ライフサイエンスコーパス: B. henselae

1 B. henselae and B. quintana, the organisms that cause ba

2 B. henselae bacteremia was detected in 89% of the 47 nat

3 B. henselae can be difficult to culture axenically, and

4 B. henselae DNA was detected in 34% of 132 fleas, with s

5 B. henselae DNA was identified in 27 of 42 (64%) histolo

6 B. henselae induction of VEGF, IL-1beta, and IL-8 outlin

7 B. henselae target DNA was amplified from 100% of sample

8 B. henselae was identified in 8 of 10 serologically posi

9 B. henselae, B. quintana, and C. burnetii seropositivity

10 B. henselae-mediated inhibition of apoptosis, as indicat

11 A total of 118 B. henselae strains were delineated into 12 sequence typ

12 Out of 22 B. henselae-positive blood samples, isolates were obtain

13 roup 1), 10(8) (group 2), or 10(6) (group 3) B. henselae or with saline (group 4) or were not inocula

14 The 16S rRNA type was determined for 49 B. henselae isolates.

15 o gfp were examined by flow cytometry, and a B. henselae groEL promoter fusion which induced expressi

16 We identified a B. henselae transposon mutant that constitutively expres

17 All B. henselae-inoculated cats were bacteremic by 2 weeks a

18 Bartonella spp. naturally infected dogs (all B. henselae IFA seroreactive) and Group II: 34 Bartonell

19 onii subsp. berkhoffii, B. clarridgeiae, and B. henselae), highly suggestive of Bartonella endocardit

20 Early recognition of HLH and B. henselae through liver biopsy and serological tests l

21 M antibodies against Bartonella quintana and B. henselae.

22 cat had high Bartonella antibody titers and B. henselae type I DNA was detected in the damaged aorti

23 pattern of ocular disease in AIDS-associated B. henselae infections is poorly delineated; unusual man

24 Our study strongly associates B. henselae with cat scratch disease, suggesting that it

25 pecies (i.e., B. vinsonii subsp. berkhoffii, B. henselae, and B. clarridgeiae) that are currently rec

26 microvascular endothelial cells (HMEC-1) by B. henselae resulted in the formation of well-defined va

27 ompare it to infections with those caused by B. henselae and B. clarridgeiae.

28 Infection of human endothelial cells by B. henselae resulting in IL-8 production likely plays a

29 ression appears to be a strategy employed by B. henselae to survive in the arthropod vector and the m

30 tients > 4 weeks into illness) and confirmed B. henselae as the causative species.

31 We designed a study to detect B. henselae in archival biopsies by polymerase chain rea

32 cell is recruited to the endothelium during B. henselae infection and then contributes to bacterial-

33 ontributing to the angiogenic process during B. henselae infection by infiltrating BA lesions and sec

34 nfestation was a significant risk factor for B. henselae bacteremia (odds ratio = 2.82, 95% confidenc

35 California were significant risk factors for B. henselae seropositivity.

36 ith 21.2% of sera from patients positive for B. henselae immunoglobulin G antibodies by indirect immu

37 gooses previously identified as positive for B. henselae.

38 e as a previously unrecognized reservoir for B. henselae.

39 ia, and 138 (51%) cats were seropositive for B. henselae.

40 -like factor autotransporter gene (cfa) from B. henselae.

41 on of an antigenic autotransporter gene from B. henselae.

42 lture was determined with blood samples from B. henselae-infected cats.

43 Blood specimens from B. henselae-infected cats were collected in both EDTA an

44 Furthermore, supernatants from B. henselae-infected HMEC-1 were able to induce chemotax

45 nselae with variations in the 16S rRNA gene, B. henselae type I and type II.

46 Heterogeneous B. henselae-specific IgG reactivity with numerous protei

47 We also investigated the role of IL-8 in B. henselae-induced endothelial cell proliferation and c

48 ransposon mutagenesis to screen for genes in B. henselae heme binding and uptake pathways.

