ライフサイエンスコーパス: G418

1 G418 also interfered with the infection of cells express

2 G418 binds to both the human and E. coli rRNA A sites wi

3 G418 failed to activate PI-PLC when ConA was present.

4 G418 increased read-through in selenium-replete cells as

5 G418 selected cardiomyocytes were tested for their abili

6 G418 treatment increased immunodetectable endogenous or

7 G418 was more effective than gentamicin (~50% rescue ver

8 G418, which appears to act on the enzyme.substrate compl

9 G418-resistant A9 cell transformants expressing Ad2 rece

10 G418-resistant cells selected following transfection wit

11 G418-resistant colonies were selected and further select

12 G418-resistant ES cell clones are induced to differentia

13 ning the TRES, gave titers of 10(4) to 10(5) G418-resistant CFU/ml on murine NIH 3T3 cells.

14 1q25 deleted region, were retrofitted with a G418 resistance marker and transfected into murine A9 fi

15 2, and several clones were established after G418 selection.

16 94X mice with the nonsense suppression agent G418 resulted in full-length MeCP2 protein production, d

17 Although G418 exhibits the strongest activity, it is very cytotox

18 readthrough agents, including aminoglycoside G418 and suppressor tRNA, enabling a path toward target-

19 unexpected discovery that the aminoglycoside G418 (Geneticin) interferes with the ability of stably t

20 n combination with either the aminoglycoside G418 or an anticodon edited aa-tRNA, which stimulate rea

21 AR1 are hypersensitive to the aminoglycoside G418.

22 Se metabolism, we tested the aminoglycosides G418 and gentamicin in hepatoma cell lines (HepG2, Hep3B

23 We found that the aminoglycosides G418 and gentamicin rescued the expression of the progra

24 C-SIN amphotropic G418-resistant virus particles were generated with a tit

25 nsion and selection with the neomycin analog G418, cells derived from transduced progenitor cells wer

26 ants were selected using the neomycin analog G418.

27 neo gene are selected using the neo analogue G418.

28 ation to resistance to the neomycin analogue G418 were measured, and the nature of the mutations was

29 d-ornithine (d-Orn), a substrate analog, and G418 (Geneticin), a weak non-competitive inhibitor, was

30 more-detailed investigation of ataluren and G418 effects on read-through.

31 After transfection, blasticidin and G418-resistant parasites tested positive for plasmid rep

32 dicines Agency for the treatment of DMD, and G418, a structurally dissimilar aminoglycoside.

33 MCF viruses encoding beta-galactosidase and G418 resistance.

34 ificantly lower than those of gentamicin and G418.

35 ide antibiotics kanamycin A, gentamycin, and G418 stimulated PI-PLC cleavage of the GPI anchor of var

36 ropriate selection (vincristine for MDR1 and G418 for neor).

37 d cell sorting, fluorescence microscopy, and G418 selection.

38 of the 2-DOS aminoglycosides paromomycin and G418 (geneticin) are compared, using both human and Esch

39 Paromomycin and G418 differ with respect to their specificities of actio

40 esis inhibitors hygromycin B, puromycin, and G418.

41 duce TXA(2) was created by gene transfer and G418 selection.

42 903, consistently suppressed G418 uptake and G418-induced PTC readthrough in the DMS-114 cancer cell

43 The aminoglycoside antibiotic G418 is a highly specific and potent inhibitor of STIM2-

44 transferase, for selection by the antibiotic G418, are expressed as a fusion, beta Geo.

45 are put under selection with the antibiotic G418, cell lines can be obtained at high frequency that

46 lected for in the presence of the antibiotic G418.

47 istance in mammalian cells to the antibiotic G418.

48 s were obtained by screening with antibiotic G418.

49 achieved by electroporation and antibiotic (G418) selection.

50 able markers and selected in the antibiotics G418 or blasticidin, respectively, displayed the lowest

51 As G418 treatment had no discernible effect on cellular PrP

52 Aminoglycosides such as G418 permit PTC readthrough and so may be used to addres

53 PI-PLC was activated by G418 even in the presence of the inhibitor VP-616L.

54 omogeneous FGSC cultures were established by G418 selection and continued to express ziwi for more th

55 signal sequence into HepG2 cells followed by G418 selection.

