ライフサイエンスコーパス: Cys residue

1 was responsible for 58% loss of free thiols (Cys residues).

2 hrough a C-terminal, N-terminal, or a middle Cys residue.

3 argeted mutant encoding an altered catalytic Cys residue.

4 owing its mono-ubiquitination at a conserved Cys residue.

5 methylation of the terminal Gtgamma subunit Cys residue.

6 intracellular organic hydroperoxides through Cys residue.

7 in chains to a protein substrate through its Cys residue.

8 and subsequent exposure of previously buried Cys residues.

9 Fe-2S] cluster coordinated by four conserved Cys residues.

10 namic disulfide formation involving multiple Cys residues.

11 N-degron pathway is oxidation of N-terminal Cys residues.

12 prediction and classification of functional Cys residues.

13 ere observed only in the vicinity of the two Cys residues.

14 what attained by acrylamides when targeting Cys residues.

15 extended backbone conformation at one of the Cys residues.

16 iation does not involve conserved N-terminal Cys residues.

17 ed the Nt-acetylated Ala, Val, Ser, Thr, and Cys residues.

18 into peptides and proteins by alkylation of Cys residues.

19 -terminal tail of Oxa1L does not contain any Cys residues.

20 during evolution and by clustering of other Cys residues.

21 m, determine the solvent exposure of the two Cys residues.

22 anes is due to palmitoylation of one or more Cys residues.

23 u-217 and Lys-224, in the second mutant with Cys residues.

24 yanin (KLH) carrier protein through backbone Cys residues.

25 third Cys loop between the fourth and fifth Cys residues.

26 s, in a mutant of DGK lacking the two native Cys residues.

27 e specific for substrates bearing N-terminal Cys residues.

28 s, in one of these domains, or without these Cys residues.

29 ophore binding depend on the location of the Cys residues.

30 subunit by individually replacing them with Cys residues.

31 fhydryl group present in Cys, for the native Cys residues.

32 are formed between adjacent Nav1.2 (912/918)Cys residues.

33 of Trx proteins at a non-catalytic cysteine (Cys) residue.

34 serine (Ser), threonine (Thr), and cysteine (Cys) residues.

35 loop (ECL) containing 16 conserved cysteine (Cys) residues.

36 Cys residues using OxICAT revealed that 381 Cys residues (33.6%) showed >10% increased oxidations un

37 ted in a higher basal oxidation level of 338 Cys residues (41.1%).

38 nd that, after addition of a single-terminal Cys residue, a CdtB homologue from cytolethal distending

39 nd iRFP713 proteins and their mutants having Cys residues able to bind BV either in both PAS (Cys15)

40 ent with iron ligation of the "noncanonical" Cys residue across subunit interfaces of the Td SOR homo

41 ues and contain ten evolutionarily conserved Cys residues across a wide variety of species.

42 e dimer formation, suggesting that these two Cys residues act as vicinal thiols, consistent with C119

43 that a mutation, C39A, of a highly conserved Cys residue among NCS proteins, increases the apparent c

44 nase (normally 27.2 A distant) with a single Cys residue and introduce new termini at a surface loop,

45 l death, which required intact extracellular Cys residues and a conserved kinase active site.

46 PLP1s is active, and the predicted catalytic Cys residues and cleavage sites for each PLP1 were confi

47 The functionality of the predicted catalytic Cys residues and cleavage sites was tested by analysis o

48 Titration of reduced Cys residues and comparative mass spectrometry on AtSBP1

49 onsistent with cluster coordination by three Cys residues and one oxygen-containing residue, and anal

50 Other GAF domain proteins substitute the Cys residues and others to specifically bind cyclic nucl

51 stored through the introduction of a pair of Cys residues and the subsequent formation of a disulfide

52 econdary structure, accessibility of Trp and Cys residues, and aggregation), in plasma membranes as w

53 disulfide bond arrangement of the conserved Cys residues, and it also extends the structural knowled

54 ylation or disulfide bridges involving these Cys residues appear to be required for ER exit of APP.

55 Since the ten Cys residues are highly conserved in Meteorin and Cometi

56 The conserved RecB motif and four Cys residues are important for DNA binding and cleavage

57 or the functional defects when the conserved Cys residues are mutated.

58 indicating that the microenvironments of the Cys residues are mutually interdependent.

