ライフサイエンスコーパス: DNA packaging

1 itical for transduction, capsid assembly, or DNA packaging.

2 sembles sequentially on the viral head after DNA packaging.

3 e mechanisms involved in virion assembly and DNA packaging.

4 empty capsids, indicating a defect in viral DNA packaging.

5 tial for pRNA binding and thereby subsequent DNA packaging.

6 gene regulation, chromatin condensation and DNA packaging.

7 identified bulge residues are important for DNA packaging.

8 FI protein (gpFI), is required for efficient DNA packaging.

9 e three-helix junction in motor assembly and DNA packaging.

10 PhuZ filaments toward the phage nucleus for DNA packaging.

11 UL25 minor capsid proteins are essential for DNA packaging.

12 the assembly of the ring ATPase that drives DNA packaging.

13 lude that the major role of IVa2 is in viral DNA packaging.

14 e loops have been proposed to play a role in DNA packaging.

15 alyzed, thus indicating a profound defect in DNA packaging.

16 membrane and optimal viral DNA synthesis and DNA packaging.

17 is required late in infection, during phage DNA packaging.

18 to discriminate between lambda and 21 during DNA packaging.

19 mational changes can occur in the absence of DNA packaging.

20 d the affects they have on transcription and DNA packaging.

21 capsid, and precluded viral replication and DNA packaging.

22 ence of the DR1 element was also crucial for DNA packaging.

23 al genomes before and after, but not during, DNA packaging.

24 L1 52/55-kDa protein, which is required for DNA packaging.

25 energy to supplement the nanomotor-driven T4 DNA packaging.

26 termediate that was fully active in in vitro DNA packaging.

27 ase prior to subsequent shell maturation and DNA packaging.

28 amics of entire nucleosomes and higher-order DNA packaging.

29 pressure builds up inside the capsid during DNA packaging.

30 rough the portal vertex in the capsid during DNA packaging.

31 nd pUL28 interact with the portal to mediate DNA packaging.

32 ructures rather than having a direct role in DNA packaging.

33 supportive of a topological role in dynamic DNA packaging.

34 odecamers are central to capsid assembly and DNA packaging.

35 ays a key role in the procapsid assembly and DNA packaging.

36 s: the initiation of procapsid formation and DNA packaging.

37 this center lead to a profound loss of phage DNA packaging.

38 flexibility required for capsid assembly and DNA packaging.

39 moved to the surface of the compartment for DNA packaging.

40 not be a static channel at the late stage of DNA packaging.

41 induces DNA compression and rotation during DNA packaging.

42 Maturation of the capsid occurs during DNA packaging.

43 he packaging motor, and for participation in DNA packaging.

44 contribute to stabilizing the P22 capsid for DNA packaging.

45 sembles onto the virion just before or after DNA packaging.

46 structural changes during self-assembly and DNA packaging.

47 esviruses to play an essential role in viral DNA packaging.

48 lge, rather than its sequence, is primary in DNA packaging activity.

49 Asp255 mutants showed no detectable in vitro DNA packaging activity.

50 wild-type (WT) but exhibit little ATPase or DNA packaging activity.

51 e-specific cos cleavage activity, as well as DNA packaging activity; however, the heterotrimer is dep

52 ite a conserved role for histones as general DNA packaging agents, it is now clear that another key f

53 h has the potential to differentially affect DNA packaging among individuals.

54 latory mechanism for coupling DNA cutting to DNA packaging among the headful packaging nucleases from

55 ases into this feature resulted in a loss of DNA packaging and an impairment of initiation complex as

56 y of the initial prohead I form and prior to DNA packaging and capsid expansion.

57 inor capsid proteins of HSV-1, implicated in DNA packaging and capsid maturation.

58 s solfataricus that has been associated with DNA packaging and chromatin regulation.

59 close mechanistic connection in vivo between DNA packaging and developmentally concurrent replication

60 nal surface of capsids and are essential for DNA packaging and DNA retention in the capsid, respectiv

61 ding protein as well as DNA, suggesting that DNA packaging and expulsion of the scaffolding protein a

62 for the pac2 T element in the initiation of DNA packaging and for the GC-rich motifs flanking the pa

63 ated protein that is involved in chromosomal DNA packaging and gene regulatory functions.

