ライフサイエンスコーパス: A. nidulans

1 ociation of the cargo adapter HookA (Hook in A. nidulans).

2 spore viability, and secondary metabolism in A. nidulans.

3 assa compared with that which has evolved in A. nidulans.

4 We find pinA to be an essential gene in A. nidulans.

5 hich is required for proper proliferation of A. nidulans.

6 DeltanudG) at the nudG locus encoding LC8 in A. nidulans.

7 the control of mitotic spindle formation in A. nidulans.

8 gesting that DHS and PHS induce apoptosis in A. nidulans.

9 UDE, which also affects nuclear migration in A. nidulans.

10 in a single step from a cell-free extract of A. nidulans.

11 sequenced from the model filamentous fungus A. nidulans.

12 fecting the asexual to sexual spore ratio in A. nidulans.

13 tty acid metabolism and spore development in A. nidulans.

14 it must be a dimer to support the growth of A. nidulans.

15 tibiotic (PN) and a lethal mycotoxin (ST) in A. nidulans.

16 dF, a gene required for nuclear migration in A. nidulans.

17 llus spp., are clustered on chromosome IV of A. nidulans.

18 us, and the sterigmatocystin gene cluster in A. nidulans.

19 t signal is required for apical extension in A. nidulans.

20 rowth, subapical cell arrest, and mitosis in A. nidulans.

21 (cdk) activity are required for septation in A. nidulans.

22 ts initiation of mitosis after DNA damage in A. nidulans.

23 t protein kinase (CaMK) is also essential in A. nidulans.

24 cies, including A. lentulus, A. terreus, and A. nidulans.

25 f phosphatidylserine to the Spitzenkorper in A. nidulans.

26 idines as representative formation of SMs in A. nidulans.

27 AC activity is, thus, spatially regulated in A. nidulans.

28 rowth, while represses sexual development in A. nidulans.

29 ite fungisporin, not previously described in A. nidulans.

30 tA and drives the sexual cycle in the fungus A. nidulans.

31 and germination by interacting with VosA in A. nidulans.

32 ng asexual development and conidiogenesis in A. nidulans.

33 elf-fertilization and sexual reproduction in A. nidulans.

34 cial for governing growth and development in A. nidulans.

35 eptation to take place in a timely manner in A. nidulans.

36 not play an important role in endocytosis in A. nidulans.

37 ttenuated by palB and pacC mutant strains of A. nidulans.

38 ation of a stable axis of hyphal polarity in A. nidulans.

39 ce between asexual and sexual development in A. nidulans.

40 is also functional when VeA is expressed in A. nidulans.

41 TR controls splicing of the arginase mRNA in A. nidulans.

42 as an antibiotic not known to be produced by A. nidulans.

43 cleaving a putative arginine riboswitch from A. nidulans.

44 us, 323 to 592 for A. flavus, 131 to 143 for A. nidulans, 366 to 520 for A. niger, 330 to 462 for A.

45 tages of agreement with reference values for A. nidulans (60 to 80%).

46 spergillus species (A. flavus, A. fumigatus, A. nidulans, A. niger, A. terreus, A. ustus, and A. vers

47 solates of Aspergillus fumigatus, A. flavus, A. nidulans, A. niger, and A. terreus to caspofungin (MI

48 t cases of invasive aspergillosis (IA), with A. nidulans, A. niger, and A. ustus being rare causes of

49 ntive structural annotation improvements for A. nidulans, A. oryzae and A. fumigatus genomes based on

50 typically heterogeneous but also differ from A. nidulans, A. spinulosporus, and A. quadrilineatus.

51 iger, A. terreus, A. versicolor, A. glaucus, A. nidulans, A. ustus, and A. sydowii.

