ライフサイエンスコーパス: 5'-exonuclease

1 ins (PCBPs) to protect uncapped PV mRNA from 5' exonuclease.

2 er CPSF-73 functions as an endonuclease or a 5' exonuclease.

3 shift mutations in TREX1, which encodes a 3'-5' exonuclease.

4 element and which blocks the action of a 3'-5' exonuclease.

5 omain did not function independently as a 3'-5' exonuclease.

6 ransferase, and primase, and is also a 3' to 5' exonuclease.

7 le proofreading errors with its intrinsic 3' 5' exonuclease.

8 the parasite extracts functioned as a 3' to 5' exonuclease.

9 n could be subject to proofreading by a 3'-->5' exonuclease.

10 We recently showed that hWRN is also a 3'-->5' exonuclease.

11 d to that predicted for the activity of a 3'-5' exonuclease.

12 Q DNA helicase family that contains a 3' --> 5'-exonuclease.

13 olytic processing by a combination of 3' --> 5' exonucleases.

14 TREX1 and TREX2 genes encode mammalian 3'-->5' exonucleases.

15 y factor in their rapid degradation by 3' to 5' exonucleases.

16 created yeast strains lacking specific 3' to 5' exonucleases.

17 t these recombinant proteins are active 3'-->5' exonucleases.

18 sidRNA production depends on distributive 3'-5' exonucleases.

19 regulate histone mRNA metabolism, and the 3'-5' exonuclease 3'hExo trims its 3'-end after processing.

20 ultiple enzymatic activities including 3' to 5' exonuclease, 3' to 5' helicase, and ssDNA annealing.

21 g DNA molecules by the concerted action of a 5' exonuclease, a DNA polymerase and a DNA ligase.

22 Biochemically, the WRN helicase and 3' to 5' exonuclease act simultaneously and cooperate to relea

23 Through this 3'-5' exonuclease action the total 5S RNA of the mutant pos

24 amma) shares conserved DNA polymerase and 3'-5' exonuclease active site motifs with Escherichia coli

25 rimer terminus between the polymerase and 3'-5' exonuclease active sites of the mutant proteins was a

26 To discern how the polymerase and 3' --> 5' exonuclease activities contribute to the high fidelit

27 n of mismatch-specific endonuclease and 3'-->5' exonuclease activities in the reaction mixture.

28 possessed very low DNA polymerase and 3' to 5' exonuclease activities in vitro.

29 e used to test whether 5' -->3' and/or 3' -->5' exonuclease activities mediated degradation.

30 find that both the polymerization and 3' --> 5' exonuclease activities of gp43 are totally inhibited

31 to inhibit both the DNA synthesis and 3' to 5' exonuclease activities of polymerases delta and epsil

32 catalytic centers for both polymerase and 3'-5' exonuclease activities that are separated by about 35

33 NA polymerases with a range of attenuated 3'-5' exonuclease activities was constructed from a chimeri

34 otein contains 3'-phosphodiesterase and 3'-->5' exonuclease activities, and mutation of the active-si

35 none of these processing steps involves 3'-->5' exonuclease activities, the requirement for the exoso

36 activities as well as deadenylating and 3'-->5' exonuclease activities.

37 amily and contains 3'-->5' helicase and 3'-->5' exonuclease activities.

38 contains both RNA and single-stranded DNA 3'-5' exonuclease activities.

39 possesses both 3' --> 5' helicase and 3' --> 5' exonuclease activities.

40 ein (WRN) that has ATPase, helicase and 3'-->5' exonuclease activities.

41 lypeptide has both 3'-->5' helicase and 3'-->5' exonuclease activities.

42 ual contributions of its polymerase and 3'-->5' exonuclease activities.

43 context of a single-nucleotide gap), 3' --> 5' exonuclease activity (in the context of a nick), and

44 protein also possesses metal-dependent 3'-->5' exonuclease activity against single-stranded DNAs and

45 tivity, since reverse transcriptases lack 3'-5' exonuclease activity and generally have low fidelity.

