ライフサイエンスコーパス: EMC

1 EMC and Notch were both required in the main body cells

2 EMC expression in the main body cells is Notch dependent

3 EMC expression is reduced in the precursors of the polar

4 EMC has an established role in the insertion of transmem

5 EMC is expressed in proliferating cells in the germarium

6 EMC mutants have a significant reduction in the amount o

7 EMC-6 is ubiquitously expressed and localizes to the ER.

8 we observed that blocking Ship1/2 abrogated EMC cell survival by exerting dual effects on the BCR si

9 demonstrated that inactivation of additional EMC members in C. elegans also impaired AChR synthesis a

10 ls derived from RXRalpha(-/-) embryos and an EMC subline constitutively expressing a dominant negativ

11 n ER-shaping protein localized to EMCs as an EMC promoter.

12 enabled the evaluation of the dynamics of an EMC and precise examination of its position with respect

13 These structures provide a view of an EMC-client complex and define EMC sites-the transmembran

14 ng multipass protein synthesis: the GET- and EMC-like (GEL), protein associated with translocon (PAT)

15 res reveal a Ca(V) assembly intermediate and EMC client-binding sites that could have wide-ranging im

16 pase-3 cleavable PDCs, RGDEVD-DOX (TPD1) and EMC-KGDEVD-DOX (MPD1), for targeting metastatic triple-n

17 ated their strong cross-reactivity with anti-EMC-RBD antibodies.

18 t heterochromatin domain (HOOD) formation at EMC-target loci.

19 ly, we showed a physical interaction between EMC and viral NS4B and that the NS4A-4B region adopts an

20 of the antiapoptotic protein Mcl-1, in both EMC cell lines and primary IgH.TEmu CLL cells.

21 s about the mechanism(s) of TMD insertion by EMC, with various biochemical and proteomics studies beg

22 n opportunity to insert those SAs skipped by EMC.

23 ly short lumenal domains are translocated by EMC, and constrain models of EMC's proposed chaperone fu

24 The underlying causes of endometrial cancer (EMC) are poorly understood, and treatment options for pa

25 Etiology of endometrial cancer (EMC) is not fully understood.

26 recurrent or persistent endometrial cancer (EMC).

27 en EC is paired with ethyl methyl carbonate (EMC), poly(ethylene glycol) dimethyl ether, poly(ethylen

28 Delays in emergency medical care (EMC) can increase sepsis mortality.

29 In this work, Ensemble Monte Carlo (EMC) simulations are used to model the behavior of a gat

30 Expanded monocyte cells (EMC), the immature (ER-MP12(+)) and more mature (ER-MP20

31 s been previously reported that the cellular EMC supports the biogenesis of NS4A and NS4B from Dengue

32 We found that the Saccharomyces cerevisiae EMC contains eight subunits (Emc1-6, Emc7 and Emc10), ha

33 creating an extracellular membrane channel (EMC) to the outside of the cell.

34 creating an extracellular membrane channel (EMC) to the outside of the cell.', and should have read

35 creating an extracellular membrane channel (EMC) to the outside of the cell.'.

36 charged (ESC) and enhanced multiply charged (EMC) scans to perform a gas-phase separation of singly c

37 ion of extraskeletal myxoid chondrosarcomas (EMC) have been shown to have a characteristic translocat

38 nd includes enzymes of the ethylmalonyl-CoA (EMC) pathway that were recently shown to operate during

39 iometry of the multi-subunit protein complex EMC.

40 endoplasmic reticulum (ER) membrane complex (EMC) cooperates with the Sec61 translocon to co-translat

41 e show that loss of the ER membrane complex (EMC) or mutation of the Sec61 translocon causes reduced

42 tein endoplasmic reticulum membrane complex (EMC), is required for surfactant homeostasis in alveolar

43 the recently identified ER membrane complex (EMC).

44 protein Mmi1 to form the Erh1-Mmi1 complex (EMC) implicated in gametogenic gene silencing.

45 etric complex, called the Erh1-Mmi1 complex (EMC), to promote meiotic mRNA decay and facultative hete

46 a member of the ER membrane protein complex (EMC) called EMC1 promotes SV40 ER membrane transport and

47 on the cellular ER membrane protein complex (EMC) for biogenesis.

48 f the conserved ER membrane protein complex (EMC) has decreased phosphatidylserine (PS) transfer from

49 th YidC and the ER membrane protein complex (EMC) implicates an evolutionarily conserved insertion me

50 ctor termed the ER membrane protein complex (EMC) in several aspects of membrane protein biogenesis.

51 channel or the ER membrane protein complex (EMC) insertase.

