ライフサイエンスコーパス: CRISPR-Cas9 system

1 e model of Klhl31 loss of function using the CRISPR-Cas9 system.

2 ble knockout (DKO) rats were generated using CRISPR-Cas9 system.

3 a nucleosome, constrains the activity of the CRISPR-Cas9 system.

4 the naturally occurring Type II prokaryotic CRISPR-Cas9 system.

5 double-stranded DNA (dsDNA)-for example by a CRISPR-Cas9 system.

6 ficient loss-of-function screening using the CRISPR-Cas9 system.

7 and targeted gene mutation in plants via the CRISPR-Cas9 system.

8 ncy is typically much lower than that of the CRISPR-Cas9 system.

9 h III(13) alleles in NIH 3T3 cells using the CRISPR-Cas9 system.

10 een zebrafish embryos using a widely adopted CRISPR-Cas9 system.

11 ranslocations compared with the conventional CRISPR-Cas9 system.

12 different strategies to edit genes using the CRISPR-Cas9 system.

13 are known to inhibit the widely used class 2 CRISPR-Cas9 system.

14 anscriptional activation screening using the CRISPR-Cas9 system.

15 ivator-like effector (TALE) modules, and the CRISPR-Cas9 system.

16 that underlie the mechanism of action of the CRISPR-Cas9 system.

17 ations in the iPSCs were corrected using the CRISPR/Cas9 system.

18 y of a single-stranded oligonucleotide and a CRISPR/Cas9 system.

19 poson by combining it with components of the CRISPR/Cas9 system.

20 larger than 9 kb at the pH11 locus using the CRISPR/Cas9 system.

21 ting and multiplex editing capability of the CRISPR/Cas9 system.

22 with mosaic anxa4 knockout studies using the CRISPR/Cas9 system.

23 solated CSCs were depleted of PAF1 using the CRISPR/Cas9 system.

24 lants using the highly efficient multiplexed CRISPR/Cas9 system.

25 a feasible strategy for in vivo delivery of CRISPR/Cas9 system.

26 al for genome editing applications using the CRISPR/Cas9 system.

27 t facilitate selective genome editing by the CRISPR/Cas9 system.

28 ous knock-in HCT116 cells and mice using the CRISPR/Cas9 system.

29 expression in human melanoma cells using the CRISPR/Cas9 system.

30 using Adeno-associated viral vectors and the CRISPR/Cas9 system.

31 biotin labeling with the specificity of the CRISPR/Cas9 system.

32 ITPR2-knockout HepG2 cells generated by the CRISPR/Cas9 system.

33 rom NOD/SCID/IL2rg(-/-) (NSI) mice using the CRISPR/Cas9 system.

34 ies to optimize knockout efficiency with the CRISPR/Cas9 system.

35 rated a ZIP9-mutant zebrafish strain using a CRISPR/Cas9 system.

36 larly interspaced short palindromic repeats (CRISPR)-Cas9 system.

37 d divergent features among distantly related CRISPR-Cas9 systems.

38 the remarkable mechanistic diversity of the CRISPR-Cas9 systems.

39 c applications through AAV-based delivery of CRISPR/Cas9 systems.

40 ng can be achieved by combining the TAEL and CRISPR/Cas9 systems.

41 ort Palindromic Repeats/Crispr associated 9 (CRISPR/Cas9) system.

42 ression can be activated or suppressed using CRISPR--Cas9 systems.

43 order to achieve conditional control of the CRISPR/Cas9 system, a genetically encoded light-activate

44 We show that this homing CRISPR-Cas9 system acts as an expressed genetic barcode

45 However, PfmaF disruption using CRISPR/Cas9 system affected neither DHN melanin distribu

46 hylococcus aureus cells harbouring a type II CRISPR-Cas9 system after infection with the staphylococc

47 Combining with a CRISPR/Cas9 system, Agrobacterium-mediated transformatio

48 nome-wide loss-of-function screens using the CRISPR/Cas9 system allow the efficient discovery of canc

49 The CRISPR/Cas9 system allows for such targeted mutagenesis,

50 The bacterial CRISPR/Cas9 system allows sequence-specific gene editing

51 larly interspaced short palindromic repeats (CRISPR)-Cas9 system and a transposon-disrupted allele.

