ライフサイエンスコーパス: E. coli O157

1 E. coli O157 and non-O157 STEC were detected in 35 and 1

2 E. coli O157 can be detected by culture with sorbitol-Ma

3 E. coli O157 causes a wide range of clinical illness tha

4 E. coli O157 isolates clearly segregated into SNP lineag

5 E. coli O157:H7 can cause bloody diarrhea, hemolytic ure

6 E. coli O157:H7 is an enterohemorrhagic bacteria respons

7 E. coli O157:H7 strains possess two lpf loci encoding hi

8 E. coli O157:H7, suspended in 0.1% peptone, was inoculat

9 Low abundant (<100 cells mL(-1)) E. coli O157:H7 cells were isolated and enriched from en

10 using in vitro-generated populations for 10 E. coli O157:H7 strains.

11 a coli O157:H7 from cultures containing 1000 E. coli O157:H7 colony-forming units (cfu)/mL, or approx

12 de polymorphism (SNP) assay to segregate 148 E. coli O157 isolates from Australia, Argentina, and the

13 ia coli strains, CGH microarray data from 19 E. coli O157 pathogenic test strains were used to demons

14 omic analysis of a comprehensive panel of 25 E. coli O157:H7 strains associated with three nearly sim

15 forming units (cfu)/mL, or approximately 500 E. coli O157:H7 and approximately 500 E. coli K12 cfu/mL

16 tects SNPs in 96 loci and applied it to >500 E. coli O157 clinical strains.

17 Stx binding and uptake within the gut after E. coli O157:H7 infection should result in greater disea

18 cts to protect host epithelial cells against E. coli O157:H7 colonization, at least in part, by promo

19 This work demonstrates that not all E. coli O157:H7 strains belong to the same clonal group,

20 1,25(OH)2D3 altered E. coli O157:H7-induced reductions in transepithelial el

21 Although E. coli O157:H7 Sakai contains more than 1,600 genes not

22 Among E. coli O157 isolates, 57 (88%) were identified by both

23 Differences in HUS frequency among E. coli O157:H7 outbreaks have been noted, but our under

24 This study characterized an E. coli O157:H7 strain, designated PA2, that belongs to

25 al gut flora can influence the outcome of an E. coli O157 infection in mice.

26 limit for both glucose (0.08+/-0.02muM) and E. coli O157:H7 ( approximately 4 CFUmL(-1)) were compet

27 athogens (Listeria monocytogenesis 19115 and E. coli O157:H7), clinical isolates (methicillin-resista

28 Escherichia coli, Enterococcus faecalis, and E. coli O157:H7 were compared in fresh (river) water and

29 on and detection of Escherichia coli K12 and E. coli O157 targets.

30 teric human pathogens such as Salmonella and E. coli O157:H7.

31 moeba histolytica, Cryptosporidium spp., and E. coli O157:H7; 95% for Giardia lamblia; 94% for ETEC a

32 e B cell upon cellular membrane anchors anti-E. coli O157:H7 IgM.

33 Antibody (human IgG, anti-E. coli O157:H7, and anti-Salmonella) complexes on the s

34 E. coli O157:H7 have been tested using anti-E. coli O157-magnetic beads conjugate (MBs-pECAb) as a c

35 The films functionalized with anti-E. coli O157:H7 and anti-Salmonella antibodies were used

36 anocomposite platform was modified with anti-E. coli O157:H7 monoclonal antibody.

37 nic E. coli K-12 did not colonize as well as E. coli O157:H7 at the bovine terminal rectal mucosa.

38 one of the 22 identified outbreak-associated E. coli O157:H7 or E. coli O61 pulsed-field gel electrop

39 h 1 of the 22 identified outbreak-associated E. coli O157:H7 or E. coli O61 pulsed-field gel electrop

40 is region showed that all spinach-associated E. coli O157:H7 isolates harbored this same G:C-->A:T su

41 ates, derived from a 2006 spinach-associated E. coli O157:H7 outbreak, were analyzed, all (194/194) d

42 king the inflammatory response to attenuated E. coli O157:H7 adhesion, mucin 2 (MUC2) expression was

43 s (SNPs) were attributable to six "atypical" E. coli O157 strains and included recombinant regions.

