ライフサイエンスコーパス: F. nucleatum

1 F. nucleatum activated p38 and c-Jun NH(2)-terminal kina

2 F. nucleatum activates IL-1beta processing through the N

3 F. nucleatum also induces the mobilization of immune cel

4 F. nucleatum also promoted invasion of KB cells by other

5 F. nucleatum and C. rectus were also associated with EOP

6 F. nucleatum and S. cristatus coaggregate strongly via a

7 F. nucleatum binding to clinical adenocarcinomas correla

8 F. nucleatum biofilm coculture with OD-E model causes la

9 F. nucleatum cell wall and FAD-I induced hBD2 via TLR2.

10 F. nucleatum isolated from other parts of the body may o

11 F. nucleatum ssp nucleatum, ssp polymorphum and P. gingi

12 F. nucleatum ssp polymorphum caused considerable mortali

13 F. nucleatum ssp. nucleatum and ssp. polymorphum signifi

14 F. nucleatum strains isolated from amniotic fluids and p

15 F. nucleatum synthesizes lanthionine for its peptidoglyc

16 F. nucleatum treatment induced apoptosis of PBMCs and PM

17 F. nucleatum was first detected in the blood vessels in

18 F. nucleatum was systematically detected in samples with

19 tabase further correctly identified 28 of 34 F. nucleatum clinical isolates to the subspecies level.

20 either Actinobacillus actinomycetemcomitans, F. nucleatum, or P. gingivalis.

21 In addition, F. nucleatum seems to be important for the development o

22 he IL-8 secreted from epithelial cells after F. nucleatum stimulation could be down-regulated by subs

23 All F. nucleatum strains stimulated significant increase in

24 trophils were significantly increased in all F. nucleatum groups compared with control (P <0.001).

25 Although F. nucleatum lacks canonical RNA chaperones, such as Hfq

26 Although F. nucleatum vincentii also reduced superoxide generatio

27 Although F. nucleatum vincentii also reduced superoxide generatio

28 mcomitans exhibited mutualism, and, although F. nucleatum was unable to grow with either of the other

29 It is highly conserved among F. nucleatum, Fusobacterium periodonticum, and Fusobacte

30 tial role for this enzyme in establishing an F. nucleatum intracellular niche.

31 ort the isolation and characterization of an F. nucleatum (ATCC 25586)-associated defensin inducer (F

32 we present the first characterization of an F. nucleatum Type Vd phospholipase class A1 autotranspor

33 colonization of A. actinomycetemcomitans and F. nucleatum/periodonticum was not statistically associa

34 P. gingivalis, A. actinomycetemcomitans, and F. nucleatum was found to be higher in healthy individua

35 rs, Gardnerella vaginalis, S. agalactiae and F. nucleatum to vaginal epithelial cells is partially me

36 physical interaction between C. albicans and F. nucleatum and for the first time revealed the identit

37 that the interaction between C. albicans and F. nucleatum leads to a mutual attenuation of virulence,

38 physical interaction between C. albicans and F. nucleatum was mediated by the carbohydrate components

39 the regulation of hBD-2 mRNA; TNF-alpha and F. nucleatum cell wall induced hBD-2 mRNA rapidly (2 to

40 to the Bayesian bivariate LCM, S. bovis and F. nucleatum had a more significant predictive accuracy

41 iate LCMs, the sensitivities of S. bovis and F. nucleatum were calculated as 93% (95% CrI 0.84-0.98)

42 rated that the sensitivities of S. bovis and F. nucleatum were estimated to be 86% [95% credible inte

43 Bacterial species including S. bovis and F. nucleatum were measured by absolute quantitative real

44 CrI 0.73-0.85) respectively for S. bovis and F. nucleatum.

45 etected in the matching AF, with E. coli and F. nucleatum as the most prevalent.

46 capable of transforming Escherichia coli and F. nucleatum ATCC 10953 was constructed with pFN1.

47 ccharides isolated from Escherichia coli and F. nucleatum were poor stimulants of hBD-2, although the

48 In both E. coli and F. nucleatum, FadA exists in two forms, the intact pre-F

49 stainings were negative in both controls and F. nucleatum cocultures.