49 Overexpression of hbpC in B. henselae increased resistance to heme toxicity, impli

50 ein (GFP) gene was expressed on a plasmid in B. henselae, and GFP-expressing bacteria were visualized

51 at a homologue of this antigen is present in B. henselae.

52 17-kDa antigen gene, which replaces virB5 in B. henselae, was also demonstrated at the protein level

53 In the immunocompromised individual, B. henselae-induced angiogenesis, or bacillary angiomato

54 most often responsible for human infection, B. henselae and B. quintana, cause prolonged febrile ill

55 Adherence of intact B. henselae to HUVECs was inhibited by increasing concen

56 in (GFP) gene (gfpmut3) and transformed into B. henselae by electroporation.

57 BclI-EcoRI DNA fragment expresses a 120-kDa B. henselae protein immunoreactive with 21.2% of sera fr

58 erns were variable, one approximately 83-kDa B. henselae protein (Bh83) was immunoreactive with all C

59 mice and was reactive with rabbit anti-live B. henselae and mouse anti-Pap31 antibodies by Western b

60 ated human THP-1 macrophages exposed to live B. henselae.

61 The ability to model B. henselae invasion of feline RBC in vitro should permi

62 n and deletion of hbpC affect the ability of B. henselae to infect the cat host.

63 n this study, we investigated the ability of B. henselae to upregulate MCP-1 gene expression and prot

64 , the sensitivity and diagnostic accuracy of B. henselae SA2 WB were higher than those of B. henselae

65 of Escherichia coli or the 43-kDa antigen of B. henselae.

66 om the cat were reactive against antigens of B. henselae (titer, 1,024), B. quintana (titer, 128), an

67 and parenchymal peliosis, and in the case of B. henselae cat-scratch disease.

68 s presented herein denote the circulation of B. henselae STs with zoonotic potential in mongooses wit

69 contribution of antibodies to the control of B. henselae in cats.

70 osplenic cat scratch disease by detection of B. henselae DNA in the orbital abscess fluid.

71 ther improve the sensitivity of detection of B. henselae.

72 lonization that promote the establishment of B. henselae infections in vivo.

73 Promoter fusions of B. henselae chromosomal DNA to gfp were examined by flow

74 Thus, expression of the virB genes of B. henselae is induced in bacteria, which have invaded h

75 n this study we examined the interactions of B. henselae Pap31 with fibronectin (Fn), heparin (Hep),

76 than tubes containing EDTA for isolation of B. henselae and suggest that, for cat blood, collection

77 A cosmid library of B. henselae ATCC 49793 was constructed using SuperCos1 i

78 Frozen blood yielded the largest number of B. henselae colonies for any of the schedules tested.

79 licting reports describe the pathogenesis of B. henselae in the cat.

80 a role for microglia in the pathogenesis of B. henselae-associated neurological disease.

81 were tested individually for the presence of B. henselae DNA by PCR.

82 to determine the longitudinal prevalence of B. henselae bacteremia, the prevalence of B. henselae in

83 of B. henselae bacteremia, the prevalence of B. henselae in the fleas infesting these cats, and wheth

84 toxicity, implicating HbpC in protection of B. henselae from the toxic levels of heme present in the

85 reted cohemolysin autotransporter protein of B. henselae.

86 zoonotic potential in mongooses with risk of B. henselae transmission to humans.

87 passive antibody to the homologous strain of B. henselae did not prevent bacteremia.

88 at B. henselae LSU16 is a virulent strain of B. henselae in cats and propose that the virulence of B.

89 In this study, we characterized a strain of B. henselae termed LSU16.

90 s (0.5%) were coinfected with two strains of B. henselae with variations in the 16S rRNA gene, B. hen

91 pling to explore the population structure of B. henselae in the United Kingdom and to determine the d

92 understand better the long-term survival of B. henselae in cats, we examined the feline humoral immu

93 B. henselae SA2 WB were higher than those of B. henselae SA2 IFA testing.

94 In the wild-type strain, transcription of B. henselae hbpC was upregulated at arthropod temperatur

95 oxacin may prove useful for the treatment of B. henselae infections.

