ライフサイエンスコーパス: CRISPR

1 CRISPR assay diagnostic results obtained nasal swab samp

2 CRISPR knockout of LIN28B-an oncofetal RNA-binding prote

3 CRISPR technology seems likely to alleviate this problem

4 CRISPR-based diagnostic approaches are proving to be use

5 CRISPR-based knockout (KO) of ATP2C1 decreases transduct

6 CRISPR-Cas gene editing and messenger RNA-based protein

7 CRISPR-Cas genome engineering has revolutionized biomedi

8 CRISPR-Cas systems are found widely in prokaryotes, wher

9 CRISPR-Cas systems provide a uniquely powerful defense b

10 CRISPR-Cas systems provide bacteria with adaptive immuni

11 CRISPR-Cas-guided base editors convert A*T to G*C, or C*

12 CRISPR-Cas9 knockout of VINR in Drosophila cells enhance

13 CRISPR-Cas9 methods have been applied to generate random

14 CRISPR-Cas9-edited zebrafish were used as an in vivo mod

15 CRISPR-mediated CD47 and HER2 dual knockouts not only in

16 CRISPR-mediated gene editing shows promise to cure genet

17 CRISPR-targeted plasmids outnumbered their bacteriophage

18 CRISPR/Cas9 genome editing has revolutionized functional

19 CRISPR/Cas9 machinery delivered as ribonucleoprotein (RN

20 CRISPR/Cas9 technologies have revolutionized our underst

21 CRISPR/Cas9-mediated abrogation of CBFA2T3 resulted in s

22 CRISPR/Cas9-mediated loss of Ena/VASP proteins reduced l

23 CRISPR/dCas9-mediated Foxp3-transcriptional activation e

24 similarities to and differences from Class 2 CRISPR-Cas systems, which use a single-protein effector,

25 A CRISPR/Cas9-generated rat model, with a 9-bp deletion wi

26 We also built a CRISPR interference system using a DNase-dead Cas12a to

27 Here, we demonstrate a CRISPR-Cas13-based strategy, PAC-MAN (prophylactic antiv

28 explore the conceptual validity, we design a CRISPR-array-mediated primer-exchange-reaction-based bio

29 We use LYTACs to develop a CRISPR interference screen that reveals the biochemical

30 n P. aeruginosa Specifically, we developed a CRISPR interference (CRISPRi) system to knock down expre

31 Here, we developed a CRISPR-based system for simultaneous quantification of m

32 model dicot Arabidopsis Here, we employed a CRISPR/Cas9-based approach to disrupt a subset of cytoki

33 t transcriptional regulation, we performed a CRISPR-based screen with an MLL2-dependent gene as a rep

34 Here we performed a CRISPR-Cas9 screen in human SV589 cells for genes requir

35 We performed a CRISPR-Cas9-knockout selection designed to identify host

36 edding I-PpoI nuclease by coupling this to a CRISPR-based gene drive inserted into a conserved sequen

37 Using a CRISPR-Cas9 gene activation approach, we showed that the

38 Using a CRISPR-mediated knockout screen, we identify SLC35B2 and

39 rated a ZIP9-mutant zebrafish strain using a CRISPR/Cas9 system.

40 Using a CRISPR/Cas9 Zebrafish her6::Venus reporter combined with

41 Using a CRISPR/Cas9-based engineering approach, we genetically d

42 ein (AcrVIA1) that inactivates the type VI-A CRISPR system of Listeria seeligeri Using genetics, bioc

43 Accordingly, AAV9-CRISPR treatment results in a reduction in the percent o

44 improves the efficiency of a subsequent AAV9/CRISPR treatment for repression of proprotein convertase

45 Additional CRISPR systems could expand the genomic target space, of

46 l combination of human association analysis, CRISPR genome editing in mice, animal behavioural analys

47 little guidance on how to design and analyze CRISPR-pooled screens.

