ライフサイエンスコーパス: GTF

1 Mutagenesis of Asp413 to Thr resulted in a GTF which expressed only about 12% of the wild-type acti

2 caffolds or even chromosomes, and includes a GTF/GFF viewer to visualize and manage annotation files.

3 HA) or buffer, and subsequently exposed to a GTF solution.

4 imulation of TBP binding requires additional GTFs, including TFIIB and Srb4.

5 Compared with GTF in solution, adsorbed GTF displayed activity over a much wider range of pH val

6 shed by formation of glucans by the adsorbed GTF.

7 WSHA was more effective in adsorbing GTF than was naked HA.

8 aluminum hydroxide (alum) with buffer, alum-GTF, or alum-GTF together with either Escherichia coli D

9 s also observed after administration of alum-GTF with the P. gingivalis DNA either together or separa

10 roxide (alum) with buffer, alum-GTF, or alum-GTF together with either Escherichia coli DNA, Fusobacte

11 trary to expectation, animals receiving alum-GTF plus bacterial DNA (P. gingivalis in particular) dem

12 GTF compared to animals immunized with alum-GTF alone.

13 gg, increased the amount of GTF activity and GTF antibody-reactive protein ca. fivefold but did not c

14 ae, we perturb Pol II catalytic activity and GTF function and analyze their effects on TSS usage geno

15 hanced the immunological response to CAT and GTF epitopes, but also extended the protective effect of

16 nct domains, also found in GTF180-DeltaN and GTF-SI glucansucrases of glycoside hydrolase family 70.

17 SOCS]), the effects of P. gingivalis DNA and GTF exposure on rat splenocyte production of SOCS family

18 mooth-surface molar caries in the GBP59- and GTF-immunized S. mutans-infected groups were each signif

19 After 71 days of infection, GBP59- and GTF-injected groups had smaller numbers of S. mutans on

20 weeks after the second injection, GBP59- and GTF-injected rats contained significant levels of saliva

21 ct to induce antibody reactive with GbpB and GTF native proteins, rats were injected subcutaneously w

22 yte proliferative responses to CAT, GLU, and GTF were observed after coimmunization with CAT-GLU comp

23 load increased both GOP and SBR, and GOP and GTF were correlated (P < 0.001), suggesting that the two

24 MAP kinase activation stimulated Pol II and GTF promoter binding.

25 moter bound Pol II in addition to Pol II and GTF recruitment.

26 provide conversion utilities for the VCF and GTF formats.

27 e synthesis of the glucans to which GbpA and GTFs can bind and which promote S. mutans attachment to

28 ator, histone acetyltransferases (HATs), and GTFs onto immobilized chromatin and naked DNA templates

29 However, the polymerase and GTFs were subsequently lost from the promoter in cells e

30 pression of several genomic islands, such as GTF-B/C, TnSmu, CRISPR1-Cas and CRISPR2-Cas, was found t

31 We are able to associate GTFs to nearly half of all putative promoters and show e

32 gnition of S. mutans cell surface-associated GTF by specific antibodies but had no effect on antibodi

33 Oral bacterial GTFs use sucrose as a substrate in synthesis of either w

34 ental caries can be induced to peptide-based GTF vaccines after mucosal administration if combined wi

35 The majority of glucan made by GTF adsorbed to parotid saliva-coated HA remained attach

36 r-insoluble glucan synthesis from sucrose by GTF was noted at a concentration as low as 7.8 microgram

37 p in our understanding of gene regulation by GTFs for all three domains of life and provides an examp

38 ng immunization and were analyzed for anti-C-GTF activity by enzyme-linked immunosorbent assay.

39 The levels of immunoglobulin A (IgA) anti-C-GTF activity in the nasal wash from both groups after im

40 Plasma IgG anti-C-GTF activity was highest in mice immunized by the i.n. r

41 The IgA1 anti-C-GTF response in nasal wash samples for liposomal antigen

42 a, and vaginal immunoglobulin A (IgA) anti-C-GTF responses and higher levels of plasma IgG anti-C-GTF

43 IgG and IgA anti-C-GTF responses in serum were detected on day 14.

44 en and MPL-AF had higher salivary IgA anti-C-GTF responses than mice immunized with antigen plus MPL-

45 Salivary IgA anti-C-GTF responses were induced to a lesser extent.

46 onses and higher levels of plasma IgG anti-C-GTF than the orally immunized groups.

47 n preparation rich in glucosyltransferase (C-GTF) from Streptococcus mutans, alone or in liposomes.