52 ularly interspaced short palindromic repeat (CRISPR)-Cas9 system and measured the quantities of bindi

53 ably, NRP1 knockdown with interfering RNA or CRISPR-cas9 system and blocking using anti-NRP1 antibody

54 have been drawn between the newly discovered CRISPR-Cas9 system and the RNA interference (RNAi) pathw

55 improve our mechanistic understanding of the CRISPR-Cas9 systems and may facilitate Cas9 engineering.

56 ic repeats and associated Cas9 endonuclease (CRISPR-Cas9) system and comprises a lipid nanoparticle e

57 ere corrected to the E3/E3 genotype with the CRISPR/Cas9 system and differentiated into pure cultures

58 nts in the genome editing specificity of the CRISPR/Cas9 system and enabled its activation with tempo

59 RNA of Mycoplasma mycoides, by combining the CRISPR/Cas9 system and the yeast recombination machinery

60 w, we summarize the history and mechanism of CRISPR/Cas9 systems and explore its potential applicatio

61 epeats/CRISPR-associated protein-9 nuclease (CRISPR/Cas9) system and the turquoise killifish genome,

62 ularly interspaced short palindromic repeat (CRISPR)/Cas9 system, and in vivo selection for aminoglyc

63 the zCRISPR-Cas12a system is superior to the CRISPR-Cas9 system, and our simple crRNA engineering str

64 ivator-like effector nucleases (TALENs), the CRISPR-Cas9 system, and polyamides of heterocycles as se

65 CRISPR/Cas9 systems are a versatile tool for genome edit

66 larly interspaced short palindromic repeats (CRISPR)/Cas9 system, are systematized for fast, modular

67 cent proof of principle experiments with the CRISPR-Cas9 system as a drive mechanism.

68 The adaptation of the CRISPR-Cas9 system as a genome editing technique has gen

69 These results unveil the potential of the CRISPR/Cas9 system as a new therapeutic strategy against

70 ic modification and further expansion of the CRISPR/Cas9 system as a spatiotemporally controlled gene

71 zinc finger proteins, TALEs/TALENs, and the CRISPR/Cas9 system as nucleases for genome editing, tran

72 -strand breaks (DSBs) were generated via the CRISPR/Cas9 system at centromere-specific retrotransposo

73 editing efficiency comparable to that of the CRISPR-Cas9 system but with much lower off-target effect

74 MAVERICC also leverages the CRISPR/Cas9 system but requires no markers and yields es

75 Delivery of components of the CRISPR-Cas9 system by hydrodynamic injection resulted in

76 lease degradation and rapid clearance of the CRISPR-Cas9 system by macrophages, the large size of Cas

77 Here, we demonstrate that the CRISPR-Cas9 system can be used to edit the VACV genome r

78 for future antibiotic therapies and that the CRISPR-Cas9 system can introduce rapid and efficient mod

79 infections, and we demonstrate here that the CRISPR/Cas9 system can be adapted for antiviral treatmen

80 Our study has demonstrated that the CRISPR/Cas9 system can be used for targeted inactivation

81 rium-mediated transformation combined with a CRISPR/Cas9 system can efficiently generate targeted T-D

82 Although an efficient and targeted tool, the CRISPR/Cas9 system can have some drawbacks, including of

83 Here we show that the CRISPR/Cas9 system can restrict the JCPyV life cycle in

84 Here, we show that the CRISPR/Cas9 system can specifically target and cleave co

85 systems, such as the Streptococcus pyogenes CRISPR-Cas9 system, can be adapted such that Cas9 can be

86 Our CRISPR-Cas9 system couples a site-specific double-strand

87 ir (HDR)-based gene correction that uses the CRISPR-Cas9 system delivered as a ribonucleoprotein (RNP

88 ls by combining rAAV6 donor delivery and the CRISPR/Cas9 system delivered as ribonucleoproteins (RNPs

89 fsf9 (encoding CD137L) in NOD mice using the CRISPR/Cas9 system (designated NOD.Tnfsf9 (-/-)).

