ライフサイエンスコーパス: DAPI

1 DAPI (4',6'-diamidino-2-phenylindole) staining of the ar

2 DAPI (4,6-diamidino-2-phenylindole) inhibited the assemb

3 DAPI also induced the directional movement of the nucleo

4 DAPI and immunofluorescence staining showed a single nuc

5 DAPI displacement is dependent on both RecA protein and

6 DAPI lifetime variations across interphase nuclei showed

7 DAPI lifetime variations in metaphase chromosome spreads

8 DAPI protected only the AT bp in the binding sites, whil

9 DAPI released in situ in cell culture media was incorpor

10 DAPI staining of the nucleoid demonstrated that both ori

11 DAPI staining revealed a mean cell adhesion index of 1.6

12 DAPI uptake by HeLa cells expressing mCx30.2 was >10-fol

13 DAPI was restained on a given tissue slide producing mul

14 DAPI-labeled neurons at different levels of the retina w

15 DAPI/nuclei signals were analysed with fluorescence micr

16 he impact of three DNA-binding dyes (YOYO-1, DAPI, and DRAQ5) in a concentration-dependent manner.

17 The multicyclic dyes Hoechst 33258, DAPI, and berenil bind to TAR RNA in a similar manner an

18 he flow rate of the sample introduced with a DAPI can be much higher than that allowed with a convent

19 A laboratory-scale mass spectrometer with a DAPI-RIT (rectilinear ion trap)-DAPI configuration has b

20 a preliminary model for the enhanced action DAPI.

21 In addition, DAPI was more effective than distamycin and Hoechst 3325

22 ein synthesis), segmentation of nuclei after DAPI staining (apoptosis), and colony formation in 0.2%

23 Standard groove binding agents (DAPI, distamycin, and netropsin) showed a strong prefere

24 Also, DAPI (4',6'-diamidino-2-phenylindole) staining revealed

25 Whereas for YOYO-1 and DAPI the binding mechanisms could be assigned to bis-int

26 and M2 macrophage subtypes, alpha-actin, and DAPI was performed.

27 c cell death was detected by TUNEL assay and DAPI nuclear staining.

28 d apoptosis was evaluated by TUNEL assay and DAPI staining using digital imaging.

29 ibility was assessed using the MTT assay and DAPI staining, while in vivo biocompatibility was evalua

30 guished: DAPI-positive chromatin bridges and DAPI-negative ultrafine DNA bridges (UFBs).

31 low, Alexa Fluor(350), ethidium bromide, and DAPI, which have valences of -2, -1, +1, and +2, respect

32 unofluorescent staining for TREM2, CD68, and DAPI in 95 early-stage breast cancer samples from patien

33 n (AE1/AE3), PD-L1, CD4, CD8, CD3, CD68, and DAPI, and Panel 2 included pancytokeratin, PD-1, CD45RO,

34 ng ATP to be released via these conduits and DAPI uptake directly from the channel mouth to the cytop

35 The fluorescent dyes DiI and DAPI were used to stain the coronary vasculature and car

36 PI)-sensitive foci that are bound to DNA and DAPI-insensitive foci that are DNA-less aggregates/stora

37 cleosomal fragmentation of cellular DNA, and DAPI (4',6-diamidino-2-phenylindole) staining revealed c

38 studies show that MepR binds to ethidium and DAPI with comparable affinities (K(d) = 2.6 and 4.5 micr

39 , PD-1, CD45RO, granzyme B, CD57, FOXP3, and DAPI.

40 tro by alkaline comet, DNA fragmentation and DAPI staining assays.

41 families occur in large heterochromatic and DAPI positive blocks.

42 ange, ethidium bromide, propidium iodide and DAPI staining demonstrated that cell death occurred part

43 scence microscopy after propidium iodide and DAPI staining.

44 mo-2'-deoxyuridine (BrdU) pulse-labeling and DAPI (4',6-diamidino-2-phenylindole) staining, which pre

45 ntinuous variables, replicative labeling and DAPI staining.

46 , OX40, CD27, TIM3, CD3, a tumor marker, and DAPI.