48 tococcus mutans crude glucosyltransferase (C-GTF) with or without MPL-AF added to the vaccine or inco

49 and systemic immune responses to liposomal C-GTF following i.n. immunization.

50 These results show that C-GTF vaccines were more effective in inducing a local sec

51 tion resulted in an anamnestic response to C-GTF resulting in 10- and 100-fold increases in saliva an

52 idic linkages, whereas the Spp- strain CH107 GTF produced primarily alpha1,6-linked glucans.

53 ng protein-coimmunoprecipitation, ChIP-Chip, GTF perturbation and knockout, and measurement of transc

54 they mediate the recruitment of many common GTFs.

55 ization and were assayed by ELISA for anti-E-GTF activity.

56 Significantly higher anti-E-GTF responses were detected in saliva and nasal wash sam

57 glucosyltransferase-enriched preparation (E-GTF) administered by nasal or tonsillar topical spray.

58 can-synthesizing glucosyltransferase enzyme (GTF-I) from Streptococcus mutans and thioredoxin from Es

59 s streptococcal glucosyltransferase enzymes (GTF) can provide immunity to dental caries infection.

60 (Taspase1) cleaves a ubiquitously expressed GTF (TFIIA) to enable tissue-specific (testis) transcrip

61 4RPE all had similar levels of extracellular GTF activity, strain CH107 had less than 15% of the pare

62 indicated that the amounts of extracellular GTF protein in all four strains were similar.

63 fferences in a general transcription factor (GTF) binding site motif across several data sets.

64 se II (pol II)-general transcription factor (GTF) complexes are unstable, an assembly of pol II with

65 subunit of the general transcription factor (GTF) TFIIH, drives scanning through its DNA-dependent AT

66 polymerase II general transcription factor (GTF) that has also been implicated in the mechanism of a

67 n of multiple general transcription factors (GTFs) and RNA polymerase II (Pol II).

68 ining all the general transcription factors (GTFs) and the other pol II.

69 licity of generalized transcription factors (GTFs) are required.

70 and conserved general transcription factors (GTFs) assembles and opens DNA upstream of TSSs.

71 zymes and the general transcription factors (GTFs) by activators.

72 (Pol II), and general transcription factors (GTFs) initially occurred at the inducible PPARgamma2 pro

73 e II (Pol II) general transcription factors (GTFs) that are thought to be critical for formation of p

74 teins and the general transcription factors (GTFs) that assemble at TATA-containing promoters.

75 ctivities and general transcription factors (GTFs) to promoters.

76 f a number of general transcription factors (GTFs), all of which have been either cloned or purified

77 of HSFs with general transcription factors (GTFs), as has been described for numerous other transcri

78 ghly purified general transcription factors (GTFs), for differential core promoter-dependent transcri

79 h contain the general transcription factors (GTFs), RNA polymerase II (Pol II), and coactivators.

80 including the General Transcription Factors (GTFs), RNA polymerase II (RNA pol II), co-activators, co

81 ssue-specific general transcription factors (GTFs), such as testis-specific TBP-related factor 2 (TRF

82 se II and its general transcription factors (GTFs).

83 (RNAPII) and general transcription factors (GTFs).

84 y the loss of general transcription factors (GTFs).

85 ) dissociates general transcription factors (GTFs; TFIIA, TFIIB, TBP, TFIIE, TFIIF, and TFIIH) and es

86 x comprising 20 subunits, is among the first GTFs to bind the core promoter.

87 F) or its descendants, gene transfer format (GTF) and GFF3.

88 tein-coding and lncRNA gene transfer format (GTF) annotations to generate enhanced annotations that t

89 erence sequence(s), a Gene Transfer Format (.GTF) file with CDS information and a SNP report(s) in an

90 intermediate in assembly, consisting of four GTFs and promoter DNA, could be isolated and supplemente

91 Sera from GTF-immunized rats, assessed for binding to linear pepti

92 for at least 7 of the possible 42 functional GTF pairs.

93 Generalized transfer functions (GTFs) are available to compute the more relevant central

94 0 to -80 Torr, while transfer function gain (GTF) was calculated to assess the linear dynamic relatio

95 PhyloCSF++ annotates coding sequences in GFF/GTF files using precomputed tracks or computes and score

96 Glucosyltransferase (GTF) enzymes of mutans streptococci are considered virul

97 The enzyme glucosyltransferase (GTF) produced by mutans streptococci is the key factor i

98 ain (GLU) of the enzyme glucosyltransferase (GTF), which is an important virulence factor of Streptoc

99 nd vicinity with GBP59, glucosyltransferase (GTF), or phosphate-buffered saline (sham injection), eac

100 of Streptococcus mutans glucosyltransferase (GTF).

