ライフサイエンスコーパス: B. parapertussis

1 trisaccharide plus an O-antigen-like repeat (B. parapertussis).

2 d whooping cough in humans (B. pertussis and B. parapertussis).

3 assays for the detection of B. pertussis and B. parapertussis.

4 etella bronchiseptica and the human pathogen B. parapertussis.

5 bronchiseptica (RB50), and other isolates of B. parapertussis.

6 f B. pertussis and B. bronchiseptica but not B. parapertussis.

7 r strains of B. pertussis and two strains of B. parapertussis.

8 lla parapertussis and cloned part of it from B. parapertussis.

9 s and B. holmesii and 68% and 72% identified B. parapertussis.

10 on of two palmitate acyl chains is unique to B. parapertussis.

11 positive samples, 13.99% were identified as B. parapertussis.

12 isolates, which were positive with IS1001 of B. parapertussis.

13 related pathogens, Bordetella pertussis and B. parapertussis.

14 cacy of the acellular vaccine Adacel against B. parapertussis.

15 ot efficiently mediate opsonophagocytosis of B. parapertussis.

16 tection against B. pertussis but not against B. parapertussis.

17 factor is a potential protective antigen for B. parapertussis.

18 e surface and complement-mediated killing of B. parapertussis.

19 larger than that induced by B. pertussis or B. parapertussis.

20 he human-adapted subspecies B. pertussis and B. parapertussis.

21 RB50 (5,338,400 bp; 5,007 predicted genes), B. parapertussis 12822 (4,773,551 bp; 4,404 genes) and B

22 sis and 1,500 CFU/ml or 10 fg/mul of DNA for B. parapertussis A total of 1,103 fresh and residual fro

23 These are the first LPS mutants generated in B. parapertussis and B. bronchiseptica and the first dee

24 Resistance is not efficiently acquired by B. parapertussis and B. bronchiseptica mutants lacking O

25 ers to PT, we examined the ptx genes of both B. parapertussis and B. bronchiseptica to determine whet

26 We have found that B. parapertussis and B. bronchiseptica, unlike B. pertus

27 Our analysis indicates that B. parapertussis and B. pertussis are independent deriva

28 ns of cytokines involved in the clearance of B. parapertussis and immunomodulation that delays effect

29 antigen is a critical protective antigen of B. parapertussis and its inclusion can substantially imp

30 differentiate B. pertussis, B. holmesii, and B. parapertussis and provided protocols and training to

31 mens, 12 were positive (9 B. pertussis and 3 B. parapertussis) and 68 specimens were negative by all

32 Bordetella bronchiseptica, B. pertussis, and B. parapertussis) and its role in their biofilm developm

33 spp., including 4 of B. bronchiseptica, 5 of B. parapertussis, and 5 of B. pertussis, were studied.

34 genes are highly conserved in B. pertussis, B. parapertussis, and B. avium.

35 Bordetella pertussis, B. parapertussis, and B. bronchiseptica are closely rela

36 ecies specificities of Bordetella pertussis, B. parapertussis, and B. bronchiseptica might be explain

37 eptica cluster, which includes B. pertussis, B. parapertussis, and B. bronchiseptica.

38 apid identification of Bordetella pertussis, B. parapertussis, and B. holmesii was developed using mu

39 trisaccharide on the LPS core is present in B. parapertussis, and further suggests that the wild-typ

40 d in B. pertussis and B. holmesii; IS1001 of B. parapertussis; and the IS1001-like sequence of B. hol

41 the toxins encoded by B. bronchiseptica and B. parapertussis are active.

42 Although both B. pertussis and B. parapertussis are more closely related to B. bronchis

43 Here we show that B. pertussis and B. parapertussis are predominantly differentiated from B

44 pertussis Tohama I, B. pertussis 18-323, and B. parapertussis ATCC 15311.

45 Forty-eight hours after infection, wild-type B. parapertussis bacteria but not the O antigen-deficien

46 ng B. bronchiseptica but not B. pertussis or B. parapertussis bacterial numbers during the first 72 h

47 The passive transfer of sera raised against B. parapertussis, but not B. parapertussis Deltawbm, red

48 Interestingly, B. parapertussis, but not B. pertussis, produces an O an

49 B. parapertussis can also cause whooping cough, and B. b

50 ed efficient protection against a subsequent B. parapertussis challenge.

