ライフサイエンスコーパス: BMP-7

1 abolic factor, bone morphogenetic protein-7 (BMP-7).

2 on pSMAD1/5/9 were observed when induced by BMP-7.

3 1 week later by an intraventricular dose of BMP-7.

4 f two other known GC mitogens, activin-A and BMP-7.

5 ived both the enalapril and the high dose of BMP-7.

6 I TGF-beta family receptor for activins and BMP-7.

7 ct with tissues expressing BMP-2, BMP-4, and BMP-7.

8 endogenous epidermis inducers are BMP-4 and BMP-7.

9 BMP genes leading to expression of BMP-4 and BMP-7.

10 F-1 suppresses noggin, a potent inhibitor of BMP-7.

11 CM with BMP-7, or CM with both TGFbeta1 and BMP-7.

12 iabetic kidney disease by AGE inhibitors and BMP-7.

13 (12.5 mg/kg body wt per d), group 3 received BMP-7 (50 or 300 microg/kg), and group 4 received both t

14 Interestingly, we found that miR-22 and BMP-7/6 are in a regulatory feedback circuit, whereby no

15 mice demonstrated a significant increase in BMP-7/6 expression and their downstream targets.

16 y analyzing the potential miRNAs that target BMP-7/6 in silica, we identified miR-22 as a potent miRN

17 we examined the regulatory role of miRNAs on BMP-7/6 signaling.

18 We found that expression levels of BMP-7/6 were significantly elevated in the kidneys of th

19 ck circuit, whereby not only miR-22 inhibits BMP-7/6, but miR-22 by itself is induced by BMP-7/6.

20 dentified miR-22 as a potent miRNA targeting BMP-7/6.

21 BMP-7/6, but miR-22 by itself is induced by BMP-7/6.

22 Bone morphogenetic protein 7 (BMP-7), a member of the TGFbeta superfamily, is also inv

23 BMP-7, a member of the transforming growth factor-beta (

24 by the Smad-binding sites was responsive to BMP-7, a protein with known protective effects against i

25 tion treatment, IGF-1 activated Smad2, while BMP-7 activated Smad1/5/8 and Smad3, thus inducing all S

26 Furthermore, BMP-7 activated the VEGF promoter.

27 Here, we describe a new MMP-dependent BMP-7 activation mechanism from ECM-targeted pools via s

28 binary complexes such as BMP-2/BMPR Ia-ECD, BMP-7/ActRII-ECD, and activin/ActRIIb-ECD, respectively,

29 uorescent protein (Ad-GFP or control virus), BMP-7 (Ad-BMP-7), or an antagonist of BMP bioactivity, n

30 h affinity ActRIIB ligand, whereas BMP-2 and BMP-7 affinities for ActRIIB are at least 100-fold lower

31 at the corresponding positions of BMP-2 and BMP-7 allowed for molecular engineering of recombinant B

32 Maintenance of BMP-7 also reduces glomerular fibrosis and interstitial

33 BMP-7 also reverses vascular calcification in CKD, and r

34 the effects of bone morphogenetic protein-7 (BMP-7), also named osteogenic protein-1 (OP-1), on the p

35 phorylation or nuclear accumulation, nor did BMP-7 alter phosphorylated Smad3 dephosphorylation or de

36 ameliorated by bone morphogenetic protein-7 (BMP-7), an efficacious agent in treating animal models o

37 oblastoma x glioma hybrid cells treated with BMP-7, an inducer of L1 and neural cell adhesion molecul

38 -mesenchymal transition and survival factors BMP-7 and Bcl-2 and preserved capillary density in rat c

39 R and functional activity assays with BMP-2, BMP-7 and GDF-5 as ligands.

40 ssay, complex formation was demonstrated for BMP-7 and growth and differentiation factor-8 (GDF-8), w

41 Combination growth factor therapy using BMP-7 and IGF-1 may have considerable promise in the tre

42 ucleus pulposus (NP) cells were treated with BMP-7 and IGF-1.

43 Pax-2, Sox-3, BMP-7 and Notch are all expressed in placodal ectoderm f

44 Oligodendrocytes expressed BMP-2 and BMP-7 and pSMAD1/5/8.

45 or inhibitor-1 promoter in PTCs treated with BMP-7 and TGF-beta compared with TGF-beta alone.