59 Further, these Cys residues are not palmitoylated in Rpe65 by mass spec

60 However, in the control cells, these Cys residues are occupied and produced less labeling wit

61 Eight additional Cys residues are oxidized at high versus low pO2 only wh

62 eptide, HD5[Ser(hexa)], where all six native Cys residues are replaced by Ser residues, was also eval

63 l as other lecithin:acyltransferases wherein Cys residues are required for catalysis.

64 ytochrome c and the identity of their active Cys residues are unknown.

65 fold and a catalytic triad (or dyad) with a Cys residue as a nucleophile.

66 a long loop between helices I and II, and a Cys residue as a quencher for the acceptor.

67 d in detail the role of the highly conserved Cys residue as well as the geometry and stereochemistry

68 al Lys and Arg residues with Ala and added a Cys residue at either position 289 or 275 to affix a flu

69 n analyzing a K14 variant harboring a single Cys residue at position 367.

70 s of this protein with a specifically placed Cys residue at position 4, 39, 67, or 94 of Oxa1L-CTT ha

71 ontaining peptides, but the requirement of a Cys residue at the ligation junction can limit the utili

72 -specific drug conjugation at the engineered Cys residue at the position 239 of HC does not impact th

73 rast, mutants with Cys in the PAS only or no Cys residues at all exhibit red-shifted emission with sh

74 The introduction of Cys residues at homologous sites within either the beta

75 s S-palmitoylated on two adjacent C-terminal Cys residues at its cytoplasmic surface.

76 residue polypeptide chain of sfAFP contains Cys residues at positions 1, 13, 28, and 43 and was prep

77 Likewise, the covalent modification of Cys residues at selected positions in the beta-ball-thum

78 Disulfide bonds formed between Cys residues at six positions in Ste2.

79 he formation of a disulfide bond between the Cys residues at the apocytochrome c heme-binding site (C

80 ) ions, which do not coordinate or influence Cys residues at the designed metal sites but are essenti

81 d GPR109b identified a common structure (six Cys residues at the extracellular domains that potential

82 al for GPR81 function, and the conserved six Cys residues at the extracellular regions are necessary

83 hat BACE1 undergoes S-palmitoylation at four Cys residues at the junction of transmembrane and cytoso

84 The cysteine (Cys) residue at position 312 in the third transmembrane

85 ovided by IAM is more suitable to label free Cys residues, avoiding nonspecific metal dissociation.

86 an be derived from maurocalcine by replacing Cys residues by isosteric 2-aminobutyric acid residues a

87 Oxidative modification of Cys residues by NO results in S-nitrosylation, a ubiquit

88 ed by further lipid modification of adjacent Cys residues by one (N-ras) or two (H-ras) palmitoyls.

89 Substitution of both D3 Cys residues by Sec in the UAUA variant does not elimina

90 As predicted by this model, when Cys residues C141, C144, and C227 are mutated to alanine

91 of the heavy chain pairing with the terminal Cys residue (C214) in the light chain.

92 Ala substitutions at an additional conserved Cys residue (C291 in AtsB and C276 in anSMEcpe) afford p

93 ts overoxidation and inactivation, whereas a Cys residue can be permanently overoxidized to the sulfi

94 lation sites, Asp208 located between the two Cys residues, cannot undergo proper glycosylation, which

95 Substitution of Tyr81 with a Cys residue, characteristic of established NIP boric aci

96 These three conserved Cys residues cluster with a previously unrecognized cons

97 s of each, and that the peptides contain six Cys residues, consistent with identities as alpha-defens

98 a Cu(4)S(6) species, and suggesting that D2 Cys residues contribute to the cluster.

99 d Hydrogenovibrio marinus, two supernumerary Cys residues coordinate the proximal [FeS] cluster in Ec

100 ed atomic force microscope tip to engineered Cys residues could be described by the worm-like chain m

101 the very N-terminus of Rrp44p contains three Cys residues (CR3 motif) that are conserved in many euka

102 dditional loop KSGKPLH, invariable cysteine (Cys) residues Cys-40 and Cys-168, and the conserved glut

103 ke proteins, Cr-TRP16 contains an additional Cys residue (Cys-15, at the N terminus), through which i

104 investigate its interaction with a conserved Cys residue (Cys-57) in SSP.

