ライフサイエンスコーパス: AQP

1 AQP deletion did not significantly affect W/D at 45 min

2 AQP inhibition may thus reduce the metastatic potential

3 AQP-0 was located almost exclusively in DSMs at a 1:1200

4 AQP-1 enhances osmotic water permeability and FGF-induce

5 AQP-1 expression and localization was examined in normal

6 AQP-1 in young reticulocytes localizes to the plasma mem

7 AQP-1 levels were modulated in LEC using retroviral over

8 AQP-1 overexpression promotes fibroblast growth factor (

9 AQP-2 levels were lower during early postnatal life, rea

10 AQP-2 possesses a single consensus cAMP-dependent protei

11 AQP-2 protein expression was also upregulated, but was s

12 AQP-2 urinary excretion changed with short-term alterati

13 the eye lens, the water channel aquaporin-0 (AQP-0) and the connexins Cx46 and Cx50, are preferential

14 hat RD alters the expression of aquaporin-0 (AQP-0), and this modulation is prevented by treatment wi

15 to investigate the function of Aquaporin 1 (AQP-1) signaling.

16 Aquaporin-1 (AQP-1), the universal water channel, is responsible for

17 cated almost exclusively in DSMs at a 1:1200 AQP-0/lipid ratio, whereas approximately 50% of the prot

18 downregulation of whole kidney aquaporin-2 (AQP-2) protein and mRNA expression.

19 ed insertion and removal of the aquaporin-2 (AQP-2) water channel.

20 Aquaporin-2 (AQP-2), a water channel located on the apical membrane o

21 To define the role of aquaporin-2 (AQP-2), the developmental expression of this water chann

22 pressin-sensitive water channel aquaporin-2 (AQP-2).

23 ressin-sensitive water channel (aquaporin 2; AQP-2) mediates water transport across the apical plasma

24 Aquaporin-2-4 (AQP) are expressed in the principal cells of the renal c

25 that an increased expression of aquaporin-4 (AQP-4) in the flight mice compared to controls gave stro

26 Additionally, AQP-1 localizes to plasma membrane blebs, where it incre

27 The GFP fusion did not affect AQP tetrameric association or water transport function.

28 om this study, it is concluded that although AQP-2 expression may play a role in the development of u

29 affect glycerol and urea permeability in an AQP-specific manner.

30 logy of hKID to human MIP (48% identity) and AQP-2 (52%), with lesser homology to human MIWC (AQP-4,

31 identical chromosomal loci of hKID, MIP, and AQP-2 suggest a MIP family gene cluster at chromosome lo

32 Among those genes, AMT, NRT and AQP for N uptake and GOGAT and GS for N assimilation wer

33 on, diffusional water permeability (Pd), and AQP expression.

34 t there were no differences in wild-type and AQP knockout mice.

35 omparably in mitochondria from wild-type and AQP-deficient mice, although the slowing was explained b

36 in low amounts, we hypothesized that another AQP must be expressed at this domain to facilitate trans

37 Aquaporin (AQP) 4 is the predominant water channel in the mammalian

38 Aquaporin (AQP) 6 belongs to the aquaporin water channel family.

39 Aquaporin (AQP) is a hexahelical integral membrane protein that fun

40 Aquaporin (AQP) water channel AQP3 has been proposed to be the majo

41 Aquaporin (AQP) water channel proteins are tetrameric assemblies of

42 Aquaporin (AQP) water channels are abundant in the brain and spinal

43 Aquaporin (AQP) water channels are expressed in high-grade tumor ce

44 Aquaporin (AQP) water channels are found throughout nature and conf

45 Aquaporin (AQP) water channels facilitate water transport across ce

46 Aquaporin (AQP) water channels provide a major pathway for osmotica

47 Aquaporin (AQP) water-channel proteins are freely permeated by wate

48 Aquaporin (AQP)-1 is a hexahelical integral membrane protein that f

49 Aquaglyceroporin aquaporin (AQP)3 is the major glycerol channel in human and rat ery

50 colocalize with the water channel aquaporin (AQP) 4 in prominin-1 immunopositive (P(+)) precursors in

51 Water channel aquaporin (AQP)-4 is expressed in Muller cells in retina, which are

52 cking each of the water channels, aquaporin (AQP)-1, -3, and -5, normally expressed in cornea or conj

53 sed upstream and in-frame to each aquaporin (AQP).