64 dle essential for genome stabilization after DNA packaging and implicated in Gram-negative cell envel

65 of the viral single-stranded DNA genome and DNA packaging and may play versatile roles in virus-host

66 oscillation of the head-tail connector upon DNA packaging and release.

67 motor by ensuring the correct orientation of DNA packaging and restricting initiation to a single eve

68 olution to coordinate the transition between DNA packaging and tail assembly.

69 sly that the peptide component mediates both DNA packaging and targeting of the nanoparticle while in

70 between the motor protomers is required for DNA packaging and that incorporation of even a single mu

71 like KUL25NS, had a block at a late stage of DNA packaging and that the packaged genome was shorter t

72 ids and that the C terminus is essential for DNA packaging and the production of infectious virus thr

73 ifying structural basis for coordinating the DNA packaging and transcription repression functions of

74 The dynamics of DNA packaging and unpackaging from the NCP affect all DN

75 he regulation of late gene expression, viral DNA packaging, and infectious virus production.

76 ration, release of scaffolding proteins upon DNA packaging, and maturation into infectious virions.

77 otein is incorporated into the capsid during DNA packaging, and previous studies of a null mutant hav

78 e number of theoretical studies have modeled DNA packaging, and the nature of the molecular dynamics

79 Central to DNA packaging are dynamic interactions among the packagi

80 particles in which expulsion of scaffold and DNA packaging are incomplete.

81 the terminase holoenzyme, DNA maturation and DNA packaging, are discussed.

82 rtal complexes in capsid assembly, including DNA packaging, as well as portal ring assembly and incor

83 A new phage T4 in vitro DNA packaging assay employed purified proheads, terminas

84 crystal structure of the portal protein and DNA packaging assays, these reconstructions indicate tha

85 Rather, the density of DNA packaging at all endopolyploid levels in the linc1 l

86 'portal vertex' and consists of a portal, a DNA packaging ATPase and other components.

87 and hydrolyzes ATP despite the fact that the DNA packaging ATPase site in the N terminus of gpA has b

88 dV protein IVa2 is presumed to function as a DNA packaging ATPase.

89 tein fold, an internal lipid membrane, and a DNA-packaging ATPase.

90 Unraveling the structure and assembly of the DNA packaging ATPases of the tailed double-stranded DNA

91 ort the complete atomic model of the headful DNA-packaging bacteriophage Sf6 at 2.9 A resolution dete

92 ee proteins are among six required for HSV-1 DNA packaging but dispensable for assembly of immature c

93 These cosB suppressors cause enhanced DNA packaging by 21-specific terminase and reduce packag

94 ata, in which electrostatic forces drive the DNA packaging by alternating between tensed and relaxed

95 ectly and specifically interferes with phage DNA packaging by blocking the phage terminase small subu

96 DNA packaging by double-stranded DNA bacteriophages and

97 DNA packaging by large DNA viruses such as the tailed ba

98 We go on to show how DNA packaging can induce spontaneous reorganization of t

99 similar volumes, consistent with comparable DNA packaging capacities.

100 variety of animal and human cells, has high DNA packaging capacity, and can conveniently be maintain

101 on dsDNA bacteriophages have revealed that a DNA packaging complex assembles at a special vertex call

102 nase cutting at cos, to generate the active, DNA packaging complex.

103 ter to initiate production of structural and DNA packaging components.

104 e single-turnover ATPase time course, and no DNA packaging, consistent with a critical role in ATP-co

105 nanoscale viral channel at the late stage of DNA packaging could be a consequence of Brownian movemen

106 We have analyzed our DNA packaging data according to an N-step sequential min

107 These proheads also showed no DNA packaging defect in vitro as compared with WT.

108 he mild DNA packaging defects, a severe post-DNA packaging defect.

109 ransferred to the restrictive temperature, a DNA-packaging defect was evident.