90 As, including miRNAs, can be targeted by the CRISPR/Cas9 system despite their lacking an open reading

91 The CRISPR/Cas9 system develops an adaptive immune resistanc

92 04 cells with PIK3R1 depletion introduced by CRISPR/Cas9 system displayed enhanced proliferation, mig

93 as a model, our results demonstrate that the CRISPR/Cas9 system disrupts latently integrated viral ge

94 Furthermore, the inducible CRISPR/Cas9 system dramatically decreased off-target eff

95 iciency and multiplexing capabilities of the CRISPR/Cas9 system enable a variety of otherwise challen

96 The CRISPR-Cas9 system enables global screens of gene functi

97 e (NAMPT) inhibitor, we demonstrate that the CRISPR/Cas9 system enables the generation of transient h

98 e multiplex gene-editing capabilities of the CRISPR/Cas9 system facilitate the generation of a single

99 The CRISPR/Cas9 system facilitates precise DNA modifications

100 larly interspaced short palindromic repeats (CRISPR)-Cas9 system for genome editing has greatly expan

101 re importantly, we constructed an all-in-one CRISPR-Cas9 system for fast and efficient genome editing

102 s technology leverages the simplicity of the CRISPR-Cas9 system for multiplexed targeting of specific

103 The first is the CRISPR-Cas9 system for targeted gene disruption, a large

104 discuss additional applications of modified CRISPR-Cas9 systems for use in T. gondii, such as regula

105 variety K326, we developed a dual guide RNA CRISPR/Cas9 system for combinatorial gene editing of two

106 r knockout lines, we developed a viral-based CRISPR/Cas9 system for efficient gene editing in plants.

107 Recent applications of the CRISPR/Cas9 system for generating site-specific gene kno

108 ed NIR-light-triggered remote control of the CRISPR/Cas9 system for precise genome editing.

109 We explore the potential of the CRISPR/Cas9 system for the targeted capture of genomic l

110 cterized a mosquito optimized, plasmid-based CRISPR/Cas9 system for use in U4.4 (Ae. albopictus) and

111 periments, we reconstructed the evolution of CRISPR-Cas9 systems from IS200/IS605 transposons.

112 s toolbox, we characterized a set of type II CRISPR-Cas9 systems from select bacterial genera and spe

113 osion in development of applications for the CRISPR-Cas9 system, from efficient genome editing, to hi