46 sults provide evidence of cross talk between BMP-7 and TGF-beta1 in the regulation of EMT in health a

47 ysomes during oocyte maturation, whereas the BMP-7 and XSTK9 mRNAs were recruited during the early st

48 utic efficacy of bone morphogenic protein-7 (BMP-7) and inhibitors of advanced glycation end products

49 Bone morphogenetic protein-7 (BMP-7) and its related BMP-6 have recently emerged as ke

50 c potential of bone morphogenetic protein 7 (BMP-7) and transforming growth factor beta1 (TGFbeta1)-i

51 signaling by the low affinity ligands BMP-2, BMP-7, and BMP-9.

52 f the BMP subfamily, including BMP-2, BMP-4, BMP-7, and GDF-5, with similar kinetics and ligand bindi

53 , noggin, attenuated the anabolic effects of BMP-7, and noggin was substantially increased by BMP-7,

54 e also measured the effects of BMP-2, BMP-4, BMP-7, and the BMP inhibitor LDN-193189 on the expressio

55 of IGFBP-3 with bone morphogenic protein-7 (BMP-7), another member of the TGF-beta superfamily, resu

56 s absent in the liver, but the receptors for BMP-7 are present on adult hepatocytes.

57 ibrillin-1 and bone morphogenetic protein-7 (BMP-7) are mediated by the prodomain of growth factor co

58 lectively, our results argue for the role of BMP-7 as a kidney- and bone-produced endogenous regulato

59 o establish a role for endogenous glomerular BMP-7 as an autocrine regulator of podocyte integrity in

60 successfully reversed with a bone anabolic, BMP-7, associated with a reduction in plasma phosphorus.

61 The BMP-7-binding affinity of site-specific variants of Nogg

62 BMPR1A, another BMP type 1 receptor, whereas BMP-7 binds to ACVR1 to activate SMAD1/5/9 signaling.

63 onstrated that bone morphogenetic protein-7 (BMP-7) blunted the development of fibrosis in a rat mode

64 d the expression of BMP-2, BMP-4, BMP-5, and BMP-7; BMP type II receptor (BMPRII); and phosphorylated

65 d as being in contact with the ligand in the BMP-7-BMPR-II complex but are found mutated in genetic d

66 The expression of BMP-2, BMP-4, BMP-5, BMP-7, BMPRII, and pSMAD1/5/8 was quantified by immunost

67 d neurons expressed BMP-2, BMP-4, BMP-5, and BMP-7; BMPRII; and pSMAD1/5/8.

68 ic growth was induced by exposure to NGF and BMP-7 (bone morphogenetic protein-7).

69 ect on the affinity of chordin for BMP-4 and BMP-7 but C-terminal cleavage increases the efficacy of

70 cal role as a negative feedback regulator of BMP-7 but not BMP-6-induced biological responses.

71 GF-beta family ligands Activin B, BMP-6, and BMP-7, but not the frog Cerberus ligand BMP-2.

72 Epithelial expression is also seen for Bmp-7, but transcripts are less dramatically upregulated

73 t maintenance of renal (especially podocyte) BMP-7 by transgenic expression reduces diabetic renal in

74 e morphogenetic proteins (BMP)-2, BMP-4, and BMP-7 can promote the development of tyrosine hydroxylas

75 actions with other signaling pathways, chick BMP-7 (cBMP-7) was cloned.

76 thods elucidated the interaction between the BMP-7 complex and the extracellular domains of its type

77 hadowing electron microscopy of molecules of BMP-7 complex bound to fibrillin-1 confirmed these findi

78 pabilities between the growth factor and the BMP-7 complex in multiple in vitro bioactivity assays.

79 However, upon binding to fibrillin-1, the BMP-7 complex is rendered into a closed ring shape, whic

80 two prodomain propeptide chains and that the BMP-7 complex is structurally similar to the small trans

81 This interaction may target the BMP-7 complex to fibrillin microfibrils in the extracell

82 t upon prodomain binding to fibrillin-1, the BMP-7 complex undergoes a conformational change, which d

83 The BMP-7 prodomain and BMP-7 complex, but not the separated growth factor dimer

84 evidence that PD cleavage by MMP-13 leads to BMP-7 CPLX disintegration and bioactive GF release.