110 mutant enzymes have, in addition to the mild DNA packaging defects, a severe post-DNA packaging defec

111 K84A enzyme had similar virion assembly and DNA packaging defects.

112 the nucleosome, in addition to its role as a DNA packaging device, is a signaling module through whic

113 ith its central domain and extends its 5'/3' DNA-packaging domain for gp16 binding.

114 nition of the viral genome for initiation of DNA packaging during virus assembly.

115 he role of electrostatic repulsion on single DNA packaging dynamics in bacteriophage phi 29 via optic

116 DNase I protection assay indicated that the DNA packaging efficiency of the VLF-1(Y355F) virus const

117 The DNA-packaging efficiency with dimeric pRNA was more seri

118 ested the existence of a bacteriophage-like, DNA-packaging/ejecting portal complex in herpesviruses c

119 ng complexes and the dynamics of TerL during DNA packaging, endonuclease regulation, and motor mechan

120 The DNA packaging energy budget, i.e. DNA packaged/ATP hydro

121 Transcription factor A (TFAM) functions as a DNA packaging factor in mammalian mitochondria.

122 nd the high mobility group box transcription/DNA-packaging factor, h-mtTFA/TFAM.

123 porting the suggested role of this pocket in DNA packaging for the Dependoparvovirus The structural c

124 However, recent single-particle DNA packaging force measurements have suggested that inc

125 dy we show that the L procapsid assembly and DNA packaging genes, which encode terminase, portal, sca

126 Even though DNA packaging has been studied extensively, currently no

127 of a 30-nm fiber as a basic folding unit for DNA packaging has remained a topic of active discussion.

128 ure composed of modules for DNA replication, DNA packaging, head and tail morphogenesis, and lysis.

129 tant post-translational modifications to the DNA packaging histones on the normal genome and the PSR

130 In the final stages of DNA packaging, however, the internal genome pressure dra

131 on and propose a structural basis for random DNA packaging.IMPORTANCE Random transfer of any and all

132 bserved in recent single-molecule studies of DNA packaging in bacteriophage lambda.

133 Here, we present measurements of viral DNA packaging in bacteriophage phi29 using an improved o

134 chanics models provide a detailed picture of DNA packaging in bacteriophage, revealing how conformati

135 Double-stranded DNA packaging in bacteriophages is driven by one of the

136 s inherent in the biological significance of DNA packaging in cell nuclei, as well as for gene therap

137 lled nucleosomes, are the building blocks of DNA packaging in eukaryotic cells.

138 ht DNA bending is biologically important for DNA packaging in eukaryotic chromatin and tight DNA loop

139 lled protamines mediate the near crystalline DNA packaging in most vertebrate sperm cells.

140 nzyme complexes, which facilitate ATP-driven DNA packaging in phages and in many eukaryotic viruses,

141 tors were restarted to observe the motion of DNA packaging in real time.

142 Nase protection assay commonly used to study DNA packaging in several bacteriophage systems, such tha

143 functions of disulfide linkages for in vivo DNA packaging in sperm chromatin.

144 s have biological implications for protamine-DNA packaging in sperm heads.

145 common mechanism for the initiation step of DNA packaging in tailed double-stranded DNA bacterial vi

146 DNA packaging in the bacteriophage phi29 involves a mole

147 nown, it has been proposed to facilitate the DNA packaging in the prokaryotic nucleoid and/or to prom

148 the CCA bulge in this helix is essential for DNA packaging in vitro.

149 t structural compaction which may facilitate DNA packaging in vivo.

150 T3 phage capsids (ipDNA-capsids) produced by DNA packaging in vivo; some ipDNA has quantized lengths,

151 Evidence that in vivo bacteriophage T3 DNA packaging includes capsid hyper-expansion that is tr

152 chemical information, we propose a model for DNA packaging initiation.

153 three-dimensional pseudo-atomic model for a DNA-packaging initiation complex assembled from the term

154 Subsequent DNA packaging instigates bending of MCP A domain loops o

155 erlying the forces and pressures involved in DNA packaging into bacteriophage capsids became the focu

156 nomic DNA and no appreciable change in viral DNA packaging into capsids.

