ライフサイエンスコーパス: Fd

1 Fd and Pf4 formed tactoids with different morphologies t

2 Fd, a related bacteriophage of Escherichia coli, has sim

3 Fd-GOGAT is a chloroplastic enzyme responsible for the r

4 Fd-OCT is a promising potential alternative modality to

5 mAb subunits of approximately 25 kDa: Fc/2, Fd', and LC.

6 the paramagnetic oxidized cluster of Pf 3Fe Fd with an intact disulfide bond reported previously are

7 emperature behavior for the forms of the 3Fe Fd with alternate disulfide orientations have been analy

8 ger forms of pFNRII bound more strongly to a Fd affinity column than did the shorter forms, pFNRII(IS

9 n a pFNRII protein which failed to bind to a Fd column.

10 d between oxidized mNT and apo-ferredoxin (a-Fd) using UV-VIS spectroscopy and native-PAGE, as well a

11 ibits transfer of the [2Fe-2S] clusters to a-Fd.

12 ure of the reduced form of the 98-amino acid Fd domain determined by nuclear magnetic resonance (NMR)

13 in the Bcd-EtfA(HQ)B complex and additional Fd(-).

14 giving new evidence of labor division among Fd isoforms.

15 An Fd-OCT instrument with axial resolution of 4 to 4.5 micr

16 ferredoxin (Fd), thioredoxins (Trxs), and an Fd-dependent Trx reductase (FTR), the Fd-FTR-Trxs system

17 n inactive scFab effector arm composed of an Fd region and light chain from two separate binders.

18 Our results demonstrate that an Fd-based intracellular binding protein can find and disa

19 tion 95a, were combined individually with an Fd region known to generate a Hib PS-combining site when

20 fragments (the light chain and the Fc/2 and Fd fragments of the heavy chain) confirms the monoclonal

21 In the presence of both Bcd and Fd, the total beta-FADH(-) of EtfAB bifurcates to afford

22 he N-terminal amino acids of the L chain and Fd remained unchanged, and a random HCDR3 library built

23 The library contained random L chains, and Fd segments enriched in VH domains encoded by the VHX24

24 een the FNR charge-reversal mutant E139K and Fd at 12 mM ionic strength (mu) is extremely impaired re

25 EtfAB bifurcates to afford alpha-FAD(*-) and Fd(-); a second bifurcation yields alpha-FADH(-) in the

26 nts from stratigraphic units G1, Fd/d+G1 and Fd/d, Fd.

27 nt physiological role for the NADH-GOGAT and Fd-GOGAT gene products.

28 NiR), indicating the genes encoding GSII and Fd-NiR in these prasinophytes evolved via vertical trans

29 tible with the idea that C. tepidum Fd I and Fd II accept 2 electrons upon reduction.

30 C. tepidum Fd I and Fd II are novel 2[4Fe-4S] Fds, which were shown previous

31 results suggest that the C. tepidum Fd I and Fd II polypeptides each contain two bound [4Fe-4S] clust

32 The reduction potentials of Fd I and Fd II were determined by cyclic voltammetry to be -514 a

33 Two distinct ferredoxins, Fd I and Fd II, were isolated and purified to homogeneity from ph

34 ny change in binding affinity of the ISU and Fd proteins.

35 act mAb, Fab, or F(ab')2 mass, intact LC and Fd masses, and CDR3 sequence coverage enabled determinat

36 rite reductase, Fd:NADP+ oxidoreductase, and Fd:thioredoxin reductase.

37 ctron transfer pathways to the NAD(P)(+) and Fd reduction sites by a mechanism that is fundamentally

38 ier for two oxidoreductases from Pf, POR and Fd:NADP oxidoreductase, and the POR holoenzyme displayed

39 tron flow between mutant pFNRII proteins and Fd, showed there was a variation in k(obs) from 76 to 26

40 Thus, the NTRC, 2-Cys Prxs, and Fd-FTR-Trxs redox systems may act concertedly, but the n

41 oxidation-derived redox pair, DsrC(red) and Fd(red), relative to the Qmo-MQ-Qrc (quinone-interacting

42 ities, i.e., H(2)-dependent Fd reduction and Fd(2-)-dependent H(2) production.

43 these domains induced potent anti-IZ or anti-Fd antibody responses in animals when fused to an HIV-1

44 AP7 Fd (VH domain + Cgamma1 domain) segments containing thes

45 does not promote cluster transfer to an apo Fd target.

