ライフサイエンスコーパス: BRET

1 BRET data confirmed the role of Lys-14 and Lys-15 in arr

2 BRET data suggested substantial inhibition of CYP1A2-med

3 BRET levels were not altered by pretreatment with seroto

4 BRET of live HEK-293 cells transfected with the subtypes

5 BRET shifts, indicating conformational change, were dete

6 BRET should be particularly useful for testing protein i

7 BRET signals comparable to those obtained from cells coe

8 BRET signals observed between AGS4-RLuc and Galpha(i1)-Y

9 BRET signals were higher for the catalytically inactive

10 BRET studies showed that, like the secretin receptor, bo

11 BRET was applied in conjunction with site-directed mutag

12 BRET was measured as an indication of receptor oligomeri

13 BRET was the lead technique for this identification proc

14 BRET(2) provides a better matched Forster distance to th

15 BRET(50) (BRET(50) represents the relative affinity as a

16 BRET-based binding at the NLuc-hH(3,4)Rs/mH(4)R [pK(d) 8

17 bioluminescence resonance energy transfer 2 (BRET(2)) in STHdh(Q7/Q7) cells.

18 ioluminescence resonance energy transfer(2) (BRET(2)) system showed a 30% increase in the BRET ratio

19 A total of 22 BRET assays have been established for nine RTKs derived

20 vior in an improved competition-based type-3 BRET assay designed to circumvent such artifacts.

21 Using type-4 BRET assays, we investigate heterodimerization among kno

22 energy transfer (BRET) assay, called type-4 BRET, which detects both homo- and heteromeric interacti

23 BRET(50) (BRET(50) represents the relative affinity as acceptor/do

24 A BRET (bioluminescence resonance energy transfer) assay r

25 A BRET assay suggested that DOR phosphorylation promotes r

26 A BRET increase was observed exclusively for the gastric i

27 A BRET-based competition binding assay with 4 was also est

28 te studies of cAMP regulation we developed a BRET (bioluminescence resonance energy transfer) sensor

29 Here we present a BRET approach to monitor ligand binding to G protein-cou

30 Additionally, BRET-based biosensors revealed AC5 can compete with othe

31 uc and alpha2AAR-Galphai2YFP(C352I):AGS4Rluc BRET was not altered by PT treatment or Gbetagamma antag

32 All BRET signals were blocked by the selective D2R antagonis

33 ssays, ex vivo rat aortic ring bioassays and BRET-based biosensor experiments.

34 g, in silico computer-aided drug design, and BRET functional assays, we identified new structural sca

35 FRET and BRET approaches are well established for detecting ligan

36 raTM measurements in bacterial membranes and BRET measurements made on corresponding RAGE constructs

37 orescent proteins, TagRFP and TurboFP635, as BRET acceptors.

38 QDs) are particularly well suited for use as BRET acceptors due to their high quantum yields, large S

39 fluorescent proteins are frequently used as BRET acceptors, both small molecule dyes and nanoparticl

40 e (RLuc) variants RLuc8 and RLuc8.6, used as BRET donors, combined with two red fluorescent proteins,

41 We found basal BRET interactions for some of the receptor combinations

42 m of this interaction using bioluminescence (BRET) and fluorescence (FRET) resonance energy transfer

43 mbled CB1-Galphai complexes were detected by BRET(2) Arachidonyl-2'-chloroethylamide (ACEA), a select

44 Analysis of the binding kinetics by BRET revealed remarkably long intracellular residence ti

45 ned the ability to dimerize, as monitored by BRET, whereas other mutations inhibited both nuclear exc

46 ween CheZ and CheY-P, the quantity sensed by BRET.

47 ressed with wild-type receptor, are shown by BRET(2) to heterodimerize, accounting for their dominant

48 e PRKAR1A from the catalytic PKA subunits by BRET assay.

49 refore developed a full-spectral three-color BRET assay for analyzing the specific activation of each

50 ber of beta-adrenergic agonists by comparing BRET assays of receptor-transducer interactions with Gs,

51 In contrast, BRET between tightly-associating control proteins does n

52 Conventional BRET analyses are generally done at maximal expression l

53 omers on the basis of previous, conventional BRET experiments.

54 eins versus beta-arrestin recruitment in D2R-BRET functional assays.

55 produced no ligand-independent or -dependent BRET.