85 demonstrated that PD-dependent targeting of BMP-7 CPLXs to the extracellular fibrillin microfibril (

86 of oral phosphate binders or intraperitoneal BMP-7 decreased expression of osterix and aortic mineral

87 BMP-7 did not inhibit Smad3 phosphorylation or nuclear a

88 It is concluded that maintenance of renal BMP-7 during the evolution of diabetic nephropathy reduc

89 Compared to vehicle-rats, BMP-7 enhanced glucose utilization in the basal ganglia

90 cific expression profile of genes, including Bmp-7, EphA5/Cek-7, EphA4/Cek-8, and several chick homol

91 BMP-7 expression is absent in the liver, but the recepto

92 BMP-7 expression is highest in the kidney, and its genet

93 Bmp-7 expression patterns demonstrate dissociation in th

94 ncreased bone sialoprotein, osteocalcin, and BMP-7 expression.

95 a loss of renal bone morphogenic protein-7 (BMP-7) expression.

96 The osseous lesions treated by Ad-BMP-7 gene delivery demonstrated rapid chrondrogenesis,

97 This study utilized ex vivo BMP-7 gene transfer to stimulate tissue engineering of a

98 ulation of bovine NP cells by both IGF-1 and BMP-7 greatly potentiated anabolism through complementar

99 l group and the CKD low-phosphate/calcitriol/BMP-7 groups had reduced phosphate levels compared with

100 We found that BMP-2, BMP-4, BMP-6, and BMP-7 had no direct effect on prostate cancer cell growt

101 Furthermore, BMP-7 had no effect on Smad3 signaling after siRNA-media

102 Bone morphogenetic protein-7 (BMP-7) has been shown to enhance dendritic growth and im

103 However, BMP-7 immunolocalization in other tissues known to be ac

104 Bone morphogenetic protein-7 (BMP-7) improves outcome in animal models of fibrotic ren

105 arent, suggesting that immunolocalization of BMP-7 in certain tissues represents specific extracellul

106 Transgenic expression of BMP-7 in glomerular podocytes and proximal tubules preve

107 ptor utilization by BMP-2, BMP-4, BMP-6, and BMP-7 in primary human mesenchymal stem cells (hMSC), a

108 pendent reversal of TGF-beta1-induced EMT by BMP-7 in renal tubular epithelial cells and mammary duct

109 few studies have addressed the expression of BMP-7 in reproductive tissues, and the role of BMP-7 rem

110 ed nuclear accumulation of Smad-1 induced by BMP-7 in sympathetic neurons, suggesting a direct enhanc

111 his report demonstrates that the function of BMP-7 in the oviduct involves the induction of apoptosis

112 Immunolocalization of BMP-7 in tissues like the kidney capsule and skin reveal

113 er exposure to bone morphogenetic protein-7 (BMP-7) in vitro.

114 s ameliorated by the skeletal anabolic agent BMP-7, in part through deposition of phosphate and incre

115 PACAP and VIP reduced BMP-7-induced dendritic growth by approximately 70-90%,

116 In vitro lineage tracing confirmed that BMP-7-induced insulin-expressing cells arise mainly from

117 cyte apoptosis by modulation of TGF-beta and BMP-7-induced pro- and antiapoptotic signals.

118 Furthermore, addition of purified BMP-7 induces apoptosis in primary oviduct cells.

119 Here we report that BMP-7 induces formation of epithelial cell aggregates in

120 Exposure to BMP-7 induces nuclear translocation of Smad1 (Sma- and M

121 BMP-4, BMP-2, and BMP-7 inhibited basal and tumor necrosis factor alpha-st

122 In this setting, BMP-7 inhibits epithelial-mesenchymal transition involvi

123 re, through a cell-autonomous loop BMP-4 and BMP-7 intensified Smad1/5 signaling though a feedforward

124 Furthermore, neither BMP-4 nor BMP-7 interfere with neural induction when misexpressed

125 These results suggest that BMP-7 is able to act as a dopaminotrophic agent without

126 BMP-7 is an important regulator of skeletal remodeling a

127 e physiological circulating concentration of BMP-7 is between 100 and 300 pg/ml.

