ライフサイエンスコーパス: AQP6

1 corroborated that amino acids present within AQP6(194-213) of AQP6 loop C contribute to intracellular

2 length 5'-AQP2-5 kb spacer-AQP5-7 kb spacer-AQP6-3'.

3 AQP6 colocalizes with H+-ATPase in intracellular vesicle

4 When expressed in Xenopus laevis oocytes, AQP6 exhibits low basal water permeability; however, whe

5 Unlike other aquaporins, AQP6 functions not as a water channel but as an anion-se

6 is indicated the presence of AQP2, AQP5, and AQP6 genes, but not AQP0.

7 Uniquely, all known mammalian orthologs of AQP6 have an asparagine residue (Asn-60) at the position

8 ly related aquaporins (AQP0, AQP2, AQP5, and AQP6) have been mapped to chromosome band 12q13, suggest

9 g a polyclonal antibody to the C terminus of AQP6, immunoblots revealed a major 30-kDa band in membra

10 afficking and severely curtail expression of AQP6 in heterologous expression systems.

11 AQP2, AQP3, and AQP4 in the collecting duct; AQP6 in the papilla; and AQP7 in the proximal tubule.

12 The inability to over-express Aquaporin 6 (AQP6) in the plasma membrane of heterologous cells has h

13 In proximal tubules, AQP6 is also abundant in membrane vesicles within the su

14 Here we show that AQP6 is functionally distinct from other known aquaporin

15 Here we show that AQP6 is localized exclusively in intracellular membranes

16 n microscopic studies confirmed that >98% of AQP6 is located in intracellular membrane vesicles.

17 iple types of renal epithelia indicates that AQP6 is not simply involved in transcellular fluid absor

18 In glomeruli, AQP6 is present in membrane vesicles within podocyte cel

19 ieved to be located in plasma membranes, rat AQP6 is restricted to intracellular vesicles in renal ep

20 amino acids present within AQP6(194-213) of AQP6 loop C contribute to intracellular endoplasmic reti

21 at the contact point between TM2 and TM5 in AQP6 may function as a teeter board needed for rapid str

22 l inhibitor, Hg2+, the water permeability of AQP6 oocytes rapidly rises up to tenfold and is accompan

23 tance is rapidly and reversibly activated in AQP6 oocytes.

24 Moreover, our studies predict that AQP6 participates in distinct physiological functions su

25 In collecting duct, AQP6 resides in intracellular membrane vesicles in apica

26 ion of rat kidney homogenates confirmed that AQP6 resides predominantly in vesicular fractions, and i

27 we have identified a region within loop C of AQP6 that is responsible for severely hampering plasma m

28 Single-channel analyses have shown AQP6 to flicker rapidly between closed and open status.

29 t a single residue substitution (N60G in rat AQP6) totally eliminates the anion permeability of AQP6

30 totally eliminates the anion permeability of AQP6 when expressed in Xenopus oocytes, but the N60G ooc