ライフサイエンスコーパス: Gag protein

1 sm of the membrane targeting domain of HIV-1 Gag protein.

2 d with the amphotropic envelope or the glyco-Gag protein.

3 particles are assembled from the multidomain Gag protein.

4 from the human immunodeficiency virus (HIV) Gag protein.

5 ecombinant vaccinia virus expressing the HIV Gag protein.

6 ractions capable of reversibly extending the Gag protein.

7 A), nucleocapsid protein (NC), or entire DP6-Gag protein.

8 ecombinant vaccinia virus expressing the HIV Gag protein.

9 ized "buttons" containing oligomerized viral Gag protein.

10 large areas of the VLP membrane are void of Gag protein.

11 cytosolic immature and endocytic mature HIV gag protein.

12 C terminus of HIV p17 matrix within the HIV gag protein.

13 found in the equine infectious anemia virus Gag protein.

14 rovirus particle assembly is mediated by the Gag protein.

15 oviruses and expresses aberrant forms of the Gag protein.

16 d region within the CA and NC regions of the Gag protein.

17 combined with antibody staining for the HIV Gag protein.

18 treatment of cells expressing the wild-type Gag protein.

19 s toxoid precursor, EBV nuclear Ag 2, or HIV gag protein.

20 orphisms present in the CA-SP1 region of the Gag protein.

21 and stabilization of the RNA dimer requires Gag protein.

22 domain of human immunodeficiency virus (HIV) gag protein.

23 multiple biochemical activities of the viral Gag protein.

24 n of the feline immunodeficiency virus (FIV) Gag protein.

25 V particles, a process directed by the viral Gag protein.

26 human immunodeficiency virus type 1 (HIV-1) Gag proteins.

27 a role in PM targeting of several retroviral Gag proteins.

28 is critical for PM association of retroviral Gag proteins.

29 emonstrate that SPTBN1 associates with HIV-1 gag proteins.

30 cleocytoplasmic transport properties of both Gag proteins.

31 of the mutational correlation matrix of HIV Gag proteins.

32 ate nucleocapsid domains of assembling viral Gag proteins.

33 gnate nucleocapsid (NC) domains of the viral Gag proteins.

34 iruses in human genome today have functional Gag proteins.

35 ation, we created viruses bearing NES mutant Gag proteins.

36 tein products contain homology to retroviral Gag proteins.

37 e representative Beta- and Epsilonretrovirus Gag proteins.

38 s with the MA domains of the viral precursor Gag proteins.

39 porating 10% Gag-SNAP, 10% Gag-Halo, and 80% Gag proteins.

40 (gag), which recognize the virus encoded p24(gag) protein.

41 gag), the precursor for internal structural (Gag) proteins.

42 the nucleocapsid (NC), spacer 2 (SP2), or p6(Gag) proteins.

43 in the position of NC was a nonmyristoylated Gag protein able to multimerize.

44 membrane, their assembly rate accelerated as Gag protein accumulated in cells, and typically 5-6 min

45 of an MLV vector, we hypothesized that other Gag proteins act cooperatively with p12 during the early

46 RSV Gag protein adopts a more tightly curved lattice than is

47 with both the encoded Matrix (MA) domain of Gag protein and 5' UTR of the translating mRNA and promo

48 rNDV vector expressing a codon-optimized HIV Gag protein and demonstrated its ability to induce a Gag

49 We have purified Rous sarcoma virus (RSV) Gag protein and in parallel several truncation mutants o

50 human immunodeficiency virus type 1 (HIV-1) Gag protein and that their interaction is important for

51 efore, understanding the processing of HIV-1 Gag protein and the resulting epitope repertoire is esse

52 ions in the steady-state levels of the HIV-1 Gag protein and virus production; Mov10 was efficiently

53 ble by SEM and compared these numbers for WT Gag proteins and for Gag proteins that were blocked at t

54 escape, varies widely even in the conserved Gag proteins and suggest that differential escape costs

55 Western blot assays using recombinant viral Gag protein, and an indicator cell line that can detect

56 with broad CD8(+) T cells that targeted the Gag protein, and CD8(+) T cells from these individuals e

57 detection of gagpol mRNA, intracellular p24 Gag protein, and cell surface markers.

58 ognition of discrete epitopes largely in the Gag protein, and expansion of IL-21(+) CD4(+) T cells in

59 human immunodeficiency virus type 1 (HIV-1) Gag protein, and the same mRNAs were enriched in MLV and

60 sition as well as levels of IAP transcripts, Gag proteins, and reverse transcription products.

