ライフサイエンスコーパス: Golgi stack

1 ins to earlier or later locations within the Golgi stack.

2 e Toxoplasma gondii, which has only a single Golgi stack.

3 ies that it is used for transport across the Golgi stack.

4 gi intermediates and not to membranes of the Golgi stack.

5 pre-Golgi VTCs from peripheral sites to the Golgi stack.

6 embrane flow and cargo transport through the Golgi stack.

7 chimeric proteins were polarized within the Golgi stack.

8 uctures in recycling of Golgi enzymes in the Golgi stack.

9 nd have distinctive distributions within the Golgi stack.

10 atitis virus glycoprotein from the ER to the Golgi stack.

11 proteins and lipids as they move through the Golgi stack.

12 proteins localized to specific parts of the Golgi stack.

13 contributing to structural integrity of the Golgi stack.

14 ns may modulate the flow patterns within the Golgi stack.

15 from endoplasmic reticulum exit sites to the Golgi stack.

16 ng also abrogate Rab33B association with the Golgi stacks.

17 the lateral edges of, and often connecting, Golgi stacks.

18 eak leads to a precocious formation of large Golgi stacks.

19 h the elongation itself and the formation of Golgi stacks.

20 dicated that the fusion proteins located all Golgi stacks.

21 to function in the postmitotic reassembly of Golgi stacks.

22 ganization of the pericentriolarly localized Golgi stacks.

23 al proteins, RNA, and apolipoproteins in the Golgi stacks.

24 axin 5 and membrin penetrate deeply into the Golgi stacks.

25 ialyl transferase to GFP was targeted to the Golgi stacks.

26 he absence of IQ is sufficient to vesiculate Golgi stacks.

27 abolite, causes complete vesiculation of the Golgi stacks.

28 on of the chimeric proteins occurred between Golgi stacks.

29 endoplasmic reticulum and fails to enter the Golgi stacks.

30 o transport vesicles at the terminal rims of Golgi stacks.

31 r sucrose transporter SUC4 is blocked in cis-Golgi stacks.

32 e reassembly of the Golgi fragments into new Golgi stacks.

33 eads to reassembly of the membranes into new Golgi stacks.

34 the daughter cells and reassembled into new Golgi stacks.

35 s and large skein-like structures entangling Golgi stacks.

36 ccompanied by a significant proliferation of Golgi stacks.

37 r cells, where they are reassembled into new Golgi stacks.

38 ding plastids, mitochondria, peroxisomes and Golgi stacks.

39 sec16 mutant is accompanied by disruption of Golgi stacks.

40 omologue was isolated and shown to target to Golgi stacks.

41 f ST6GalNAc-I, which is found throughout the Golgi stacks.

42 the proportion of GRIP-GFP fusion protein on Golgi stacks.

43 in-13A and GhKinesin-13A localized to entire Golgi stacks.

44 are needed in combination for GRASP-mediated Golgi stacking.

45 ns and provide insight into the mechanism of Golgi stacking.

46 th antibodies to GRASP65 fail to form proper Golgi stacks after cell division.

47 During metaphase, approximately 20% of all Golgi stacks aggregate in the immediate vicinity of the

48 5 and GRASP55, two proteins localized to the Golgi stack and early secretory pathway, mediate process

49 Golgi stack and peroxisome movements were also significa

50 e more complete protein glycosylation in the Golgi stack and proper sorting at the trans-Golgi networ

51 izing agents promote the alkalization of the Golgi stack and thetrans-Golgi network.

52 al micrometers) emanate from elements of the Golgi stack and trans Golgi network (TGN).

53 rganelles of the host cell (lysosomes, trans-Golgi stack and trans-Golgi network, and endoplasmic ret

54 X9 is present in a specific subdomain of the Golgi stack and was most abundant in the ring of the inn

55 ein 65 (GRASP65) has been implicated in both Golgi stacking and ribbon linking by forming trans-oligo

56 65 kDa (GRASP65) has been implicated in both Golgi stacking and ribbon linking by forming trans-oligo

57 phy and immunolabeling techniques to examine Golgi stacks and associated vesicles in the cells of the

58 Ultrastructural analysis revealed enlarged Golgi stacks and increased transitional vesicles in vent

59 Reclustering of nocodazole-dispersed Golgi stacks and microtubule/dynein-dependent ER-to-Golg

60 e find the Golgi protein in the newly formed Golgi stacks and not in the ER.