54 Hypertonic aquaporin (AQP) induction has been described, but little is known a

55 cluded that amiloride-inhibitable aquaporin (AQP) water channels in airway epithelia modulate airway

56 how that the basolateral membrane aquaporin (AQP)-3, but not the equivalent apical membrane AQP5, is

57 We have cloned a novel aquaporin (AQP) from Xenopus laevis oocytes, which we have provisio

58 ough the insertion and removal of aquaporin (AQP) 2 water channels into the IMCD apical membrane.

59 results of a multicentre study of aquaporin (AQP) 4 antibody (AQP4-Ab) assays in neuromyelitis optica

60 can be used to study the roles of aquaporin (AQP) in cellular water permeability and screen AQP-speci

61 The function of aquaporin (AQP) protein in transporting water is crucial for plants

62 ated with decreased expression of aquaporin (AQP)-2 in the renal inner medulla.

63 reports suggest the expression of aquaporin (AQP)-type water channels in mitochondria from liver (AQP

64 tion of the water channel protein aquaporin (AQP).

65 er of the major intrinsic protein/aquaporin (AQP) channel family, is a major component of the soybean

66 Previous studies reported aquaporin (AQP)-3 expression in cysts derived from collecting ducts

67 Immunocytochemistry showed strong aquaporin (AQP)-4 water channel expression in Muller cells in mouse

68 The aquaporin (AQP) family of integral membrane protein channels mediat

69 lar water flow is mediated by the aquaporin (AQP) family of membrane proteins.

70 emanating from the center of the aquaporin (AQP) water and solute channel is responsible for the rep

71 ulin 26 (NOD 26), a member of the aquaporin (AQP) water channel family, is a major protein component

72 Two aquaporin (AQP)-type water channels are expressed in mammalian corn

73 We show here that, in vitro, aquaporin (AQP) blockade or deficiency results in reduced IL-1beta

74 n might include interactions with aquaporin (AQP) water channel isoforms, although the proposed requi

75 by 56% to 58% by coinjection with aquaporin (AQP)5 antisense oligonucleotide.

76 Aquaporin- (AQP) 3, a water and glycerol channel, plays an important

77 Aquaporins (AQP) are members of the major intrinsic protein (MIP) su

78 Aquaporins (AQP) are water-specific membrane channel proteins.

79 f water channels, referred to as aquaporins (AQP): AQP0-AQP9.

80 es that cellular water channels, aquaporins (AQP), are central to both processes.

81 erol permeabilities of mammalian aquaporins (AQP) 1-5 and the major intrinsic protein of lens fiber (

82 ed previously that expression of aquaporins (AQP) 1, 4, and 5 in rat lung increased just after birth.

83 ember of the tonoplast family of aquaporins (AQP).

84 Aquaporins (AQPs) are a family of water permeable channels expressed

85 Aquaporins (AQPs) are a ubiquitous family of transmembrane water cha

86 Aquaporins (AQPs) are biological water channels known for fast water

87 Aquaporins (AQPs) are channel proteins that regulate the movement of

88 Aquaporins (AQPs) are important in controlling water permeability.

89 Aquaporins (AQPs) are integral membrane proteins whose function is t

90 Aquaporins (AQPs) are integral membrane water channels, recognized f

91 Aquaporins (AQPs) are transmembrane water channels ubiquitously expr

92 Aquaporins (AQPs) are water channel proteins that are essential in b

93 Aquaporins (AQPs) are water channels allowing fast and passive diffu

94 Aquaporins (AQPs) are water channels that mediate a variety of biolo

95 Aquaporins (AQPs) in the major intrinsic family of proteins mediate

96 11 known channel proteins called aquaporins (AQPs) that are involved in transcellular water transport

97 lled by membrane proteins called aquaporins (AQPs).