46 AP endonuclease 1, which possesses 3'-->5' exonuclease activity and potentially serves as a proo

47 1 (APE1) prevented TNR expansions via its 3'-5' exonuclease activity and stimulatory effect on DNA li

48 Together, the 3'->5' exonuclease activity and the variable mismatch extens

49 RN-N(70-240)), exhibits the same level of 3'-5' exonuclease activity as the previously described exon

50 nating with flap endonuclease 1, the APE1 3'-5' exonuclease activity cleaves the annealed upstream 3'

51 t the smaller human protein exhibits a 3' to 5' exonuclease activity for small (primarily </=5 nucleo

52 st mismatch excision catalyzed by the 3' --> 5' exonuclease activity further lowers the error frequen

53 11--but a specific function for the Mre11 3'-5' exonuclease activity has remained elusive.

54 e TREX enzymes process DNA as the major 3'-->5' exonuclease activity in mammalian cells.

55 s that POLG1 requires both polymerase and 3'-5' exonuclease activity in the same molecule.

56 e domain of OsCCR4a and OsCCR4b exhibited 3'-5' exonuclease activity in vitro, and point mutation of

57 1B, rOsCAF1G, and rOsCAF1H, all exhibited 3'-5' exonuclease activity in vitro.

58 pletely stalled by the lesion, as its 3' --> 5' exonuclease activity increased significantly and outc

59 hird of the wild-type enzyme and has a 3'-to-5' exonuclease activity indistinguishable from that of w

60 These results demonstrate that 3'-->5' exonuclease activity is an important prerequisite for

61 APE2 3'-phosphodiesterase and 3'-5' exonuclease activity is essential for single-stranded

62 Pol epsilon lacking 3' --> 5' exonuclease activity is less accurate to a degree sug

63 The U7-dependent 5' exonuclease activity is processive and continues degr

64 strand displacement synthesis unless its 3'-5' exonuclease activity is removed.

65 The 3' --> 5' exonuclease activity is shown to be highly dependent

66 However, inactivation of the 3'-5' exonuclease activity is sufficient to allow the polym

67 Recently, the comparatively "minor" 3' to 5' exonuclease activity of Ape1 was found to contribute

68 Furthermore, the 3' --> 5' exonuclease activity of DNA polymerase epsilon or wil

69 y biological function attributed to the 3'-->5' exonuclease activity of DNA polymerases was proofread

70 The 3'-->5' exonuclease activity of hPol further enhances polymer

71 es confirmed that the DNA-binding and 3' --> 5' exonuclease activity of human NDK1 is an intrinsic ac

72 HmtSSB enhances intrinsic 3'-5' exonuclease activity of p53, particularly in hydrolys

73 Finally, the 3' --> 5' exonuclease activity of PolB1 was the highest when 8-

74 ing terminal nucleotide by way of the 3' --> 5' exonuclease activity of polymerase I.

75 is in yeast strains with defects in the 3'-->5' exonuclease activity of replicative DNA polymerases d

76 he involvement of the recently characterized 5' exonuclease activity of RNase J1 and endonuclease act

77 the downstream products were degraded by the 5' exonuclease activity of RNase J1.

78 rated nucleotides are excised through the 3'-5' exonuclease activity of the DNA polymerase holoenzyme

79 oordinately to protect the viral genome from 5' exonuclease activity of the host mRNA decay machinery

80 Surprisingly, the 3'->5' exonuclease activity of the hPolepsilon holoenzyme wa

81 d the three small subunits regulates the 3'->5' exonuclease activity of the hPolepsilon holoenzyme.

82 The mechanism of the 3'-5' exonuclease activity of the Klenow fragment of DNA po

83 Abolishment of the 3'-5' exonuclease activity of wild-type pol I increased mut

84 DSBs stimulate the phosphorylation and 3'-5' exonuclease activity of X-Mre11 complex.

85 However, the 3'-5' exonuclease activity on polynucleotide substrates is

86 They exhibit 5' exonuclease activity on single-stranded DNA, hydrolyz

87 within single-stranded DNA, as well as a 3'-5' exonuclease activity on single-stranded DNA.

88 merase is often used either alone for its 3'-5' exonuclease activity or together with a 5'-3' exonucl

89 evidenced by the significant reduction in 3'-5' exonuclease activity resulting from a Lys(12) to glut

90 plicative polymerases have an efficient 3'-->5' exonuclease activity that excises misincorporated nuc

91 y DNA polymerases also possess a separate 3'-5' exonuclease activity that is used to remove misincorp

92 Mre11 has a 3' to 5' exonuclease activity that results in the release of m

93 cted synthesis of DNA and uses a separate 3'-5' exonuclease activity to edit misincorporated bases.