61 t TGN cisternae apparently peel off from the Golgi stacks and persist for some time in the cytosol, a

62 Detergent-extracted Golgi stacks and TGN-containing membranes are closely as

63 es that these spots correspond to individual Golgi stacks and that the fusion protein is largely conf

64 lgi with lobe A preferential localization on Golgi stacks and the presence of lobe B on vesicle-like

65 e implicated in the architecture of both the Golgi stacks and the tER sites.

66 lgi marker indicates that RabA2 localizes in Golgi stacks and the trans-Golgi network.

67 ve cell homogenate) and by appearance of the Golgi stacks and vesicles on electron microscopy.

68 secretory cargoes could be restricted at the Golgi stack, and the entry of the trans-Golgi network in

69 he adjacent Golgi networks compared with the Golgi stack, and this enrichment varies with cell type.

70 erent targets in unperturbed and dissociated Golgi stacks, and investigating and quantifying inter-or

71 yeasts: Pichia pastoris, which has coherent Golgi stacks, and Saccharomyces cerevisiae, which has a

72 detected in WIGs, the endoplasmic reticulum, Golgi stacks, and the trans-Golgi network in the Mn1 BET

73 maintenance of the interconnected ribbons of Golgi stacks, and tubule formation from endosomes.

74 ructural and enzymatic components of the new Golgi stack are laid down first, followed by those neede

75 It is thought that residents of the Golgi stack are localized by a retention mechanism that

76 sent evidence for a third model in which the Golgi stacks are a continuous structure and proteins rap

77 In P. pastoris, Golgi stacks are adjacent to discrete tER sites that con

78 We find that tER sites and the adjacent Golgi stacks are enclosed in a ribosome-excluding "matri

79 The pericentriolar Golgi stacks are fragmented and found dispersed in mitot

80 nzymes reside; in mammalian cells, dozens of Golgi stacks are often laterally linked into a ribbon-li

81 Golgi stacks are often located near sites of "transition

82 However, the scattered Golgi stacks are polarized and transport cargo.

83 nesis is consistent with the hypothesis that Golgi stacks are repositioned to ensure equal partitioni

84 velopment, in fine-tuning the positioning of Golgi stacks, as well as their involvement in cellulose

85 tory proteins, the overriding principle of a Golgi stack assembly is likely to be quite simple.

86 The ecdysone-triggered biogenesis of Golgi stacks at the onset of imaginal disc elongation of

87 Regeneration of Golgi stacks at these peripheral sites would re-establis

88 his event increases before the dispersion of Golgi stacks, at the onset of cell migration.

89 nstrate that both GRASPs are dispensable for Golgi stacking but are involved in maintaining the integ

90 green fluorescent protein (GFP), locates to Golgi stacks but does not exactly co-locate with the Gol

91 t each MT population is capable of gathering Golgi stacks but not of establishing Golgi complex integ

92 cking the golgin TRIP11/GMAP-210 have normal Golgi stacks, but show developmental problems related to

93 GRASP55, Golgi stack length is shortened but Golgi stacking, compartmentalization, and transport seem

94 r wide area (FRAP-W) experiments, peripheral Golgi stacks continuously exchanged resident proteins wi

95 les and Kinesin-13A, and the organization of Golgi stacks could play a regulatory role in trichome mo

96 inesis, the daughter cells have very similar Golgi stack densities.

97 We have followed the redistribution of Golgi stacks during mitosis and cytokinesis in living to

98 The redistribution of Golgi stacks during mitosis and cytokinesis is consisten

99 o be phosphorylated, allows the cell to keep Golgi stacks during mitosis and has no effect on the equ

100 n drives HPV trafficking through the TGN and Golgi stacks during virus entry.

101 Golgi in HeLa cells, we stably expressed the Golgi stack enzyme N-acetylgalactosaminyltransferase-2 (

102 ate glycosylation, with the focus on how the Golgi stacking factors GRASP55 and GRASP65 generate the

103 posited within the cis-most cisternae of the Golgi stack following a 15 degrees C block.

104 Depletion of GRASP proteins disrupts Golgi stack formation and impairs critical functions of

105 In vitro studies have suggested that Golgi stack formation involves two homologous peripheral

106 shown that GRASP depletion in cells disrupts Golgi stack formation.