56 ciferase to open LOV via proximity-dependent BRET.

57 med Arabidopsis or tobacco, we then detected BRET between three pairs of candidate interaction partne

58 DHHC3 BRET in cell membranes was decreased by the addition of

59 f a fluorescent probe that acts as a dynamic BRET biosensor of the intracellular SAM/MTA pool that ov

60 An efficient BRET from Nanoluc-Halotag chimera protein (H-Luc) to a c

61 EGFR BRET assays are highly sensitive to known EGFR ligands [

62 We applied EGFR BRET assays to study the characteristics of somatic EGFR

63 Our results demonstrate that EGFR BRET assays precisely measure the pharmacology and signa

64 Furthermore, we demonstrate that the EGFR BRET assays are a useful tool to study the pharmacology

65 Epidermal growth factor receptor (EGFR) BRET structure-function studies identify the tyrosine re

66 cant resonance transfer signals using either BRET or a morphological FRET assay, further supporting t

67 ptors reduce but do not completely eliminate BRET transfer between receptors.

68 These red light-emitting BRET systems have great potential for investigating PPIs

69 Here, using an endosomal BRET-based assay in a high-throughput screen with the pr

70 light intensity in comparison with existing BRET fusion proteins.

71 First, BRET demonstrated a much reduced efficacy for salmeterol

72 In view of fluorescent probes for BRET-based binding studies and for localizing the H(4)R

73 his review examines the potential of QDs for BRET-based bioassays and imaging, and highlights example

74 As expected, both HO-1 and CYP1A2 formed BRET-detectable complexes with POR.

75 Currently, FRET/BRET assays rely on co-expression of GPCR and G protein,

76 FRET signal comparable to co-expressed FRET/BRET sensors.

77 Functional BRET assays included activation of G proteins with all G

78 conformational fluorescein arsenical hairpin-BRET sensors coupled with high-resolution fluorescence m

79 Thus, ER homodimer and heterodimer BRET assays are applicable to drug screening for dimer-s

80 4 enabled the establishment of a homogeneous BRET-based binding assay suitable for both detailed kine

81 uses enzyme-catalyzed luminescence; however, BRET signals usually have been too dim to image effectiv

82 fic interaction as indicated by a hyperbolic BRET signal in response to increasing PAR4-GFP expressio

83 The inhibitory regulation of GPR-Galpha(i1) BRET by Ric-8A was blocked by pertussis toxin.

84 The enhancement of GPR-Galpha(i1) BRET observed with Ric-8A was further augmented by pertu

85 In BRET experiments, the PAR4 homodimers showed a specific

86 er domains, resulting in a large decrease in BRET efficiency.

87 teractions is quantified through decrease in BRET.

88 Such key improvement in BRET measurement paves the way for the simultaneous moni

89 st, caused a rapid and transient increase in BRET efficiency (BRETEff) between Galphai-Rluc and CB1-g

90 G protein receptor kinase 3, an increase in BRET signal correlated with OR activation mediated by a

91 astrin-4) resulted in the rapid reduction in BRET signal in contrast to the enhancement of such a sig

92 Agonist-induced reduction in BRET signal was also observed for pairs of CCK receptors

93 ation, and this agonist-induced reduction in BRET was blocked by pertussis toxin.

94 PAR4 did not interact with rhodopsin in BRET assays.

95 Deletion of S2 produced ligand-independent BRET for only those pairings normally occurring in the p

96 Ligand-induced BRET changes assessing Gbetagamma-Kir3.1 subunit interac

97 at the standard frameworks used to interpret BRET titration experiments rely on simplifying assumptio

98 Here, using an intramolecular BRET (bioluminescence resonance energy transfer)-based b

99 ophysical experiments with an intramolecular BRET beta-arrestin2 biosensor revealed that osmotic stre

100 data exemplifying the use of intramolecular BRET probes to study other transient receptor potential

101 Even more intriguing, BRET experiments indicate that CTAP (D-Phe-Cys-Tyr-D-Trp

102 e pharmacological characterization involving BRET biosensors, binding studies, electrophysiology, and

103 mputational model analyzing the longitudinal BRET imaging data of antibody-target binding and explori

104 A lower BRET(50) for the alpha(2A)-alpha(2C) heterodimer (0.79 +

105 r/donor ratio required to reach half-maximal BRET [bioluminescence resonance energy transfer] values)