61 linked target sites in the transmitted virus Gag protein are already adapted, and that this transmitt

62 oxic T lymphocytes [CTLs]) against the HIV-1 Gag protein are associated with control among those with

63 T lymphocyte (CTL) responses against the HIV Gag protein are associated with lowering viremia; howeve

64 ing and high-affinity binding to the cognate Gag protein are exposed in helical junctions.

65 In these particles, the Gag proteins are arranged radially as elongated rods.

66 perimental results strongly suggest that RSV Gag proteins are capable of interacting within the nucle

67 for the heterologous RNA copackaging; HIV-1 Gag proteins are capable of mediating HIV-1 and HIV-2 RN

68 virus (RSV) and murine leukemia virus (MLV) Gag proteins are selectively recruited to these structur

69 In vitro, recombinant Gag proteins are soluble but assemble into virus-like pa

70 Retroviral Gag proteins are synthesized as soluble, myristoylated p

71 For Rous sarcoma virus (RSV), Gag proteins are synthesized in the cytoplasm and then u

72 1) replication, newly synthesized retroviral Gag proteins are targeted to lipid raft regions of speci

73 of retroviral replication, newly synthesized Gag proteins are targeted to the plasma membrane (PM), w

74 1) replication, newly synthesized retroviral Gag proteins are targeted to the plasma membrane of most

75 rus (RSV) capsid protein is cleaved from the Gag protein as the proteolytic intermediate CA-SP.

76 proteins that came in close proximity to the Gag proteins as they were synthesized, transported, asse

77 approaches have illuminated the dynamics of Gag protein assembly, viral RNA packaging and ESCRT (end

78 esults suggest a multistep pathway for HIV-1 Gag protein assembly.

79 ering with proteolytic cleavage of the viral Gag protein at a specific site.

80 ar export, thereby isolating these "trapped" Gag proteins at an early assembly step.

81 constructed a series of models of the mutant Gag protein based on these domain structures, and tested

82 Confocal imaging of wild-type and mutant Gag proteins bearing different fluorescent tags suggeste

83 like particles produced by the expression of Gag proteins bearing p10 mutations were also aberrant, w

84 oteins colocalized extensively with chimeric Gag proteins bearing the same CA domain, implying that G

85 proteins, but, surprisingly, only the mutant Gag protein biotinylated the HIV-1 Envelope protein.

86 able to study how monovalent and multivalent GAG.protein bonds respond to directed mechanical forces.

87 he Gag-Pro-Pol precursor encodes most of the Gag protein but now includes the viral enzymes: protease

88 s encoded by the HIV-1 pol gene and neighbor Gag proteins, but, surprisingly, only the mutant Gag pro

89 ost conserved segment in the highly variable Gag protein called the major homology region (MHR).

90 HIV Gag protein can be directly detected in infected resting

91 Purified retroviral Gag proteins can assemble in vitro to form immature viru

92 ompletely cleaved) CA and that CA-containing Gag proteins can be cleaved by the viral protease in SIV

93 sfection system in which epitopically tagged Gag proteins can be traced back to their mRNA origins in

94 thogens, we examined whether HIV-1 and HIV-2 Gag proteins can coassemble and functionally complement

95 cts were alleviated by the coexpression of a Gag protein carrying a wild-type (WT) NC domain but devo

96 th a detection limit of 0.5-1 Gagpol mRNA(+)/Gag protein(+) cells per million CD4 T cells.

97 primer binding site through a portion of the gag protein coding region.

98 However, wild-type Gag proteins colocalized extensively with chimeric Gag p

99 Truncated Gag proteins comprising the MA, CA, and SP domains (MACA

100 rap assay, based on co-expression of the HIV Gag protein, confirmed that this post-translational modi

101 Many retroviral Gag proteins contain PPXY late assembly domain motifs th

102 Retroviral Gag proteins contain short late-domain motifs that recru

103 truncating the CT or by using a chimeric MLV Gag protein containing the HIV-1 MA without fully restor

104 n efficiency for each of the three telomeric Gag proteins correlates with the relative abundance of t

105 tion experiments revealed that the wild-type Gag protein could partially rescue export-defective Gag

106 In contrast, a single natural Gag protein covered only 37% (9 of 9) and 67% (8 of 9).

107 ion at early stages of infection, proceeding Gag protein detection.