107 ules and Golgi stacks indicated an increased Golgi stack frequency at the preprophase band site.

108 pic event is accompanied by the formation of Golgi stacks from small Golgi larval clusters of vesicle

109 In mammalian cells, individual Golgi stacks fuse laterally to form the characteristic p

110 of collective migration of epithelial cells, Golgi stacks get dispersed to create an unpolarized tran

111 cretory pathway, mediate processes including Golgi stacking, Golgi ribbon linking and unconventional

112 insect cells that naturally harbor dispersed Golgi stacks have limited capacity to transport artifici

113 nonphosphorylatable GRASP55 mutants enhances Golgi stacking in interphase cells and inhibits Golgi di

114 event the formation of membrane tubules from Golgi stacks in an in vitro reconstitution system.

115 Golgi stacks in border cells and peripheral cells, precu

116 confirmed using brefeldin A to collapse the Golgi stacks in both HEK 293 and COS-1 cells.

117 kinase 1 (MEK1) fragments the pericentriolar Golgi stacks in mammalian cells.

118 Golgi stacks in the border cells have hypertrophied marg

119 ms in the nucleated karyoplasts, whereas the Golgi stacks in the cytoplasts are scattered.

120 The Golgi stacks in these GRASP-deficient HeLa cells were no

121 We show now that Golgi stacks in vitro bind dynein supplied from cytosol

122 Golgi stacks, in permeabilized interphase normal rat kid

123 ving BY-2 cells labeled for microtubules and Golgi stacks indicated an increased Golgi stack frequenc

124 in hand with the compartmentalization of the Golgi stack into cis-, medial-, and trans-cisternae, whi

125 ble knockout of GRASP proteins disperses the Golgi stack into single cisternae and tubulovesicular st

126 zed homotypic membrane tethers that organize Golgi stacks into a long, contiguous ribbon-like structu

127 with brefeldin A or if GRASP65, which links Golgi stacks into a ribbon network, was depleted.

128 emains puzzling how GRASP65 physically links Golgi stacks into a ribbon.

129 xpressing NSF(E329Q) promotes disassembly of Golgi stacks into dispersed vesicular structures.

130 Mitotic conversion of Golgi stacks into mitotic clusters had surprisingly litt

131 A single Golgi stack is duplicated and partitioned into two daugh

132 ay ensure that the pool of UDP-GlcNAc in the Golgi stack is not depleted, thereby maintaining proper

133 ence of GRASP65, the number of cisternae per Golgi stack is reduced without affecting the overall org

134 These results indicate that although Golgi stacking is a highly complicated process involving

135 motor myosin-I and dynein, whereas isolated Golgi stacks lack dynein but contain myosin-I.

136 In the absence of GRASP55, Golgi stack length is shortened but Golgi stacking, comp

137 The sites of preferential Golgi stack localization are specific for this organelle

138 that certain tether-SNARE interaction within Golgi stack may play a role in inhibiting intercisternal

139 How does the Golgi stack mediate transport of cargo from the endoplas

140 s, illustrating how the most ancient form of Golgi stacking might have occurred using only weak ciste

141 We further report here that movement of Golgi stacks, mitochondria, and peroxisomes in the leaf

142 s revealed independent movement patterns for Golgi stacks, mitochondria, and peroxisomes, indicating

143 osin XI-K has a major role in trafficking of Golgi stacks, mitochondria, and peroxisomes, whereas myo

144 The Golgi stack, monitored using sialyltransferase, galactos

145 ed to important morphological changes in the Golgi stack morphology and the transitional ER (tER) org

146 ng ERES, and that an ERES and its associated Golgi stack move as a single secretory unit.

147 dding yeast Pichia pastoris contains ordered Golgi stacks next to discrete transitional endoplasmic r

148 olling the transport of proteins through the Golgi stack of mammalian and plant cells is the subject

149 Likewise, reformation of Golgi stacks on removal of BFA was not dependent on eith

150 e elements could be positively identified as Golgi stacks or cisternae.

151 of these myosins also reduced trafficking of Golgi stacks, peroxisomes, and mitochondria in root hair

152 ndant and additive roles in the transport of Golgi stacks, peroxisomes, and mitochondria.

153 h the cisternal margins and the TGN, whereas Golgi stack proliferation was unaffected.