51 Thus, the O antigen of B. parapertussis confers asymmetrical cross-immunity bet

52 n, and found that both B. bronchiseptica and B. parapertussis contain at least certain of these genes

53 B. parapertussis contains a putative pagP homolog (encod

54 es with previously confirmed B. pertussis or B. parapertussis data and with data from 50 contrived B.

55 In B. bronchiseptica and B. parapertussis, delta wlb mutants also lacked O-antige

56 (LPS), which contains the O antigen, but not B. parapertussis Deltawbm LPS drastically improved the e

57 The B. parapertussis Deltawbm mutant was severely defective

58 th an isogenic mutant lacking the O antigen, B. parapertussis Deltawbm, induced antibodies that recog

59 era raised against B. parapertussis, but not B. parapertussis Deltawbm, reduced B. parapertussis load

60 B. pertussis and B. parapertussis Deltawlb mutants were also defective co

61 commercial laboratories for B. pertussis and B. parapertussis detection.

62 Since B. parapertussis did not cause severe disease in IL-1R(-

63 In contrast, LpxA from B. parapertussis did not display relaxed specificity but

64 tingly, serum antibody-mediated clearance of B. parapertussis did not require Fc receptors that are r

65 a Assay, which detects both B. pertussis and B. parapertussis directly from nasopharyngeal swab speci

66 ase in TLR4-deficient mice, B. pertussis and B. parapertussis do not.

67 el of infection, immunization with wild-type B. parapertussis elicited a strong antibody response to

68 tica has a wide host range, B. pertussis and B. parapertussis evolved separately from a B. bronchisep

69 itated the simple and effective isolation of B. parapertussis from ovine nasal swabs and, in successf

70 n of leukocytes in lungs and in clearance of B. parapertussis from the lungs.

71 d as an improved selective medium to isolate B. parapertussis from the nasal cavities of conventional

72 Bordetella pertussis, B. bronchiseptica, and B. parapertussis genome assemblies permitted the identif

73 The increasing incidence of B. parapertussis has been attributed to the lack of cros

74 . pertussis have been well studied, those of B. parapertussis have not.

75 ctious bordetellae, Bordetella pertussis and B. parapertussis, have emerged in historical times as co

76 te according to pathogen host range and that B. parapertussis(hu) most likely acquired its fhaS allel

77 Notably, the genes present in B. parapertussis(hu) strains were pseudogenes, and the g

78 ludes both human-infective (B. pertussis and B. parapertussis(hu)) and non-human-infective (B. bronch

79 e of human-derived Bordetella parapertussis (B. parapertussis(hu)) contains a large in-frame deletion

80 owing inoculation with B. pertussis, but not B. parapertussis, IL-1R(-/-) mice showed elevated bacter

81 (Deltawbm) mutants of B. bronchiseptica and B. parapertussis in a variety of assays relevant to natu

82 Attempts to assess the prevalence of B. parapertussis in conventionally reared sheep by nasal

83 ovement in the detection of B. pertussis and B. parapertussis in nasopharyngeal specimens.

84 tecting and differentiating B. pertussis and B. parapertussis in nasopharyngeal swab specimens.

85 arrant investigation of the relative role of B. parapertussis in the resurgence of whooping cough.

86 adapting to infect humans, B. pertussis and B. parapertussis independently modified their LPS to red

87 Together, these data indicate that B. parapertussis induces the production of IL-10, which

88 ronchiseptica, while no role for TLR4 during B. parapertussis infection has been described.

89 B. pertussis vaccines have little effect on B. parapertussis infection or disease suggest that the p

90 Immunity induced by B. parapertussis infection protected against subsequent

91 In addition, nine cases of B. parapertussis infection were also confirmed by using

92 It was concluded that B. parapertussis infections are more common than previou

93 After finding that several children with B. parapertussis infections exhibited modest antibody ti

94 etection and distinction of B. pertussis and B. parapertussis infections within 2 h.

95 ussis infections but did not protect against B. parapertussis infections.

96 The O antigen of B. parapertussis inhibited binding of antibodies to the

97 Here, we evaluated the outcome of B. parapertussis innate interaction with human macrophag

98 It was previously shown that B. parapertussis is able to avoid bacterial killing by p

99 resent study explores the mechanism by which B. parapertussis is cleared from the lower respiratory t

100 pectrometry analysis revealed that wild-type B. parapertussis lipid A consists of a heterogeneous mix

101 The addition of 10 microg of purified B. parapertussis lipopolysaccharide (LPS), which contain

102 , but not B. parapertussis Deltawbm, reduced B. parapertussis loads in the lower respiratory tracts o

103 0-fold more stimulatory than B. pertussis or B. parapertussis LPS, respectively.