105 (Arg 86, Arg 91, Lys 100) and the anchoring Cys residues (Cys 85, Cys 101) within this motif were re

106 stant of the oxidation of PDI's redox-active Cys residues (Cys(53) and Cys(397)) by hydrogen peroxide

107 agment (residues 98-118), which contains two Cys residues (Cys-106 and Cys-112), was singly palmityla

108 mutations of either the last three alpha ECL Cys residues (Cys-14, -15, and -16) or Cys-7 within both

109 tion of ERp44C29S with SERT, which has three Cys residues (Cys-200, Cys-209, and Cys-109) on the seco

110 ever, VKOR contains evolutionarily conserved Cys residues (Cys-43 and Cys-51) that reside in a loop o

111 It uses a key Cys residue, Cys-13, to sense oxidative stress and to co

112 A conserved Cys residue, Cys-24, plays the major role of oxidative s

113 (i) Among its four Cys residues, Cys(52), Cys(83), and Cys(173) can be glut

114 al pocket residues (His64 and Val68) and two Cys residues (Cys55 and Cys120).

115 Mutation of two membrane-proximal Cys residues (Cys88/91) suppresses palmitoylation, and t

116 All four Cys residues (Cys9, 37, 40, and 46) in the N-terminal re

117 donor and acceptor probes at two engineered Cys residues designed to detect relay helix bending.

118 Replacing Ser239 with a Cys residue does not alter the exchange kinetics of the

119 Both proteins contain five conserved Cys residues, each required for turnover.

120 coli RNA polymerase based on the ability of Cys residues engineered into the TL and surrounding regi

121 of sources has identified a single conserved Cys residue essential for catalytic activity.

122 r in SepCysS by 3-fold, suggesting that this Cys residue facilitates the generation of the persulfide

123 oss-linking between selectively incorporated Cys residues finds two pairs of positions that provide f

124 ally, we demonstrated that a palmitoylatable Cys residue flanking the MYR site is crucial to localize

125 alpha-helical domain containing 14 conserved Cys residues followed by a glycosaminoglycan attachment

126 substitution of an evolutionarily conserved Cys residue for an Arg in the gene product.

127 CYP2B6 downregulation, and selected Tyr and Cys residues for mutation based on predicted solvent acc

128 Here, we show that the conserved Cys residues form a disulfide bond that inactivates the

129 MS was used to identify the specific Tyr and Cys residues forming radicals in the myoglobin system.

130 s: C8 evasins share a conserved set of eight Cys residues (four disulfide bonds), whereas C6 evasins

131 an intersubunit disulfide bond with a second Cys residue from the other subunit of the protein dimer.

132 luster is coordinated by the three invariant Cys residues from one monomer and, unexpectedly, Asp8 fr

133 ss the RsrR monomer-monomer interface by two Cys residues from one subunit and His and Glu residues f

134 cause of the large increases in exposure for Cys residues from T13 to T51, we conclude that colicin B

135 MTSEA-biotin only interacts with the free Cys residues from the external phase of the plasma membr

136 We hypothesized that the deviation of Cys residues from the properties of a free amino acid is

137 exchange reactions between selected pairs of Cys residues from these proteins.

138 vide an overview of approaches used to study Cys residues, from methods for investigation of their ba

139 riants in which each of the two coordinating Cys residues has been individually mutated to Ala, using

140 f factors, palmitoylation of its cytoplasmic Cys residues has not been previously described.

141 However, the status of the Cys residues has remained unknown.

142 These Cys residues have been derivatized with a fluorescent pr

143 that are capable of identifying nitrosylated Cys residues have been developed.

144 The NIR FPs with both Cys residues have the narrowest blue-shifted spectra and

145 proteins that, although coordinated by four Cys residues, have different cluster environments.

146 conspicuously similar to that of a critical Cys residue in BtrN, another radical SAM dehydrogenase.

147 f S105 alleles encoding holins with a single Cys residue in different positions was developed and cha

148 ant located six residues after the conserved Cys residue in extracellular loop 2b (ECL2b) associated

149 unique chemistry of the invariant catalytic Cys residue in labeling the active site with biotinylate

150 ase of Escherichia coli (LacY) with a single-Cys residue in place of A122 (helix IV) transports galac

151 al gB proteins, the HCMV fusion factor has a Cys residue in the C-terminal region that is palmitoylat

152 compound covalently bound to a nucleophilic Cys residue in the catalytic site of the corresponding p

153 erredoxin-like fold that binds Cu(I) via two Cys residues in a M(10)X(11)C(12)X(13)X(14)C(15) motif l

154 Disulfide bonds between Cys residues in adjacent strands of parallel beta-sheets

155 Mutagenesis of all six Cys residues in ATIII to Ala resulted in its efficient s

156 d that only the first of the two active-site Cys residues in ATTRX5 is required for the response to v