46 wild-type Hs ISU cluster transfer to Hs apo Fd, k(2) approximately 540 M(-1) min(-1), relative 56 M(

47 o be independent of the concentration of apo Fd over the range of 1-25 microM.

48 change in the affinity of binding to the apo Fd since k(2) was found to be independent of the concent

49 ermined for cluster transfer from ISU to apo Fd for both Homo sapiens and Schizosaccharomyces pombe p

50 Fe-S cluster transfer from holo IscU to apo Fd in the presence of chaperone DnaK demonstrate an inhi

51 Cluster transfer from ISU1 to apo Fd is demonstrated: the first example of transfer from a

52 s rate constants for cluster transfer to apo Fd targets from Homo sapiens and S. pombe demonstrated t

53 rate of iron-sulfur cluster transfer to apo Fd.

54 sites for binding of holo ISA and ISU to apo Fd.

55 er from Schizosaccharomyces pombe ISA to apo Fd.

56 noglobulin heavy chain gene and displayed as Fd coat protein fusion constructs of M13 phage.

57 ansporter 1, and CTBP2 label foveal cones at Fd 88 and cones at the retinal edge around birth.

58 A foveal pit begins to form at Fd 135 in the center of the foveal avascular zone which

59 es and rod opsin in rods around the fovea at Fd 100.

60 odified version of filamentous bacteriophage Fd that produces significantly higher colorimetric signa

61 accounts for most of the difference between Fd and HiPIP reduction potentials.

62 affected and where complex formation between Fd and the enzyme prevented the inhibition by either rea

63 nteraction and the electron transfer between Fd and FNOR.

64 At birth (Fd 144) the fovea has a single layer of cones over the p

65 cein angiography was detected in all eyes by Fd-OCT.

66 tion of a lysine, K436, that is protected by Fd against labeling.

67 inines, R375 and R556, that are protected by Fd against labeling.

68 es with recombinant baculoviruses containing Fd and AP7 kappa chain cDNA.

69 om stratigraphic units G1, Fd/d+G1 and Fd/d, Fd.

70 ferredoxin from Pyrococcus furiosus (Pf D14C Fd).

71 The incipient fovea is present at fetal day (Fd) 100 as the only part of the retina that contains fiv

72 s and wholemounts of retinas from fetal day (Fd) 65 to adult animals were analyzed immunohistochemica

73 cones across 70% of the retina at fetal day (Fd) 88, indicating that it is expressed shortly after ph

74 nas from Macaca monkeys from ages fetal day (Fd) 89 through adulthood were double labeled using immun

75 s generated first in the fovea at fetal day (Fd)70-74 and with the last generated at the retinal edge

76 e resulted in a pFNRII enzyme with decreased Fd affinity.

77 the fluorescence difference signal DeltaFd (Fd = F500 - F400).

78 ysiological activities, i.e., H(2)-dependent Fd reduction and Fd(2-)-dependent H(2) production.

79 The cubic diamond (Fd 3 m) group IVA element Si has been the material drive

80 Frequency-domain (Fd)OCT line scans were obtained from seven patients with

81 contrasts with GLU1, the major gene encoding Fd-GOGAT, which is most highly expressed in leaves and i

82 exergonic NAD(P)(+) reduction and endergonic Fd reduction modes.

83 Ferredoxin (Fd) and ferredoxin:NADP(+) reductase (FNR) from Anabaena

84 Ferredoxin (Fd) is the major iron-containing protein in photosynthet

85 the ability of reduced [2Fe-2S] ferredoxin (Fd) to act as a catalytically competent electron donor d

86 with reduced plant-type [2Fe-2S] ferredoxin (Fd).

87 D-containing flavin domain and a ferredoxin (Fd) domain carrying a [2Fe-2S] cofactor.

88 iron-sulfur protein (HiPIP) and ferredoxin (Fd) active sites.

89 and a cellular reductant such as ferredoxin (Fd) as co-substrates.