94 Replicative DNA polymerases possess 3' --> 5' exonuclease activity to reduce misincorporation of in

95 The 3'-5' exonuclease activity was assigned to NM23-H1 by virtu

96 -induced (254 nm) DNA damage, whereas its 3'-5' exonuclease activity was dominant in the suppression

97 When T4 DNA polymerase deficient in 3' to 5' exonuclease activity was employed, electron microscop

98 nge the polymerase activity; however, the 3'-5' exonuclease activity was reduced 2-29-fold, depending

99 We now show that inhibition of MRE11 3'-5' exonuclease activity with Mirin reduces the frequency

100 DNA replication, mediated by an intrinsic 3'-5' exonuclease activity within its PHP domain.

101 with efficient reverse transcriptase and 3'-5' exonuclease activity, a family of mutant DNA polymera

102 ect otherwise unstable transcripts from 3'-->5' exonuclease activity, a phenomenon that may occur nat

103 Initial characterization revealed a 3' to 5' exonuclease activity, and showed additional functiona

104 ndonuclease activity toward 5' flaps and has 5' exonuclease activity, and these activities are mediat

105 present in the protein complex exhibited 3'-5' exonuclease activity, it was incapable of excising th

106 ngle-strand DNA-binding and processive 3'-to-5' exonuclease activity, respectively.

107 t of an Archaea DNA polymerase possessing 3'-5' exonuclease activity, since reverse transcriptases la

108 both a 3'-->5' helicase activity and a 3'-->5' exonuclease activity, the stimulating activity was fo

109 the inhibitor from excision by the viral 3'-5' exonuclease activity.

110 idelity by modulating the proofreading 3' to 5' exonuclease activity.

111 at human NDK5, NDK7, and NDK8 contain 3' --> 5' exonuclease activity.

112 1 (NM23-H1) has been reported to have 3' --> 5' exonuclease activity.

113 on Klenow fragments with and without 3' --> 5' exonuclease activity.

114 Yet the mutants retain robust 3'-->5' exonuclease activity.

115 , both long isoforms were shown to have 3'-->5' exonuclease activity.

116 AN34 also displays enhanced 3'-5' exonuclease activity.

117 lls treated with STI-571 show increased 3'-->5' exonuclease activity.

118 The protein possesses Mg2+-dependent 3' to 5' exonuclease activity.

119 leading to their eventual removal by the 3'-5' exonuclease activity.

120 f the recQ family, it contains a unique 3'-->5' exonuclease activity.

121 ermediate product is then trimmed by a 3' -->5' exonuclease activity.

122 th 3'-->5' polarity, and also possesses 3'-->5' exonuclease activity.

123 icative DNA polymerase with an associated 3'-5' exonuclease activity.

124 sence of which severely diminishes its 3' to 5' exonuclease activity.

125 netics, strand displacement synthesis and 3'-5' exonuclease activity.

126 possesses single-stranded DNA and/or RNA 3'-5' exonuclease activity.

127 binds to single-stranded DNA and exhibits 3'-5' exonuclease activity.

128 for the quantitative determination of acidic 5' exonuclease activity.

129 Fourth, both the endonuclease activity and 5'-exonuclease activity of ARTEMIS can be blocked in par

130 recombinant protein we confirmed that the 3'-5'-exonuclease activity of Pol delta can efficiently rem

131 erminated 3'-end of the DNA primer by the 3'-5'-exonuclease activity of Pol gamma were similar (0.01

132 n uncertainty about whether ARTEMIS also has 5'-exonuclease activity on single-stranded DNA and 5'-ov

133 cleavage activity was not required for Exo1 5'-exonuclease activity on the lagging strand daughter D

134 e ability of Pol delta to back up via its 3'-5'-exonuclease activity, a process called idling, mainta

135 high fidelity owing to its intrinsic 3'- to 5'-exonuclease activity, which confers proofreading abil

136 a endonuclease V also possesses non-specific 5'-exonuclease activity.

137 NA editing exonuclease 1 (REX1), exhibits 3'-5'-exonuclease activity.

138 duces both the endonuclease activity and the 5'-exonuclease activity.

