ライフサイエンスコーパス: EF-1alpha

1 EF-1alpha complexes with a dissociation constant K(d) =

2 EF-1alpha expression, but not EF-1beta or -gamma express

3 EF-1alpha was found to form a stable complex with leucyl

4 escribed proteins, elongation factor 1alpha (EF-1alpha) and the polypyrimidine tract-binding protein

5 ryotic translation elongation factor 1alpha (EF-1alpha) as the primary target for this compound class

6 nd the translation elongation factor 1alpha (EF-1alpha) gene.

7 polypeptide chain elongation factor 1alpha (EF-1alpha) promoters in the pcDNA 3-based expression vec

8 genes: translation elongation factor 1alpha (EF-1alpha), the largest subunit of RNA polymerase (RPB1)

9 in-binding protein elongation factor 1alpha (EF-1alpha).

10 b (cyt b), nuclear elongation factor-1alpha (EF-1alpha) and two anonymous nuclear loci-for 14-15 Fren

11 Polypeptide elongation factor-1alpha (EF-1alpha) is a multifunctional protein whose levels are

12 sette to the human elongation factor-1alpha (EF-1alpha) promoter has permitted successful establishme

13 ith the eukaryotic elongation factor-1alpha (EF-1alpha).

14 S, suggesting the existence of a larger aaRS:EF-1alpha complex in archaea.

15 In addition, EF-1alpha co-purified with the archaeal multi-synthetase

16 f the translation elongation factor 1 alpha (EF-1alpha) and actin (Act) genes.

17 o the ribosome by elongation factor 1 alpha (EF-1alpha).

18 plant (wheat germ) proteins: aminoacylation, EF-1alpha*GTP binding, and 3'-adenylation of 3'-CC forms

19 t and fluorescent reporter proteins under an EF-1alpha promoter.

20 With Northern blot analysis, EF-1alpha expression was enhanced by 2- to 6-fold in bot

21 the French Pyrenees, but for both cyt b and EF-1alpha there was then a step change to the upland Mas

22 tiated by coimmunoprecipitation of CCaMK and EF-1alpha using the protein extract from lily anthers.

23 in EF-Tu with sequence homologues, EF-G and EF-1alpha, suggests steric clashes that would prevent th

24 n, chaperonin 60, valyl-tRNA synthetase, and EF-1alpha, suggests a sister-group relationship between

25 To investigate possible interactions between EF-1alpha and other cellular components, a yeast two-hyb

26 These interactions between EF-1alpha and the archaeal MSC contribute to translation

27 Interactions between EF-1alpha and various aaRSs have been described in eukar

28 ther fungi, and is unique in possessing both EF-1alpha and EFL genes.

29 appears to involve an interaction with both EF-1alpha and ribosomes in which all three components ar

30 of this process by regenerating a GTP-bound EF-1alpha necessary for each elongation cycle.

31 , since removal resulted in tight binding by EF-1alpha.GTP.

32 ent protein during latency from the cellular EF-1alpha promoter and the red fluorescent protein (RFP)

33 product, the translational elongation factor EF-1alpha F1, could play a role in the translational reg

34 resented in support of the Grasmuk model for EF-1alpha function in which this elongation factor does

35 up to 3.0-fold increased levels of mRNA for EF-1alpha, -beta, and -delta when compared with normal c

36 The pattern of tRNA interaction observed for EF-1alpha (eEF1A) therefore closely resembles that of ba

37 ocation displays an absolute requirement for EF-1alpha; however, the dependence upon EF-1alpha was pr

38 ryotic elongation factor 1A (eEF1A, formerly EF-1alpha), the protein that forms a complex with GTP an

39 Dissociation of CCaMK from EF-1alpha by Ca2+ and phosphorylation of EF-1alpha by CC

40 y the promoter of either a housekeeping gene EF-1alpha or the human HLA-DRalpha gene, which is select

41 translation elongation factor 1 alpha gene (EF-1alpha), and the second largest subunit of RNA polyme

42 or the binding of various RNAs by wheat germ EF-1alpha.GTP.

43 from one clone were 100% identical to human EF-1alpha.

