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1 by generating chimeras with hASNase1 through DNA shuffling.
2 y from a cellulase library created by family DNA shuffling.
3 d on in vitro recombination methods, such as DNA shuffling.
4 dely used in vitro DNA recombination method, DNA shuffling.
5 dition to library generation methods such as DNA shuffling.
6 to analyze chimeric DNA libraries created by DNA shuffling.
7 ent protein was subjected to mutagenesis and DNA shuffling.
8 ation for the creation of hybrid enzymes and DNA shuffling.
9 crossover statistics and draws contrast with DNA shuffling.
10 eling a specific DNA recombination protocol, DNA shuffling.
11 roteins and enzymes have been improved using DNA shuffling.
12 ropic murine leukemia virus (MLV) strains by DNA shuffling.
13 qual to the activity of the hybrids found by DNA shuffling.
14          This mutagenesis approach is called DNA shuffling.
15 hniques such as directed evolution including DNA shuffling, a great number of BBB-crossing AAVs have
16 r multiple rounds of cycling mutagenesis and DNA shuffling, a more efficient nuclease variant (Sharke
17                            After 3 cycles of DNA shuffling, a mutant was obtained with a whole cell f
18 iscipline was galvanized by the invention of DNA shuffling, a procedure that randomly recombines poin
19                              Seven rounds of DNA shuffling and colony screening on chromogenic fucose
20 other into extinction (clonal interference); DNA shuffling and combinatorial cassette mutagenesis led
21          We envision that the combination of DNA shuffling and high throughput screening will be a po
22                              Eight rounds of DNA shuffling and in vivo selection followed by a backcr
23 erent PRRSV strains were molecularly bred by DNA shuffling and iteration of the process, and the shuf
24            Our results suggest that combined DNA shuffling and mutagenesis of libraries of Flp varian
25                           Repeated rounds of DNA shuffling and oligonucleotide-directed mutagenesis f
26                                              DNA shuffling and other in vitro recombination strategie
27                           More specifically, DNA shuffling and other methods of genetic recombination
28  in vivo capsid evolution through sequential DNA shuffling and peptide library screening in a NF1 xen
29 een altered by stochastic mutations based on DNA shuffling and rationally tailored by structure-based
30 utant, TG-15, was subjected to iterations of DNA shuffling and screened for enzyme variants with up-r
31                    Here we use two cycles of DNA shuffling and screening in Escherichia coli to obtai
32 oped a more potent version of IL-12 by using DNA shuffling and screening to improve its expression in
33  among 32 clones isolated in three rounds of DNA shuffling and screening were mapped to the active si
34 s in specificity and activity by reiterative DNA shuffling and screening, even for an enzyme of 109 k
35 e substrate) were evolved in seven rounds of DNA shuffling and screening.
36 esus monkey, baboon, cat, cow, and rabbit by DNA shuffling and screening.
37 re recombined in two cycles of high-fidelity DNA shuffling and screening.
38            Further rounds of evolution using DNA shuffling and staggered extension process (StEP) res
39 ies, requires fewer PCR cycles than in vitro DNA shuffling and, unlike site-specific recombination me
40 tion to existing DNA recombination methods ('DNA shuffling') and should be particularly useful for re
41 f enzymes resulting from random mutagenesis, DNA shuffling, and combinatorial saturation mutagenesis
42 on of error-prone polymerase chain reaction, DNA shuffling, and multiple-site-directed mutagenesis to
43 was isolated after three rounds of mutation, DNA shuffling, and screening.
44 nzymes, including site-directed mutagenesis, DNA shuffling, and site-saturation mutagenesis, among ot
45                                 We evaluated DNA shuffling applied to the three complementarity-deter
46       Our CODNS (cross-over optimization for DNA shuffling) approach employs polynomial-time dynamic
47         We describe a computational model of DNA shuffling based on the thermodynamics and kinetics o
48 eric Pgp with an altered resistance profile, DNA shuffling between the homologous but not identical d
49                                              DNA shuffling can be combined with screening of a modera
50                       Molecular breeding via DNA shuffling can direct the evolution of viruses with d
51                      These results show that DNA shuffling can improve the function of pathways by co
52  of improved variants from the two rounds of DNA shuffling confirmed important features of the recomb
53  High fidelity in vitro gene recombination ("DNA shuffling") coupled with sequence analysis of a smal
54 or assessing the generation of crossovers in DNA shuffling experiments.
55                                              DNA shuffling facilitated the evolution of a human immun
56 odon usage and performed recursive cycles of DNA shuffling followed by screening for the brightest E.
57 s resource-intensive and more effective than DNA shuffling for this particular evolutionary pathway.
58  An efficient beta-fucosidase was evolved by DNA shuffling from the Escherichia coli lacZ beta-galact
59                                              DNA shuffling generates combinatorial libraries of chime
60                                        While DNA shuffling has been applied quite successfully, it is
61 m mutagenesis, site-directed mutagenesis and DNA shuffling, have been widely used to generate variant
62                                              DNA shuffling identified the beneficial NagAc mutation G
63                         Eleven iterations of DNA shuffling improved enzyme efficiency by nearly four
64                                 Furthermore, DNA shuffling improved the level of expression and homog
65     These studies demonstrate the utility of DNA shuffling in breeding viral strains with improved ch
66 c resistance operon has been accomplished by DNA shuffling, involving multiple rounds of in vitro rec
67                                              DNA shuffling is a powerful process for directed evoluti
68                                              DNA shuffling is a practical process for directed molecu
69 presence of synergistic reassembly in family DNA shuffling is obtained.
70                        At a molecular level, DNA shuffling mimics, yet accelerates, evolutionary proc
71                      The in vitro process of DNA shuffling (molecular breeding) mimics this mechanism
72 DNA sequences for recombination, we combined DNA shuffling mutagenesis and a forward selection strate
73                                              DNA shuffling of a family of over 20 human interferon-al
74        Four rounds of random mutagenesis and DNA shuffling of Drosophila melanogaster 2'-deoxynucleos
75 th in vitro and in vivo, indicating that the DNA shuffling of GP4 and M genes did not significantly i
76                                              DNA shuffling of mutations from the various screens and
77                                              DNA shuffling of NDO nagAcAd also generated the NagAc va
78                          Error-prone PCR and DNA shuffling of the EK(L) gene, T7 promoter, lac operon
79 this study we molecularly bred PRRSV through DNA shuffling of the GP4 and M genes, separately, from s
80                     The results suggest that DNA shuffling of the GP4 or M genes from different paren
81                 Here, random mutagenesis and DNA shuffling of the single-chain variable fragment of t
82 n of the virus was achieved in this study by DNA shuffling of the viral envelope genes from multiple
83 this study reveals a unique approach through DNA shuffling of viral envelope genes to attenuate a pos
84 ified as beneficial by random mutagenesis or DNA shuffling or seen in any of the naturally occurring
85  of hGSTT1-1 constructed by error-prone PCR, DNA shuffling, or saturation mutagenesis were screened f
86 etween phenotypes and variation generated by DNA shuffling paralleled natural variation observed betw
87                         Three aspects of the DNA shuffling procedure are modeled: the fragment size d
88 ed chimeric sections are then subjected to a DNA shuffling process generating an enhanced crossover S
89 ults led to several unique insights into the DNA shuffling process.
90                     Two subsequent rounds of DNA shuffling produced additional clones that showed fur
91 t diversity targets are investigated for the DNA shuffling protocol to showcase the utility of the eC
92                                A convenient 'DNA shuffling' protocol for random recombination of homo
93                           We previously used DNA shuffling (sexual recombination) and a histochemical
94                  Our less biased approach to DNA shuffling should be useful for the engineering of a
95 ned sequence; (ii) minimizing bias in family DNA shuffling so that each of the parental sequence pair
96 his artificial family is then subjected to a DNA-shuffling step to augment the number of crossovers.
97         These results were validated using a DNA shuffling strategy and through quantum mechanical/mo
98                                              DNA shuffling technology has been significantly enhanced
99 enesis of cloned genes by error-prone PCR or DNA shuffling that eliminates the need for post-amplific
100  the protein engineer with a new approach to DNA shuffling that supports substantially more diverse p
101         We compared the ability of ITCHY and DNA shuffling to create interspecies fusion libraries be
102                           We previously used DNA shuffling to direct the evolution of Escherichia col
103                                      We used DNA shuffling to explore and confirm the hypothesis that
104 nts efficient methods to extend the scope of DNA shuffling to handle significantly more diverse paren
105                                        Using DNA shuffling to introduce functional sequence variabili
106 te spectrum, we used rounds of selection and DNA shuffling to obtain GroEL/S variants that dramatical
107 ed iterative site-saturation mutagenesis and DNA shuffling to screen precise gene-variant yeast displ
108 oduce a method of in vitro recombination or "DNA shuffling" to generate libraries of evolved enzymes.
109                                              DNA shuffling was used as a combinatorial in vitro genet
110 tion mutagenesis and in vitro recombination (DNA shuffling) was used to generate mutant libraries, wh
111  combinatorial random mutagenesis technique (DNA shuffling), we have isolated an IE(ME)-specific hype
112 xplore whether there might be limits to such DNA shuffling, we have mapped the termini of mitochondri
113                                        Using DNA shuffling, we isolated specific amino acids combinat
114 antage of multi-parental crossing allowed by DNA shuffling with the recombination of entire genomes n
115 nt cln2-KAEA, but additional mutagenesis and DNA shuffling yielded multiply mutant CDC28-BYC alleles

 
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