90 ted RapJ and RapN, an associated ferredoxin (Fd) RapO, and three potential SAM-dependent O-methyltran

91 of the [2Fe-2S] vegetative cell ferredoxin (Fd) from Anabaena PCC 7120, each of which has a cluster

92 hree-iron, single cubane cluster ferredoxin (Fd) from the hyperthermophilic archaeon Pyrococcus furio

93 The single cubane cluster ferredoxin (Fd) from the hyperthermophilic archaeon Pyrococcus furio

94 studied [Fe4S4] model complexes, ferredoxin (Fd), and to new data on high-potential iron-sulfur prote

95 he physiological electron donor, ferredoxin (Fd), most favorably interacts with this cluster.

96 d by phages whose genomes encode ferredoxin (Fd) electron carriers.

97 Pyrococcus furiosus ferredoxin (Fd) contains a single [Fe(4)S(4)] cluster coordinated by

98 l electron acceptor, P. furiosus ferredoxin (Fd), has been established from an FOR-Fd cocrystal struc

99 e of the paramagnetic, four-iron ferredoxin (Fd) from the hyperthermophilic archaeon Thermococcus lit

100 Spinach (Spinacea oleracea) leaf ferredoxin (Fd)-dependent nitrite reductase was treated with either

101 educed by the plastid-localized, ferredoxin (Fd)-dependent enzyme P Phi B synthase to yield 3Z-P Phi

102 CO2, coupled to the reduction of ferredoxin (Fd).

103 radient regulation-like1 (PGRL1) ferredoxin (Fd) pathway, involved in recycling excess reductant to i

104 tion which reduces low potential ferredoxin (Fd) and high potential crotonyl-CoA using NADH as an ele

105 kely functions as proton-pumping ferredoxin (Fd): type-I cytochrome c oxidoreductase, which transitio

106 e-bound enzymes that use reduced ferredoxin (Fd) as an electron donor to reduce protons to molecular

107 otein (e.g., CODH/ACS or reduced ferredoxin (Fd)) to the FeS cluster which then directs electrons to

108 es on photosynthetically reduced ferredoxin (Fd), thioredoxins (Trxs), and an Fd-dependent Trx reduct

109 l truncation on interaction with ferredoxin (Fd), recombinant pFNRII proteins, differing by deletions

110 Ferredoxin, Fd, is often deficient in metronidazole-resistant strain

111 dABC that reduces low-potential ferredoxins (Fd) by oxidizing hydrogen gas (H(2)).

112 s from NADPH to hydrogenase via ferredoxins (Fd).

113 Two distinct ferredoxins, Fd I and Fd II, were isolated and purified to homogeneit

114 Z) and the T4 bacteriophage fibritin foldon (Fd) trimerization domains.

115 ontrast, uncleaved, histidine-tagged Foldon (Fd) domain-containing gp140 proteins (gp140UNC-Fd-His),

116 Arabidopsis contains two expressed genes for Fd-GOGAT (GLU1 and GLU2).

117 a mutation in only one of the two genes for Fd-GOGAT leads to a photorespiratory phenotype in the gl

118 tion K56A/K60A decreased the k(cat)/K(M) for Fd by 250-fold, whereas the triple mutation K56A/K60A/K2

119 30A on Delta9D decreased the k(cat)/K(M) for Fd by 4-, 22-, and 2400-fold, respectively, as compared

120 56A/K60A/K230A decreased the k(cat)/K(M) for Fd by at least 700 000-fold.

121 light of the presently available models for Fd-mediated electron transfer in Delta9D and other prote

122 ite to the significant increase observed for Fd, where the [Fe4S4] cluster is solvent exposed.

123 ent Km = 1.0 microM at 80 degreesC) than for Fd (apparent Km = 4.4 microM).

124 doxin (Fd), has been established from an FOR-Fd cocrystal structure.

125 mide revealed that carboxylate residues from Fd and lysine residues from Delta9D contribute to cross-

126 bilizing interactions in Pyrococcus furiosus Fd, however, lead to strong destabilization of the disul

127 s distinguished AlkB that couples with fused Fd-Fdr reductases from AlkB with alternate architectures

128 hic bone points from stratigraphic units G1, Fd/d+G1 and Fd/d, Fd.

129 abine, busulfan, and antithymocyte globulin (Fd/Bu/ATG) was associated with the lowest TRM of 11.6% (

130 e used to complement a conditional T. gondii Fd KO strain.

131 are abundant in uncleaved CZA97.012 gp140UNC-Fd-His proteins but very rare in native-like trimer popu

132 ) domain-containing gp140 proteins (gp140UNC-Fd-His), based on the same env genes, very rarely form n

133 ume of a single-diamond network (space group Fd 3 m) templated into gold from a triblock terpolymer c

134 in ordinary cubic (c-) diamond (space group: Fd-3m).