44 thionine, induced a 1.5-2.0-fold increase in EF-1alpha, -beta, and -delta mRNA.

45 Two pH-sensitive actin-binding sequences in EF-1alpha are identified and are predicted to overlap wi

46 Both EGF and heregulin-beta1 (HRG) induced EF-1alpha promoter activity and mRNA and protein express

47 six genomic regions (LSU rRNA, mtSSU, MCM7, EF-1alpha, Act and ITS) were used for reconstructing the

48 Growth factor-mediated EF-1alpha expression was effectively blocked by pretreat

49 ombinant subunits was determined with native EF-1alpha by measuring stimulation of the rate of elonga

50 The ability of EF-1alpha to bind either F-actin or aa-tRNA in competiti

51 died the effect of F-actin on the ability of EF-1alpha to bind to aa-tRNA.

52 -actin is sufficient to shift the binding of EF-1alpha from F-actin to aa-tRNA as pH increases.

53 wever, the sharp pH dependence of binding of EF-1alpha to F-actin is sufficient to shift the binding

54 We demonstrate that binding of EF-1alpha:GTP to aa-tRNA is not pH sensitive with a cons

55 vo will supply a high local concentration of EF-1alpha to facilitate polypeptide elongation by the F-

56 le in regulating the biological functions of EF-1alpha.

57 uences for translation of the interaction of EF-1alpha with F-actin, we have studied the effect of F-

58 An increased level of EF-1alpha mRNA was also observed in nonobese diabetic mi

59 rom EF-1alpha by Ca2+ and phosphorylation of EF-1alpha by CCaMK in a Ca2+/calmodulin-dependent manner

60 This suggests that the phosphorylation of EF-1alpha by these two kinases may have different functi

61 These results suggest that regulation of EF-1alpha expression by extracellular signals that funct

62 cancers and that growth factor regulation of EF-1alpha expression involve histone acetylation.

63 that pH-regulated recruitment and release of EF-1alpha from actin filaments in vivo will supply a hig

64 constitutive and HRG-induced stimulation of EF-1alpha promoter activity in MCF-7 cells.

65 for growth factor-mediated transcription of EF-1alpha.

66 with the anti-cancer compound didemnin B on EF-1alpha.

67 Complex formation had little effect on EF-1alpha activity, but increased the k(cat) for Leu-tRN

68 1betagammadelta catalyzes exchange of GDP on EF-1alpha with GTP.

69 talyzes the exchange of bound GDP for GTP on EF-1alpha, a required step to ensure continued protein s

70 putatively functionally overlapping paralog EF-1alpha.

71 Recombinant EF-1alpha, PTB, and SRB produced from vaccinia expressio

72 critical function of EF-1beta in regulating EF-1alpha activity, cell growth, translation rates, and

73 s EF-2 binding to pre-translocative ribosome.EF-1alpha complexes, thus preventing ribosomal transloca

74 lection to bacterial EF-Tu formed an Ala-RNA.EF-1alpha.GTP complex with a Kd of 29 nM, indicating tha

75 partial DNA sequences from three loci (RPB2, EF-1alpha, and nuclear ribosomal rRNA) totaling 3.48 kb

76 e reference genes (RPS18, RPS5, RPL32, RPL8, EF-1alpha, beta-Actin, and GAPDH) in the small carpenter

77 The betagammadelta complex stimulated EF-1alpha activity up to 10-fold with a ratio of 20alpha

78 is also consistent with the observation that EF-1alpha's binding to F-actin and aa-tRNA is mutually e

79 pression was under the control of either the EF-1alpha or the metallothionein 1 promoter.

80 dence to suggest that HRG stimulation of the EF-1alpha promoter involves increased physical interacti

81 the observed HRG-mediated regulation of the EF-1alpha promoter.

82 aminoacylation can be directly handed off to EF-1alpha for the next step of protein synthesis.

83 ove tymoviral RNAs in binding more weakly to EF-1alpha*GTP.

84 t is shown here that DB binds only weakly to EF-1alpha/GTP in solution, but binds to ribosome.

85 for EF-1alpha; however, the dependence upon EF-1alpha was previously unexplained.

86 and having space to interact precisely with EF-1alpha, suggesting that the product of aminoacylation