ライフサイエンスコーパス: C2 domain

1 osolic region of E-Syts (i.e., the number of C2 domains).

2 also binds to the membrane through a cryptic C2 domain.

3 rm a Y-shaped complex, centered on the Vps34 C2 domain.

4 von Willebrand factor, and mediation by the C2 domain.

5 nd to several distinct surfaces on the FVIII-C2 domain.

6 sidue ordered region C-terminal to the VPS34 C2 domain.

7 ids and PTEN phospho-tail for binding to the C2 domain.

8 domain inhibitor antibodies and the isolated C2 domain.

9 cular interaction between its C-tail and the C2 domain.

10 pitopes on opposing faces of the factor VIII C2 domain.

11 1 domain by replacing it with the homologous C2 domain.

12 antibody inhibitors against the factor VIII C2 domain.

13 otyrosine-binding site within its N-terminal C2 domain.

14 develop antibodies against the fVIII A2 and C2 domains.

15 iated proteins that contain a tandem pair of C2 domains.

16 ature of the linker that connects its tandem C2 domains.

17 branes with antiparallel orientations of the C2 domains.

18 ain, and two conserved tandem Ca(2+)-binding C2 domains.

19 tructures show close contacts between A1 and C2 domains.

20 branes as the C2AB tandem than as individual C2 domains.

21 8I) to better occupy a cavity between A1 and C2 domains.

22 phatidylcholine, in the presence of the syt1 C2 domains.

23 the protein or via mutagenesis of its tandem C2 domains.

24 ns (cytoplasmic membrane) binding of its two C2 domains.

25 A (HA) are primarily directed to the A2 and C2 domains.

26 tate for Unc13 mediated by the tandem C1 and C2 domains.

27 ded with peptides spanning FVIII-A2, C1, and C2 domains.

28 pes of factor VIII (fVIII) are in the A2 and C2 domains.

29 mbranes are tightly tethered by Ca(2+)-bound C2 domains.

30 and alter the relative orientation of these C2-domains.

31 ansmembrane, linker, and two Ca(2+)-binding (C2) domains.

32 The C2 domain/3E6 FAB/G99 FAB ternary complex illustrates th

33 a classical calcium-dependent lipid binding C2 domain, a specific CAR signature is likely responsibl

34 ochondria by a mechanism that depends on its C2 domain, a unique Glu residue in its activation loop,

35 The C2-domain ABA-related (CAR) family of small proteins is

36 s are mediated through a 10-member family of C2-domain ABA-related (CAR) proteins in Arabidopsis thal

37 The C2-domain ABA-related (CAR) proteins play important role

38 t a membrane-binding defective mutant of the C2 domain abrogated these properties.

39 n of Munc13-1, or of Munc13-1 with a mutated C2 domain all disrupted L-type channel clustering at gra

40 Furthermore, we demonstrated that the C2 domain alone is capable of targeting PLC to the membr

41 disrupted the interaction between the tandem C2 domains, altered the intrinsic affinity of syt-1 for

42 dylinositol 3-phosphate (PtdIns(3)P) via its C2 domain, an association that may be required for endos

43 ombined data show anillin contains a cryptic C2 domain and a Rho-binding domain.

44 3E6 and G99 bind independently to the fVIII C2 domain and can form a stable ternary complex.

45 containing 1A (CC2D1A) gene, which encodes a C2 domain and DM14 domain-containing protein, has been l

46 es a truncated form of CC2D1A that lacks the C2 domain and three of the four DM14 domains, we show th

47 Multiple C2 domain and transmembrane region proteins (MCTPs) are

48 KY (QKY), a member of the family of multiple C2 domain and transmembrane region proteins (MCTPs), to

49 plicated noncatalytic regions, including the C2 domain and/or WW domain linkers in NEDD4-1 and WWP2,

50 min in which the Ca(2+)-binding sites in the C2 domains and a basic cluster involved in membrane bind

51 egion in C2A domain is longer than classical C2 domains and a novel Ca(2+) binding site in the vWA do

52 ease similar C-terminal modules, bearing two C2 domains and a transmembrane domain.