108 The Capsid region of the viral Gag protein dictates susceptibility to MX2, and the bloc

109 ization of BLT mice with the conserved viral Gag protein did not result in detectable prechallenge re

110 Retroviral Gag proteins direct virus particle assembly from the pla

111 The mouse mammary tumor virus (MMTV) Gag protein directs the assembly in the cytoplasm of imm

112 l genomic RNAs are encapsidated by the viral Gag protein during virion assembly.

113 f HIV-1 infection with IgG Abs against HIV-1 Gag proteins (e.g., p24) and/or production of IgG2 Abs a

114 NHP were immunized with HIV Gag protein emulsified in Montanide ISA 51, an oil-based

115 Rhesus macaques (RMs) were primed with SIV Gag protein emulsified in Montanide ISA51 with or withou

116 ractions between the RNA dimer and the viral Gag protein ensure selective packaging into nascent imma

117 The retroviral Gag protein exhibits extensive amino acid sequence varia

118 human immunodeficiency virus type 1 (HIV-1) Gag protein expressed in COS-1 cells using fluorescence

119 portion of the simian immunodeficiency virus Gag protein expressed in mice by an L1-modified rMRV was

120 silencing the m(6)A writers decreased HIV-1 Gag protein expression in virus-producing cells, while s

121 in vivo experiments revealed that higher HIV Gag protein expression positively correlates with an enh

122 losis vaccine strains expressing SIV Env and Gag proteins, followed by systemic heterologous (MVA-SIV

123 vectors encoding several RSV/HIV-1 chimeric Gag proteins for expression in either insect cells or ve

124 his model, we took advantage of a retroviral Gag protein from the prototypic foamy virus (PFV) that i

125 factor (Rej) necessary for the synthesis of Gag protein from unspliced viral RNA.

126 to study the cytoplasmic interactions of the Gag proteins from human immunodeficiency virus type 1 (H

127 analyzed the nuclear trafficking ability of Gag proteins from six retroviral genera.

128 Gag proteins from two other retroviruses gave similar re

129 types having AKV Env and Moloney MLV (MoMLV) Gag proteins, further indicating that AKV Env sequence v

130 was administered alone or together with HIV Gag protein (Gag), and the magnitude, quality, and pheno

131 ically detected a tetracysteine-tagged HIV-1 Gag protein (Gag-TC) in HeLa, Mel JuSo, and Jurkat T cel

132 HIV-1 Gag protein gains access to ESCRT directly by binding Al

133 virus (MuLV) that utilizes its glycosylated Gag protein (gGag) to evade APOBEC3.

134 ce, we demonstrate that the MLV glycosylated Gag protein (glyco-Gag) enhances viral core stability.

135 lycans (PGs), a family of glycosaminoglycan (GAG)-protein glycoconjugates, contribute to animal physi

136 further suggesting that, compared to NC, the Gag protein has a greater propensity to affect RNA struc

137 Previously, no retroviral Gag protein has been highly purified in milligram quanti

138 The Gag protein has been shown to interact with other Gag pr

139 For HIV-1, the Gag protein has the role of a polyprotein precursor that

140 4+ T cell immunity relative to high doses of gag protein, HIV gag plasmid DNA, or recombinant adenovi

141 ngest association with response to the HIV-1 Gag protein: HLA-B alleles known to be associated with d

142 eptide 1 (SP1), the portion of the precursor Gag protein immediately C terminal to the CTD.

143 APOBEC3B specifically interact with the IAP Gag protein in co-expressing cells, and induce extensive

144 n to interact and co-localize with the HIV-1 Gag protein in infected cells.

145 Expression of a retroviral Gag protein in mammalian cells leads to the assembly of

146 es when used as vaccine adjuvants with HIV-1 Gag protein in mice.

147 e that CCDC8, derived from a retrotransposon Gag protein in placental mammals, exclusively localized

148 human immunodeficiency virus type 1 (HIV-1) Gag protein in solution adopts compact conformations.

149 aM co-localizes and interacts with the HIV-1 Gag protein in the cytosol of infected cells.

150 rities between these host proteins and viral Gag protein in the nature of their PI(4,5)P2 interaction

151 , we detected HERVK viral-like particles and Gag proteins in human blastocysts, indicating that early

152 s producing these mutants showed assembly of Gag proteins in large, flat or dome-shaped patches at th

153 mmunodeficiency virus [FIV]) vis-a-vis their Gag proteins in live cells.

154 with a role for the MA domain of retroviral Gag proteins in modulating nucleic acid binding and chap

155 tion and the appearance of new ubiquitinated Gag proteins in virions.

