ライフサイエンスコーパス: DNA library

1 or forming what has been called a 'satellite DNA library'.

2 or random clones from a small insert genomic DNA library.

3 and used to a probe a P. funiculosum genomic DNA library.

4 nding sequence from a human single-copy (sc) DNA library.

5 but one of these classes from a chromosomal DNA library.

6 eta, were isolated from a Paramecium genomic DNA library.

7 eening a recombinant M. tuberculosis genomic DNA library.

8 ruct, screen and finally clone a gene from a DNA library.

9 a1 gene was isolated from a human P1 genomic DNA library.

10 f strain AG84-3, was isolated from a genomic DNA library.

11 oding gene was isolated from a human genomic DNA library.

12 ces in GenBank and from inserts in a genomic DNA library.

13 with next-generation sequencing of a genomic DNA library.

14 nd-read drop-IVT2H method to screen a random DNA library.

15 organs, and used to generate a complementary DNA library.

16 ed fluorescence compared with the unselected DNA library.

17 using fosmids selected from a total cellular DNA library.

18 tilize nucleotide content changes to produce DNA libraries.

19 ubtracted thymus cDNA library and in genomic DNA libraries.

20 total DNA instead of cloning fragments from DNA libraries.

21 able elements to be present in complementary DNA libraries.

22 ication by extensive screening of randomized DNA libraries.

23 e discovered within 3 days using preselected DNA libraries.

24 ust techniques for enhanced amplification of DNA libraries.

25 , using it to deplete unwanted sequence from DNA libraries.

26 ement to use global mutagenesis or introduce DNA libraries.

27 nd provides a way to preserve the quality of DNA libraries.

28 ly 3 d from flow cytometry to sequence-ready DNA libraries.

29 ired-end sequences derived from large-insert DNA libraries.

30 oxyl radical cleavage data produced from two DNA libraries.

31 Escherichia coli carrying a soil metagenomic DNA library(3) with the lytic coliphage T4 to isolate cl

32 From an H. ducreyi 35000HP genomic DNA library, a clone complementing the defect in A77 was

33 s of other groups using the same recombinant DNA library, a comprehensive map was constructed that in

34 We selected from a DNA library an aptamer specifically recognizing human ep

35 -generation sequencing analysis of mate-pair DNA libraries and applied these tools to 16 PTCL patient

36 phage and P1 phage clones from human genomic DNA libraries and characterized by Southern blot and nuc

37 anism to clone biosynthesis genes from large DNA libraries and greatly facilitate the exploration of

38 We demonstrate the stability of conjugated DNA libraries and related sequencing results over the co

39 w approach could be applied in the design of DNA libraries and result in a more efficient and faster

40 es were prepared into reduced representation DNA libraries and sequenced to identify genomic regions

41 l three assays on a common set of 27 ancient DNA libraries and show that all three are effective at e

42 acterial artificial chromosome mouse genomic DNA library and characterized by polymerase chain reacti

43 se (ADH) was cloned from a Caucasian genomic DNA library and characterized.

44 Also, by using a cosmid DNA library and genomic fractions as hybridization probe

45 Serologic screening of a complementary DNA library and Northern blotting were used to clone the

46 e was transformed with a C. albicans genomic DNA library and screened for the production of active al

47 lysozyme c group were cloned from a genomic DNA library and sequenced.

48 ow-copy sequences were isolated from a human DNA library and tested for their ability to detect restr

49 li strain CS89 was transformed with the same DNA library and then screened for Asp-tRNA(Asp) formatio

50 by sequencing inserts from a CB4856 genomic DNA library and using an informatics pipeline to compare

51 tor of neutrophil elastase into a randomized DNA library and, using the SELEX process, iteratively se

52 We establish methods for barcoding CAGE DNA libraries, and apply them to measure persistence of

53 isms was identified by screening chromosomal DNA libraries, and cloned.

54 in situ hybridization of chromosome-specific DNA libraries, and mapped >2,000 sites of recombination

55 microfluidics workflow for screening random DNA libraries, and represents a novel alternative method

56 cloning complex individual or combinatorial DNA libraries, and routine or high-throughput cloning of

57 t was used to screen a lambda ZAP II genomic DNA library, and an open reading frame expressing a 28-k

58 shortcomings, we have devised a method where DNA libraries are constructed on the surface of microbea

59 DNA libraries are constructed using native genomic DNA f

60 d genes in this region by directly screening DNA libraries as well as by database searching for ESTs.

