ライフサイエンスコーパス: AP lyase

1 idine hydrate-thymine glycol DNA glycosylase/AP lyase.

2 Ku can be identified as an effective 5'-dRP/AP lyase.

3 al glycosylase to a bifunctional glycosylase/AP lyase.

4 termediate characteristic of DNA glycosylase/AP lyases.

5 ndonuclease III and related DNA glycosylases/AP lyases.

6 a reaction characteristic of DNA glycosylase/AP lyases.

7 of action for this class of DNA glycosylase/AP lyases.

8 sed for several glycosylases and glycosylase/AP lyases.

9 he first one and to the sites found in other AP lyases.

10 e-limiting step catalyzed by BER glycosylase:AP-lyases.

11 step is catalyzed by DNA glycosylase/abasic (AP) lyases.

12 e repaired reside in DNA glycosylase/abasic (AP) lyases.

13 ncode a protein that possesses N-glycosylase/AP lyase activities for the repair of oxidatively damage

14 ated their DNA cleavage, DNA glycosylase and AP lyase activities in vitro at 37 degrees C.

15 the Michaelis constants for both the dRP and AP lyase activities of beta-pol are similar.

16 l extract by affecting the base excision and AP lyase activities of NEILs.

17 ious genetic studies have suggested that the AP lyase activities of Ntg1p and Ntg2p may be major cont

18 ducted a biochemical characterization of the AP lyase activities of Ntg1p and Ntg2p via a series of k

19 E23D retained near wild type glycosylase and AP lyase activities on cis-syn dimers but completely los

20 tes, including those of DNA N-glycosylase or AP lyase activities that are modulated by previously rep

21 Some DNA glycosylases possess AP lyase activities that nick the DNA strand at the deox

22 p26 contains both DNA glycosylase and AP lyase activities, and we provide evidence for a react

23 without affecting either the glycosylase or AP lyase activities, or the steady-state equilibrium con

24 dispensable for NEIL1's lesion excision and AP lyase activities, this segment is required for effici

25 on to Arg inactivates both base excision and AP lyase activities, while acetylation of Lys153 has no

26 minimal fragment with both topoisomerase and AP lyase activities.

27 (alpha)R61K(beta) showed slight reduction in AP lyase activities.

28 t pyrimidine hydrates and thymine glycol and AP lyase activity (DNA strand cleavage at AP sites via b

29 estingly, there is a concomitant increase in AP lyase activity above wild-type for the K155A mutant (

30 Addition of APE1 did not abrogate hNTH1 AP lyase activity against Tg:G.

31 bset of glycosylases that have an associated AP lyase activity also catalyze DNA strand breakage at t

32 erated AP:A site, resulting in abrogation of AP lyase activity and an increase in turnover of the DNA

33 the base excision activity and increases the AP lyase activity as well.

34 E3D, E3Q, and E174Q retained significant AP lyase activity but had severely diminished or abolish

35 its substrate and increases its glycosylase/AP lyase activity by a factor of approximately 2 through

36 Glycosylase/ap lyase activity explains MAP30's apparent inhibition o

37 t detect a backup glycosylase or glycosylase/AP lyase activity for thymine glycol in the mitochondria

38 gh temperature while another showed its weak AP lyase activity generates atypical ends.

39 well studied, the in vivo importance of the AP lyase activity has not been determined.

40 al relevance of the N-glycosylase-associated AP lyase activity in the repair of abasic sites is not w

41 The Ku AP lyase activity is also strongly suppressed by as litt

42 by a small alpha-helical domain, whereas the AP lyase activity is found in a region formed by 12 tand

43 The AP lyase activity is more coupled with glycosylase activ

44 shown that DNA repair proteins that possess AP lyase activity leave an inefficient DNA terminus for

45 The AP lyase activity of all of the glutamic acid mutants wa

46 A model is proposed whereby the AP lyase activity of DNA ligase may contribute to the re

47 ls, the 5'-sugar phosphate is removed by the AP lyase activity of DNA polymerase beta (Pol beta).

48 ies are of the same specific activity as the AP lyase activity of hNTH1 against Tg:A.

49 xyuracil residue as substrates, we found the AP lyase activity of hNth1 was 7 times slower than its D

50 High levels of the 5'-dRP/AP lyase activity of Ku are thus restricted to substrate

51 The AP lyase activity of mOgg1 is significantly lower than i

52 ause of higher AP site affinity and stronger AP lyase activity of NEIL1 relative to OGG1.