56 Resistant replicon RNAs were generated by G418 selection in the presence of SCH6 in a dose-depende

57 ed activity of protein variants generated by G418-mediated suppression.

58 onally, the extent of readthrough induced by G418 and an NMD inhibitor (SMG1i), alone and in combinat

59 ides a potential mechanism for inhibition by G418 and suggests that allosteric inhibition from this s

60 silencing of STIM2 by siRNA or inhibition by G418 suppresses store-operated Ca(2+) entry and agonist-

61 Clones were obtained by G418 selection and screened for IRF-1 mRNA expression by

62 ch integration had occurred were selected by G418 resistance.

63 hEST2 and individual clones were selected by G418.

64 ailability, and was completely suppressed by G418 under Se-poor conditions.

65 ty of the HK2 Bogota reporter was tracked by G418 resistance.

66 two synthetic CB1 and CB2 peptides, CB1I397-G418 and CB2I298-K319, respectively, in membrane mimetic

67 When combined, G418 and SMG1i increase readthrough product levels in an

68 40 nt, alpha2A/D-AR) and a vector conferring G418 resistance.

69 a3 gene, and a Pgk-neo cassette that confers G418 resistance to mammalian cells.

70 he neo(R) expression cassette, which confers G418 resistance, was used to select for illegitimate int

71 After culture in media containing G418 for 3 weeks, approximately 0.1% of cells previously

72 study, we generated quasiviruses containing G418-selectable HPV-31 genomes with phosphodeficient phe

73 In contrast, G418 increases functional near-cognate tRNA mispairing w

74 of colonies that were resistant to the drug G418, a neomycin analog.

75 treated with the readthrough-promoting drug, G418, accurately predicted readthrough of premature term

76 ed resistance to concentrations of the drugs G418, 6-thioguanine, puromycin and hygromycin well above

77 wo translational readthrough inducing drugs (G418 & PTC124) to investigate potential restoration of e

78 e upstream and downstream CFTR codons during G418-mediated suppression.

79 ved by culturing heterozygotes with elevated G418.

80 motifs were replaced by a cassette encoding G418 resistance and beta-galactosidase, under the contro

81 in unselected cell populations and following G418 selection to obtain uniformly transduced cell popul

82 ent from those recently identified following G418-mediated suppression of the CFTR G542X UGA mutation

83 Electron density for G418 was observed at the boundary between the two domain

84 eading frame were recovered by selecting for G418-resistant colonies.

85 quasivirus infection model and selection for G418 resistant genomes, we demonstrated that Y138 is a c

86 ysis of the HCV replicon RNAs recovered from G418-resistant Huh7 cells revealed several interesting f

87 exes with several aminoglycosides (geneticin G418, paromomycin, and hygromycin B) and the antimalaria

88 Geneticin (G418) was then identified as a potent inducer of the Del

89 ide antibiotic read-through agent geneticin (G418) across a diverse range of in vitro reporter assays

90 now available that gentamicin and Geneticin (G418) can induce the mammalian ribosome to suppress dise

91 doxycycline, chloramphenicol, and Geneticin (G418) interfered with insertion of selenocysteine (Sec),

92 itors in culture, blasticidin and geneticin (G418), were selected for further study.

93 fied minimal media that contained geneticin (G418).

94 some in complex with paromomycin, geneticin (G418), gentamicin, and TC007, solved at 3.3- to 3.7-A re

95 the Leishmania ribosomal A site: Geneticin (G418), a potent aminoglycoside for the treatment of leis

96 to zeocin, puromycin, hygromycin, geneticin/G418, and blasticidin, respectively.

97 pterin, and 1.6 x 10(-5) M thymidine (HAT) + G418; and (c) the presence in genomic DNA of genes for b

98 In G418-resistant lines of N2a or CAD5 cells, the presence

99 Transduced cells were cloned in G418 and expanded for analysis of IGF-1 transgene expres

100 Culture in G418 resulted in elimination of the mesenchymal cells, l

101 red, allowing for selection of revertants in G418.

