ライフサイエンスコーパス: ExPEC

1 ExPEC causes the vast majority of urinary tract infectio

2 ExPEC harboring a deletion of the gene encoding the mann

3 ExPEC O serotypes are key targets for potential multival

4 ExPEC strains are extracellular bacterial pathogens; the

5 Among 3217 ExPEC blood culture isolates, the most ubiquitous O sero

6 In conclusion, the addition of 38 ExPEC-associated genes and the associated alleles to the

7 In this study, 38 ExPEC-associated virulence genes were added to the exist

8 1 ETEC) (12 associated with TD) and 39 (41%) ExPEC/UPEC (none associated with UTI).

9 of survival was used, four of six additional ExPEC strains, but not an E. coli laboratory strain, wer

10 An effective vaccine against ExPEC infection is urgently needed.

11 trace the fate of PAC after they agglutinate ExPEC and follow PAC-ExPEC complexes in cell culture ass

12 hese 16 O serotypes represented 77.6% of all ExPEC bacteremia isolates analyzed.

13 Increasing multidrug resistance among ExPEC strains constitutes a major obstacle to treatment

14 Predominant O serotypes among ExPEC bacteremia isolates are widespread across differen

15 An ExPEC vaccine effective against strains that are associa

16 Here, we report that FimX, an ExPEC-associated DNA invertase that regulates the major

17 nd 94% of samples, respectively; P<.001) and ExPEC contamination (4%, 19%, and 46%, respectively; P<.

18 ation of antimicrobial-resistant E. coli and ExPEC, which may represent a newly recognized group of m

19 -source E. coli strains classified by PCR as ExPEC and non-ExPEC.

20 Despite more ESBL-PE strains qualifying as ExPEC/UPEC than DEC, travel-acquired ESBL-PE are more of

21 O1 genomic islands among 828 human and avian ExPEC and commensal E. coli isolates was determined.

22 evidence that at least some human and avian ExPEC strains are highly similar to one another, and it

23 dherence phenotype resembling that of canine ExPEC prompted the present reevaluation of the canine-hu

24 These data demonstrate that canine ExPEC strains are similar to, and in some instances esse

25 used to compare the virulence of a clinical ExPEC isolate with its isogenic mutant impaired for the

26 invasive extra-intestinal pathogenic E coli (ExPEC) disease.

27 bored by extraintestinal pathogenic E. coli (ExPEC) and encodes the genotoxin colibactin, is epidemio

28 known as extraintestinal pathogenic E. coli (ExPEC) and include human uropathogenic E. coli (UPEC) an

29 known as extraintestinal pathogenic E. coli (ExPEC) and include pathogens of humans and animals.

30 eages of extraintestinal pathogenic E. coli (ExPEC) and superseded serotyping for certain isolates wi

31 olate of extraintestinal pathogenic E. coli (ExPEC) leads to stable and long-term colonization of the

32 ifferent extraintestinal pathogenic E. coli (ExPEC) phylogroups independently in the last 500 years.

33 classic extraintestinal pathogenic E. coli (ExPEC) sequence types (STs) and case report ST131 househ

34 utant of extraintestinal pathogenic E. coli (ExPEC) strain CFT073 that could not synthesize the K2 ca

35 cialized extraintestinal pathogenic E. coli (ExPEC) strains and, increasingly, with antimicrobial res

36 tions of extraintestinal pathogenic E. coli (ExPEC) was assessed.

37 ified as extraintestinal pathogenic E. coli (ExPEC), and even these isolates exhibited significantly

38 , termed extraintestinal pathogenic E. coli (ExPEC), that have a special ability to cause disease at

39 lones of extraintestinal pathogenic E. coli (ExPEC), which traditionally have been regarded primarily

40 putative extraintestinal pathogenic E. coli (ExPEC)-associated virulence genes.

41 of human extraintestinal pathogenic E. coli (ExPEC).

42 known as extraintestinal pathogenic E. coli (ExPEC).

43 intestinal pathogenic/uropathogenic E. coli (ExPEC/UPEC).