157 ed by SaPIbov1 gene 12, which prevents phage DNA packaging into small capsids, and which is also prim

158 alysis of the proteome of the 9NA virion and DNA packaging into the 9NA phage head.

159 e not required for procapsid assembly or for DNA packaging into the structure, but that proteolysis i

160 enzyme thought to provide energy that drives DNA packaging into the virion during infection.

161 statements regarding my recent letter about DNA packaging into viral capsids.

162 e this assembly step is unique to the virus, DNA packaging is a novel target for the development of a

163 Adenovirus type 5 (Ad5) DNA packaging is initiated in a polar fashion from the l

164 The resulting DNA packaging is known to interfere with assembly of the

165 se, gp16, is also essential, but its role in DNA packaging is poorly understood.

166 A key event in DNA packaging is recognition of the viral DNA among othe

167 Finding that DNA packaging is unimpeded in proheads containing the fu

168 the terminal stage of viral DNA development, DNA packaging, is poorly understood.

169 To elucidate the mechanism of phi29 DNA packaging, it is critical to confirm whether pRNA bi

170 e involved in virion structure and assembly, DNA packaging, lysis, and DNA metabolism.

171 The bacteriophage T4 DNA packaging machine consists of a molecular motor asse

172 initiation and assembly of a supramolecular DNA packaging machine on the viral concatemer.

173 y filled with the viral genome by means of a DNA packaging machine situated at a special fivefold ver

174 ly steps reflecting formation of the active, DNA packaging machine.

175 nnector, which may potentiate the release of DNA-packaging machine components, creating a binding sit

176 A model for the assembly of the T4 DNA-packaging machine is presented.

177 structure of the ATPase component in a phage DNA-packaging machine.

178 nizations and sequence conservation in their DNA packaging machinery and maturational proteases.

179 Thus the phi29 DNA packaging machinery tolerated nicks, but stopped at

180 The small terminase (TerS) component of this DNA-packaging machinery acts as a molecular matchmaker t

181 in interacting with tegument proteins or the DNA-packaging machinery at the portal vertex.

182 sophisticated strategies to hijack the phage DNA-packaging machinery for their own transfer.

183 ahedral procapsid, which then expands during DNA packaging (maturation).

184 Several models for the DNA packaging mechanism have been proposed, which attrib

185 s binding properties implicate a role in the DNA packaging mechanism.

186 al rotation requirement of some hypothetical DNA packaging mechanisms.

187 rocess but also can be useful in deciphering DNA-packaging mechanisms for other viruses belonging to

188 Indeed, studies on viral DNA packaging might lead to the development of new antiv

189 s, gp17, and DNA are actively engaged in the DNA packaging mode.

190 symmetry of the portal protein has prompted DNA packaging models in which ATP-driven portal protein

191 Recent DNA-packaging models proposed that the 5-fold procapsid

192 hat the packaging RNA of bacteriophage phi29 DNA packaging motor can be assembled from 3-6 pieces of

193 kaging RNA (pRNA) of the bacteriophage phi29 DNA packaging motor has been developed into a nanodelive

194 ing about possible applications of the viral DNA packaging motor in nanotechnology and medical applic

195 e of such a ring ATPase, the double-stranded-DNA packaging motor in the bacteriophage phi29.

196 plication of the pRNA-3WJ motif of the phi29 DNA packaging motor into fusion RNA with controllable an

197 is an essential component of the ATP-driven DNA packaging motor of bacteriophage o29.

198 (pRNA), which is a central component of the DNA packaging motor of bacteriophage phi29.

199 sequential action of the ATPase ring in the DNA packaging motor of bacteriophage varphi29 is regulat

200 The DNA packaging motor of the Bacillus subtilis bacteriopha

201 The DNA packaging motor of the bacteriophage varphi29, compr

202 We report the crystal structure of the DNA packaging motor protein, gene product 17 (gp17), in

203 The bacteriophage o29 DNA packaging motor that assembles on the precursor caps

204 acteriophage inside a bacterial host cell, a DNA packaging motor transports the viral DNA into the pr

205 n (3WJ) of the pRNA from bacteriophage phi29 DNA packaging motor were examined previously for ocular