157 By introducing site-directed Cys residues in bacterial iron transporters and modifyin

158 BphP1-FP, iRFP670 and iRFP682 have Cys residues in both PAS and GAF domains, rather than in

159 These data suggest that Cys residues in D2 of hCCS are involved in the formation

160 We genetically introduced pairs of Cys residues in different regions of the FepA tertiary s

161 activity, reverts the oxidation of invariant Cys residues in FNIP1 and allows CUL2(FEM1B) to recogniz

162 found that mutation of the conserved His and Cys residues in HCCHp had little effect on secondary str

163 Mutation of one or both conserved Cys residues in HCCHp led to a decrease in Cys ligation,

164 eneration of filopodia is independent of the Cys residues in its redox active site, but dependent upo

165 ways via post-translational modifications of Cys residues in key regulatory proteins.

166 e role of a disulfide bond formed by vicinal Cys residues in maintaining calcium-bound TG2 in an inac

167 We present here that two Cys residues in MexR are redox-active.

168 Our analysis revealed that the ten Cys residues in murine Meteorin form five disulfide bond

169 We investigated the functional role of these Cys residues in Na(+) self-inhibition, an allosteric inh

170 igh-throughput and unbiased discovery of SNO-Cys residues in proteins from complex biological samples

171 d Tyr should undergo a similar reaction with Cys residues in proteins to give intramolecular or inter

172 This protocol describes the modification of Cys residues in proteins using glycomethanethiosulfonate

173 are other yet to be identified palmitylated Cys residues in RPE65.

174 y, we showed that all three highly conserved Cys residues in SepCysS (Cys(64), Cys(67), and Cys(272)

175 The CT-25 peptide contains the only two Cys residues in TE juxtaposed to a cluster of positively

176 N termini of 6K and TF, the four additional Cys residues in TF's unique C terminus, or all nine Cys

177 idues in TF's unique C terminus, or all nine Cys residues in TF.

178 rgely unaffected by modification of the same Cys residues in the absence of cross-link formation.

179 conclude that approximately half of the ECL Cys residues in the alpha and gamma ENaC subunits are re

180 previous work revealed that two cytoplasmic Cys residues in the beta subunit, betaCys-43 and betaCys

181 showed that ONOO(-) treatment modified both Cys residues in the CT-25 peptide to sulfonic acid deriv

182 n to tightly bind CO (K(d) = 50 nm) with the Cys residues in the CXXCH motif regulating affinity of t

183 ylated, we now show that the two cytoplasmic Cys residues in the gamma subunit are palmitoylated.

184 We modularly mutated the five Cys residues in the identical N termini of 6K and TF, th

185 structures and intersubunit cross-linking of Cys residues in the membrane.

186 short domain structure with an odd number of Cys residues in the metalloprotease domain.

187 This showed that all four conserved Cys residues in the N-terminal region are required for i

188 matic site-directed mutagenesis study of the Cys residues in the stem region shows that Cys(532) is i

189 ase activities in vitro, are nitrosylated in Cys residues in vivo, and scavenge NO in the stomatal ce

190 s, which are covalently bound to a cysteine (Cys) residue in the chromophore-binding domain (CBD, com

191 dynamic post-translational modifications of Cys residues, in particular S-nitrosylation and S-oxidat

192 varies with the presence or absence of other Cys residues, indicating that the microenvironments of t

193 Introduction of Cys residues into the region between residues 28 and 34

194 s are extended to TM1 and TM2 of MotA, using Cys residues introduced in several positions in the segm

195 We observe that the catalytic Cys residue is covalently linked to the small-molecule i

196 Acylation of the KS active site Cys residue is followed by transfer to malonyl-ACP to yi

197 The solvent accessibility of the Cys residues is also determined by blockage of [14C]NEM

198 , although the reversible trapping of MGO by Cys residues is initially kinetically favourable.

199 x control, acting through reactive cysteine (Cys) residues, is among the major mechanisms of redox re

200 sidue oxidizes more rapidly than a cysteine (Cys) residue, it has been previously thought that Sec-co

201 lity electron density map showing a modified Cys residue, likely connected to a long chain acyl group

202 Two conserved Cys residues located in the active site of PI4KIIIalpha

203 logs found in angiosperms contain two unique Cys residues located in the lumen.