50 A. actinomycetemcomitans, E. corrodens, and F. nucleatum was determined using an immunoassay.

51 sequential challenge with P. gingivalis and F. nucleatum and vice versa were approximately identical

52 otential, and consortia of P. gingivalis and F. nucleatum are synergistically pathogenic within in vi

53 periodontitis induced by a P. gingivalis and F. nucleatum mixed infection, and also on the local host

54 Infection of mice with P. gingivalis and F. nucleatum strains elicited lesions of various sizes a

55 Increased levels of P. gingivalis and F. nucleatum were associated with periodontitis in both

56 -10 after incubations with P. gingivalis and F. nucleatum, as well as significantly reduced the expre

57 hages that were exposed to P. gingivalis and F. nucleatum, without impairing their viability.

58 with/without priming with P. gingivalis and F. nucleatum.

59 S. mutans, S. sanguis, P. gingivalis, and F. nucleatum were incubated with serial dilutions (1/4,

60 and F. nucleatum but not with S. oralis and F. nucleatum, indicating that P. gingivalis and S. orali

61 The aggregation of PBMCs and F. nucleatum T18 was inhibited by either L-arginine, L-l

62 for example, Fusobacterium periodonticum and F. nucleatum) and donor abundance.

63 Also, it grew with Veillonella sp. and F. nucleatum but not with S. oralis and F. nucleatum, in

64 ygen requirements (E. coli, A. viscosus, and F. nucleatum), as well as a model mammalian cell line (m

65 -P. gingivalis, anti-P. intermedia, and anti-F. nucleatum antibody concentrations displayed significa

66 001 and p = 0.020, respectively), while anti-F. nucleatum was positively associated with IgG anti-MAA

67 We suggest that A. aphrophilus, F. nucleatum, and S. intermedius are key pathogens for t

68 These miRNAs can enter bacteria, such as F. nucleatum and E. coli, specifically regulate bacteria

69 At F. nucleatum/P. gingivalis ratios of > or = 1:1, spreadi

70 Coaggregation-mediated interactions between F. nucleatum and other species facilitated the survival

71 tants, we investigated the interplay between F. nucleatum outer membrane protein RadD and different S

72 The CoAg reaction between F. nucleatum and the Candida species involved a heat-lab

73 The CoAg reactions between F. nucleatum and the Candida species may be important in

74 Moreover, anti-FAD-I antibodies blocked F. nucleatum induction of hBD-2 by more than 80%.

75 l cavity, and the CoAg of C. dubliniensis by F. nucleatum when grown at 37 degrees C provides a rapid

76 utral and sialic acid-terminating glycans by F. nucleatum.

77 iological pathways significantly impacted by F. nucleatum and S. gordonii included the mitogen-activa

78 f HDAC1 recapitulated the effects induced by F. nucleatum or butyrate.

79 he expression of interferon-gamma induced by F. nucleatum.

80 However, hBD-2 induction by F. nucleatum was not blocked by pretreatment with two NF

81 NK partially blocked hBD-2 mRNA induction by F. nucleatum, and the combination of two inhibitors comp

82 hat T cell activities were also inhibited by F. nucleatum via Fap2.

83 BD-2 induction, and that hBD-2 regulation by F. nucleatum is via p38 and JNK, while phorbol ester ind

84 ls of CCL20 at 6 h, following stimulation by F. nucleatum cell wall (FnCW).

85 MCPIP-1 protein expression was suppressed by F. nucleatum and MALT-1 protein expression was suppresse

86 MALT-1 protein expression was suppressed by F. nucleatum, P. gingivalis LPS and IL-1beta.

87 helial cell, as well as protein synthesis by F. nucleatum.

88 her bacteria linked to colorectal carcinoma, F. nucleatum does not exacerbate colitis, enteritis, or

89 Interestingly, in flow cells F. nucleatum and A. actinomycetemcomitans exhibited mutu

90 a-, or IL-10-producing cells after challenge F. nucleatum.

91 In this study, a fadA-complementing clone, F. nucleatum 12230 USF81, was constructed.

92 By contrast, F. nucleatum strains contain genomic expansions of Type

93 In contrast, F. nucleatum was able to coaggregate not only with both

94 While controlling F. nucleatum through antibiotics could reduce cancer sev

95 igher levels of P. intermedia, E. corrodens, F. nucleatum, and IL-1beta than non-users.