98 tes express water channels (i.e. aquaporins (AQPs)), proteins that are increasingly recognized as imp

99 A role for aquaporins (AQPs) in hearing has been suggested from the specific ex

100 All characterized mammalian aquaporins (AQPs) are localized to plasma membranes where they funct

101 standing of the cellular role of aquaporins (AQPs) in the regulation of whole-plant hydraulics, in ge

102 e the expression and function of Aquaporins (AQPs), a family of membrane channel proteins, involved i

103 and pathophysiological roles of aquaporins (AQPs), a family of water channel proteins, in the hepato

104 Compared to other aquaporins (AQPs), lens-specific AQP0 is a poor water channel, and i

105 While overexpression of several aquaporins (AQPs) has been reported in different types of human canc

106 The aquaporins (AQPs) are a family of water-transporting proteins that f

107 vel in the fly renal tissue: the aquaporins (AQPs) Drip and Prip and the aquaglyceroporins Eglp2 and

108 at 65 h (5.1) were significantly affected by AQP deletion.

109 amiloride, but clearance was not affected by AQP deletion.

110 ased from 3.7 to 7.5 but was not affected by AQP deletion.

111 by protein-lipid interactions as modified by AQP-0 homo-oligomerization.

112 he structural requirements rendering certain AQPs permeable for weak monoacids and the mechanism of c

113 of the mercurial-insensitive water channel (AQP-4) at the basolateral membrane of airway epithelial

114 value that is lower than other characterized AQPs.

115 homology to human MIWC (AQP-4, 34%), CHIP28 (AQP-1, 38%), and GLIP (AQP-3, 22%).

116 In conclusion, AQP-2 urinary excretion, as measured by quantitative Wes

117 Under normal conditions, AQP-2 levels in the immature rat were significantly lowe

118 Monocarboxylic acid-conducting AQPs thus employ a mechanism similar to the family of fo

119 g the seven human aquaporins cloned to date (AQPs 0-6), genes encoding the four most closely related

120 ver, direct functional studies demonstrating AQP-mediated water transport in cholangiocytes are limit

121 establish the structural basis for different AQP folding pathways and provide evidence that variation

122 Differential AQP-1 levels affect neural crest cell speed and directio

123 Yet, data on D. discoideum AQPs is almost absent.

124 espite cloning of two putative D. discoideum AQPs, WacA, and AqpA, water permeability has not been sh

125 Thus, glucose-driven, AQP-mediated localized water influx is involved in the m

126 tion assays demonstrated that the endogenous AQP-2 promoter was occupied by TAp73 in a developmentall

127 ic fibrosis (non-CF) or CF airway epithelia, AQP-transfected Fisher rat thyroid cells, or intact lung

128 ting lead cranial neural crest cells express AQP-1 mRNA and protein, implicating a biological role fo

129 with AQP3- or AQP4-encoding genes to express AQPs in plasma membranes.

130 A sequences were designed to target all five AQP genes from the PLASMA MEMBRANE-INTRINSIC PROTEIN1 (P

131 Our results provide evidence for AQP-facilitated tumor cell migration and spread, suggest

132 and spread, suggesting a novel function for AQP expression in high-grade tumors.

133 time a physiologically significant role for AQP-0 in retinal function.

134 We identified and cloned four AQPs from C. lectularius, assessed tissue and lifestage-

135 ctivities that add and remove 32P label from AQP-2.

136 Furthermore, AQP-1 enhances matrix metalloprotease activity and coloc

137 GFP-AQP lateral mobility was measured by irreversibly bleach

138 Using GFP-AQP fusion proteins expressed in HEK293 cells, we demons

139 (AQP-4, 34%), CHIP28 (AQP-1, 38%), and GLIP (AQP-3, 22%).

140 ion bears on the proposed mechanisms for how AQPs remain totally insulating to any proton conductance

141 Using an antibody directed to human AQP-2, a quantitative Western blot analysis was performe

142 bout the development of an antibody to human AQP-2, and measures the urinary excretion of AQP-2 by qu

143 specific as well as glycerol-permeable human AQPs.

144 To examine this hypothesis, AQP genes were silenced using artificial microRNAs that

145 To rule out a potential alteration in AQP-2 trafficking, the transport of this water channel w

146 alone was associated with a mild decrease in AQP-2 protein level in the renal medulla of heterozygous

147 Interestingly, the increase in AQP-2 observed in the immature kidney was not accompanie

148 cellular loop D domain, a region involved in AQP channel gating.

149 wild-type versus knock-out mice deficient in AQPs -1, -4, or -8.

150 and retrieval of "flux proteins", including AQPs, involved in canalicular bile secretion.

151 The hypothesis was tested that the increased AQP expression is associated with increased osmotic wate

152 tration in lithium-induced NDI by increasing AQP-2 expression in the collecting duct principal cells.