44 we performed competition experiments between E. coli O157:H7 and an isogenic DeltalpfA1 DeltalpfA2 do

45 gher resolution of the relationships between E. coli O157 isolates than that provided by MLVA.

46 ed in liquid cultures using a bioluminescent E. coli-O157:H7 strain.

47 e major differences between human and bovine E. coli O157 isolates were due to the relative abundance

48 at support vector machine analysis of bovine E. coli O157 isolate sequences can be applied to predict

49 nly a minor subset (less than 10%) of bovine E. coli O157 isolates analyzed in our datasets were pred

50 epithelial cells is mediated by intimin, but E. coli O157:H7 also possess several other putative adhe

51 was increased by TNF-alpha treatment and by E. coli O157:H7 infection.

52 cultivar-specific effects on colonization by E. coli O157, we used 12 different cultivars of lettuce

53 associated with salad leaves contaminated by E. coli O157.

54 resistance by consuming nutrients needed by E. coli O157:H7 to colonize, thus preventing this first

55 imit the availability of sugars preferred by E. coli O157:H7 and perhaps other pathogens.

56 ntrations of endotoxins and bacterial cells (E. coli O157:H19).

57 jority of cattle excrete less than 10(2) CFU E. coli O157/g feces, most studies, including those usin

58 significantly increased survival of E. coli, E. coli O157:H7 and Ent. faecalis in the water column.

59 Whole genome sequencing was used to compare E. coli O157 isolates from host reservoirs (cattle and s

60 contrast, lacks EIIC(Gam), and consequently, E. coli O157:H7 strains cannot utilize Gam.

61 Here, we constructed E. coli O157:H7 gal mutants which presumably have little

62 The sample containing E. coli O157:H7 was circulated through the system in the

63 In contrast, E. coli O157:H7 strains that were missing Stx or hemolys

64 While the amounts of culturable E. coli O157:H7 rapidly decline after introduction onto

65 oarray format was used to capture and detect E. coli O157:H7.

66 ay be effectively used to capture and detect E. coli O157:H7.

67 ed biosensor was able to specifically detect E. coli O157:H7 at the low concentration within 10 min i

68 dvances are exploited to successfully detect E. coli O157 in a 500-fold higher background of E. coli

69 ti-Salmonella antibodies were used to detect E. coli O157:H7 and S. enteriditis through label-free IR

70 demonstrating the assay's ability to detect E. coli O157:H7 in the presence of high levels of backgr

71 in an enhanced colorimetric signal to detect E. coli O157:H7.

72 ess as low as 20 nm was capable of detecting E. coli O157:H7 and Salmonella sp. in complex hamburger

73 of only 1 Da) was sufficient to distinguish E. coli O157:H7 from a non-O157:H7, nonpathogenic E. col

74 x biomarkers, it was possible to distinguish E. coli O157:H7 from a nonpathogenic E. coli by top-down

75 Antibiotic use during E. coli O157:H7 infections is associated with a higher r

76 etter risk in response to novel and emerging E. coli O157:H7 resistance and virulence phenotypes.

77 ducing Escherichia coli (STEC), encompassing E. coli O157 and non-O157 STEC, is a significant cause o

78 coli strains, including the enterohemorragic E. coli O157:H7.

79 E. coli CFT073 and UTI89, enterohemorrhagic E. coli O157:H7, and enterotoxigenic E. coli O78:H11, co

80 precursor to the virulent enterohemorrhagic E. coli O157:H7.

81 st closely associated with enterohemorrhagic E. coli O157:H7-mediated hemorrhagic colitis that someti

82 orrhagic Escherichia coli (EHEC), especially E. coli O157:H7, is an emerging cause of food-borne illn

83 Fewer E. coli O157:H7 cells survived when applied onto plants

84 One hundred five E. coli O157 strains isolated over a 5-year period from

85 To protect consumers from deadly foodborne E. coli O157:H7 infection, it is vital to develop a simp

86 all pectic substrates were anti-adhesive for E. coli O157:H7 binding to human HT29 cells.