48 discovery of numerous families of ancillary CRISPR-linked genes, often implicated in signal transduc

49 Ectopic expression in mouse cells and CRISPR/Cas9 base editing of endogenous AGS loci revealed

50 paced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes, a diverse family of proka

51 ls through CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa).

52 combinase polymerase amplification (RPA) and CRISPR-Cas12a derived fluorescent detection occurred in

53 technologies, single-cell RNA-sequencing and CRISPR-Cas9 barcode editing for elucidating developmenta

54 work, by combining mapping-by-sequencing and CRISPR/Cas9 genome editing methods, we isolated EXCESSIV

55 PGC1alpha in response to genetic (shRNA and CRISPR/Cas9) and pharmacologic (crizotinib) inhibition o

56 The differences between transposase and CRISPR-Cas integrase are largely architectural, and it a

57 ibe a listeriaphage ( LS46) encoding an anti-CRISPR protein (AcrVIA1) that inactivates the type VI-A

58 ISPR-Cas use was enhanced with an 'anti-anti-CRISPR' strategy.

59 ti-defense mechanisms including diverse anti-CRISPR proteins (Acrs) that specifically inhibit CRISPR-

60 Phages, in turn, have evolved diverse "anti-CRISPR" proteins (Acrs) to counteract acquired immunity.

61 onse to this immunity, many phages have anti-CRISPR (Acr) proteins that inhibit CRISPR-Cas targeting.

62 (2020) identify a new anti-CRISPR (Acr) that degrades cA(4), a cyclic oligo-adenyla

63 II CRISPR immunity by means of a potent anti-CRISPR ring nuclease variant AcrIII-1.

64 transcriptional repressor of the strong anti-CRISPR promoter.

65 Strikingly, type VI-A anti-CRISPRs (AcrVIAs) also significantly muffle the single-n

66 a Cas9-targeted plasmid was enhanced by anti-CRISPRs derived from Enterococcus conjugative elements,

67 ed strategy, PAC-MAN (prophylactic antiviral CRISPR in human cells), for viral inhibition that can ef

68 Bacterial and archaeal CRISPR-Cas systems provide RNA-guided immunity against g

69 Here, we describe an arrayed CRISPR screening method, Genome engineering-based Interr

70 ndogenous Mov10 promotes HCV replication, as CRISPR-Cas9-based Mov10 depletion decreases HCV replicat

71 ing conditional deletions in mice as well as CRISPR/Cas9 approaches to target CTNND1 in Xenopus, we i

72 short palindromic repeats/CRISPR-associated (CRISPR/Cas) system as a programmable, RNA-guided endonuc

73 aturely terminate transcription of bacterial CRISPR arrays, and we identify a widespread antiterminat

74 ntitermination in the evolution of bacterial CRISPR-Cas systems.

75 The bacterial CRISPR system can be used in experimental disease models

76 d using either floxed Th mice or viral-based CRISPR/Cas9.

77 -specific mutations in Arabidopsis At2OGO by CRISPR/Cas9 gene editing.

78 t in human induced pluripotent stem cells by CRISPR/Cas9gene editing.

79 Genome editing by CRISPR (clustered regularly interspaced short palindromi

80 kout alleles of the NANOS2 gene generated by CRISPR-Cas9 editing have testes that are germline ablate

81 se linked with PPRD, which were generated by CRISPR-Cas9 technology displayed low level of expression

82 We generated a cell culture model by CRISPR/Cas9-mediated deletion of CIB1 to study the funct

83 raries and single-base mutations produced by CRISPR base editors without requiring barcode expression

84 er find in human cells that loss of RAZUL by CRISPR-based gene editing leads to loss of E6AP at prote

85 rupting each predicted miRNA-binding site by CRISPR-Cas9 genome editing in C. elegans We developed a

86 te that the targeting mechanism specified by CRISPR/Cas9 forces integration into genomic regions that

87 ophisticated adaptive immune systems, called CRISPR-Cas, that provide sequence-specific protection ag

88 RISPR) together with their accompanying cas (CRISPR-associated) genes are found frequently in bacteri

89 interspaced, short palindromic repeats)-Cas (CRISPR-associated) systems as a type of adaptive immunit

