ライフサイエンスコーパス: E. canis

1 E. canis Fbp had a molecular mass (38 kDa) consistent wi

2 E. canis Fbp was homologous to a family of periplasmic F

3 E. canis gp19 composition consists of five predominant a

4 E. canis gp19 has substantial carboxyl-terminal amino ac

5 E. canis gp36 and E. chaffeensis gp47 were differentiall

6 E. canis gp36 was recognized by early acute-phase antibo

7 E. canis immunodominant 30-kDa major outer membrane prot

8 E. canis, a widely recognized agent of canine ehrlichios

9 E. canis, in addition, had a 6.9-kb locus which containe

10 ns of the 16S rRNA genes from six additional E. canis-positive dog blood specimens and from three poo

11 mers derived from a sequence conserved among E. canis isolates.

12 epitope strongly recognized by serum from an E. canis-infected dog.

13 The route of administration and E. canis inoculum size can influence the course of ehrli

14 ibodies that reacted with E. chaffeensis and E. canis antigens in a pattern different from that of hu

15 Ehrlichia chaffeensis and E. canis have a small subset of tandem repeat (TR)-conta

16 drate was detected on the E. chaffeensis and E. canis recombinant proteins, including the two-repeat

17 membrane protein genes of E. chaffeensis and E. canis, respectively.

18 arily in the periplasm of E. chaffeensis and E. canis.

19 tive proteins in E. chaffeensis (75-kDa) and E. canis (95-kD) whole-cell lysates and supernatants wer

20 roteins reacted with E. chaffeensis P120 and E. canis P140, respectively.

21 e of these gene products in pathogenesis and E. canis transmission as well as in designing a rational

22 E. chaffeensis TRP75 and E. canis TRP95 were immunoprecipitated with anti-pTyr an

23 e VDE was similar to the profiles of VHE and E. canis Oklahoma.

24 Anti-E. canis dog serum reacted strongly with 26- and 24-kDa

25 as concurrence of an increased titer of anti-E. canis immunoglobulin G (IgG) antibody in serum, a dec

26 as a transient and mild increase in the anti-E. canis IgG antibody titer in serum.

27 to identify statistical associations between E. canis infection in dogs and multiple dog-related, hum

28 Both E. canis and an uncharacterized Rickettsia species appea

29 nt study revealed transcription of p30-10 by E. canis in naturally infected ticks and sequence conser

30 VirB9, indicating that VirB9 was produced by E. canis in dogs and was antigenic.

31 virB9 was transcribed by E. canis in dogs, ticks, and cell culture.

32 phase serum antibody responses to whole-cell E. canis lysates and recombinant p28, gp140, and gp200 w

33 of 90, 64, or 47 kDa of the E. chaffeensis, E. canis, and E. muris antigens.

34 e was compared with those of E. chaffeensis, E. canis, and E. muris, only VHE and E. muris induced cl

35 cts with DNA extracted from E. chaffeensis-, E. canis-, or E. phagocytophila-infected samples, confir

36 The assay was used to detect E. canis in canine carrier blood and in experimentally i

37 r p28 genes from two geographically distinct E. canis isolates was completely conserved.

38 ed morulae morphologically resembling either E. canis, E. chaffeensis, or E. muris in monocytes in th

39 ave been attributed to infection with either E. canis or Ehrlichia ewingii.

40 ive and reliable serodiagnostic antigens for E. canis infections.

41 nd sequence conservation of p30-10 genes for E. canis from diverse geographic regions.

42 ne ehrlichiosis cases that were positive for E. canis by immunofluorescent antibody test and in vario

43 ers in serum and blood cultures positive for E. canis occurred as early as 14 days postinoculation.