44 xtra-intestinal pathogenic Escherichia coli (ExPEC) belonging to sequence type 95 (ST95) represent a

45 xtra-intestinal pathogenic Escherichia coli (ExPEC) can cause a variety of infections outside of the

46 extraintestinal pathogenic Escherichia coli (ExPEC) encode a variety of fitness and virulence factors

47 xtra-intestinal pathogenic Escherichia coli (ExPEC) in comparison with their propensity to cause bloo

48 Extraintestinal pathogenic Escherichia coli (ExPEC) is an important human and animal pathogen.

49 Extraintestinal pathogenic Escherichia coli (ExPEC) is the leading cause in humans of urinary tract i

50 Extraintestinal pathogenic Escherichia coli (ExPEC) is the leading cause of bacteremia worldwide, wit

51 Extraintestinal pathogenic Escherichia coli (ExPEC) is the most common gram-negative bacterial pathog

52 Extraintestinal pathogenic Escherichia coli (ExPEC) reside in the enteric tract as a commensal reserv

53 Extracellular pathogenic Escherichia coli (ExPEC) strains are common causes of a variety of clinica

54 extraintestinal pathogenic Escherichia coli (ExPEC) strains are incompletely defined.

55 Extraintestinal pathogenic Escherichia coli (ExPEC) strains are typically benign within the mammalian

56 extraintestinal pathogenic Escherichia coli (ExPEC) strains, referred to as uropathogenic E. coli (UP

57 extraintestinal pathogenic Escherichia coli (ExPEC) with infectious potential for humans, presumed ho

58 extraintestinal pathogenic Escherichia coli (ExPEC), 63 environmental canine fecal deposits were eval

59 Extraintestinal Escherichia coli (ExPEC), a heterogeneous group of pathogens, encompasses

60 extraintestinal pathogenic Escherichia coli (ExPEC), a major cause of urinary tract and bloodstream i

61 Extraintestinal pathogenic Escherichia coli (ExPEC), so named because this pathotype infects tissues

62 extraintestinal pathogenic Escherichia coli (ExPEC), such as avian pathogenic E. coli (APEC), and des

63 extraintestinal pathogenic Escherichia coli (ExPEC), which encodes the genotoxin colibactin, are inco

64 xtra-intestinal pathogenic Escherichia coli (ExPEC).

65 the chemotherapeutic drug cyclophosphamide, ExPEC translocates from the intestine to the lungs, live

66 tion-based assays and O serotyping to define ExPEC-associated traits were performed.

67 ibactin synthesis), plus molecularly defined ExPEC status, were significantly associated with virulen

68 for further discrimination of the different ExPEC subpathotypes, serogroups, phylogenetic types, and

69 The food supply may disseminate ExPEC and antimicrobial-resistant E. coli.

70 roles in pathogenesis are well described for ExPEC strains that cause urinary tract infections and me

71 s study indicates that FimH is important for ExPEC translocation, suggesting that the type 1 pilus is

72 intestinal tract is often a prerequisite for ExPEC-mediated pathogenesis, we set out to understand ho

73 Macrophages are required for ExPEC dissemination, suggesting the pathogen has develop

74 d pathogenesis, we set out to understand how ExPEC colonizes this niche.

75 Commonality between canine and human ExPEC has potentially important implications for disease

76 pecific differences between canine and human ExPEC strains have cast doubt on this hypothesis.

77 city-associated island from archetypal human ExPEC strain CFT073.

78 a, or meningitis, including archetypal human ExPEC strains 536, CP9, and RS218.

79 d human isolates, including archetypal human ExPEC strains CFT073 (O6:K2:H1), 536 (O6:K15:H31), and J

80 arison of this genome to all available human ExPEC genomic sequences.

81 the present reevaluation of the canine-human ExPEC connection.

82 es essentially indistinguishable from, human ExPEC strains, which implicates dogs and their feces as

83 re analyzed and compared with those of human ExPEC controls.

84 virulence-associated genes typical of human ExPEC were prevalent among the canine fecal isolates.

85 similarities between APEC O1 and other human ExPEC strains belonging to the ST95 phylogenetic lineage

86 ains were more like APEC O1 than other human ExPEC strains.

87 In addition to pap, human ExPEC-associated virulence genes detected among the cani

88 ping showed that some of the sequenced human ExPEC strains were more like APEC O1 than other human Ex

89 Mutation of either glpG or glpR impaired ExPEC growth in mucus and on plates containing the long-

90 asmids are found significantly more often in ExPEC, including ExPEC associated with human neonatal me

91 significantly more often in ExPEC, including ExPEC associated with human neonatal meningitis and avia

92 PAC inhibit ExPEC invasion of epithelial cells and, therefore, may p

93 associated with pathogenic extra-intestinal (ExPEC) strains.

94 ominant O serotypes associated with invasive ExPEC disease in older adults.

95 Studies using the model ExPEC strain CP9 demonstrated that it is relatively resi

96 source (ie, clinical vs fecal) nor molecular ExPEC status added predictive power to these traits, whi

97 icantly associated with bacteremia, multiple ExPEC-associated virulence genes, and group B2, and with

98 i strains classified by PCR as ExPEC and non-ExPEC.