47 e two duplexes, in contrast to netropsin and DAPI, which bind with similar affinities to the two dupl

48 of PI3K signaling was examined by TUNEL and DAPI staining.

49 ng with propidium iodide, anti-Annexin V and DAPI.

50 sion of PcsB with fluorescent vancomycin and DAPI to determine patterns of cell wall synthesis and nu

51 The MTT assay, DAPI staining, and FE-SEM images confirmed the successfu

52 and proliferation of AMSCs using MTT assay, DAPI staining, and FE-SEM imaging.

53 bromide, ethidium homodimer, bis-benzimide (DAPI), Hoechst 33258 and thiazole orange] to function as

54 KD values quantitate binding effects between DAPI and the native and analogue sequences.

55 re labeled with the DNA minor groove binder, DAPI, followed by measurement and imaging of the fluores

56 API, BrdU/GFAP/HNA/DAPI, Ngn1/DAPI, and BMP4/DAPI were measured by immunofluorescence staining; Shh,

57 d dsDNA results in displacement of the bound DAPI, producing a decrease in the observed fluorescence.

58 ned a probe composed of a DNA-binding PS (Br-DAPI) and a photosensitizing photocage (WinterGreen).

59 nglet oxygen mainly in the cytosol, while Br-DAPI localizes to nuclei, binds DNA, and produces ROS us

60 apoptosis by antibody to Fas was analyzed by DAPI staining and electron microscopy.

61 that contributes to minor groove binding by DAPI.

62 uclear morphologic changes of fixed cells by DAPI fluorescence microscopy and reactive oxygen species

63 AdpHyde transduced cells as demonstrated by DAPI (4', 6-diamino-2-phenylindole), TUNEL (terminal deo

64 mplete chromosome separation, as detected by DAPI-bridges and UFBs, while severe HDR disruption addit

65 went apoptosis within 48 h, as determined by DAPI staining.

66 untered by an opposite deflection induced by DAPI binding, thus effectively neutralizing intrinsic cu

67 r the RecA protein-dsDNA complex measured by DAPI displacement is 3 bp per RecA protein monomer in th

68 in livers from fat-1 animals, as measured by DAPI-staining.

69 emoved GalNAz-labeled surface cells, causing DAPI labeling (permeabilization) of underlying cells.

70 A proof-of-concept DAPI interface was designed and characterized using a mi

71 f phenotypic CTCs (EpCAM(+), Cytokeratin(+), DAPI(+), CD45(-)/CD66b(-)) in all patients with metastat

72 is excluded from both the XIST RNA and dense DAPI staining.

73 trates were designed with sequence-dependent DAPI-binding sites to which base excision repair glycosy

74 ,6-diamidine-2-phenylindole dihydrochloride (DAPI) and imaged with a fluorescence microscope.

75 ,6-diamidino-2-phenylindole dihydrochloride (DAPI), to detect proteinaceous and microbial contaminati

76 6-diamidino-2-phenylindole, dihydrochloride (DAPI) staining, and phagosome-lysosome fusion was scored

77 6-diamidino-2-phenylindole, dihydrochloride (DAPI).

78 es of anaphase bridges can be distinguished: DAPI-positive chromatin bridges and DAPI-negative ultraf

79 nges of the other two ligands, using the DNA-DAPI complex as the fluorescence reporter.

80 ning of the same tissue with the nuclear dye DAPI was used to represent virtual slides and a ground t

81 y counterstaining the sections with DNA dye (DAPI), and cell borders can be visualized with a dye-cou

82 Cellular removal was evaluated by H&E, DAPI and DNA quantification.

83 sites since the oligo A/T binding sites for DAPI and Hoechst were centered on the same nucleotide po

84 classical metrics (qPCR, plaque assay, FVIC, DAPI) and outperformed most of them to reveal new biolog

85 mimicking phosphorylation, which shows high DAPI uptake rate without MS stimulation or cleavage of t

86 BrdU/beta-tubulin/HNA/DAPI, BrdU/GFAP/HNA/DAPI, Ngn1/DAPI, and BMP4/DAPI were measured by immunofl

87 BrdU/beta-tubulin/HNA/DAPI, BrdU/GFAP/HNA/DAPI, Ngn1/DAPI, and BMP4/DAPI were

88 S)-induced activation-measured by changes in DAPI uptake rate-was drastically reduced by either knock

89 e were incubated at 4 degrees C overnight in DAPI solution.

