ライフサイエンスコーパス: B. abortus

1 B. abortus 2308 persisted within these cathepsin D(-), L

2 B. abortus ahpC and katE mutants exhibit wild-type virul

3 B. abortus and B. abortus DNA-stimulated IL-12 productio

4 B. abortus cell-cycle progression is coordinated with in

5 B. abortus cydB::Tn5 strains exhibited heightened sensit

6 B. abortus given without OVA did not diminish the subseq

7 B. abortus O antigen appears to be essential for extra-

8 B. abortus RA1 was attenuated for survival in mice.

9 B. abortus, B. melitensis, B. suis, and B. canis produce

10 In the absence of IL-10, B. abortus LPS induced strong IFN-gamma responses, but I

11 quence, primers were designed to construct a B. abortus deletion mutant.

12 In the mouse model, a B. abortus virB mutant was initially able to colonize th

13 hypersensitive to UV damage, surprisingly a B. abortus recA null mutant conferred only modest sensit

14 was uncovered when it was determined that a B. abortus dhbC mutant (BHB1) defective in 2,3-DHBA prod

15 acellular replication, the numbers of acidic B. abortus 2308 BCP decreased while remaining cathepsin

16 strain RB51 or its vaccine efficacy against B. abortus 2308 challenge.

17 cinated cattle did not contain agglutinating B. abortus antibody in the tube agglutination test for b

18 al concentration of hydrogen peroxide allows B. abortus to adapt so as to survive subsequent exposure

19 Although B. abortus-activated T cells actively secreted the pro-o

20 Although B. abortus-infected glial cells secreted IL-1beta and TN

21 enes, we searched the Brucella suis 1330 and B. abortus 2308 genomes for genes with an upstream virB

22 smooth, virulent Brucella melitensis 16M and B. abortus 2308, rough wboA mutants VTRM1, RA1, and WRR5

23 However, only B. abortus and B. abortus DNA induced high levels of IFN-gamma and IL-1

24 Th1-like cytokine response to B. abortus and B. abortus DNA, which was confirmed by using neutralizin

25 B. abortus and B. abortus DNA-stimulated IL-12 production was maximal b

26 strate that the cross talk of LX-2 cells and B. abortus induces autophagy and fibrosis with concomita

27 The lipid A molecules of S. meliloti and B. abortus are unusually modified with a very-long-chain

28 Since both the S. meliloti and B. abortus bacA mutants have an increased resistance to

29 he chronic infection of both S. meliloti and B. abortus.

30 region should include both B. melitensis and B. abortus.

31 bacterial pathogens Brucella melitensis and B. abortus.

32 itensis isolated from seven participants and B. abortus from one.

33 rain S19, and attenuated mutants S19vjbR and B. abortus DeltavirB2 While B. abortus 2308 and S19 repl

34 tructures of the enzyme from B. subtilis and B. abortus and describe the C teminus structure which ac

35 as B. abortus strain RB51, 57 identified as B. abortus biovar 1, 15 identified as B. abortus bv.2, 1

36 entified as B. abortus bv.2, 1 identified as B. abortus bv.2 (M antigen dominant), 7 identified as B.

37 ied as B. abortus biovar 1, 15 identified as B. abortus bv.2, 1 identified as B. abortus bv.2 (M anti

38 s bv.2 (M antigen dominant), 7 identified as B. abortus bv.4, and 22 identified as B. abortus S2308 a

39 This included 120 isolates identified as B. abortus S19, 9 identified as B. abortus strain RB51,

40 ied as B. abortus bv.4, and 22 identified as B. abortus S2308 and isolated from experimentally infect

41 dentified as B. abortus S19, 9 identified as B. abortus strain RB51, 57 identified as B. abortus biov

42 -cytotoxic to human host cells and attenuate B. abortus replication in the intracellular niche.

43 To identify in vivo interactions between B. abortus and the host that lead to persistent infectio

44 th stress survival and the interface between B. abortus and the host immune system.

45 rium tumefaciens is a close relative of both B. abortus and S. meliloti, and this bacterium is the ca

46 dozen genes, the genetic complements of both B. abortus strains are identical, whereas the three spec

47 Animals infected with both B. abortus and B. pahangi showed increased IFN-gamma and

48 ivation and further secretion of IL-1beta by B. abortus in macrophages.

49 T4SS, we compared host responses elicited by B. abortus with those of an isogenic mutant in the virB

50 indicate that activated T cells, elicited by B. abortus-infected macrophages and influenced by the in

51 chanisms of liver immune response induced by B. abortus-infected hepatic stellate cells.