53 component to the interaction between ferlin C2 domains and lipid bilayers.

54 Cpd33 encodes a protein containing multiple C2 domains and transmembrane regions.

55 teins that bind the plasma membrane (PM) via C2 domains and transport lipids between them via SMP dom

56 Analysis of chimeric molecules, isolated C2 domains, and point mutants revealed that the C2B doma

57 attachment protein receptor), Synaptotagmin C2 domains, and the lipid bilayer in real time during th

58 Within the C2 domain, antibody epitope and kinetics are more import

59 ent conformations (all of its domains except C2 domain are visible).

60 domains of synaptotagmins, the Rasal tandem C2 domains are able to sense and induce membrane curvatu

61 malian cells and showed that while wild-type C2 domains are efficiently secreted, the mutant p.D871N

62 Proteins that contain C2 domains are involved in a variety of biological proce

63 lution binding affinity does not change when C2 domains are linked.

64 Ca(2+)-responsive C2 domains are peripheral membrane modules that target t

65 Syt-1 C2 domains are reported to bind membranes cooperatively,

66 Proteins containing C2 domains are the sensors for Ca(2+) and PI(4,5)P2 in a

67 C2 domains are widespread motifs that often serve as Ca(

68 and C2E domains and, to a lesser extent, the C2D domain are dispensable for dysferlin membrane repair

69 epitopes are on opposing sides of the fVIII C2 domain, are consistent with the solvent accessibility

70 In full-length Syt1, both Pb(2+)-complexed C2 domains associate with phosphatidylserine-containing

71 The 3E6 epitope forms direct contacts to the C2 domain at 2 loops consisting of Glu2181-Ala2188 and T

72 ns four DM14 domains at the N terminus and a C2 domain at the C terminus.

73 his interaction was facilitated by a cryptic C2 domain at the extreme N terminus of Psd2p (C2-1) as w

74 tanding of Notch signalling by identifying a C2 domain at the N-terminus of Notch ligands, which has

75 CAPS dimer formation required its C2 domain based on mutation or deletion studies.

76 ariations depending on the properties of the C2 domain-bearing protein, shedding light to understand

77 common ancestor (LECA) contained at least 10 C2 domains belonging to 6 well-defined families.

78 ally, sedimentation assays suggest all seven C2 domains bind lipid membranes, and that Ca(2+) enhance

79 Usually C2 domains bind membrane lipids, but that of PLCgamma2 d

80 Both the phosphatase and C2 domains bind phosphatidylserine lipids, which likely

81 Under resting conditions, the Syt1 C2 domains bind the membrane with a magnesium (Mg(2+))-m

82 Their respective C2 domains bind via 2 pairs of hydrophobic amino acids a

83 Taken together, these data suggest that C2 domain binding to membranes is an important element i

84 ined in flexible top-loop sequences of their C2 domains--blocked the ability of these synaptotagmins

85 The C2 domains bracket a predicted SNARE-binding domain, but

86 ase by virtue of Ca(2+)-binding to their two C2 domains, but their mechanisms of action remain unclea

87 mechanism of Ca(2+) and lipid binding to the C2 domain by NMR spectroscopy and x-ray crystallography.

88 ated the apparent synergy between the tandem C2 domains by altering the length and rigidity of the li

89 t containing a disulfide bond between A1 and C2 domains by mutating Arg-121 and Leu-2302 to Cys (R121

90 es by IHCs is controlled by otoferlin, a six-C2-domain (C2-ABCDEF) protein that functions as a high-a

91 The copine protein is composed of two C2 domains (C2A and C2B) followed by a vWA domain.

92 Here we examine the effect of the two C2 domains (C2A and C2B) of syt1 on membrane lipid order

93 Munc13-4 contains N- and C-terminal C2 domains (C2A and C2B) predicted to bind Ca(2+), but C

94 inker region, and contains tandem C-terminal C2 domains (C2A and C2B) with affinity for phosphatidyli

95 ngle transmembrane helix (TM) and two tandem C2 domains (C2A and C2B).