96 ae in cats and propose that the virulence of B. henselae in cats is strain dependent.

97 omatosis, but immunohistochemistry ruled out B. henselae and B. quintana.

98 om naturally infected cats was used to probe B. henselae total membranes to detect commonly recognize

99 , 2, 4, and 2 were positive for B. quintana, B. henselae, and C. burnetii, respectively, by the dPCR

100 e cell lysate fractions from closely related B. henselae, although possessing significant mitogenicit

101 Some B. henselae SA2 immunodominant proteins were recognized

102 etected infection with a Bartonella species (B. henselae or B. vinsonii subsp. berkhoffii) in blood s

103 Two species, B. henselae and B. quintana, have been associated with b

104 e eye by Parinaud's oculoglandular syndrome; B. henselae is the most common cause.

105 lly infected with Bartonella spp. other than B. henselae Additional research is necessary to more ful

106 We conclude that B. henselae LSU16 is a virulent strain of B. henselae in

107 I found that B. henselae induces long-term endothelial survival and t

108 We hypothesize that B. henselae-mediated interaction with immune cells, name

109 , two-dimensional immunoblots indicated that B. henselae LPS and members of the Hbp family of protein

110 This study indicates that B. henselae or B. clarridgeiae can induce chronic infect

111 One hypothesis for this discrepancy is that B. henselae strains vary in their zoonotic potential.

112 Previously, it has been shown that B. henselae infection can result in production of the ch

113 Pap31 is an Fn-binding protein mediating the B. henselae-host interaction(s), and they implicate the

114 Sequencing of the region upstream of the B. henselae virB2 gene revealed a region with sequence h

115 d that HbpC binds hemin and localizes to the B. henselae outer membrane and outer membrane vesicles.

116 elizabethae, 12.5%; to B. quintana, 9.5%; to B. henselae, 3.5%; to Seoul virus, 0.5%; and to Ricketts

117 B. koehlerae was more closely related to B. henselae than to B. clarridgeiae by protein profile,

118 and hepatocytes produce IL-8 in response to B. henselae infection.

119 amined the feline humoral immune response to B. henselae outer membrane (OM) proteins in naturally an

120 cats developed strong antibody responses to B. henselae, as determined by Western blot analysis and

121 moterless vector pANT3 and used to transform B. henselae.

122 onstrate that the cat flea readily transmits B. henselae to cats.

123 ved from bacteremic cattery cats transmitted B. henselae to five SPF kittens in two separate experime

124 rther revealed similar banding patterns when B. henselae was reacted against the Ig isotypes IgG and

125 the fleas infesting these cats, and whether B. henselae is transmitted experimentally to cats via fl

126 is study was undertaken to determine whether B. henselae infects feline fetal brain cells in vitro.

127 ens should elucidate the mechanisms by which B. henselae establishes persistent bacteremic infections

128 Cats bacteremic with B. henselae constitute a large reservoir from which huma

129 Experimental inoculation of cats with B. henselae strains demonstrated that both constitutive

130 hermore, infection of endothelial cells with B. henselae stimulated upregulation of the IL-8 chemokin

131 sampled, 5 cats (1.1%) were coinfected with B. henselae and B. clarridgeiae and 2 cats (0.5%) were c

132 te and OMPs from B. koehlerae, compared with B. henselae and B. clarridgeiae.

133 osis hepatis was associated exclusively with B. henselae.

134 peliosis, 26 (53 percent) were infected with B. henselae and 23 (47 percent) with B. quintana.

135 Cats were readily infected with B. henselae by intravenous inoculation, developed histop

136 ved epitope expression during infection with B. henselae or B. quintana.

137 s epitope conservation during infection with B. henselae or B. quintana.

138 ocyte-enriched cultures were inoculated with B. henselae.

139 Patients with B. henselae infection were identified throughout the stu

140 iters, all eight cats were rechallenged with B. henselae.