ライフサイエンスコーパス: Golgin

1 hagy, demonstrating an unexpected role for a golgin.

2 d on a family of coiled-coil proteins called golgins.

3 sophila orthologs of these three intra-Golgi golgins.

4 d a C-terminal region (CTR) interacting with golgins.

5 olecular weight coiled-coil proteins, termed golgins.

6 , and various cargoes specific to individual golgins.

7 been classified as a family of proteins, the golgins [1].

8 These include GM130, golgin 160, and the Golgi vesicle tethering protein p115

9 dominantly prevented cleavage of endogenous golgin-160 after ligation of death receptors or inductio

10 Here, we identify golgin-160 and GMAP210 as proteins required for centripe

11 ese findings not only reveal novel roles for golgin-160 and GMAP210 in conferring membrane motility b

12 Full-length golgin-160 and golgin-160B may link distinct subsets of

13 Golgin-160 and PIST colocalize to Golgi membranes and in

14 rminus binds a GSV anchoring site comprising Golgin-160 and possibly other proteins.

15 some pro-apoptotic stimuli; thus cleavage of golgin-160 appears to play a role in apoptotic signaling

16 Using the N-terminal head domain of golgin-160 as bait in a yeast two-hybrid screen, the pos

17 lized to the Golgi complex, where it cleaves golgin-160 at a unique site not susceptible to cleavage

18 Golgin-160 belongs to the golgin family of Golgi-localiz

19 P60 interacts preferentially with one of the golgin-160 caspase cleavage fragments (residues 140-311)

20 s rescued by expression of a siRNA-resistant golgin-160 cDNA.

21 ptosis in cells expressing caspase-resistant golgin-160 could not be bypassed by expression of potent

22 The caspase-resistant golgin-160 dominantly prevented cleavage of endogenous g

23 itro and disrupted the ability to retain the golgin-160 fragment at the Golgi in cells.

24 This strong interaction prevented the golgin-160 fragment from accumulating in the nucleus whe

25 raction increases the Golgi retention of the golgin-160 fragment in cells overexpressing GCP60.

26 nteraction with one of the caspase-generated golgin-160 fragments (residues 140-311).

27 ear translocation and potential functions of golgin-160 fragments.

28 Golgin-160 has a large coiled-coil C-terminal domain and

29 This is the first time that Golgin-160 has been shown to be required for ECM secreti

30 ybrid assay to screen for interactors of the golgin-160 head and identified GCP60 (Golgi complex-asso

31 event regulated not only by cleavage of the golgin-160 head but also by the oxidation state of GCP60

32 resident protein, interacts weakly with the golgin-160 head domain but has a strong interaction with

33 Caspase cleavage of the golgin-160 head domain generates different fragments tha

34 Because the golgin-160 head is cleaved by caspases during apoptosis,

35 Expression of a non-cleavable form of golgin-160 impairs apoptosis induced by some pro-apoptot

36 asma membrane proteins, supporting a role of golgin-160 in vesicular trafficking.

37 Golgin-160 is a coiled-coil protein on the cytoplasmic f

38 Golgin-160 is a member of the golgin family of Golgi-loc

39 The peripheral Golgi protein golgin-160 is induced during 3T3L1 adipogenesis and is p

40 The COOH-terminal two-thirds of golgin-160 is predicted to form a coiled-coil, with an N

41 Golgin-160 is ubiquitously expressed in vertebrates.

42 distinct impacts on Golgi organization, with Golgin-160 knockout causing Golgi fragmentation and vesi

43 Therefore, our data suggest that golgin-160 may participate in PIST-dependent trafficking

44 ents identified a leucine-rich repeat within golgin-160 necessary for interaction with PIST.

45 Together, these data demonstrate that golgin-160 plays an important role in directing insulin-

46 d and integrated at Golgi membranes and that golgin-160 plays an important role in transduction of th

47 Golgin-160 possesses an N-terminal non-coiled-coil "head

48 nterfering RNA (siRNA)-mediated reduction in golgin-160 protein resulted in an increase accumulation

49 information is present in the same region of golgin-160 suggests that this protein may have more than

50 ntact TUG links GLUT4 to PIST and also binds Golgin-160 through its C-terminal region.

51 ly expressing wild-type or caspase-resistant golgin-160 to several proapoptotic stimuli.

52 itive; in its reduced form, interaction with golgin-160 was diminished or abolished, whereas oxidatio

53 ing to the plasma membrane in the absence of golgin-160 was independent of TGN/Golgi sorting, because

54 lls expressing a caspase-resistant mutant of golgin-160 were strikingly resistant to apoptosis induce

55 Golgin-160, a ubiquitous protein in vertebrates, localiz

56 identify the Golgi targeting information in golgin-160, full-length and deletion constructs tagged w

57 lso discovered a widely expressed isoform of golgin-160, golgin-160B, which lacks the exon encoding t

58 f two Golgi organizing proteins, GMAP210 and Golgin-160, in ECM secretion.