52 In this report, we demonstrate that A. nidulans AflR (AnAflR) is a 45kDa protein that binds

53 Recently, A. nidulans AFLR was shown to bind to the motif 5'-TCGN5

54 ion of a Pseudogymnoascus destructans FAC in A. nidulans altered endogenous terpene biosynthetic path

55 olog of NUDE, a nuclear distribution gene in A. nidulans and a multicopy suppressor of the LIS1 homol

56 iated with the widespread SM changes in both A. nidulans and A. fumigatus during cocultivation.

57 pergillus species A. parasiticus, A. flavus, A. nidulans and A. sojae was conducted.

58 ciated with the production of eicosanoids in A. nidulans and Aspergillus fumigatus provides new insig

59 gical roles of the Pin1 orthologue, PINA, in A. nidulans and evaluate the relevance of the interactio

60 d that C-terminal domains of the full-length A. nidulans and Geobacillus stearothermophilus synthetas

61 to measure tip growth rates in germlings of A. nidulans and in multinucleate hyphal tip cells, and w

62 signaling was conserved in the genetic model A. nidulans and mediated by NapA, a homolog of AP-1-like

63 gh similarity to the pantothenate kinases of A. nidulans and mouse.

64 a conserved mechanism of nuclear movement in A. nidulans and neuronal migration in the developing mam

65 stabilities of the mitochondrial genomes in A. nidulans and P. anserina.

66 tor and NIMA are coincidentally regulated in A. nidulans and suggest that the unscheduled appearance

67 Surprisingly, wild-type A. nidulans and the catA, catB, and catA/catB mutants we

68 eins control similar morphogenetic events in A. nidulans and the dimorphic yeasts, significant differ

69 how NIMA promotes chromatin condensation in A. nidulans and when expressed in other eukaryotes.

70 regulatable promoter, transferring them into A. nidulans , and expressing them.

71 ases of A. fumigatus, A. flavus, A. terreus, A. nidulans, and A. oryzae for domains conserved in NRPS

72 isolates, and 10 isolates each of A. niger, A. nidulans, and A. terreus to voriconazole, posaconazol

73 We engineered ZmLOX3 into wild-type A. nidulans, and into a DeltappoAC strain that was reduc

74 A. nidulans appears to have a particular virulence in CG

75 constitutively active and inactive forms of A. nidulans Aras to modulate hyphal morphogenesis and as

76 co-transformation and complementation of an A. nidulans areA loss-of-function mutant (areA18 argB2 p

77 entation of their mutant phenotypes using an A. nidulans autonomously replicating vector.

78 functionally unassigned transcript, stcO, in A. nidulans based on sequence homology at both nucleotid

79 heterologous markers that are selectable in A. nidulans but do not direct integration at any site in

80 egulation of sexual development, not only in A. nidulans, but also in the phylogenetically unrelated

81 lus genus as genomic analysis indicates that A. nidulans, but not A. fumigatus or A. oryzae, has lost

82 ination during transformation is possible in A. nidulans, but the frequency of correct gene targeting

83 ulle cells in establishing secure niches for A. nidulans by accumulating metabolites with antifeedant

84 Conidiation induction in A. nidulans by another microbial redox-active secondary

85 Therefore SVs arrive at the apex of A. nidulans by anterograde transport involving cooperati

86 This indicates that A. nidulans cells ensure accurate mitotic NPC segregatio

87 tion, and the requirement for such a role in A. nidulans cells is temperature dependent.

88 t nearly all ability to synthesize the major A. nidulans characteristic terpene, austinol.

89 Here, we have identified the A. nidulans CLIP-170 homologue, CLIPA.

90 ve binding affinities within the cell during A. nidulans' closed mitosis, analogous to what occurs du

91 chanism by which the NPC is regulated during A. nidulans' closed mitosis.

92 ded polypeptides are 41-43% identical to the A. nidulans CRNA protein and 56-57% identical to NAR-3,

93 ion of rca-1 caused conidiation in submerged A. nidulans cultures just as was previously observed for

94 Mutations that disrupt tagging, including A. nidulans cutA and a newly characterized gene, cutB, r

95 In A. nidulans, deletion of rcoA (DeltarcoA), a recessive m

96 The A. nidulans disordered protein Spa18(Mto2) and the centr

97 SB functions as the central regulator of the A. nidulans DNA damage response, whereas MUSN promotes r

98 lization suggest that PrpA acts early in the A. nidulans DNA damage response.