139 rase activity but only mildly impairs 3'- to 5'-exonuclease activity.

140 t the three biological functions of the 3'-->5' exonuclease addressed in this study are performed int

141 atalyzes three DNA-dependent reactions: a 3'-5'-exonuclease, an ATPase, and a 3'-5'-helicase.

142 a multifunctional nuclear protein with 3'-->5' exonuclease and 3'-->5' helicase activities.

143 Importantly, PCNA stimulates the 3'-->5' exonuclease and 3'-phosphodiesterase activities of Ap

144 in of Saccharomyces cerevisiae contains 3'-->5' exonuclease and 3'-phosphodiesterase activities, and

145 s a nuclear protein (WRN) which possesses 3'-5' exonuclease and ATPase-dependent 3'-5' helicase activ

146 sequential detection of the activities of 3'-5' exonuclease and DNase I in cell lysates.

147 Although the purified protein exhibits 3' to 5' exonuclease and endonuclease activities in vitro, Mre

148 We therefore examined WRN, a 3'-->5' exonuclease and helicase mutated in Werner syndrome,

149 We found that hRAD9 is a 3' to 5' exonuclease and located the nuclease active site to t

150 f its attendant enzymatic activities, the 3'-5' exonuclease and nucleoside diphosphate kinase, are no

151 ication by ISG20 and PKR depends on their 3'-5' exonuclease and protein kinase activities, respective

152 ciency of the WRN protein that harbors 3' -->5' exonuclease and RecQ-type 3' --> 5' helicase activiti

153 the Escherichia coli Klenow fragment (KF) 3'-5' exonuclease and snake venom phosphodiesterase.

154 The (R) isomer provided protection from 5' exonuclease and the (S) isomer provided protection fr

155 Here, we show that the 5'-exonuclease and the endonuclease activities co-purify

156 Third, divalent cation effects on the 5'-exonuclease and the endonuclease parallel one another

157 Human exonuclease 1 (hExo1) possesses both 5'exonuclease and flap endonuclease activities and plays

158 ) and recJ (5'-->3' exonuclease), xonA (3'-->5' exonuclease) and partially dependent on recQ (helicas

159 unit (polymerase), the epsilon subunit (3'-->5' exonuclease), and the theta subunit, in the linear or

160 e is folded into NH(2)- terminal, editing 3'-5' exonuclease, and polymerase domains that are topologi

161 ein complex composed of RET1 TUTase, DSS1 3'-5' exonuclease, and three additional subunits.

162 rmamido-pyrimidine-DNA glycosylase (FPG), 3'-5' exonucleases, and enzymes with template-independent t

163 3'-5' Exonucleases are believed to play an important role i

164 Related exosome complexes of 3'-->5' exonucleases are present in the nucleus and the cytop

165 we identified PNLDC1, an uncharacterized 3'-5' exonuclease, as Trimmer in silkworms.

166 Here, we describe the development of a 5' exonuclease assay for the detection of serogroup Y Ne

167 ng, direct sequencing, fluorescence-detected 5'-exonuclease assays, and hybridization with PNA probes

168 enzyme was originally identified as a 3' --> 5' exonuclease, but we show here that NrnA is bidirectio

169 e have defined NM23-H1 biochemically as a 3'-5' exonuclease by virtue of its ability in stoichiometri

170 ta long noncoding RNAs are protected from 3'-5' exonucleases by highly conserved triple helical struc

171 quired before RNase P and the various 3' --> 5' exonucleases can complete tRNA maturation.

172 TREX1 and TREX2 proteins contain potent 3'-->5' exonucleases capable of functioning in this capacity.

173 The 3'-->5' exonucleases catalyze the excision of nucleoside mono

174 Kinetic parameters for 3'-5' exonuclease cleavage of single- and double-stranded D

175 n this study we have investigated how 3' and 5' exonucleases contribute towards the successful termin

176 ggest the existence of a highly conserved 3'-5' exonuclease core domain within Rrp6p.

177 nase associates with exonuclease 1 (Exo1), a 5'-exonuclease crucial for 5'-end resection to mediate D

178 mer extension studies, catalyzed by the 3'-->5' exonuclease-deficient Klenow fragment of Escherichia

179 plate-directed nucleotide addition by the 3'-5' exonuclease-deficient large fragment of Escherichia c

180 ranscript acts by activating exosomal 3' --> 5'-exonuclease degradation activity.

