ライフサイエンスコーパス: CAF-1

1 CAF-1 and each of its individual subunits stably bound t

2 CAF-1 and the Hir proteins operate in distinct but funct

3 CAF-1 binds histones H3 and H4 and deposits histones ont

4 CAF-1 depletion led to S-phase accumulation, increased h

5 CAF-1 does not protect the genome by assembling it into

6 CAF-1 is also involved in coordinating inheritance of st

7 CAF-1 is essential in human cells for the de novo deposi

8 CAF-1 loss immediately slows down DNA replication speed

9 CAF-1 was a substrate for DNA-dependent protein kinase,

10 CAF-1, Hir proteins, and Asf1 are histone H3/H4 binding

11 CAF-1-depleted cell extracts completely lacked DNA repli

12 CAF-1-mediated resistance to DNA damage is dependent on

13 Chromatin Assembly Complex 1 (CAF-1) is a conserved histone chaperone that deposits (H

14 pletion of the chromatin assembly complex-1 (CAF-1) complex, a histone chaperone that is required for

15 Chromatin assembly factor 1 (CAF-1) and Rtt106 participate in the deposition of newly

16 somatic cells, chromatin assembly factor 1 (CAF-1) appears to be a key player in assembling new nucl

17 Chromatin assembly factor 1 (CAF-1) assembles nucleosomes in a replication-dependent

18 on 1 (Asf1) and Chromatin Assembly Factor 1 (CAF-1) chaperone histones H3/H4 during the assembly of n

19 perone known as chromatin assembly factor 1 (CAF-1) contributes to transcriptional silencing in yeast

20 he heterotrimer chromatin assembly factor 1 (CAF-1) couples DNA replication to histone deposition in

21 Chromatin assembly factor 1 (CAF-1) deposits histones during DNA synthesis.

22 Chromatin assembly factor 1 (CAF-1) deposits histones H3 and H4 rapidly behind replic

23 stone chaperone Chromatin Assembly Factor 1 (CAF-1) deposits tetrameric (H3/H4)2 histones onto newly-

24 stone chaperone chromatin assembly factor 1 (CAF-1) deposits two nascent histone H3/H4 dimers onto ne

25 n 1 (Asf1p) and chromatin assembly factor 1 (CAF-1) in global transcriptional regulation in budding y

26 n 1 (Asf1p) and chromatin assembly factor 1 (CAF-1) in vivo.

27 Chromatin assembly factor 1 (CAF-1) is a H3-H4 histone chaperone that associates with

28 Chromatin assembly factor 1 (CAF-1) is a highly conserved protein that functions in D

29 Chromatin assembly factor 1 (CAF-1) is a histone H3-H4 chaperone that deposits newly

30 Chromatin assembly factor 1 (CAF-1) is required for inheritance of epigenetically det

31 Chromatin assembly factor 1 (CAF-1) is the histone chaperone responsible for histone

32 stone chaperone chromatin assembly factor 1 (CAF-1) mediates histone H3-H4 assembly during DNA replic

33 degradation of chromatin assembly factor 1 (CAF-1), a key player in de novo chromatin assembly, with

34 tor 1 (Asf1) to chromatin assembly factor 1 (CAF-1), another histone chaperone that is critical for t

35 ubunit of yeast chromatin assembly factor 1 (CAF-1), as well as upon the karyopherin Kap123p, but was

36 p150 subunit of chromatin assembly factor 1 (CAF-1), forming a multiprotein complex that also contain

37 Chromatin assembly factor 1 (CAF-1), one such protein, is a histone chaperone that de

38 stone chaperone Chromatin Assembly Factor 1 (CAF-1), which is recruited to DNA replication forks thro

39 bly mediated by CHROMATIN ASSEMBLY FACTOR 1 (CAF-1).

40 arge subunit of chromatin assembly factor 1 (CAF-1).

41 n cells lacking chromatin assembly factor 1 (CAF-1).

42 ing mediated by chromatin assembly factor 1 (CAF-1).

43 ysfunctional in chromatin assembly factor-1 (CAF-1) (fas1 and fas2 mutants), which are known to have

44 exes, including chromatin assembly factor-1 (CAF-1) and the Hir proteins .

45 Subunits of the chromatin assembly factor-1 (CAF-1) complex, including Chaf1a and Chaf1b, emerged as

46 The chromatin assembly factor-1 (CAF-1) is a histone chaperone that regulates chromatin a

47 Chromatin assembly factor-1 (CAF-1) is a three-subunit protein complex conserved thro

48 for subsequent chromatin assembly factor-1 (CAF-1)-dependent nucleosome assembly.