128 In postnatal life, BMP-7 is expressed primarily in the kidney, and expressi

129 -7 significantly inhibit glomerular lesions, BMP-7 is most effective in the inhibition of tubular inf

130 BMP-7 is secreted as a stable complex consisting of a gr

131 nd biophysical methods are used to show that BMP-7 is secreted as a stable complex consisting of the

132 Bone morphogenic protein-7 (BMP-7) is a key protein involved in liver organogenesis

133 systemic administration of recombinant human BMP-7 leads to repair of severely damaged renal tubular

134 A second BMP, BMP-7, less studied than BMP-2 may have opposing actions

135 he ability of THR-123, a cyclic peptide with BMP-7-like activity, to regenerate beta-cell mass in dia

136 ovel pathway through which administration of BMP-7 may attenuate renal damage.

137 In addition, we provide evidence that BMP-7-mediated repair of renal injury is associated with

138 lular hyaluronan-cell interactions inhibited BMP-7-mediated Smad1 phosphorylation, nuclear translocat

139 Therefore, we hypothesized that BMP-7 might function as an endogenous regulator of adult

140 we hypothesized that systemic treatment with BMP-7 might induce MET involving adult renal fibroblasts

141 tanol also antagonized ethanol inhibition of BMP-7 morphogenesis in NG108-15 cells.

142 We further focused on the BMP-7 mRNA and demonstrated that sequence elements withi

143 Interestingly, the BMP-7 mRNA lacks the previously defined eCPE sequences p

144 3'UTR were necessary for recruitment of the BMP-7 mRNA to polysomes and sufficient to direct the add

145 ies (BMP-7, n=7; vehicle, n=8); LCBF series (BMP-7, n=6; vehicle, n=8).

146 animals groups were studied: LCMRglu series (BMP-7, n=7; vehicle, n=8); LCBF series (BMP-7, n=6; vehi

147 decreased motility, mimicking the effect of BMP-7 on embryonic metanephric mesenchyme to generate ep

148 In this study, we examined the effect of BMP-7 on functional recovery, local cerebral blood flow

149 Here, we studied the effect of BMP-7 on response to TGF-beta in the proximal tubular ce

150 or MG132 reproduced the inhibitory action of BMP-7 on Smad3 signaling.

151 1 (IGF-1) and bone morphogenetic protein 7 (BMP-7) on bovine spine discs and by elucidating the rele

152 BMP-7 or vehicle (volume, 25 microl) was administered in

153 protein (Ad-GFP or control virus), BMP-7 (Ad-BMP-7), or an antagonist of BMP bioactivity, noggin (Ad-

154 genic medium (CM), CM with TGFbeta1, CM with BMP-7, or CM with both TGFbeta1 and BMP-7.

155 ssion of bone morphogenetic protein (BMP)-2, BMP-7, osteopontin, and bone sialoprotein were evaluated

156 vivo when compared with its closely related BMP-7 paralog.

157 nd conserved MMP-13 cleavage site within the BMP-7 PD.

158 o EndMT, whereas bone morphogenic protein 7 (BMP-7) preserved the endothelial phenotype.

159 lost in PTCs after TGF-beta stimulation, but BMP-7 prevented this.

160 We conclude that BMP-7 prevents TGF-beta-mediated loss of the transcripti

161 tivity of chimeric precursors containing the BMP-7 prodomain and BMP-4 mature domain, or vice versa,

162 The BMP-7 prodomain and BMP-7 complex, but not the separated

163 BMP-7 prodomain variants were used to map the critical e

164 We present the nanoscale structure of the BMP-7 prodomain-growth factor complex using electron mic

165 Bone morphogenetic protein 7 (BMP-7) regulates cellular metabolism in embryonic and ad

166 P-7 in reproductive tissues, and the role of BMP-7 remains unclear.

167 BMP-7 restores skeletal anabolic balance in animal model

168 hat neutralization of circulating endogenous BMP-7 results in significantly impaired regeneration of

169 Here we report that BMP-7 reverses TGF-beta1-induced epithelial-to-mesenchym

170 rapeutic administration of recombinant human BMP-7 (rhBMP-7) significantly enhances liver regeneratio

171 systemic administration of recombinant human BMP-7 (rhBMP-7) significantly inhibited EndMT and the pr

172 mutant/KRAS-dependent cells, KRAS stimulates BMP-7 secretion and BMP signaling, leading to TAK1 activ

173 morphogenetic protein (BMP) such as BMP-2 or BMP-7 sets in motion a cascade of cellular events result

174 ol 3-kinase/Akt pathway and thus potentiated BMP-7 signaling in bovine NP cells.