181 siRNAs and the increased protection against 5'-exonuclease degradation afforded by the ANA modificat

182 e role of the Perlman syndrome-associated 3'-5' exonuclease Dis3l2 in rRNA processing.

183 R assays, we show here that the bacterial 3'-5' exonucleases DnaQ and ExoT can trim long 3' overhangs

184 Our findings implicate the E. coli host 3'-5' exonucleases DnaQ and ExoT in spacer adaptation and r

185 posed of an N-terminal DNA flap endonuclease/5' exonuclease domain (FEN/EXO) and a C-terminal DNA pol

186 tioning of DNA to the spatially separated 3'-5' exonuclease domain, providing an additional mechanism

187 EXD2 (3'-5' exonuclease domain-containing protein 2) is an essent

188 rotein with a conserved DEDDy superfamily 3'-5' exonuclease domain.

189 , owing to their lack of a proofreading (3'- 5' exonuclease) domain.

190 The 5'-exonuclease domains of the DNA polymerase I proteins

191 al degradosome, which also includes a 3' --> 5' exonuclease, Dss1p.

192 ed an about tenfold attenuation on the 3' to 5' exonuclease efficiency of Ape1.

193 a replicase generally have a more potent 3'-5' exonuclease (exo) activity than A family DNA polymera

194 DNA polymerases (Pol) have an intrinsic 3'-->5' exonuclease (Exo) activity which corrects polymerase

195 failures, result in substitutions in the 3'-5' exonuclease (Exo) domain of the catalytic subunit of

196 site, DNA polymerases have a separate 3' --> 5' exonuclease (exo) editing activity that is involved i

197 despite the presence of an associated 3'- to 5'-exonuclease (exo) activity.

198 y both the 5'-3' exonuclease RecJ and the 3'-5' exonuclease ExoI, observations that suggest the impor

199 other RNA viruses and is mediated by a 3'-->5' exonuclease (ExoN) activity that probably functions i

200 The association of DcpS with 3' to 5' exonuclease exosome components suggests that these tw

201 , and its intriguing regulation of the 3'-to-5' exonuclease exosome subunit suggests a potential inte

202 Genotyping for 10 SNPs by 5'-exonuclease fluorescence assays.

203 PAP I competes with the 3' -> 5' exonucleases for pre-tRNA substrates adding short pol

204 extension reactions catalyzed by the 3' --> 5' exonuclease-free (exo(-)) Klenow fragment of Escheric

205 er extension reactions catalyzed by the 3'-->5' exonuclease-free Klenow fragment of Escherichia coli

206 The cytosolic 3'-to-5' exonuclease from chronic lymphocytic leukemia cells w

207 ht into the mechanism for Nfo-catalyzed 3'-->5' exonuclease function and its inhibition by 3'-termina

208 op structure is readily attacked by the 3'-->5' exonuclease function of WRN.

209 Unlike gamma DNA polymerases, ablation of 3'-5' exonuclease function resulted in a modest 5-8-fold er

210 -phosphodiesterase, 3'-phosphatase, and 3'-->5'-exonuclease functions specific for the 3' termini of

211 e we identify oligoribonuclease (Orn)-a 3'-->5' exonuclease highly conserved among Actinobacteria, Be

212 ype 9 degrees N polD were used to examine 3'-5' exonuclease hydrolysis activity in the presence of Mg

213 ternucleotide linkages are resistant to 3'-->5' exonuclease hydrolysis, rendering the target DNA resi

214 action was necessary to protect PV mRNA from 5' exonuclease immediately as ribosomes initially traver

215 TOE1 may function non-redundantly as a 3'-to-5' exonuclease in conjunction with PARN.

216 death process implicating this major 3' --> 5' exonuclease in genomic DNA degradation to minimize po

217 eriments revealed PLD3 as the principal acid 5' exonuclease in HeLa cells, where it showed a markedly

218 chemical evidence that Isg20 acts as a 3' to 5' exonuclease in vitro.