204 eted by multiple Ox-PTM suggesting that this Cys residue may act as a redox sensor modulating ATP syn

205 In viruses with the N-terminal Cys residues mutated, TF is much less efficiently locali

206 between the catalytic Cys and one of the two Cys residues nearby, which is not observed in previously

207 l acidic residues, as well as the C-terminal Cys residue of the TP53INP1 LIR peptide, are required fo

208 -linkers covalently cross-link the catalytic Cys residue of the yeast HECT E3 ubiquitin ligase Rsp5 w

209 We show that six Cys residues of a precursor peptide are first cyclodehyd

210 csA via formation of disulfide bonds between Cys residues of adjacent subunits.

211 he active site of IscS and deliver it to the Cys residues of IscU, formed a disulfide bridge with Fdx

212 y S-nitros(yl)ation whereas the CXXC-derived Cys residues of MIF remained unaffected.

213 ite preferentially oxidizes the redox-active Cys residues of PDI to the corresponding sulfenic acids,

214 Here we show that the active site Cys residues of Prx2 form stable mixed disulfides with g

215 se basic residues are found between the loop Cys residues of several lentiviral fusion proteins, the

216 ified zinc ion and its coordination by three Cys residues of the IDC region of eukaryotic MutY organi

217 The two Cys residues of the motif are essential for the function

218 nd that reproducible saturation of available Cys residues of the proteins used as labeling standards

219 Here we report that the conserved Cys residues of Tim22 form an intramolecular disulfide b

220 and in vitro while substitution of all three Cys residues of YodB affects induction of azoR1 transcri

221 so derivatives can also react with cysteine (Cys) residues of glutathione or proteins to form, respec

222 In this study we mutated the seven cysteine (Cys) residues of human PCFT to serine, creating Cys-less

223 Cysteine (Cys) residues often play critical roles in proteins; how

224 prokaryotic THI4s that lack the active-site Cys residue on which suicide activity depends, and (ii)

225 (-1)s(-1) consistent with a key role for the Cys residues on MPI as targets for haem protein-mediated

226 e natural collagen triple helices, cysteine (Cys) residues on neighboring strands are linked by disul

227 acid, disodium salt (IASD), to modify these Cys residues, one face of transmembrane domain 1 (TMD1)

228 Removing either the Cys residue or the two His residues lowers the Cu-peptid

229 in activity induced by cross-linkage of the Cys residue pairs was due to a decrease in the influxVma

230 ce of a terminal-free thiol group, such as a Cys residue, primarily due to better cellular uptake.

231 However, substitution of Ser596 with a Cys residue produced an active recombinant enzyme with c

232 helix 3, His264 (TM6), and Met292 (TM7)--to Cys residues produced definitive indications of proximit

233 Mutation of one of these Cys residues reduced the phenylarsine oxide sensitivity

234 the complex formed with the mutant with both Cys residues replaced was nearly identical with that of

235 king this disulfide, with one or both of the Cys residues replaced with Ser, were examined, and X-ray

236 a disulfide bond formed with an active-site Cys residue required for activity.

237 activity, whereas mutations of the conserved Cys residues resulted in a loss of the iron-sulfur clust

238 in Prx3, residues adjacent to the resolving Cys residue, resulted in a Prx2-like protein with increa

239 involving initial methylation of a conserved Cys residue (RlmN Cys(355)) by SAM.

240 O2 (1% O2), but their identity among the 100 Cys residues/RyR1 monomer is unknown.

241 n is typically restricted to one or very few Cys residue(s) in target proteins.

242 The chemical reactivities of engineered Cys residues scanned throughout the aspartate receptor H

243 n, pyrene maleimide was attached to pairs of Cys residues separated by ~5 A increments on helix 2 of

244 Disulfide cross-linking based on ectopic Cys residues showed that the contacts between Gag protei

245 Cys residues subject to pO2-coupled oxidation are distri

246 overage of RyR1 Cys residues) to identify 13 Cys residues subject to pO2-coupled S-oxidation in SR ve

247 Disulfide bonds also formed with Cys residues substituted for five different residues clu

248 of Ste2, a set of 73 different mutants with Cys residues substituted near the extracellular ends of

249 ohrR gene modified for N-terminal Cysteine (Cys) residue, suggesting that OhrR senses intracellular

250 The alpha6-helix ends in 2 Cys residues that are linked by an unusual vicinal disul

251 formation of at most a few final pairings of Cys residues that may be separated by significant interv

252 ons in a previously unseen manner via mainly Cys residues that point into the core of the bundle.

