ライフサイエンスコーパス: FCS

1 FCS analysis of a green fluorescent protein (GFP)-tagged

2 FCS analysis of CA200645-occupied A3ARs revealed 2 speci

3 FCS and PCH analysis of fluorescence-tagged 5-HT(2C) rec

4 FCS and temporally resolved genetic studies uncovered th

5 FCS measurements from solution mixtures of dye-labeled p

6 FCS measurements indicate that pH-dependent formation of

7 FCS measurements revealed hindered diffusion of lipids i

8 FCS measurements show that PIP(2) diffuses rapidly (D ~

9 FCS measures the spatial and temporal correlation of ind

10 FCS provided plasma membrane diffusion coefficients on t

11 FCS was used to record fluctuations in fluorescence inte

12 FCS with PCH revealed molecular brightness values for na

13 concentrations with proper serum levels (10% FCS) and growth factor combinations (EGF, NGF) yielded t

14 ntation induced by Spider media and YPD, 10% FCS, and biofilm formation and macrophage killing were u

15 with denaturant concentration obtained by 2f-FCS and DLS.

16 us fluorescence correlation spectroscopy (2f-FCS) to characterize the denaturant dependence of the un

17 In recent years, although FCS diffusion law analysis has been instrumental in prov

18 Analyzing FCS data with suitable kinetic model we obtain transient

19 t groups after exposure to dexamethasone and FCS, and these were associated with biological pathways

20 ASMCs were exposed to dexamethasone and FCS.

21 Manually obtained measurements of FCP and FCS were also compared.

22 alyze the SMT data and to guide the FRAP and FCS analysis, we show how all three approaches yield sim

23 easure the diffusion coefficient by FRAP and FCS in the exact same images.

24 When this effect is minimized, FRAP and FCS measurements nearly agree, although cross-validation

25 n concentrations typically used for FRAP and FCS, nor is it likely due to spatial heterogeneity of th

26 ng molecular interpretations to the FRET and FCS experiments.

27 s also shown how the combination of SICS and FCS can be used to determine the fraction of fluorescent

28 remains challenging with currently available FCS methods.

29 g droplets for noise reduction by averaging, FCS can monitor accurately the droplets flow even if the

30 parameters on the capability of camera-based FCS to determine membrane heterogeneity via the FCS diff

31 ficient experimental design for camera-based FCS to extract information on mobility, concentration, a

32 d concentration measurements in camera-based FCS.

33 During the working-memory experiment, both FCS-rCBF coupling and rCBF/FCS ratio were modulated by t

34 al Gaussian geometry ubiquitously assumed by FCS theory, or when properties of multiple fluorescent s

35 peptides reach the cytosol, as determined by FCS, correlates directly with their efficacy in cell-bas

36 ses without denaturing agent as evidenced by FCS and gel-based multimer analysis.

37 binding to IL8 and IL6 promoters induced by FCS+TGF-beta.

38 RAP is 15 times longer than that obtained by FCS.

39 By analyzing live-cell FCS measurements, we show that our new model can satisfa

40 Combining FCS, DLS, and TEM results, we find three distinct period

41 By combining FCS and video-microscopy (with PTV and PIV processing ap

42 Overall, combining FCS, PIV, and PTV analysis of redox-MHD is a powerful co

43 Combining comprehensive FCS controls, ABEL trap, surface-based single-molecule f

44 is method with stimulated emission depletion FCS for performing FCS at subdiffraction spatial scales.

45 or manually derived FCP and manually derived FCS thickness was 0.94 (P < 0.001), with a smaller mean

46 esting multiple competing models to describe FCS data based on temporal autocorrelation functions.

47 Our finite-domain FCS predicts simulated data accurately and reduces to a

48 ctions introduced by the DNA binding domain, FCS generates independent estimates for the diffusion co

49 Here, we apply this procedure to evaluate FCS data from fluorescent proteins assayed in vitro and

50 en aggregation and toxicity are exacerbated, FCS-based burst analysis and purified single molecule FC

51 ons support the analysis of the experimental FCS data.

52 es, we integrate our model with experimental FCS data to capture the nascent protein statistics and t

53 Hence, extending FCS for periodically passing objects converts it into a

54 mented resonant line-scan STED with filtered FCS, which has the additional benefit of autocalibrating

55 nd fractions, or even fail altogether to fit FCS binding data.