153 Instead, AQP-2 phosphorylation by PKA may modulate AQP-2's distri

154 be correlated with downregulation of kidney AQP-2 expression during escape from antidiuresis.

155 fluid volume expansion, also regulate kidney AQP-2 expression in rats.

156 Whole kidney AQP-2 protein was measured by Western blotting, and inne

157 lial cells expressing individual airway/lung AQPs, and perfused mouse lung.

158 Similar to mammalian AQPs, water permeation of AgAQP1 is inhibited by HgCl(2)

159 sured by Western blotting, and inner medulla AQP-2 mRNA was determined by Northern blotting.

160 as a phenomenon in some plant and microbial AQPs.

161 2 (52%), with lesser homology to human MIWC (AQP-4, 34%), CHIP28 (AQP-1, 38%), and GLIP (AQP-3, 22%).

162 e mercurial insensitive water channel (MIWC, AQP-4) is a water-selective transporter expressed at the

163 d, AQP-2 phosphorylation by PKA may modulate AQP-2's distribution between plasma membrane and intrace

164 ndicate that nodulin 26 is a multifunctional AQP that confers water and glycerol transport to the SM,

165 ese novel observations suggest that multiple AQP expression may be advantageous to tumorigenesis, whi

166 We propose that AQPxlo is a new AQP paralogue unknown in mammals.

167 We cloned a novel AQP, AqpB, from amoeboidal D. discoideum cells.

168 The immune-related activity of AQP was confirmed in vivo in a model of acute lung infla

169 Mice lacking functional alleles of AQP-0 had a phototransduction deficit as assessed by ele

170 ole for PKA phosphorylation in alteration of AQP-2's Pf.

171 d immunizing peptide to derive the amount of AQP-2 contained in aliquots of urine.

172 Colocalization of AQP-1 with EphB guidance receptors in the same migrating

173 cally examined the functional consequence of AQP expression in mitochondria by measurement of water a

174 Finally, we discuss how the discovery of AQP activators and inhibitors will be the next major ste

175 omains can play a role in the disposition of AQP-0 and the connexins in the plane of the membrane.

176 osmolalities, significant downregulation of AQP-2 expression compared to dDAVP-infused control rats

177 olality contributes to the downregulation of AQP-2 expression in this model.

178 AQP-2, and measures the urinary excretion of AQP-2 by quantitative Western analysis.

179 Steady-state 24-h urinary excretion of AQP-2 was 43 +/- 10 nmol/24 h (or 28.5 +/- 6.9 pmol/mg c

180 Our findings reveal a new function of AQP in the inflammatory process and suggest a novel ther

181 Inhibition of AQP specifically during the regulatory volume decrease p

182 ished high dDAVP-stimulated kidney levels of AQP-2 after 4 d of water restriction.

183 yers showed that the microdomain location of AQP-0 depended on protein/lipid ratio.

184 ssociated with osmotic water permeability of AQP-expressing cells and a slow phase time constant asso

185 To test whether PKA phosphorylation of AQP-2 alters channel Pf, we compared the Pf values of pu

186 uring maturation a part of the total pool of AQP-1 is differentially sorted and released via the exos

187 The presence of AQP-0 in retinal bipolar cells was also demonstrated, wh

188 e discovery of its role in the regulation of AQP translocation has ramifications for diverse physiolo

189 To study the regulation of AQP-2, immature and adult rats were kept on ad libitum i

190 me inhibitor, MG132, suppresses secretion of AQP-1, implying that ubiquitination is a sorting signal

191 tonicity in vitro regulates the secretion of AQP-1, thus showing that extracellular osmotic condition

192 ane, whereas water loading caused a shift of AQP-2 channels back to intracellular vesicles in both ad

193 A subgroup of AQP water channels also facilitates transmembrane diffus

194 5) cm3/s, a value 30-fold lower than that of AQP 1, the red blood cell water channel.

195 ration and dDAVP stimulated translocation of AQP-2 from intracellular vesicles to the plasma membrane

196 based on a comparative mutagenic analysis of AQPs 1, 3, and 4, suggest that loop D interactions may p

197 colorectal carcinogenesis, the expression of AQPs 1 and 5 was induced in early-stage disease (early d

198 Expression of AQPs 1 and 5 was maintained even in metastatic lesions i

199 is showed that tissue-specific expression of AQPs 1, 2, 3, and 5 was not affected by AQP4 deletion.