53 Apn1p suggests a physiological role for the AP lyase activity of Ntg1p and Ntg2p in vivo.

54 The AP lyase activity of T4 DNA ligase is inhibited in the p

55 kcat is approximately 200-fold lower for the AP lyase activity on an intact AP site than for an AP en

56 e p26 domain retains adenine glycosylase and AP lyase activity on DNA containing undamaged adenine op

57 sed to show that the enzyme also exhibits an AP lyase activity on UV- and gamma-irradiated DNA substr

58 It also possesses an AP lyase activity that cleaves at a deoxyribose but not

59 veral reports in the literature that ascribe AP lyase activity to MutY.

60 Bifunctional glycosylase/AP lyase activity was successfully engineered into MutY

61 activity of hNTH1 is 7-fold greater than its AP lyase activity when the DNA substrate contains a thym

62 intact apurinic/apyrimidinic (AP) site (i.e. AP lyase activity).

63 e of the DNA with uracil DNA glycosylase, an AP lyase activity, and ligation-mediated PCR to map the

64 accharomyces cerevisiae also possesses 5'dRP/AP lyase activity, and robust activity was also reliant

65 ROS1 is slow in base excision and fast in AP lyase activity, indicating that the recognition of py

66 ine-DNA glycosylase 1 (OGG1), with intrinsic AP lyase activity, is the major enzyme for repairing 7,8

67 nd purified from E. coli has DNA glycosylase/AP lyase activity, primarily for excising oxidative prod

68 al DNA glycosylases that possess concomitant AP lyase activity, was also substantially reduced when M

69 pair pathways via its DNA glycosylase and/or AP lyase activity, which are considered canonical roles

70 tivity completely but retained low levels of AP lyase activity.

71 with enzymes having bifunctional glycosylase/AP lyase activity.

72 sentative ATP-dependent DNA ligase, contains AP lyase activity.

73 active for base excision but retains partial AP lyase activity.

74 s with such damage similarly requires 5'-dRP/AP lyase activity.

75 mutant protein exhibited significant loss in AP lyase activity.

76 oxidized pyrimidines, although it maintained AP lyase activity.

77 ty of nth1 cells appears to be their lack of AP lyase activity.

78 h1 increased the rate of DNA glycosylase and AP lyase activity.

79 DNA remains due to lack of an efficient Myh AP lyase activity.

80 to U/G and T/G mismatches with an uncoupled AP lyase activity.

81 ites are responsible for MAP30's glycosylase/ap lyase activity.

82 are dispensable for its DNA glycosylase and AP lyase activity; however, the potential function of th

83 ntly reported for the apurinic/apyrimidinic (AP) lyase activity associated with Drosophila ribosomal

84 A glycosylase with an apurinic/apyrimidinic (AP) lyase activity encoded by the Escherichia coli endon

85 S3 also possesses an apurinic/apyrimidinic (AP) lyase activity in which the enzyme catalyzes a beta-

86 contain an associated apurinic/apyrimidinic (AP) lyase activity that cleaves phosphodiester bonds via

87 ses has an associated apurinic/apyrimidinic (AP) lyase activity that further processes the AP site to

88 phosphate (5'dRP) and apurinic/apyrimidinic (AP) lyase activity, and showed this activity is importan

89 ontains an associated apurinic/apyrimidinic (AP) lyase activity, cleaving phosphodiester bonds via a

90 ciated with its known apurinic/apyrimidinic (AP) lyase activity.

91 h DNA glycosylase and apurinic/apyrimidinic (AP) lyase activity.

92 6kDa DNA glycosylase with associated abasic (AP) lyase activity.

93 cosylase with abasic (apurinic/apyrimidinic (AP)) lyase activity and specifically cleaves oxidatively

94 glycosylase activity without any detectable AP-lyase activity during the first 10 min of the reactio

95 D50 can cleave DNA at AP sites and that this AP-lyase activity is conserved from humans to Archaea.

96 g 3' phosphate) generated by DNA glycosylase/AP lyases after base excision.

97 mitochondrial DNA, whereas other glycosylase/AP lyases also participate in removing other oxidized py

98 MAP30 acts like a DNA glycosylase/apurinic (ap) lyase, an additional activity distinct from its know

99 hemical information from several glycosylase/AP lyases and the structural information on the bacterio

100 ic bond (DNA glycosylase), beta-elimination (AP lyase), and delta-elimination; these functions act in

101 DNA glycosylase and glycosylase/abasic (AP) lyases are the enzymes responsible for initiating th

102 n repair (BER), the bifunctional glycosylase/AP lyases as well as AP endonuclease, were significantly

103 These bifunctional glycosylase/AP lyases bear a conserved lysine group in the active si

104 mOgg1 is a DNA glycosylase/AP lyase belonging to the endonuclease III family of DNA