52 d maintenance of chronic spleen infection by B. abortus in mice, experimental infection of pregnant c

53 Infection of macrophages by B. abortus was stimulated by light in the wild type but

54 to evasion of adaptive immune mechanisms by B. abortus.

55 innate immunity of the CNS set in motion by B. abortus contributes to the activation of the blood-br

56 as restored by an intact copy of cydAB or by B. abortus genes encoding the oxidative radical-scavengi

57 ase-excision repair pathways are required by B. abortus to face this stress in vitro and in a mouse i

58 Signaling pathways triggered by B. abortus involves stimulator of IFN genes (STING), whi

59 U of B. abortus S19 or the vaccine candidate B. abortus S19DeltavjbR and monitored for the developmen

60 Here a highly conserved B. abortus homolog of the R. meliloti bacA gene, which e

61 When used to infect macrophages, DeltaznuA B. abortus showed minimal growth.

62 Further study with DeltaznuA B. abortus showed that its virulence in BALB/c mice was

63 catalase deletion mutations in two different B. abortus strains and used two-dimensional gel analysis

64 pression of proinflammatory cytokines during B. abortus infection.

65 also important for the UPR induction during B. abortus infection.

66 esident transmembrane molecule STING, during B. abortus infection.

67 sed interleukin-12 p40 mRNA following either B. abortus or B. abortus-OVA administration.

68 In extravillous trophoblasts (EVTs), B. abortus and B. suis replicated within single-membrane

69 nant O. anthropi strain 49237SOD, expressing B. abortus Cu,Zn SOD, provided protection against virule

70 addition, priming cells with IFN-beta favors B. abortus survival in macrophages.

71 y, the response of macrophages 4 h following B. abortus infection was analyzed to identify early intr

72 R/C, and Pr phages that are lytic mainly for B. abortus, Brucella melitensis and Brucella suis; Group

73 assay was 800 CFU equivalents/ml of milk for B. abortus and as low as 4 CFU equivalents per ml of mil

74 roteins represent important iron sources for B. abortus 2308 during its residence in the mammalian ho

75 utant, designated MEK2, was constructed from B. abortus 2308 by gene replacement, and the sodC mutant

76 an isogenic ftrA mutant was constructed from B. abortus 2308.

77 EC was dependent on IL-1beta because CS from B. abortus-infected astrocytes and microglia deficient i

78 ysis of an isogenic bhuA mutant derived from B. abortus 2308 verified that there is a link between Bh

79 NHS than OPS-deficient strains derived from B. abortus 2308, (ii) both the classical and the MBL-med

80 l X-ray crystal structure of the enzyme from B. abortus has been solved and refined at 2.7 A resoluti

81 lt from 12 pentameric units, the enzyme from B. abortus is pentameric in its crystalline form.

82 t mRNAs, indicating that the AbcR sRNAs from B. abortus 2308 perform redundant regulatory functions.

83 In contrast, supernatants from B. abortus-infected hepatocytes and monocytes induce MMP

84 . abortus, the capacity of supernatants from B. abortus-infected hepatocytes and monocytes to induce

85 transposon mutagenesis was used to generate B. abortus rough mutants defective in O-antigen presenta

86 In order to understand how B. abortus copes with the conditions during intracellula

87 cking either NADPH oxidase or iNOS, however, B. abortus infected and persisted to the same extent as

88 We identified B. abortus genes involved in chronic infection, by asses

89 In B. abortus 2308, MucR regulates a wide variety of genes

90 Maximum expression of bhuA in B. abortus 2308 is observed during stationary phase when

91 the ccrM gene results in striking changes in B. abortus morphology, DNA replication, and growth in mu

92 olysis is a unique feature of GSR control in B. abortus.

93 Allelic exchange of genomic DNA in B. abortus 2308 mediated by electroporation of pJM6 prod

94 Because CcrM is essential in B. abortus and increased ccrM copy number attenuates sur

95 suggest that only one copy of galE exists in B. abortus 2308.

96 iron needed to repress dhbCEBA expression in B. abortus 2308 was also greater when this strain was cu

97 proteolysis is a novel regulatory feature in B. abortus that ensures proper control of GSR transcript

98 for a conserved gene of unknown function in B. abortus envelope stress resistance and infection.

