ライフサイエンスコーパス: Gardnerella

1 nce of 13 different species within the genus Gardnerella.

2 naerococcus, Prevotella, and Bifidobacterium/Gardnerella.

3 tween PTB and lower Lactobacillus and higher Gardnerella abundances replicated in the low-risk cohort

4 Prevalences of Gardnerella and anaerobic gram-negative rods were signif

5 Gardnerella and Candida sensitivity remained at 100% for

6 and Gardnerella were highly exclusive, while Gardnerella and L. iners often coexisted at high frequen

7 We validate results in Gardnerella and Lactobacillus co-cultures, and in two cl

8 n the vaginal microbiome were Lactobacillus, Gardnerella, and Anaerococcus, while Lactobacillus, Anae

9 or each of the three organisms, Trichomonas, Gardnerella, and Candida, positivity at each time point

10 oints (0, 24, 48, and 72 h) for Trichomonas, Gardnerella, and Candida.

11 Gardnerella as the most abundant bacterial genus increas

12 genera commonly isolated include Aerococcus, Gardnerella, Bifidobacterium, and Actinobaculum.

13 aplasma urealyticum, Mycoplasma hominis, and Gardnerella/Bifidobacterium species.

14 in BV(+) women were Prevotella, Megasphaera, Gardnerella, Coriobacterineae, Lachnospira, and Sneathia

15 dds ratio (OR): 4.7 [1.3-16.5], p=0.017) and Gardnerella-dominant microbiome (p=0.049) were associate

16 ignificance for non-vaginalis species of the Gardnerella genus and for Pandoraea commovens in a non-c

17 The Gardnerella genus has been expanded to encompass 6 valid

18 Gardnerella had the highest overall positive rate (62%),

19 heterogeneity and diversity within the genus Gardnerella, highlighting the main features that disting

20 s ratio [AOR], 1.35 [95% CI, 1.05-1.91]) and Gardnerella immediately posttreatment (AOR, 1.23 [95% CI

21 to recommended treatment and persistence of Gardnerella immediately posttreatment may contribute to

22 alis and descriptions of three new species - Gardnerella leopoldii, Gardnerella piotii, and Gardnerel

23 Relative abundance of a novel Gardnerella metagenomic subspecies > 50% predicted sPTB

24 A novel Gardnerella metagenomic subspecies more abundant in wome

25 ococcus; 2, Prevotella; 3, Lactobacillus and Gardnerella); no species clusters were identified.

26 vaginal wash samples was associated with >30 Gardnerella or Prevotella morphotypes per high-power fie

27 subjects with CST 4 accompanied by elevated Gardnerella or Ureaplasma abundances.

28 Lactobacillus and Gardnerella organisms in urine specimens were excluded b

29 f three new species - Gardnerella leopoldii, Gardnerella piotii, and Gardnerella swidsinskii - have b

30 e due to BV biofilms protecting BV bacteria (Gardnerella, Prevotella, and other genera).

31 Instead, Bifidobacterium, Gardnerella, Prevotella, Pseudomonas, or Streptococcus p

32 Some of these bacteria, including Gardnerella, produce glycosyl hydrolase enzymes.

33 VK2 vaginal epithelial cells to recombinant Gardnerella sialidase led to desialylation of glycans an

34 ells from BV-negative women with recombinant Gardnerella sialidases generated BV-like glycan phenotyp

35 rococcus christenssii) on the spent media of Gardnerella species and perform metabolomics to identify

36 lts suggest that BV reflects a state of high Gardnerella species diversity.

37 Interestingly, we find that Gardnerella species drove this higher genetic diversity,

38 No Gardnerella species group was a specific marker for BV.

39 Concentrations and prevalence of each Gardnerella species group were significantly higher in p

40 1% of BV-positive participants had 3 or more Gardnerella species groups detected compared to 32.0% of

41 We hypothesized that particular Gardnerella species may be more associated with BV.

42 healthy women are colonized by nonpathogenic Gardnerella species, whereas virulent strains are involv

43 cillus and high prevalence of Prevotella and Gardnerella species.