49 replication by chromatin assembly factor-1 (CAF-1).

50 SD-mediated repression and binding to KAP-1, CAF-1 p150, and SP100 but not LBR.

51 ubunit of human chromatin assembly factor 1; CAF-1) bind directly to helix 1 of histone H4, a region

52 he histone acetylation may be required for a CAF-1 independent pathway or function after deposition,

53 reas H3.1 is assembled into nucleosomes in a CAF-1-dependent reaction.

54 Yet direct knowledge about CAF-1's histone binding mode and nucleosome assembly pro

55 chromatin assembly factors (CAFs), Asf1 and CAF-1, in turning off the DNA damage checkpoint in buddi

56 in histone modifications linked to ASF1 and CAF-1-dependent pathways, including SAS-I- and Rtt109p-d

57 l30 mutants with defects linked to ASF1- and CAF-1-dependent pathways.

58 to the chromatin assembly factors Asf1p and CAF-1, we found pol30 mutants did not exhibit a gross de

59 his interaction suggests a role for MBD1 and CAF-1 p150 in methylation-mediated transcriptional repre

60 to investigate interactions between MMR and CAF-1- and ASF1A-H3-H4-dependent histone (H3-H4)2 tetram

61 ulation might be more complex; MutSalpha and CAF-1 interact not only with PCNA, but also with each ot

62 Notably, the VEX2-orthologue and CAF-1 in mammals are also implicated in exclusion and in

63 We suggest that PCNA and CAF-1 connect DNA replication to chromatin assembly and

64 The Hir proteins and CAF-1 share a common partner, the highly conserved histo

65 We found that SPT6 and CAF-1 perturbations required cell division to induce dif

66 mispair-containing DNA by the MMR system and CAF-1-dependent packaging of the newly replicated DNA in

67 omatin maturation in both wild-type (WT) and CAF-1 mutant yeast cells.

68 p123p, but was independent of Cac2p, another CAF-1 component, and other chromatin assembly proteins (

69 hanism of histone H3/H4 transfer among Asf1, CAF-1 and DNA from a thermodynamic perspective, we devel

70 During S phase the histone chaperones Asf1, CAF-1, and Rtt106 coordinate to deposit newly synthesize

71 ng strand ssDNA gaps interfere with the ASF1-CAF-1 nucleosome assembly pathway, and drive fork degrad

72 The interaction between CAF-1 and PCNA is essential for proper nucleosome assemb

73 hir1Delta double mutant cells that lack both CAF-1 and HIR complexes than in either single mutant.

74 bstantially defective in the absence of both CAF-1 and Asf1, whereas deleting CAC1 or ASF1 individual

75 Optimal modulation of both CAF-1 and transcription factor levels increased reprogra

76 his complex stimulates histone deposition by CAF-1 .

77 We further reveal that histone deposition by CAF-1 is required for efficient H3K9me2 enrichment at re

78 olecular mechanism for histone deposition by CAF-1, a reaction that has remained elusive for other hi

79 TAD5 and subsequent nucleosome deposition by CAF-1.

80 tion-coupled nucleosome assembly mediated by CAF-1 and Rtt106.

81 o the DNA-associated tetramer is promoted by CAF-1-induced H3/H4 oligomerization.

82 l )-dependent MMR reactions is suppressed by CAF-1- and ASF1A-H3-H4-dependent deposition of the histo

83 hile studies of the Saccharomyces cerevisiae CAF-1 complex enabled to propose a model for the histone

84 ent Rad21, and the histone chaperone CHAF1A (CAF-1 p150).

85 Mutants in the H3.1 chaperone CAF-1 (fas1-4) maintain a pattern similar to that of wil

86 3.3 chaperone HIRA, while the H3.1 chaperone CAF-1 becomes essential.

87 H3K56 in vivo, whereas the histone chaperone CAF-1 (chromatin assembly factor 1) in humans and Caf1 i

88 reening, we identified the histone chaperone CAF-1 as a critical component for Cd4 repression.

89 the evolutionary conserved histone chaperone CAF-1 for which the links between structure and function

90 molecules are assembled by histone chaperone CAF-1 in a replication-coupled process, whereas H3.3-H4

91 between histone H3-H4 with histone chaperone CAF-1 or Rtt106 increases in cells lacking Rad17.