96 ne protein whose phospholipid-binding tandem C2 domains, C2A and C2B, act as Ca(2+) sensors of neurot

97 ptotagmin-7 (Syt-7) contain analogous tandem C2 domains, C2A and C2B, which together sense Ca(2+) to

98 ), is largely composed of two Ca(2+)-sensing C2 domains, C2A and C2B.

99 for SV release that binds Ca(2+) via tandem C2-domains, C2A and C2B.

100 nd a fragment containing the synaptotagmin-1 C2 domains (C2AB) to membrane-anchored SNARE complex.

101 PLA(2)alpha truncated at the calcium-binding C2 domain (C2D).

102 The dual C2 domain Ca(2+)-binding mutant also enhanced spontaneou

103 This process requires otoferlin, a six C2-domain, Ca(2+)-binding transmembrane protein of synap

104 ring the plasma membrane-sensing role of the C2 domain, causes phorbol ester-triggered redistribution

105 requires the binding of Ca(2+) to two tandem C2 domains, CBD1 and CBD2, which are an integral part of

106 Calcium-binding C2 domain cells lost the ability of membrane translocati

107 n C2 domains of dysferlin as well as a multi-C2 domain construct.

108 luorescence measurements indicate that multi-C2 domain constructs of myoferlin, dysferlin, and otofer

109 variants in unrelated probands: WWC1 (WW and C2 domain containing 1), CELSR3 (Cadherin EGF LAG seven-

110 Coiled-coil and C2 domain containing 1A (CC2D1A) is an evolutionarily co

111 oforms (Syt2, Syt7, Syt9) as well as related C2 domain containing protein, Doc2B and extended Synapto

112 g an increase in accessibility of the nearby C2 domain containing the NLS.

113 asts lacking one of these gene products, the C2-domain containing protein, SMURF1, are deficient in t

114 Mutation of the coiled-coil and C2 domain-containing 1A (CC2D1A) gene, which encodes a C

115 Mutations in otoferlin, a C2 domain-containing ferlin family protein, cause non-sy

116 ntification of an IRT1 inhibitor, namely the C2 domain-containing peripheral membrane protein ENHANCE

117 ss of the signaling scaffold coiled-coil and C2 domain-containing protein 1A (CC2D1A), which is mutat

118 We have recently demonstrated that a novel C2 domain-containing protein kinase, EhC2PK is involved

119 Several C2 domain-containing proteins are known to regulate Ca(2

120 by screening all ( approximately 139) human C2 domain-containing proteins by RNA interference in neu

121 Several C2 domain-containing proteins, such as protein kinase C

122 The molecular events by which each otoferlin C2 domain contributes to the regulation of the synaptic

123 hat the C1 domain, in addition to the A2 and C2 domains, contributes significantly to the humoral ant

124 xplore the molecular mechanisms by which the C2 domain controls the initial step in the activation of

125 asured during confinement, we find that both C2 domains cooperate in membrane binding, with the C2B d

126 t participate in such tethering function via C2 domain-dependent interactions with the PM that requir

127 We constructed a FVIII variant lacking the C2 domain (designated DeltaC2) to characterize the contr

128 We found that mutations of aspartates in the C2 domains did not alter plasma membrane localization bu

129 senses Ca(2+); moreover, the tethered tandem C2 domains display properties distinct from the isolated

130 binding results in a drastic movement of the C2 domain, disrupting the intrinsic PRC2 dimer.

131 oteins in the synaptotagmin (syt) and double C2 domain (Doc2) families regulate exocytotic membrane f

132 Yao et al. now report that double C2 domain (Doc2) proteins function as high-affinity Ca(2

133 ns within the soluble calcium-binding double C2 domain (Doc2)-like protein family to selectively redu

134 membrane docking geometries of the PKCalpha C2 domain docked to (i) PS alone and (ii) both PS and PI

135 plex suggests that antibody 3E6 recognizes a C2 domain epitope consisting of the Arg(2209)-Ser(2216)

136 no detectable internal rearrangement of its C2 domains, even as it rapidly inserts into the bilayer.