101 d kinetic properties of glucosyltransferase (GTF) adsorbed onto hydroxyapatite (HA) surfaces.

102 inding (GLU) domains of glucosyltransferase (GTF) of mutans streptococci has resulted in enhanced lev

103 ed strong inhibition of glucosyltransferase (GTF), in vitro adherence and glucan-induced agglutinatio

104 Streptococcus sobrinus glucosyltransferase (GTF), an enzyme involved in dental caries pathogenesis,

105 Glucans produced by the glucosyltransferase (GTF) of Streptococcus gordonii confer a hard, cohesive p

106 aliva that inhibits the glucosyltransferase (GTF) of Streptococcus mutans, a virulence enzyme involve

107 f Streptococcus mutans glucosyltransferases (GTF) have been shown to induce protective immunity in Sp

108 m mutans streptococcal glucosyltransferases (GTF) and glucan binding protein B (GbpB).

109 Mutans streptococcal glucosyltransferases (GTF) have been demonstrated to be effective components o

110 Glucosyltransferases (GTFs) are key virulence factors in this process, as they

111 enzymatic activity of glucosyltransferases (GTFs) of the mutans group streptococci.

112 acid sequences of the glucosyltransferases (GTFs) of mutans streptococci with those from the alpha-a

113 l-terminal domain with glucosyltransferases (GTFs), the enzymes responsible for catalyzing the synthe

114 onfer specificity for one or more homologous GTF proteins.

115 However, GTFs are population averages and therefore may not adapt

116 o the binding modes of previously identified GTF inhibitors.

117 produced via the Fenton reaction inactivate GTF, a factor in the production of dental caries.

118 This response inhibited GTF enzyme function.

119 ylase complex which is capable of inhibiting GTF and may contribute to control of S. mutans colonizat

120 munization as a simple alternative to intact GTF to enhance protective immunity against cariogenic mi

121 In this study, 20 20-mer linear GTF peptides were synthesized, 17 identified on the basi

122 inding of C/EBP activators, Pol II, and most GTFs preceded the interaction of SWI/SNF enzymes with th

123 owever, cross-reacting antibody to S. mutans GTF or GBP59 was not induced by the respective antigen.

124 ruct also inhibited the ability of S. mutans GTF to synthesize glucan.

125 nd glucan-binding (GLU) domains of S. mutans GTF-I encoded by gtfB.

126 and 1183 to 1473, respectively, of S. mutans GTF-I.

127 e inciting peptide and with intact S. mutans GTF.

128 water-soluble glucan synthesis by S. mutans GTFs (P < 0.0001 and P < 0.05, respectively).

129 hat calculated for the GBDs of two S. mutans GTFs, one of which catalyzes the synthesis of water-solu

130 T-cell proliferation) was observed to native GTF following MAP 11 (amino acids 847 to 866; VVINNDKFVS

131 y that elicited immunoreactivity with native GTF and demonstrated protection against dental caries in

132 The ability of GTF to synthesize extracellular glucans was inhibited by

133 The activities of GTF adsorbed to HA and that remaining in solution were m

134 o be involved with the catalytic activity of GTF.

135 adsorbed to HA was reduced by adsorption of GTF to the same surface and was almost completely abolis

136 ry determinant, rgg, increased the amount of GTF activity and GTF antibody-reactive protein ca. fivef

137 functional significance and conservation of GTF primary structure among enzyme isoforms.

138 her Gly or Ala resulted in enzymes devoid of GTF activity, indicating the essential nature of these t

139 22-mer sequence from the catalytic domain of GTF.

140 r sequence from the glucan binding domain of GTF.

141 insoluble and recombinantly expressed GLU of GTF and that this construct was especially effective in

142 bodies to the glucan-binding region (GLU) of GTF by an enzyme-linked immunosorbent assay.

143 l insights into the inhibitory mechanisms of GTF-targeting compounds, strengthening their potential a

144 had previously selected peptide subunits of GTF for vaccine development based on putative functional

145 Truncated variants of GTF-I from Streptococcus downei MFe28 were purified by m

146 odies are capable of inhibiting a variety of GTF activities.

147 als a dynamic state of TFIIH, the largest of GTFs, in PIC/ITC with distinct functional consequences a

148 ng of the RNR3 promoter requires a number of GTFs, mediator and RNA polymerase II.

149 n alteration in Pol II catalytic activity or GTF function.

150 ) and salivary IgA antibody levels to CAT or GTF than rats immunized with either construct alone.