135 (X), supports the F(X) --> F(A) --> F(B) --> Fd sequence of electron transfer on the acceptor side of

136 Cross-linking of holo ISU1 with holo Fd is consistent with a role for redox chemistry in clus

137 k(2) approximately 169 M(-1) min(-1) for Hs Fd).

138 covalency is much lower in natively hydrated Fd active sites than in HiPIPs but increases upon water

139 e S K-edge XAS of the protein ferredoxin II (Fd II) from the D. gigas active site shows a decrease in

140 the characterization of mutants deficient in Fd-GOGAT enzyme activity (gls).

141 roduced to detect variants and impurities in Fd, Fc/2, and LC subunits of an IgG1 consistently across

142 f the activity of hydrogenosomal proteins in Fd KO cells show a fourfold increase in hydrogenase acti

143 aK reduces the rate of formation of the IscU-Fd complex (greater than 8-fold), but has little influen

144 analysis of these ferredoxins by localizing Fd, Fdx2, Fdx3, and Fdx6 to the chloroplast by using iso

145 ain-switched Fab fragment containing the M77 Fd fragment and a different light chain was able to reco

146 urements; and (2) that an alternative, minor Fd isoform has been overexpressed in plants, giving new

147 In the mitochondria, Fd-GOGAT interacts physically with SHMT1, and this inter

148 one found here for M. capsulatus (Bath) MMOR-Fd.

149 protein into its functional ferredoxin (MMOR-Fd) and FAD/NADH (MMOR-FAD) component domains.

150 The 10.9 kDa MMOR-Fd (MMOR residues 1-98) and 27.6 kDa MMOR-FAD (MMOR resi

151 f MMOH and the [2Fe-2S] domain of MMOR (MMOR-Fd).

152 ther to explore the modularity of MMOR, MMOR-Fd and MMOR-FAD were connected in a non-native fashion.

153 close to one another on the surface of MMOR-Fd and >25 A from the [2Fe-2S] cluster.

154 forms cross-links to the side chains of MMOR-Fd residues Glu-56 and Glu-91.

155 transfer from MMOR-FAD(hq) to oxidized MMOR-Fd is extremely slow (k = 1500 M(-1) s(-1) at 25 degrees

156 rved in those cells expressing a non-mutated Fd (UMHC) or in IPTG-treated non-transduced V79 cells.

157 irectly involved in electrostatic binding of Fd to the enzyme.

158 ps on Fd may situate the [2Fe-2S] cluster of Fd closer to W62, a surface residue that is structurally

159 A library of Fd fragment antibody binding proteins was created by ran

160 and immunoblot analyses confirm the loss of Fd mRNA and protein in pKO-FD-NEO cells.

161 so measured the midpoint redox potentials of Fd and Fdx2 and determined the kinetic parameters of the

162 The reduction potentials of Fd I and Fd II were determined by cyclic voltammetry to

163 rogen production kinetics in the presence of Fd, ferredoxin:NADP(+)-oxidoreductase (FNR), and NADP(+)

164 m of oxidized Fd I closely resembled that of Fd II; however, both spectra appeared to be unique relat

165 A K(m) for spinach (Spinacea oleracea) Fd was determined to be 3 to 4 microM.

166 ated with growth pattern and size of CNVM on Fd-OCT, with correlation being stronger for classic lesi

167 en K56 and K60 and the carboxylate groups on Fd may situate the [2Fe-2S] cluster of Fd closer to W62,

168 giographic CNVM size correlated with size on Fd-OCT but correlation was better for classic CNVM (clas

169 echanism that does not require either PFO or Fd for metronidazole activation.

170 s indicated that the CD spectrum of oxidized Fd I closely resembled that of Fd II; however, both spec

171 ng illumination confirmed that the minor pea Fd isoform promotes enhanced cyclic flow around photosys

172 All Pf Fd forms with an intact disulfide bond exhibit a dynamic

173 are found in other hyperthermostable Fds, Pf Fd has two elements, the long helix and the triple-stran

174 n a longer helix and larger beta-sheet in Pf Fd, relative to other hyperthermostable Fds, more likely

175 clusters in wild-type and mutant forms of Pf Fd with Asp, Ser, Cys, Val, His, Asn, and Tyr residues o

176 RecPOR-delta replaced Pf Fd as an in vitro electron carrier for two oxidoreductas

177 may be relevant to electron transfer with Pf Fd partners.