44 vaginal and extra-vaginal colonization with Gardnerella spp, Megasphaera spp, Sneathia spp, BVAB-2,

45 We further present evidence that Gardnerella spp. underwent more frequent recombination a

46 complications (Aerococcus christensenii and Gardnerella spp.) among other facultative anaerobes and

47 vaginal and extra-vaginal colonization with Gardnerella spp., Megasphaera spp., Sneathia spp., BVAB-

48 rdnerella leopoldii, Gardnerella piotii, and Gardnerella swidsinskii - have been proposed.

49 12.2%), Lactobacillus iners (CST-LI, 43.6%), Gardnerella vaginalis (CST-GV, 26.6%), or polymicrobial

50 Trichomonas vaginalis, Candida species, and Gardnerella vaginalis (one of the causative agents of ba

51 eptotrichia/Sneathia species (P=0.0002), and Gardnerella vaginalis (P<0.0001).

52 Mycoplasma hominis and Gardnerella vaginalis also stimulated TNF- alpha secreti

53 d Bacterial vaginosis, enrichment of vaginal Gardnerella vaginalis and Lactobacillus iners was associ

54 iodemographics and BV, enrichment of vaginal Gardnerella vaginalis and Lactobacillus iners was associ

55 To understand complex interactions between Gardnerella vaginalis and Lactobacillus involved in effi

56 rapies resulted in decreased colonization by Gardnerella vaginalis and Mycoplasma hominis, only metro

57 diversity CSTs and specific bacterial phyla (Gardnerella vaginalis and Prevotella bivia) were strongl

58 appearance of lactobacilli and overgrowth of Gardnerella vaginalis and resident anaerobic vaginal bac

59 Both the proportion of women with Gardnerella vaginalis and the concentration of G. vagina

60 uding Megasphaera, Prevotella timonensis and Gardnerella vaginalis are associated with CIN2 persisten

61 Studies have implicated Gardnerella vaginalis as an important etiological agent

62 ; P = .009) bacterial communities containing Gardnerella vaginalis associated with vaginal drying, wh

63 Previous studies have shown that Gardnerella vaginalis can utilize iron-loaded human lact

64 When the two bacteria were combined, Gardnerella vaginalis did not interfere with the immunos

65 At baseline, Lactobacillus iners or Gardnerella vaginalis dominant vaginal communities were

66 ners was not, and that a subspecies clade of Gardnerella vaginalis explained the genus association wi

67 he 16S ribosomal RNA gene with clustering of Gardnerella vaginalis genomic clades.

68 Our data suggests that Gardnerella vaginalis had the highest virulence potentia

69 Gardnerella vaginalis has been considered a pivotal play

70 Gardnerella vaginalis has been implicated in BV but is a

71 ith suboptimal health including L. iners and Gardnerella vaginalis interact with both pro- and anti-i

72 Gardnerella vaginalis is a bacterial species associated

73 Gardnerella vaginalis is a predominant bacterial species

74 Gardnerella vaginalis is abundant in bacterial vaginosis

75 Gardnerella vaginalis is associated with a spectrum of c

76 Gardnerella vaginalis is detected in women with and with

77 man immunodeficiency virus (HIV) type 1, and Gardnerella vaginalis is frequently isolated from the ge

78 odeficiency virus (HIV) type 1 expression by Gardnerella vaginalis is one possible cause for an incre

79 Gardnerella vaginalis is the most common species found i

80 Of the 20 biotype 1 Gardnerella vaginalis isolates analyzed, 10 from patient

81 nae) load of 108 copies/mL or greater and/or Gardnerella vaginalis load of 109 copies/mL or greater,

82 al components of the score (ie, detection of Gardnerella vaginalis or Bacteroides spp and non-detecti

83 files were dominated by Lactobacillus iners, Gardnerella vaginalis or were highly diverse profiles.

84 by Lactobacillus (59.2%) and the other where Gardnerella vaginalis predominated with other anaerobic

85 Gardnerella vaginalis predominates in bacterial vaginosi

86 bacteria and overgrowth of anaerobes such as Gardnerella vaginalis spp.

87 Six Gardnerella vaginalis strains were examined for the abil

88 demonstrate that the BV-associated bacterium Gardnerella vaginalis uses sialidase to break down and d

89 al activities of L. crispatus, L. iners, and Gardnerella vaginalis vary with the taxonomic compositio

90 lactobacilli in the presence and absence of Gardnerella vaginalis was measured using Luminex.