56 he necessity of a photobleach correction for FCS measurements of GFP-tagged molecules that are bound

57 a new and potentially interesting field for FCS studies could be the study of nonequilibrium steady

58 of competing, non-nested physical models for FCS data, appropriately penalizing model complexity acco

59 ed these nanoparticles using ITC, DLS, FRET, FCS, TIRF, and TEM.

60 FRET-FCS and sm-FRET measurements are comparable and show tha

61 FRET-FCS could readily detect a FRET-active oligonucleotide p

62 When applied to the analysis of Abeta, FRET-FCS detected oligomers consisting of less than 10 Abeta

63 distances is relatively narrow for both FRET-FCS and sm-FRET, suggesting that the two packaged DNA en

64 This ability of FRET-FCS could be an indispensable tool for studying biologic

65 Fluorescence Correlation Spectroscopy (FRET-FCS) has a unique ability to detect small subpopulations

66 From FCS autocorrelation functions, we obtain the number of f

67 ical mechanistic insight is often drawn from FCS experiments by fitting the resulting time-intensity

68 ion of model parameter values estimated from FCS data.

69 roach to directly measure binding rates from FCS data.

70 On the other hand, FCS studies show an unexpected increase the mobility of

71 ver, based on the principles described here, FCS can be straightforwardly applied for a variety of sy

72 ing state, functional brain hubs with higher FCS were identified, primarily in the default-mode, insu

73 However, FCS data analysis and interpretation using fluorescent p

74 addition to its practical utility, however, FCS provides a window on mesoscopic systems in which flu

75 With the advent of camera-based imaging FCS, which measures the diffusion coefficient in each pi

76 biased model-based interpretation of imaging FCS data, with broad applicability to resolving the hete

77 ional analysis and interpretation of imaging FCS datasets.

78 commonly accepted interpretations of imaging FCS diffusion law analysis, and we show that exceptions

79 As proof of concept, imaging-FCS was used to measure surface diffusion rates, interfa

80 combination of these two techniques, imaging-FCS, for measurement of fast interfacial transport at a

81 ds, specified within the multiple imputation FCS method, for selected predictors for each operation u

82 In FCS experiments, we found that the catch bond character

83 y assess the latent information contained in FCS datasets.

84 The mean error in FCS retinal thickness measurement increased as the densi

85 By using simple modifications in FCS setup, we describe how one can extract the reaction

86 te that photobleaching of bound molecules in FCS is mainly responsible.

87 e highly correlated noise that is present in FCS data sets and additionally penalizes model complexit

88 r the apparent diffusion enhancement seen in FCS.

89 on can acutely lower plasma triglycerides in FCS.

90 o be analyzed by existing methods, including FCS.

91 JQ1/SGCBD01 and I-BET762 inhibited FCS+TGF-beta-induced ASM cell proliferation and IL-6 and

92 f the diffusion coefficient explored by ITIR-FCS together with MD simulations yields insights into Lt

93 We perform ITIR-FCS measurements on supported lipid bilayers (SLBs) of v

94 fluorescence correlation spectroscopy (ITIR-FCS) and molecular dynamics (MD) simulations.

95 fluorescence correlation spectroscopy (ITIR-FCS) is a well-established mobility-based method that pr

96 -fluorescence correlation spectroscopy (ITIR-FCS) showed that monomeric hIAPP induced the formation o

97 eveloped Bayesian analysis procedure to ITIR-FCS data to resolve hIAPP-induced microdomain spatial or

98 (AFM) with the dynamics measured using ITIR-FCS.

99 useful in larger-scale modeling of kinetics, FCS, and FRAP.

100 ential for use as a therapeutic for managing FCS.

101 les passively moving in a homogeneous media, FCS analysis yields analytical functions that can be fit

102 nemia syndrome cases are the result of MFCS; FCS is very rare.

103 ly implemented on a fluorescence microscope, FCS samples femtoliter volumes and so is especially usef

104 burst analysis and purified single molecule FCS detected a populational shift toward an increase in

105 Single-molecule FCS is a useful procedure for measuring dissociation equ

106 n fluorescence correlation spectroscopy (MPE-FCS).