90 let state of 4',6-diamidino-2-phenyl indole (DAPI) and its complexes with the oligonucleotides [d(CGA

91 iscontinuous atmospheric pressure interface (DAPI) has allowed the transfer of ions from atmospheric

92 iscontinuous atmospheric pressure interface (DAPI).

93 scontinuous atmospheric pressure interfaces (DAPI).

94 BPMS is mainly expressed in medium and large DAPI-, DRAQ5-, NeuroTrace- and NeuN-stained cells in the

95 DNA binding reactions involving the ligands DAPI, netropsin, lexitropsin, and the lambda repressor b

96 with minor groove and intercalating ligands: DAPI, Hoechst, and ethidium bromide.

97 e quantifications of immunostaining markers (DAPI, amylase, and cytokeratins; Spearman correlation sc

98 irmed by serial section electron microscopy, DAPI, gammaH2AX and phospho-H3 staining.

99 e used to precisely immobilize the molecules DAPI, Hoechst, and netropsin at defined positions in the

100 via CellSearch (EpCAM(pos)/CK(pos)/CD45(neg)/DAPI(pos)) and subsequent FACS sorting.

101 al agents including distamycin A, netropsin, DAPI, Hoechst 33258, and berenil is described.

102 a-tubulin/HNA/DAPI, BrdU/GFAP/HNA/DAPI, Ngn1/DAPI, and BMP4/DAPI were measured by immunofluorescence

103 Markers for nuclear (DAPI), cell type (vimentin, aSMA) and healing (Cx43, TGF

104 uorescently labeled with markers for nuclei (DAPI; 4',6'-diamino-2-phenylindole), endothelial cells (

105 or the lower triplet state energy, E(00), of DAPI.

106 along the circular element in the absence of DAPI but assembled onto the nonbent flanking sequence in

107 t was responsible for the enhanced action of DAPI.

108 The affinity of DAPI for the binding site decreases with the increasing

109 Trapped complexes impeded the association of DAPI in a manner dependent on the enzyme used and the lo

110 Weaker binding of DAPI to phosphate is also detected, and the triplet stat

111 The binding of RecA protein to a complex of DAPI and dsDNA results in displacement of the bound DAPI

112 dichroic components, including detection of DAPI-stained Leishmania parasite without using excitatio

113 According to the model, the short length of DAPI and its absolute specificity for A/T bps with narro

114 hibition of tau aggregates with <25% loss of DAPI-positive cell nuclei underwent further confirmation

115 In addition, morphology of DAPI stained cells and DNA fragmentation assay using gel

116 hibit an increased TM due to the presence of DAPI bound in the minor groove.

117 In the presence of DAPI, most of the duplexes exhibit an increased TM due t

118 expressing mCx30.2 exhibited higher rates of DAPI uptake than did cells expressing any of the other c

119 Automated image segmentation of DAPI-stained nuclei was generated to isolate thousands o

120 heterochromatin in mouse cells and sites of DAPI-dense intranuclear heterochromatin in human and ham

121 The dendritic trees of DAPI-3 cells, which range from about 150 microm up to ab

122 In Ca(2+)-free medium, uptake of DAPI by HeLaCx30.2-EGFP cells was increased approximatel

123 The use of DAPI provides a simple solution to the problem of coupli

124 and classifying egg chamber stages based on DAPI images.

125 staining and counterstaining with Hoechst or DAPI.

126 amples were mounted with propidium iodide or DAPI.

127 the binding to either duplex of netropsin or DAPI induces little or no change in the electrophoretic

128 CN(-) signals overlapped DAPI fluorescence signals corresponding to nuclei.

129 ',6-diaminidin-2-phenylindoldihydrochloride (DAPI).