52 mic reticulum (ER) membrane protein complex (EMC) is a conserved co- and posttranslational insertase

53 mic reticulum (ER)-membrane protein complex (EMC) is a multi-protein transmembrane complex composed o

54 mic reticulum (ER) membrane protein complex (EMC) is an ER multiprotein complex that affects a wide r

55 The ER membrane protein complex (EMC) is an essential insertase that requires seven membr

56 e show that the ER membrane protein complex (EMC) is indispensable for the expression of viral polypr

57 4 and EMC7, two ER membrane protein complex (EMC) subunits, support SV40 infection by promoting LE-to

58 mic reticulum (ER) membrane protein complex (EMC) was identified over a decade ago in a genetic scree

59 e the conserved ER membrane protein complex (EMC) was shown to be essential for efficient insertion i

60 oplasmic reticulum membrane protein complex (EMC)(8,9)-and of the assembled Ca(V)1.2-Ca(V)beta(3)-Ca(

61 mic reticulum (ER)-membrane protein complex (EMC), an evolutionarily conserved complex that has been

62 insertase, the ER membrane protein complex (EMC), and developed a dual-guide approach to identify ge

63 The ER membrane protein complex (EMC), comprising eight conserved subunits, has emerged a

64 Dubbed the ER membrane protein complex (EMC), its disruption has since been found to affect wide

65 oplasmic reticulum membrane protein complex (EMC), which has roles in ER-associated protein biogenesi

66 insertase, the ER membrane protein complex (EMC).

67 , including the ER membrane protein complex (EMC).

68 mic reticulum (ER) membrane protein complex (EMC).

69 , we created an engineered muscle construct (EMC) composed of GLUT4-overexpressing (OEG4) cells.

70 asmic reticulum (ER)-mitochondrial contacts (EMC) regulate diverse cellular activities such as metabo

71 -2)) and lower equilibrium moisture content (EMC), thus showing potential for active food packaging m

72 new method called expected Markov counting (EMC) that produces maximum-likelihood estimates of subst

73 and Riyadh were not different from MERS-CoV EMC/2012 in replication, escape of interferon response,

74 ients with essential mixed cryoglobulinemia (EMC; all with type II), 50 hospital control patients, an

75 In response to LPS, LTA, and Pam(3)Cys (EMC Microcollection GmbH, Tubingen, Germany), CCL20 leve

76 e a view of an EMC-client complex and define EMC sites-the transmembrane (TM) and cytoplasmic (Cyto)

77 Our study delineates EMC requirements in gene silencing and identifies an ERH

78 ls with rapidly and spontaneously developing EMC will help explore mechanisms of cancer initiation an

79 In contrast, the linear carbonates (DMC, EMC, and DEC) exhibited minimal reactivity under the sam

80 four widely used alkyl carbonates (EC, DMC, EMC, and DEC) with singlet oxygen, peroxide, and superox

81 ethyl carbonate: ethyl methyl carbonate (EC: EMC = 3:7) as the electrolyte.

82 Like the RBD of prototype EMC2012 (EMC-RBD), all five RBDs maintained good antigenicity and

83 ctron microscopy structure of the eukaryotic EMC.

84 cross-regulated and cooperatively exacerbate EMC.

85 the ED" was the only significant barrier for EMC use.

86 licity of the client pocket are required for EMC function.

87 coding membrane proteins, a central role for EMC as a TMD insertion factor can explain its high abund

88 Recent studies suggest a role for EMC during membrane protein biogenesis.

89 slocation appears to be largely specific for EMC, but has not been detected in all such tumours.

90 s a potential path for substrate egress from EMC.

91 To perform these functions, EMC associates with two distinct complexes, Mtl1-Red1 co

92 hCoV-EMC/2012 was sensitive to both interferon-alpha2b and ri

93 The virus (called HCoV-EMC) replicated readily in cell culture, producing cytop

94 n-alpha2b and ribavirin on nCoV isolate hCoV-EMC/2012 replication in Vero and LLC-MK2 cells was evalu

95 infectious dose of the MERS-CoV isolate HCoV-EMC/2012, rhesus macaques developed a transient lower re

96 n coronavirus-Erasmus Medical Center or hCoV-EMC), emerged in the Arabian Peninsula.

97 How EMC accomplishes this feat has been unclear.

98 How EMC and Sec61 collaborate to ensure SA insertion in the

99 Yet, we have limited understanding of how EMC enables insertion and integrity of diverse clients,

100 These findings suggest how EMC facilitates energy-independent membrane insertion of

101 Here, yeast and human EMC cryo-EM structures reveal conserved intricate assemb

102 pamycin, in mouse models of EMC and in human EMC cell lines to explore the interactive roles of Cox2

103 ne the composition and architecture of human EMC using biochemical assays, crystallography of individ

104 because they mimic several features of human EMC.

