ライフサイエンスコーパス: DEF

10 s and gene activity interact, we exploited a DEF allele which carries a transposon insertion in the M

11 lly assembled on the galactose residues of a DEF chimera.

12 is dependent on DEF-1 GAP activity, since a DEF-1 mutant lacking the GAP domain failed to stimulate

13 80 microM observed for the natural activator DEF.

14 s were performed with PBO (4%), DEM (8%) and DEF (0.25%) and several pyrethroid-resistant markers wer

15 not differ significantly between the ABC and DEF groups (mean [SD], -1.19 [0.49] vs -1.17 [0.48] mug/

16 he rest day and at the completion of BAL and DEF, participants consumed a beverage containing 3.8 mg

17 purified mutant ELK1 in which the D-box and DEF motifs were disrupted did not bind AR.

18 ts showed impaired performance while CON and DEF rats were unaffected.

19 on the ERK2 substrate binding domains, D and DEF binding pocket (DBP).

20 served in An. funestus after to PBO, DEM and DEF whereas a trend for a decreased mortality was observ

21 tes can be classified into DEF-dependent and DEF-independent substrates.

22 ovel LFY/FLO paralogs, here we clone FLO and DEF duplicates from additional Lamiales taxa and apply c

23 for preservation of co-orthologs of FLO and DEF in Lamiales, including interactions among the genes

24 Exposure to PBO and DEF resulted in a broad transcriptional response and abo

25 Similarly, PLE and DEF/GLO induce DNA bending although the direction of the

26 NbDEF is a functional homolog of Antirrhinum DEF.

27 fied duplicates of the genes LFY/FLO and AP3/DEF that directly interact in a floral regulatory pathwa

28 h duplicates of MADS box genes including AP3/DEF are common throughout the angiosperm lineage, LFY/FL

29 from a general increase in cell motility, as DEF-1-expressing cells also exhibit enhanced levels of b

30 dy the function of the Nicotiana benthamiana DEF ortholog (NbDEF).

31 rs, which partially inhibit p38alpha binding DEF-dependent substrates, whereas maintaining its other

32 Moreover, both DEF and PBO induced genes that are strongly implicated i

33 leotide substitutions for one (FLO) or both (DEF) paralogs that appears to be due to relaxed purifyin

34 Most importantly, free and RI-bound DEF-RNase A have pKa values of 6.68 and 7.29, respective

35 g that spreading and motility are altered by DEF-1 through different pathways.

36 In contrast, protection by DEF binding to ERK2 revealed a distinct hydrophobic pock

37 val of the formyl group in these proteins by DEF would explain the rescue of the slow-growth phenotyp

38 ontrast, the inhibition of cell spreading by DEF-1 was not dependent on GAP activity, indicating that

39 n of truncated forms of otoferlin (C2-EF, C2-DEF, and C2-ACEF) can partially rescue the fast and tran

40 ntroduction of the mini-otoferlins C2-EF, C2-DEF, or C2-ACEF allowed us to uncover and characterize f

41 i-otoferlin containing C2-ACEF, C2-EF, or C2-DEF partially restores the fast exocytotic component in

42 t switch, where GAL4 DBD was fused to CfEcR (DEF) and VP16 AD was fused to MmRXR (EF) was found to be

43 luble, dimeric peptide MHC class II chimera (DEF) induces Ag-specific T cell anergy.

44 mpatibility complex (pMHC) class II chimera (DEF) to prediabetic double-transgenic mice prevents the

45 trol diet (CON) or one deficient in choline (DEF) during embryonic days 12-17 were given multiple inj

46 hat were given a deficient level of choline (DEF=0.0 g/kg), sufficient choline (CON=1.1 g/kg) or supp

47 region of Chz1 (Chz1-C) harbors a conserved DEF/Y motif, which reflects the consecutive D/E residues

48 ignificantly, several protooncogenes contain DEF domains and are regulated by ERK1/2.

49 1/2)-MAPKs localize to effectors containing DEF (docking site for ERK, (F)/(Y) -X-(F)/(Y) -P)- or D-

50 MAPK interaction with substrates containing DEF sites.

51 selection--these include the major defensin (DEF) gene cluster and MCPH1, a gene that may have contri

52 of floral homeotic genes such as DEFICIENS (DEF) in non-model systems.