87 ting-flow QCM system, capture antibodies for E. coli O157:H7 were first immobilized onto the QCM chip

88 ble smartphone based fluorescence device for E. coli O157:H7 detection.

89 A major risk factor for E. coli O157:H7 infection is exposure to shallow groundw

90 tery, a competent laboratory should look for E. coli O157:H7 and Shiga toxin directly in stool.

91 ssay involves PCR-amplified gene markers for E. coli O157:H7 (rfbO157, eae, vt1, and vt2) incorporati

92 this new system was as low as 2.3 CFU/mL for E. coli O157:H7 and 5 ng/mL for hepatitis B surface Ag (

93 n, and milk), the LOD was under 5 CFU/mL for E. coli O157:H7, S. typhimurium and L. monocytogenes.

94 fecal pats from 91 farms tested positive for E. coli O157.

95 elationship with a case testing positive for E. coli O157:H7 and coincident diarrheal illness.

96 All samples testing positive for E. coli O157:H7 contained deer feces, and 5 tested farm

97 mental samples from farm A were positive for E. coli O157:H7.

98 which of these options is most relevant for E. coli O157:H7 on leafy green produce, we developed and

99 Cattle are the main reservoir for E. coli O157, and vaccines for cattle now exist.

100 Positive selection for E. coli O157:H7 across the farms identified only one pos

101 ed fresh strawberries as a novel vehicle for E. coli O157:H7 infection, implicated deer feces as the

102 ingesting contaminated food, most frequently E. coli O157:H7 in ground beef or fresh produce.

103 e PCR (qPCR) was used to test for genes from E. coli O157:H7 (eaeO157), shiga-toxin producing E. coli

104 Finally, a homologue of lymphostatin from E. coli O157:H7 (ToxB; L7095) was also found to possess

105 A specific DNA sequence from E. coli O157:H7 having 22 mers as an amine-terminated pr

106 e food contaminant Escherichia coli O157:H7 (E. coli O157:H7) in complex food products due to the rec

107 y could prevent substantial numbers of human E. coli O157 cases.

108 ant for the clinical manifestations of human E. coli O157:H7 infection for their contribution to the

109 f intracellular oxidative stress detected in E. coli O157:H7 illustrated that ROS also played a role

110 nly adaptation and genome diversification in E. coli O157:H7 but also contributing to the development

111 f OtpA into the extracellular environment in E. coli O157:H7 requires OtpB and that when OtpA was pro

112 We inactivated gadE in E. coli O157:H7 Sakai and compared global transcription

113 ce of infection, and therefore, reduction in E. coli O157:H7 prevalence in cattle by vaccination repr

114 ormation is complex and poorly understood in E. coli O157:H7.

115 r the formation of GlcNAc-P-P-Und by WecA in E. coli O157.

116 rotect the gut from colonization by incoming E. coli O157:H7.

117 itol 3-kinase (PI3K)/Akt signaling increased E. coli O157:H7 adherence to HT-29 cells.

118 ikes TSP2, TSP3, and TSP4, CBA120 can infect E. coli O157, O77, and O78, respectively.

119 at cultivar-specific root exudate influences E. coli O157 activity.

120 but was better, and not affected by initial E. coli O157 numbers, above this concentration.

121 their target organisms were used to isolate E. coli O157:H7 and S. typhimurium separately from a coc

122 Enterococcus faecalis, Escherichia coli K12, E. coli O157:H7, Salmonella enterica serovar Typhimurium

123 Finally, utilizing QDs to label E. coli O157:H7 in cell mixtures results in greater accu

124 and rapid method which can detect low level E. coli O157:H7 in foods at real-time.

125 . coli vs. S. enteriditis) and strain level (E. coli O157:H7 vs E. coli K12).

126 of E. coli O157:H7 in buffer and 600 CFU/ml E. coli O157:H7 in liquid food systems.

127 g samples with 10(3), 10(4) and 10(5) CFU/mL E. coli O157:H7 were 106.98, 96.52 and 102.65 (in yogurt

128 complement complex, was protective in murine E. coli O157:H7 infection.