137 Here, we first determined whether these C2-domains execute distinct functions.

138 In particular, the soluble C2-domain factor Doc2b plays a key role in GLUT4 vesicle

139 ations that disrupted Ca(2+) binding to both C2 domains failed to rescue evoked release, but supporte

140 C1 domains, indicating that the A1, A2, and C2 domains fold independently into antigenically intact

141 in constructs, each lacking one of the seven C2 domains, for their ability to localize to the plasma

142 We demonstrated previously that the C2 domain from PKCalpha (C2alpha) binds Pb(2+) with high

143 C2 domain from protein kinase Calpha (C2alpha) was chose

144 ce that Munc13-4 with its two Ca(2+)-binding C2 domains functions as a Ca(2+) sensor for SG exocytosi

145 ough located distant to the active site, the C2 domain greatly enhances catalytic turnover.

146 additional regulatory protein, Doc2b (double C2 domain), has recently been implicated in exocytosis f

147 For Syt1, the two Ca(2+)-saturated C2 domains have similar affinities for membranes lacking

148 We prepared mutants in which the C2 domain hydrophobic amino acid pairs were changed to t

149 e structures of the loops at the apex of the C2 domain implicated in membrane recognition and Jagged1

150 rystallographic structure of the factor VIII C2 domain in complex with 2 antibodies that illuminates

151 n astrocytes triggers the interaction of the C2 domain in cytosolic phospholipase A2 (cPLA2) with the

152 These data underscore the key role of the C2 domain in driving conventional PKC isozymes to the pl

153 g proteins EHD1 and EHD2 and that the second C2 domain in Fer1L5 mediates this interaction.

154 erve clustering of anionic lipids around the C2 domain in preference to the phosphatase domain, sugge

155 eate the unique and shared functions of each C2 domain in regulation of synaptic vesicle fusion.

156 y restricted loop regions and positioning of C2 domains in close proximity to anionic lipid headgroup

157 inding protein (SMP) domain followed by five C2 domains in E-Syt1 and three C2 domains in E-Syt2/3.

158 lowed by five C2 domains in E-Syt1 and three C2 domains in E-Syt2/3.

159 We expressed wild-type and mutant alpha2(VI) C2 domains in mammalian cells and showed that while wild

160 shown that EHD proteins bind directly to the C2 domains in myoferlin, a protein that regulates myobla

161 be a key determinant of the functions of Syt C2 domains in neurotransmitter release.

162 opological similarity to an integral fold of C2 domains, including a putative basic binding pocket.

163 ch more slowly than either of its individual C2 domains, indicating cooperativity, whereas the major

164 Similarly, mAb ESH4 against the C2 domain, inhibited >90% of platelet-dependent fVIII ac

165 lonal antibody (mAb) ESH8, against the fVIII C2 domain, inhibited binding of fVIII to SF and platelet

166 ctions between two different classes of anti-C2 domain inhibitor antibodies and the isolated C2 domai

167 UE domains and that its association with the C2 domain inhibits PtdIns(3)P binding.

168 asurements indicate that all seven dysferlin C2 domains interact with Ca(2+) with a wide range of bin

169 ntaxin 4 and vinculin, and that the putative C2 domains interact with lipid membranes.

170 he absence of calcium, consistent with intra-C2 domain interactions forming a "closed" tertiary struc

171 opy, we found that the synergy between these C2 domains involved intra-molecular interactions between

172 The factor VIII C2 domain is a highly immunogenic domain, whereby inhibi

173 The C2 domain is a useful model to understand these events b

174 over effects observed when the extracellular C2 domain is included.