253 etrameric proteins possess a large number of Cys residues that point into the cores of their four-hel

254 agnetic nitroxide spin label was attached to Cys residues that were placed into the protein at positi

255 investigated the arrangement of the His and Cys residues, the role of the spacing between them, and

256 In a cyclization process, Cys residues then attack the dehydrated residues to gene

257 In proteins with more than one pair of Cys residues, there is the potential for formation of no

258 rgI engineered with an accessible N-terminal Cys residue to 20kDa PEG-maleimide (Co-hArgI-C(PEG-20K))

259 Mutation of the palmitoylated Cys residue to Ala or inhibition of protein palmitoylati

260 partners and by inserting an extra, unpaired Cys residue to create an opportunity for intermolecular

261 through the hyperoxidation of an active site Cys residue to Cys sulphinic acid.

262 They use a conserved Cys residue to reduce peroxide substrates.

263 substrate influx as was accessibility of the Cys residues to biotinylation.

264 nitrosylation, the oxidative modification of Cys residues to form S-nitrosothiols (SNOs).

265 ively, followed by the conjugate addition of Cys residues to the Dha and Dhb residues to generate the

266 by examining the accessibility of introduced Cys residues to the sulfhydryl reagent MTSET.

267 Phase-I conversion of six Cys residues to thiazoles (TclIJN) is followed by C-term

268 spectrometry (yielding 93% coverage of RyR1 Cys residues) to identify 13 Cys residues subject to pO2

269 g di- and trisulfide cross-links between two Cys residues, to derepress the mexAB-oprM operon.

270 nce the widespread occurrence of the Trp/Cys-Cys residues triads in proteins make our procedure very

271 olecular disulfide bonds involving all three Cys residues, two in Rz and one in Rz1.

272 oxidize and form disulfide bonds with other Cys residues under oxidizing conditions, thus potentiall

273 the viral surface, covalent labeling of the Cys residues using a Cys-reactive label that masks epito

274 Quantification of the redox state of 1098 Cys residues using OxICAT revealed that 381 Cys residues

275 ation inhibitory factor contains 3 cysteine (Cys) residues; using recombinant wtMIF and site-specific

276 respectively, that are attached to specific Cys residues via thioether linkages.

277 In addition, clustering of Cys with another Cys residue was associated with high conservation, where

278 to process EbpB and its predicted catalytic Cys residue was required for efficient cell wall anchori

279 A conserved Cys residue was shown to be necessary for redox regulati

280 proteins encoded by A. oris possess multiple Cys residues, we propose that MdbA and VKOR constitute a

281 Pairs of Cys residues were assessed for spontaneous formation of

282 sonance assignments for the isotope-enriched Cys residues were determined with 2D versions of standar

283 ellular region, two CXC motifs and two other Cys residues were found to be involved in disulfide brid

284 n alpha and gamma ECLs and selected beta ECL Cys residues were individually mutated to alanine or ser

285 Cys residues were introduced at 12 positions in hSERT to

286 (6.9 +/- 0.2) x 10(4) m(-1) s(-1)), and both Cys residues were kinetically indistinguishable.

287 pO2-sensitive Cys residues were largely non-overlapping with those ide

288 S-glutathionylated nor sulfenamide-modified Cys residues were observed.

289 Linear analogs of TP-I where the four Cys residues were replaced by aromatic and aliphatic res

290 belling, indicating either that the relevant Cys residues were sequestered by an unknown protein or t

291 complexes in which all endogenous cysteine (Cys) residues were eliminated by site-directed mutagenes

292 ethyltransferases (DNMTs) through the active Cys residue, which provides a new tool to covalently tra

293 y S-oxidation or S-nitrosylation of separate Cys residues, which are allosterically linked.

294 e, providing an alternative way to transform Cys residues, which were artificially inserted into a pe

295 ion poorly for epitopes containing cysteine (Cys) residues, which can oxidize and form disulfide bond

296 Covalent modification of the Cys residues with a structurally diverse set of hydropho

297 Moreover, mutations of a single Cys residue within the TMD of Cosmc prevent formation of

298 All 16 Cys residues within alpha and gamma ECLs and selected be

299 P79/150 required its depalmitoylation on two Cys residues within the N-terminal targeting domain.

300 sidues 81 to 155, and to our surprise, the 5 Cys residues within UL16(1-155) are not required, even t