99 e, we demonstrate that SepB functions in the A. nidulans DNA damage response.

100 Because A. nidulans does not secrete detectable amounts of farne

101 well as other mitotic genes, indicates that A. nidulans dynein plays a role in mitosis.

102 In A. nidulans, dynein is not apparently required for mitot

103 A total of 5.6% of A. nidulans ESTs implicate inducer-dependent cell wall d

104 Stress response genes in A. nidulans ESTs total 1039 transcripts, contrasting wit

105 These data also reveal that A. nidulans exhibits a remarkable spatial regulation of

106 The redox and structural properties of A. nidulans flavodoxin and the Asn58Gly mutant confirm t

107 e potentials and the effects of mutations in A. nidulans flavodoxin are rationalized using a thermody

108 ents of three orthorhombic forms of oxidized A. nidulans flavodoxin are reported, and salient feature

109 tudied the backbone mobility of the oxidized A. nidulans flavodoxin at pH 6.6, 303 K by 15N NMR relax

110 Asn58-Val59 peptide in crystalline wild-type A. nidulans flavodoxin rotates away from the flavin to t

111 gene appears to be a functional homologue of A. nidulans flbD and this is the first demonstration of

112 can complement the conidiation defect of an A. nidulans flbD mutant and that induced expression of r

113 We have used A. nidulans for our method development and validation du

114 Therefore, an essential function exists in A. nidulans for the Pho85-like kinase pair PHOA and PHOB

115 show here that loss of either FhipA or FtsA (A. nidulans FTS homologue) disrupts HookA-early endosome

116 in polar growth and nuclear distribution in A. nidulans, functions not yet described for its homolog

117 f A. terreus; one isolate each of A. flavus, A. nidulans, Fusarium moniliforme, and F. solani; and tw

118 We propose that GpgA is the only A. nidulans G gamma-subunit and is required for normal v

119 enomic clone and the characterization of the A. nidulans gene designated panK.

120 One mutation, an unprecedented finding in A. nidulans genetics, resulted from an insertion of an e

121 We have characterized a 60-kb region in the A. nidulans genome and find it contains many, if not all

122 Analysis of the A. nidulans genome identified a single gene named gpgA e

123 petitive DNA is nonrandomly dispersed in the A. nidulans genome, reminiscent of heterochromatic bandi

124 do not direct integration at any site in the A. nidulans genome.

125 activated form of rasA, the ras homologue in A. nidulans, germinate in the absence of an inducing car

126 Wild-type A. nidulans germinated on porcine corneas and produced h

127 The genome of A. nidulans harbors genes for the biosynthesis of xantho

128 We conclude that A. nidulans has components of a SIN-MEN pathway, one of

129 4.2 crossovers per chromosome pair, whereas A. nidulans has in contrast a higher rate with 9.3 cross

130 In this study the genetic model organism, A. nidulans, has been used to investigate the regulation

131 We also demonstrate that the single A. nidulans histone H2A gene contains the C-terminal SQE

132 The predicted SUDD proteins from A. nidulans, Homo sapiens and S. cerevisiae all share a

133 We have identified the A. nidulans homolog (nkuA) of the human KU70 gene that i

134 hen combined with mutations in scaANBS1, the A. nidulans homolog of NBS1.

135 We have investigated the role of CdhA, the A. nidulans homologue of the APC/C activator protein Cdh

136 ergillus nidulans as a key player for HookA (A. nidulans Hook) function via a genome-wide screen for

137 te host-defense pathway, the pathogenesis of A. nidulans in CGD cannot be explained.