99 We propose that galE in B. abortus evolved by lateral transfer from other animal

100 product, the expression of the mntH gene in B. abortus 2308 is repressed by Mn(2+), but not Fe(2+),

101 ted AbcR1 and AbcR2) have been identified in B. abortus 2308.

102 Transcription of the ftrA gene is induced in B. abortus 2308 in response to iron deprivation and expo

103 on; its transcription is strongly induced in B. abortus by various stressors encountered by the bacte

104 epair and resistance to oxidative killing in B. abortus 2308, XthA-1 is not required for wild-type vi

105 , the transcriptional start site for mntH in B. abortus 2308 was determined by primer extension analy

106 present study the genes regulated by MucR in B. abortus have been elucidated using microarray analysi

107 A znuA knockout mutation in B. abortus 2308 (DeltaznuA) was constructed and found to

108 detectable levels of Irr were found only in B. abortus 2308 cells by Western blot analysis following

109 hydroperoxide, but not hydrogen peroxide, in B. abortus 2308 and that OhrR represses the transcriptio

110 A protein, DNA repair, and SOS regulation in B. abortus.

111 highlight that the control of DNA repair in B. abortus displays distinct features that are not prese

112 -kDa protein, which was detected in vitro in B. abortus grown to stationary phase.

113 melitensis 16 M would be similar to znuA in B. abortus and questioned whether it may also be an impo

114 Thus, inactivated B. abortus can induce non-CD4+ cells to produce the cyto

115 intracellular bacterial pathogens, including B. abortus Results from this study indicate that adverse

116 nique to the alpha-proteobacteria, including B. abortus.

117 In samples from India, B. abortus shedding was identified in 86% of milk ring t

118 y constitutively and intracellularly induced B. abortus genes.

119 Twenty-six compounds inhibited B. abortus metabolism in axenic culture, thirteen of whi

120 n-cytotoxic compounds specifically inhibited B. abortus replication in the intracellular niche, which

121 Interestingly, B. abortus 2308 impaired the growth of osteoclasts witho

122 An isogenic B. abortus bacA mutant exhibited decreased survival in m

123 Heat-killed B. abortus (HKBA) is a strong Th1 adjuvant and carrier.

124 Recently we demonstrated that heat-killed B. abortus (HKBa), a strong Th1 stimulus, conjugated to

125 hose seen for control particles (heat-killed B. abortus 2308, live Escherichia coli HB101, or latex b

126 set of genes that closely overlaps the known B. abortus GSR regulon.

127 in D(+) BCP was significantly lower for live B. abortus 2308-infected cells than for either Brucella

128 As a result of this finding, a new B. abortus htrA mutant, designated RWP11, was constructe

129 conjugated to ovalbumin (HKBA-OVA), but not B. abortus alone, can alter the antigen-specific cytokin

130 Furthermore, B. melitensis but not B. abortus nor B. suis interfered with the invasive capa

131 y of 480 known bioactive compounds for novel B. abortus anti-infectives.

132 etion of virB7 did not reduce the ability of B. abortus to persist in spleens of mice.

133 0, or virB11 markedly reduced the ability of B. abortus to persist in the spleens of mice at 8 weeks

134 he virB locus markedly reduce the ability of B. abortus to survive in cultured macrophages or to pers

135 All mutations reduced the ability of B. abortus to survive in J774A.1 mouse macrophage-like c

136 This proteomic analysis of B. abortus provides novel insights into the mechanisms u

137 in nasal swabs (2.9%) and milk (1.4%) and of B. abortus in milk (11.4%).

138 ntrols the cellular and infection biology of B. abortus.

139 of 1 x 10(4), 1 x 10(5), or 1 x 10(6) CFU of B. abortus S19 or the vaccine candidate B. abortus S19De

140 revealed several deletions on chromosomes of B. abortus and B. melitensis that encoded proteins of va

141 unctions of Igh6(-/-) mice in the context of B. abortus infection.