44 ng the 22 samples that were IFA positive for E. canis, 100% reacted with rP43, 96% reacted with rP28,

45 ges encountered during molecular testing for E. canis detection, highlighting the broader difficultie

46 ensus regarding the positivity threshold for E. canis qPCR assays, we also evaluated if the factors a

47 ipts were detected by Northern blotting from E. canis infected DH82 cells, indicating that the genes

48 an ELISA format using 141 serum samples from E. canis immunofluorescent antibody (IFA)-positive and I

49 (eDsb) proteins were recognized by sera from E. canis-infected dogs but not from E. chaffeensis-infec

50 osylated synthetic peptide repeat units from E. canis gp36 and E. chaffeensis gp47 were substantially

51 gest that dogs serve as a reservoir of human E. canis infection and that R. sanguineus, which occasio

52 ive quantity identified major immunoreactive E. canis antigens recognized early in the infection as t

53 n infection, additional major immunoreactive E. canis proteins were identified, including the 28-, 47

54 present study we cloned a new immunoreactive E. canis surface protein gene of 1,170 bp, which encodes

55 y reported the cloning of two immunoreactive E. canis proteins, P28 and P140, that were applicable fo

56 nsis and the complete TR (24 amino acids) in E. canis.

57 nknown genes, and secA in the omp cluster in E. canis were transcriptionally active in the monocyte c

58 resence of a single copy of the mmpA gene in E. canis and Ehrlichia chaffeensis but not in the human

59 scriptional activity of a five gene locus in E. canis encoding homologous, but non-identical, p28 gen

60 ssion of the remaining paralogs was lower in E. canis cultivated in dog monocyte cell line DH82 at 25

61 lted in the presence of an MmpA band only in E. canis, not in E. chaffeenesis.

62 Eleven out of 14 paralogs in E. canis were transcribed in increasing numbers and tran

63 ocyte cell line DH82 at 25 degrees C than in E. canis cultivated at 37 degrees C.

64 The 28-kDa E. chaffeensis and 30-kDa E. canis native proteins were recognized by 25 IFA-posit

65 d 30 sera reacted with 44- to 110-kDa native E. canis antigens.

66 Western blot analysis of E. canis and E. chaffeensis lysates with the anti-rMmpA

67 0 blood samples from dogs from Ohio (area of E. canis nonendemicity) were examined by nested PCR and

68 s from dogs from Arizona and Texas (areas of E. canis endemicity) and 30 blood samples from dogs from

69 l as the first molecular characterization of E. canis directly from naturally infected ticks.

70 en groups of dogs with log concentrations of E. canis-infected canine-origin cells.

71 scriptionally active in in-vitro cultures of E. canis incubated at the vertebrate host (37 degrees C)

72 ghly sensitive and specific for detection of E. canis and may be more useful in assessing the clearan

73 ix dogs administered the two highest dose of E. canis developed an ehrlichial infection.

74 in the cytoplasm of the reticulate forms of E. canis and Ehrlichia chaffeensis but was notably found

75 Two DNA fragments of E. canis were amplified by PCR with two primer pairs bas

76 epeat units, and the 140-kDa protein gene of E. canis has 14 nearly identical, tandemly arranged 108-

77 t contained a partial 30-kDa protein gene of E. canis.

78 9-kDa major immunoreactive protein (gp19) of E. canis and identified the corresponding TR-containing

79 his is the first molecular identification of E. canis infection in dogs from Peru.

80 sed platelet concentration, and isolation of E. canis by blood culture.

81 iters in serum and the earliest isolation of E. canis from blood.

82 Ehrlichia chaffeensis and p30 gene locus of E. canis despite marked divergence between genera in the

83 stinguishable from disease manifestations of E. canis or E. ewingii.

84 s localized mainly on the morula membrane of E. canis.

85 -kDa major outer membrane proteins (OMPs) of E. canis and E. chaffeensis.

86 chaffeensis, < or =67.3% identity to P30 of E. canis, and < or =63.1% identity to MAP1 of C. ruminan

87 ridization, detecting as little as 0.2 pg of E. canis DNA.

88 ve scenario, we found that the prevalence of E. canis among dogs was 19.6% (95% CI 15.8-23.9%).

89 g the major immunoreactive 36-kDa protein of E. canis and the corresponding ortholog of E. chaffeensi

90 ) of E. chaffeensis, and a 30-kDa protein of E. canis.

91 the 30-kDa major outer membrane proteins of E. canis will greatly facilitate understanding pathogene

92 ominant major outer membrane P30 proteins of E. canis.