129 ferent from other Stx-positive and -negative E. coli O157:H7 strains and were more similar to MG1655

130 30%), culturable amounts of the nontoxigenic E. coli O157:H7 strain ATCC 700728 and the virulent stra

131 ied by enzymes in the E. coli serotype O157 (E. coli O157) O-PS biosynthetic pathway.

132 Disinfection experiments showed that 73% of E. coli O157:H7 died within 2 h with a disinfection rate

133 sensor was developed based on the ability of E. coli O157:H7 proteases to change the optical response

134 The colonizing ability of E. coli O157:H7 was compared with those of nonpathogenic

135 phere, and the higher number and activity of E. coli O157 cells in the rhizosphere may be a consequen

136 Adherence of E. coli O157 : H-expressing flagella of serotype H7, H6

137 rotein and resulted in decreased adhesion of E. coli O157:H7.

138 indicate that GadE is critical for the AR of E. coli O157:H7 and that it plays an important role in v

139 The binding of E. coli O157:H7 to the IgM on B cell surface activates t

140 s pre-dates the first reported human case of E. coli O157:H7, which was in 1975 from the United State

141 for enteric culture (0.7%), with 65 cases of E. coli O157.

142 The cells of E. coli O157:H7 specifically captured and enriched on th

143 e used to identify sublineages and clades of E. coli O157, and when they were correlated with the cli

144 ted in intestinal-epithelial colonization of E. coli O157:H7 and may represent a useful target for va

145 sal E. coli strains prevents colonization of E. coli O157:H7 in a mouse model.

146 Extremely low concentration of E. coli O157:H7 (~10 CFU/mL) could be detected within 1h

147 ) for indicating different concentrations of E. coli O157:H7 and smart phone imaging APP for monitori

148 Concentrations of E. coli O157:H7 from 3x10(1) to 3x10(7)cfu/mL could be d

149 inoculated with different concentrations of E. coli O157:H7 have been tested using anti-E. coli O157

150 g mixture distribution analysis on counts of E. coli O157.

151 rect plating on SMACct, the direct counts of E. coli O157:H7 were highly correlated with the estimate

152 The sensitivity of detection of E. coli O157 by both direct plating and IMS was highly d

153 Sensitivity of detection of E. coli O157 in bovine feces at low initial concentratio

154 n adequate stand-alone test for detection of E. coli O157 in clinical samples.

155 Finally, the sensitive detection of E. coli O157 in the presence of K12 at a ratio of 1:1000

156 1 and stx2) (including specific detection of E. coli O157), Shigella spp./enteroinvasive E. coli, Cry

157 is study, we reported the rapid detection of E. coli O157:H7 by using calcium signaling of the B cell

158 mical immunosensor for specific detection of E. coli O157:H7 contamination with the use of sandwich a

159 que and sensitive tools for the detection of E. coli O157:H7 from food as they are host-specific and

160 r phage assay shows promise for detection of E. coli O157:H7 from food in a simple, fast and sensitiv

161 specificity and sensitivity for detection of E. coli O157:H7 in chicken samples with a lower detectio

162 The detection of E. coli O157:H7 in foods has held the attention of many

163 ased biosensor was used for the detection of E. coli O157:H7 in ground beef samples.

164 uffer pH, and assay time on the detection of E. coli O157:H7 were investigated and optimized.

165 E. coli O157:H7, the sensitive detection of E. coli O157:H7 were realized both in standard samples a

166 d bands and thus, enable visual detection of E. coli O157:H7 without instrumentation.

167 s highly desirable for specific detection of E. coli O157:H7, one of the leading bacterial pathogens

168 PAD) has been developed for the detection of E. coli O157:H7, Salmonella Typhimurium, and L. monocyto

169 ped for sensitive and selective detection of E. coli O157:H7.

170 for the visual and quantitative detection of E. coli O157:H7.

171 oxide and quantum dots for determination of E. coli O157:H7 in beef and river water.