107 We apply this new FCS method to evaluate the efficacy of a potential antic

108 culture supernatant fluid in the absence of FCS.

109 s of objects, the collection and analysis of FCS data have to be reconceptualized.

110 Current analysis of FCS data is largely based on the assumption that the lab

111 Global analysis of FCS data provides association (k(+)) and dissociation (k

112 Here, we extended the applicability of FCS for the detection and analysis of periodically passi

113 foundation for the automated application of FCS to the analysis of biological and other complex samp

114 Finally, task-induced changes of FCS and rCBF in the lateral-parietal lobe positively cor

115 However, the inherent drawback of FCS is that species with similar molecular weight could

116 The finite-domain form of FCS differs from the classical form in its boundary and

117 Interpretation of FCS data relies critically on objective multiple hypothe

118 The unbiased interpretation of FCS data relies on the evaluation of multiple competing

119 complexity to prevent over interpretation of FCS data.

120 toward a model-independent interpretation of FCS experiments: 1) the obtention of correlation data at

121 In order to better understand the limits of FCS, this study systematically explores the relationship

122 In addition, although measurement of FCS remains preferable for assessment of central retinal

123 oscopy (SPIM-FCS), a multiplexed modality of FCS, which generates maps of molecular dynamics, concent

124 In addition to eliminating overfitting of FCS data, the procedure dictates when the interpretation

125 imulations, which predict the performance of FCS under a variety of experimental circumstances.

126 To utilize the power of FCS for systems with nonstochastic displacements of obje

127 ly regulated after growth in the presence of FCS.

128 system was downregulated by the presence of FCS.

129 ue to typically low signal-to-noise ratio of FCS data and correlated noise in autocorrelated data set

130 Moreover, because of the high sensitivity of FCS, this method allows examination of the effect of AMP

131 Our FCS and microfluidic measurements also highlight the key

132 sing rate parameter values determined in our FCS experiments, could reconstruct both the observed mem

133 We find that our FCS estimates are quantitatively consistent with our flu

134 ast to the ~20% enhancement seen in parallel FCS experiments using p-nitrophenyl phosphate (pNPP) as

135 ulated emission depletion FCS for performing FCS at subdiffraction spatial scales.

136 The results show the power of FPA and PET-FCS in solving the trajectory of cotranslational protein

137 The two faster fluctuations observed by PET-FCS, could be attributed to fluctuations within the nati

138 r fluorescence correlation spectroscopy (PET-FCS) to show how a small alpha-helical domain, the N-ter

139 h fluorescence correlation spectroscopy (PET-FCS).

140 o fluorescence correlation spectroscopy (PET-FCS).

141 S and compare PIE-RICS with single-point PIE-FCS measurements.

142 ethod to simulate single- and multiple-point FCS, photon-counting histogram analysis, raster image co

143 Measurement using conventional single-point FCS at every individual pixel results in continuous long

144 the high temporal resolution of single-point FCS while probing different nuclear regions in the same

145 otational diffusion as assessed by polarized FCS and previous multi-frequency (1)H NMR relaxometry ex

146 We present FCS and STED experiments showing that dense membrane bou

147 details of performing point STED-FCS (pSTED-FCS) and scanning STED-FCS (sSTED-FCS) measurements, fro

148 experiment, both FCS-rCBF coupling and rCBF/FCS ratio were modulated by task load in the ECN and/or

149 er rCBF per unit connectivity strength (rCBF/FCS ratio); whereas, this index was lower in posterior v

150 In particular, using real FCS data, we describe how the effects of cell crowding a

151 cal processes, as well as spatially resolved FCS from image correlation spectroscopy, providing an im

152 fusing diffusivity) in variable-length-scale FCS.

153 ast dynamics at the relevant spatial scales, FCS has been combined with super-resolution stimulated e

154 ffected by the presence of fetal calf serum (FCS) in the growth medium.

155 essential medium, MEM) and fetal calf serum (FCS) were probed by XANES and EXAFS.

156 We thus performed simultaneous FCS and FRAP measurements on supported lipid bilayers an

157 n of ice and a freeze-concentrated solution (FCS) are poorly understood in spite of their importance

158 irst used a fully conditional specification (FCS) multiple imputation method to establish complete da

159 oscopy (AFM) based force clamp spectroscopy (FCS) and dynamic force spectroscopy (DFS).