106 The threshold maximum BRET signal was disrupted in a concentration-dependent m

107 R-Kir3 interactions unmodified but modulated BRET between DOR-GalphaoA, DOR-Gbetagamma, GalphaoA-Gbet

108 h the K2A mutations had little effect on net BRET(max) values for the M2 muscarinic acetylcholine (M2

109 Future development of new BRET acceptors should further expand the multiplexing ca

110 The imaging utility of the new BRET vector is shown by constructing a sensor using two

111 This new BRET fusion protein (BRET3) exhibits severalfold improve

112 This new BRET strategy provides a unique platform to assay protei

113 This new BRET vector should facilitate high-throughput sensitive

114 This new BRET vector shows an overall 5.5-fold improvement in the

115 s, as well as a high specific to nonspecific BRET binding signal.

116 efficiency, we report generation of a novel BRET vector by fusing a GFP(2) acceptor protein with a n

117 ing did not significantly alter the observed BRET(2) signal, suggesting that CXCR4 exists as a consti

118 The advantages of BRET include expressing full-length proteins in their na

119 r results demonstrate a novel application of BRET for assessing target engagement within the complex

120 Herein, we report the application of BRET to performing a biorthogonal reaction in living cel

121 The CCD imaging approach of BRET signal is particularly appealing due to its capacit

122 urther expand the multiplexing capability of BRET and improve its applicability and sensitivity for i

123 The combination of BRET/bimolecular luminescence complementation assay reve

124 The dependence of BRET efficiency on acceptor/donor ratio at fixed surface

125 multaneous visualization and quantitation of BRET signal from live cells and cells implanted in livin

126 ng advantage of the critical relationship of BRET efficiency and donor quantum efficiency, we report

127 Here, we present a set of BRET sensors monitoring the activation of the 12 G prote

128 associated with the nonrigorous treatment of BRET data are illustrated for the case of G protein-coup

129 The use of BRET simultaneously simplifies the hardware required for

130 multimerization as well as a new variant of BRET assay that is useful for measuring the interactions

131 Here, we developed an optical BRET-based biosensor, Galpha(i) bONE-GO, that detects en

132 Our BRET study also confirmed that: (1) capsaicin and heat p

133 lly-induced recruitment of local third-party BRET donors or acceptors reliably separates nonspecific

134 VPAC2, and secretin receptors, and performed BRET and morphologic fluorescence resonance energy trans

135 tion was induced, as indicated by a positive BRET signal, on exposure of the cells to bivalent ligand

136 assay time reduction, compared with previous BRET-based work or other technologies.

137 tion-mediated desensitization, also produced BRET signals above background.

138 ated internally by a bioluminescent protein, BRET does not rely on an external light source.

139 s and imaging, and highlights examples of QD-BRET for biosensing and imaging applications.

140 ioluminescence resonance energy transfer (QD-BRET) to detect the protease activity in complex biologi

141 However, quantitative BRET(2) analyses in intact cells indicate a lack of effe

142 inated the disruptive effect on CCK receptor BRET, whereas the other mutant peptide behaved like wild

143 that receptor had no effect on CCK receptor BRET.

144 ly TM VI and VII peptides disrupted receptor BRET.

145 ormation within complexes, reducing receptor BRET signals.

146 drenergic receptor (beta2AR), and reevaluate BRET titration as a method to study membrane protein ass

147 Aside from acting as a reporter, BRET can also turn on functions in living systems.

148 and active H-Ras(G/V)-Venus exhibit a robust BRET signal at the plasma membrane that is markedly enha

149 4-Renilla luciferase (Rluc) exhibited robust BRET with the tethered GalphaiYFP, and this interaction

150 RTK BRET assays are highly sensitive for quantifying ligand-

151 RTK BRET-2 assays monitor, in living cells, the specific int

152 -terminus of GLUT1 and performing saturation BRET analysis, we were able to demonstrate the formation

153 should facilitate high-throughput sensitive BRET assays, including studies in single live cells and

154 the first time that an efficient sequential BRET-FRET energy transfer process based on firefly lucif

155 n the constitutive presence of a significant BRET signal above that in a series of controls, with thi

156 hough all constructs generated a significant BRET signal, this was disrupted by peptide in all except

157 A similar BRET(2)-based transduction scheme approach would likely

158 In addition, specific BRET signals were observed for AGS4-RLuc and alpha(2)-ad

159 reliably separates nonspecific and specific BRET.