105 (nfo), indicating for the first time that an AP lyase can represent a significant source of DNA repai

106 Ku, a 5'-dRP/AP lyase, can excise such damage at ends in preparation

107 hH DNA glycosylases and combined glycosylase/AP lyases cannot be interconverted by simply altering th

108 Similar to other glycosylase/AP lyases, catalysis by Fpg is known to proceed by a nuc

109 NA glycosylases and glycosylase-abasic site (AP) lyases cleave the glycosyl bond.

110 in DNA that are generated by DNA glycosylase/AP-lyases during removal of oxidized bases and by direct

111 se (hydrolysis of the N-glycosidic bond) and AP lyase (elimination of the 3'-phosphate of the AP-site

112 tent with the model proposed for glycosylase/AP lyase enzymes in which the glycosylase action is medi

113 Upon encountering dL, AP lyase enzymes such as DNA polymerase beta (Polbeta) f

114 This 5'-dRP group may be removed by AP lyase enzymes that employ a beta-elimination mechanis

115 Prokaryotic DNA glycosylase/AP lyases function through N-acylimine (Schiff's base) E

116 the DNA N-glycosylase/apurinic/apyrimidinic (AP) lyase, human NTH1 (hNTH1), the homolog of Escherichi

117 jor DNA N-glycosylase/apurinic/apyrimidinic (AP) lyases involved in the repair of oxidative base dama

118 might be operative for other BER glycosylase:AP-lyases, molecular modeling studies were conducted com

119 Nth and both known mammalian DNA glycosylase/AP lyases, namely, OGG1 and NTH1.

120 ecently discovered mammalian DNA glycosylase/AP lyases, NEIL1 and NEIL2, unlike the previously charac

121 f the Saccharomyces cerevisiae N-glycosylase/AP lyases, Ntg1 and Ntg2, in response to nuclear and mit

122 excision repair (BER) enzymes N-glycosylase/AP lyase OGG1 and APE/Ref-1.

123 ses and is therefore either a monofunctional AP lyase or may be a glycosylase-lyase on a yet unidenti

124 t previously excised by OGG1 DNA glycosylase/AP lyase proteins in eukaryotes.

125 ated by N-glycosylase apurinic/apyrimidinic (AP) lyase proteins.

126 nd 8-aminoguanine, trigger the rate-limiting AP lyase reaction.

127 12 tandem (HhH)(2) domains, which include 3 AP lyase repair domains.

128 ctivities and is also unique by having three AP lyase repair sites in the same polypeptide.

129 The 3rd AP lyase site is located in the 12th (HhH)2 domain.

130 Here, we show that Topo-V has three AP lyase sites.

131 Here we show that NEIL1, a DNA glycosylase/AP lyase specific for many oxidized bases but with weak

132 EIL2 (Nei-like-2) protein, a DNA glycosylase/AP lyase specific for oxidatively damaged bases, shares

133 ribonolactone is not incised by any of the 8 AP lyases tested.

134 The hOGG1 gene encodes a DNA glycosylase/AP lyase that can suppress the mutagenic effects of 8-hy

135 This enzyme is the glycosylase/AP lyase that participates in repair of purine lesions,

136 E. coli endonuclease III, a DNA glycosylase/AP lyase that repairs oxidatively damaged pyrimidines.

137 removed from DNA by hOgg1, a DNA glycosylase/AP lyase that specifically incises 8-oxoG opposite cytos

138 is a DNA glycosylase/apurinic/apyrimidinic (AP) lyase that initiates base excision repair of pyrimid

139 NA glycosylase and 3' apurinic/apyrimidinic (AP) lyase that is active on DNA substrates containing A/

140 T4 endonuclease V is a glycosylase/apurinic (AP) lyase that is specific for UV light-induced cis-syn

141 lycosylase-associated apurinic/apyrimidinic (AP) lyases that recognize a wide variety of damaged pyri

142 A repair enzymes that catalyze N-glycosylase/AP lyase-type reactions at sites of DNA damage.

143 mic screening, we discovered a new family of AP lyases typified by TK0353.

144 In summary, TK0353 is a novel AP lyase unique to hyperthermophiles that provides redun

145 assification of gelonin as a DNA glycosylase/AP lyase using the borohydride trapping assay revealed t

146 The function of HU protein as an AP lyase was confirmed by the ability of hupA or hupB mu

147 overexpression of two bacterial glycosylase/AP lyases which predominantly remove oxidized pyrimidine

148 iated with their excision by DNA glycosylase/AP lyases, which are of two classes.