93 These data suggest that rMAP2 of E. canis could be used as a recombinant test antigen for

94 found to contain antibodies against rMAP2 of E. canis in the ELISA.

95 ma specimens strongly recognized the rP30 of E. canis.

96 25 samples were positive for a new strain of E. canis.

97 The predicted three-dimensional structure of E. canis Fbp demonstrated conservation of important Fbp

98 that VHE is a new strain or a subspecies of E. canis which may cause asymptomatic persistent infecti

99 ine-rich regions in the acidic C terminus of E. canis TRP95 but not in E. chaffeensis TRP75.

100 e of VHE was most closely related to that of E. canis Oklahoma.

101 ) and was closely related (99.9%) to that of E. canis Oklahoma.

102 the efficacy of doxycycline for treatment of E. canis, E. equi, and E. ewingii infections but indicat

103 t in Iquitos, Peru, interventions to prevent E. canis infection should prioritize dogs living in hous

104 d with rP30 and a 30-kDa protein of purified E. canis.

105 eactions between rP30 and the whole purified E. canis antigen were compared in the dot immunoblot ass

106 We previously demonstrated that recombinant E. canis p28 and the 140- and 200-kDa glycoproteins gp14

107 noblotting demonstrated that the recombinant E. canis p120 reacted with convalescent sera from dogs w

108 humans: Ehrlichia chaffeensis, E. sennetsu, E. canis, and the agent of human granulocytic ehrlichios

109 or Ct <= 40, which has been used in several E. canis studies.

110 Coinfection with three Ehrlichia species (E. canis, E. ewingii, and E. equi) was documented for on

111 to be infected with four Ehrlichia species: E. canis, E. chaffeensis, E. equi, and E. ewingii.

112 dogs infected with microorganisms other than E. canis, were seronegative.

113 The E. canis gp200 gene (4,263 bp; 1,421 amino acids) was cl

114 and p30a) were cloned and sequenced from the E. canis genomic DNA.

115 ientations in a 28- and a 27-kb locus in the E. canis and E. chaffeensis genomes, respectively.

116 nce of multiple copies of these genes in the E. canis genome.

117 s tandem repeats that are not present in the E. canis gp19 gene (414 bp).

118 The overall amino acid sequence of the E. canis p120 is 30% homologous to that of E. chaffeensi

119 1,616 bp), the 14-repeat region (78%) of the E. canis P140 gene (1,620 bp), and a 2-repeat region fro

120 The amino acid homology of the E. canis P28 proteins ranged from 51 to 74%.

121 Like the E. chaffeensis p120, the E. canis p120 contains tandem repeat units.

122 Complete genome sequencing revealed that the E. canis genome consists of a single circular chromosome

123 Phylogenetic analysis among the three E. canis 30-kDa proteins and the major surface proteins

124 The following genes homologous to three E. canis 30-kDa protein genes and the E. chaffeensis omp

125 , a T-helper 1-type response was elicited to E. canis antigens consisting of immunoglobulin G2 antibo

126 y intracellular bacterium closely related to E. canis.

127 -specific primers, sick dogs seroreactive to E. canis antigens were determined to be infected with fo

128 man isolate was ultrastructurally similar to E. canis, E. chaffeensis, and E. muris.

129 Ehrlichia phagocytophila and less similar to E. canis, Ehrlichia ewingii, and E. chaffeensis.

130 s are divided into four clusters and the two E. canis 30-kDa proteins are closely related but that th

131 PCR analysis using E. canis-specific primers revealed that 17 of the 55 dog

132 erapy than IFA, especially in areas in which E. canis is endemic.

133 gs experimentally or naturally infected with E. canis and were previously demonstrated to contain ant

134 es and nymphs) were separately infected with E. canis by feeding on the infected dogs.

135 ned whether dogs and ticks are infected with E. canis in Venezuela and, if so, whether this is the sa

136 er naturally or experimentally infected with E. canis recognized the recombinant protein.

137 from four dogs experimentally infected with E. canis were positive as early as day 4 postinoculation

138 6 weeks in dogs experimentally infected with E. canis.

139 nstrated to contain antibodies reactive with E. canis by indirect immunofluorescence assays.