130 arly show that 4,6-diamidino-2 phenylindole (DAPI) is superior to both of these drugs at negating the

131 s stained with 4,6-diamidino-2-phenylindole (DAPI) [5].

132 estriction of 4',6-diamidino-2-phenylindole (DAPI) binding to packaged DNA.

133 ingrowth with 4',6'-diamino-2-phenylindole (DAPI) filters.

134 Staining with 4',6-diamidino-2-phenylindole (DAPI) indicated that poly- phosphate (polyP) was prefere

135 -groove-bound 4',6-diamidino-2-phenylindole (DAPI) originates from an intricate interplay between the

136 junction with 4'6'-diamidino-2-phenylindole (DAPI) staining and by fluorescence staining of the nucle

137 mCherry with 4',6-diamidino-2-phenylindole (DAPI) staining and green fluorescent protein without DAP

138 gmentation by 4, 6-diamidino-2-phenylindole (DAPI) staining.

139 ide (PI) and 4', 6-diamidino-2-phenylindole (DAPI) staining.

140 6-Diamidino-2-phenylindole (DAPI) was used for nuclear counterstaining.

141 netropsin and 4,6-diamidino-2-phenylindole (DAPI), and a DNA hairpin having the sequence 5'-d(CGAATT

142 of cells with 4,6-diamidino-2-phenylindole (DAPI), and the polyP-binding domain of Escherichia coli

143 toxin (Cdt), 4',6-diamidino-2-phenylindole (DAPI), human gingival epithelial cells (HGEC), human gin

144 tissue (CT), 4',6-diamidino-2-phenylindole (DAPI), human gingival epithelial cells (HGEC), human gin

145 in vitro with 4',6-diamidino-2-phenylindole (DAPI), intracellular injection with Lucifer Yellow, and

146 4',6-diamidino-2-phenylindole (DAPI), netropsin, and pentamidine are minor groove binde

147 equally with 4',6-diamidino-2-phenylindole (DAPI), suggesting that byr4 is required for proper karyo

148 netropsin and 4',6-diamidino-2-phenylindole (DAPI), two AT-specific minor groove binding ligands that

149 in (FLU), and 4',6-diamidino-2-phenylindole (DAPI), was achieved with this self-powered drug-release

150 rescence of a 4',6-diamidino-2-phenylindole (DAPI)-dsDNA complex upon RecA protein binding.

151 uorescence of 4',6-diamidino-2-phenylindole (DAPI)-dsDNA complexes.

152 been defined: 4,6-diamidino-2-phenylindole (DAPI)-sensitive foci that are bound to DNA and DAPI-inse

153 iodide (PI) or 4',6'-diamino-2-phenylindole (DAPI)-stained cells.

154 ur results from 4, 6-diamino-2-phenylindole (DAPI)-stained spreads showed that the "synizetic knot",

155 ton imaging of 4',6'-diamino-2-phenylindole (DAPI)-stained tissue to quantify neuron loss in postmort

156 e stained with 4',6'-diamino-2-phenylindole (DAPI).

157 h the DNA dye 4',6-diamidino-2-phenylindole (DAPI).

158 ide (TP3) and 4',6-diamidino-2-phenylindole (DAPI).

159 meable dye and 4',6'-diamino-2-phenylindole (DAPI).

160 ide (PrI) and 4'-6-diamidino-2-phenylindole (DAPI).

161 roove binder, 4',6-diamidino-2-phenylindole (DAPI).

162 inding ligand 4',6-diamidino-2-phenylindole (DAPI).

163 roplasts with 4',6-diamidino-2-phenylindole (DAPI); staining at the single-molecule level with ethidi

164 py genes, and 4'-6-diamidino-2-phenylindole (DAPI-) and G-banded chromosomes and report nonrandom cyt

165 a DNA-ligand (4',6-diamidino-2-phenylindole, DAPI) system in this work highlight the ease of use and

166 cidification by application of CO(2) reduced DAPI uptake by HeLaCx30.2-EGFP cells but had little effe

167 ement of signals relative to high-resolution DAPI or G-bands.

