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1 C. reinhardtii BBS4 may be required for the export of si
2 C. reinhardtii cells deprived of iron have more saturate
3 C. reinhardtii cells exposed to oxidative stress show in
4 C. reinhardtii has contributed to our understanding of c
5 C. reinhardtii insertional mutants defective in BBS1, -4
6 C. reinhardtii is serving as an important model organism
7 C. reinhardtii knockdown mutants for GPD2 and GPD3 showe
8 C. reinhardtii mutants null for cia5 do not express seve
9 ibodies raised to PsbW we have examined: (1) C. reinhardtii mutants lacking either photosystem and (2
12 scribe the cloning and characterization of a C. reinhardtii version of a TRP channel sharing key feat
13 for Rca was cloned and expressed in pSL18, a C. reinhardtii expression vector conferring paromomycin
15 Under nitrogen deprivation, the green alga C. reinhardtii showed substantial triacylglycerol (TAG)
16 th a polyacrylate coating) by the green alga C. reinhardtii was investigated in order to assess the c
17 ng the atypically fast IFT in the green alga C. reinhardtii, on average, 10 kinesin-2 motors "line up
19 he most thoroughly studied unicellular alga, C. reinhardtii, is the current standard for algal resear
21 ithii +, plus the new eastern North American C. reinhardtii isolates, comprise one morphological spec
22 ommon unicellular ancestor of V. carteri and C. reinhardtii and that this gene was lost in the latter
23 region with several predicted V. carteri and C. reinhardtii proteins and that this region, the VARL d
25 nce between cytochrome f of P. laminosum and C. reinhardtii (E(m7) = 297 and 370 mV, respectively).
30 of the wide array of experimental approaches C. reinhardtii offers, Lechtreck and Witman determined t
32 tion pathway in the dark, especially because C. reinhardtii PFR1 was also able to allow H(2) evolutio
33 response is physiologically relevant because C. reinhardtii experiences these growth conditions routi
34 nach and pea thioredoxin f, -300 mV for both C. reinhardtii and spinach thioredoxin m, -320 mV for sp
35 nd/or ASQD in photosynthesis as conducted by C. reinhardtii, particularly under phosphate-limited con
36 nic forms of Se, are readily internalized by C. reinhardtii, but selenite is accumulated around ten t
39 ST) evidence and annotation of the completed C. reinhardtii genome identified genes for each of the f
43 thway for Hyd1 expression in oxygen-depleted C. reinhardtii demonstrates the existence of multiple ox
44 ived sequences among nuclear genome data for C. reinhardtii, which also contrasts with the situation
47 ws for the generation of stable, marker-free C. reinhardtii transformants without the supplementation
48 ast FLA8/10 and slow KLP11/20 kinesin-2 from C. reinhardtii and C. elegans through a DNA tether to un
49 comparison of genomic Hind10 fragments from C. reinhardtii rs-3 and its wild-type progenitor CC-407
50 oxidant function, the nuclear VTE2 gene from C. reinhardtii was overexpressed in the npq1 lor1 double
54 ty in the spectra for WT PS I particles from C. reinhardtii and Synechocystis sp. 6803 indicates that
56 00(+) formation, in both PS I particles from C. reinhardtii, the higher-frequency carbonyl band upshi
57 Evidently, docking of these proteins from C. reinhardtii is due to hydrophobic interaction, slight
58 and by nuclear transformation with VTE2 from C. reinhardtii, which resulted in 1.6-fold, 5-fold to 10
59 he previously identified SUMO conjugase gene C. reinhardtii ubiquitin-conjugating enzyme9 (CrUBC9) is
64 signal for acclimation to limiting CO(2) in C. reinhardtii is unidentified, and it is not known how
67 cular and nonvascular plant databases and in C. reinhardtii but absent from cyanobacterial genomes.