96 is designed to determine impact of different F. nucleatum strains on neutrophil function.

97 culture in the presence/absence of different F. nucleatum strains.

98 The invasive ability may enable F. nucleatum to colonize and infect the pregnant uterus.

99 accessible because of the lack of endogenous F. nucleatum sialidase.

100 In the oral environment, F. nucleatum adheres to a large diversity of species, fa

101 Given its prevalence in EONS, F. nucleatum should be placed on the same importance sca

102 ological research has now firmly established F. nucleatum as an oncomicrobe associated with several m

103 In addition to FadA, F. nucleatum ATCC 25586 and ATCC 49256 also encode two p

104 MALDI-TOF MS spectra of five F. nucleatum subspecies (animalis, fusiforme, nucleatum,

105 dentical and were lower than those following F. nucleatum challenge alone and higher than control lev

106 eficient mice (C57BL/6 TLR2(-/-)), following F. nucleatum infection.

107 11% for Porphyromonas gingivalis to 40% for F. nucleatum.

108 may be an important virulence mechanism for F. nucleatum to infect the placenta.

109 endothelial receptor for FadA, required for F. nucleatum binding to the cells.

110 resent the first complementation studies for F. nucleatum.

111 developing diagnostics and therapeutics for F. nucleatum.

112 lecular analyses demonstrated that cell-free F. nucleatum membranes are sufficient to induce cell dea

113 ious studies identified a novel adhesin from F. nucleatum, FadA, as being involved in the attachment

114 those of native FAD-I (nFAD-I) isolated from F. nucleatum ATCC 25586.

115 Conditioned medium from F. nucleatum-infected HCT116 cells caused naive cells to

116 select subtype(s) of a given species, e.g., F. nucleatum subspecies animalis and polymorphum and S.

117 gingivalis, F. nucleatum, S. sanguis, and A. naeslundii were grown o

118 mRNA levels were increased by P. gingivalis, F. nucleatum, and IL-1beta, however, no changes were obs

119 Although a trend for higher F. nucleatum and P. gingivalis concentrations in aCCP-po

120 We discuss how F. nucleatum potentially uses this lactate utilization g

121 r caspase-3 was expressed in controls and in F. nucleatum laboratory strain ATCC cocultures throughou

122 rginine-inhibitable adhesin-encoding gene in F. nucleatum that is involved in interspecies coadherenc

123 Knockout of hisR in F. nucleatum results in a small but reproducible lag in

124 The role of TNF-alpha as an intermediary in F. nucleatum signaling was ruled out by addition of anti

125 routing into the connective tissue matrix in F. nucleatum OD-E cocultures.

126 of the outer membrane family of proteins in F. nucleatum.

127 ns, little is known about gene regulation in F. nucleatum itself, including global stress-response pa

128 racterization of a global stress response in F. nucleatum, the genetic tools developed here will enab

129 Thus, FadA plays an important role in F. nucleatum colonization in vivo.

130 o studies to determine its potential role in F. nucleatum pathogenesis.

131 ence of a restriction-modification system in F. nucleatum.

132 the cells attached to the tooth, whereas in F. nucleatum biofilm-treated cultures, the Ki-67-express

133 ular migration were attenuated by inhibiting F. nucleatum host-cell binding and entry using galactose

134 t this hypothesis, we intravenously injected F. nucleatum into pregnant CF-1 mice.

135 Additionally, intravenously injected F. nucleatum localizes to mouse tumor tissues in a Fap2-

136 no-mupirocin was active against intratumoral F. nucleatum, a tumor promoting bacteria that accumulate

137 ent of S. cristatus to adherent and invading F. nucleatum.

138 heroid microenvironment, whereas heat-killed F. nucleatum is internalized and sequestered in the canc

139 tum was augmented compared with that of live F. nucleatum.