92 ation, we demonstrate that histone chaperone CAF-1 reduces nascent chromatin accessibility by filling

93 r, our findings reveal the histone chaperone CAF-1 to be a novel regulator of somatic cell identity d

94 previously shown that the histone chaperone CAF-1's nucleosome deposition function is vital to preve

95 s from inactivation of the histone chaperone CAF-1.

96 se data indicate that the histone chaperones CAF-1 and Asf1p regulate the gene expression of a broad

97 The histone chaperones CAF-1 and Rtt106p are required for heterochromatin silen

98 mplexes contain distinct histone chaperones, CAF-1 and HIRA, that we show are necessary to mediate DN

99 s to identify the chromatin assembly complex CAF-1 as a context-specific repressor of Notch signaling

100 plex NURF and the chromatin assembly complex CAF-1.

101 on-coupled chromatin assembly factor complex CAF-1.

102 ase requires the histone chaperone complexes CAF-1 (Cac2p, Msi1p and Rlf2p) and RCAF (Asf1p plus acet

103 Here, we have purified a complex containing CAF-1 and H3 and H4 from yeast cells and determined the

104 We also observed a dimeric CAF-1-H3-H4 supercomplex in which two H3-H4 heterodimers

105 The mechanisms that enable CAF-1 and other PCNA-binding proteins to function harmon

106 tides are part of the mechanism that enables CAF-1 to function behind replication forks without inter

107 budding yeast deleted for the genes encoding CAF-1 are highly sensitive to double-strand DNA-damaging

108 96 amino acids are dispensable for essential CAF-1 function in vivo.

109 in human MGMT-deficient cell-free extracts, CAF-1-dependent packaging of irreparable O(6)-mG-T mispa

110 (ASF1) plays a central role in facilitating CAF-1-mediated replication-dependent H3.1 deposition and

111 d new role for the chromatin assembly factor CAF-1 and the histone-regulating Hir proteins has been d

112 p150 subunit from chromatin assembly factor (CAF-1 p150).

113 of the Drosophila chromatin assembly factor (CAF-1).

114 a subunit of the chromatin assembly factor, CAF-1, and copurifies with the human histone deacetylase

115 cation-associated chromatin assembly factor, CAF-1, binds to and specifically maintains VEX1 compartm

116 not unreplicated, DNA is also competent for CAF-1-dependent chromatin assembly.

117 f both histone H3 and H4 are dispensable for CAF-1-mediated formation of nucleosome cores onto newly

118 y reveal interactions that are essential for CAF-1 function in budding yeast, and importantly indicat

119 r, the data illustrate a clear mechanism for CAF-1-associated H3-H4 chaperone activity in the context

120 e tetramerization, providing a new model for CAF-1-H3/H4 architecture and function during eukaryotic

121 ific acetylation sites are not necessary for CAF-1-dependent nucleosome assembly onto replicated DNA.

122 stones, possibly to maintain opportunity for CAF-1 recruitment and nucleosome assembly.

123 al reporters, overcoming the requirement for CAF-1 in transcriptional silencing.

124 Thus, these studies reveal a novel role for CAF-1 and Rtt106p in epigenetic silencing and indicate t

125 e into chromatin, consistent with a role for CAF-1 in chromatin assembly in vivo.

126 gestion demonstrated that the chromatin from CAF-1 mutant yeast has increased accessibility to these

127 ps as a byproduct of its repriming function, CAF-1's role in its recruitment could directly impact ss

128 Furthermore, CAF-1 is not required for repair of the DNA per se or fo

129 How CAF-1 ensures there is sufficient space for the assembly

130 However, how CAF-1 functions in this process is not yet well understo

131 Here, we investigate how CAF-1 influences chromatin dynamics and TF activity duri

132 However, it is unknown how CAF-1 binds and delivers H3-H4 to the DNA.

133 assembly revealed that both dCAF-1 and human CAF-1 mediate chromatin assembly preferentially with pre

134 existence of two PCNA binding sites in human CAF-1, but the defect in PCNA binding had no effect on t

135 re we report that the large subunit of human CAF-1 (p150) contains two distinct PCNA interaction pept

136 The p150 subunit of human CAF-1 contains an N-terminal domain (p150N) that is disp

137 rk, we report the crystal structure of human CAF-1 in the absence of histones and the cryo-electron m

138 DNA replication in vitro, as seen with human CAF-1.

139 s indicate that, in contrast to yeast, human CAF-1 is necessary for coupling chromatin assembly with

140 with the DNA-binding winged helix domain in CAF-1 to overcome DNA damage sensitivity and maintain si

141 ir protein recruitment to the silent loci in CAF-1 mutants, probably as a consequence of the weakened

142 The systematic rDNA loss in CAF-1 mutants leads to the decreased variability of the

143 , termed the HP1BD, which is also present in CAF-1 p150 but not SP100 or LBR.