160 rrelation or cross-correlation spectroscopy (FCS or FCCS), a single molecule technique, has the abili

161 ) and fluorescence correlation spectroscopy (FCS) also reveal divalent metal ion (Me(2+))-induced clu

162 mbine fluorescence correlation spectroscopy (FCS) and a microfluidic shear cell to monitor real-time

163 based fluorescence correlation spectroscopy (FCS) and can be isolated on a solid substrate for single

164 used fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleaching (FRA

165 Fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleaching (FRA

166 RAP), fluorescence correlation spectroscopy (FCS) and Forster resonance energy transfer (FRET) enable

167 Using fluorescence correlation spectroscopy (FCS) and membrane-binding kinetic measurements, we ident

168 s are fluorescence correlation spectroscopy (FCS) and photon counting histogram (PCH) analysis.

169 Fluorescence correlation spectroscopy (FCS) and photon counting histogram (PCH) are techniques

170 RAP), fluorescence correlation spectroscopy (FCS) and single-molecule tracking (SMT).

171 ch as fluorescence correlation spectroscopy (FCS) and spectral imaging.

172 Here fluorescence correlation spectroscopy (FCS) and transmission electron microscopy (TEM) are comp

173 used Fluorescence Correlation Spectroscopy (FCS) and two photon excitation to determine the translat

174 ) and fluorescence correlation spectroscopy (FCS) are the two most direct methods to measure the diff

175 e and fluorescence correlation spectroscopy (FCS) assays showed that approximately 20% of the substra

176 from fluorescence correlation spectroscopy (FCS) autocorrelation data.

177 iple, fluorescence correlation spectroscopy (FCS) based methods can provide such data, but live-cell

178 , how fluorescence correlation spectroscopy (FCS) can be used to study binding kinetics of ligands wi

179 that fluorescence correlation spectroscopy (FCS) combined with real-time tracking of the center of m

180 R and fluorescence correlation spectroscopy (FCS) experiments combined with small-angle X-ray scatter

181 with fluorescence correlation spectroscopy (FCS) for simultaneous analysis of labeled and unlabeled

182 years fluorescence correlation spectroscopy (FCS) has become a routine method for determining diffusi

183 Fluorescence correlation spectroscopy (FCS) has permitted the characterization of high concentr

184 ed by fluorescence correlation spectroscopy (FCS) in different alcohol solutions.

185 using fluorescence correlation spectroscopy (FCS) in vitro.

186 Fluorescence correlation spectroscopy (FCS) is a noninvasive technique that probes the diffusio

187 Fluorescence Correlation Spectroscopy (FCS) is a popular tool for measuring molecular mobility

188 Fluorescence correlation spectroscopy (FCS) is a powerful approach to characterizing the bindin

189 Fluorescence correlation spectroscopy (FCS) is a powerful technique to investigate molecular dy

190 Fluorescence correlation spectroscopy (FCS) is a powerful tool to infer the physical process of

191 Fluorescence correlation spectroscopy (FCS) is a sensitive technique commonly applied for study

192 ) and fluorescence correlation spectroscopy (FCS) measurements of the binding kinetics of a transcrip

193 ) and fluorescence correlation spectroscopy (FCS) measurements on single giant unilamellar vesicles (

194 Fluorescence correlation spectroscopy (FCS) methods are powerful tools for unveiling the dynami

195 ques (fluorescence correlation spectroscopy (FCS) or raster-scan image correlation spectroscopy) or p

196 aging fluorescence correlation spectroscopy (FCS) performed using array detectors has been successful

197 e via fluorescence correlation spectroscopy (FCS) provide a sensitive means of probing the underlying

198 nted, fluorescence correlation spectroscopy (FCS) reveals numerous static and dynamic properties of m

199 ition fluorescence correlation spectroscopy (FCS) showed that cytosolic Bax diffuses much slower than

200 hed a fluorescence correlation spectroscopy (FCS) system to measure the percentage of doubly labeled

201 ion of Fluorescent Correlation Spectroscopy (FCS) techniques.

202 used fluorescence correlation spectroscopy (FCS) to accurately and precisely determine the relative

203 Using fluorescence correlation spectroscopy (FCS) to distinguish between different types of diffusion

204 e use fluorescence correlation spectroscopy (FCS) to quantitatively and accurately measure flow speed

205 d for fluorescence correlation spectroscopy (FCS) under pulsed stimulated emission depletion (STED).