160 otein (YFP) demonstrated saturable, specific BRET signals.

161 sence and absence of rapamycin, the specific BRET signal was determined.

162 ization induced by dopamine, with subsequent BRET signals increasing when luciferase8-tagged D2R appr

163 ed to an image splitter, we demonstrate that BRET can be used to image protein interactions in plant

164 Further, the fact that BRET changes at the Gbetagamma-Kir3 interface are predic

165 iple levels of donor expression we find that BRET between beta2AR protomers is directly proportional

166 The BRET assay, based on the interaction with Calmodulin, wa

167 The BRET signal between D2R-Luc and GFP2-K-Ras was robustly

168 The BRET technique is complementary to one based on FRET, de

169 The BRET technology provides an assay platform to study sign

170 Additionally, the BRET assay suggested that depletion of phosphorylation d

171 teins as the BRET donor, quantum dots as the BRET acceptor, and protease substrates sandwiched betwee

172 a fluorescently conjugated ASO acting as the BRET acceptor.

173 rs consist of bioluminescent proteins as the BRET donor, quantum dots as the BRET acceptor, and prote

174 g a Nano luciferase tagged PC4 acting as the BRET donor, to a fluorescently conjugated ASO acting as

175 We concluded the BRET assay is an accurate, sensitive, and cost/time effi

176 ) sensor, we found that GPR158 decreases the BRET signal as observed upon G-protein activation; howev

177 ontrast, rhodopsin was unable to disrupt the BRET signal, indicating that the disruption of the PAR4

178 cells with brefeldin A did not eliminate the BRET signals, and morphologic FRET experiments confirmed

179 anging from 10nM to 3.16 muM maltose for the BRET(2) system compared to an EC(50) of 2.3 muM and a li

180 However, the BRET vectors currently used lack adequate sensitivity fo

181 her, chemicals that screened positive in the BRET assay also stimulated phenotypic outcomes in daphni

182 BRET(2)) system showed a 30% increase in the BRET ratio upon maltose binding, compared with a 10% inc

183 shows an overall 5.5-fold improvement in the BRET ratio, thereby greatly enhancing the dynamic range

184 troduction of a single point mutation in the BRET(2) tagged MBP protein.

185 sites on surface enabled us to maximize the BRET efficiency by adjusting the QD/enzyme conjugation r

186 the absence of ligand, we have monitored the BRET signal after deletion of regions of the extracellul

187 The specificity of the BRET and FRET signals was confirmed by control studies.

188 nical advances to enhance the utility of the BRET assay in plants.

189 The magnitude of the BRET signal was normalized to the maximum response obtai

190 y greatly enhancing the dynamic range of the BRET signal.

191 In addition, the low background of the BRET system allowed detection of significant, but less e

192 similarly observed to have no effect on the BRET(2) signal.

193 Overall, the BRET assay had comparable or greater sensitivity as comp

194 Taken together, the BRET assays reported here could further help decipher D2

195 ) clock genes from cyanobacteria, we use the BRET technique to demonstrate that the clock protein Kai

196 Using the BRET-based biosensor, maltose in water was detected on a

197 rhodopsin are quantified and imaged with the BRET Ca(++) sensor in darkness, thereby avoiding undesir

198 gands in mice showed no correlation with the BRET data is consistent with the absence of association

199 These BRET systems consist of the recently developed Renilla r

200 We show the use of these BRET systems for ratiometric imaging of both cells in cu

201 We applied this BRET uncaging system to release a potent kinase inhibito

202 , bioluminescence resonance energy transfer (BRET(2)) analyses confirmed that the hLHR constitutively

203 g bioluminescence resonance energy transfer (BRET(2)), we demonstrated that CXCR4 multimers are found

204 a bioluminescence resonance energy transfer (BRET) and enabled the complex to emit NIR light.

205 TRF) assay, bioluminescence energy transfer (BRET) and Western blotting, while immunostainings and im

206 a bioluminescence resonance energy transfer (BRET) approach, we demonstrate that a series of antipsyc

207 d bioluminescence resonance energy transfer (BRET) assay platform, our studies in human embryonic kid

208 minescent Forster resonance energy transfer (BRET) assay using the luminescent donor Nanoluciferase a

209 d bioluminescence resonance energy transfer (BRET) assay, called type-4 BRET, which detects both homo

210 e bioluminescence resonance energy transfer (BRET) assays for monitoring the formation of ERalpha/bet

211 nd bioluminescent resonance energy transfer (BRET) assays in transfected cells, the present study att

212 y bioluminescence resonance energy transfer (BRET) assays of live cells.