172 ensive studies of the global distribution of E. coli O157 lineages and the impacts of regionally pred

173 in the current international distribution of E. coli O157:H7, and it is likely that these events were

174 es evidence for the geographic divergence of E. coli O157 and for a prominent role of stx2a in total

175 idization and used to study the diversity of E. coli O157 isolates from human infections and food and

176 In particular, the capture of genomic DNA of E. coli O157:H7 by a specific oligonucleotide probe coat

177 eep were treated with biweekly oral doses of E. coli O157:H7 (an STEC) or an isogenic stx mutant stra

178 proved our understanding of the evolution of E. coli O157:H7 and suggested a key revision to accommod

179 f the plant can also influence the extent of E. coli O157 colonization.

180 logues of LpxR are present in the genomes of E. coli O157:H7, Yersinia enterocolitica, Helicobacter p

181 Using comparative genomics of E. coli O157:H7 subpopulations, we have identified one s

182 n and a complete inhibition of the growth of E. coli O157:H7 in the later 24 h irradiation.

183 in a specific test for the identification of E. coli O157:H7, distinguishing it from other pathogenic

184 on and subsequent disease after ingestion of E. coli O157:H7 may depend, at least in part, on individ

185 g human consumption, leaf internalization of E. coli O157 may pose more of a public health risk than

186 ypic analyses of 120 independent isolates of E. coli O157:H7 from our culture collection revealed tha

187 and rapid technique to detect low levels of E. coli O157:H7 using membrane filtration and silver int

188 entify SLT-1 from the complex cell lysate of E. coli O157:H7.

189 ter inoculation onto plants, the majority of E. coli O157:H7 cells either die or are no longer cultur

190 thereby facilitating genetic manipulation of E. coli O157:H7.

191 lie the molecular pathogenetic mechanisms of E. coli O157:H7.

192 GA also cause injury to the cell membrane of E. coli O157:H7.

193 he pLFS could detect as low as 100 CFU/ml of E. coli O157:H7 in buffer and 600 CFU/ml E. coli O157:H7

194 dality, was investigated in a mouse model of E. coli O157:H7 infection.

195 del of the regulation of the lpfA1 operon of E. coli O157:H7 by H-NS and Ler is discussed.

196 In 2006, in an outbreak of E. coli O157:H7 caused by consumption of contaminated sp

197 09, we investigated a multistate outbreak of E. coli O157:H7 infections.

198 The pathogenicity of E. coli O157:H7 is mainly caused by the expression of Sh

199 The pathogenicity of E. coli O157:H7 was also investigated in rabbits.

200 IgG against the O-specific polysaccharide of E. coli O157:H7 may confer immunity by lysing the inocul

201 hat bulk anise oil reduced the population of E. coli O157:H7 and L. monocytogenes by 1.48 and 0.47 lo

202 t Scotland were examined for the presence of E. coli O157.

203 Irrespective of the presence of E. coli O157:H7, TNF-alpha enhanced activation of p65, t

204 ably grossly underestimate the prevalence of E. coli O157 in cattle.

205 control measures to reduce the prevalence of E. coli O157.

206 servation even in low concentration range of E. coli O157:H7.

207 -PCR results prompted successful recovery of E. coli O157 (n = 25) and non-O157 STEC (n = 8) isolates

208 tract of cattle is the primary reservoir of E. coli O157:H7, and thus, it is critical to eliminate o

209 (2) The retention of E. coli O157:H7 was 3.3 fold higher than that of E. coli

210 Considering the health risks of E. coli O157:H7 presence in food and water, an affordabl

211 detection limit, 100 CFU g(-1) dry soil) of E. coli O157:H7 was observed in the soils from Salinas V

212 . coli strains but absent from MS spectra of E. coli O157:H7 strains was identified by top-down analy

213 t is crucial to reduce the further spread of E. coli O157:H7 and other (emerging) STEC strains global

214 magnifying the imbalance of redox status of E. coli O157:H7.

215 tion of a nalidixic acid-resistant strain of E. coli O157 in bovine feces was assessed by culture on

216 ed with a chromosomally lux-marked strain of E. coli O157:H7.