175 n other tissues known to be active sites for BMP-7 signaling is not apparent, suggesting that immunol

176 teraction between Fbn2-rich microfibrils and BMP-7 signaling.

177 ACVR1 is the major receptor for transducing BMP-7 signals in osteoclasts.

178 BMP-7 significantly improved the total neurological scor

179 Interestingly, BMP-7 significantly increased TH-ir in the SN of the non

180 ous locomotor activity was observed, however BMP-7 significantly increased the density of tyrosine hy

181 t although aminoguanidine, pyridoxamine, and BMP-7 significantly inhibit glomerular lesions, BMP-7 is

182 ent caused further downregulation of Klotho, BMP-7, Smad7, and E-cadherin, all of which are associate

183 BMP-7 specifically limited Smad3 but not Smad2 signaling

184 r exhibited Smad1 nuclear translocation upon BMP-7 stimulation.

185 the effect of a potent osteoinductive agent (BMP-7), suggesting a synergism between the soluble facto

186 7, and noggin was substantially increased by BMP-7, suggesting a negative feedback regulatory mechani

187 1/5/9 levels when induced either by BMP-2 or BMP-7, suggesting that ACVR1 is the major receptor for t

188 al skeletal histomorphometry was reversed by BMP-7 therapy to normal values and significantly improve

189 be re-engaged by systemic administration of BMP-7 to mediate repair of tubular injury in a fibrotic

190 search of a mechanism behind the ability of BMP-7 to repair damaged renal tubules, we hypothesized t

191 We therefore tested the potential of BMP-7 to reverse TGF-beta1-induced de novo EMT in a mous

192 The ossicles formed by the BMP-7-transduced HOKC were smaller and more dense than t

193 smaller and more dense than those formed by BMP-7-transduced human gingival fibroblasts (HGF).

194 ty was limited to fibroblasts, we found that BMP-7-transduced human oral keratinocyte cells (HOKC) al

195 hoenolpyruvate carboxykinase promoter-driven BMP-7 transgene and nondiabetic, transgenic mice as well

196 These effects of the BMP-7 transgene occur without changing renal TGF-beta le

197 of messenger RNA levels, was highest in the BMP-7-treated group.

198 is as compared to the control-treated and Ad-BMP-7-treated specimens.

199 29.0+/-4.9 O.D. control vs. 64.4+/-6.9 O.D. BMP-7-treated, p<0.001).

200 lso noted (113.2 ng/g control vs. 198.2 ng/g BMP-7-treated, p<0.01).

201 : 14.8+/-1.19 O.D. control vs. 36+/-2.6 O.D. BMP-7-treated, p<0.05; pars compacta: 29.0+/-4.9 O.D. co

202 arbitrary units) control vs. 50.2+/-4.3 O.D. BMP-7-treated; p<0.05].

203 us BMP-7 treatment, and CM plus TGFbeta1 and BMP-7 treatment (compared with CM treatment alone).

204 BMP-7 treatment corrected the hyperphosphatemia, correct

205 No significant effect of BMP-7 treatment on spontaneous locomotor activity was ob

206 However, BMP-7 treatment reduced Smad3 DNA binding to a consensus

207 ved with CM plus TGFbeta1 treatment, CM plus BMP-7 treatment, and CM plus TGFbeta1 and BMP-7 treatmen

208 at limiting fibrosis of the kidney when the BMP-7 was administered during the progression of fibroti

209 and a treatment protocol used to examine if BMP-7 was beneficial at limiting fibrosis of the kidney

210 ricularly administered neurotrophic factors, BMP-7 was not associated with an increase in the sensiti

211 mologous to the fibrillins, the prodomain of BMP-7 was tested for binding to fibrillin-1 or to LTBP-1

212 ellet was in the group in which TGFbeta1 and BMP-7 were added to the medium.

213 Here, we observed that BMP-7, which preferentially binds to ACVR1, promotes ost

214 structure of the antagonist Noggin bound to BMP-7, which shows that Noggin inhibits BMP signalling b

215 ansforming growth factor-beta (TGF-beta) and BMP-7 with high affinity, suggesting that it may be an i