99 lectively, we have shown that the ability of ExPEC to survive in macrophages is contingent upon the p

100 Characterization of ExPEC strains using various typing techniques has shown

101 rce and established molecular definitions of ExPEC and uropathogenic E. coli (UPEC).

102 rulence in E. coli and that the evolution of ExPEC, which involves extensive horizontal transmission

103 ay for diverse virulence-associated genes of ExPEC.

104 port of a chemotherapy-based animal model of ExPEC translocation in cancerous mice, a system that can

105 deposits were evaluated for the presence of ExPEC by a combination of selective culturing, extended

106 bution and antibiotic resistance profiles of ExPEC strains causing bloodstream infections across 4 re

107 hich consequently may provide a reservoir of ExPEC for acquisition by humans.

108 The present study examined a subset of ExPEC strains: neonatal meningitis E. coli (NMEC) strain

109 These findings suggest that survival of ExPEC within neutrophils may be an important virulence m

110 Translocation of ExPEC is also associated with an increase of the diversi

111 des a further comprehensive understanding of ExPEC-related virulence, host specificity, and adaptatio

112 he extraintestinal pathogenic versatility of ExPEC clones, which supports the use of an inclusive des

113 flicting results related to the virulence of ExPEC strains.

114 o be crucial to the fitness and virulence of ExPEC.

115 side (extraintestinal pathogenic E. coli, or ExPEC).

116 Regardless of host of origin, ExPEC strains share many traits.

117 ld help us understand the virulence of other ExPEC strains and design more efficient infection contro

118 after they agglutinate ExPEC and follow PAC-ExPEC complexes in cell culture assays.

119 ived isolates of extraintestinal pathogenic (ExPEC) Escherichia coli, a common agent of sepsis and co

120 at includes many extraintestinal pathogenic (ExPEC) strains.

121 Fluorescent labeled PAC were able to promote ExPEC agglutination when observed with fluorescence micr

122 l pks island markers), and 12 other putative ExPEC virulence genes were newly sought by PCR among 131

123 n of glpG but not glpR significantly reduced ExPEC survival.

124 PEC O1's genome not found in other sequenced ExPEC genomes.

125 es in their known virulence attributes, some ExPEC strains can cross the host species barrier and pre

126 mechanisms underlie host specificity in some ExPEC strains.

127 that might contribute to the ability of some ExPEC strains to cause high-level bacteremia and meningi

128 Four food-source ExPEC isolates (from pea pods, turkey parts, ground pork

129 lower virulence scores than did susceptible ExPEC (7.25 vs. 9.0; P=.001).

130 In contrast, multiple known or suspected ExPEC virulence genes, including pap (P fimbriae), vat (

131 These data confirm that ExPEC representing known virulent clones are highly prev

132 We find that ExPEC E. coli are highly genomically heterogeneous, cons

133 host species, leading to the hypothesis that ExPEC may have zoonotic potential.

134 and transformed Caco-2 cells, we report that ExPEC strain CP9 binds to and invades the intestinal epi

135 nal frameshifting and profoundly altered the ExPEC proteome, with variable effects attributable to UN

136 uencing (TraDIS) strategy to investigate the ExPEC XM strain, which is capable of crossing the host s

137 e lines of evidence suggest that many of the ExPEC strains encountered in humans with urinary tract i

138 Multiple genes that contribute to ExPEC fitness in mucus broth were identified, with genes

139 rther validation, and their contributions to ExPEC virulence in both mammalian and avian models or ma

140 ontains 139 genes-of which 44 are related to ExPEC-and 2,826 corresponding alleles.

141 model of chemotherapy-induced translocation, ExPEC lacking fimH colonized at levels comparable to tha

142 rther challenges for preventing and treating ExPEC infections.

143 mutant, and oxyRS mutants of other wild-type ExPEC strains, exhibited significantly increased in vitr

144 rred either in APEC O1 or in highly virulent ExPEC isolates, resulting in differences in pathogenicit

145 ction were also identified in other virulent ExPEC STs, Shigella and Salmonella, suggesting a correla

146 Of ESBL-PE, 3 (3%) were ExPEC/UPEC-EAEC hybrids (2 associated with diarrhea, non

147 new avenues for defining mechanisms by which ExPEC strains colonize the mammalian gastrointestinal tr

148 oxidative burst is not a mechanism by which ExPEC survives within neutrophils.

149 increasing healthcare costs associated with ExPEC infections.

150 everal virulence factors are associated with ExPEC, there is no core set of virulence factors that ca

151 eq) was performed to search for genes within ExPEC isolate F11 that are important for growth in intes