144 n critical function of the middle subunit in CAF-1.

145 We have found that MutSalpha inhibits CAF-1- and ASF1A-H3-H4-dependent packaging of a DNA mism

146 n replication-coupled nucleosome assembly is CAF-1-dependent histone (H3-H4)2 tetramer deposition, a

147 Cells lacking CAF-1 accumulated in early and mid S-phase and replicate

148 Cells lacking CAF-1 and RCAF are hypersensitive to DNA-damaging agents

149 r proteins are mislocalized in cells lacking CAF-1 and Rtt106p.

150 cus is dramatically reduced in cells lacking CAF-1, Rtt106p, and Sir1p.

151 nous 2mu plasmid is reduced in yeast lacking CAF-1.

152 At the HMR locus, CAF-1 and Rtt106p are required for the initial recruitme

153 The MBD1-CAF-1 p150 interaction requires the methyl-CpG binding d

154 Mechanistically, CAF-1 suppression led to a more accessible chromatin str

155 functions among the DNA methyltransferases, CAF-1, and histone-modifying enzymes.

156 Unlike Asf1, monomeric CAF-1 binds to multiple H3/H4 dimers, which ultimately p

157 bserved for corresponding mutations in mouse CAF-1.

158 d in eukaryotic cells whereby the ability of CAF-1 to bind DNA is important for its association with

159 to DNA damage is dependent on the ability of CAF-1 to bind PCNA, indicating that PCNA may recruit CAF

160 p48 can bind to histone H4 in the absence of CAF-1 p150 and p60.

161 specific H3-H4 histone chaperone activity of CAF-1.

162 A and, by increasing the binding affinity of CAF-1 and Rtt106 for histone H3, H3K56Ac enhances the ab

163 sponsible for the low micromolar affinity of CAF-1 for PCNA, whereas the presence of a negative charg

164 nd H3K56Ac increases the binding affinity of CAF-1 toward H3-H4 2-fold.

165 arge subunit, Cac1 organizes the assembly of CAF-1.

166 Moreover, the association of CAF-1 with H3 methylated at lysine 79 appeared to occur

167 HIRA to H3.3-H4 and reducing association of CAF-1 with H3.1-H4.

168 tetramers and the phenotypic consequences of CAF-1-associated assembly defects are not well understoo

169 Deletion of CAF-1 p150 subunit impairs the silencing of many genes i

170 Depletion of CAF-1 from various cell lines causes replication fork ar

171 Depletion of CAF-1 in human cell lines demonstrated that CAF-1 was re

172 nd-joining pathways and that the function of CAF-1 during double-strand repair is distinct from that

173 We show that this is independent of CAF-1's nucleosome deposition function but instead may r

174 nt chromatin structure, because induction of CAF-1 after DNA damage is sufficient to restore viabilit

175 H4 tetramer and the consequent inhibition of CAF-1-mediated nucleosome formation.

176 Loss of CAF-1 leads to a delay in chromatin maturation, albeit w

177 Our results show that loss of CAF-1 leads to a heterogeneous rate of nucleosome assemb

178 The PIP motif of CAF-1 binds to the hydrophobic pocket on the front face

179 ed the crystal structure of the PIP motif of CAF-1 bound to PCNA using a new strategy to produce stoi

180 Our study highlights how the organization of CAF-1 comprising both disordered regions and folded modu

181 Tandem affinity purification of CAF-1-interacting proteins under mild conditions reveale

182 binding had no effect on the recruitment of CAF-1 to chromatin after DNA damage or to resistance to

183 /glutamic acid/arginine-rich (KER) region of CAF-1 revealed a 128- angstrom single alpha-helix (SAH)

184 ting evidence supports a substantial role of CAF-1 in cell fate maintenance, but the mechanisms by wh

185 he N terminus is dispensable for the role of CAF-1 in DNA replication- and repair-coupled chromatin a

186 , this work identifies an unexpected role of CAF-1 in regulating PrimPol recruitment and ssDNA gap ge

187 d length of the SAH drive the selectivity of CAF-1 for tetrasome-length DNA and facilitate function i

188 nd the cryo-electron microscopy structure of CAF-1 in complex with histones H3 and H4.