175 the phosphatase domain, suggesting that the C2 domain is involved in nonspecific interactions with n

176 s unknown if Ca(2+) interactions with either C2 domain is required for suppression of asynchronous re

177 ion to Ca2+ influx, but Ca2+ binding by both C2 domains is required to flip the electrostatic switch

178 ith the p.VYEEDM1161V indel suggest that the C2 domain may have a role in regulating PLCgamma1 activi

179 rol in Arabidopsis, demonstrating that these C2 domains may be cooperative to mediate FTIP1 function

180 rt, we demonstrate that calcium releases the C2 domain-mediated auto-inhibition in both Nedd4-1 and N

181 nally, previously described Ca(2+)-dependent C2 domain-mediated autoinhibition of Nedd4-2 is not obse

182 We conclude that CAPS functions as a C2 domain-mediated dimer in regulated vesicle exocytosis

183 Following this C2 domain-mediated membrane recruitment, the C1 domain b

184 Ferlins are large multi-C2 domain membrane proteins involved in membrane fusion

185 Expression of the PTEN C2 domain mimicked effects of full-length PTEN but a mem

186 and analyzed Pten knock-in mice harboring a C2 domain missense mutation at phenylalanine 341 (Pten(F

187 dynamic folding options; 3) C-terminal four-C2 domain module; and 4) calpain-cleaved mini-dysferlinC

188 ction of the SYT tubes shows that one of the C2 domains (most likely C2B, based on its biochemical pr

189 C2 domain mutants (N345K and C420R) also mimic these eve

190 Moreover, C2 domain mutations or deletions resulted in a loss of C

191 While two other distinct families of C2 domains, namely those in PI3K-C2 and PTEN-C2 are also

192 o alternative splicing to include DBL/PH and C2 domains not present in invertebrate Itsn proteins.

193 ween otoferlin C2F domain and intramolecular C2 domains occurred in the absence of calcium, consisten

194 ry complex, showing an angle centered at the C2 domain of approximately 130 degrees .

195 The C2 domain of fVIII is a significant antigenic target of

196 ssion of the II-III loop of the channel, the C2 domain of Munc13-1, or of Munc13-1 with a mutated C2

197 The C2 domain of NEDD4-1 is required for its interaction wit

198 The Tyr(P) peptide also binds the C2 domain of PKCdelta tightly, but no enzyme activation

199 We previously identified the amino-terminal C2 domain of PKCtheta as a phosphotyrosine (pTyr)-bindin

200 Collectively, these studies establish the C2 domain of PKCtheta as a Tyr(P)-binding domain and sug

201 taining peptide derived from CDCP1 binds the C2 domain of PKCtheta with high affinity and activates t

202 The identified motifs are located in the C2 domain of plant PLCs and are not found in any other p

203 Overall, these data suggest that the C2 domain of PLC plays a vital role in calcium signallin

204 ex is controlled by the weakly characterized C2 domain of PLCgamma2.

205 lly, it is the first mutation located in the C2 domain of PLCZ1, a domain involved in targeting prote

206 ltiscale metal-ion-dependent dynamics of the C2 domain of protein kinase Calpha (C2alpha) using NMR s

207 (C2-1) as well the previously characterized C2 domain of Psd2p (C2-2).

208 tB2p/Pdr17p; a PtdIns 4-kinase, Stt4p; and a C2 domain of Psd2p.

209 We demonstrate that the non-catalytic C2 domain of PTEN specifically binds PI(3)P through the

210 main swapping involving RBBP4 and the unique C2 domain of SUZ12.

211 binding domain of MAP4 binds directly to the C2 domain of the p110alpha catalytic subunit.

212 and membrane binding properties of all seven C2 domains of dysferlin as well as a multi-C2 domain con

213 Of the five C2 domains of E-Syt1, only C2A and C2C contain Ca(2+)-bi

214 e (EGF) domains 8 and 12 engage the EGF3 and C2 domains of Jag1, respectively, and different Notch1 d

215 m coordinating residues in each of the three C2 domains of MCTP.

216 inactivation, confirming that the C-terminal C2 domains of otoferlin are essential for normal gating

217 roteins, we demonstrate that five of the six C2 domains of otoferlin sense calcium with apparent diss

218 we investigate the effects of binding of the C2 domains of otoferlin, dysferlin, and myoferlin on the

219 an acceptor membrane complex containing the C2 domains of Psd2p, PstB2p, and Pbi1p that ligate to Sc

220 ration encoded regions of the phosphatase or C2 domains of PTEN.