90 interspaced short palindromic repeats-Cas9 (CRISPR-Cas9) and transposon vectors to disrupt Trp53 and

91 A-binding species diffusing in living cells: CRISPR-Cas9, TetR, and LacI.

92 Here, we have combined CRISPR gene editing and engineered separation-of-functio

93 se phenotypes can be rescued by compensatory CRISPR mutations that retarget mir-35 to the mutant egl-

94 ore, our data suggest that, in this context, CRISPR-Cas immune systems are maladaptive to the host, o

95 By contrast, CRISPR/Cas9-mediated poplar mutant nf-yb21 exhibited red

96 viruses, we designed a focused high-coverage CRISPR-Cas9 library targeting 332 members of a recently

97 However, current CRISPR-Cas-based nucleic acid biosensing has a lack of t

98 that the Sulfolobus solfataricus type III-D CRISPR complex generates cyclic tetra-adenylate (cA(4)),

99 Here, we develop a dCas9/CRISPR-based system that allows ectopic targeting of Ctf

100 Here, we describe CRISPR/Cas9-based editing of exon 1 of the HVT079 and HV

101 Here we describe CRISPR/dCas9-based enhancer-targeting epigenetic editing

102 TRIDE was tested using a specially developed CRISPR/Cas9 DNA damage induction system, capable of indu

103 Although P. furiosus has three distinct CRISPR-Cas interference systems (I-B, I-A and III-B), on

104 Paired with diverse CRISPR tools including our dual fluorescence CRISPRi/a c

105 introduced in the BMPR-IB gene applying Easi-CRISPR followed by BMP-4/7 stimulation for 72 h.

106 Furthermore, our highly efficient CRISPR/Cas9 gene editing in primordial germ cells repres

107 omere of two-cell mouse embryos using either CRISPR-Cas9 or base editors.

108 Bacteria and archaea employ CRISPR (clustered, regularly, interspaced, short palindr

109 Many prokaryotes employ CRISPR-Cas systems to combat invading mobile genetic ele

110 ae and Klebsiella pneumoniae, and endogenous CRISPR-Cas use was enhanced with an 'anti-anti-CRISPR' s

111 Deployment of RNA-guided DNA endonuclease CRISPR-Cas technology has led to radical advances in bio

112 Both an MS2-MCP system and an engineered CRISPR-Cas13 system were used to deliver APEX2 to the hu

113 ng HI patient skin samples and an engineered CRISPR/Cas9 ABCA12 KO cell line.

114 rapid evolution of mutant phages that escape CRISPR pressure.

115 Here we establish CRISPR/Cas9-mediated genome editing in S. rosetta by eng

116 that our new algorithm outperforms existing CRISPR/Cas9 sgRNA design tools.

117 This protocol thus expands CRISPR-based gene editing approaches beyond models of ro

118 A key aim in exploiting CRISPR-Cas is gRNA engineering to introduce additional f

119 (2020) conduct a focused CRISPR/Cas9 screen against NRF2 target and other redox r

120 ications such as the design of guide RNA for CRISPR experiments.

121 Finally, we create an online tool for CRISPR GUARD design.

122 t similar approaches can be used to forecast CRISPR/Cas9 gene editing outcomes in Xenopus tropicalis,

123 logy communities that contains only the four CRISPR-addressable landing pads.

124 Using a loss-of-function CRISPR screen in cells prestimulated with interferon bet

125 Furthermore, CRISPR-mediated knockout of FN3K in human liver cancer c

126 (DSBs) made by programmable nucleases (e.g. CRISPR-Cas9).

127 ent cells, a second, subsequent whole-genome CRISPR-Cas9 screen identified the LITAF-like protein CDI

128 d plasmids and permanently stored in genomic CRISPR arrays.