189 The small subunit of CAF-1 (p48) is a member of a highly conserved subfamily

190 tein Asf1, which binds the middle subunit of CAF-1 as well as to Hir proteins .

191 nds directly to p150, the largest subunit of CAF-1, and the two proteins colocalize at sites of DNA r

192 We found that the large subunit of CAF-1, Chaf1a, requires the N-terminal KER domain to ass

193 3 lysine 79, and Cac1p, the large subunit of CAF-1, exhibited a dramatic loss of telomeric silencing

194 ls lacking both Mec1 and the Cac1 subunit of CAF-1.

195 hosphorylation status of the p150 subunit of CAF-1.

196 (CAC), which contains the three subunits of CAF-1 (p150, p60, p48) and H3 and H4, and promotes DNA r

197 Notably, suppression of CAF-1 also enhanced the direct conversion of B cells int

198 lls, and overexpression of the C terminus of CAF-1 p150 prevents the targeting of MBD1 in these cells

199 the association occurs in the C terminus of CAF-1 p150.

200 lines expressing RNAi-resistant versions of CAF-1 and showed that the N-terminal 296 amino acids are

201 tion-independent histone variant H3.3 and on CAF-1 that is specific to the replication-dependent cano

202 ing upon replication stress, is dependent on CAF-1.

203 ne histone H3-H4 heterodimer is bound by one CAF-1 complex mainly through the p60 subunit and the aci

204 d a core histone chaperone (such as NAP-1 or CAF-1) are sufficient for the ATP-dependent formation of

205 Deletion of ASF1 or CAF-1 components led to global transcriptional misregula

206 p150 (p150N) that cannot interact with other CAF-1 subunits is sufficient for maintaining nucleolar c

207 teins strongly prefers the HIRA complex over CAF-1.

208 CNA unloading, causing sequestration of PCNA-CAF-1 complexes on chromatin.

209 on was reduced approximately 10-fold in RCAF/CAF-1 double mutants.

210 bind PCNA, indicating that PCNA may recruit CAF-1 to sites of double-strand DNA repair.

211 ions of PCNA residues involved in recruiting CAF-1 to the chromatin also result in defects in differe

212 f rDNA copies, and plant lines with restored CAF-1 function (segregated from a fas1xfas2 genetic back

213 ing defects in BRCA-deficient cells restores CAF-1-dependent fork stability.

214 cleosome assembly factors, including Rtt106, CAF-1, and lysine residues of H3-H4.

215 A single CAF-1 molecule binds a cross-linked (H3-H4)2 tetramer, o

216 d the function of this domain in stabilizing CAF-1 at replication forks.

217 understand the mechanism of the tri-subunit CAF-1 complex in this process, we investigated the prote

218 Consistent with this idea, we confirm that CAF-1 synchronously binds two H3-H4 dimers derived from

219 CAF-1 in human cell lines demonstrated that CAF-1 was required for efficient progression through S-p

220 ed for tetramer assembly and discovered that CAF-1 facilitates right-handed DNA wrapping of H3-H4 tet

221 We find that CAF-1 sustains lineage fidelity by controlling chromatin

222 We found that CAF-1 suppresses the activity of the MMR system in the c

223 These results highlight that CAF-1 has prominent interactions with the DNA repair mac

224 th poly(dA:dT) sequences, which implies that CAF-1 deposits histones in a manner that counteracts res

225 Together, these data indicate that CAF-1 interacts with H3 methylated at lysine 79 during t

226 These results indicate that CAF-1 mutant yeast globally under-assemble their genome

227 Our genetic analyses indicate that CAF-1 plays a role in both homologous recombination and

228 We propose that CAF-1 has an essential role in assembling chromatin duri

229 proteins under mild conditions revealed that CAF-1 was directly associated with the KU70/80 complex,

230 Our experiments have also revealed that CAF-1- and ASF1A-H3-H4-dependent deposition of the histo

231 Here, we show that CAF-1 and Rtt106p are involved in the early stages of he

232 Here, we show that CAF-1 binds recombinant H3-H4 with 10- to 20-fold higher

233 We show that CAF-1 function at replication forks is lost in BRCA-defi

234 We show that CAF-1 mutants have a drastic reduction in DNA-bound hist

235 We show that CAF-1 suppression triggers rapid differentiation of myel

236 We suggest that CAF-1 and Asf1 function redundantly to deactivate the ch

237 These results suggest that CAF-1 binds to two H3-H4 dimers in a manner that promote

238 These data suggest that CAF-1- and ASF1A-H3-H4-dependent deposition of the histo

239 Taken together, these findings suggest that CAF-1-dependent incorporation of irreparable O(6)-mG-T m

240 Although it has been suggested that CAF-1 is involved in the maintenance of heterochromatin

241 chromatin assembly activity, suggesting that CAF-1 is required for efficient S-phase progression in h

242 c loci in yeast cells deficient for both the CAF-1 and HIR histone H3/H4 deposition complexes, consis

243 of gamma-H2AX does not depend on either the CAF-1 protein, Cac1, or Asf1.