217 cspC, ybgS, yahO, and yjbJ for 11 strains of E. coli O157:H7 and 7 strains of the "near-neighbor" ser

218 detection strategy for pathogenic strains of E. coli O157:H7 serotype based on a conserved signature

219 studies, we have determined that a subset of E. coli O157 infections will not be detected if an agar-

220 etection and epidemiological surveillance of E. coli O157, and the data were used to identify discern

221 suitable for epidemiological surveillance of E. coli O157.

222 a functional GAD system and for survival of E. coli O157:H7 in a simulated gastric environment.

223 nd EC significantly affected the survival of E. coli O157:H7 in leafy green producing soils and the d

224 n indirect effect in the overall survival of E. coli O157:H7 in soils.

225 n sediments, survival of FIB but not that of E. coli O157:H7 increased in disinfected treatments, ind

226 Here, the transmission of E. coli O157 within a typical UK dairy herd is modelled

227 wt % led to a reduction in the transport of E. coli O157:H7 and K12 from 98 to 10% and from 95 to 70

228 ar into QS columns enhanced the transport of E. coli O157:H7 by 3.1 fold compared to the unoxidized c

229 e 1 trial in adults showed that a vaccine of E. coli O157:H7 O-specific polysaccharide conjugated to

230 tribution of GadE to the AR and virulence of E. coli O157:H7 remains largely unknown.

231 ic microbiota could enhance the virulence of E. coli O157:H7, particularly a subset of clade 8 strain

232 inhibitory effect of C70-TiO2 thin films on E. coli O157:H7 showed a decrease of the bacterial conce

233 ns previously tested for Stx (by EIA) and/or E. coli O157:H7 (by culture) were tested by PCR.

234 Otherwise, E. coli O157:H7 only growth 50% when PBE was added to th

235 ique enables digital detection of pathogenic E. coli O157 cells in a high background of normal K12 ce

236 cterial cell mixtures composed of pathogenic E. coli O157:H7 and harmless E. coli DH5alpha using flow

237 immunosensor for the detection of pathogenic E. coli O157:H7.

238 he electrical properties once the pathogenic E. coli O157:H7 captured on the sensor surface.

239 as the fluorophore label for the pathogenic E. coli O157:H7 serotype: limits of 1% O157:H7 in 99% DH

240 competitive advantage in vivo to pathogenic E. coli O157:H7 and commensal E. coli K-12, whereas degr

241 ultaneous testing of the foodborne pathogens E. coli O157:H7 and Salmonella enterica, in detail a nuc

242 e host inflammatory response acts to perturb E. coli O157:H7 intestinal colonization.

243 Once infected by the PhiV10 reporter phage, E. coli O157:H7 produces a strong bioluminescent signal

244 fy viable (with PMA) and total (without PMA) E. coli O157:H7 cells on growth chamber and field-grown

245 es and the impacts of regionally predominant E. coli O157 lineages on the prevalence and severity of

246 ), and EIID(Aga) of the Aga PTS are present, E. coli O157:H7 strains normally are able to utilize Aga

247 o virulence factors of Shiga toxin-producing E. coli O157:H7.

248 thus, it is critical to eliminate or reduce E. coli O157:H7 gut colonization.

249 ul component in a systemic vaccine to reduce E. coli O157:H7 colonization in cattle.

250 dition anise oil nanoemulsion (AO75) reduced E. coli O157:H7 and L. monocytogenes count by 2.51 and 1

251 o epithelial cells and significantly reduced E. coli O157 excretion levels from sheep.

252 onize new niches, interrogation of sequenced E. coli O157:H7 genomes showed a high level of CycA cons

253 ere found to be related to the two sequenced E. coli O157:H7 strains, EDL933 and Sakai.

254 eas closely related sorbitol-fermenting (SF) E. coli O157:H(-) strains carry plasmid pSFO157 (>120,00

255 Similarly, E. coli O157:H7 adhesion to cattle colonic explants was

256 e method is gram negative pathogenic species E. coli O157:H7.