142 Thus, proper control of B. abortus division site polarity is necessary for survi

143 dition of erythritol to low-iron cultures of B. abortus 2308 stimulated the production of 2,3-DHBA by

144 Here, we study the cell cycle of B. abortus at the single-cell level, in culture and duri

145 s to OVA/alum is secondary to the effects of B. abortus on expression of costimulatory molecules on T

146 Early trafficking events of B. abortus 2308-containing phagosomes (BCP) were indisti

147 fic targets (a region of the BCSP31K gene of B. abortus and a repeat-sequence region in the hsp65 gen

148 Here we present the genome of B. abortus 2308, the virulent prototype biovar 1 strain,

149 Identification of B. abortus genes that are activated following macrophage

150 A 640-kb inversion on chromosome II of B. abortus has been reported previously and is further d

151 efficacy of these molecules as inhibitors of B. abortus replication in the intracellular niche sugges

152 ek-old mice were given a single injection of B. abortus in the absence or presence of OVA, and at mat

153 e study the ability of a single injection of B. abortus to switch a Th2 to a Th1 response in immature

154 in parenchyma upon intracranial injection of B. abortus was diminished in the absence of Nod-like rec

155 ntrast, mice receiving a single injection of B. abortus-OVA at the age of 1 week, but not those injec

156 Transposon (Tn5) mutagenesis of B. abortus and the subsequent screening of mutants for s

157 the htrA gene product to the pathogenesis of B. abortus infections.

158 ent infection, we studied the persistence of B. abortus and an isogenic virB mutant deficient in the

159 ement for the T4SS to mediate persistence of B. abortus in the spleen.

160 by PPARgamma promotes chronic persistence of B. abortus within AAMs, and targeting this pathway may a

161 was also dispensable for the persistence of B. abortus, B. melitensis, and B. suis in mice up to 4 w

162 The in vitro phenotype of B. abortus RWP11 is consistent with the proposed functio

163 plays an important role in the physiology of B. abortus 2308, particularly during its intracellular s

164 with OVA/alum in the absence or presence of B. abortus.

165 Cu/Zn superoxide dismutase (SOD) protein of B. abortus in strain RB51.

166 findings indicate that the 14-kDa protein of B. abortus possesses lectin-like properties and is essen

167 A 14-kDa protein of B. abortus was previously identified to be immunogenic i

168 We demonstrate recognition of B. abortus antibodies through capture by fluorescent sil

169 ake was crucial for increased replication of B. abortus in AAMs, and for chronic infection, as inacti

170 overexpression of a protective antigen(s) of B. abortus in strain RB51 should enhance its vaccine eff

171 The galE sequence of B. abortus 2308 is more similar to galE from other anima

172 The complete genomic sequence of B. abortus provides an important resource for further in

173 A high prevalence of shedding of B. abortus (samples from India) and M. bovis (samples fr

174 fection rates in animals similar to those of B. abortus.

175 0, and virB11 are essential for virulence of B. abortus in mice, while functions encoded by the virB1

176 thought to be important to the virulence of B. abortus in pregnant ruminants.

177 h several fragments were shared between only B. abortus and B. suis, B. abortus shared more fragments

178 However, only B. abortus and B. abortus DNA induced high levels of IFN

179 Thus, opsonized B. abortus in human monocytes survives within phagosomes

180 n-12 p40 mRNA following either B. abortus or B. abortus-OVA administration.

181 xperimentally infected with B. melitensis or B. abortus and monitored for 24 weeks.

182 response to iron deprivation in the parental B. abortus 2308 strain, and a direct and specific intera

183 R. meliloti, and the intracellular pathogen B. abortus showed that this sequence conservation extend

184 e, LovhK (bab2_0652), functions as a primary B. abortus GSR sensor.

185 ile Ohr plays a prominent role in protecting B. abortus 2308 from organic hydroperoxide stress in in

186 hA-1 gene product participates in protecting B. abortus 2308 from oxidative damage.

187 imental findings indicate that SodC protects B. abortus 2308 from the respiratory burst of host macro

188 hibitors of intracellular replication reduce B. abortus metabolism in axenic culture and perturb feat

189 conserved signalling pathway that regulates B. abortus stress physiology and infection biology.

190 nase functions as a photoreceptor regulating B. abortus virulence.

191 Rough B. abortus mutants were more sensitive to the bactericid

192 rmed upon three pathogenic Brucella species: B. abortus, B. melitensis, and B. suis.

193 w the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.

194 d with those of the virulent parental strain B. abortus 2308.

195 triction in comparison to its parent strain, B. abortus 2308, when cultured in the presence of erythr

196 utant BEA5, and, unlike the parental strain, B. abortus BEA5 cannot utilize heme as an iron source in

197 and S708 phages that are lytic for B. suis, B. abortus and B. neotomae.