221 Ca-dependent interaction between the tandem C2 domains of Rasal and lipids of the membrane is also r

222 e of interfacial residues between the tandem C2 domains of synaptotagmin (syt)-1, a Ca(2+) sensor for

223 Finally we show, that similar to the C2 domains of synaptotagmins, the Rasal tandem C2 domain

224 These results suggest that the tandem C2 domains of Syt 1 play independent roles in neurotrans

225 hat physical interactions between the tandem C2 domains of syt-1 contribute to excitation-secretion c

226 ntrast, we previously suggested that the two C2 domains of Syt-7 bind membranes independently, based

227 ulated by Syt1 multimerization and that both C2 domains of Syt1 are uniquely required for modulating

228 Notably, either of the C2 domains of syt1, C2A or C2B, was able to function as

229 The two C2 domains of Syt1, which may mediate fusion by bridging

230 s used to estimate distances between the two C2 domains of syt1.

231 The tandem C2-domains of synaptotagmin 1 (syt) function as Ca(2+)-b

232 ly interacted with the lipid-binding domain (C2 domain) of PTEN and sequestered it in the nucleus.

233 Our identification of novel C2 domains offers new insights into interaction between

234 suggest that the effects of each subsequent C2 domain on lipid ordering appear to be additive.

235 We further analyze in vivo effects of three C2 domains on the regulatory role of MCTP1 (FTIP1) in fl

236 (PIP2) complexes, revealing how Rabphilin-3A C2 domains operate in cooperation with PIP2/Ca(2+) and S

237 Syt 1 transgenes containing only individual C2 domains, or dual C2A-C2A or C2B-C2B chimeras, failed

238 Beyond membrane insertion by the C2 domains, other requirements for Synaptotagmin activit

239 nase Calpha (PKCalpha) possesses a conserved C2 domain (PKCalpha C2 domain) that acts as a Ca(2+)-reg

240 in VWF binding, and suggest that the A3 and C2 domains play ancillary roles in this interaction.

241 efficiently drove exocytosis when its tandem C2-domains pointed in the same direction.

242 is involved in ring formation, and the other C2 domain points radially outward.

243 ns and antibody binding to human A2, C1, and C2 domains presented as human serum albumin (HSA) fusion

244 In contrast, antibody G99 recognizes the C2 domain primarily through the Pro(2221)-Trp(2229) loop

245 on blocks Cdc15 binding to paxillin Pxl1 and C2 domain protein Fic1 and enhances Cdc15 dynamics.

246 Double C2-domain protein (Doc2) is a Ca(2+)-binding protein imp

247 ns Merlin and Expanded (Ex), and the WW- and C2-domain protein Kibra.

248 IGNIFICANCE STATEMENT Otoferlin, a large six-C2-domain protein, is essential for synaptic vesicle exo

249 Tandem C2 domain proteins in the synaptotagmin (syt) and double

250 that block PHP we identified mctp (Multiple C2 Domain Proteins with Two Transmembrane Regions).

251 eral and conserved structural aspect of many C2 domain proteins, including Synaptotagmins.

252 ory factors such as Sec1/Munc18 (SM), double C2-domain proteins (DOC2), and additional interacting pr

253 and beta (Doc2alpha and Doc2beta) are tandem C2-domain proteins proposed to function as Ca(2+) sensor

254 Most studies have concentrated on C2 domains prototyped by those in protein kinase C (PKC-

255 in primarily through its C1 domain, with the C2 domain providing a secondary attachment site.

256 Collectively, these studies identify a C2 domain-pTyr(313) docking interaction that controls AT

257 e changes in the relative disposition of the C2-domains result from changing the length of the poly-p

258 d that binding of one divalent metal ion per C2 domain results in loss of conformational plasticity o

259 Here we demonstrate that each of its C2 domains senses Ca(2+); moreover, the tethered tandem

260 ue structure of dysferlin, with seven tandem C2 domains separated by linkers, suggests dysferlin may

261 Disruption of Ca(2+) binding to the PKCbeta C2 domain specifically prevents PTP without impairing ot

262 notype combined with similar findings in the C2 domain stress the importance of inhibitor properties

263 ure of evolutionary relationships within the C2 domain superfamily is lacking.