61 ers, SVCT1 and SVCT2, from rat complementary DNA libraries, as the first step in investigating the im

62 ector ideal for cloning PCR products, making DNA libraries, as well as routine cloning and bacterial

63 d DNA sequencing, hybridization screening of DNA libraries, assembly of gene cassettes, run-off trans

64 s the generation of eukaryotic and bacterial DNA libraries at eukaryotic single-cell level resolution

65 .5.1.9) from an Arabidopsis thaliana genomic DNA library based on the homology between the yeast bios

66 rmation in real time and within minutes of a DNA library being loaded.

67 leavage of a phosphodiester bond in RNA than DNA libraries built from a standard four-nucleotide alph

68 has been cloned from adaptor-ligated genomic DNA libraries by polymerase chain reaction.

69 cloned and used to probe a wild-type Xa23R1 DNA library by colony hybridization.

70 The genes were isolated from a genomic DNA library by complementation of a lipase-deficient tra

71 tein) by screening a HeLa cell complementary DNA library by using the yeast two-hybrid system.

72 e-tag-guided screening of soil environmental DNA libraries can be used to guide the discovery of new

73 Complementary DNA libraries can define the genetic constituents of spe

74 oylphenylalanine, Bpa) amino acids, a single DNA library can be used to incorporate different amino a

75 TF-DNA interactions using mixtures of random DNA libraries carrying different epigenetic modification

76 designed for the propagation of large insert DNA library clones.

77 ed technology that entails construction of a DNA library comprising up to at least 4(7) ( approximate

78 Sequencing of a DNA library constructed from circular plasmid fragments

79 ntaining clones were isolated from a genomic DNA library constructed in our lVET promoter trap vector

80 ms of high molecular weight DNA suitable for DNA library construction and Sanger sequencing.

81 DNA library construction and sequencing were performed u

82 Using improved DNA library construction methods that enrich for DNA fra

83 ery, the currently widely used complementary DNA library construction protocol generates biased seque

84 ng ALK translocations, we combined mate-pair DNA library construction, massively parallel ("Next Gene

85 cations in nanotechnology, gene editing, and DNA library construction.

86 Environmental DNA libraries contain large reservoirs of bacterial gene

87 Two cosmids from an E. coli O157:H7 DNA library contain an adherence-conferring chromosomal

88 of IL-6 SOMAmers were isolated from modified DNA libraries containing 40 random positions and either

89 DNA libraries containing exome sequences tagged with uni

90 ctural families using chemically synthesized DNA libraries containing position-specific 5-methylcytos

91 ctivity, we deep-sequenced the products of a DNA library containing 10 random base-pairs on each side

92 A hairpin DNA library containing an 8-base pair sequence-randomize

93 A DNA library containing DNA-DNA mismatches at PAM + 1 to

94 quencing small fragments of a random genomic DNA library containing one or more modular PKS gene clus

95 Notably, our DNA library contains the genome of a bacterium within th

96 bidopsis thaliana, and man, (3) large-insert DNA libraries: cosmid, YAC and BAC, and genome physical

97 d in a macroarray format to analyze chimeric DNA libraries created by DNA shuffling.

98 ened over one million clones from metagenome DNA libraries derived from sixteen different environment

99 Complex DNA libraries derived from the host-symbiont population

100 roviral clone, pJSRV21, from a tumor genomic DNA library derived from a natural case of SPA.

101 ce within the equilibrium mixture of masking DNA, library DNA, and target cells.

