of DNA

102 were constructed in an attempt to reduce the 3'-5' exonuclease activity of Thermococcus sp. 9 degrees

103 Abolishment of the 3'-5' exonuclease activity of wild-type pol I increased

104 DSBs stimulate the phosphorylation and 3'-5' exonuclease activity of X-Mre11 complex.

105 However, the 3'-5' exonuclease activity on polynucleotide substrates

106 and within single-stranded DNA, as well as a 3'-5' exonuclease activity on single-stranded DNA.

107 olymerase is often used either alone for its 3'-5' exonuclease activity or together with a 5'-3' exon

108 as evidenced by the significant reduction in 3'-5' exonuclease activity resulting from a Lys(12) to g

109 Many DNA polymerases also possess a separate 3'-5' exonuclease activity that is used to remove misinc

110 irected synthesis of DNA and uses a separate 3'-5' exonuclease activity to edit misincorporated bases

111 The 3'-5' exonuclease activity was assigned to NM23-H1 by vi

112 Single-strand- and double-strand-dependent 3'-5' exonuclease activity was detected, as was a margin

113 UVC-induced (254 nm) DNA damage, whereas its 3'-5' exonuclease activity was dominant in the suppressi

114 change the polymerase activity; however, the 3'-5' exonuclease activity was reduced 2-29-fold, depend

115 We now show that inhibition of MRE11 3'-5' exonuclease activity with Mirin reduces the freque

116 ds DNA replication, mediated by an intrinsic 3'-5' exonuclease activity within its PHP domain.

117 ase with efficient reverse transcriptase and 3'-5' exonuclease activity, a family of mutant DNA polym

118 p53 present in the protein complex exhibited 3'-5' exonuclease activity, it was incapable of excising

119 ount of an Archaea DNA polymerase possessing 3'-5' exonuclease activity, since reverse transcriptases

120 ect the inhibitor from excision by the viral 3'-5' exonuclease activity.

121 AN34 also displays enhanced 3'-5' exonuclease activity.

122 nt, leading to their eventual removal by the 3'-5' exonuclease activity.

123 eplicative DNA polymerase with an associated 3'-5' exonuclease activity.

124 fied enzyme demonstrated both polymerase and 3'-5' exonuclease activity.

125 kinetics, strand displacement synthesis and 3'-5' exonuclease activity.

126 lso possesses single-stranded DNA and/or RNA 3'-5' exonuclease activity.

127 OB binds to single-stranded DNA and exhibits 3'-5' exonuclease activity.

128 ng recombinant protein we confirmed that the 3'-5'-exonuclease activity of Pol delta can efficiently

129 n-terminated 3'-end of the DNA primer by the 3'-5'-exonuclease activity of Pol gamma were similar (0.

130 the ability of Pol delta to back up via its 3'-5'-exonuclease activity, a process called idling, mai

131 d RNA editing exonuclease 1 (REX1), exhibits 3'-5'-exonuclease activity.

132 Together, the 3'->5' exonuclease activity and the variable mismatch ex

133 C and the three small subunits regulates the 3'->5' exonuclease activity of the hPolepsilon holoenzym

134 Surprisingly, the 3'->5' exonuclease activity of the hPolepsilon holoenzym

135 e that the three biological functions of the 3'-->5' exonuclease addressed in this study are performe

136 t catalyzes three DNA-dependent reactions: a 3'-5'-exonuclease, an ATPase, and a 3'-5'-helicase.

137 y deficiency of the WRN protein that harbors 3' -->5' exonuclease and RecQ-type 3' --> 5' helicase ac

138 codes a multifunctional nuclear protein with 3'-->5' exonuclease and 3'-->5' helicase activities.

139 Importantly, PCNA stimulates the 3'-->5' exonuclease and 3'-phosphodiesterase activities

140 protein of Saccharomyces cerevisiae contains 3'-->5' exonuclease and 3'-phosphodiesterase activities,

141 ination hot spot chi, RecBCD enzyme has both 3'-->5' exonuclease and a weaker 5'-->3' exonuclease act

142 s preferentially on single-stranded DNA as a 3'-->5' exonuclease and also functions as a 3'-phosphodi

143 We therefore examined WRN, a 3'-->5' exonuclease and helicase mutated in Werner syndr

144 odes a nuclear protein (WRN) which possesses 3'-5' exonuclease and ATPase-dependent 3'-5' helicase ac

145 es sequential detection of the activities of 3'-5' exonuclease and DNase I in cell lysates.

