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1 B. cenocepacia (genomovar III) is the most prevalent and
2 B. cenocepacia can survive intracellularly in macrophage
3 B. cenocepacia efficiently activates the inflammasome an
4 B. cenocepacia employs a type VI secretion system (T6SS)
5 B. cenocepacia strain J2315 was isolated from a CF patie
6 B. cenocepacia zmpB and zmpA zmpB mutants had no proteol
7 ution (BMD), and MicroScan WalkAway using 50 B. cenocepacia and 50 B. multivorans isolates collected
8 R-based structural analyses, we identified a B. cenocepacia O-glycosylation (ogc) gene cluster necess
12 to these responses, the interaction between B. cenocepacia and Toll-like receptor 5 (TLR5) was inves
13 ndent production of secondary metabolites by B. cenocepacia strains and suggests future work which co
14 demonstrate that production of ornibactin by B. cenocepacia in response to iron starvation requires t
15 ignal previously not known to be produced by B. cenocepacia as well as pyochelin-type siderophores we
17 cies as zmpA and was detected in B. cepacia, B. cenocepacia, B. stabilis, B. ambifaria, and B. pyrroc
18 ere we compare metabolomes of three clinical B. cenocepacia strains in synthetic CF sputum medium (SC
19 Within the Burkholderia cepacia complex, B. cenocepacia is the most common species associated wit
20 ile they share similar genetic compositions, B. cenocepacia and B. multivorans exhibit important diff
21 ectron acceptors under anaerobic conditions, B. cenocepacia and B. multivorans used fermentation rath
25 ition of mROS or the absence of GSDMD during B. cenocepacia infections reduces autophagy which plays
26 receptor 5 (TLR5) contributes to exacerbate B. cenocepacia-induced lung epithelial inflammatory resp
27 t that P. aeruginosa alginate may facilitate B. cenocepacia infection by interfering with host innate
28 e whether P. aeruginosa alginate facilitates B. cenocepacia infection in mice, cystic fibrosis transm
29 their ability to secrete IL-1beta following B. cenocepacia infection, suggesting that a deficiency i
31 vity of TecA is necessary and sufficient for B. cenocepacia-triggered lung inflammation and also prot
32 pid A explaining the ability of hypoacylated B. cenocepacia LPS to promote proinflammatory responses
33 or transport appears to dramatically impact B. cenocepacia viability, supporting the assignment of o
34 pid A, suggesting lipid A penta-acylation in B. cenocepacia is required not only for bacterial growth
35 nto the control of cable pilus biogenesis in B. cenocepacia and provide evidence for regulation of cb
36 lagellin subunit, fliCII, was constructed in B. cenocepacia K56-2 and tested in a murine agar bead mo
37 dy highlights strain specific differences in B. cenocepacia virulence mechanisms important for unders
41 s a natural substrate for the efflux pump in B. cenocepacia and imply that the environment of low iro
42 our highly conserved periplasmic residues in B. cenocepacia ArnT, tyrosine-43, lysine-69, arginine-25
43 identified functional pathways that indicate B. cenocepacia can produce a wider array of virulence fa
44 an pyrin is required to detect intracellular B. cenocepacia leading to IL-1beta processing and releas
47 growth capabilities of B. cenocepacia J2315, B. cenocepacia K56-2, and B. multivorans ATCC 17616 on 1
50 ole of Cbl pili and the adhesin in mediating B. cenocepacia binding to and transmigration across squa
54 rst comprehensive genome-phenome analyses of B. cenocepacia infection in cystic fibrosis lungs and se
55 edictions to in vitro growth capabilities of B. cenocepacia J2315, B. cenocepacia K56-2, and B. multi
62 ne and M3 significantly increased killing of B. cenocepacia and P. aeruginosa in CF MDMs in a dose-de
64 By immunoscreening an expression library of B. cenocepacia isolate BC7, we identified a large gene (
65 man, J2315 is representative of a lineage of B. cenocepacia rarely isolated from the environment and
66 ells infected with T6SS-defective mutants of B. cenocepacia, suggesting that the inflammatory reactio
67 Further, alginate decreased phagocytosis of B. cenocepacia by professional phagocytes both in vivo a
69 ts show that the intracellular processing of B. cenocepacia is similar in both professional and nonpr
72 e-scale metabolic network reconstructions of B. cenocepacia J2315 and B. multivorans ATCC 17616 in pa
75 ss differential expression, protein spots of B. cenocepacia and B. multivorans that were unique or di
77 is unique in comparison to other strains of B. cenocepacia, highlighting the genomic plasticity of t
81 million bases of cDNA from 2 closely related B. cenocepacia strains (one isolated from a CF patient a
82 tro findings and showed that GSDMD restricts B. cenocepacia infection and dissemination and stimulate
83 ur in vitro study shows that GSDMD restricts B. cenocepacia replication within macrophages independen
84 remain confined to the endobronchial spaces, B. cenocepacia can traverse airway epithelium to cause b
86 ce were differentially regulated, suggesting B. cenocepacia experiences a dramatic shift in metabolis
87 Together, these results demonstrate that B. cenocepacia flagella contribute to virulence in an in
90 which supports the clinical observation that B. cenocepacia is more virulent than B. multivorans.
96 hains and the aminoarabinose residues in the B. cenocepacia lipid A allow exposure of the fifth acyl
100 nome of the previously described therapeutic B. cenocepacia podophage BcepIL02 and its close relative
104 uble-edged functions of GSDMD in response to B. cenocepacia infection and shows the importance of GSD
105 GSDMD promotes inflammation in response to B. cenocepacia through mediating the release of inflamma
107 or ASC induced a robust IL-1beta response to B. cenocepacia, which correlated with enhanced host cell
110 pression of either cblS or cblR in wild-type B. cenocepacia strain BC7 led to a significant increase,
113 activity within and between genomovars, with B. cenocepacia strains possessing the greatest cytokine
114 P-pyrin and ASC (YFP-ASC) were infected with B. cenocepacia and analyzed for inflammasome activation.
115 e regulator knockout mice were infected with B. cenocepacia strain BC7 suspended in either phosphate-
116 riments revealed that patients infected with B. cenocepacia, Burkholderia multivorans, B. pseudomalle
117 hat in human mononuclear cells infected with B. cenocepacia, pyrin associates with caspase-1 and ASC