129 e-wide level, including screens that harness CRISPR/Cas9 genome editing, natural genetic variation, p

130 However, CRISPR-based homing gene drives proposed for this purpos

131 However, CRISPR-mediated gene editing revealed that PKA and AMPK

132 However, CRISPR/Cas9 edited F(0) animals too often demonstrate va

133 phages (which can become lysogenic), type I CRISPR-Cas immune systems cannot eliminate the phages fr

134 Type I CRISPR-Cas systems typically target foreign DNA for degr

135 pacer adjacent motif (PAM) in several type I CRISPR-Cas systems, but how the prespacers are processed

136 econd messenger produced during the type III CRISPR immune response.

137 monstrated that viruses can subvert type III CRISPR immunity by means of a potent anti-CRISPR ring nu

138 f this enzyme (named Crn2) exist in type III CRISPR systems but are uncharacterised.

139 Type III CRISPR systems detect viral RNA, resulting in the activa

140 Type III CRISPR-Cas prokaryotic immune systems provide anti-viral

141 Cas10 is the signature gene for type III CRISPR-Cas surveillance complexes.

142 re components of a CBASS built into type III CRISPR-Cas systems, where the CARF domain binds cyclic o

143 a technique that integrates immunostaining, CRISPR interference, RNAscope, and image analysis to val

144 Importantly, CRISPR-mediated silencing of Foxp3 transcription, but no

145 gh efficiency gene targeting applications in CRISPR and TALEN compatible systems.

146 with multiple expression-based phenotypes in CRISPR/Cas9 functional screening that uses single-cell R

147 s not a new idea, recent advances, including CRISPR-based gene editing, have made possible systematic

148 r, the application of nanomedicine including CRISPR nanoparticle, exosomes for the treatment of BC/TN

149 PR proteins (Acrs) that specifically inhibit CRISPR-Cas and therefore have enormous potential for app

150 have anti-CRISPR (Acr) proteins that inhibit CRISPR-Cas targeting.

151 By integrating CRISPR screens and single-cell RNA-sequencing profiling,

152 ewing the recent developments of integrating CRISPR sensing in miniaturized sensors for point-of-care

153 covers the technical aspects of integrating CRISPR/Cas technology in miniaturized sensors for analys

154 al Perspectives Companion Paper: Integrating CRISPR Engineering and hiPSC-Derived 2D Disease Modeling

155 explored a clinically translatable intronic CRISPR (clustered regularly interspaced short palindromi

156 catalytically impaired Campylobacter jejuni CRISPR-associated protein 9-fused adenine base editor (C

157 These results were phenocopied with a KDM3B CRISPR/Cas9 knockout.

158 p to the DNA recognition lobe in full-length CRISPR-Cas9.

159 Pooled library CRISPR/Cas9 knockout screening across hundreds of cell l

160 While targeted mutagenesis approaches like CRISPR/Cas9 now permit gene-level investigation of these

161 t and directional spacer integration in many CRISPR systems.

162 Using green fluorescent protein-FKBP-MCAK CRISPR cells we found that one deleterious hot-spot muta

163 In parallel, we generated multiple CRISPR knockouts of the fly rrp4 gene.

164 age and demonstrated its utility in multiple CRISPR/CAS9 or siRNA HTS studies.

165 itations and present methods for a multiplex CRISPR/Cas9 haploid screen in chimeric axolotls (MuCHaCh

166 This highly efficient multiplex CRISPR/Cas9_Trex2 system makes it possible to create a l

167 In this review, we discuss multiplexed CRISPR technologies and describe methods for the assembl

168 The development of new CRISPR-Cas genome editing tools continues to drive major

169 this study, we used the RNA-guided nuclease CRISPR-Cas9 (clustered regularly-interspaced short palin

170 ns in the design, execution, and analysis of CRISPR screens.

171 NA and guide RNA expand the applicability of CRISPR-associated gene editing tools in vitro and in viv

172 The clinical application of CRISPR-Cas9 gene editing has been eagerly awaited since

173 ons, broadening the research applications of CRISPR base editors.