151 otective immunity induced by either GBP59 or GTF appears to result from antibodies to independent epi

152 t sufficient, in the context of all purified GTFs and RNA polymerase II, to mediate transcription syn

153 R analyses indicated that partially purified GTFs from the Spp+ strains CH1, CH2RPE, and CH4RPE all p

154 TFIIA (DA) recruits pol II and the remaining GTFs to a model promoter in vitro.

155 are unstable, an assembly of pol II with six GTFs and promoter DNA could be isolated in abundant homo

156 14-day intervals with peptides, S. sobrinus GTF, or phosphate-buffered saline.

157 m the communized group inhibited S. sobrinus GTF-mediated insoluble glucan synthesis in vitro above t

158 ized as above or with Streptococcus sobrinus GTF and then infected with S. sobrinus to explore the ef

159 ibited the ability of Streptococcus sobrinus GTF to synthesize insoluble glucan.

160 lucan synthesis by S. mutans and S. sobrinus GTFs (P < 0.0001 and P < 0.05, respectively).

161 ants, also bound intact mutans streptococcal GTF in an enzyme-linked immunosorbent assay and inhibite

162 inciting antigen, with mutans streptococcal GTFs, and with a 21-mer peptide (CAT) containing an aspa

163 Targeting GTFs has emerged as a promising strategy for preventing

164 A subset of A. thaliana GTFs have also expanded in number, indicating that GTF d

165 imated central BP with greater accuracy than GTFs in the low PP amplification group (e.g., central sy

166 Temperature-activity profiles indicated that GTF adsorbed to surfaces had a lower temperature optimum

167 ave also expanded in number, indicating that GTF diversification and expansion is a general phenomeno

168 This indicates that GTFs remain associated with pol II much longer than prev

169 The GTF-inhibiting factor (GIF) was initially identified as

170 tions in the region of gtfG that encodes the GTF carboxyl terminus were characterized.

171 constructs from the CAT or GLU region of the GTF of mutans streptococci or coimmunized with a combina

172 the original data that helped popularize the GTF.

173 to induce mucosal antibody in the rat to the GTF peptide vaccines HDS and HDS-GLU after intranasal ad

174 ition of the GbpB-derived SYI peptide to the GTF-derived CAT peptide construct not only enhanced the

175 site-directed mutagenesis approach with the GTF-I enzyme of Streptococcus mutans GS-5, we identified

176 nd that, against expectations, TFIID and the GTFs are not sufficient.

177 n system containing TFIID, Mediator, and the GTFs.

178 the mechanisms of catalysis proposed for the GTFs and related enzymes.

179 t of diverse molecular players including the GTFs, the Mediator complex, and DNA repair factors that

180 present in the glucan-binding domain of the GTFs had little overall effect on enzymatic activity, al

181 iption complex containing RNA and all of the GTFs is unstable and is susceptible to extensive backtra

182 of the postinitiation complexes retained the GTFs when pol II was stalled at position +27 relative to

183 Here we show that the GTFs IIA, IIB, IIF, and IIE are required for efficient R

184 Promoter DNA was associated only with the GTFs, suspended above the pol II cleft and not in contac

185 support the functional significance of these GTF domains in dental caries pathogenesis and present co

186 among the activator proteins and these three GTFs were not detected with other transcription factors,

187 ns revealed their strong binding affinity to GTF.

188 vels of antibody to the CAT construct and to GTF.

189 of serum immunoglobulin G (IgG) antibody to GTF or CAT in the CAT-GLU group were significantly great

190 s, suggesting that GIF specifically binds to GTF on S. mutans.

191 cts showed proliferation of T lymphocytes to GTF.

192 ry IgA antibody, and T-cell proliferation to GTF compared to animals immunized with alum-GTF alone.

193 arly in this study, the enhanced response to GTF after immunization with the CAT-GLU construct result

194 d in serum IgG and salivary IgA responses to GTF and CAT which were greater than after coimmunization

195 domains of Fos and Jun abolished binding to GTFs, although the presence of DNA was not required for

196 A similar effect was observed when GTF was adsorbed to naked HA from a mixture with lysozym

197 aliva; however, no enhancement was seen when GTF was adsorbed from a mixture with albumin.

198 ion complex, but little is known about which GTFs other than TBP are required.

199 Compared with GTF in solution, adsorbed GTF displayed activity over a

200 induce immune responses which interfere with GTF-mediated glucan synthesis in vitro, and can protect

201 induce immune responses which interfere with GTF-mediated glucan synthesis.

202 nvolved in protein-protein interactions with GTFs: one is the repressor domain (RD) located in the N-