138 play a significant role in pathogenicity of A. nidulans in p47(phox)-/- mice, and therefore raise do

139 derstanding of invasive infections caused by A. nidulans in the CGD patient and is intended to direct

140 e lungs of LS patients and suggest a role of A. nidulans in the etiology of LS.

141 Using A. nidulans in vivo microscopy, we show that HypA(Trs120

142 Wild-type A. nidulans inoculated intranasally caused fatal infecti

143 2 is required for mitotic NPC inheritance in A. nidulans Interestingly, the role of Nup2 during mitot

144 RNA silencing is not a significant aspect of A. nidulans IRT-RNA silencing.

145 trate that our newly identified dynein IC in A. nidulans is also localized to microtubule ends and is

146 As A. nidulans is genetically tractable, this organism shou

147 These data suggest nitrogen metabolism in A. nidulans is in part regulated in response to the intr

148 tial and positively regulates NIMA function, A. nidulans is most sensitive to a reduction in PINA con

149 model depicting regulation of conidiation in A. nidulans is presented.

150 de that the essential role(s) of myosin I in A. nidulans is probably structural, requiring little, if

151 activator protein for quinate catabolism in A. nidulans is that expected for random sequences of the

152 nazole, 0.25 (95%); voriconazole, 1 (98.1%); A. nidulans, itraconazole, 1 (95%); posaconazole, 1 (97.

153 iticus, and sterigmatocystin biosynthesis in A. nidulans, led to the cloning of 17 genes responsible

154 murine immunity contributes significantly to A. nidulans lethality.

155 mutation suppresses the growth defect of the A. nidulans LIS1-deletion mutant.

156 isassembly under control of NIMA and Cdk1 in A. nidulans may represent a new mechanism for regulating

157 pose a complete biosynthetic pathway for the A. nidulans meroterpenoids.

158 t overexpressed cargo adapter HookA (Hook in A. nidulans) missing its cargo-binding domain (DeltaC-Ho

159 e dramatic changes in NPC composition during A. nidulans mitosis and provides insight into how NPC di

160 up of the NPC is dramatically changed during A. nidulans' mitosis.

161 at DHS and PHS induce a type of apoptosis in A. nidulans most similar to the caspase-independent apop

162 he marginally altered phenotypes observed in A. nidulans mutants indicate the presence of effective c

163 ns wild-type isolate (A83), loss-of-function A. nidulans mutants of the palB (B7) or pacC (C6309) gen

164 Finally, increased IgG antibody responses to A. nidulans NDPD were detected in the serum of DR3+ LS s

165 isolated four extragenic suppressors of the A. nidulans nimX2(cdc2) temperature-sensitive mutation.

166 his paper we examine the interactions of the A. nidulans NUDF and NUDE proteins with components of dy

167 report that the FluG-mediated conidiation in A. nidulans occurs via derepression.

168 e (CGD) is Aspergillus fumigatus followed by A. nidulans; other aspergilli rarely cause the disease.

169 We additionally explored whether A. nidulans oxylipins affect seed LOX gene expression du

170 The amino acid sequence of A. nidulans PanK (aPanK) predicted a subunit size of 46.

171 stimulates transcription of a gene from the A. nidulans penicillin (PN) gene cluster and elevates pe

172 to enhance virulence, demonstrating that the A. nidulans pH-responsive transcription factor PacC play

173 hat, in neutropenic mice, elimination of the A. nidulans pH-responsive transcription factor PacC, blo

174 Uniquely among the A. nidulans pH-signalling pal genes, palC appears to hav

175 ome-specific library and correlation with an A. nidulans physical map, the septins are not clustered

176 Moreover, A. nidulans possesses a second likely ceramide synthase

177 on-mammalian genomes, and the discovery that A. nidulans possesses reading frames so closely homologo

178 e (ZmLOX3) could substitute functionally for A. nidulans ppo genes.

179 Here, we report the identification of A. nidulans ppoA, encoding a putative fatty acid dioxyge

180 Therefore, in A. nidulans, proteins encoded by the smo genes likely ha

181 l. (2014) describe important new findings in A. nidulans regarding the role of EBA, the master regula

182 fl bA encodes an A. nidulans regulator of G-protein signaling (RGS) domai

183 his study shows that conidial germination in A. nidulans requires protein synthesis and that the init

184 tion of the 27 polyketide synthases (PKS) in A. nidulans revealed that one highly reduced PKS (HR-PKS

185 sequencing of Aspergillus species including A. nidulans reveals that the products of many of the sec

186 The A. nidulans rtfA gene product accumulates in nuclei.

187 In A. nidulans, salt stress HOG genes, such as pbsA, hogA,

188 Since no known A. nidulans secondary metabolites could be produced by t

189 As expected, the A. nidulans septins contain the highly conserved GTP bin

190 In A. nidulans, septum formation requires the assembly of a

191 Here, we show that in A. nidulans several SPB outer plaque proteins also locat

192 lso developed procedures for deleting entire A. nidulans SM clusters.

193 Although comparable to S. pombe eMTOCs, A. nidulans sMTOCS are permanent septum-associated struc

194 hese activities may be sufficient to prevent A. nidulans spores from entering into DNA synthesis.

195 stration of functional complementation of an A. nidulans sporulation defect using a gene from an evol

196 A. nidulans stcU was shown previously to encode a ketore

197 s observation led us to hypothesize that the A. nidulans sterigmatocystin biosynthetic pathway is bra

198 P-body formation is disrupted in A. nidulans strains deleted for Edc3, an enhancer of dec

199 In vitro growth kinetics were similar for A. nidulans strains in liquid medium at pH 6.0 (P = 0.24

200 n of scarified porcine or human corneas with A. nidulans strains maintained in buffered medium until

201 GUS activity in wild-type aflR or delta aflR A. nidulans strains, we found that stc gene activation r

202 nding motif present in related proteins from A. nidulans (StuA), Candida albicans (EFGTF-1), and Sacc

203 effect on cellular physiology and ageing in A. nidulans than that of their homologs in another fungu

204 e suggest a model for dynein motor action in A. nidulans that can explain dynein involvement in both

205 hile deletion of the swoC gene was lethal in A. nidulans, the C terminus, including NLS, microtubule-

206 of principle, we have engineered strains of A. nidulans to synthesize the fungal secondary metabolit

207 During asexual development, A. nidulans unexpectedly accumulates stress response and

208 clear localization signal (NLS) motif in the A. nidulans VeA amino acid sequence and demonstrated its

209 re microtubule dynamics in vivo in wild-type A. nidulans versus temperature-sensitive loss-of-functio

210 impaired in a farnesol-dependent manner when A. nidulans was co-cultivated with C. albicans.

211 The sepH gene from A. nidulans was discovered in a screen for temperature-s

212 Similar to the CGD model, catalase-deficient A. nidulans was highly virulent in cortisone-treated BAL

213 ant VdtB and VdtD as cell-free extracts from A. nidulans, we demonstrated that VdtD acts like a dirig

214 ation of the pathway metabolite scytalone in A. nidulans, we provided chemical evidence that the Pfma

215 ate the complete range of dynein function in A. nidulans, we searched for synthetic lethal mutations

216 ultiple silent biosynthetic gene clusters in A. nidulans were activated by transcriptome and metabolo

217 s found to be highly homologous to stcP from A. nidulans, which has been reported earlier to be invol

218 ed by heterologous pathway reconstitution in A. nidulans, which led to biosynthesis of intermediates

219 ine a second and specific RGS-Galpha pair in A. nidulans, which may govern upstream regulation of fun

220 nown, and will then juxtapose N. crassa with A. nidulans, which, as will be described below, provides

221 Fungal strains included an A. nidulans wild-type isolate (A83), loss-of-function A.

222 combination deficient (nkuADelta) strains of A. nidulans with fusion PCR products results in high fre