178 Here, we show that PGR5/PGRL1-Fd CEF functions in accordance with an ATP/redox control

179 ogether, our results suggest that PGR5/PGRL1-Fd CEF is most required under conditions when Fd becomes

180 natural carbohydrate decorated form of PSM (Fd-PSM) containing 40% of the core 1 blood group type A

181 Additionally, pssm2-Fd exhibited a low midpoint reduction potential (-336 mV

182 ain deficient in sulfite assimilation, pssm2-Fd complemented bacterial growth when coexpressed with a

183 ctural analysis of myovirus P-SSM2 Fd (pssm2-Fd), which infects the cyanobacterium Prochlorococcus ma

184 inus sulfite reductase, revealing that pssm2-Fd can transfer electrons to a host protein involved in

185 ctase, which transitions to a proton-pumping Fd(red): nicotinamide adenine dinucleotide (NAD(+)) oxid

186 at the structural alternations in Pyrococcus Fd relative to other hyperthermostable Fds are not to in

187 Hdr-Flox-1 is postulated to recycle Fd(red) via a flavin-based electron bifurcation involvin

188 P(+) oxidoreductase could use NADH to reduce Fd and thus facilitate ADO-mediated alkane production.

189 Reduced Fd does not readily reduce ISU-bound [2Fe-2S](2+) and do

190 order of addition for substrate and reduced Fd showed high conversion of 18:0-ACP to 18:1-ACP (appro

191 enzyme-substrate complex with excess reduced Fd, a slower, linear rate (k(obsd) = 3.4 s(-1)) of produ

192 t rates of direct electron loss from reduced Fd to O2 However, this loss does not interfere with meas

193 say uses dithionite (DTH) to provide reduced Fd.

194 a a collisional interaction with the reduced Fd present in the unreactive complex.

195 er) when 18:0-ACP was added prior to reduced Fd.

196 idized enzyme-substrate complex with reduced Fd were studied by rapid-mix and chemical quench methods

197 ing a fused ferredoxin-ferredoxin reductase (Fd-Fdr).

198 for ferredoxin-dependent nitrite reductase (Fd-NiR), indicating the genes encoding GSII and Fd-NiR i

199 eracting proteins, namely nitrite reductase, Fd:NADP+ oxidoreductase, and Fd:thioredoxin reductase.

200 lier proposal that the structure of the same Fd with the R disulfide orientation resembles that of th

201 we overexpressed a minor pea (Pisum sativum) Fd isoform (PsFd1) in tobacco (Nicotiana tabacum) plants

202 Despite genetic evidence for a single Fd-GOGAT locus and gene, we discovered that Arabidopsis

203 k(2) approximately 170 M(-1) min(-1) for Sp Fd and k(2) approximately 169 M(-1) min(-1) for Hs Fd).

204 Structural analysis of myovirus P-SSM2 Fd (pssm2-Fd), which infects the cyanobacterium Prochlor

205 other RIC regimen demonstrated superiority, Fd/Bu/ATG should be considered as baseline in future pro

206 Surprisingly, Fd KO cells are not resistant to metronidazole under aer

207 Ferredoxin-dependent glutamate synthase (Fd-GOGAT) plays a major role in photorespiration in Arab

208 des Ferredoxin-dependent Glutamate Synthase (Fd-GOGAT).

209 here the rates differed for Hs and Sp target Fd's, suggesting distinct binding sites for binding of h

210 le, compatible with the idea that C. tepidum Fd I and Fd II accept 2 electrons upon reduction.

211 C. tepidum Fd I and Fd II are novel 2[4Fe-4S] Fds, which were shown

212 These results suggest that the C. tepidum Fd I and Fd II polypeptides each contain two bound [4Fe-

213 approximately 2.3-fold higher affinity than Fd II.

214 wo novel features of this work are: (1) that Fd levels achieved in transplastomic plants promote an a

215 Kinetic measurements indicated that Fd I had approximately 2.3-fold higher affinity than Fd

216 We show that Fd-GOGAT is dual targeted to the mitochondria and the ch

217 The Fd and FAD domains have absorbance spectral features ide

218 The Fd family is composed of several isoforms that share hig

219 The Fd/light chain interface of the scFab harbors repulsive

220 The Fd[Formula: see text]m phase at 500 GPa forms buckled ho

221 region of the pFNRII protein in binding the Fd partner.