102 Five cosmids from N. meningitidis DNA library enabled a heme-requiring (hemA), HmbR-expres

103 tion gene homologs found within a large soil DNA library enabled the identification and recovery of a

104 eriments were carried out on circular 103-nt DNA libraries encoding 40-nt randomized sequences as wel

105 d shotgun scanning glycomutagenesis in which DNA libraries encoding all possible glycosylation site v

106 a systematic approach in which combinatorial DNA libraries encoding large numbers of random mutations

107 A DNA library encoding potential peptide binders was encap

108 lecules result in the establishment of oligo DNA libraries enriched in DNA molecules containing speci

109 gate amplification mechanisms: sequencing of DNA libraries enriched with tumor-derived palindromic DN

110 ent within GCs, we constructed a recombinant DNA library enriched for cDNAs derived from human genes

111 n 8,000-3,000 years ago by enriching ancient DNA libraries for a target set of almost 400,000 polymor

112 We constructed genomic DNA libraries for AFGP-bearing and AFGP-lacking species

113 fluidic system for preparing sequencer-ready DNA libraries for analysis by Illumina sequencing.

114 ling of bacterial samples and preparation of DNA libraries for next-generation sequencing.

115 inhibit splicing, we searched human genomic DNA libraries for sequences that would inhibit the inclu

116 linking a drug-pharmacophore to a degenerate DNA library for use in cell- and whole-animal systematic

117 harboring members of an F. novicida genomic DNA library, for 4'-phosphatase activity.

118 M-seq method successfully generated reliable DNA libraries from 1-25 ng of mouse and human DNA.

119 Subtracted complementary DNA libraries from highly purified murine fetal liver st

120 We compared human complementary DNA libraries from OA-affected and normal cartilage and

121 re, we constructed directional complementary DNA libraries from plaque-periplaque messenger RNA and a

122 A cosmid DNA library from a PAK strain deleted for xcpP-Z was tes

123 pathway in tea, we generated a complementary DNA library from leaf tissue of the blister blight-resis

124 Analysis of single-cell DNA libraries generated by different technologies reveal

125 are accelerated by integrating high-quality DNA library generation with an ultra-high throughput pro

126 screening an ordered S. typhimurium genomic DNA library, harbored in Escherichia coli K-12, for expr

127 ectors, with which several very large-insert DNA libraries have been developed.

128 the p79 gene as the bait and a yeast genomic DNA library, identified two independent groups of positi

129 vo synthesis and cell-free cloning of custom DNA libraries in sub-microliter reaction droplets using

130 ies using only a single, long-insert shotgun DNA library in conjunction with Single Molecule, Real-Ti

131 was isolated by activity screening a genomic DNA library in Streptomyces lividans TK24.

132 colonies of a R. leguminosarum 3841 genomic DNA library in the host strain R. etli CE3.

133 colonies of an R. leguminosarum 3841 genomic DNA library in the host strain S. meliloti 1021.

134 lection and evolution of proteins encoded by DNA libraries, in which individual nascent proteins (phe

135 egy that combines a synthetically engineered DNA library inserted in each run and a new computational

136 We developed quantitative gene assembly and DNA library insertion into the Saccharomyces cerevisiae

137 sed method of highly efficient site-specific DNA library integration.

138 By transferring an F. novicida genomic DNA library into E. coli and selecting for low level pol

139 niform amplification of complex templates in DNA libraries, introducing unwanted biases.

140 High-throughput transgenesis using synthetic DNA libraries is a powerful method for systematically ex

141 randomization and screening of combinatorial DNA libraries is a powerful technique for understanding

142 ology-based screening of large environmental DNA libraries is likely to permit the directed discovery

143 The resulting DNA library is incubated with an affinity-tagged in vitr

144 ast method for introduction of barcodes into DNA libraries made from 5 ng of DNA.

145 hroughput screening of complex environmental DNA libraries more than 40 novel microbial pectate lyase

146 ternative for construction of small-fragment DNA libraries of defined sequences.

147 pA protein, its gene was cloned from genomic DNA libraries of T. denticola.

148 designated chsC and chsG were isolated from DNA libraries of the opportunistic fungal pathogen, Aspe

149 ere selected in vitro from a single-stranded DNA library of 1.8 x 10(15) oligonucleotides showing dis

150 We have designed an effective search of a DNA library of approximately 10(60) in size, and have id

151 st for relapsing fever, we created a genomic DNA library of B. hermsii, screened transformed Escheric

152 A partial genomic DNA library of Chlamydomonas reinhardtii was screened wi

153 A DNA library of F. nucleatum FDC 364 was constructed by p

154 clone these immunogenic antigens, a genomic DNA library of Nine Mile phase I was screened with conva

155 this sequence were used to screen a genomic DNA library of PG31.