200 erase chain reaction analysis, expression of AQPs 1, 3, and 5 was found in seven colon and colorectal

201 NAs to specifically reduce the expression of AQPs in epithelial cells and provides direct evidence of

202 he performances and the natural functions of AQPs.

203 unofluorescence suggested the involvement of AQPs 3, 4, and 5 in high airway water permeability.

204 Based on the involvement of AQPs in angiogenesis and cell migration, we tested wheth

205 /s, which is within 2 orders of magnitude of AQPs' rates, and reject all ions except protons.

206 on the function and regulation mechanism of AQPs.

207 An alternative is controlling the number of AQPs in the cell membrane.

208 different types of human cancer, the role of AQPs in carcinogenesis has not been clearly defined.

209 However, clarifying the role of AQPs in mediating water transport in biliary epithelia h

210 The role of AQPs in the hierarchy of the hydraulic signal pathway co

211 ealing and also explain the pivotal roles of AQPs in carcinogenesis and metastasis.

212 ut the effects of a hypotonic environment on AQP abundance.

213 The transcriptional effects of TAp73 on AQP-2 and B2R were independent of p53.

214 number of phytocompounds and their action on AQPs and related potential clinical achievements.

215 beneficial effect of such phytocompounds on AQPs in health and disease is a matter of intensive rese

216 Importantly, only AQP and three novel DEGs associated with stress, mangane

217 Only AQPs exhibited significant osmotic water permeability (P

218 mercurial-insensitive water channel (MIWC or AQP-4) is a 30-32 kDA integral membrane protein expresse

219 al and pathological roles of these and other AQP isoforms.

220 reduced water conductance relative to other AQPs.

221 cm/s) of endosomes containing phosphorylated AQP-2 (0.7 +/- 0.3 mol of PO4/mol of protein) is not sig

222 sights into the translational value of plant AQPs.

223 els of the astrocytic water channel protein, AQP-4, and matrix metalloprotease, MMP-9.

224 Analysis of purified AQP-0 reconstituted in raft-containing bilayers showed t

225 el Pf, we compared the Pf values of purified AQP-2 endosomes after incubation with either PKA or alka

226 les gambiae contains at least seven putative AQP sequences.

227 dehydration that was accompanied by reduced AQP-2 protein expression.

228 on the regulation of the expression of renal AQP and NKCC2, studies were performed with hyperosmolali

229 n and prevented the down-regulation of renal AQP-2 protein expression in mice with lithium-induced ne

230 Hydrogen sulfide upregulates renal AQP-2 protein expression and promotes urine concentratio

231 is not significantly different from the same AQP-2 endosomes where 95 +/- 8% of the phosphate has bee

232 P) in cellular water permeability and screen AQP-specific drugs.

233 highly water and neutral substrate selective AQP family.

234 he case in the structures of water-selective AQPs AqpZ and AQP1, the asparagines of the 2 Asn-Pro-Ala

235 porins (AQP0-AQP12) cloned in mammals, seven AQPs have been identified in the liver and biliary tree.

236 s demonstrate that the expression of several AQPs is found in tumor cells and is associated with an e

237 Increased expression of several AQPs, especially AQP5, is associated with increased canc

238 de evidence against functionally significant AQP expression in mitochondria, which is consistent with

239 ted, in part because of the lack of specific AQP inhibitors.

240 We identified an efficient water-specific AQP (ClAQP1), two aquaglyceroporins (ClGlp1 and ClGlp2)

241 al when co-expressed with the water-specific AQP.

242 Quantification of baseline steady-state AQP-2 excretion was done by collecting urine on the day

243 Furthermore TAp73 stimulated AQP-2 promoter-driven reporter expression.

244 In the second study, AQP-2 expression was evaluated in different regions of k

245 In summary, AQP-2 expression and trafficking in the immature kidney

246 annel conductance that is 30-fold lower than AQP 1.