168 ified on the basis of morphology and reverse DAPI (rDAPI) banding.

169 arkers for incomplete chromosome separation: DAPI-bridges and Ultra-fine bridges (UFBs).

170 in, showed statistically significant shorter DAPI lifetime values than the rest of the chromosomes.

171 ferentially localized to regions with strong DAPI signals.

172 c effects of nuclear stains, including SYTO, DAPI dilactate, Hoechst 33342, and FITC dyes upon the ph

173 The DAPI potentially would allow a significant enhancement t

174 The DAPI ties the DNA of the cell nucleus, emitting blue flu

175 ve human melanoma cells is detectable in the DAPI channel at low micromolar concentrations.

176 Differences in the DAPI lifetimes for the heteromorphic regions suggest dif

177 This target is the DAPI-3 cell.

178 The simplicity of application of the DAPI for performing ion/molecule and ion/ion reactions h

179 ase in the zero-field splitting (zfs) of the DAPI triplet state.

180 The perikarya of the DAPI-3 cells are found in the proximal inner nuclear lay

181 Because the cell bodies of the DAPI-3 cells are the only ones in the inner nuclear laye

182 t on the enzyme used and the location of the DAPI-binding site in relation to the lesion.

183 RNA territory, which resides outside of the DAPI-dense Barr body.

184 of a standard Frenkel exciton theory of the DAPI-DNA interactions.

185 lesser extent, UTP and dCTP also support the DAPI displacement reaction, but dGTP, GTP, dITP and TTP

186 Thus, we have shown that the DAPI-3 cells contain the highest concentrations of the a

187 In contrast to the DAPI-3 cell, we have also shown that the starburst amacr

188 ABA(A) receptor subunits is localized to the DAPI-3 type amacrine cell.

189 pray ionization source synchronized with the DAPI has been implemented to improve the sample usage ef

190 The pressure variation associated with the DAPI operation was used to turn on and off the synchroni

191 for mutant RecA proteins, suggest that this DAPI displacement assay monitors formation of the high a

192 The Delta371hPanx1 HC was permeable to DAPI and Etd(+), but not to propidium iodide, whereas fl

193 , whereas fl-hPanx1 HC was only permeable to DAPI.

194 meter with a DAPI-RIT (rectilinear ion trap)-DAPI configuration has been developed to explore this po

195 d rats transfected with hCx31.9-EGFP took up DAPI and ethidium bromide 5-10 times faster than wild-ty

196 crophages were scored for apoptosis by using DAPI (4',6-diamindino-2-phenylindole dihydrochloride) an

197 ometry, as well as chromosome counting using DAPI staining and fluorescence in situ hybridization, re

198 155 fluorescent dye molecules per CPMV using DAPI (4',6-diamidino-2-phenylindole dihydrochloride), pr

199 ned for nucleus and actin distribution using DAPI and phalloidin respectively.

200 referentially localized to regions with weak DAPI signals.

201 TP3 binds DNA by intercalation, whereas DAPI exhibits minor groove binding.

202 urvival and proliferation were assessed with DAPI staining and Ki67 immunofluorescence staining, resp

203 methyl groups exhibit very poor binding with DAPI, while those containing a single fluorine behave es

204 relocalization of C/EBP beta coincides with DAPI staining of heterochromatin.

205 to 16 fluorescent foci that colocalize with DAPI (4',6'-diamidino-2-phenylindole)-positive material

206 tudies showed the colocalization of KIT with DAPI-stained nuclei in GIST cells without knowing the ro

207 branes were embedded in mounting medium with DAPI (ABCAM, Cambridge, UK) and analysed at 1-, 2-, 3-,

208 neurons with Fluoro-Jade C, cell nuclei with DAPI and activated astrocytes with GFAP immunofluoresenc

209 cent digital images of sections stained with DAPI and antibodies directed against GS-NEM, glial fibri

210 of Arabidopsis thaliana nuclei stained with DAPI or Hoechst.

211 aining and green fluorescent protein without DAPI staining images.