68 ot detection of CSRA and CSRB apoproteins in C. reinhardtii cells enabling assessment of the cellular
72 rk provides insight into TAG biosynthesis in C. reinhardtii, and paves the way for engineering microa
73 cts with an antibody to lumen-directed CA in C. reinhardtii, and because it can be removed with 1 M C
77 abundance or transient regulatory complex in C. reinhardtii that may be similar to DREAM-like complex
78 of at least one additional SUMO conjugase in C. reinhardtii, a conjugase tentatively identified as Cr
79 istinct and functional SUMO E2 conjugases in C. reinhardtii, with a clear division of labor between t
81 the first large-scale collection of CREs in C. reinhardtii to facilitate further experimental study
82 regulatory function is wired differently in C. reinhardtii to control qE capacity via cis-regulatory
84 rafish embryos or mutation of TTC26/DYF13 in C. reinhardtii, produced short cilia with abnormal motil
85 h as much as approximately 10% efficiency in C. reinhardtii We demonstrate its use in transgene- and
87 ral dynamics of osmotic Ca(2+) elevations in C. reinhardtii suggest important mechanistic differences
88 y omega-3 fatty acid desaturase expressed in C. reinhardtii, and we discuss possible mechanisms of ho
90 known chloroplast ferredoxins (FDX1-FDX6) in C. reinhardtii, FDX1 and FDX2 were the most efficient el
93 ults suggest a mechanism for LD formation in C. reinhardtii involving chloroplast envelope membranes
96 he molecular cloning of the two Ppc genes in C. reinhardtii (CrPpc1, CrPpc2), each of which is transc
98 ed expression of the LHCSR and PSBS genes in C. reinhardtii The spa1 and cul4 mutants accumulate incr
99 nt high-level expression of foreign genes in C. reinhardtii, which has not previously been reliably a
102 We monitored the accumulation of GFP in C. reinhardtii chloroplasts transformed with the codon-o
103 s expression with the accumulation of GFP in C. reinhardtii transformed with a non-optimized GFP cass
111 identification at the whole genome level in C. reinhardtii using a comparative genomics-based method
112 protein phosphorylation under high light in C. reinhardtii, known to fully induce the expression of
115 sed a chloroplast luciferase gene, luxCt, in C. reinhardtii chloroplasts under the control of the ATP
116 gene family, which consists of 12 members in C. reinhardtii and 14 in V. carteri, has experienced a c
117 duced expression of Pcdp1 complex members in C. reinhardtii results in failure of the C1d central pai
119 tal validation of several novel microRNAs in C. reinhardtii that were predicted by miRvial but missed
121 vement of DNA from chloroplast to nucleus in C. reinhardtii, which may reflect the ultrastructure of
122 the transgene silencing that often occurs in C. reinhardtii, the FPs were expressed from the nuclear
124 complex antenna phosphorylation patterns in C. reinhardtii compared to Arabidopsis are discussed in
125 Artificial microRNA silencing of PDAT in C. reinhardtii alters the membrane lipid composition, re
127 panying software tool and the predictions in C. reinhardtii are also made available through a Web-acc
132 show that PsbS is a light-induced protein in C. reinhardtii, whose accumulation under high light is f
133 activation of non-photochemical quenching in C. reinhardtii, possibly by promoting conformational cha
135 vents in the global N starvation response in C. reinhardtii, starting within minutes with the upregul
138 on factor-evolved from its ancestral role in C. reinhardtii as a mating-type specifier, to become a d
140 als that the miRNA-mediated RNA silencing in C. reinhardtii differs from that of higher plants and in
144 rol of autophagy in response to ER stress in C. reinhardtii In close agreement, we also found that au
145 te that the rate of chlorophyll synthesis in C. reinhardtii is not directly controlled by the express
146 -mediated repression of protein synthesis in C. reinhardtii may involve alterations to the function/s
147 Functionality of the 5' UTRs was tested in C. reinhardtii chloroplasts using beta-glucuronidase rep
150 hat mastigonemes enhance flagellar thrust in C. reinhardtii, and so, their function still remains eni
151 ardtii Here we analyzed state transitions in C. reinhardtii mutants of two phosphatases, PROTEIN PHOS
152 tioned for mRNA stability and translation in C. reinhardtii chloroplasts while the more divergent C.
155 SUMO-conjugating enzyme (SCE) (E2, Ubc9) in C. reinhardtii was shown to be functional in an Escheric
156 unteracting phosphatase(s) remain unknown in C. reinhardtii Here we analyzed state transitions in C.
158 9 kDa in extracts from anaerobically induced C. reinhardtii cells, strongly suggesting that HydA2 enc
160 ed may vary in photosynthetic organisms like C. reinhardtii from anoxia to high light to limitations
161 phage library was demonstrated by using live C. reinhardtii cells to pan for VH H clones with specifi
163 utionary distance between algae and mammals, C. reinhardtii ATPase 6 functioned in human cells, becau
168 X proteins can participate in acclimation of C. reinhardtii cells to excess absorbed light energy.
169 ii Our results indicate that the activity of C. reinhardtii ATG4 is regulated by the formation of a s
172 lasticity of the photosynthetic apparatus of C. reinhardtii This alga is able to use various photoacc
173 w, using ODAs extracted from the axonemes of C. reinhardtii, that the C-terminal beta-propeller but n
175 on-dense vacuoles or polyphosphate bodies of C. reinhardtii showed large amounts of phosphorus, magne
178 nt results for deuterated wild-type cells of C. reinhardtii demonstrating that both radical pairs P70
179 ation of high light to dark-adapted cells of C. reinhardtii led to an increase in the amplitudes of 6
180 Therefore, in mixotrophically grown cells of C. reinhardtii, interpretations of the effects of enviro
182 ation in Escherichia coli by coexpression of C. reinhardtii HydEF and HydG and the HydA1 [FeFe] hydro
183 mentally measured steady-state Cd content of C. reinhardtii in the presence of low or high [Zn(2+)].