174 Our findings inform parameter choices of CRISPR screens and provide guidance to researchers on th

175 ouse embryo fibroblasts and a combination of CRISPR-mediated gene editing and RNAi-mediated gene sile

176 s help to explain the patchy distribution of CRISPR-Cas immune systems within and between bacterial s

177 While the diversity of CRISPR alleles has been explored, the associated structu

178 As the functional diversity of CRISPR-Cas and parallel systems is further explored, RNA

179 Here we report the engineering of CRISPR Artificial Splicing Factors (CASFx) based on RNA-

180 A hallmark of CRISPR-Cas immunity systems is the CRISPR array, a genom

181 ast fivefold, highlighting the importance of CRISPR-mediated defence against plasmids.

182 With the introduction of CRISPR cleavage activity into the E-DNA sensor, a more a

183 w, we summarize the history and mechanism of CRISPR/Cas9 systems and explore its potential applicatio

184 nipulation is emerging as a powerful mode of CRISPR-based engineering.

185 developing a general thermodynamic model of CRISPR-Cas binding dynamics, our results unravel a compr

186 ion of genes, allowing multifunctionality of CRISPR.

187 The premise of CRISPR/Cas centers on the cleaving of one or both DNA st

188 In the presence of CRISPR-Cas immunity, full-length AcrIIA1 uses its two-do

189 first illustrate the molecular principle of CRISPR functioning process from sensing to actuating.

190 To realize the promise of CRISPR-Cas9-based genetics, approaches are needed to qua

191 Here, in a massive reanalysis of CRISPR tiling data using the most comprehensive feature

192 Rho to facilitate complete transcription of CRISPR arrays.

193 Sc(++) and HiFi-Sc(++) extend the use of CRISPR editing for diverse applications.

194 This study marks the first use of CRISPR/Cas9 HDR for gene integration in channel catfish

195 (2020) engineered new variants of CRISPR base editors that make precise genomic edits in r

196 challenges with, and exciting prospects of, CRISPR based biosensing developments are discussed.

197 assess the impact of cellular p53 status on CRISPR-Cas9 screen performance, we carried out parallel

198 ARCO or IL37 receptor (IL37R) by antibody or CRISPR knockout of IL37 in lung cancer cell lines repola

199 acids e.g. restriction-modification (R-M) or CRISPR-Cas systems.

200 the application of in vivo whole organismal CRISPR screening has great potential to accelerate the d

201 s other meta-analysis methods when using our CRISPR screen as validation data.

202 lows for tunable and reversible control over CRISPR-Cas9 activity.

203 screen performance, we carried out parallel CRISPR-Cas9 screens in wild-type and TP53 knockout human

204 efine potential biomarkers for TOP2 poisons, CRISPR hits were overlapped with genes whose expression

205 ur approach to facilitate image-based pooled CRISPR screens.

206 archers on the design and analysis of pooled CRISPR screens.

207 Using pooled CRISPR-Cas9 knockout screens, we showed that teratomas c

208 The present CRISPR/Cas9 gene editing dogma for single guide RNA (sgR

209 th PTPN14 degradation by HPV16 E7 and PTPN14 CRISPR knockout repress keratinocyte differentiation-rel

210 Recently, CRISPR-Cas technology has opened a new era of nucleic ac

211 g techniques, including a highly regulatable CRISPR/Cas9 strategy to induce DNA double strand breaks

212 ularly interspaced short palindromic repeat (CRISPR)-mediated gene editing and acetate supplementatio

213 larly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes, a diverse fam

214 larly interspaced short palindromic repeats (CRISPR) screens to understand endocrine drug resistance,

215 larly Interspaced Short Palindromic Repeats (CRISPR) technology holds enormous potential for the scie

216 larly interspaced short palindromic repeats (CRISPR) together with their accompanying cas (CRISPR-ass

217 larly interspaced short palindromic repeats (CRISPR)-Cas9 system and a transposon-disrupted allele.

218 larly interspaced short palindromic repeats (CRISPR).