102 The feeder cell line was selected in G418 and engineered to express zebrafish leukemia inhibi

103 HBV genome and the neo gene and selected in G418 to generate stable cell lines.

104 The transduced cells were selected in G418, an antibiotic toxic to normal mammalian cells.

105 ading frame of the neo gene were selected in G418, and mutation rates were determined by fluctuation

106 r, and stable transformants were selected in G418.

107 ficantly impaired detection (by selection in G418) of genomic events associated with reactivation of

108 ontains a neo gene for positive selection in G418, an I-SceI endonuclease recognition sequence for in

109 After selection in G418, more than 90% of the cells contained the TK gene a

110 ls could not be propagated upon selection in G418-containing media, suggesting nuclear accumulation o

111 Upon selection in the presence of increasing G418 concentrations, transduced NK cells were able to pr

112 Interestingly, G418 had minimal to no effect on protease-resistant PrP

113 se resistance to nourseothricin or kanamycin/G418 to select for propagation in prototrophic hosts.

114 o demonstrate that both aminoglycosides like G418 and more hydrophobic molecules like ataluren stimul

115 ne was selected in vitro using 800 microg/ml G418.

116 body recognition are E2 residues L413, N415, G418, and W420.

117 puromycin-resistant 293 cells) and MDBK-Neo (G418-resistant MDBK cells) cell lines for total cellular

118 transfectants and a population of neomycin (G418)-resistant cells were used as donors for nuclear tr

119 ontaining the enhanced GFP and the neomycin (G418) resistance gene.

120 No G418-resistant colonies were present in cultures infecte

121 No G418-resistant progenitor cell colonies were present in

122 neomycin, an aminoglycoside antibiotic (not G418-neomicin), resulted in reduced KSHV latent gene exp

123 The ability of G418, gentamicin, and PTC124 to induce readthrough was s

124 ned in either the presence or the absence of G418 sulfate and were passaged weekly.

125 after 6 months in culture in the absence of G418 sulfate, approximately 90% of the cells in each clo

126 Intraperitoneal administration of G418 in Mecp2R294X mice was sufficient to elicit full-le

127 were confirmed by Southern blot analysis of G418- and 6-thioguanine (6TG)-resistant clones.

128 By contrast, the binding of G418 induces the destacking of base 1492 when either rRN

129 that are amplified in high concentrations of G418.

130 Se content of G418-induced SELENOP was dependent on Se availability, a

131 We studied the effects of G418, an aminoglycoside, on cellular glutathione peroxid

132 nally, intracranial ventricular injection of G418 in Mecp2R294X mice induced expression of full-lengt

133 50 micromol/L, induced > or = 90% killing of G418-selected cells without affecting nontransduced cell

134 pe HCV-N drastically decreased the number of G418-resistant colonies obtained following transfection

135 AGS1/RASD1 markedly diminished the number of G418-resistant colonies, whereas the Ras subgroup member

136 colony assay did not increase the number of G418-resistant colonies.

137 crease by orders of magnitude the numbers of G418-resistant colonies selected following transfection

138 Significantly higher numbers of G418-resistant colonies were produced in cultures initia

139 Our analysis detected a population of G418(S)/neo(+) Tf1 integration events that would have be

140 lines of N2a or CAD5 cells, the presence of G418 reduced levels of protease-resistant PrP following

141 sciatic nerve were grown in the presence of G418, and within 8-9 d exposure to antibiotic, 99% of al

142 bencephalon were cultured in the presence of G418, surviving cells differentiated into OLs, indicatin

143 Selection was in the presence of G418.

144 verall, neither the frequency of recovery of G418-resistant colonies nor the sizes of NHEJ-associated

145 iterative transfections and the selection of G418-resistant cell clones, form two groups associating

146 ) selectable marker resulted in selection of G418-resistant cell lines that effectively colonize reci

147 lar in vitro and ex vivo activity to that of G418, while its cell toxicity is significantly lower tha

148 Of 11 clones selected by virtue of G418 resistance and positive EpoR expression, 5 clones s

149 ility to confer resistance to blasticidin or G418, respectively.