146 o of its attendant enzymatic activities, the 3'-5' exonuclease and nucleoside diphosphate kinase, are

147 eplication by ISG20 and PKR depends on their 3'-5' exonuclease and protein kinase activities, respect

148 by the Escherichia coli Klenow fragment (KF) 3'-5' exonuclease and snake venom phosphodiesterase.

149 imers is possible only by cooperation of the 3'-5'-exonuclease and DNA polymerase activities of the e

150 endence of Escherichia coli exonuclease III, 3'-5'-exonuclease and exoribonuclease H activities is re

151 only) and recJ (5'-->3' exonuclease), xonA (3'-->5' exonuclease) and partially dependent on recQ (he

152 a subunit (polymerase), the epsilon subunit (3'-->5' exonuclease), and the theta subunit, in the line

153 ture is folded into NH(2)- terminal, editing 3'-5' exonuclease, and polymerase domains that are topol

154 rotein complex composed of RET1 TUTase, DSS1 3'-5' exonuclease, and three additional subunits.

155 formamido-pyrimidine-DNA glycosylase (FPG), 3'-5' exonucleases, and enzymes with template-independen

156 Related exosome complexes of 3'-->5' exonucleases are present in the nucleus and the

157 3'-5' Exonucleases are believed to play an important rol

158 re, we identified PNLDC1, an uncharacterized 3'-5' exonuclease, as Trimmer in silkworms.

159 This enzyme was originally identified as a 3' --> 5' exonuclease, but we show here that NrnA is bid

160 We have defined NM23-H1 biochemically as a 3'-5' exonuclease by virtue of its ability in stoichiome

161 beta long noncoding RNAs are protected from 3'-5' exonucleases by highly conserved triple helical st

162 s is required before RNase P and the various 3' --> 5' exonucleases can complete tRNA maturation.

163 lian TREX1 and TREX2 proteins contain potent 3'-->5' exonucleases capable of functioning in this capa

164 The 3'-->5' exonucleases catalyze the excision of nucleoside

165 Kinetic parameters for 3'-5' exonuclease cleavage of single- and double-strande

166 y impaired exonuclease activity and that the 3'-5' exonuclease contributes substantially to the fidel

167 suggest the existence of a highly conserved 3'-5' exonuclease core domain within Rrp6p.

168 sing exonuclease III and T4 DNA polymerase's 3'-->5' exonuclease, covers approximately 35 base pairs

169 Nase I, lambda exonuclease and T4 polymerase 3'-->5'exonuclease, covers approximately 40 nt and is ne

170 ine leukemia virus reverse transcriptase and 3' --> 5' exonuclease-deficient (exo-) Vent polymerase w

171 However, it has been shown that a 3'-->5' exonuclease-deficient form of the Klenow fragmen

172 olymerases Sequenase 1.0, Sequenase 2.0, and 3'-->5' exonuclease-deficient Klenow fragment greatly de

173 Primer extension studies, catalyzed by the 3'-->5' exonuclease-deficient Klenow fragment of Escheri

174 igonucleotides by both primer extension with 3'-->5'exonuclease-deficient Klenow fragment or T4 polym

175 template-directed nucleotide addition by the 3'-5' exonuclease-deficient large fragment of Escherichi

176 substitutions in the polymerase domain of a 3'-5'-exonuclease-deficient Klenow fragment.

177 OLE1 transcript acts by activating exosomal 3' --> 5'-exonuclease degradation activity.

178 s greatly elevated in phage deficient in the 3'-5' exonuclease, DexA, suggesting that the length of 3

179 the role of the Perlman syndrome-associated 3'-5' exonuclease Dis3l2 in rRNA processing.

180 PCR assays, we show here that the bacterial 3'-5' exonucleases DnaQ and ExoT can trim long 3' overha

181 Our findings implicate the E. coli host 3'-5' exonucleases DnaQ and ExoT in spacer adaptation an

182 even a heterozygous pol3-01 mutation in the 3'-->5' exonuclease domain of DNA polymerase delta.