244 Instead, the CAF-1(chromatin assembly factor I) subunit Cac2 level de

245 Here we investigate the CAF-1*H3-H4 binding mode and the mechanism of nucleosome

246 een the yeast CAF-1 subunits, and mapped the CAF-1 domains responsible for H3-H4 binding.

247 that residue substitutions in yAsf1 near the CAF-1/HIRA interface also influence yAsf1's function in

248 interactions with the other subunits of the CAF-1 complex because an N-terminal fragment of p150 (p1

249 tro and altered chromatin association of the CAF-1 large subunit in vivo.

250 genome-scale assays to demonstrate that the CAF-1 and HIR complexes independently stimulate replicat

251 Here we report that the CAF-1 protein complex, an evolutionarily conserved histo

252 Here we report that the CAF-1-ASF1 pathway promotes ssDNA gap accumulation at re

253 Together, these results reveal that the CAF-1-PCNA nucleosome assembly pathway plays an importan

254 so enhances the interaction of Asf1 with the CAF-1 subunit Cac2, H3/H4 forms a tight complex with CAF

255 the protein-protein interactions within the CAF-1-H3/H4 architecture using biophysical and biochemic

256 1 subunit functions as a scaffold within the CAF-1-H3/H4 complex.

257 This CAF-1 recycling defect is caused by lagging strand gaps

258 Thus, CAF-1 and SPT6 histone chaperones maintain cell fate thr

259 defective mutants showed reduced binding to CAF-1 in vitro and altered chromatin association of the

260 g DNA and also show reduced binding of H3 to CAF-1, a histone chaperone involved in RC nucleosome ass

261 not Chk2, was phosphorylated in response to CAF-1 depletion, consistent with a DNA replication defec

262 This drives the formation of a transient CAF-1*histone*DNA intermediate containing two CAF-1 comp

263 N-terminally truncated CAF-1 p150 was deficient in proliferating cell nuclear a

264 AF-1*histone*DNA intermediate containing two CAF-1 complexes, each associated with one H3-H4 dimer.

265 When CAF-1 was lacking, Cd4 derepression was markedly enhance

266 sistance observed in HR-deficient cells when CAF-1 or ASF1A are lost correlates with suppression of s

267 sites shows marked nucleosome depletion when CAF-1 function is reduced.

268 s greater association with target sites when CAF-1 is depleted and misregulation of target gene expre

269 Here, we studied whether CAF-1 modulates the activity of the MMR system in the cy

270 However, the mechanisms by which CAF-1 mediates the deposition of (H3-H4)(2) tetramers an

271 ate maintenance, but the mechanisms by which CAF-1 restricts lineage choice remain poorly understood.

272 he VEX-complex controls VSG-exclusion, while CAF-1 sustains VEX-complex inheritance in association wi

273 ecognition factor MutSalpha, as well as with CAF-1.

274 f Asf1 enhances the interaction of Asf1 with CAF-1.

275 , modifications on H3 and H4 associated with CAF-1 are not known.

276 ctors, including the enzymes associated with CAF-1.

277 y, in part, by promoting H3 association with CAF-1 via H3 acetylation.

278 bunit Cac2, H3/H4 forms a tight complex with CAF-1 exclusive of Asf1, with an affinity weaker than As

279 H4 that copurified with CAF-1 was a mixture of isoforms acetylated at lysines 5,

280 Furthermore, Rtt106p interacts with CAF-1 physically through Cac1p.

281 the KER SAH links functional domains within CAF-1 with structural precision, acting as a DNA-binding

282 Yeast CAF-1 contains a PIP motif on its largest subunit, Cac1.

283 lts define direct structural roles for yeast CAF-1 subunits and uncover a previously unknown critical

284 terminus of Cac1 (Cac1C), a subunit of yeast CAF-1, and the function of this domain in stabilizing CA

285 We show that yeast CAF-1 binding to a H3-H4 dimer activates the Cac1 winged

286 in the direct interaction between the yeast CAF-1 subunits, and mapped the CAF-1 domains responsible