140 ed, in part due to challenges in maintaining F. nucleatum viability under standard aerobic cell cultu

141 Mechanistically, F. nucleatum targeted TLR4 and MYD88 innate immune signa

142 emcomitans, A. viscosus, B. melaninogenicus, F. nucleatum, P. gingivalis, P. intermedia, S. mutans, S

143 The fadA deletion mutant F. nucleatum 12230 US1 was defective in host cell attach

144 lidase-producing vaginal microbiotas, mutant F. nucleatum unable to consume sialic acids was impaired

145 A double-crossover fadA deletion mutant, F. nucleatum 12230-US1, was constructed by utilizing a n

146 The presence of Fusobacterium nucleatum (F. nucleatum) and Bacteroides fragilis (B. fragilis) in

147 erformance of fecal Fusobacterium nucleatum (F. nucleatum) and Streptococcus bovis (S. bovis) for tim

148 Effects of Fusobacterium nucleatum (F. nucleatum) biofilm on epithelial cell proliferation,

149 n (Anaerobe Helsinki Negative [AHN] 9508) of F. nucleatum was placed on the top of the model.

150 The ability of F. nucleatum 12230, US1, and USF81 to colonize the mouse

151 The ability of F. nucleatum to induce apoptosis was abolished by either

152 The ability of F. nucleatum to inhibit mononuclear cell proliferation m

153 The effects of the presence or absence of F. nucleatum on anaerobe survival in both the biofilm an

154 In an equivalent culture in the absence of F. nucleatum, the numbers of black-pigmented anaerobes (

155 nsible for arginine-inhibitable adherence of F. nucleatum and provides definitive molecular evidence

156 scriptional level, and the administration of F. nucleatum or butyrate enhanced NVDR by increasing DAT

157 attachment loss >2 mm, whereas the amount of F. nucleatum DNA did.

158 epidemiological evidence for association of F. nucleatum and P. aeruginosa with OSCC.

159 eractions were involved in the attachment of F. nucleatum to KB cells.

160 ractions are also involved in the binding of F. nucleatum to host cells.

161 The metabolic capabilities of F. nucleatum revealed by its genome are therefore consis

162 were aggregated in the presence of cells of F. nucleatum but not control bacteria.

163 e attachment and invasion characteristics of F. nucleatum were also tested using KB cells, an oral ep

164 present study, we showed that coinfection of F. nucleatum and T. forsythia is more potent than infect

165 ural space infection may be a combination of F. nucleatum group and/or S. intermedius, with or withou

166 a provide further evidence on the effects of F. nucleatum on endothelium adhesion molecule abundance

167 were isolated from the membrane fraction of F. nucleatum ATCC 23726 and identified via mass spectros

168 biosynthetic pathway for DAP, the genome of F. nucleatum ATCC 25586 encodes a predicted DAP epimeras

169 Co-incubation of F. nucleatum and Escherichia coli enhanced penetration o

170 Inhibition of F. nucleatum host cell attachment and invasion with gala

171 A spontaneous mutant, lam, of F. nucleatum, isolated as defective in autoagglutination

172 A DNA library of F. nucleatum FDC 364 was constructed by partial digestio

173 Using a library of F. nucleatum mutants, we found that the Fap2 protein of

174 Possible mechanisms of F. nucleatum's role in the pathogenesis of periodontal d

175 t was significantly lower than the number of F. nucleatum-positive subjects around teeth (P < 0.05).

176 The number of F. nucleatum-positive subjects around the implant was si

177 ERT-2 epithelial cells with equal numbers of F. nucleatum and S. cristatus bacteria led to significan

178 albicans SN152 mutant library and a panel of F. nucleatum 23726 outer membrane protein mutants, we id

179 ortant for understanding the pathogenesis of F. nucleatum.

180 Although the phagocytosis pattern of F. nucleatum in the mixed infection remained similar to

181 Neutrophil phagocytosis of F. nucleatum ssp. polymorphum was significantly greater

182 Neutrophil phagocytosis of F. nucleatum ssp. polymorphum was significantly greater

183 Neutrophil phagocytosis of F. nucleatum strains and neutrophil apoptosis were analy

184 cells may reflect the invasive phenotype of F. nucleatum and contribute to the greater pathogenic po

185 ibute to the greater pathogenic potential of F. nucleatum than of S. gordonii.

186 In the presence of F. nucleatum, anaerobes persisted in high numbers (>10(7

187 sis of prevalence studies, the prevalence of F. nucleatum among 19 countries and B. fragilis among 10

188 In this study, the prevalence of F. nucleatum and B. fragilis among CRC patients has been

189 ur results emphasized the high prevalence of F. nucleatum and B. fragilis in CRC patients.