183 main homologous to bacterial RNase D and the 3'-5' exonuclease domain of DNA polymerase I in the Wern

184 The editing 3'-5' exonuclease domain of gp43 is homologous to that o

185 rtitioning of DNA to the spatially separated 3'-5' exonuclease domain, providing an additional mechan

186 EXD2 (3'-5' exonuclease domain-containing protein 2) is an ess

187 l protein with a conserved DEDDy superfamily 3'-5' exonuclease domain.

188 ion of the WS protein containing a potential 3'-5' exonuclease domain.

189 The D368A mutation within the 3'-5'-exonuclease domain of the herpes simplex type 1 DN

190 rone, owing to their lack of a proofreading (3'- 5' exonuclease) domain.

191 n each of the three DNA polymerase and three 3' --> 5' exonuclease domains identified by biochemical

192 chondrial degradosome, which also includes a 3' --> 5' exonuclease, Dss1p.

193 polymerase subunit, alpha, the proofreading 3'-->5' exonuclease, epsilon, and a subunit of unknown f

194 (pol) site, DNA polymerases have a separate 3' --> 5' exonuclease (exo) editing activity that is inv

195 Many DNA polymerases (Pol) have an intrinsic 3'-->5' exonuclease (Exo) activity which corrects polyme

196 mulate the polymerase (pol) activity and the 3'-5' exonuclease (exo) activity of T4 DNA polymerase (4

197 of a replicase generally have a more potent 3'-5' exonuclease (exo) activity than A family DNA polym

198 ent failures, result in substitutions in the 3'-5' exonuclease (Exo) domain of the catalytic subunit

199 d by both the 5'-3' exonuclease RecJ and the 3'-5' exonuclease ExoI, observations that suggest the im

200 at of other RNA viruses and is mediated by a 3'-->5' exonuclease (ExoN) activity that probably functi

201 PAP I competes with the 3' -> 5' exonucleases for pre-tRNA substrates adding sho

202 When the 3'-->5' exonuclease free (exo-) Klenow fragment of DNA p

203 primer extension reactions catalyzed by the 3' --> 5' exonuclease-free (exo(-)) Klenow fragment of E

204 primer extension reactions catalyzed by the 3'-->5' exonuclease-free Klenow fragment of Escherichia

205 insight into the mechanism for Nfo-catalyzed 3'-->5' exonuclease function and its inhibition by 3'-te

206 D-loop structure is readily attacked by the 3'-->5' exonuclease function of WRN.

207 Unlike gamma DNA polymerases, ablation of 3'-5' exonuclease function resulted in a modest 5-8-fold

208 es 3'-phosphodiesterase, 3'-phosphatase, and 3'-->5'-exonuclease functions specific for the 3' termin

209 y I-RNase is non-specific; I-RNase acts as a 3'-->5' exonuclease generating 5'-NMPs as products.

210 Here we identify oligoribonuclease (Orn)-a 3'-->5' exonuclease highly conserved among Actinobacteri

211 te internucleotide linkages are resistant to 3'-->5' exonuclease hydrolysis, rendering the target DNA

212 d-type 9 degrees N polD were used to examine 3'-5' exonuclease hydrolysis activity in the presence of

213 a cell death process implicating this major 3' --> 5' exonuclease in genomic DNA degradation to mini

214 Both the 5' --> 3' DNA polymerase and 3' --> 5' exonuclease in pol gamma are stimulated 15-20-

215 monstrated both 5' --> 3' DNA polymerase and 3' --> 5' exonuclease in the recombinant polypeptide.

216 (U) tracts protected RNA substrates from the 3'-->5' exonuclease in a protein-dependent fashion.

217 il blocks the activity of a highly efficient 3'-->5' exonuclease in HeLa extracts.