247 We conclude that AQP-mediated water transport in macrophages constitutes

248 Results demonstrate that AQP-1 is up-regulated in the small, angiogenic, neovascu

249 f this study was to test the hypothesis that AQP-1 is involved in amoeboid motility and angiogenic in

250 serum-responsive gene, we hypothesized that AQP expression may be involved in the development of hum

251 Taken together these results indicate that AQP-0 and connexins can be segregated in the membrane by

252 Studies using [gamma-32P]ATP reveal that AQP-2 endosomes contain endogenous PKA and phosphatase a

253 where freeze-fracture experiments show that AQP-0 oligomerizes (3).

254 Here, we show that AQP-1 is partially lost during in vitro maturation of mo

255 ults, confocal microscopy images showed that AQP-0 was sequestered into raft microdomains in the 1:10

256 These results lead us to suggest that AQP-1 sorting into exosomes may be the mechanism by whic

257 Our data demonstrate that AQPs have critical roles in excretion, water homeostasis

258 It was hypothesized that AQPs contribute to cell elongation processes by allowing

259 sed to water-related stress, suggesting that AQPs may offer novel control avenues.

260 Accumulating evidence suggests that AQPs are likely involved in canalicular and ductal bile

261 The AQP-expressing cells showed at least 10x faster osmotic

262 mosome locus 12q13, the same location as the AQP.2 and MIP genes.

263 pH did not differ significantly in the AQP knockout mice.

264 AQP3 is the AQP that is expressed in the skin where it facilitates c

265 hallmarks of the water-selective arm of the AQP family of proteins.

266 e been observed for different members of the AQP family, the signature homotetrameric quaternary stru

267 Unlike other members of the AQP family, the unique distribution in intracellular mem

268 oocytes with very low concentrations of the AQP inhibitors HgCl(2) and AgNO(3).

269 of Xenopus oocytes expressing members of the AQP or Rh family.

270 ents the hypothesis that measurements of the AQP-2 excretion rate might be used as a marker of collec

271 shown to depend on water-wires spanning the AQP pore that reverse their orientation, combined with c

272 anism of selective water passage through the AQP pore are established, but there remains a gap in our

273 controlling the passage of water through the AQP pore, but this only has been observed as a phenomeno

274 framework for tetrameric assembly within the AQP family.

275 s mediated by the cytoplasmic domains of the AQPs.

276 This shows that the AQPs have dual functions in epithelial physiology: as ch

277 We hypothesized that the AQPs of the vascular bundle sheath (BS) cells regulate K

278 that will facilitate the evaluation of these AQP isoforms as drug targets.

279 Another fact is that the expression of these AQPs is highly modulated by the external osmolality.

280 f the chloride shunt conductance, with these AQPs localizing to opposite plasma membranes.

281 this model, we define the importance of this AQP in water transport across biliary epithelia.

282 ed to acquire a better understanding of this AQP subfamily.

283 Some TIP-AQP genes, such as TIP2;2 and TIP1;1, are up-regulated u

284 s called tonoplast-intrinsic aquaporins (TIP-AQPs).

285 We propose that TIP-AQPs affect the induction of leaf abscisic acid, which l

286 ificant defect, yet to be defined, distal to AQP-2.

287 water channels with 2.6 A pores, similar to AQP channels, that encapsulate oriented dipolar water-wi

288 rin 1 (AgAQP1) protein that is homologous to AQPs known in humans, Drosophila, and sap-sucking insect

289 ted exhibited water permeability, similar to AQPs, although accompanied by a proton conductance.

290 sis thaliana), the highly abundant tonoplast AQP isoforms AtTIP1;1, AtTIP1;2, and AtTIP2;1 facilitate

291 r the isotype control, failed to translocate AQP to the plasma membrane.

292 H(2)S upregulated AQP-2 protein expression, probably via the cAMP-PKA path

293 Urinary AQP-2 excretion correlated best with solute-free water c

294 Compared to placebo, urinary AQP-2 excretion decreased significantly and in all group

295 Thereafter, urinary AQP-2 excretion was calculated as a ratio of the urine f

296 Therefore, urinary AQP-2 excretion can be quantified by using Western analy

297 (2+) and Ni (2+) on the water transport via AQPs.

298 odium (SCNN1-B, ATP1-A1, ATP1-B1) and water (AQP-4) movement in the fetal lung.

299 nt soluble membrane (DSM) fractions, whereas AQP-0 was found in both detergent resistant membrane and

300 enesis and cell migration, we tested whether AQP expression in tumor cells might enhance their migrat