114 The CRISPR-Cas9 system has been adapted to generate autochth

115 Furthermore, a modified version of the CRISPR-Cas9 system has been developed to recruit heterol

116 The bacterial CRISPR-Cas9 system has been repurposed for genome engine

117 The CRISPR-Cas9 system has been widely adopted as a powerful

118 CRISPR-Cas9 system has emerged as a revolutionary gene-e

119 The bacterial CRISPR-Cas9 system has emerged as an effective tool for

120 RNA-guided genome editing with the CRISPR-Cas9 system has great potential for basic and cli

121 The CRISPR-Cas9 system has increased the speed and precision

122 The CRISPR-Cas9 system has raised hopes for developing perso

123 The repurposed CRISPR-Cas9 system has recently emerged as a revolutiona

124 The CRISPR-Cas9 system has recently emerged as a versatile t

125 The CRISPR-Cas9 system has revolutionized gene editing both

126 The CRISPR-Cas9 system has revolutionized the process of mak

127 The CRISPR-Cas9 system has successfully been adapted to edit

128 Recently, the CRISPR-Cas9 system has triggered enormous interest in th

129 The CRISPR/Cas9 system has become an efficient gene editing

130 Genome-scale CRISPR/Cas9 system has been a democratized gene editing

131 The CRISPR/Cas9 system has been employed to efficiently edit

132 The CRISPR/Cas9 system has been proposed as a cure strategy

133 The CRISPR/Cas9 system has been rapidly adopted for genome e

134 Recently, the CRISPR/Cas9 system has been used for efficient multiplex

135 The CRISPR/Cas9 system has been used to generate fluorescent

136 Genome editing using the CRISPR/Cas9 system has been used to make precise heritab

137 The simplicity and precision of CRISPR/Cas9 system has brought in a new era of gene edit

138 The CRISPR/Cas9 system has emerged as an important tool in b

139 The CRISPR/Cas9 system has greatly improved our ability to e

140 The advent of the CRISPR/Cas9 system has marked a turning point in genetic

141 wise perturbation of gene function using the CRISPR/Cas9 system has potential in screening for geneti

142 The revolutionary RNA-guided endonuclease CRISPR/Cas9 system has proven to be a powerful tool for

143 In particular, the RNA-guided CRISPR/Cas9 system has recently been engineered to creat

144 CRISPR/Cas9 system has recently been repurposed to enabl

145 The CRISPR/Cas9 system has revolutionized mammalian somatic

146 Recently, the CRISPR/Cas9 system has suggested another way to selectiv

147 The CRISPR/Cas9 system has ushered in a new era of targeted

148 y interspaced short palindromic repeat/Cas9 (CRISPR/Cas9) system has emerged in recent years as a hig

149 The unique properties of the CRISPR-Cas9 system have created new opportunities for hi

150 Bacterial type II CRISPR-Cas9 systems have been widely adapted for RNA-gui

151 CRISPR-Cas9 systems have revolutionized biotechnology, c

152 nce-specific nucleases such as TALEN and the CRISPR/Cas9 system have so far been used to disrupt, cor

153 CRISPR/Cas9 systems have been used in a wide variety of

154 arly interspaced, short palindromic repeats (CRISPR)-Cas9 system, have enabled the recruitment of tra

155 Emerging technologies such as the CRISPR/Cas9 system, high-throughput mutant identificatio

156 e engineering using the bacterial RNA-guided CRISPR-Cas9 system in animals and plants is transforming

157 or HIF-2alpha knockout using the lentiviral CRISPR-Cas9 system in human established leukemic cells w

158 w Peptide-Based Nanoparticles for delivering CRISPR-Cas9 system in its RNA form applicable in both in

159 with much lower off-target effects than the CRISPR-Cas9 system in mammalian cells.

160 em is more advantageous than the widely used CRISPR-Cas9 system in terms of specificity and multiplex

161 , individually and in combination, using the CRISPR-Cas9 system in the SCN-resistant Peking and SCN-s

162 of target mutations after microinjecting the CRISPR/Cas9 system in metaphase II (MII) oocytes and zyg

163 The introduction of the CRISPR/Cas9 system in the form of Cas9/sgRNA ribonucleop

164 and demonstrate the potential of transgenic CRISPR/Cas9 systems in antiviral research in other speci

165 The use of CRISPR/Cas9 systems in genome editing has been limited b

166 The method was also applicable to the CRISPR/Cas9 system, including CRISPR/Cas9 mutant nickase

167 o overcome these shortcomings by integrating CRISPR/Cas9 system into a temperate phage genome, removi

168 uble knockout pigs using direct injection of CRISPR/Cas9 system into developing embryos.

169 ; transgenes or genome editing tools such as CRISPR/Cas9 systems) into embryos, for creating genetica

170 The CRISPR-Cas9 system is a gene editing technique that has

171 The CRISPR-Cas9 system is a powerful tool for genome editing

172 The CRISPR-Cas9 system is a powerful tool for genome editing

173 The CRISPR-Cas9 system is a powerful tool to edit eukaryotic

174 Creating hypoallergenic cats using the CRISPR-Cas9 system is a significant step forward because

175 nclude that retroviral implementation of the CRISPR-Cas9 system is an efficient system for cellular p