218 e to dnaQ, encoding the epsilon proofreading 3'-5'-exonuclease, interacts with alpha but does not for

219 hereby RNase T and RNase PH, the two primary 3' --> 5' exonucleases involved in the final step of 3'-

220 ither RNase T and/or RNase PH, the two major 3' --> 5' exonucleases involved in the final step of tRN

221 The yeast exosome is a complex of 3'-->5' exonucleases involved in RNA processing and degr

222 Subsequently, a group of 3' -> 5' exonucleases mature the 3' ends of the majority

223 e to natural substrates, indicating that the 3' --> 5' exonuclease may contribute to DNA synthesis in

224 Six substitutions in the 3'-5' exonuclease motif I were constructed in an attempt

225 of more than 100 bases per second and has a 3'-->5' exonuclease (nucleotide removing) activity at a

226 erminus unstacking, and base excision by the 3' --> 5' exonuclease of bacteriophage T4 (T4 pol) was e

227 We show that this mutation inactivates the 3' --> 5' exonuclease of poldelta and causes a mutator a

228 trosourea (MNU)-treated DNA templates by the 3' --> 5' exonuclease of T4 DNA polymerase.

229 lso overcomes this inhibition to promote the 3' -> 5' exonuclease of MRX, which requires ATP hydrolys

230 These results suggest a possibility that the 3'-->5' exonuclease of the wt p53 protein might provide

231 d on genetic and biochemical analysis of the 3'-->5' exonuclease of yeast DNA polymerase delta (Pol d

232 it mechanistic requirement for the intrinsic 3'-5'-exonuclease of pol delta.

233 In Escherichia coli, the effects of several 3'-5' exonucleases on RecA loading were studied by assay

234 pically contained a DNA polymerase devoid of 3'-5' exonuclease, or "proofreading", activity blended w

235 The exosome complex of 3'-5' exonucleases participates in RNA maturation and qu

236 lease activity of T4 DNA polymerase (43Exo), 3'-5' exonucleases, play a role in intron homing.

237 The 3' --> 5'-exonucleases process DNA ends in many DNA repa

238 Furthermore, ExoI, ExoIII and the other 3'-5' exonucleases process these DSBs, antagonizing the

239 Zf-GRF mutations impact APE2 DNA binding and 3'-5' exonuclease processing, and also prevent efficient

240 erted ribonucleotides escape proofreading by 3' --> 5' exonuclease-proficient Pol epsilon, indicating

241 yeast Saccharomyces cerevisiae that possess 3' --> 5' exonuclease proofreading activity.

242 ur in the absence of the Umu proteins if the 3'-5' exonuclease proofreading activity of the pol III e

243 family of proteins that have homology to the 3'-5' exonuclease proofreading subunit (DnaQ) of E. coli

244 The 3'-5' exonuclease rate was measured at 0.18 s-1.

245 ing that the N420 side chain facilitates the 3'-5' exonuclease reaction by introducing strain into th

246 RNA polymerases based on the analysis of its 3'-5' exonuclease reaction in the context of crystal str

247 kpoint protein Rad17 and the Ustilago maydis 3' --> 5' exonuclease, Rec1.

248 ation and characterization of a nonexosomal, 3'-->5' exonuclease required for SL RNA 3'-end formation

249 Here we identify Dis3l2 as the 3'-5' exonuclease responsible for the decay of uridylate

250 Here, we show that the 3'-->5' exonuclease RNase II plays an important role in

251 has become standard in the field to use the 3'-5' exonuclease RNase R.

252 pathway that does not involve any of the six 3' --> 5' exonucleases (RNase T, RNase PH, RNase D, RNas

253 sites, a DNA polymerization site (pol) and a 3'-5' exonuclease site (exo) for proofreading.

254 partitioning of the DNA substrates into the 3'-5' exonuclease site by 3-7-fold, relative to the perf

255 ed the level of partitioning of DNA into the 3'-5' exonuclease site by 8- and 18-fold, respectively,

256 ases to promote partitioning of DNA into the 3'-5' exonuclease site decreased in the following order:

257 er 3' terminus in the polymerase site or the 3'-5' exonuclease site of the enzyme, respectively.