264 ting a calcium-mediated stabilization of the C2 domain.t-SNARE complex.

265 Ca(2+)-dependent conserved-region 2 (C2) domains target their host signaling proteins to anio

266 Syt1 contains two cytoplasmic C2 domains, termed C2A and C2B, which coordinate Ca(2+)

267 hinge region and its phosphotyrosine-binding C2 domain that controls PKCdelta's enzymology indirectly

268 eakest affinity site triggers changes in the C2 domain that facilitate its interaction with lipid mem

269 nvestigated the properties of its N-terminal C2 domain that functions as an autoinhibitory domain.

270 to identify the conserved amino acids of the C2 domain that regulate the targeting of PLCZ1 and its s

271 delta structure reveals an unexpected second C2 domain that was previously unrecognized from sequence

272 membrane domain, two DysF domains, and seven C2 domains that mediate lipid- and protein-binding inter

273 13 and Tctex-1 (dynein light chain), and two C2-domains that bind to phospholipids, Ca(2+) and SNAREs

274 a) possesses a conserved C2 domain (PKCalpha C2 domain) that acts as a Ca(2+)-regulated membrane targ

275 in neurotransmitter release through its two C2 domains (the C2A and C2B domain).

276 Synaptotagmins bind Ca(2+) via two C2 domains, the C2A and C2B domains.

277 hrough binding of high local Ca(2+) to their C2 domains, the proteins that sense smaller global Ca(2+

278 hat are able to inhibit binding of the FVIII C2 domain to a model membrane by application of a combin

279 Comparison of the CAPS C2 domain to a structurally defined Munc13-1 C2A domain

280 he major driving force in the binding of the C2 domain to anionic membranes, whereas electrostatic in

281 tion by promoting anchoring of the PLCgamma2 C2 domain to phospho-SLP65.

282 Calcium disrupts binding of the C2 domain to the HECT domain.

283 sozymes is initiated by the binding of their C2 domains to membranes in response to elevations in int

284 led a correlation between the ability of the C2 domains to penetrate membranes in response to Ca(2+)

285 The binding of the C2 domains to phospholipid (PSF) has been modeled and pr

286 ral explanation for the ability of different C2 domains to pull plasma and vesicle membranes close to

287 differentially regulate binding of otoferlin C2 domains to target SNARE (t-SNARE) proteins and phosph

288 r Synaptotagmin binds Ca(2+) through its two C2 domains to trigger membrane interactions.

289 Otoferlin, a six-C2 domain transmembrane protein linked to deafness in hu

290 Structurally, SMURF1 consists of a C2 domain, two WW domain repeats, and a catalytic HECT d

291 s from a change in the hydrophobicity of the C2 domain upon Ca(2)(+) binding.

292 yses of intrinsic Ca(2+)-binding to the Syt7 C2 domains using isothermal titration calorimetry, did n

293 We study the diffusion of membrane-targeting C2 domains using single-molecule tracking in supported l

294 ersely, an RCP mutant lacking the PA-binding C2 domain was not capable of being tethered at pseudopod

295 alues in the tens of micromolar, whereas the C2D domain was least sensitive, with a near millimolar K

296 Finally, transfection of the PLA(2)IValpha C2 domain (which is directly involved in PLA(2)IValpha m

297 s to the kinase domain of PIPKIgamma via its C2 domain while Lysine 255 in PIPKIgamma acts as the maj

298 t step in this process is interaction of its C2 domain with target cell membranes, which is a calcium

299 f its N-terminal immunoglobulin (Ig)-like C0-C2 domains with actin and/or myosin.

300 soforms containing common C-terminal PDZ and C2 domains with homology to vertebrate active zone prote