198 shared between only B. abortus and B. suis, B. abortus shared more fragments and had fewer nucleotid

199 Surprisingly, B. abortus induced down-regulation of CD28 and up-regula

200 We conclude that B. abortus stimulates a cellular as well as a humoral im

201 We demonstrated that B. abortus infection induces the expression of the UPR t

202 t functional analyses that demonstrated that B. abortus RadA complements a radA defect in E. coli but

203 These findings indicate that B. abortus strain RB51 vaccination of mice induces speci

204 nsertions supports previous indications that B. abortus and B. melitensis share a common ancestor tha

205 We considered the possibility that B. abortus RadA might be compensating for the loss of Re

206 The results reveal that B. abortus causes a chronic infection of lung tissue in

207 These studies show that B. abortus strains that do not express catalase activity

208 resents a relevant environmental stress that B. abortus 2308 must deal with during its residence in t

209 The results of these studies suggest that B. abortus 2308 has at least one other heme oxygenase th

210 In conclusion, these data suggest that B. abortus can alter macrophage pathways to recruit addi

211 Finally, these results suggest that B. abortus-induced UPR is triggered by bacterial cyclic

212 e B. abortus dhbCEBA operon, suggesting that B. abortus has the genetic capacity to produce a more co

213 nly for long-term persistence, suggests that B. abortus uses distinct sets of virulence determinants

214 The B. abortus bab1_1517 mutant strain was significantly att

215 The B. abortus bhuA mutant HR1703 exhibits significant atten

216 The B. abortus ftrA mutant exhibits significant attenuation

217 The B. abortus gal E mutant is not altered in colony morphol

218 The B. abortus GSR signaling pathway has multiple layers of

219 The B. abortus mntH mutant also exhibits extreme attenuation

220 The B. abortus mntH mutant MWV15 exhibits increased suscepti

221 The B. abortus sodC mutant was also found to be much more se

222 The B. abortus xthA-1 mutant also displayed reduced resistan

223 Although the B. abortus xthA-1 mutant exhibited increased sensitivity

224 ved from these two Brucella species, and the B. abortus vaccine strain RB51 was assayed using normal

225 irulence in C57BL/6 and BALB/c mice, but the B. abortus ahpC katE double mutant is extremely attenuat

226 ntribute to the attenuation displayed by the B. abortus hfq mutant Hfq3 in the mouse model.

227 The attenuation displayed by the B. abortus sodC mutant in both resting and IFN-gamma-act

228 ichia coli mutants were used to identify the B. abortus genes (designated dhbC, -B, and -A) responsib

229 B. suis or B. melitensis were present in the B. abortus genome.

230 s was not the only mechanism involved in the B. abortus-mediated inhibition of the Th2 response to OV

231 Introduction of a bhuQ mutation into the B. abortus dhbC mutant BHB2 (which cannot produce sidero

232 killing by cultured murine macrophages, the B. abortus sodC mutant also displayed significant attenu

233 sible explanation for the attenuation of the B. abortus 2,3-DHBA-deficient mutant BHB1 in pregnant ru

234 parison of the growth characteristics of the B. abortus bhuA mutant and 2308 in this medium suggested

235 defect, suggesting that the inability of the B. abortus dhbC mutant to display wild-type growth in th

236 on analysis showed that transcription of the B. abortus dhbCEBA operon originates from two iron-regul

237 in-frame deletion of 660 bp with galE of the B. abortus genome by marker exchange.

238 A genome-scale analysis of the B. abortus GSR regulon identified stress response genes

239 The inability of the B. abortus hfq mutant to survive and replicate in a wild

240 in E. coli but could not act in place of the B. abortus RecA.

241 All of the B. abortus rough mutants exhibited a 4- to 5-log-unit in

242 rain 2308, and unlike its parent strain, the B. abortus bhuA mutant is unable to maintain a chronic s

243 Compared with the parental strain, the B. abortus ftrA mutant displays a decreased capacity to

244 d in mice spleens for a longer time than the B. abortus vaccine strain RB51, but as expected, neither

245 We conclude that the B. abortus GSR system regulates acute stress adaptation

246 Thus, the B. abortus GSR system is dispensable for colonization bu

247 nscription product of this gene binds to the B. abortus catalase promoter region.

248 Restoring 2,3-DHBA production to the B. abortus dhbC mutant by genetic complementation abolis

249 flanking regions immediately adjacent to the B. abortus dhbCEBA operon, suggesting that B. abortus ha

250 mental infection of pregnant cattle with the B. abortus dhbC mutant BHB1 clearly showed that producti

251 Mice infected with the B. abortus virB mutant elicited smaller increases in ser

252 the spleen during a mixed infection with the B. abortus wild type.