176 Our CRISPR-Cas9 system is an important new tool for genetic

177 The CRISPR-Cas9 system is commonly used in biomedical resear

178 Controlling the activity of the CRISPR-Cas9 system is essential to its safe adoption for

179 The CRISPR-Cas9 system is widely used to permanently delete

180 The CRISPR/Cas9 system is a bacterial defense mechanism that

181 The CRISPR/Cas9 system is a powerful genome editing tool and

182 The CRISPR/Cas9 system is a powerful method of editing genes

183 Overall, our results demonstrate that the CRISPR/Cas9 system is a powerful tool for genome manipul

184 The CRISPR/Cas9 system is a powerful tool for studying gene

185 The CRISPR/Cas9 system is a robust genome editing technology

186 The CRISPR/Cas9 system is a technology for genome engineerin

187 CRISPR/Cas9 system is an emerging gene-editing technique

188 CRISPR/cas9 system is emerging as a novel genome editing

189 in genome engineering technologies based on CRISPR/Cas9 system is enabling people to systematically

190 A unique capability of the CRISPR/Cas9 system is multiplex genome engineering by de

191 The CRISPR/Cas9 system is widely used for genome editing.

192 ndromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system is emerging as a robust biotechnolog

193 CRISPRi, an adapted CRISPR-Cas9 system, is proposed to act as a strand-speci

194 Compared to the CRISPR-Cas9 system, it recognizes T-rich PAM sequences a

195 iew provides a comprehensive overview of the CRISPR-Cas9 system, its origin, evolution, and applicati

196 The presence of the appropriate CRISPR/Cas9 system leads to an enhancement in the freque

197 ated via chromosomal translocation using the CRISPR/Cas9 system leads to focus formation in both OVCA

198 The genetic disruption of miR-277 using the CRISPR-Cas9 system led to failures in both lipid storage

199 s induced by miR-214 overexpression, and the CRISPR-Cas9 system-mediated LHX6 knockdown reversed the

200 However, transgenic CRISPR/Cas9 system-mediated viral genome targeting has n

201 ing that should be taken into account as the CRISPR-Cas9 system moves toward clinical trials.

202 Therefore, CRISPR/Cas9 systems need to be carefully designed to avo

203 addition to defense against foreign DNA, the CRISPR-Cas9 system of Francisella novicida represses exp

204 Here we use the CRISPR-Cas9 system of genome editing to overcome this li

205 f anti-CRISPRs that specifically inhibit the CRISPR-Cas9 system of Neisseria meningitidis.

206 engineering of CAR T-cells with conventional CRISPR-Cas9 systems or novel editors that can install de

207 sing homing drives, for example based on the CRISPR/Cas9 system, our approach aims at employing one o

208 , we discuss the practical advantages of the CRISPR-Cas9 system over conventional and other nuclease-

209 The REC3 clamp is conserved across type II-B CRISPR-Cas9 systems, pointing to a potential path for en

210 n of genes and chromosomes, in bacteria, the CRISPR-Cas9 system primarily targets extrachromosomal re

211 Programmable nucleases, particularly the CRISPR-Cas9 system, provide an attractive tool for genet

212 The CRISPR-Cas9 system provides a versatile toolkit for geno

213 The CRISPR-Cas9 system provides the ability to edit, repress

214 The repurposing of the bacterial CRISPR/Cas9 system provides an effective method to disru

215 ic repeats and the Cas9 RNA-guided nuclease (CRISPR/Cas9) system provides a new opportunity to create

216 Despite the abundance of bacterial CRISPR-Cas9 systems, relatively few are effective in hum

217 Although the genome-wide specificities of CRISPR-Cas9 systems remain to be fully defined, the powe

218 Anti-CRISPR proteins (Acrs) targeting CRISPR-Cas9 systems represent natural "off switches" for

219 This CRISPR/Cas9 system represents a highly effective and sca

220 CRISPR/Cas9 system requires short guide RNAs (sgRNAs) to

221 ut was achieved by short hairpin RNAs or the CRISPR/Cas9 system, respectively, whereas enzymatic inhi