206 ; and fluorescence correlation spectroscopy (FCS) was further used to investigate the diffusion of BS

207 Fluorescence correlation spectroscopy (FCS) was used to determine the affinity of parental (hom

208 y and fluorescence correlation spectroscopy (FCS) we found out that, upon contact with the membrane,

209 using fluorescence correlation spectroscopy (FCS) with photon counting histogram (PCH) analysis, a se

210 using fluorescence correlation spectroscopy (FCS) with photon counting histogram (PCH).

211 used fluorescence correlation spectroscopy (FCS), a method that offers single-molecule resolution.

212 ly on fluorescence correlation spectroscopy (FCS), a technique that provides only indirect, ensemble-

213 ed by fluorescence correlation spectroscopy (FCS), a well-established technique based on the analysis

214 ICS), fluorescence correlation spectroscopy (FCS), and atomic force microscopy (AFM).

215 FRET, fluorescence correlation spectroscopy (FCS), and biolayer interferometry to develop a systemati

216 esis, fluorescence correlation spectroscopy (FCS), and microfluidic experiments.

217 Fluorescence correlation spectroscopy (FCS), dynamic light scattering (DLS), and transmission e

218 Using fluorescence correlation spectroscopy (FCS), intermolecular fluorescence resonance energy trans

219 Fluorescence correlation spectroscopy (FCS), is a widely used tool routinely exploited for in v

220 ) and Fluorescence Correlation Spectroscopy (FCS), obtaining estimates in which the FCS value is an o

221 uding Fluorescence Correlation Spectroscopy (FCS), ribosome Run-Off Assays (ROA) after Harringtonine

222 Using fluorescence correlation spectroscopy (FCS), RR1 was shown to detect the presence of as low as

223 , and fluorescence correlation spectroscopy (FCS), to monitor the diverse biophysical states of expan

224 t and fluorescence correlation spectroscopy (FCS), we demonstrated high-affinity labeling of the acti

225 Using Fluorescence Correlation Spectroscopy (FCS), we have characterized the concentration and variab

226 M and fluorescence correlation spectroscopy (FCS), we show that fibril disassembly at pH 6.4 results

227 ed by fluorescence-correlation spectroscopy (FCS), where intensity fluctuations in a small spot are r

228 using fluorescence correlation spectroscopy (FCS), which enables us to monitor the salt-induced compa

229 ed on fluorescence correlation spectroscopy (FCS), which measures antibody-virion binding with all re

230 ) and Fluorescence Correlation Spectroscopy (FCS).

231 olved fluorescence correlation spectroscopy (FCS).

232 ed by fluorescence correlation spectroscopy (FCS).

233 ecule fluorescence correlation spectroscopy (FCS).

234 ed by fluorescence correlation spectroscopy (FCS).

235 ch as fluorescence correlation spectroscopy (FCS).

236 ce by fluorescence correlation spectroscopy (FCS).

237 -fluorescence correlation spectroscopy (SPIM-FCS), a multiplexed modality of FCS, which generates map

238 The FCS diffusion law was applied to SPIM-FCS data to study the subresolution membrane organizatio

239 sSTED-FCS reveals transient molecular interaction hotspots for

240 FCS (pSTED-FCS) and scanning STED-FCS (sSTED-FCS) measurements, from calibration and sample preparati

241 ation spectroscopy (scanning STED-FCS, sSTED-FCS) to characterize the spatial and temporal heterogene

242 STED-FCS has been applied in point or scanning mode to reveal

243 has shown great potential for enhancing STED-FCS, but has also created a demand for software which is

244 correlation of point data derived from STED-FCS experiments.

245 with super-resolution STED microscopy (STED-FCS).