206 RNA polymerase (RNAP)(6), gyrase(2), a viral DNA packaging motor(7) and DNA recombination enzymes(8).

207 This vertex is the site of assembly of the DNA packaging motor, and the connector has a central cha

208 ucleotide prohead RNA that is part of the 29 DNA packaging motor, at constant value of rf occurs with

209 sential component in the phi29 bacteriophage DNA packaging motor, the strongest biomolecular motor kn

210 system is the Bacillus subtilis phage phi 29 DNA packaging motor, which generates large forces to com

211 NA into its preformed procapsid requires the DNA packaging motor, which is the strongest known biolog

212 d operation of the powerful bacteriophage 29 DNA packaging motor.

213 f the self-assembling varphi29 bacteriophage DNA packaging motor.

214 t hexameric, pRNA ring in the context of the DNA packaging motor.

215 that forms the central channel of the phage DNA packaging motor.

216 to pRNA binding during assembly of an active DNA packaging motor.

217 ation is driven by an ATP hydrolysis-powered DNA packaging motor.

218 stalk domain are involved in assembling the DNA packaging motor.

219 The pRNA of bacteriophage phi29 DNA-packaging motor also forms a ring, but whether the p

220 to the assembly and function of a terminase DNA-packaging motor are discussed.

221 The central hub of the bacteriophage phi29 DNA-packaging motor contains a 3.6-nm channel for dsDNA

222 Here we report the structures of the DNA-packaging motor gp2 of bacteriophage Sf6, which reve

223 ults with respect to the assembly of a viral DNA-packaging motor is discussed.

224 Bacteriophage phi29 DNA-packaging motor is geared by a packaging RNA (pRNA)

225 We have recently found that the DNA-packaging motor of bacteriophage phi29 uses a third

226 Tailed bacteriophages use a DNA-packaging motor to encapsulate their genome during v

227 third type of biomotor, including the viral DNA-packaging motor, beside the bacterial DNA translocas

228 The bacteriophage phi29 DNA-packaging motor, which allows double-stranded DNA to

229 of DNA translocation and cleavage in a viral DNA-packaging motor, which may be achieved via linker-do

230 locases such as cellular helicases and viral DNA packaging motors (terminases).

231 Viral DNA packaging motors are among the most powerful molecul

232 has long been popularly believed that viral DNA packaging motors are rotation motors.

233 e findings support the hypothesis that viral DNA packaging motors contain an adenine-binding motif th

234 Bacteriophage DNA packaging motors translocate their genomic DNA into

235 rce generation is a common property of viral DNA packaging motors.

236 ility also occur in the study of other viral DNA-packaging motors.

237 velopment of antiviral drugs targeting viral DNA-packaging motors.

238 In the Phi29 bacteriophage, the DNA packaging nanomotor packs its double-stranded DNA ge

239 on of the channel of the bacteriophage phi29 DNA-packaging nanomotor and its subsequent incorporation

240 As a result of dense DNA packaging, NFs are resistant to nuclease degradation

241 uded a 308-bp deletion (20%) in the prophage DNA packaging Nu1 and a 730-bp insertion of the green fl

242 Termination of the DNA packaging occurs through pressure-dependent correlat

243 ese data support a model in which adenovirus DNA packaging occurs via the formation of a IVa2 multipr

244 e the major virion proteins and examined the DNA packaging of the prototypic member, phage 9NA.

245 ATP-driven DNA packaging of the T4 capsid results in the loading of

246 studies were undertaken to determine whether DNA packaging or capsid assembly affected the pU(L)17/pU

247 branching, or damage repair concomitant with DNA packaging or cleavage.

248 on of a function of this protein relevant to DNA packaging other than its interaction with other term

249 We also mapped phage 9 g DNA packaging (pac) site containing two 21-bp direct rep

250 al DNA substrate, the very first step in the DNA packaging pathway, and stimulates the ATPase and pac

251 proteins suggest that they may have roles in DNA packaging, penton formation, and protein-protein int

252 ange determination of CATC attachment during DNA packaging prior to pleomorphic tegumentation/envelop

253 d that the portal is required throughout the DNA packaging process, with different domains participat

254 tial function during the final stages of the DNA packaging process.