219 ularly interspaced short palindromic repeats/CRISPR-associated (CRISPR/Cas) system as a programmable,

220 ularly-interspaced short palindromic repeats/CRISPR-associated protein 9) to correct the GBE1(102C>A)

221 carry hijacked homologs of AmrZ that repress CRISPR-Cas expression and activity.

222 ginate regulator(6), is triggered to repress CRISPR-Cas immunity upon surface association.

223 otospacer adjacent motif (PAM) requirements, CRISPR/Cas9 cannot access many genetic loci.

224 Here, we present a simple and robust CRISPR-Cas12a-based approach for combinatorial genetic s

225 ng live imaging, single cell RNA sequencing, CRISPR interference, and pharmacology.

226 In this study, CRISPR/CRISPR-associated protein 9 was used to generate

227 When coupled with cas gene systems, CRISPR-Cas subtypes are highly site and taxon specific.

228 icing Factors (CASFx) based on RNA-targeting CRISPR-Cas systems.

229 Cas9 (SpyCas9), we used both self-targeting CRISPR screening and guilt-by-association genomic search

230 Notably, we found that TARK1 CRISPR plants were resistant to Pseudomonas syringae pat

231 Collectively, we report that CRISPR-mediated repression of endogenous Myd88 can effec

232 We show that CRISPR-mediated targeting of glycolysis in T cells in mi

233 Field trials at 25 locations showed that CRISPR-waxy hybrids were agronomically superior to intro

234 The CRISPR system in bacteria and archaea provides adaptive

235 Our approach thus adapts the CRISPR/Cas9 technology for memory CD8 T cells to underta

236 Matrix Gla floxed mouse (Mgp.floxed) by the CRISPR/Cas9 system, that subsequently allowed the genera

237 the regulation of EMT genes, we employed the CRISPR/dCas9 Synergistic Activation Mediator (SAM) syste

238 llmark of CRISPR-Cas immunity systems is the CRISPR array, a genomic locus consisting of short, repea

239 Rice et al suggest that the CRISPR-associated transposase ShCAST system could lead t

240 together, these results demonstrate that the CRISPR-Cas9 generated Gaa(c.1826dupA) murine model recap

241 are captured from invaders and added to the CRISPR array during a process called 'adaptation'.

242 The structure of SAVED reveals links to the CRISPR system, which also generates cyclic nucleotides i

243 We used the CRISPR/Cas9 system to delete CD38 (CD38KO) in ex vivo ex

244 ata demonstrate the feasibility of using the CRISPR-Cas9 system to model loss of candidate tumor supp

245 fsf9 (encoding CD137L) in NOD mice using the CRISPR/Cas9 system (designated NOD.Tnfsf9 (-/-)).

246 M2 levels was rescued in cell lines with the CRISPR/Cas9-mediated knockout of 4E-BP1.

247 In a population of these CRISPR-Cas9 edited plants (n = 780) that was phenotyped

248 nd to control gene expression levels through CRISPR interference (CRISPRi) and CRISPR activation (CRI

249 Here, we performed high-throughput CRISPR screening using a ubiquitin regulator-focused sin

250 We start with a short introduction to CRISPR/Cas systems and the different effector proteins a

251 hough many cell types have been subjected to CRISPR/Cas9-mediated gene editing, there is no evidence

252 onducted the so far largest fully transgenic CRISPR screen in any metazoan organism, which further su

253 s include diverse and previously undescribed CRISPR-Cas systems, transfer RNAs (tRNAs), tRNA syntheta

254 dentify roles for Y RNAs in mammals, we used CRISPR to generate mouse embryonic stem cells lacking on

255 We used CRISPR-Cas9 to engineer human cell lines expressing POLE

256 Using CRISPR and small-molecule inhibitor screens combined wit

257 Using CRISPR-Cas9 knockout technology, we show that these two

258 Using CRISPR-Cas9, we disrupted the expression of ZFP628 in th

259 Using CRISPR-generated mouse models and biochemical assays, we

260 Using CRISPR/Cas9 editing to endogenously tag receptors with f

261 Using CRISPR/Cas9 genome editing, the enhancer cluster or part

262 Using CRISPR/Cas9-mediated gene editing, coupled with endocrin

263 study, we applied an unbiased approach using CRISPR screening to identify genes that strongly regulat

264 stem cell models and functional assays using CRISPR/Cas9 to study TNNT2 variant pathogenicity and pat

265 y dual genetic inactivation of KDM6A/B using CRISPR/Cas9.