156 ciated with increased in vitro expression of Gag protein, in vivo expression of Gag mRNA, and enhance

157 enabling detection of 0.5-1 gag-pol mRNA(+)/Gag protein(+)-infected cells per million.

158 which the MA domains of different retroviral Gag proteins influence gRNA packaging, highlighting vari

159 HIV-1 Gag protein interaction with cyclophilin A (CypA) is cri

160 However, mapping GAG-protein interaction networks is challenging as these

161 dies provide a powerful approach for mapping GAG-protein interaction networks, revealing new potentia

162 owledge, class of inhibitors that target the GAG-protein interaction.

163 sights into the nature of glycosaminoglycan (GAG)-protein interactions and prove useful for optimizat

164 en shown extensively that glycosaminoglycan (GAG)-protein interactions can induce, accelerate, and im

165 Glycosaminoglycan (GAG)-protein interactions mediate critical physiological

166 GAG-protein interactions participate in neuronal develop

167 gate numerous other physiologically relevant GAG-protein interactions.

168 le method for analyzing the nanomechanics of GAG.protein interactions at the level of single GAG chai

169 finding to humans, we introduced the HIV p24 gag protein into a mAb that targets DEC-205/CD205, an en

170 membrane binding and assembly of retroviral Gag proteins into a lattice are understood.

171 HIV-1 Gag protein is a component of several experimental HIV-1

172 human immunodeficiency virus type 1 (HIV-1) Gag protein is a major target antigen for cytotoxic-T-ly

173 The capsid domain (CA) of the retroviral Gag protein is a primary determinant of Gag oligomerizat

174 The viral Gag protein is believed to chaperone tRNA(3)(Lys) placem

175 ar transport of the Rous sarcoma virus (RSV) Gag protein is intrinsic to the virus assembly pathway.

176 The matrix domain (MA) of HIV-1 Gag protein is N-myristoylated and plays an important ro

177 e N-terminal matrix (MA) domain of the HIV-1 Gag protein is responsible for binding to the plasma mem

178 The HIV-1 Gag protein is responsible for genomic RNA (gRNA) packag

179 Since the Gag protein is the central component for the production

180 The Gag protein is the main retroviral structural protein, a

181 The Gag protein is the major structural determinant of retro

182 erved that the coassembly of HIV-1 and HIV-2 Gag proteins is not required for the heterologous RNA co

183 her complicated when group-specific antigen (Gag) protein is expressed, because a significant portion

184 er, some simian immunodeficiency virus (SIV) Gag proteins lack this consensus sequence, yet still bin

185 HIV-1 particles constructed from recombinant Gag proteins lacking residues 16-99 and the p6 domain as

186 human immunodeficiency virus type 1 (HIV-1) Gag protein (lacking myristate at its N terminus and the

187 When recombinant HIV-1 Gag protein (lacking myristate at its N terminus and the

188 vitro, RNA aptamers raised against an HIV-1 Gag protein, lacking the N-terminal myristate and the C-

189 stimulate HIV-1 release but in this case the Gag protein lacks a PPXY motif, suggesting that NEDD4L m

190 Expression of the retroviral Gag protein leads to formation of virus-like particles i

191 immature capsid and domains of its component Gag proteins, less is known about the sequence of events

192 radation, resulting in reduced intracellular Gag protein levels; this phenotype was rescued by reintr

193 ching (FRAP) revealed that the population of Gag proteins localized within YB-1 complexes was relativ

194 NPO3-mediated nuclear trafficking of the RSV Gag protein may lead to a deeper appreciation for whethe

195 that phosphorylation of these CA-containing Gag proteins may require an environment that is unique t

196 The MA domain of the retroviral Gag protein mediates interactions with the plasma membra

197 The MA domain of retroviral Gag proteins mediates association with the host cell mem

198 virions shows that in these particles, HIV-1 Gag protein molecules are rod shaped.

199 nd its effects upon the interactions between Gag protein molecules in solution.

200 Animals vaccinated with HIV Gag protein/Montanide and CpG ODN or the TLR7/8 agonist

201 Gag proteins must extensively multimerize during the for

202 tags suggested that complementation between Gag proteins occurred in the nucleus.

203 hly conserved motif that is found within the Gag protein of all orthoretroviruses and some retrotrans

204 hen it is transplanted into the heterologous Gag protein of equine infectious anemia virus (EIAV).