156 A DNA library of pRJ28, a large linear plasmid encoding me

157 to the N terminus of the enzyme to screen a DNA library of Pseudomonas sp. MA-1.

158 , arsA and arsB were isolated from a genomic DNA library of S. ovata.

159 erovar Enteritidis, we transformed a genomic DNA library of SE2472 into SE8743.

160 and cobalt ions was isolated from a genomic DNA library of Staphylococcus aureus RN450.

161 Using tpgR1 to probe a genomic DNA library of Streptomyces lividans ZX7, whose linear c

162 For each, a DNA library of V3 region of bacterial 16S ribosomal RNA

163 dCHS2 was isolated by screening a subgenomic DNA library of Wangiella dermatitidis by using a 0.6-kb

164 preserve RNA structure and to limit all RNA-DNA library oligonucleotide interactions to 1:1 stoichio

165 By sequencing a common DNA library on different sequencers, we demonstrate that

166 nsformants harboring a Campylobacter genomic DNA library, one recombinant plasmid that encoded a heat

167 t and economical strategies to clone complex DNA libraries or variants of biological modules.

168 Single and multiorganism DNA libraries originating from various environments were

169 omain amplicons derived from saturating soil DNA libraries, our analysis pipeline led to the recovery

170 amplification, and the use of the Nextera XT DNA library preparation kit produced significantly bette

171 atic cleavage-based approach, the Nextera XT DNA library preparation kit, for library preparation.

172 es fast and reliable targeted single-nucleus DNA library preparation of 38 samples from 16 pancreatic

173 This method can be easily added to existing DNA library preparation techniques and can improve the a

174 ped a methodology integrating cost-effective DNA library preparation with nanopore sequencing, reduci

175 In combination with single-stranded DNA library preparation, this method enabled us to recon

176 on data, which are routinely acquired during DNA library preparation, to annotate output sequence dat

177 es accessibility and methylation from single DNA library preparations.

178 use WEHI-3 monocytic cell line and a genomic DNA library prepared from 129/SvJ mouse tissue.

179 A genomic DNA library prepared from a benomyl resistant strain of

180 have cloned its gene, gngC, from the genomic DNA library prepared from C. perfringens ATCC10543.

181 , we isolated 14 clones from a complementary DNA library prepared with total RNA extracted from the s

182 Two members of a 10-hairpin DNA library previously found to bind most tightly to the

183 We utilized DNA libraries produced from Mycobacterium tuberculosis c

184 From genomic DNA library production to base-called sequences, this pr

185 While many methods are available for DNA library quantification, there is no unambiguous gold

186 Soil DNA libraries represent large reservoirs of biosynthetic

187 D46 and GTKO porcine aortic EC complementary DNA libraries, respectively.

188 Remarkably, selections employing modified DNA libraries resulted in the first successful isolation

189 genetic information has been 'fished out' of DNA libraries resulting in the discovery of new natural

190 eration sequencing analysis of the assembled DNA library revealed that ligation was accurate, directi

191 Using a complementary DNA library screen, we identified the transcriptional re

192 nesis, mutation detection, DNA modification, DNA library screening and circular DNA production.

193 re we show, using an iterative complementary DNA library screening approach, that human occludin (OCL

194 study, we used a phage display complementary DNA library screening strategy to identify P falciparum

195 t the depth-of-coverage yield of single-cell DNA libraries sequenced at arbitrary depths.

196 nterlacing of multiple messages on synthetic DNA libraries showcases the potential of chemical reacti

197 t to screen a yeast two-hybrid complementary DNA library specific for regenerating livers with massiv

198 rotected mRNA fragments are converted into a DNA library suitable for deep sequencing using a strateg

199 titative conversion of the RNA sample into a DNA library suitable for sequencing.