213 d bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the pres

214 , bioluminescence resonance energy transfer (BRET) assays revealed that sustained activation by SNC-8

215 d bioluminescence resonance energy transfer (BRET) assays that measure relative distances between Ren

216 d bioluminescence resonance energy transfer (BRET) assays were developed to monitor the activation of

217 y bioluminescence resonance energy transfer (BRET) assays.

218 e bioluminescence resonance energy transfer (BRET) assays.

219 n bioluminescence resonance energy transfer (BRET) between JNK3-luciferase and Venus-arrestins.

220 f bioluminescence resonance energy transfer (BRET) between receptor constructs that included carboxyl

221 Bioluminescence resonance energy transfer (BRET) between Renilla luciferase and yellow fluorescent

222 y bioluminescence resonance energy transfer (BRET) between Renilla luciferase fused to the phosphatas

223 r bioluminescence resonance energy transfer (BRET) between RLuc8 and iRFPs, the chimeric luciferases

224 ed bioluminescent resonance energy transfer (BRET) biosensor, comprising maltose binding protein (MBP

225 , bioluminescence resonance energy transfer (BRET) biosensors, and the label-free approach surface pl

226 h bioluminescence resonance energy transfer (BRET) donor/acceptor pairs that allowed us to evaluate i

227 Bioluminescence resonance energy transfer (BRET) experiments revealed that DHHC2 or DHHC3 (Golgi-sp

228 Bioluminescence resonance energy transfer (BRET) has been widely used for studying dynamic processe

229 a bioluminescence resonance energy transfer (BRET) imaging approach that directly supports the measur

230 y bioluminescence resonance energy transfer (BRET) in human embryonic kidney 293 cells.

231 d bioluminescence resonance energy transfer (BRET) in live cells to identify a short motif in the C-t

232 g bioluminescence resonance energy transfer (BRET) in live cells, we show that RGS14-Luciferase and a

233 g bioluminescence resonance energy transfer (BRET) in live cells, we show that WNT5A stimulates dimer

234 n bioluminescence resonance energy transfer (BRET) interaction assays, all the truncated POU3F3 versi

235 Bioluminescence resonance energy transfer (BRET) is a natural biophysical phenomenon that underlies

236 Bioluminescence resonance energy transfer (BRET) is a sensitive optical detection method that can m

237 Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating pro

238 Bioluminescence resonance energy transfer (BRET) is currently used for monitoring various intracell

239 Bioluminescence resonance energy transfer (BRET) is often used to study association of membrane pro

240 t bioluminescence resonance energy transfer (BRET) occurred minimally in intact versions of these rec

241 Bioluminescence resonance energy transfer (BRET) operates with biochemical energy generated by biol

242 e bioluminescence resonance energy transfer (BRET) phenomenon, we report the development of a highly

243 A bioluminescence resonance energy transfer (BRET) readout of heterotrimer activation with high tempo

244 ) bioluminescence resonance energy transfer (BRET) reporters to monitor conformational changes in bet

245 a bioluminescence resonance energy transfer (BRET) sensor, we found that GPR158 decreases the BRET si

246 f bioluminescence resonance energy transfer (BRET) sensors, we demonstrate that RGSz1 modulates Galph

247 a bioluminescence resonance energy transfer (BRET) signal in the presence of a CoA-linked fluorophore

248 h bioluminescence resonance energy transfer (BRET) studies of liganded-beta2AR binding to arrestin an

249 d bioluminescence resonance energy transfer (BRET) studies on COS cells coexpressing MOP and CCK2 rec

250 d bioluminescence resonance energy transfer (BRET) studies.

251 a bioluminescence resonance energy transfer (BRET) system, and split reporter protein complementation

252 d bioluminescence resonance energy transfer (BRET) techniques, calcium flux measurements, and microsc

253 Bioluminescence resonance energy transfer (BRET) technology offers new insight by allowing the dire

254 e bioluminescence resonance energy transfer (BRET) technology to quantitatively study the pharmacolog

255 g bioluminescence resonance energy transfer (BRET) technology, they show that individual RAF family m

256 e bioluminescence resonance energy transfer (BRET) technology, which directly measures the recruitmen

257 g bioluminescence resonance energy transfer (BRET) technology.