205 Membrane binding by the Gag protein of HIV-1 and most other lentiviruses is depe

206 The myristylated full-length Gag protein of HIV-1 was purified to monodispersity.

207 ector of human serotype 5 (AdHu5) expressing Gag protein of HIV-1, in the presence or absence of pree

208 ic cell (DC)-directed LV system encoding the Gag protein of human immunodeficiency virus (HIV) (LV-Ga

209 The Gag protein of human immunodeficiency virus type 1 (HIV-

210 The Gag protein of human immunodeficiency virus type 1 direc

211 s a cellular protein that interacts with the Gag protein of Mason-Pfizer monkey virus.

212 We now describe the properties of the Gag protein of Moloney murine leukemia virus (MLV), a ga

213 the identification of a novel domain in the Gag protein of Moloney murine leukemia virus (MoLV) that

214 Before membrane transport, the multidomain Gag protein of Rous sarcoma virus (RSV) undergoes import

215 sus monkey rhadinovirus (RRV) expressing the Gag protein of SIVmac239 was constructed in the context

216 The Gag protein of the murine retrovirus mouse mammary tumor

217 dition, we observed that in cells expressing Gag proteins of both viruses, HERV-K(CON) Gag colocalize

218 Altogether, these results indicate that Gag proteins of endogenous retroviruses can coassemble w

219 We show that 1) ORF1 and Gag proteins of retrotransposons contain high amounts of

220 The Gag proteins of Rous sarcoma virus (RSV) and human immun

221 ent, ALIX-binding late domains within the p6(Gag) proteins of SIV(mac239) ((40)SREKPYKEVTEDLLHLNSLF(5

222 insertions/substitutions are observed in the Gag-protein of HIV-1 variants resistant to HIV-1 proteas

223 To examine the effect of Gag protein on HIV-1 RNA transport, we analyzed the cyto

224 Localization of the HIV type-1 (HIV-1) Gag protein on the plasma membrane (PM) for virus assemb

225 ys residues showed that the contacts between Gag proteins on the membrane are similar to the known co

226 determined that despite encoding a truncated Gag protein, only the full-length Gag protein was incorp

227 The HIV-1 Gag protein orchestrates all steps of virion genesis, in

228 A subgenomic Ty1 mRNA encodes a truncated Gag protein (p22) that is cleaved by Ty1 protease to for

229 How the Gag proteins pack together in the lattice is incompletel

230 nism, referred to as cis packaging, in which Gag proteins package the RNA from which they were transl

231 In accordance with the model that Gag protein pairing triggers assembly, we found that cys

232 The Gag protein plays a crucial role in the assembly of retr

233 After priming, RMs that received SIV Gag protein plus poly-IC developed significantly higher

234 ignificantly increased in RM primed with SIV Gag protein plus poly-IC, with or without the TLR7/8 lig

235 strate that prime-boost vaccination with SIV Gag protein/poly-IC improves magnitude, breadth, and dur

236 MA) that lies at the N-terminus of the viral Gag protein precursor appears to be one of the crucial s

237 occurs prior to encapsidation and that HIV-2 Gag proteins primarily package one dimeric RNA rather th

238 llenge was not significantly enhanced by SIV Gag protein priming with any of the adjuvants.

239 ) T cell response in BAL was enhanced by SIV Gag protein priming with poly-IC or CpG, which correlate

240 icing phenotype, we show that the effects on Gag protein processing and virus particle production of

241 hown to affect virus particle production and Gag protein processing.

242 nd Epsilonretrovirus Gag proteins, the other Gag proteins produced VLPs as confirmed by TEM, and morp

243 tes NF-kappaB, significantly augmented viral Gag protein production in XMRV-infected cells.

244 the nuclear export mutants, we asked whether Gag protein-protein interactions occur within the nucleu

245 mediated by the myristoylated N terminus of Gag, protein-protein interactions between CA domains, an

246 human immunodeficiency virus type 1 (HIV-1) Gag protein recruits Tsg101 to facilitate HIV-1 particle

247 as severely diminished even though NC mutant Gag proteins retained the ability to assemble spherical

248 release from 293T cells, although NC mutant Gag proteins retained the ability to interact with cellu

249 Since NC mutant Gag proteins retained the interaction with Tsg101, we co

250 ire the coordinated functioning of the viral Gag protein's multiple biochemical activities.

251 the role(s) of the alternative ORF2-derived gag protein(s) of BM5def in viral pathogenesis.

252 tion inhibitors are novel as they target the Gag protein, specifically by inhibiting CA-SP1 proteolyt

253 ag) method is 1,000-fold more sensitive than Gag protein staining alone, with a detection limit of 0.