190 aled that Asia had the highest prevalence of F. nucleatum while most of the B. fragilis isolates in C

191 m mutants, we found that the Fap2 protein of F. nucleatum directly interacted with TIGIT, leading to

192 in which tumors exploit the Fap2 protein of F. nucleatum to inhibit immune cell activity via TIGIT.

193 tokines and CCL20 suggests the broad role of F. nucleatum and human antimicrobial peptides in primary

194 uld improve our understanding of the role of F. nucleatum in periodontal infections.

195 indicate that the immunosuppressive role of F. nucleatum is largely due to the ability of this organ

196 lated bone resorption and that the strain of F. nucleatum used appeared to be the strongest inducer o

197 e findings suggest that different strains of F. nucleatum impact neutrophil function in different way

198 All strains of F. nucleatum significantly increased phagocytic capacity

199 with human and cynomolgus monkey strains of F. nucleatum.

200 l induction of hBD-2 by different strains of F. nucleatum; ATCC 25586 and ATCC 23726 induce significa

201 rphum was significantly greater than that of F. nucleatum ssp. polymorphum significantly blocked fMLP

202 rphum was significantly greater than that of F. nucleatum ssp. vincentii and ssp. nucleatum (P <0.001

203 Given the ubiquity of F. nucleatum in the human mouth, these studies also sugg

204 obial infection underscored the virulence of F. nucleatum ssp polymorphum in particular with increase

205 path for anaerobic heme catabolism, offering F. nucleatum a competitive advantage in the colonization

206 species involved a heat-labile component on F. nucleatum and a mannan-containing heat-stable recepto

207 reappraisal of fusobacteria with a focus on F. nucleatum as a mutualist, infectious agent and oncoge

208 ached P. gingivalis but had no influences on F. nucleatum bacterial adherence.

209 valis simultaneously (at different sites) or F. nucleatum administered within 4 h prior to or 1 h fol

210 Overall, F. nucleatum and S. gordonii perturbed the gingival epit

211 he presence of any orange-complex pathogens (F. nucleatum, P. intermedia, and C. rectus), total cance

212 A may be a therapeutic target for preventing F. nucleatum colonization of the host.

213 ains, indicating that FAD-I is the principal F. nucleatum agent for hBD-2 induction in HOECs.

214 tivity, a diagnostic feature of BV, promoted F. nucleatum foraging and growth on mammalian sialoglyca

215 The purified F. nucleatum immunosuppressive protein (FIP) is composed

216 0.05), but no antibiotic was able to reduce F. nucleatum.

217 could be an effective strategy for reducing F. nucleatum-associated CRC metastasis.

218 ore, the yeast form of C. albicans repressed F. nucleatum-induced MCP-1 and TNFalpha production in ma

219 Casasanta et al show that CRC cell-resident F. nucleatum promotes cytokine secretion that may potent

220 Hemagglutination of some F. nucleatum strains is also galactose sensitive, sugges

221 Strikingly, F. nucleatum achieves virulence in the absence of large,

222 In this study, F. nucleatum was shown to activate both TLR2 and TLR4 in

223 e latter in the transwell assays, suggesting F. nucleatum may serve as an 'enabler' for other microor

224 ther of the other species in the peg system, F. nucleatum stimulated the growth of Veillonella sp. an

225 Measuring and targeting F. nucleatum and its associated pathway will yield valua

226 Thus, targeting F. nucleatum Fap2 or host epithelial Gal-GalNAc may redu

227 Recently, we demonstrated that F. nucleatum ATCC 23726 coaggregates with C. albicans SN

228 erleukin-6 (IL-6) and IL-8 demonstrated that F. nucleatum induced production of these cytokines, wher

229 tic and functional studies demonstrated that F. nucleatum promoted colorectal cancer resistance to ch

230 udies provided an initial demonstration that F. nucleatum adhered to and invaded HGEC and that this w

231 ments in mice also led to the discovery that F. nucleatum may also "give back" to the community by re

232 provides definitive molecular evidence that F. nucleatum adhesins play a vital role in inter-species

233 his study represents the first evidence that F. nucleatum may be transmitted hematogenously to the pl

234 We found that F. nucleatum ATCC 23726 inhibits growth and hyphal morph

235 We found that F. nucleatum infection induced both normal pancreatic ep

236 erless gene deletion approach and found that F. nucleatum invaded cultured HCT116 CRC cells through t