53 We isolated PTD, a member of the DEFICIENS (DEF) family of MADS box transcription factors, from the

54 The DEFICIENS (DEF) gene is required for establishing petal and stamen

55 ere exposed to a choline chloride deficient (DEF), sufficient (CON), or supplemented (SUP) diet durin

56 energy expenditure to induce energy deficit (DEF).

57 icipants in energy balance (BAL) or deficit (DEF) on circulating and skeletal muscle miRNAs.

58 sion of the E. coli polypeptide deformylase (DEF), which removes the formyl group from the N-terminal

59 2',7'-Diethylfluorescein (DEF) has spectral properties similar to those of fluores

60 pyrazolyl) (H2BDP) in N,N'-diethylformamide (DEF) at 130 degrees C generates the metal-organic framew

61 H(2)BDC), and occluded N,N-diethylformamide (DEF) (if any) gave values of 78.64 +/- 2.95 and 99.47 +/

62 Similar reaction in N,N-diethylformamide (DEF) at higher temperatures resulted in a porous, 3-D fr

63 Mn(NO(3))(2).4H(2)O in N,N-diethylformamide (DEF) produces the two-dimensional framework solids Mn(3)

64 ylformamide (DMF), and N,N-diethylformamide (DEF), we can observe, intercept, and isolate, the import

65 have established that a IVa2 protein dimer (DEF-B) binds specifically to an intragenic ML promoter s

66 Muscle glycogen declined during DEF and was preserved during BAL (-188 179 mmol/kg, P-ad

67 , such that hepcidin was 108% greater during DEF compared with BAL (7.8 12.2 ng/mL, P-adjusted < 0.00

68 erleukin-6, plasma hepcidin increased during DEF but not BAL, such that hepcidin was 108% greater dur

69 Here we report that ectopic DEF-1 enhances cell migration toward PDGF as well as IGF

70 to an altered binding of the nonreducing-end DEF trisaccharide to the native serpin conformation.

71 Therefore, disrupting ERK-DEF domain interactions could be an alternative to inhib

72 ERK2 DEF mutants undergo regulated nuclear translocation but

73 Thus, an apparent critical role for ERK2 DEF motif signaling during tumorigenesis is the regulati

74 In a second experiment, DEF, CON, and SUP rats were either maintained on a contr

75 ated whether NIH 3T3 cells stably expressing DEF-1 have altered cell motility.

76 ar factor, digestive organ expansion factor (DEF), has a key role in ribosome biogenesis, functioning

77 tinin (HA)110-120/I-E(d)alphabeta/Fcgamma2a (DEF).

78 We present an automated dead-end filling (DEF) approach, which is derived from the wisdom of endos

79 Consistent with these findings, DEF is highly expressed in human neuroblastoma, and its

80 ns result from reduced diesel exhaust fluid (DEF) usage due to lower NO(x) emissions.

81 Further evidence for a role for DEF-1 in adipogenesis was provided by heightened express

82 DEJL motifs), RSK-1 (DEJL motif), and c-Fos (DEF motif) with K(D) values of 0.25, 0.15, and 0.97 muM,

83 Recovery from DEF anergy occurred late and spontaneously at the expens

84 receptor constructs were prepared by fusing DEF domains (hinge region plus ligand-binding domain) of

85 , several constructs were prepared by fusing DEF domains of Choristoneura fumiferana EcR (CfEcR), C.

86 (D-domain) and a docking site for ERK, FXF (DEF) motif (F-site), respectively.

87 ntain functional docking site for ERK, FXFP (DEF) domains that serve to locally concentrate the activ

88 equired the hBVR docking site for ERK, FXFP (DEF, C-box) and D(delta)-box (ILXXLXL) motifs.

89 ng proteins contain amino acid motifs, e.g., DEF or D-motifs, which regulate docking with ERK1/2.

90 However, DEF-1 mRNA was detected in multiple tissues, suggesting

91 ral (HSET), and other genes (TULP1, HSPRARD, DEF-6, EO6811, cyclophilin), as well as a number of RP g

92 tahedral SBUs found in Zn(2)(NDC)(3).[(HTEA)(DEF)(ClBz)](2) (MOF-37) form the most common structure f

93 ectly accessing the chloropeptin I versus II DEF ring system as well as key unnatural isomers, its ut

94 a decrease in dentate cell proliferation in DEF rats only.