258 n bioluminescence resonance energy transfer (BRET) that allow for assaying PPIs both in cell culture

259 n bioluminescence resonance energy transfer (BRET) that allows detection of antibodies directly in so

260 d bioluminescence resonance energy transfer (BRET) to demonstrate that the prototypic family B secret

261 d bioluminescence resonance energy transfer (BRET) to detect and quantify assembly of the methyl farn

262 s bioluminescence resonance energy transfer (BRET) to detect binding of CBT-labeled growth factors to

263 d bioluminescence resonance energy transfer (BRET) to examine oligomerization of Ste2p, a G protein-c

264 g bioluminescence resonance energy transfer (BRET) to reveal the binding characteristics of a drug wi

265 e bioluminescence resonance energy transfer (BRET) to show that after activation, Galphas rapidly ass

266 d bioluminescence resonance energy transfer (BRET) to study the Arf1/AP-1 interaction and AP-1 confor

267 Bioluminescence resonance energy transfer (BRET) was assessed in HEK293 cells expressing 5-HT(2C) r

268 d bioluminescence resonance energy transfer (BRET) were used to examine the PAR4 homodimer interface.

269 , bioluminescence resonance energy transfer (BRET), and functional analysis to map spatial approximat

270 , bioluminescence resonance energy transfer (BRET), avoids these problems because it uses enzyme-cata

271 g bioluminescence resonance energy transfer (BRET), HO-1 formed HO-1*P450 complexes with CYP1A2, CYP1

272 d bioluminescence resonance energy transfer (BRET), uses a bioluminescent luciferase that is genetica

273 g bioluminescence resonance energy transfer (BRET), we detected a constitutive and phorbol 12-myrista

274 Bioluminescence resonance energy transfer (BRET), which relies on nonradiative energy transfer betw

275 g bioluminescence resonance energy transfer (BRET)-an energy transfer process between light-emitting

276 g bioluminescence resonance energy transfer (BRET)-based and conformational fluorescein arsenical hai

277 a bioluminescence resonance energy transfer (BRET)-based assay.

278 n bioluminescence resonance energy transfer (BRET)-based assays to give an insight into the structure

279 n bioluminescence resonance energy transfer (BRET)-based assays, with high D(3)R affinities (K(i) = 0

280 r bioluminescence resonance energy transfer (BRET)-based biosensor, capable of detecting signal-depen

281 e bioluminescence resonance energy transfer (BRET)-based high-throughput screening (HTS) strategy to

282 y bioluminescence resonance energy transfer (BRET)-based saturation and kinetic binding experiments,

283 l bioluminescence resonance energy transfer (BRET)-fluorescence resonance energy transfer (FRET) proc

284 g bioluminescence resonance energy transfer (BRET).

285 g bioluminescence resonance energy transfer (BRET).

286 f bioluminescence resonance energy transfer (BRET).

287 y bioluminescence resonance energy transfer (BRET).

288 d bioluminescence resonance energy transfer (BRET-2) assays, diltiazem was a partial agonist at GHSR1

289 We have used BRET to study the in vivo activation of AP-1.

290 al approach to test this hypothesis, we used BRET to examine 7TM receptor-mediated regulation of Galp

291 Using BRET-based biosensors, we showed that whereas K17F activ

292 -protein interaction can be documented using BRET.

293 Finally we provide conclusive evidence using BRET and FRET that OXRs and GPR103 form functional heter

294 ions between CB1 and D2L were observed using BRET(2) Cotreatment of STHdh(Q7/Q7) cells with ACEA and

295 This work validates BRET as a powerful tool for interrogating and observing

296 beta-arrestin recruitment (as monitored via BRET assays).

297 assessing membrane protein association with BRET.

298 tor and GIP receptor were characterized with BRET donor saturation studies, shift experiments, and te

299 alpha(i1)-YFP and AGS4-Rluc-G-Galpha(i1)-YFP BRET were observed in both pellet and supernatant subcel

300 -Galpha(i1)-YFP and AGS4-Rluc-Galpha(i1)-YFP BRET were regulated by Ric-8A but not by Galpha-interact