266 Here, we have created a mouse model by using CRISPR technology to mutate a single internal translatio

267 e introduced into naive CD8(+) T cells using CRISPR-based homology-directed repair.

268 actor 1 gene (FAF1) in DLD-1 CRC cells using CRISPR/Cas9 gene editing; some cells were transfected wi

269 nctionally altering memory CD8 T cells using CRISPR/Cas9-mediated targeted gene disruption under the

270 repetitive sequences can be eliminated using CRISPR-mediated RNA targeting, yet evidence of its in vi

271 ne allele of Eprs globally (Eprs(+/-)) using CRISPR-Cas9 technology or in a Postn-Cre-dependent manne

272 Homozygous deletion of ETV4, using CRISPR/Cas9, led to greatly reduced ER binding at the ma

273 ort that tKO C2C12 myoblasts generated using CRISPR/Cas9 method differentiate despite the expected de

274 Here, using CRISPR Cas9 genome-wide mutagenesis to screen for geneti

275 Here, using CRISPR-Cas9-mediated mutations, we report that human HCT

276 bers in tobacco (Nicotiana tabacum L.) using CRISPR site-directed mutagenesis and overexpression assa

277 itically, disruption of GAL5.1 in mice using CRISPR genome editing significantly reduced GAL expressi

278 s, we generated a Prcd-KO animal model using CRISPR/Cas9.

279 The BMPR-IB gene was knocked out using CRISPR-Cas technology in granulosa cells and cultured in

280 which the MT1-MMP gene was knocked out using CRISPR/Cas9.

281 orough genomic analysis was performed, using CRISPR-Cas9 to delete MafK-int6 binding region in IRF8 e

282 ional, and posttranslational reporters using CRISPR interference (CRISPRi) with barcoded expression r

283 ression can be activated or suppressed using CRISPR--Cas9 systems.

284 Loss of IHH, another Hh ligand, by in vivo CRISPR led to more aggressive tumor growth suggesting th

285 Specifically, it investigates whether CRISPR-mediated integration of the HiBiT luminescent pep

286 Abi-/Enza-resistant LNCaP-95 cells in which CRISPR-Cas9 was used to knockout AR-FL or AR-V7 alone or

287 We conducted an in vivo genome-wide CRISPR activation screen in CTCs from breast cancer pati

288 ar ATP-the ATPome-we conducted a genome-wide CRISPR interference/activation screen integrated with an

289 umorigenesis, here we employed a genome-wide CRISPR knockout screening approach to systemically ident

290 rategy, DEADPOOL, we performed a genome-wide CRISPR screen and identified IPO11 as a required factor

291 Here, we perform a genome-wide CRISPR screen using an endogenous cholesterol reporter a

292 lymphocytes (CTLs), we performed genome-wide CRISPR screens across a panel of genetically diverse mou

293 Genome-wide CRISPR screens enable systematic interrogation of gene f

294 cer-spheroid model and performed genome-wide CRISPR screens in 2D monolayers and 3D lung-cancer spher

295 Here we performed genome-wide CRISPR screens in Vero-E6 cells with SARS-CoV-2, Middle

296 encoding a catalytically inactive Adar with CRISPR/Cas9.

297 m and The Cancer Genome Atlas databases with CRISPR/Cas9-mediated depletion of the zinc finger E-box

298 e expression changes in induced neurons with CRISPR (clustered regularly interspaced short palindromi

299 tent stem cell (hiPSC)-based technology with CRISPR-based genome engineering facilitates precise isog

300 In recent years, CRISPR-associated (Cas) nucleases have revolutionized th