95 Variations in DEF site sequence requirements provide one possible mech

96 g p38alpha substrates can be classified into DEF-dependent and DEF-independent substrates.

97 ogous substrate used to provide the isolated DEF ring system in our first-generation approach.

98 d to a choline-supplemented diet (5.0 g/kg), DEF rats' performance was significantly impaired while C

99 Physiological ligands like DEF chimeras may provide new tools for silencing the aut

100 In addition, mutating DEF pocket residues decreased the autophosphorylation ca

101 basepairs) which eukaryotes require obeys N(DEF)=1/2 (N(C)/N(P)) (N(C)-N(P)), where N(C) is the amou

102 This value N(DEF) corresponds to a few percent of the genome in Homo

103 h D domains, prevents RSK activation but not DEF-domain signaling.

104 -domain binding pocket prevent activation of DEF-domain-containing effectors but not RSK (90 kDa ribo

105 ing an assay for the DNA-binding activity of DEF-A, we identified the unknown protein by using conven

106 Administration of DEF in vivo induced differentiation of resting and activ

107 Administration of DEF-gal-Dox to mice expressing the TCR-HA transgene redu

108 moter with the specificity characteristic of DEF-A and stimulates transcription from the ML promoter

109 l L4 33-kDa protein is the only component of DEF-A: the IVa2 and L4 33-kDa proteins are necessary and

110 The antidiabetogenic effects of DEF rely on the induction of anergy in splenic autoreact

111 The recalls of reactions and dead ends of DEF reach around 73% and 86%, respectively.

112 Expression of DEF in a subset of meristem layers gives rise to organs

113 of these chimeras we show that expression of DEF in L1 makes a major contribution to morphology in wh

114 Ectopic expression of DEF-1 in fibroblasts resulted in the differentiation of

115 sis was provided by heightened expression of DEF-1 mRNA in adipose tissue isolated from obese and dia

116 bly because of a non-autonomous inhibitor of DEF activity in this whorl.

117 s, reflecting altered patterns and levels of DEF gene activity.

118 ereas, coexpression of an inactive mutant of DEF does not.

119 Since transient overexpression of DEF-1 has been shown to interfere with focal adhesion fo

120 Recombinant proteins containing the RRMs of DEF-3(g16/NY-LU-12) and LUCA15 specifically bound poly(G

121 a from Hu syndrome patients with the RRMs of DEF-3(g16/NY-LU-12) and LUCA15.

122 ression patterns are more similar to that of DEF and AP3 than to other members of the TM6 subfamily.

123 alysis indicates that the price and usage of DEF have the largest impacts on TCA reduction.

124 he increase in cell motility is dependent on DEF-1 GAP activity, since a DEF-1 mutant lacking the GAP

125 xpenditures in BAL (689 +/- 852 kcal/day) or DEF.

126 eu (DEJL) and a second containing Phe-X-Phe (DEF).

127 such as S,S,S-tributyl phosphorotrithioate (DEF), diethyl maleate (DEM), piperonyl butoxide (PBO) an

128 ch proteins in Antirrhinum majus, SQUA, PLE, DEF and GLO.

129 defined as the DEF site interaction pocket (DEF pocket), is formed subsequent to ERK2 and p38alpha a

130 L) (amino acids 41-97) harbors two potential DEF-type ERK1/2 kinase-docking domains, DEF1 and DEF2.

131 We have purified and cloned a novel protein, DEF-1 (differentiation-enhancing factor), from bovine br

132 We show that MAPK isoforms recognize DEF sites with unique sequences and identify two key res

133 An improved synthesis of rings DEF of solanoeclepin A has been achieved from ent-Hajos

134 of the parameters evaluated (MRSE, CYL, RMS, DEF, COMA, TREF, and SA).

135 Mutating selected DEF pocket residues significantly decreased the phosphor

136 a platform for designing p38alpha-selective DEF site blockers, which partially inhibit p38alpha bind

137 ocess allowed access to the first simplified DEF ring analogues of the kibdelones.