254 l-length JSRV expression construct abolished Gag protein synthesis and released viruses in 293T cells

255 pacts on FRET between normally myristoylated Gag proteins than do CA-CTD mutations.

256 The only Gag protein that displayed CRM1-dependent nuclear cyclin

257 ium in solution, and have described a mutant Gag protein that remains monomeric at high concentration

258 esistant to multiple PR inhibitors or mutant Gag proteins that confer resistance to the maturation in

259 us particles, requiring interactions between Gag proteins that form a protein layer under the viral m

260 , virus-like particles can be generated from Gag proteins that lack the N-terminal myristic acid modi

261 ed these numbers for WT Gag proteins and for Gag proteins that were blocked at the last step in buddi

262 In the Rous sarcoma virus (RSV) Gag protein, the 25 amino-acid residues of the p10 domai

263 e representative Beta- and Epsilonretrovirus Gag proteins, the other Gag proteins produced VLPs as co

264 ively, our results show that conjugating HIV Gag protein to a TLR7/8 agonist is an effective way to e

265 to monitor the binding of recombinant HIV-1 Gag protein to Cy5-tagged 190-base RNAs.

266 , extended scaffold that connects retroviral Gag proteins to ESCRT-III and other cellular-budding mac

267 Although it was traditionally thought that Gag proteins trafficked directly from the cytosol to the

268 Our results offer new insights into HIV-1 Gag protein trafficking and activities and provide new p

269 ated proteins offers new insights into HIV-1 Gag protein trafficking and activities and provides new

270 discovered that the Rous sarcoma virus (RSV) Gag protein transiently localizes to the nucleus, althou

271 at in S6-expressing cells, although Gag(Pr55(gag)) protein translation was unaffected, processing and

272 ian alpharetrovirus Rous sarcoma virus (RSV) Gag protein undergoes transient nucleocytoplasmic transp

273 OBEC3 packaging in the virion, the MLV glyco-Gag protein uses a unique mechanism to counteract the an

274 the assembly process and characterizing the Gag protein using neutron scattering, we have identified

275 unizing BLT mice against the conserved viral Gag protein, utilizing a rapid prime-boost protocol of p

276 ponses to a dendritic cell (DC)-targeted HIV gag protein vaccine in mice.

277 overy study of 364 peptides from three HIV-1 Gag protein variants.

278 d 12 h after stimulation with RMD, while p24 Gag protein was detected for the first time after 18 h p

279 truncated Gag protein, only the full-length Gag protein was incorporated into virus particles.

280 The localization of Gag proteins was examined in the wild type and in mutant

281 ference in cross-clade reactivity to Nef and Gag proteins was significant (P<.0001).

282 esis/assembly system for chimeric HIV type 1 Gag proteins, we have replicated the activity of PA-457

283 c labeling and pulse-chase analysis of HIV-1 Gag proteins, we verified that chicken BST-2 blocks the

284 en residues on capsid and matrix of the same Gag protein were often due to structural proximity.

285 flotation analyses, the intact RSV and HIV-1 Gag proteins were more similar to multimerized MA than t

286 Epitopes within Nef and Gag proteins were the most commonly recognized in both v

287 d by several thousand molecules of the viral Gag protein, which assemble to form the known hexagonal

288 human immunodeficiency virus type 1 (HIV-1) Gag protein, which directs viral assembly and release, a

289 yze the interactions of the HIV-1 structural Gag proteins, which involved tagging wild-type and mutan

290 The Gag protein with a mutation at the dimer interface also

291 is a transmembrane version of the structural Gag protein with an extra 88-amino-acid leader region th

292 alternative version of the structural viral Gag protein with an extra upstream region that provides

293 inking nuclear trafficking of the retroviral Gag protein with gRNA incorporation.

294 which involved tagging wild-type and mutant Gag proteins with a biotin ligase.

295 and protein expression levels indicated that Gag proteins with a chimeric HIV-1 CA NTD/HTLV-1 CA CTD

296 enesis, we generated and analyzed a panel of Gag proteins with chimeric HIV-1/HTLV-1 CA domains.

297 Furthermore, chimeric Gag proteins with the HTLV-1 CA NTD produced particles p

298 rotein has been shown to interact with other Gag proteins, with the viral RNA, and with the cell memb

299 bic residues resulted in the accumulation of Gag proteins within the nucleus and a budding defect gre

300 rease in fluorescence, while addition of the Gag protein yielded a large change in fluorescence, furt