105 We determined the structure of the human EMC in a lipid nanodisc to an overall resolution of 3.4

106 ng an improved structural model of the human EMC, we used mutagenesis and site-specific crosslinking

107 unctions as an assembly factor for the human EMC.

108 f this study (Emek Medical Center Ethics ID: EMC-19-0179).

109 In sepsis-related vignettes, the immediate EMC option ("Call emergency medical services or go to th

110 in homologue gene are frequently detected in EMC.

111 DENV and ZIKV that were serially passaged in EMC knockout cells and find that none are completely ind

112 in, and their activities are up-regulated in EMC.

113 onal deletion of endometrial Pten results in EMC in all female mice as early as age 1 month with myom

114 Indeed, EMC targets masked in mmi1Delta due to termination defec

115 ent-specific regulation, and a non-insertase EMC function mediated by the EMC lumenal domain.

116 ctromagnetic compatibility and interference (EMC/I) shielding, selective multi-frequency bolometers,

117 infection was unchanged, in cells that lack EMC subunit 4.

118 Cells lacking EMC proteins and the ER-mitochondria tethering complex c

119 ls, and an established epicardial cell line (EMC cells), secrete trophic protein factors into conditi

120 s transplanted with ER-MP20(+) (more mature) EMC than in animals transplanted with unseparated EMC or

121 lanted animals and confirmed that ER-MP20(+) EMC preferentially engrafted.

122 Our data indicate that OEG4-EMC implant is an efficient mode for restoring insulin s

123 rich in transmembrane domains, in absence of EMC.

124 However, the structural basis of EMC assembly and its functions are poorly understood.

125 nate the structural and mechanistic basis of EMC's multifunctionality and point to its role in differ

126 We report here a case of EMC with a t(9;17)(q22;q11.2) as the sole chromosome abn

127 Furthermore, targeted depletion of EMC subunits in live mosquitoes significantly reduced DE

128 Such rapid development of EMC from homozygous loss of endometrial Pten suggests th

129 Partial inhibition of EMC-6 caused decreased expression of heteromeric levamis

130 The likelihood of EMC use in sepsis-related emergencies was 14.9 percentag

131 lts unveiled a novel regulatory mechanism of EMC in HCC progression through lipid metabolism and may

132 RC1 inhibitor, rapamycin, in mouse models of EMC and in human EMC cell lines to explore the interacti

133 translocated by EMC, and constrain models of EMC's proposed chaperone function.

134 polar and stalk cells, and overexpression of EMC caused dramatic egg chamber fusions, indicating that

135 eived urgency was the strongest predictor of EMC use.

136 in detail the initiation and progression of EMC.

137 ics studies beginning to reveal the range of EMC's membrane protein substrates.

138 s away from the main transmembrane region of EMC and is partially mobile.

139 Recently, the function of EMC6, a subunit of EMC, has been attracting attention for its role in cance

140 Treatment of EMC cell lines with Ship1/2 small molecule inhibitors re

141 rthogonally, by serial passaging of virus on EMC-deficient cells, we identified two non-synonymous po

142 ime reversible (GTR) model, used with AWP or EMC, accurately recovered the substitution counts, even

143 rier for Dengue and Zika viruses to overcome EMC depletion.

144 lonyl coenzyme A (ethylmalonyl-CoA) pathway (EMC pathway).

145 n PFS at 6 months in recurrent or persistent EMC and warrants further investigation.

146 Purified EMC in synthetic liposomes catalyzed the insertion of it

147 ith celecoxib and rapamycin markedly reduces EMC progression.

148 nettes, we investigated the decision to seek EMC in sepsis-related compared with other emergencies.

149 sess perceived urgency, the tendency to seek EMC, sepsis knowledge, and barriers to EMC utilization.

150 e perceived urgency and the tendency to seek EMC.

151 al studies distinguish between two separable EMC activities, as an insertase regulating tail-anchored

152 which spontaneously develop CLL, and stable EMC CLL cell lines derived from these mice to explore th

153 Animals challenged with MERS-CoV strain EMC/2012 by the aerosol route responded with primary and

154 the use of this model to specifically study EMC.

155 are currently being used as a model to study EMC.