255 assembly models and an understanding of the DNA packaging process.

256 e of herpesvirus-specific adaptations in the DNA-packaging process.

257 tely 50 atmospheres as a result of the phage DNA-packaging process.

258 ously reported to interact with the putative DNA packaging protein A32 in a yeast two-hybrid screen.

259 uting the coding region for the L1 52/55 kDa DNA packaging protein from HAdV-16.

260 genetic analysis indicated that L1 52/55-kDa DNA packaging protein may be associated with the higher

261 or capsid proteins VP5 and VP23, but not the DNA packaging protein pU(L)15.

262 bacteriophages encode a heterodimeric viral DNA packaging protein, called terminase.

263 53, each of these prophages encodes head and DNA packaging proteins related to the siphophage lambda

264 Since the presence and modification of these DNA-packaging proteins provide a scaffold for docking of

265 Of the three capsid-associated DNA-packaging proteins, UL17, UL25, and UL6, only UL17 a

266 mechanism of gpFI chaperone activity in the DNA packaging reaction of phage lambda.

267 termed the terminase, associates during the DNA packaging reaction.

268 t it reports directly on the kinetics of the DNA packaging reaction.

269 DNA bending is critical for DNA packaging, recognition, and repair, and occurs towar

270 However, this DNA packaging reduces accessibility of enzymes that act

271 for nuclear/cytoplasmic proteins involved in DNA packaging, replication, and protein synthesis were d

272 DNA packaging requires an ATPase catalytic site situated

273 -base pRNA supported the correct polarity of DNA packaging, selectively packaging the DNA left end.

274 ion could be further enhanced by using viral DNA packaging sequences (inverted terminal repeats [ITRs

275 terminally redundant and that initiation of DNA packaging series occurs across an approximately 1-kb

276 The DNA-packaging specificities of phages lambda and 21 depe

277 TerS, however, determines DNA packaging specificity but not the choice of large ve

278 es and many tailed bacteriophages includes a DNA packaging step where the viral genome is inserted in

279 f residues is incompatible with the observed DNA packaging step-size of approximately 2.5 bp, such th

280 by similarities in their capsid assembly and DNA packaging strategies.

281 ludes modules for lysogeny, DNA replication, DNA packaging, structural proteins, and host cell lysis.

282 tex is critical for driving capsid assembly, DNA packaging, tail attachment, and genome ejection.

283 ase subunit), UL23 (thymidine kinase), UL25 (DNA packaging tegument protein), and UL52 (helicase-prim

284 roteins, including the ATPase subunit of the DNA-packaging terminase, the only protein with previousl

285 Many dsDNA viruses encode DNA-packaging terminases, each containing a nuclease dom

286 - and C-terminal domains, inhibited in vitro DNA packaging that is catalyzed by full-length gp17.

287 Upon DNA packaging, the capsid lattice expands and becomes a

288 results suggest that, in the early stage of DNA packaging, the dsDNA genome is randomly distributed

289 At the late stage of DNA packaging, the negatively charged genome is increasi

290 Mapping of nucleosomes, the basic DNA packaging unit in eukaryotes, is fundamental for und

291 DNA packaging using restriction digests of varphi29 DNA

292 te of ATP hydrolysis, 2.5- to 4.5-fold lower DNA packaging velocity, and required an activator protei

293 a requirement of the viral IVa2 protein for DNA packaging via a direct interaction with packaging se

294 The C18C19A20 bulge that is essential for DNA packaging was found to be dispensable for gp16 bindi

295 in and test whether it is indeed involved in DNA packaging, we constructed a recombinant vaccinia vir

296 s of the CVSC proteins in virus assembly and DNA packaging, we isolated a number of recombinant virus

297 ction/hyper-expansion cycles that accelerate DNA packaging when packaging is slowed by increase in th

298 nces, a capsid expansion process accompanies DNA packaging, which significantly increases the volume

299 ulate gp17-ATPase, an activity essential for DNA packaging, while conversion back into oligomeric for

300 e observed, may allow phage T4 to coordinate DNA packaging with other ongoing processes, including vi