222 this construction, genomic DNA encoding the Fd fragment of humanized anti-Fc gammaRI mAb, H22, which

223 vents the backflow of the electrons from the Fd reduction branch toward the FMN site, providing a bas

224 and an Fd-dependent Trx reductase (FTR), the Fd-FTR-Trxs system, which links redox regulation to ligh

225 elength-selective (S) opsin are found in the Fd 105 fovea.

226 ts were shown to contain the NEO gene in the Fd locus and to have completely lost the Fd gene.

227 he protein-protein interface involved in the Fd-Delta9D complex by the use of chemical cross-linking,

228 the Fd locus and to have completely lost the Fd gene.

229 imately 2.6 and approximately 2.0 kBp of the Fd 5' and 3' flanking regions (pKO-FD-NEO) was introduce

230 The binding of the Fd domain with the hydroxylase was investigated by NMR s

231 pombe demonstrated that the identity of the Fd is less critical for promoting cluster transfer from

232 disulfide orientation resembles that of the Fd upon cleaving the disulfide bond.

233 ess stable R disulfide resembles that of the Fd with a cleaved disulfide bond.

234 are present at these positions in all of the Fd-dependent nitrite reductase for which sequences are a

235 The critical residues on the Fd domain involved in this binding interaction map well

236 a specific intracellular target and once the Fd expression is repressed the activity of intracellular

237 5'-phosphate under conditions where only the Fd-binding affinity of the enzyme was affected and where

238 the parental strain, demonstrating that the Fd gene product eliminated in KO cells is neither necess

239 It is proposed that the Fd-FTR-Trx and NTRC redox systems are linked by the redo

240 mutations affected binding of pFNRII to the Fd column.

241 ture complex biantennary N-glycan within the Fd segment.

242 nd that the decreased et reactivity of these Fd mutants toward FNR may be due to small changes in the

243 Several minor structural adjustments in Tl Fd relative to other Fds, in addition to the third stran

244 he termini incorporates a third strand in Tl Fd.

245 Comparison of the structure of Tl Fd to those of mesophilic ferredoxins reveals that Tl Fd

246 s to the extraordinary thermostability of Tl Fd.

247 se of mesophilic ferredoxins reveals that Tl Fd possesses the same secondary structural elements, two

248 To analyze the sites which contribute to Fd binding at the pFNRII N-terminal, three mutants were

249 nities of SBA and VML for Tn-PSM relative to Fd-PSM indicate the importance of carbohydrate compositi

250 ctron donor to nitrite reductase relative to Fd.

251 Retinal length increases sharply up to Fd 135, but undergoes a quiescent period around birth du

252 equency domain optical coherence tomography (Fd-OCT) scans (Spectralis HRA+OCT; Heidelberg Engineerin

253 nit suggest that it evolved from an 8Fe-type Fd by the addition of approximately 40 residues at the N

254 ipapillary RNFL thickness was measured using Fd-OCT in 16 segments from 4 quadrants--temporal (316 de

255 d CEF is most required under conditions when Fd becomes overreduced and photosystem I is subjected to

256 To directly test whether Fd is essential for metronidazole susceptibility, gene r

257 and V(m) values in a coupled assay in which Fd was reduced by pyruvate ferredoxin oxidoreductase (PO

258 in the formation of a catalytic complex with Fd.

259 ficantly impaired in its et interaction with Fd at low FNR concentrations and low mu values, the depe

260 , 2.7) and a trend to improved outcomes with Fd/Bu/ATG (RR, 0.58).

261 ickness of RNFL measurements on testing with Fd-OCT.

262 preciably less stable in solution than is wt Fd.

263 is not significantly altered from that of wt Fd (-384 mV), the potential of the C49S mutant (-329 mV)

264 the variants, the reduction potential of WT Fd makes it the most appropriate protein to both accept

265 mutant is virtually identical to that of wt Fd.

266 ct disulfide bond and to the 4Fe oxidized WT Fd (1H and 15N) with a cleaved disulfide bond.

267 ffer from each other and from wild-type (wt) Fd.