156 idely conserved multi-protein complex termed EMC has been implicated in membrane protein biogenesis,

157 We demonstrated that EMC was essential for accurate folding and post-translat

158 el are mutually exclusive, and indicate that EMC-to-Ca(V)alpha(2)delta hand-off involves a divalent i

159 been highlighted by reports indicating that EMC dysregulation is linked to neurodegenerative disease

160 ramatic egg chamber fusions, indicating that EMC is a negative regulator of polar and/or stalk cells.

161 e harboring a ZIKV replicon, indicating that EMC participates in the complex process of viral protein

162 We propose that EMC functions downstream of Notch and upstream of EYA to

163 ti-pain and anti-anxiety drugs(6), show that EMC and Ca(V)alpha(2)delta interactions with the channel

164 hemical reconstitution experiments show that EMC can directly mediate the insertion of transmembrane

165 roteins and incoming viral RNA, we show that EMC is required at or prior to virus uncoating.

166 These results suggest that EMC acts between SRP and Sec61 to triage SAs for inserti

167 Our results suggest that EMC's cytosolic domain contains a large, moderately hydr

168 The EMC inserts transmembrane domains (TMDs) with limited hy

169 The EMC structure reveals notable evolutionary conservation

170 ce of distinct entry points for acetate: the EMC pathway and the TCA cycle.

171 unifying model for coordination between the EMC, the multipass translocon, and Sec61 for the biogene

172 Depletion of WNK1 thus destabilizes both the EMC and its membrane protein clients.

173 a non-insertase EMC function mediated by the EMC lumenal domain.

174 Substrate discrimination by the EMC provides a biochemical explanation for one role of c

175 During growth on ethylamine, the EMC pathway operates as a linear pathway carrying the fu

176 olyproteins, which reinforces a role for the EMC in stabilizing challenging transmembrane proteins du

177 ncovered a second and important role for the EMC.

178 r data highlight how flaviviruses hijack the EMC for transmembrane protein biogenesis to achieve opti

179 en together, the present work identifies the EMC complex and VCP in the metabolism of the disease-ass

180 A mutase is a metabolic control point in the EMC pathway, expanding our understanding of its regulati

181 that none are completely independent of the EMC for infection, and that mutant NS4B proteins remain

182 luable proxy to decipher the function of the EMC further.

183 s an aberrant topology in the absence of the EMC leading to degradation.

184 ful condition to study the regulation of the EMC pathway.

185 , we present the co-crystal structure of the EMC that consists of Erh1 homodimers interacting with Mm

186 Disruption of the EMC-Ca(V) complex compromises Ca(V) function, suggesting

187 was a physical and genetic interactor of the EMC.

188 pproach to identify genetic modifiers of the EMC.

189 Functional and structural analysis of the EMC.BOS holocomplex showed that characteristics of a GPC

190 4B and its determinants of dependency on the EMC are different from those previously described in DEN

191 , we demonstrate that this dependency on the EMC for NS4A and NS4B biogenesis extends to the West Nil

192 ferent determinants of its dependency on the EMC than those for DENV NS4B, as well as a different mem

193 structure) are inviable, suggesting that the EMC also functions as a tether.

194 ransfer and cell growth, suggesting that the EMC forms a tether by associating with the TOM complex.

195 promises Ca(V) function, suggesting that the EMC functions as a channel holdase that facilitates chan

196 These results suggest that the EMC is a component of the ER folding machinery.

197 intact in these mutants, suggesting that the EMC performs an additional tethering function to ERMES.

198 Together, our findings support that the EMC tethers ER to mitochondria, which is required for ph

199 We propose that the EMC uses local membrane thinning and a positively charge

200 A cycle and that assimilation occurs via the EMC pathway.

201 ngs are consistent with a model in which the EMC recognizes multiple determinants in the NS4B protein

202 Furthermore, acetyl-CoA condenses with the EMC pathway product glyoxylate, resulting in malate form

203 These EMC subunits also bind to the ER-resident fusion machine

204 Thus, EMC is a transmembrane domain insertase, a function that

205 a pre-insertion SA intermediate adjacent to EMC.

206 letion, suggesting a high genetic barrier to EMC depletion.

207 seek EMC, sepsis knowledge, and barriers to EMC utilization.

208 ceived as less urgent and less often lead to EMC use, even if the level of perceived urgency is the s

209 sia of which approximately 20% progresses to EMC.

210 hat mutant NS4B proteins remain sensitive to EMC depletion, suggesting a high genetic barrier to EMC

211 han in animals transplanted with unseparated EMC or fresh marrow mononuclear cells, a pattern that pe

212 n when situations were rated as very urgent, EMC use was less likely for sepsis-related emergencies (

213 ryoglobulinemia, and none of 6 patients with EMC were positive for HGV RNA, for an overall prevalence