91 Six strains of Prevotella bivia and 4 of Gardnerella vaginalis were examined for nutrient substra

92 ial activity against the primary BV pathogen Gardnerella vaginalis with a minimum inhibitory concentr

93 Gardnerella vaginalis, a facultative anaerobe, was clear

94 t F. nucleatum supported robust outgrowth of Gardnerella vaginalis, a major sialidase producer and on

95 trichia/Sneathia species, Atopobium species, Gardnerella vaginalis, and a Megasphaera-like bacterium,

96 ella pallens, Parvimonas micra, Megasphaera, Gardnerella vaginalis, and Atopobium vaginae and decreas

97 l Vaginosis-Associated Bacterium 2 (BVAB-2), Gardnerella vaginalis, and Megasphaera-1--and a single o

98 ween bacteria considered suboptimal, such as Gardnerella vaginalis, and metabolites enriched in term

99 Lactobacilli, Gardnerella vaginalis, and Mycoplasma hominis in cervico

100 HIV RNA, lactobacilli, Gardnerella vaginalis, and Mycoplasma hominis in cervico

101 athogenic bacteria: Lactobacillus crispatus, Gardnerella vaginalis, and Neisseria gonorrhoeae All vag

102 e to the vaginal pathogens Candida albicans, Gardnerella vaginalis, and Neisseria gonorrhoeae, as wel

103 n the two sampling devices for Candida spp., Gardnerella vaginalis, and Trichomonas vaginalis.

104 of lactobacilli and higher concentrations of Gardnerella vaginalis, Atopobium vaginae, and Prevotella

105 Lactobacillus iners, Gardnerella vaginalis, Atopobium vaginae, Megasphaera I,

106 but a typical bacterial vaginosis pathogen, Gardnerella vaginalis, had no effect.

107 mid suspension quantified Atopobium vaginae, Gardnerella vaginalis, lactobacilli, Mycoplasma hominis,

108 assays targeting 16S ribosomal RNA genes of Gardnerella vaginalis, Lactobacillus crispatus, BVAB1, B

109 cular mechanisms of pathogenicity factors of Gardnerella vaginalis, Mycoplasma genitalium, Mycoplasma

110 f hydrogen peroxide-producing lactobacillus, Gardnerella vaginalis, Mycoplasma hominis, anaerobic gra

111 rial vaginosis (BV), primarily attributed to Gardnerella vaginalis, poses significant challenges due

112 or of BV than detection of bacteria, such as Gardnerella vaginalis, previously linked to BV, highligh

113 Three BV-associated bacteria, Gardnerella vaginalis, Prevotella bivia, and Peptostrept

114 binding to the key vaginal bacteria species Gardnerella vaginalis, Prevotella bivia, Lactobacillus i

115 es (dominated by Lactobacillus crispatus and Gardnerella vaginalis, respectively), significant differ

116 ytolysins from Streptococcus intermedius and Gardnerella vaginalis, respectively.

117 actobacillus crispatus, Lactobacillus iners, Gardnerella vaginalis, S. agalactiae and F. nucleatum to

118 Gardnerella vaginalis, the bacterial species most freque

119 The BD Affirm assay includes a DNA probe for Gardnerella vaginalis, the Hologic transcription-mediate

120 V is initiated by the sexual transmission of Gardnerella vaginalis, which has the appropriate virulen

121 ms associated with vaginal health or disease:Gardnerella vaginalis,Atopobium vaginae, BV-associated b

122 focused solely on the BV-associated organism Gardnerella vaginalis.

123 obiota, consistently colonized by strains of Gardnerella vaginalis.

124 teria associated with bacterial vaginosis is Gardnerella vaginalis.

125 at BV is initiated by sexual transmission of Gardnerella vaginalis.

126 d, five suppressed inflammatory responses to Gardnerella vaginalis.

127 curtisii, Peptostreptococcus anaerobius, and Gardnerella vaginalis.

128 rial vaginosis-associated bacteria including Gardnerella vaginalis.

129 ysin produced by the BV-associated bacterium Gardnerella, verifying that it liberates contents of cer

130 lative abundance of genus Sneathia and genus Gardnerella was significantly increased in vulvovaginal

131 ommunities dominated by anaerobes other than Gardnerella were at over 4-fold higher risk of acquiring

132 rns of cooccurrence between L. crispatus and Gardnerella were highly exclusive, while Gardnerella and