253 This B. abortus gene has been named wboA.

254 However, with time, B. abortus infection induced Beclin-1 cleavage with conc

255 her human pathogenic Brucella species and to B. abortus field isolate 9-941.

256 Conjugation to B. abortus was required for in vivo priming, since there

257 In contrast to B. abortus 2308, the isogenic hfq and bacA mutants remai

258 AIM2, and AIM2 KO mice are less resistant to B. abortus infection.

259 wnmodulate the Th1-like cytokine response to B. abortus and B. abortus DNA, which was confirmed by us

260 a coli LPS and DNA mirrored the responses to B. abortus components, suggesting that immune effects ob

261 ase-1 knockout mice were more susceptible to B. abortus infection than were wild-type animals, sugges

262 vaccine, since protection against wild-type B. abortus 2308 challenge was as effective as that obtai

263 ges in vitro increased recovery of wild-type B. abortus but not recovery of a virB mutant.

264 were higher in mice infected with wild-type B. abortus than in mice infected with the virB mutant, a

265 une response in mice infected with wild-type B. abortus than in mice infected with the virB mutant.

266 t in spleens of mice infected with wild-type B. abortus than in virB mutant-infected mice.

267 oad-host-range plasmid bearing the wild-type B. abortus wboA gene resulted in the restoration of O-si

268 -12p40 than did mice infected with wild-type B. abortus.

269 Unlike B. abortus, both smooth and rough strains of B. melitens

270 B. neotomae is a rodent pathogen, and unlike B. abortus, B. melitensis, and B. suis, B. neotomae has

271 cloned DHBA biosynthesis locus from virulent B. abortus 2308 and genetic complementation of defined E

272 gene encoding the 14-kDa protein in virulent B. abortus strain 2308 induced a rough-like phenotype wi

273 ndosomal pathway and replication of virulent B. abortus 2308 within these vesicles corresponds with a

274 ruption of the wboA gene in smooth, virulent B. abortus, Brucella melitensis, and Brucella suis resul

275 f protection against challenge with virulent B. abortus strain 2308 or B. suis strain 1330 but no pro

276 e there was no induction of memory CTLs when B. abortus was only mixed with peptide.

277 ants S19vjbR and B. abortus DeltavirB2 While B. abortus 2308 and S19 replicated inside mature osteocl

278 ated, murine spleen cells were cultured with B. abortus derived DNA, lipopolysaccharide (LPS), or who

279 ith Brucella abortus Infection of HBMEC with B. abortus induced the secretion of IL-6, IL-8, and MCP-

280 on-T and T cells following immunization with B. abortus.

281 bserved in the spleens of mice infected with B. abortus 2308 or a virB mutant.

282 Mice infected with B. abortus 2308 produced an antibody response to the pro

283 ed into mature osteoclasts and infected with B. abortus 2308, the vaccine strain S19, and attenuated

284 lls (astrocytes and microglia) infected with B. abortus also induced activation of HBMEC, but to a gr

285 Livers from mice infected with B. abortus displayed a fibrogenic phenotype with patches

286 e supernatant from macrophages infected with B. abortus induced bone marrow-derived monocytes (BMMs)

287 ve activity, while osteoclasts infected with B. abortus S19 and S19vjbR were significantly larger and

288 Yet, if LX-2 cells are infected with B. abortus, the capacity of supernatants from B. abortus

289 s from lemmings experimentally infected with B. abortus.

290 protein indicated that mice inoculated with B. abortus 19 or 2308 or B. melitensis RM1 also produced

291 tails were observed in mice inoculated with B. abortus S19 but not in those inoculated with S19Delta

292 In contrast, mice inoculated with B. abortus S19DeltavjbR did not show significant bone ch

293 of macrophages or osteoclast precursors with B. abortus 2308 resulted in generation of smaller osteoc

294 Furthermore, macrophages preinfected with B. abortus S2308 or pretreated with B. abortus O polysac

295 ted with B. abortus S2308 or pretreated with B. abortus O polysaccharide did not prevent rough CA180-

296 ta suggest that the prior sensitization with B. abortus may induce a down regulation of the Th2 respo

297 cold-fixation complement fixation tests with B. abortus and B. melitensis antigens (CFA and CFM), USD

298 estern blots, sera from mice vaccinated with B. abortus RB51 recognized YajC but not SecD.

299 otein, splenocytes from mice vaccinated with B. abortus RB51 were able to proliferate and produce gam

300 antibodies from BALB/c mice vaccinated with B. abortus RB51.