257 chieved in less than 30 min at both species (E. coli O157:H7 vs S. typhimurium ) and strain (E. coli

258 l of Enterococcus faecalis, Salmonella spp., E. coli O157 and porcine parvovirus in bioreduction vess

259 ETEC], Shiga toxin-producing E. coli [STEC], E. coli O157:H7, Vibrio cholerae, Yersinia enterocolitic

260 coli O157:H7 vs S. typhimurium ) and strain (E. coli O157:H7 vs E. coli K12) levels in complex food m

261 extract and diallyl compounds treatment than E. coli O157:H7.

262 d non-O157 STEC in clinical samples and that E. coli O157:H7 remains the predominant cause of HUS in

263 Given that E. coli O157:H7 produces effectors that attenuate inflam

264 and stepwise regression analysis showed that E. coli O157:H7 survival in soils was negatively correla

265 The E. coli O157:H7 bacteriophage PhiV10 was modified to exp

266 The E. coli O157:H7 best able to persist had intimin, Tir, a

267 The E. coli O157:H7 strain EDL933 carries two copies of non-

268 ulator of the LEE, encoded by ecs1581 in the E. coli O157:H7 strain Sakai genome and present but not

269 We demonstrate that recognition of the E. coli O157:H7 host by CBA120 involves binding to and d

270 tudy and are now available for typing of the E. coli O157:H7 lineage.

271 -NS) binds to the regulatory sequence of the E. coli O157:H7 lpf1 operon and "silences" its transcrip

272 better understanding of the evolution of the E. coli O157:H7 pathogenome, the present study presents

273 osidase assay results, we concluded that the E. coli O157:H7 lpf operon possesses a promoter dependen

274 imit of 0.2 cfu/microL is achieved using the E. coli O157 target and an input volume of 50 microL.

275 nsification of color was correlated with the E. coli O157:H7 concentration.

276 and the specific recognition of antibody to E. coli O157:H7, the sensitive detection of E. coli O157

277 was isolated, 28 (90%) were attributable to E. coli O157 and 3 (10%) were attributable to non-O157 S

278 a differential cultivar-specific response to E. coli O157 colonization, although importantly there wa

279 h graphene based capacitors were specific to E. coli O157:H7 strain with a sensitivity as low as 10-1

280 e TSP that confers phage specificity towards E. coli O157:H7.

281 Unfortunately, E. coli O157 cannot be genetically manipulated using the

282 Enrichment assays using E. coli O157:H7 grown in LB broth with a reporter phage

283 hough the feasibility was demonstrated using E. coli O157:H7 as a model analyte, this approach could

284 The real-time monitoring method for viable E. coli O157:H7 developed in this study can be used to e

285 of NF-kappaB inflammatory signaling, whereas E. coli O157:H7 infection suppressed this pathway by inh

286 defined confirmed cases as persons from whom E. coli O157:H7 with the outbreak PFGE pattern was cultu

287 responsible for most deaths associated with E. coli O157 infection.

288 r treatment of complications associated with E. coli O157:H7 infection.

289 capacitors that were biofunctionalized with E. coli O157:H7 specific antibodies for sensitive pathog

290 Following subsequent oral challenge with E. coli O157:H7, reduced colonization rates and delayed

291 We measured Ang-1/2 in 77 children with E. coli O157:H7 infection.

292 C for 30 min and subsequently incubated with E. coli O157:H7 for 30 min to achieve a 3.2 +/- 0.2 log

293 Fifteen percent of patients infected with E. coli O157:H7 progress to hemolytic uremic syndrome, b

294 %) or low (2%) fiber diets and infected with E. coli O157:H7 strain 86-24 (Stx2+).

295 We enrolled children infected with E. coli O157:H7 within 1 week of the onset of diarrhea i

296 Finally, in children infected with E. coli O157:H7, plasma SDF-1 levels were elevated in in

297 occurs after gastrointestinal infection with E. coli O157:H7, which produces Shiga toxins (Stx) that

298 human and experimental bovine infection with E. coli O157:H7.

299 cient C57BL/6 mice were also inoculated with E. coli O157:H7 and only 1 of 14 developed disease, wher

300 In experiments using meats spiked with E. coli O157:H7, colicins efficiently reduced the popula