219 o dnaQ, encoding the epsilon proofreading 3'-5'-exonuclease, interacts with alpha but does not form a

220 The yeast exosome is a complex of 3'-->5' exonucleases involved in RNA processing and degradati

221 RNase T and RNase PH, the two primary 3' --> 5' exonucleases involved in the final step of 3'-end mat

222 Nase T and/or RNase PH, the two major 3' --> 5' exonucleases involved in the final step of tRNA 3'-en

223 We conclude that the 5'-exonuclease is intrinsic to ARTEMIS, making it releva

224 -stranded DNA and 5'-overhangs, because this 5'-exonuclease is not dependent upon DNA-PKcs.

225 s in different biological matrices show that 5'-exonuclease is the most prevalent nuclease activity i

226 The protein is a single-strand 5'-exonuclease, like its yeast homolog.

227 Subsequently, a group of 3' -> 5' exonucleases mature the 3' ends of the majority of tR

228 tural substrates, indicating that the 3' --> 5' exonuclease may contribute to DNA synthesis inhibitio

229 ore than 100 bases per second and has a 3'-->5' exonuclease (nucleotide removing) activity at a separ

230 ercomes this inhibition to promote the 3' -> 5' exonuclease of MRX, which requires ATP hydrolysis by

231 ow that this mutation inactivates the 3' --> 5' exonuclease of poldelta and causes a mutator and canc

232 ea (MNU)-treated DNA templates by the 3' --> 5' exonuclease of T4 DNA polymerase.

233 genetic and biochemical analysis of the 3'-->5' exonuclease of yeast DNA polymerase delta (Pol delta)

234 the XPF-ERCC1 heterodimer, acted as a 3'-to-5' exonuclease on cross-linked DNA in the presence of RP

235 Escherichia coli, the effects of several 3'-5' exonucleases on RecA loading were studied by assaying

236 ally contained a DNA polymerase devoid of 3'-5' exonuclease, or "proofreading", activity blended with

237 The exosome complex of 3'-5' exonucleases participates in RNA maturation and quali

238 everse transcription-PCR and by an automated 5' exonuclease PCR.

239 Furthermore, ExoI, ExoIII and the other 3'-5' exonucleases process these DSBs, antagonizing the Rec

240 The 3' --> 5'-exonucleases process DNA ends in many DNA repair path

241 s from these mutants suggested limited 3'-to-5' exonuclease processing from the native 3' end.

242 GRF mutations impact APE2 DNA binding and 3'-5' exonuclease processing, and also prevent efficient AP

243 ibonucleotides escape proofreading by 3' --> 5' exonuclease-proficient Pol epsilon, indicating that r

244 sms (SNPs) that takes advantage of the 3'-to-5' exonuclease proofreading activity of many DNA polymer

245 -deficient (DeltaumuDC) strains if the 3' to 5' exonuclease proofreading activity of the Pol III epsi

246 Saccharomyces cerevisiae that possess 3' --> 5' exonuclease proofreading activity.

247 The 5'-exonuclease Rat1 degrades pre-rRNA spacer fragments a

248 on of the ARE or inactivation of the nuclear 5'-exonuclease Rat1.

249 that the N420 side chain facilitates the 3'-5' exonuclease reaction by introducing strain into the b

250 polymerases based on the analysis of its 3'-5' exonuclease reaction in the context of crystal struct

251 ree-prime repair exonuclease-1, a host 3' to 5' exonuclease, reduced IFN-beta expression significantl

252 and characterization of a nonexosomal, 3'-->5' exonuclease required for SL RNA 3'-end formation in T

253 Here we identify Dis3l2 as the 3'-5' exonuclease responsible for the decay of uridylated p

254 Here, we show that the 3'-->5' exonuclease RNase II plays an important role in RelE-

255 ecificity endoribonuclease RNase III and the 5' exonuclease RNase J1 are not essential in the Gram-po

256 s become standard in the field to use the 3'-5' exonuclease RNase R.

257 that does not involve any of the six 3' --> 5' exonucleases (RNase T, RNase PH, RNase D, RNase BN, R

258 ure revealed only one protein, another 3'-to-5' exonuclease, RNase II.

259 es, a DNA polymerization site (pol) and a 3'-5' exonuclease site (exo) for proofreading.

260 rtitioning of the DNA substrates into the 3'-5' exonuclease site by 3-7-fold, relative to the perfect

261 ielding a polymerizing complex, or to the 3'-5' exonuclease site, yielding an editing complex.