184 usly shown that when mixotrophic cultures of C. reinhardtii (which use both photosynthesis and mitoch
185 n the regulation of the sexual life cycle of C. reinhardtii, which is controlled by blue and red ligh
186 n important role in the sexual life cycle of C. reinhardtii: It controls the germination of the alga,
187 d on this we suggest that the development of C. reinhardtii as an industrial biotechnology platform c
188 n opportunity to expedite the development of C. reinhardtii as an industrial biotechnology platform,
189 e 251-residue extrinsic functional domain of C. reinhardtii cytochrome f was expressed in Escherichia
191 ent, indicated by 1) a consistent failure of C. reinhardtii vtc1 mutant strains, which are deficient
192 ed 309,278 raw EST sequencing trace files of C. reinhardtii and found that only 57% had cDNA termini
193 lustrate the marked metabolic flexibility of C. reinhardtii and contribute to the development of an i
199 he wild type (WT) and the HS(A676) mutant of C. reinhardtii indicates that the mutation primarily exe
200 isolation of a plasmid disruption mutant of C. reinhardtii, designated Deltasqd1, which lacks ASQD a
201 S I particles from a site-directed mutant of C. reinhardtii, in which the axial histidine ligand (His
202 Eight independently isolated mutants of C. reinhardtii that require high CO(2) for photoautotrop
204 e compared with two site-directed mutants of C. reinhardtii, in which the spin-polarized signal on ei
207 gene that is critical for the protection of C. reinhardtii from photo-oxidative damage under high li
208 Several of the large subunit proteins of C. reinhardtii have short extension or insertion sequenc
210 lly validated genome-scale reconstruction of C. reinhardtii metabolism that should serve as a useful
214 es of the different acclimation responses of C. reinhardtii and B. braunii Knowledge of the specific
218 gA gene, we analyzed the genome sequences of C. reinhardtii and V. carteri to identify additional gen
221 ned the high-resolution crystal structure of C. reinhardtii ODA16 (CrODA16) and mapped the binding to
222 tii and determined the crystal structures of C. reinhardtii IFT70/52 and Tetrahymena IFT52/46 subcomp
225 ble thylakoid membrane appression to that of C. reinhardtii at its optimal growth condition, UWO241 g
230 indicate that the electron-dense vacuoles of C. reinhardtii are very similar to acidocalcisomes with
232 with picosecond-fluorescence spectroscopy on C. reinhardtii cells that, although LHCs indeed detach f
233 s encoding the mouse homologues of the other C. reinhardtii C1d complex members are primarily express
234 component characteristic of photoautotrophic C. reinhardtii cultures grown at high light was not limi
235 gh TRP channels seem to be absent in plants, C. reinhardtii possesses genomic sequences encoding TRP
236 ansition from state 1 to state 2 can protect C. reinhardtii in high-light conditions and how this dif
238 with two cell types, one of which resembles C. reinhardtii cytologically but is terminally different
239 R sequences from four Chlamydomonas species (C. reinhardtii, C. incerta, C. moewusii and C. eugametos
246 , systems-level investigation indicated that C. reinhardtii cells sense and respond on a large scale
249 Chlamydomonas reinhardtii, and we show that C. reinhardtii PGK1 (CrPGK1) activity is inhibited by th
254 he possible interaction between CHT7 and the C. reinhardtii retinoblastoma tumor suppressor (RB) prot
255 of spinach chloroplast and E. coli, but the C. reinhardtii ribosome has proteins associated with the
259 els of recombinant protein expression in the C. reinhardtii chloroplast was due to the codon bias see
260 In this report we study the changes in the C. reinhardtii cyclophilin transcript and protein levels
261 abidopsis thaliana which is conserved in the C. reinhardtii enzyme, indicated localization in the pla
265 on by tunicamycin was more pronounced in the C. reinhardtii sor1 mutant, which shows increased expres
266 currently used to modify and interrogate the C. reinhardtii nuclear genome and explore several techno
268 lowed brilliant and specific staining of the C. reinhardtii cell wall and analysis of cell-wall genes
271 small subunit protein to the pyrenoid of the C. reinhardtii chloroplast in cells maintained under amb
279 of the ALS promoter with the promoter of the C. reinhardtii Rubisco small subunit gene (RbcS2) permit
281 e betaC-plastoglobuli proteome resembles the C. reinhardtii eyespot and Arabidopsis (Arabidopsis thal
283 , although developed for and tailored to the C. reinhardtii dataset, can be exploited by any eukaryot
286 essful complementation was achieved with the C. reinhardtii TLA2-CpFTSY gene, whose occurrence and fu
288 solated from the library show specificity to C. reinhardtii and lack of reactivity to antigens from f
290 ion of the chimeric gene using either of two C. reinhardtii chloroplast promoters and 5' and 3' RNA e
291 robically induced concomitantly with the two C. reinhardtii [Fe] hydrogenase genes, HydA1 and HydA2.
292 cation is present in the genome of wild-type C. reinhardtii but at a substantially lower level in a C
293 ses that accompany anaerobiosis in wild-type C. reinhardtii cells and a null mutant strain for the HY
300 -light (HL) photoacclimation strategies with C. reinhardtii and other frequently studied green algae: