ライフサイエンスコーパス: DNAzyme

1 g of temporarily inactivated deoxyribozymes (DNAzymes).

2 e demonstrate for the 10-23 RNA endonuclease DNAzyme.

3 Tm7 is an Er(3+)-dependent RNA-cleaving DNAzyme.

4 ining aniline, H2O2, and a G-qudraplex-hemin DNAzyme.

5 and consequently the activity of peroxidase-DNAzyme.

6 ream activator after cleavage by an upstream DNAzyme.

7 triggered activation of the Mg(2+)-dependent DNAzyme.

8 lly relevant uranyl-binding sites in the 39E DNAzyme.

9 of nanowires consisting of the HRP-mimicking DNAzyme.

10 deoxyuridine by UNG, however, activated the DNAzyme.

11 me-driven activation of the Mg(2+)-dependent DNAzyme.

12 t role in the catalytic function of the 8-17 DNAzyme.

13 IL13 than cells from mice given the control DNAzyme.

14 nger RNA, compared with mice given a control DNAzyme.

15 lent transition metal dependent RNA-cleaving DNAzyme.

16 labeling a fluorophore and a quencher on the DNAzyme.

17 ine-Hg(2+) interactions and Hg(2+)-activated DNAzymes.

18 logy, rolling circle amplification (RCA) and DNAzymes.

19 when complexed with hemin become functional DNAzymes.

20 that improve the affinity and specificity of DNAzymes.

21 dependent deactivation and activation of the DNAzymes.

22 shift in the CD spectra of the G-quadruplex DNAzymes.

23 ilt high hopes for designing novel catalytic DNAzymes.

24 ied by aptamers, riboswitches, and ribozymes/DNAzymes.

25 s, aptamers, peptides, protein scaffolds and DNazymes.

26 incorporating the PS modification to another DNAzyme, a sensor array was prepared to detect each meta

27 The present study demonstrates DNAzyme activation in the presence of metal ions (Pb(2+)

28 rm a near bioorthogonal pair with I-SceI for DNAzyme activation with minimal effect on living cells.

29 mer complexes remaining on the surface after DNAzyme activity can be greatly enhanced (down to one th

30 tivation method is presented for controlling DNAzyme activity in living cells.

31 Irradiation at 365 nm restored DNAzyme activity, thus allowing the temporal control ove

32 8-17 DNAzyme adopts a V-shape fold, and the Pb(2+) cofactor i

33 The combination of PNA, RCA, and DNAzymes allows for sequence-specific and highly sensiti

34 Finally, the hemin/G-quadruplex DNAzyme/Amplex Red system was used to follow the activit

35 In addition, the cumulative nature of the DNAzyme-amplified signal generation process produced a d

36 as substrate for the DNAzyme, PCR-amplified DNAzyme-amps generated in the presence of MCR-2 gene wer

37 DNAzyme into the primer pair, PCR-amplified DNAzyme-amps were generated, tested, and validated on qP

38 vage activity of DNAzyme-extended amplicons (DNAzyme-amps) is established, followed by optimization o

39 interaction between the active-conformation DNAzyme and a small molecule dye, N-methylmesoporphyrin

40 Colonic distribution of labeled DNAzyme and inflammation were monitored by in vivo imagi

41 NA chains consisting of the Mg(2+)-dependent DNAzyme and sequences that are complementary to the loop

42 sly reported the photochemical activation of DNAzymes and antisense agents through the preparation of

43 estigating the photochemical deactivation of DNAzymes and antisense agents.

44 ucleotides prohibits the formation of active DNAzymes and eliminates the release of the respective dy

45 ng a library of predesigned Mg(2+)-dependent DNAzymes and their respective substrates.

46 This is a new DNAzyme, and a catalytic beacon sensor is designed by at

47 mplementary sequence to the Mg(2+)-dependent DNAzyme, and a sequence identical to the loop region of

48 Using RNase digestion, DNAzyme, and RNA mobility shift assays, we demonstrate t

49 Our results showed that CRET-based DNAzyme-aptamer biosensing enabled specific OTA analysis

50 We have designed a single-stranded DNAzyme-aptamer sensor for homogeneous target molecular

51 ytic activities as compared to the separated DNAzyme/aptamer units, and the most active nucleoapzyme

52 e targets that are difficult to recognize by DNAzymes, aptamers, or antibodies, and without the need

53 uplex nanostructure and the Pb(2+)-dependent DNAzyme are implemented to develop sensitive surface pla

54 DNAzymes are a promising platform for metal ion detectio

55 ence of Mg(2+) or Zn(2+) ions the respective DNAzymes are activated, leading to the specific cleavage

56 DNAzymes are DNA-based catalysts; they typically recruit

57 DNAzymes are enzymatically active deoxyoligonucleotides

58 DNAzymes are known to bind metal ions specifically to ca

59 two decades ago, the metal-binding sites in DNAzymes are not fully understood.

60 While no Tl(3+)-dependent DNAzymes are obtained, a DNA oligonucleotide containing

61 RNA-cleaving DNAzymes are the catalytic DNAs discovered the earliest,

62 DNA strands with catalytic activity (DNAzymes) are an attractive alternative, enabling ration

63 Deoxyribozymes (DNAzymes) are single-stranded DNA that catalyze nucleic

64 RNA-cleaving deoxyribozymes (DNAzymes) are synthetic single-stranded DNA-based cataly

65 alyzing DNA by the Zn(2+)-dependent ligation DNAzyme as amplifying biocatalyst is presented.

66 g the horseradish peroxidase (HRP)-mimicking DNAzyme as an amplifying label.

67 tch produces an active nucleic acid-cleaving DNAzyme as an output and this allows the switches to be

68 uplex horseradish peroxidase (HRP)-mimicking DNAzyme as catalytic labels that provide colorimetric or

69 as supporting matrix and hemin/G-quadruplex DNAzyme as signal amplifier for determination of hepatit

70 As uranyl is the cofactor of the 39E DNAzyme as well as the probe, specific uranyl binding ha

71 cific recognition elements and peroxidase or DNAzymes as chemiluminescence reporters.

72 onment, making it the first demonstration of DNAzymes as intracellular metal ion sensors.

73 the peroxidase-mimicking DNAzyme (peroxidase-DNAzyme) as general and inexpensive platform for develop

74 resulting nanostructures bear split parts of DNAzyme at each end of the four arms which, in the prese

75 on recent crystallographic data, on the 8-17 DNAzyme at four states along the reaction pathway to det

76 tionalization of a therapeutic mRNA-cleaving DNAzyme at the particle's surface.

77 ovel therapeutic inhaled GATA3 mRNA-specific DNAzyme attenuated early- and late-phase allergic respon

78 We demonstrate that this DNAzyme-AuNP probe can readily enter cells and can serve

79 ying enzymes and expand the analyte range of DNAzyme-based biosensors.

80 Henceforth, this DNAzyme-based fiber optic PCR assay provides a universal

81 In addition, the DNAzyme-based FO-PCR assay was able to discriminate betw

82 While DNAzyme-based metal sensors have found many applications

83 Furthermore, we demonstrate that this DNAzyme-based sensor can readily enter cells with the ai

84 ether, these results demonstrate that such a DNAzyme-based sensor provides a promising platform for d

85 DNAzyme-based sensors are highly attractive for their ex

86 platform for metal ion detection, and a few DNAzyme-based sensors have been reported to detect metal

87 The IC 3D integrates real-time, DNAzyme-based sensors, droplet microencapsulation and a

88 r range of analytes to take advantage of the DNAzyme-based signal amplification for more sensitive de

89 A DNAzyme beacon was engineered detecting down to 1.7 nM C

90 affinity interaction between the peroxidase-DNAzyme bearing hairpin sequence and the analyte (DNA or

91 wnian dynamics simulations, we find that the DNAzyme bends its substrate away from the cleavage point

92 The 5'-end of the DNAzyme binds the substrate DNA via Watson-Crick bonding

93 rovides a new route to obtain metal-specific DNAzymes by atomic replacement and also offers important

94 lar engineering to improve the properties of DNAzymes by designing a unimolecular probe for lead ion

95 detecting metal ions, as many metal-specific DNAzymes can be obtained using in vitro selection.

96 Since a wide variety of metal-specific DNAzymes can be obtained, this method can likely be appl

97 The SCS can be activated by various upstream DNAzymes, can be coupled to DNA strand-displacement devi

98 ge of DNAzyme moieties from miRNA-hybridized DNAzyme-capped capture probes (DZ-CPs) from magnetic bea

99 mechanistic insights into metal binding and DNAzyme catalysis.

100 l understanding of multivalent ion dependent DNAzyme catalysis.

101 exposing the reactive site and buckling the DNAzyme catalytic core.

102 study describes the novel hemin/G-quadruplex DNAzyme-catalyzed aerobic oxidation of thiols to disulfi

103 Consequently, the CRET occurred between a DNAzyme-catalyzed chemiluminescence reaction and the que

104 The DNAzyme-catalyzed cleavage of a fluorophore/quencher-mod

105 hrough the fluorescence enhancement from the DNAzyme-catalyzed cleavage of DNA substrates labeled by

106 metric detection was carried out through the DNAzyme-catalyzed oxidation of 3,3',5,5'-tetramethylbenz

107 steine, glutathione) using the H2O2-mediated DNAzyme-catalyzed oxidation of Amplex Red to the resoruf

108 The mechanism of the reaction involves the DNAzyme-catalyzed oxidation of thiols to disulfides and

109 The DNAzyme catalyzes the polymerization of aniline and the

110 tidine C13 in the catalytic core of the same DNAzyme caused significant decrease of the activity.

111 An RNA-cleaving DNAzyme (Ce13d) was recently reported to be active with

112 Screening phase-generated DNAzymes characterized by either good catalytic activity

113 thrombin-aptamer complex was found to block DNAzyme cleavage activity both in solution and in an ssD

114 arrays are explored with DNA hybridization, DNAzyme cleavage, and nuclease digestion experiments.

115 ligated product, and the resulting assembled DNAzyme cleaves a fluorophore/quencher-modified substrat

116 ion and in the presence of Na(+) , the NaA43 DNAzyme cleaves its substrate strand and releases a prod

117 In the presence of uranyl, the DNAzyme cleaves the fluorophore-labeled substrate strand

118 resence of Pb(2+) ions, the Pb(2+)-dependent DNAzyme cleaves the substrate, leading to the separation

119 rapid detection of ascorbic acid (AA) with a DNAzyme cleaving its DNA substrate in the presence of Cu

120 nto the system, and this hybridizes with the DNAzyme components and releases the ligated product for

121 f the CDNs is based on the activities of the DNAzymes conjugated to the constituents, the fluorescenc

122 diagnostics, we have developed a peroxidase DNAzyme construct that can be used as a chromogenic func

123 DNAzyme contains a loop forming a complex with Cu(2+) io

124 Here, we report three catalytic form 8-17 DNAzyme crystal structures.

125 ed controlled activation of the sensor after DNAzyme delivery into cells.

126 (DNAzyme-DPs) brought the target SNP and the DNAzyme-DPs onto the magnetic beads.

127 robes containing multiple DNAzyme sequences (DNAzyme-DPs) brought the target SNP and the DNAzyme-DPs

128 r presented by the Zn(2+)-dependent ligation DNAzyme-driven activation of the Mg(2+)-dependent DNAzym

129 First, the surface cleavage activity of DNAzyme-extended amplicons (DNAzyme-amps) is established

130 These 6MI point mutant DNAzymes fall into three distinct functional classes, wh

131 endogenous and bioorthogonal activation of a DNAzyme fluorescent sensor containing an 18-base pair re

132 In addition to RNA transcription, DNAzyme footprinting can be coupled to a wide variety of

133 r detecting protein-aptamer complexation as "DNAzyme footprinting" in analogy to the process of DNase

134 s I-SceI bioorthogonally activates the 10-23 DNAzyme for imaging of Mg(2+) in HeLa cells.

135 ral control over the sensing activity of the DNAzyme for metal ions.

136 e same strategy was also applied to the GR-5 DNAzyme for the detection of Pb(II), thus demonstrating

137 icient, visible light-harnessing, photolyase DNAzymes for either the prophylaxis or therapy of UV dam

138 as a one-step assay, driven by PCR-amplified DNAzymes, for FO-SPR-based sensitive and specific detect

139 concentration of HRP-mimicking G-quardruplex DNAzyme formed from the binding interaction between hemi

140 DP), the hybridization of which prevents the DNAzyme from being active.

141 A sequences and structures such as aptamers, DNAzymes, G-quadruplexes, and i-motifs can be readily pr

142 FNAs mainly include DNAzymes, G-quadruplexes, and mismatched base pairs and

143 e two "genes", e.g., the histidine-dependent DNAzyme g1 and the Zn(2+)-ion-dependent DNAzyme g2.

144 dent DNAzyme g1 and the Zn(2+)-ion-dependent DNAzyme g2.

145 on of a polymerization/nicking machinery and DNAzyme generation path leads to an improved analysis of

146 significant promise, cellular sensing using DNAzymes has however been difficult, mainly because of t

147 lencing for disease treatments, RNA-cleaving DNAzymes have been extensively studied; however, the mec

148 DNAzymes have been previously used to detect divalent me

149 DNAzymes have been widely explored owing to their excell

150 DNAzymes have enjoyed success as metal ion sensors outsi

151 DNAzymes have shown great promise as a general platform

152 A sequences (deoxyribozymes, DNA enzymes, or DNAzymes) have been identified by in vitro selection for

153 Although deoxyribozymes (DNAzymes) have been widely used as biosensors for the de

154 ation is unfavored and where the noncovalent DNAzyme-hemin complex has no activity.

155 The DNAzyme hgd40 inhibited expression of GATA3 messenger RN

156 outs); some mice were given a GATA3-specific DNAzyme (hgd40) or a control DNAzyme via intrarectal adm

157 aptamers that employs the inhibition of the DNAzyme hydrolysis of aptamer monolayers is described.

158 the oxidative catalytic activity of a split DNAzyme in a highly controllable manner.

159 -fourths and one-fourth of the HRP-mimicking DNAzyme in caged, inactive configurations are used as fu

160 by 2'-O-nitrobenzyl adenosine, rendering the DNAzyme inactive and thus allowing its delivery into cel

161 merging role of adaptive immunity underlying DNAzyme inhibition of cancer growth.

162 c determination system based on G-quadruplex DNAzyme integrated with a smartphone was developed to qu

163 0.1 min(-1)], and the transformation of this DNAzyme into a fluorescent sensor for Na(+) by labeling

164 xt, by integrating the complement of a 10-23 DNAzyme into the primer pair, PCR-amplified DNAzyme-amps

165 The DNAzyme is based on a reported 18-mer G-quadruplex-formi

166 This DNAzyme is highly selective for lanthanides as well, sho

167 The Zn(2+)-dependent ligation DNAzyme is implemented as a biocatalyst for the amplifie

168 he reactivity and global folding of the 8-17 DNAzyme is investigated, and the results are compared wi

169 hybridization with an oligonucleotide-tailed DNAzyme is performed to introduce the DNAzyme to the bio

170 However, the selection of efficient DNAzymes is a challenging process but one that is of cru

171 his work, in vitro selection of RNA-cleaving DNAzymes is carried out using Tl(3+) as the target metal

172 The thriving generation of new DNAzymes is expected to open the door to several healthc

173 The catalytic activity of the DNAzymes is restored in a universal manner in response t

174 ve the catalytic functions of nucleic acids (DNAzymes) is introduced.

175 autonomous synthesis of the Mg(2+)-dependent DNAzyme, is used for the amplified, multiplexed analysis

176 point for interpreting experimental data on DNAzyme kinetics, as well as developing more detailed si

177 dition of hemin to antiparallel G-quadruplex DNAzymes lead to a blue shift in the CD spectra of the G

178 encher-modified substrates by the respective DNAzymes leads to the fluorescence of F1 and/or F2 as re

179 uence (corresponding to the substrate of the DNAzyme) linked to a G-rich domain, which is "caged" in

180 ization to surface-bound capture probes, the DNAzyme-linked LCR products induce electrocatalytic resp

181 anticancer drug, doxorubicin, by the Mg(2+)-DNAzyme-locked pores or by the aptamer-ATP complex-trigg

182 ptasensors using replication/nicking enzymes/DNAzyme machineries is described.

183 y phosphoryl transfer from [gamma-(32)P]GTP, DNAzyme-mediated cleavage yielded two radiolabeled cleav

184 This DNAzyme might be developed for treatment of patients wit

185 It is based on the cleavage of DNAzyme moieties from miRNA-hybridized DNAzyme-capped ca

186 target miRNA is realized through the cleaved DNAzyme moieties-catalyzed oxidation of 3,3',5,5'-tetram

187 These aptamers contain a DNAzyme moiety that is initially maintained in an inacti

188 have accelerated the automated generation of DNAzyme molecules.

189 Control experiments using two 39E DNAzyme mutants revealed a different cleavage pattern of

190 in, we report a silver-specific RNA-cleaving DNAzyme named Ag10c obtained after six rounds of in vitr

191 We previously reported a DNAzyme named Ce13d, which has similar responses to all

192 Among them, a DNAzyme named PSCu10 was studied further.

193 a Ce(4+) salt as the target metal, and a new DNAzyme (named Ce13) with a bulged hairpin structure was

194 One of the representative DNAzymes (named Lu12) was further studied.

195 cludes the horseradish peroxidase (HRP)-like DNAzyme, optimum-length linker (10-mer-length DNA), and

196 rough either metal ion-dependent cleavage by DNAzymes or analyte-dependent structural-switching by ap

197 elected position in the DNA duplex region of DNAzymes or aptamers.

198 suitable functional-DNA partners (aptamers, DNAzymes or aptazymes).

199 e Toffoli and Fredkin gates by the action of DNAzymes or the use of a multifluorophoric platform as a

200 nput target can produce more than one active DNAzyme output.

201 RNA-DNA hybrid strands as substrate for the DNAzyme, PCR-amplified DNAzyme-amps generated in the pre

202 nstrates the use of the peroxidase-mimicking DNAzyme (peroxidase-DNAzyme) as general and inexpensive

203 reaction, hemin-binding peroxidase-mimicking DNAzymes ("peroxidymes") mediate the NADH-driven oxidati

204 sed of glucose oxidase (GOx) and pistol-like DNAzyme (PLDz) to detect glucose levels in tears and sal

205 (ii) The DNAzyme-powered logic gates are made to operate at a fin

206 The system consists of a three-stranded DNAzyme precursor (TSDP), the hybridization of which pre

207 get-recycling and generation of an catalytic DNAzyme product on the electrode probe-functionalized PD

208 eviously been designed that are propelled by DNAzymes, protein enzymes and strand displacement.

209 In addition, induction of DNAzyme provides a new approach in the development of gl

210 ced photocatalytic/peroxidation nanoparticle/DNAzyme reaction cascade that generates a spatiotemporal

211 ork enables the rational design of synthetic DNAzyme regulatory networks, with potential applications

212 The metal ion-driven DNAzyme release of substrates from the pores of MP-SiO(2

213 nts with nine different Mg(2+)-ion-dependent DNAzyme reporter units and the incorporation of a fluore

214 DNAzymes represent a promising new class of nucleic acid

215 gle guanine residues within UV1C extends the DNAzyme's activity into the violet region of the spectru

216 yuridine, resulting in minimal change of the DNAzyme's activity.

217 Precise regulation of the DNAzyme's oxidative catalysis can be achieved by externa

218 he detection of other metal ions using other DNAzymes selected through in vitro selection.

219 The activated DNAzyme sensor is then able to specifically catalyze cle

220 environment, no intracellular application of DNAzyme sensors has yet been reported.

221 of the "always-on" mode of first-generation DNAzyme sensors.

222 A complex consisting of the Pb(2+)-dependent DNAzyme sequence and a ribonuclease-containing nucleic a

223 chemes by integrating a peroxidase-mimicking DNAzyme sequence into the LCR amplification probes desig

224 CPs and detection probes containing multiple DNAzyme sequences (DNAzyme-DPs) brought the target SNP a

225 sequences for two different Mg(2+)-dependent DNAzyme sequences and two different functional hairpin s

226 domains and the respective Mg(2+)-dependent DNAzyme sequences are implemented as nicking/replication

227 p domains of the Mg(2+)- or Zn(2+)-dependent DNAzyme sequences with foreign nucleotides prohibits the

228 synthesis of two different Mg(2+)-dependent DNAzyme sequences.

229 Nt.BbvCI and two different Mg(2+)-dependent DNAzyme sequences.

230 onsisting of the Mg(2+)- or Zn(2+)-dependent DNAzyme sequences.

231 Both DNAzymes showed highly favorable cytotoxic/off-target pr

232 Multi-layered DNAzyme signaling and logic cascades are now reported.

233 emin/G-quadruplex structure as HRP mimicking-DNAzyme significantly improved the catalytic reduction o

234 So far, no RNA-cleaving DNAzymes specific for Cu(2+) are known.

235 n the presence of the analyte the peroxidase-DNAzyme structure is disrupted and does not catalyze the

236 which induced the quencher dye close to the DNAzyme structure.

237 mation of active Mg(2+)- or Zn(2+)-dependent DNAzyme structures through the cooperative formation of

238 ctural fundaments (e.g. first structure of a DNAzyme, structures of ribozyme transition state mimics)

239 cording to this method, the Mg(2+)-dependent DNAzyme subunits displace the ligated product, and the r

240 dergo a structural switch that activates the DNAzyme, such that the binding event can be reported thr

241 icated to various biological applications of DNAzymes, such as sensing, therapeutics, and nanodevices

242 identify specific anti-RV molecules based on DNAzyme technology as candidates to a clinical study.

243 lts in the formation of the Mg(2+)-dependent DNAzyme tethered to a free strand consisting of the targ

244 s and the displacement of a Mg(2+)-dependent DNAzyme that catalyzes the generation of a fluorophore-l

245 on of a trigger RNA into the production of a DNAzyme that degrades an independent RNA substrate, a si

246 A DNAzyme that prevents expression of GATA3 reduces coliti

247 ionally, there are specific sequences called DNAzymes that can fold into tertiary structures that dis

248 cted and quantified by deoxyuridine-modified DNAzymes that underwent UNG-dependent deactivation or ac

249 y and efficacy of SB010, a novel DNA enzyme (DNAzyme) that is able to cleave and inactivate GATA3 mes

250 Another DNAzyme, the 8-17 DNAzyme, which has a similar secondary

251 Taking advantages of smartphone and DNAzyme, the assay provides great potential for its prac

252 his PS-modified oligonucleotide with the Tm7 DNAzyme, the cleavage yield increases to approximately 4

253 fficient catalytic activity of HRP mimicking-DNAzyme, the proposed immunosensor exhibited high sensit

254 articularly the allosteric activation of the DNAzymes through cooperative aptamer-substrate complexes

255 By monitoring the activity change of the DNAzymes through the fluorescence enhancement from the D

256 es allosterically stabilize and activate the DNAzymes, thus allowing the selective release of the flu

257 leaves at the recognition site, allowing the DNAzyme to adopt its active conformation.

258 veal the modus operandi of the original UV1C DNAzyme to be a surprisingly versatile one.

259 y the signal from photocaged Na(+) -specific DNAzyme to detect endogenous Na(+) inside cells is repor

260 The binding strength of the DNAzyme to the aptamer sequence was designed to be less

261 tailed DNAzyme is performed to introduce the DNAzyme to the biosensor.

262 tered for the real-life application of these DNAzymes to provide a foundation for future research.

263 10-23 or Zn(2+) -specific 8-17 RNA-cleaving DNAzymes to regulate the expression of FPs as a new clas

264 on to block site-specific cleavage of RNA by DNAzymes to show that MRM1, MRM2, and RNMTL1 are respons

265 Specifically, we demonstrate the use of DNAzymes to suppress the expression of Clover2, a varian

266 The DNAzyme triggers the polymerization of 3,3'-dimethoxyben

267 The catalytic activity of each DNAzyme unit leads to colorimetric detection and provide

268 Different conjugation modes of the aptamer/DNAzyme units and the availability of different aptamer

269 porter units enhance the formation of active DNAzyme units, thus leading to the isothermal autocataly

270 CA-induced synthesis of the Mg(2+)-dependent DNAzyme units.

271 ic and therapeutic insights brought about by DNAzyme use as nanotools and reagents in a range of basi

272 This method has expanded the DNAzyme versatility for detecting metal ions in biologic

273 GATA3-specific DNAzyme (hgd40) or a control DNAzyme via intrarectal administration, or systemic inje

274 ingle- or double-stranded DNAs, the modified DNAzyme was deactivated when the uracil at the indispens

275 mitation, a photoactivatable (or photocaged) DNAzyme was designed and synthesized, and its applicatio

276 A 28-base DNAzyme was designed to specifically bind to and cleave

277 leotide at the scissile position of the 8-17 DNAzyme was replaced by 2'-O-nitrobenzyl adenosine, rend

278 the indispensable thymidine T2.1 in the 8-17 DNAzyme was replaced with a deoxyuridine, resulting in m

279 Signaling between DNAzymes was achieved using a structured chimeric substr

280 problem, in vitro selection of RNA-cleaving DNAzymes was carried out using a library containing a re

281 subsequently the formation of HRP-mimicking DNAzymes was stimulated by adding hemin molecules.

282 A total of 226 candidate DNAzymes were designed against 2 regions of RV RNA genom

283 Despite many studies since DNAzymes were discovered nearly two decades ago, the met

284 , and a few new Cu(2+)-specific RNA-cleaving DNAzymes were isolated.

285 All DNAzymes were screened for their cleavage efficiency aga

286 itches that consist of nucleic-acid cleaving DNAzymes which are temporarily inactivated by hybridizat

287 We focus here on the well-studied 10-23 DNAzyme, which cleaves mRNA with a catalytic efficiency

288 Another DNAzyme, the 8-17 DNAzyme, which has a similar secondary structure but sho

289 dehybridizes the TSDP to release the active DNAzyme, which then carries out metal-ion-dependent clea

290 n a sample, by exposing a hemin/G-quadruplex DNAzyme, which then catalyzes the generation of chemilum

291 DNAzymes, which are sequences of DNA with catalytic acti

292 a shell consisting of a uranyl-specific 39E DNAzyme whose enzyme strand contains a thiol at the 3' e

293 ting the cleavage site of the Na(+)-specific DNAzyme with a photolabile o-nitrobenzyl group, we achie

294 obe specific uranyl-binding sites in the 39E DNAzyme with catalytically relevant concentrations of ur

295 (2+) and the cleavage of a substrate by 8-17 DNAzyme with Pb(2+) in solution, as well as sensitive DN

296 a few Cu(2+) biosensors were reported using DNAzymes with DNA cleavage or DNA ligation activity.

297 of hemin, form catalytic hemin/G-quadruplex DNAzymes with peroxidase activity.

298 Na(+)-specific, RNA-cleaving deoxyribozyme (DNAzyme) with a fast catalytic rate [observed rate const

299 ognition sequence (aptamer) to the catalytic DNAzyme, yielding a hybrid structure termed "nucleoapzym

300 of hemin into the G-quadruplex structure of DNAzyme yields an active HRP-like activity that catalyze