246 e technical details of performing point STED-FCS (pSTED-FCS) and scanning STED-FCS (sSTED-FCS) measur

247 hojnacki et al. employ super-resolution STED-FCS microscopy to study dynamics of Env molecules on HIV

248 point STED-FCS (pSTED-FCS) and scanning STED-FCS (sSTED-FCS) measurements, from calibration and sampl

249 ence correlation spectroscopy (scanning STED-FCS, sSTED-FCS) to characterize the spatial and temporal

250 fluorescence correlation spectroscopy (STED-FCS) to access and compare the diffusion characteristics

251 fluorescence correlation spectroscopy (STED-FCS), a technique which allows the study of membrane dyn

252 ip between functional connectivity strength (FCS) and rCBF during resting and an N-back working-memor

253 In the present study, FCS and PCH were applied to determine the diffusion coef

254 differences for the foveal central subfield (FCS) and total macular volume were computed.

255 ty causes familial chylomicronemia syndrome (FCS), which is associated with very high plasma triglyce

256 s, termed familial chylomicronemia syndrome (FCS); or familial partial lipodystrophy.

257 blished fluorescence fluctuation techniques (FCS, FCCS, and PCH) required fractions between 7 and 11%

258 This study demonstrates that FCS diffusion law analysis is a powerful tool to determi

259 We show by simulations and experiments that FCS can sensitively quantify the flow-rates, variability

260 The discrepancy arises from the fact that FCS and FRAP report on different effective (concentratio

261 Finding that FCS analyses are optimal for both short and long length

262 These data show that FCS analysis of ligand-occupied receptors provides a uni

263 The FCS data in combination with the simulations indicate th

264 The FCS diffusion law was applied to SPIM-FCS data to study

265 The FCS showed a striking spatial correlation with rCBF, and

266 The FCS technique has been significantly advanced by its com

267 ing sites to show that both the FRAP and the FCS estimates may be correct and compatible with the obs

268 s the simplest hypothesis that describes the FCS data based on sampling and signal limitations, natur

269 From the FCS experiment, the tertiary structure of betaLG was obs

270 The results of the FCS analyses and the calculated pristine in-gel diffusio

271 We therefore derived general forms of the FCS and PCH frameworks for bounded systems.

272 PCH analysis of the FCS data were best described by a one-component dimer mo

273 of the nucleus, improper calibration of the FCS focal volume, or the intentional FRAP photobleach.

274 es multiple positions by slowly scanning the FCS observation volume across the nucleus.

275 ker) as a reference, we efficiently sort the FCS segments into different populations and obtain avera

276 to determine membrane heterogeneity via the FCS diffusion laws, showing that there is a lower length

277 copy (FCS), obtaining estimates in which the FCS value is an order of magnitude larger than the FRAP

278 This FCS-based technique establishes a framework for minimall

279 ng a TAT of 60 minutes or less or an on-time FCS was awarded 1 point.

280 as "wheels out" to "wheels in," and on-time FCS, which was defined as "wheels in" within 6 minutes o

281 had higher mean 18-h PR (p < 0.0001) and TIP-FCS (p = 0.002).

282 Future studies of PR and TIP-FCS for elective stenting may facilitate personalized an

283 the relation of platelet aggregation and TIP-FCS to the occurrence of periprocedural infarction.

284 alirudin on platelet reactivity (PR) and TIP-FCS, and their relation to periprocedural infarction in

285 ion; p < 0.001 for all) and reduced mean TIP-FCS (p < 0.05).

286 The PR, TIP-FCS, and myonecrosis markers were serially determined.

287 n-induced platelet-fibrin clot strength (TIP-FCS) measured by thrombelastography in percutaneous coro

288 agonists and the tensile strength of the TIP-FCS, 2 measurements strongly associated with periprocedu

289 Our procedure is generally applicable to FCS and image correlation spectroscopy and therefore pro

290 orter than the time required for traditional FCS analysis at each pixel.

291 se quantification of DNA hybridization using FCS analysis, in which the FBKs play a major role rather

292 Spot variation FCS was in accordance with a model of fast microscopic d

293 P may represent an adequate alternative when FCS is unavailable.

294 While FCS provides estimates of dynamical quantities, such as

295 fusion coefficient maps and is combined with FCS diffusion law analysis to examine subresolution memb

296 ection procedure to overcome this issue with FCS data analysis.

297 Combination of z-STED with FCS and spectral imaging showed diffusion dynamics and l

298 e, we show that a combination of z-STED with FCS or spectral imaging enables us to see previously uno

299 CBD01 and I-BET762 prior to stimulation with FCS and TGF-beta.

300 ime, which are found to match very well with FCS results.