150 presence of doxycycline, chloramphenicol, or G418, the Sec-containing form of TR1 decreased, whereas

151 '-carbon of gentamicin X2 (GenX2) to produce G418 during the biosynthesis of gentamicins.

152 pendent Sec incorporation is able to repress G418-induced UGA readthrough as well as eRF1-induced sti

153 The resulting G418-sensitive homozygous mutants should allow mutagenes

154 Since G418 stimulates a purified enzyme that is not involved i

155 suppressor (Glu) are expressed from a single G418 selectable vector, while a gene engineered to conta

156 However, the standard G418-resistance cassette used to produce knockout mutant

157 ainst gentamicin and its related structures, G418 and sisomicin, are also associated with islet autoi

158 5 inhibitor, AC1903, consistently suppressed G418 uptake and G418-induced PTC readthrough in the DMS-

159 th established prion infection, arguing that G418 selectively interferes with de novo prion infection

160 ment of patient cell lines demonstrated that G418 increases SMN levels and is a potential lead compou

161 Finally, we find that G418-induced miscoding alters gene expression with subst

162 These observations indicate that G418 is an allosteric activator of Bacillus cereus PI-PL

163 mass spectrometry experiments indicated that G418 caused a substitution of l-arginine for selenocyste

164 These data show that G418 can affect the biosynthesis of this key antioxidant

165 vels or its localization, this suggests that G418 may specifically target prion assemblies or process

166 The G418-resistant (G418R)-LNCaP clones that expressed a hig

167 Further RNA analysis from the G418(S)/neo(+) clones revealed 50% of clones expressing

168 in the expression of receptor protein in the G418-resistant transfectants (nt 74, 100%; nt 143, 80%;

169 was highly effective (99.4 to 99.9%) in the G418-selected cultures.

170 hich induced resistance of infected cells to G418.

171 licons were selected by constant exposure to G418 sulfate.

172 genes neo and ble, conferring resistance to G418 and phleomycin, respectively, 1 kb downstream of "s

173 tein (GFP) and neo (conferring resistance to G418).

174 y, transformation by v-src and resistance to G418, following infection of cells.

175 integrants can be selected by resistance to G418.

176 Cellular clones resistant to G418 antibiotic were selected and expanded for further a

177 NOP levels increased strongly in response to G418, whereas expression of the glutathione peroxidases

178 also capable of replication and gave rise to G418-resistant cell clones following transfection of Huh

179 ere differentiated in vitro and subjected to G418 selection.

180 lational read-through inducing drugs (TRIDs) G418 and PTC124 (Ataluren), we were able to restore up t

181 quence, cells were subsequently placed under G418 selection and conversion confirmed at the genetic l

182 Using G418 selection, we routinely obtained vector particles p

183 ently subjected to positive selection (using G418 to select for neomycin-resistance) and negative sel

184 leukin-6 (IL-6), and IL-3, the cultures were G418-selected, and then challenged with HIV-1(JR-FL) and

185 Luc activity of PTC124 but found that, while G418 exhibits varying activity in every read-through ass

186 Furthermore, treatment of SMA mice with G418 increased both SMN protein and mouse motor function

187 ately 20% of WT levels and, when paired with G418, rescued G542X-CFTR function to approximately 50% o

188 to C57BL/6 ES cells and clones selected with G418 followed by injection into Balb/c blastocysts.

189 ell clones expressing RFP were selected with G418 sulfate and individual clones were isolated.

190 s, and resistant colonies were selected with G418.

191 CREF cells were generated by selection with G418 and compared with respect to transformed properties

192 ression construct followed by selection with G418 for 10-14 days.

193 After selection with G418, Bax, Bcl-2 and pCI-neo stably transfected cells we

194 rovirus infection followed by selection with G418.

195 ectants were obtained through selection with G418.

196 ection of LZRN-transduced cells in situ with G418.

197 es, initiated from testes, were treated with G418 to eliminate the somatic cells and select for the p

198 sion PC-3 clones were selected in vitro with G418, which were then combined and injected s.c. in nude