200 In this study, we look at the error rate of DNA libraries synthesized by photolithography and dissec

201 the form of adapter-ligated, short-fragment, DNA libraries that may be amplified by polymerase chain

202 tion, we conducted a screen of an M. marinum DNA library that provides 2.6-fold coverage of the entir

203 ing radiation, was constructed without using DNA libraries, the polymerase chain reaction, or electro

204 n-for a large randomly generated recombinant DNA library, there exists a staircase of DNA fragments a

205 ce the effective depth of synthesized vector DNA libraries, thereby raising the discovery cost of nov

206 screen several heterologous and metagenomic DNA libraries, thus enlarging the genomic space that can

207 224,180 Illumina reads from 12 complementary DNA libraries to build what is to our knowledge the firs

208 inaceous transdominant inhibitors encoded by DNA libraries to cause mutant phenocopies may facilitate

209 Most techniques use PCR to amplify DNA libraries to obtain sufficient quantities for optica

210 gainst standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins an

211 DNA aptamers are selected from a DNA library to bind intact infectious, but not noninfect

212 f aptamers have been evolved from an initial DNA library to characterize target cells at the molecula

213 A-approved drug Pluvicto to a degenerate N40-DNA library to direct the selection of aptamers against

214 : 1) in an aptamer evolved from a six-letter DNA library to selectively bind liver cancer cells; and

215 A), short barcodes are counted by sequencing DNA libraries transfected into cells and the cell's outp

216 of human PAX6 from single-copy human genomic DNA libraries using cyclic amplification of protein bind

217 with subnanomolar affinity from a randomized DNA library using in vitro selection.

218 ected a few DNA aptamers by immobilizing the DNA library using oxytetracycline as the target.

219 The screening of an archived soil DNA library using primers designed to target oxytryptoph

220 High throughput screening of microbial DNA libraries was used to identify alpha-amylases with p

221 An A. salmonicida genomic DNA library was constructed by using lambda GEM-11 and r

222 n of the crab MIH and MO-IH genes, a genomic DNA library was constructed from DNA of an individual fe

223 An H. pylori DNA library was cotransformed into SE5000 (pHP8080) and

224 A large-insert domestic cat genomic DNA library was developed using a P1 artificial chromoso

225 ed from a P. radiata (Monterey pine) genomic DNA library was found to possess all of the sequence fea

226 A fosmid DNA library was prepared from a marine picoplankton asse

227 A lambda ZAP II genomic DNA library was screened with the pool of antisera to or

228 A new hairpin DNA library was selected based on affinity for immobiliz

229 OD gene isolated from a normal human genomic DNA library was used to determine the DNA sequence of Mn

230 tinylated RNA baits transcribed from genomic DNA libraries, we are able to capture DNA fragments from

231 By rational design of synthetic DNA libraries, we first evolve neurotensin receptor 1 fo

232 functional screen of mammalian complementary DNA libraries, we identified moesin as a novel gene whos

233 ing of a murine choroid plexus complementary DNA library, we identified a new chemokine, designated n

234 From an N. coriiceps testicular DNA library, we isolated a 13.8-kilobase pair genomic cl

235 ter 15 rounds of selection with a structured DNA library, we were able to isolate SRAs that possess l

236 igonucleotide sequences of a full complexity DNA library well below the value for the dissociation co

237 hese painting probes and their corresponding DNA libraries were developed by chromosome micromanipula

238 Genomic DNA libraries were generated from one miniature swine th

239 Woodchuck cDNA and genomic DNA libraries were screened with woodchuck-specific DNA

240 Complementary DNA libraries were sequenced by Solexa.

241 the RRBS method failed to generate reliable DNA libraries when using <2 ng of DNA, the RREM-seq meth

242 h to interrogate Sox2/Pax6 dimerization on a DNA library where five positions of the Pax6 half-site w

243 typically requires screening sizable random DNA libraries, which limits the designs to span merely a

244 ts from comparative experiments suggest that DNA libraries with increased chemical diversity, higher

245 ing data for both human and Escherichia coli DNA libraries with negligible coverage bias compared to

246 ermore, by constructing and screening random DNA libraries with the tet expression shuttle system, 78

247 Probing of a human complementary DNA library with anti-Ma serum resulted in the cloning o

248 uffling method to create a customized random DNA library with flexible sequence design and length.

249 The screening of a genomic DNA library with polyclonal antisera raised against nati

250 nnewicki was isolated by screening a genomic DNA library with serum from a mare that had recently abo

251 liformis was isolated by screening a genomic DNA library with serum from a patient with the chronic v