262 imer/template to the 5'-3' polymerase and 3'-5' exonuclease sites of KF.

263 revealed its association with Rrp6p, a 3'-->5' exonuclease specific to the nuclear exosome fraction.

264 nverted into 5' flaps, which are degraded by 5' exonucleases, such as ExoVII and RecJ.

265 ation of the DCP does not depend on the Xrn2 5' exonuclease, suggesting that CPSF-73 degrades the DCP

266 TREX2 is an autonomous nonprocessive 3' --> 5' exonuclease, suggesting that it maintains genome inte

267 tein is homologous with members of the 3' to 5' exonuclease superfamily that includes RNases T and D,

268 Furthermore, TREX2 (a 3'-->5' exonuclease) suppressed identical repeat fusion but e

269 B in tamarins was followed using a real-time 5' exonuclease (TaqMan) reverse transcription-PCR assay

270 approximately threefold and was the only 3'-5' exonuclease tested that did not deleteriously affect

271 he 5' end was more stable in the presence of 5'-exonuclease than an oligonucleotide of the same seque

272 e, in vitro and in vivo, that TdTL is a 3'-->5' exonuclease that catalyzes the deletion of nucleotide

273 TREX1 is a potent 3' --> 5' exonuclease that degrades single- and double-stranded

274 DNA replication depends on a proofreading 3'-5' exonuclease that is associated with the replicative D

275 e11 from Saccharomyces cerevisiae is a 3' -> 5' exonuclease that is responsible for 5' end degradatio

276 solated as a major nuclear DNA-specific 3'-->5' exonuclease that is widely distributed in both prolif

277 Exo5 is a monomeric 5' exonuclease that releases dinucleotides as products.

278 re with replication, whereas TREX2 is a 3'-->5' exonuclease that removes 3' mismatched nucleotides an

279 Trex2 is a 3' --> 5' exonuclease that removes 3'-mismatched sequences in a

280 aliana AtRrp4p is shown to be an active 3'-->5' exonuclease that requires a free 3'-hydroxyl and degr

281 als that DIS3L and DIS3L2 are critical 3' to 5' exonucleases that regulate miRNA stability, with the

282 Human EXOG (hEXOG) is a 5'-exonuclease that is crucial for mitochondrial DNA rep

283 We showed that these are U-specific 3'-5'-exonucleases that are inhibited by base pairing of 3'

284 stinct forms of a complex containing 10 3'-->5' exonucleases, the exosome, are found in yeast nucleus

285 y increased the ability of the enzyme's 3'-->5' exonuclease to remove mispaired 3' bases in a primer

286 ations identified in genes encoding the 3'-->5' exonuclease TREX1 and the three subunits of the RNASE

287 se DNA fragments and that the cytoplasmic 3'-5' exonuclease Trex1 is required for their degradation.

288 We now find the 3'-to-5' exonuclease TREX1, but not its close homolog TREX2, i

289 ntified the cDNA sequences encoding two 3'-->5' exonucleases (TREX1 and TREX2) from mammalian cells.

290 autonomous and DNA polymerase-associated 3'-5' exonucleases using a lacZ forward mutation assay.

291 A number of 3'-5' exonucleases were found to lower the error rate of MM

292 component of the exosome, a complex of 3'-to-5' exonucleases whose subunits have been implicated in 5

293 re members of a family of structure-specific 5'-exonucleases with similar function but limited sequen

294 how that the mouse WRN (mWRN) is also a 3'-->5' exonuclease, with substrate specificity similar to th

295 Transfected HCV RNA is degraded by both the 5' exonuclease Xrn1 and 3' exonuclease exosome complex,

296 dsRNA effector pathways and/or the cellular 5' exonuclease Xrn1 were prepared by CRISPR-Cas9 gene ed

297 Uncapped mRNAs are typically degraded by the 5' exonuclease XRN1.

298 ynthetic-lethal with loss of the cytoplasmic 5'-exonuclease Xrn1, indicating block of mRNA turnover,

299 We find that in mammalian cells, the 5' exonuclease Xrn2 plays a major role in both maturatio

300 the combination of I709F and lack of the 3'-5' exonuclease yielded a 400-fold increase.