237 These studies indicate that F. nucleatum aggregates PBMCs, and that this interaction

238 Data indicate that F. nucleatum impaired endothelial cell proliferation and

239 These studies indicate that F. nucleatum may facilitate the colonization of epitheli

240 The findings indicate that F. nucleatum supports AUD through epigenetic regulation

241 The data also indicated that F. nucleatum-induced cell apoptosis requires activation

242 tic analysis of sequence data indicates that F. nucleatum, F. necrophorum, and F. varium are the spec

243 We have shown previously that F. nucleatum adheres to and invades host epithelial and

244 Thus, our findings show that F. nucleatum both directly and indirectly modulates immu

245 However, inhibitor studies show that F. nucleatum stimulation of hBD-2 mRNA requires both new

246 These data show that F. nucleatum subspecies and T. forsythia synergistically

247 vaginal bacterial communities, we show that F. nucleatum supported robust outgrowth of Gardnerella v

248 Previously, it was shown that F. nucleatum induced preterm and term stillbirth in mice

249 Previous studies have shown that F. nucleatum species synergize with T. forsythia during

250 The F. nucleatum strain ATCC 25586 genome was assembled from

251 pHS17 was stably maintained in the F. nucleatum transformants, and differences in the trans

252 ine macrophage cell line, we showed that the F. nucleatum-induced inhibition of Candida hyphal morpho

253 erminal sequences of these proteins with the F. nucleatum genome revealed that the genes encoding the

254 Our data support that this F. nucleatum-mediated inhibition is mediated by human, b

255 Thus, F. nucleatum infection in the pancreas elicits cytokine

256 Thus, F. nucleatum orchestrates a molecular network of the Tol

257 0018), to P. gingivalis (P = 0.0013), and to F. nucleatum (P = 0.0200) than women who delivered at te

258 In addition, polyclonal antibodies to F. nucleatum, which inhibited fusobacterial attachment t

259 Tumors from Apc(Min/+) mice exposed to F. nucleatum exhibit a proinflammatory expression signat

260 lecular level, the genetically transformable F. nucleatum strain ATCC 23726 was screened for adherenc

261 bled more than 70% long-term survival in two F. nucleatum-infected CRC models.

262 t the strong coaggregation between wild-type F. nucleatum 23726 and C. albicans SN152 in an in vitro

263 mors is inhibited in the presence of various F. nucleatum strains.

264 We observed that viable F. nucleatum assembles biofilm-like structures in the tu

265 When F. nucleatum T18 was incubated with PHA-stimulated PBMCs

266 r the 6-month period in both groups, whereas F. nucleatum was significantly reduced in all visits in

267 release of bacterial-derived butyrate, while F. nucleatum and CRC cells are eliminated.

268 This may explain why F. nucleatum is often found in mixed infections.

269 d C. albicans strains demonstrated CoAg with F. nucleatum.

270 ns grown at 37 degrees C to coaggregate with F. nucleatum.

271 ly C. dubliniensis strains coaggregated with F. nucleatum ATCC 49256 and no C. albicans strains showe

272 truction either alone or in combination with F. nucleatum.

273 were strong TLR4 agonists when combined with F. nucleatum LPS.

274 into neutrophil-like cells and cultured with F. nucleatum strains of subspecies (ssp.) nucleatum ATCC

275 incentii (FNV), and compare that genome with F. nucleatum ATCC 25586 (FN).

276 Infection with F. nucleatum and P. gingivalis simultaneously (at differ

277 hort, high polygenic risk and infection with F. nucleatum have a small, yet independent impact on CHD

278 Primary infection with F. nucleatum plus P. gingivalis at various ratios (i.e.,

279 116 cells was increased after infection with F. nucleatum; however, no species significantly altered

280 of gnotobiotic Drosophila melanogaster with F. nucleatum or supplementing the flies' diet with the S

281 an gingival epithelial cells stimulated with F. nucleatum cell wall extract, indicating possible invo

282 by immunofluorescence in HGE stimulated with F. nucleatum cell wall, consistent with induction of the

283 helium, in contrast to cultures treated with F. nucleatum clinical strain AHN, in which caspase-3 was

284 the polymicrobial consortium with or without F. nucleatum exhibited significantly increased alveolar