104 ocytes accumulated in the lungs, and cleared B. parapertussis more rapidly.

105 These results highlight the need for B. parapertussis opsonic antibodies to induce bacterial

106 s were pseudogenes, and the genes present in B. parapertussis(ov) strains were expressed at significa

107 d non-human-infective (B. bronchiseptica and B. parapertussis(ov)) strains.

108 s contains a putative pagP homolog (encoding B. parapertussis PagP [PagPBPa]), but its role in the bi

109 B. pertussis positive and 0.2% (n = 2) were B. parapertussis positive.

110 We also observed a notable increase in B. parapertussis positivity on nonpanel PCR tests in the

111 ations in the locus in B. bronchiseptica and B. parapertussis prevent O-antigen biosynthesis and prov

112 The toxin encoded by the B. parapertussis ptx genes appeared more labile in cultu

113 The B. bronchiseptica and B. parapertussis recipients were now able to biosynthesi

114 lymerase chain reaction results positive for B. parapertussis reported during October 2011-May 2012 w

115 quences IS481 and IS1001 of B. pertussis and B. parapertussis, respectively, and is performed using t

116 umulation of CD4(+) T cells in the lungs and B. parapertussis-responsive IFN-gamma-producing cells in

117 us wlb locus of Bordetella bronchiseptica or B. parapertussis restored partial sensitivity to Ba1.

118 tussis does not express the O antigen, while B. parapertussis retains it as a dominant surface antige

119 ve strains of B. pertussis and one strain of B. parapertussis revealed extensive divergence of gene o

120 rtussis data and with data from 50 contrived B. parapertussis samples, the proportions of positive an

121 rtussis strain 18323 and an ovine isolate of B. parapertussis show significant transcription of the g

122 ical bordetellae, including B. pertussis and B. parapertussis, something the current vaccines do not

123 In vitro B. parapertussis-stimulated macrophages produced IL-10,

124 The assay detected 16/18 unique B. pertussis/B. parapertussis strains.

125 ed efficacy of B. pertussis vaccines against B. parapertussis suggest a lack of cross-protective immu

126 eir control and clearance of B. pertussis or B. parapertussis, suggesting that IgA is not crucial to

127 t that in the absence of opsonic antibodies, B. parapertussis survives inside macrophages by preventi

128 that might be misclassified as pertussis if B. parapertussis testing is not performed.

129 culture, PCR (both nonpanel B. pertussis and B. parapertussis tests and those included as part of a r

130 large genetic locus in B. bronchiseptica and B. parapertussis that is required for O-antigen biosynth

131 for the mechanism of protective immunity to B. parapertussis that is similar but distinct from that

132 9.0% for B. pertussis and 100% and 99.7% for B. parapertussis The Aries Bordetella Assay provides acc

133 (-1) for B. pertussis and 213 CFU.ml(-1) for B. parapertussis The assay detected 16/18 unique B. pert

134 There were no samples positive for B. parapertussis The PPA and NPA of the Aries BA were 61

135 o the bacterial surface and was required for B. parapertussis to colonize mice convalescent from B. p

136 that O antigen contributes to the ability of B. parapertussis to colonize the respiratory tract durin

137 These data indicate that O antigen enables B. parapertussis to efficiently colonize the lower respi

138 in the absence of opsonins, O antigen allows B. parapertussis to inhibit phagolysosomal fusion and to

139 The O antigen targets B. parapertussis to lipid rafts that are retained in the

140 In addition, O antigen was required for B. parapertussis to systemically spread in complement-su

141 In other assays, B. parapertussis was distinct from all other species (in

142 B. parapertussis was more similar to B. bronchiseptica t

143 erestingly, an O antigen-deficient strain of B. parapertussis was not defective in colonizing mice la

144 B. parapertussis was not detected in any specimens.

145 swabs from conventionally reared sheep, and B. parapertussis was recovered from 31.5% of the samples

146 The overall PPA and NPA for B. parapertussis were 96.7% and 100%, respectively.

147 In the case of B. parapertussis, which normally does not synthesize an

148 The persistence of Bordetella pertussis and B. parapertussis within vaccinated populations and the r

149 from a range of bacteria, is altered in the B. parapertussis WlbH protein.