138 -activated receptor gamma (PPARgamma), since DEF-1 NIH 3T3 cells demonstrated augmented levels of PPA

139 esponse occurred upon interaction of soluble DEF with TCR and CD4 molecules followed by early activat

140 Independent of antigen processing, soluble DEF was almost 2 logs more potent in stimulating cognate

141 ave previously demonstrated that the soluble DEF molecule binds stably and specifically to HA110-120-

142 nsically active p38alpha mutants, suggesting DEF-mediated trans-autophosphorylation in p38alpha.

143 Perhaps somewhat surprisingly, DEF rats were not more susceptible to seizure induction

144 h a dose enhancement factor at 10% survival (DEF(10)) of 1.310 and 1.365 for KPC and Panc02 cell line

145 ional infected-cell-specific protein, termed DEF-A, to the promoter.

146 cell-based ARF GAP assay to demonstrate that DEF-1 functions as a GAP for ARF1 and not ARF6 in vivo.

147 At 70 days of age, we found that DEF and SUP rats required fewer choices to locate 8 bait

148 Our results indicate that DEF and possibly other components of the SSU processome

149 These results suggest that DEF-1 is an important component of a signal transduction

150 This suggests that DEF-1 alters cell motility through the deactivation of A

151 The DEF-gal-Dox construct preserved both the binding capacit

152 ERK-docking sites in ELK1, the D-box and the DEF (docking site for ERK, FXFP) motif, as the essential

153 ining the DNA-binding domain of GAL4 and the DEF segment of TR2-11.

154 ion between the ABC-flavanone moiety and the DEF-flavone moiety, as well as the electronic transition

155 A second docking site, defined as the DEF site interaction pocket (DEF pocket), is formed subs

156 ntrast to HA110-120 peptide presented by the DEF molecule to T cells, the nominal synthetic peptide i

157 thrombin conformation that is induced by the DEF trisaccharide.

158 n alternative mode of action mediated by the DEF-binding pocket (DBP) of ERK.

159 Mutations in the DEF-domain binding pocket prevent activation of DEF-doma

160 ment) and a tricyclic quinone monoketal (the DEF-ring segment).

161 een to identify sequence requirements of the DEF site (docking site for ERK FXF), a docking motif sep

162 Construction of the DEF-ring system of nogalamycin and menogaril has been ac

163 largely uncharacterized TM6 subfamily of the DEF/APETELA3(AP3)/TM6 group, its spatio-temporal express

164 To prepare the DEF-ring segment for annulation, a mild dearomatization

165 d of intrinsically active mutants showed the DEF pocket, giving motivation for studying its role in s

166 pling to connect the AB ring system with the DEF ring system, and a Nazarov cyclization to construct

167 ive silencing activity is encoded within the DEF segment of the receptor molecule, as evidenced by th

168 ety and the electronic transition within the DEF-flavone moiety, while another diagnostic positive CE

169 well as the electronic transition within the DEF-flavone moiety.

170 Thus, DEF-3(g16/NY-LU-12) and LUCA15 represent members of a no

171 Brief exposure of HA-specific T cells to DEF-gal-Dox construct in vitro was followed by drug inte

172 In contrast to DEF, a combination of TCR and CD4 mAbs did not induce su

173 ure, but converts back to 1 upon exposure to DEF, consistent with the presence of a flexible framewor

174 Remarkably, ERK2 signaling to DEF motif-containing targets is required to induce EMT a

175 degree of substrate preference was unique to DEF-1, as other ARF GAP proteins, ACAP1, ACAP2, and ARFG

176 nteraction profile for ECS and trisaccharide DEF.

177 as previously shown to involve trisaccharide DEF first binding and inducing activation of the serpin,

178 M) comparable to the reference trisaccharide DEF ( approximately 4.5 microM), it accelerated the inhi

179 nding domain for the reference trisaccharide DEF.

180 mimic the nonreducing end trisaccharide unit DEF of the sequence specific heparin pentasaccharide DEF

181 Using DEF-RNase A rather than fluorescein-RNase A in a micropl

182 After 12 weeks, DEF rats were significantly impaired by choline suppleme

183 the signal transduction pathway(s) in which DEF-1 is involved is not limited to adipogenesis.

184 ope appearance at 120 min was 74% lower with DEF (59 38% change from 0 min) and 49% lower with BAL (1

185 to the approximately 300-fold observed with DEF.