154 nases, cdc2 and plk, which phosphorylate the Golgi stacking protein GRASP65 and thus disrupt the olig

155 Grasp55 is a ubiquitous Golgi stacking protein involved in autophagy, protein tr

156 ssembly and stacking protein 55 kDa (G55), a Golgi stacking protein that maintains Golgi organelle in

157 d was also observed for ERGIC-53 but not for Golgi stack proteins.

158 olgi cisternae requires dephosphorylation of Golgi stacking proteins by the protein phosphatase PP2A.

159 gree, functionally substitute for GRASP-type Golgi stacking proteins to sustain normal Golgi morpholo

160 It has been demonstrated that two Golgi stacking proteins, GRASP55 and GRASP65, self-inter

161 kDa (GRASP55) were originally identified as Golgi stacking proteins; however, subsequent GRASP knock

162 les to the nucleus while G3 continues to the Golgi stacks, providing passage for the entire core prot

163 During telophase and cytokinesis, many Golgi stacks redistribute around the phragmoplast where

164 cks stops, about one-third of the peripheral Golgi stacks redistributes to the perinuclear cytoplasm,

165 o untreated cells, suggesting that polarized Golgi stacks reform rapidly at scattered sites.

166 STB increases the rate of MT-dependent Golgi stack repositioning after nocodazole treatment.

167 he endoplasmic reticulum (ER) and within the Golgi stack, sorting transmembrane proteins bearing C-te

168 cell division, when the general streaming of Golgi stacks stops, about one-third of the peripheral Go

169 educed the number of cisternal membranes per Golgi stack, suggesting a loss of trans-Golgi cisternae.

170 e for a countercurrent fusion pattern in the Golgi stack, the gradients involved would be strongly sh

171 m (ER) but does not disrupt the formation of Golgi stacks, the distribution of beta-COP, or the trans

172 hase, there are many scattered, disconnected Golgi stacks throughout the cytoplasm, which appear as 1

173 on that it is broadly distributed across the Golgi stack to serve the many sialyltransferases involve

174 LTA(1) localization from cytosol to binding Golgi stacks to condensation of Golgi membranes was foun

175 a method for inducing plant cells and their Golgi stacks to differentiate in a synchronous manner ha

176 luorescent protein fusion redistributes from Golgi stacks to the perinuclear region, where poliovirus

177 In Arabidopsis AtPAT10 is localized in the Golgi stack, trans-Golgi network/early endosome and tono

178 ls consists of a large number of independent Golgi stack/trans-Golgi network/Golgi matrix units that

179 uclear area and a partial disassembly of the Golgi stack under electron microscope within 3-5 h, sugg

180 that this ER-rich peripheral region excludes Golgi stacks, vacuoles, and amyloplasts but not mitochon

181 quent movement of ER-derived carriers to the Golgi stack was blocked by a trans-dominant ARF1 mutant

182 ntermediates with cisternal membranes of the Golgi stack was not observed under these conditions.

183 Aggregation/clustering of Golgi stacks was often observed in the kinesin-13a mutan

184 ivity of Plk3; Plk3-induced fragmentation of Golgi stacks was significantly reduced after treatment w

185 res and macromolecular compositions of these Golgi stacks, we examined high-pressure frozen/freeze-su

186 immunofluorescence microscopy, we show that Golgi stacks were converted into clusters of vesicles an

187 In UL133-UL138(NULL) virus-infected ECs, Golgi stacks were disrupted, and the viral assembly comp

188 wild-type and kinesin-13a mutant cells, the Golgi stacks were frequently associated with microtubule

189 n effect on the tER organization, though the Golgi stacks were greatly vesiculated in the cells deple

190 Furthermore, although Golgi stacks were randomly distributed, the organization

191 istributed to peripheral ER exit sites where Golgi stacks were regenerated.

192 Notably, in the absence of functional GNL1, Golgi stacks were rendered sensitive to the selective AR

193 The Golgi stacks were shown to move rapidly and extensively

194 by siRNA reduces the number of cisternae per Golgi stack, whereas simultaneous knockdown of both GRAS

195 e tER sites and therefore generates coherent Golgi stacks, whereas S. cerevisiae has a delocalized tE

196 RAB11A, SNAP23, and CDC42 caused the loss of Golgi stacks with reorganization into structures that re

197 rom oligosaccharide-modifying enzymes in the Golgi stack without affecting their ability to form a ri