222 Deletions of TSA and TSB using the CRISPR/Cas9 system result in developmental defects in fl

223 AP1 mutant males (LAP1(-/-)), prepared using CRISPR/Cas9 system, result in a reduction of reproductio

224 Furthermore, we combined our method with the CRISPR/Cas9 system, resulting in a significant increase

225 ularly interspaced short palindromic repeat (CRISPR)-Cas9 system showed that PYCR2 loss of function l

226 PAM) requirement hinders applications of the CRISPR/Cas9 system since it restricts the targetable sit

227 Here we demonstrate that CRISPR/Cas9 systems targeting the human hemoglobin beta

228 rine genomes led to the discovery of a novel CRISPR-Cas9 system, ten antimicrobial peptides, and thre

229 erference (CRISPRi) is a modification of the CRISPR/Cas9 system that directs catalytically inactive C

230 Compact CRISPR/Cas9 systems that can be packaged into an adeno-a

231 Matrix Gla floxed mouse (Mgp.floxed) by the CRISPR/Cas9 system, that subsequently allowed the genera

232 These favorable properties have made the CRISPR-Cas9 system the technology of choice for sequence

233 In the CRISPR-Cas9 system, the efficiency of genetic modificati

234 Taking advantage of the CRISPR/Cas9 system, the genome of the T4 phage was modul

235 By CRISPR/Cas9 system, the virulent genes of the newly isol

236 The advantages of the CRISPR-Cas9 system-the ease of RNA design for new target

237 acks chromosomal loci in live cells with the CRISPR-Cas9 system, then barcodes those loci by DNA sequ

238 larly interspaced short palindromic repeats (CRISPR)-Cas9 system to the mdx mouse model of DMD to rem

239 Here, we have adapted the CRISPR-Cas9 system to a well-established murine model of

240 Additionally, by using CRISPR-Cas9 system to delete ATF3, we found that ATF3 ac

241 Applications of the CRISPR-Cas9 system to edit the genome have widely expand

242 Lastly, we used the CRISPR-Cas9 system to edit the prevalent unreported gene

243 To explore this, we utilized the CRISPR-Cas9 system to generate knock-in mouse models wit

244 In this study, we exploited the power of the CRISPR-Cas9 system to identify genes affecting the tumor

245 y co-deliver the PiggyBac transposon and the CRISPR-Cas9 system to induce long-term overexpression of

246 tive in the oatp1c1 (-/-) brain, we used the CRISPR-Cas9 system to knockdown tsh in oatp1c1 (-/-) lar

247 ata demonstrate the feasibility of using the CRISPR-Cas9 system to model loss of candidate tumor supp

248 The adaptation of the CRISPR-Cas9 system to pooled library gene knockout scree

249 Using the CRISPR-Cas9 system to target six conserved amino acid po

250 Many efforts are underway to use the CRISPR-Cas9 system to therapeutically correct human gene

251 ated in vivo in mice to deliver a functional CRISPR-Cas9 system to tumoral lung cells orthotopically

252 Here we use inducible CRISPR-Cas9 systems to develop two GEMMs which aim to mo

253 results broaden the potential application of CRISPR-Cas9 systems to neurodegenerative diseases.

254 sfection was further demonstrated to deliver CRISPR-Cas9 systems to successfully modify and reprogram

255 duction in adaptive NK cells, we optimized a CRISPR/Cas9 system to ablate genes from primary human NK

256 We used the double-nicking CRISPR/Cas9 system to conduct site-specific mutagenesis

257 We used the CRISPR/Cas9 system to delete CD38 (CD38KO) in ex vivo ex

258 into double-strand DNA breaks, we adapted a CRISPR/Cas9 system to demonstrate that targeted T-DNA in

259 In this study, we utilized the CRISPR/Cas9 system to edit the major milk allergen BLG g

260 In this study, we utilized the CRISPR/Cas9 system to edit the NANOS2 gene in pig embryo

261 Here we demonstrate the use of the CRISPR/Cas9 system to eliminate targeted chromosomes.

262 emonstrate for the first time the use of the CRISPR/Cas9 system to engineer the IL-8 gene, and tested