258 o the binding of DNA primer-templates to the 3'-5' exonuclease site of the large proteolytic fragment

259 binding of mismatched DNA substrates to the 3'-5' exonuclease site relative to DNA bound at the poly

260 partitioning of bulged DNA substrates to the 3'-5' exonuclease site relative to that of properly base

261 ands to two divalent metal ions bound at the 3'-5' exonuclease site, designated A and B, indicated th

262 d a surprising preference for binding at the 3'-5' exonuclease site, despite the absence of mismatche

263 trates increased their partitioning into the 3'-5' exonuclease site, in accord with the results of an

264 promotes transfer of a DNA substrate to the 3'-5' exonuclease site, suggesting that the premutationa

265 the partitioning of mismatched DNA into the 3'-5' exonuclease site, suggesting that the tyrosine sid

266 , yielding a polymerizing complex, or to the 3'-5' exonuclease site, yielding an editing complex.

267 phically defined DNA-protein contacts at the 3'-5' exonuclease site.

268 proximity to a DNA 3' terminus bound at the 3'-5' exonuclease site.

269 d that metal A also helps to bind DNA to the 3'-5' exonuclease site.

270 ects on the binding of mismatched DNA to the 3'-5' exonuclease site.

271 primer/template to the 5'-3' polymerase and 3'-5' exonuclease sites of KF.

272 fractional occupancies of the polymerase and 3'-5' exonuclease sites of the enzyme for DNA substrates

273 a distribution of DNA between polymerase and 3'-5' exonuclease sites that was similar to that observe

274 e and revealed its association with Rrp6p, a 3'-->5' exonuclease specific to the nuclear exosome frac

275 TREX2 is an autonomous nonprocessive 3' --> 5' exonuclease, suggesting that it maintains geno

276 Furthermore, TREX2 (a 3'-->5' exonuclease) suppressed identical repeat fusion

277 by approximately threefold and was the only 3'-5' exonuclease tested that did not deleteriously affe

278 TREX1 is a potent 3' --> 5' exonuclease that degrades single- and double-s

279 Trex2 is a 3' --> 5' exonuclease that removes 3'-mismatched sequenc

280 Mre11 from Saccharomyces cerevisiae is a 3' -> 5' exonuclease that is responsible for 5' end degr

281 w here, in vitro and in vivo, that TdTL is a 3'-->5' exonuclease that catalyzes the deletion of nucle

282 uplication of the bacterial chromosome, is a 3'-->5' exonuclease that functions as a proofreader for

283 was isolated as a major nuclear DNA-specific 3'-->5' exonuclease that is widely distributed in both p

284 terfere with replication, whereas TREX2 is a 3'-->5' exonuclease that removes 3' mismatched nucleotid

285 is thaliana AtRrp4p is shown to be an active 3'-->5' exonuclease that requires a free 3'-hydroxyl and

286 inant holoenzyme contains DNA polymerase and 3'-5' exonuclease that are stimulated substantially by b

287 ty DNA replication depends on a proofreading 3'-5' exonuclease that is associated with the replicativ

288 We showed that these are U-specific 3'-5'-exonucleases that are inhibited by base pairing of

289 ut distinct forms of a complex containing 10 3'-->5' exonucleases, the exosome, are found in yeast nu

290 el to explain RecBCD's transformation from a 3' --> 5' exonuclease to a helicase when it meets a chi

291 ically increased the ability of the enzyme's 3'-->5' exonuclease to remove mispaired 3' bases in a pr

292 h mutations identified in genes encoding the 3'-->5' exonuclease TREX1 and the three subunits of the

293 these DNA fragments and that the cytoplasmic 3'-5' exonuclease Trex1 is required for their degradatio

294 e identified the cDNA sequences encoding two 3'-->5' exonucleases (TREX1 and TREX2) from mammalian ce

295 The size of fragments protected from 3'-->5' exonuclease trimming increases with increasing i

296 ral autonomous and DNA polymerase-associated 3'-5' exonucleases using a lacZ forward mutation assay.

297 otifs of B (alpha-)-type DNA polymerases and 3'-5' exonuclease were identified in the 845-amino-acid

298 A number of 3'-5' exonucleases were found to lower the error rate of

299 her show that the mouse WRN (mWRN) is also a 3'-->5' exonuclease, with substrate specificity similar

300 eas the combination of I709F and lack of the 3'-5' exonuclease yielded a 400-fold increase.