263 hould focus on reducing mosaicism when using CRISPR/Cas9 system to facilitate direct functional analy

264 Here, we employ a multiplex CRISPR/Cas9 system to generate a complete miR-200 family

265 2 mRNA 3'UTR in spermatogenesis, we used the CRISPR/Cas9 system to generate a deletion of the orb2 3'

266 We first used the CRISPR/Cas9 system to generate cell lines with a CRISPR-

267 We herein applied the CRISPR/Cas9 system to generate mice with point mutations

268 Here we used the CRISPR/Cas9 system to generate mono- and bi-allelic null

269 We applied CRISPR/Cas9 system to generate Mstn knock-out (KO) rabbi

270 Here we adapt the CRISPR/Cas9 system to human cells for intracellular defe

271 ression, folding, and secretion, we used the CRISPR/Cas9 system to inactivate the collagen galactosyl

272 Here, we used a binary transgenic CRISPR/Cas9 system to knockout Chinmo and Kr-h1 (primary

273 ssemination, we used a doxycycline-inducible CRISPR/Cas9 system to knockout Y5R in SK-ES-1 xenografts

274 Here, we use the CRISPR/Cas9 system to restore the expression of the dyst

275 in vivo using viral-mediated delivery of the CRISPR/Cas9 system to somatic cells of adult animals.

276 tion zebrafish mutant lines by utilizing the CRISPR/Cas9 system to specifically disrupt the two alpha

277 , in order to understand the efficacy of the CRISPR/Cas9 system to target a gene within the genome of

278 Thus, it would be useful to adapt CRISPR/Cas9 systems to common mosquito cell lines.

279 e we report the utilization of the TALEN and CRISPR/Cas9 systems to induce targeted mutations and hom

280 Expression of guide RNAs in the CRISPR/Cas9 system typically requires the use of RNA pol

281 The CRISPR-Cas9 system uses guide RNAs to direct the Cas9 en

282 ctor, we constructed a T-DNA that contains a CRISPR/Cas9 system using SpCas9 and a gRNA targeting the

283 esistance to exogenous genomic material, the CRISPR-Cas9 system utilizes a short single guide RNA (sg

284 The original CRISPR-Cas9 system was developed as a tool to alter geno

285 Thus, the applicabilitiy of the CRISPR/Cas9 system was demonstrated for the first time f

286 The CRISPR/Cas9 system was recently developed as a powerful

287 oduced the known Pten KO phenotype using the CRISPR-Cas9 system we design viruses to target a gene th

288 Using the CRISPR-Cas9 system, we confirmed that pde4d1/2 and pde4d

289 Using an engineered CRISPR-Cas9 system, we show that generating DSBs at sile

290 r CENP-A K124R knockin mutant created by the CRISPR-Cas9 system, we show that the Flag-tagged or unta

291 Using the CRISPR/Cas9 system, we integrated transgenes encoding th

292 ems, and most recently the highly accessible CRISPR/Cas9 systems, we now possess an unprecedented abi

293 The CRISPRi-dCas9 and CRISPR-Cas9 systems were utilized to repress or delete s

294 The guide strands of the CRISPR/Cas9 systems were designed to have a range of mis

295 show that genomic sites could be cleaved by CRISPR/Cas9 systems when DNA sequences contain insertion

296 Our study showed how integrating CRISPR-Cas9 system with cross-species comparison can hel

297 larly Interspaced Short Palindromic Repeats (CRISPR/Cas9) system with next-generation sequencing (NGS

298 blished a new Pcdh10 KO mouse line using the CRISPR/Cas9 system, without inserting gene cassettes to

299 It is expected that a designed CRISPR-Cas9 system would able to target extrachromosomal

300 cancer cells, it is expected that a designed CRISPR-Cas9 system would be able to target extrachromoso