59 n of potential caspase cleavage fragments of golgin-160, or by drug-induced disassembly of the Golgi

60 ndings suggest that nuclear translocation of golgin-160-(140-311) is a highly coordinated event regul

61 rosporine, suggesting that nuclear-localized golgin-160-(140-311) might promote cell survival.

62 Here we studied the interaction of golgin-160-(140-311) with GCP60 and identified a single

63 The TUG C-terminal peptide bound the Golgin-160-associated protein, ACBD3 (acyl-CoA-binding d

64 TC10 (PIST) was identified to interact with golgin-160.

65 d-coil domain, which interacts directly with golgin-160.

66 x in the absence of assembly with endogenous golgin-160.

67 dies against Golgin-95/GM130, Golgin-97, and Golgin-160.

68 otein related to the mammalian Golgi protein golgin-160.

69 n reduced binding of TUG to ACBD3 but not to Golgin-160.

70 gin-230/245/256, golgin-97, GM130/golgin-95, golgin-160/MEA-2/GCP170, giantin/macrogolgin and a relat

71 Full-length golgin-160 and golgin-160B may link distinct subsets of proteins to eff

72 As predicted, golgin-160B was unable to bind PIST.

73 ed a widely expressed isoform of golgin-160, golgin-160B, which lacks the exon encoding the leucine r

74 lgi apparatus of two members of this family, golgin-230/245/256 and golgin-97, was investigated.

75 This family includes golgin-230/245/256, golgin-97, GM130/golgin-95, golgin-1

76 The Golgi-localized golgins golgin-97 and golgin-245 capture transport vesicles arriving from endo

77 Thus, TBC1D23 attached to golgin-97 and golgin-245 captures vesicles by a previously undescribed

78 RIP domain-containing proteins Golgin-97 and Golgin-245 from the trans-Golgi.

79 een ARL1 and two binding proteins, SCOCO and Golgin-245, are defined and characterized in more detail

80 sm acting on the transport of Golgin-97- vs. Golgin-245-dependent cargoes.

81 In contrast, PARP12 does not control the Golgin-245-dependent traffic of cargoes such as tumor ne

82 trixlike tail region that sometimes contains Golgin-245.

83 those tethered by GCC88 but not golgin-97 or golgin-245.

84 vesicle tethering proteins GM130, p115, and Golgin-45 from the Golgi and compromises ribbon linking.

85 These findings suggest that Golgin-45 serves as a linchpin for the maintenance of Go

86 al. report the discovery of a protein named Golgin-45 that is located on the surface of the middle (

87 of the Golgi ribbon together with GM130 and Golgin-45.

88 oteins and report here the identification of golgin-84 as a novel mitotic target.

89 Golgin-84 binds to active rab1 but not cis-Golgi matrix

90 t showed that the N-terminal 497 residues of golgin-84 contain a coiled-coil domain that when fused t

91 the volume of a normal Golgi apparatus upon golgin-84 depletion.

92 Cross-linking indicates that golgin-84 forms dimers, consistent with the prediction o

93 Golgin-84 is an integral membrane protein with a single

94 Data base searching also indicates golgin-84 is similar in structure and sequence to gianti

95 Microinjected golgin-84 or CASP also inhibited Golgi-enzyme transport

96 Our results suggest that golgin-84 plays a key role in the assembly and maintenan

97 Overexpression or depletion of golgin-84 results in fragmentation of the Golgi ribbon.

98 ng cryoelectron microscopy we could localize golgin-84 to the cis-Golgi network and found that it is

99 II, GOS-28, GS15, GPP130, CASP, giantin, and golgin-84) whose abundances were reduced in the mutant c

100 mammalian cells, three of the golgins, TMF, golgin-84, and GMAP-210, can capture intra-Golgi transpo

101 e best studied, and here we characterize the golgin-84-CASP tether.

102 approximately 84-kDa Golgi protein we termed golgin-84.

103 ncludes golgin-230/245/256, golgin-97, GM130/golgin-95, golgin-160/MEA-2/GCP170, giantin/macrogolgin

104 irmed by using polyclonal antibodies against Golgin-95/GM130, Golgin-97, and Golgin-160.

105 stored proper localization of syntaxin 6 and golgin 97 (key proteins in membrane trafficking at TGN)

106 e for a distinct class of vesicles shared by golgin-97 and GCC88, and various cargoes specific to ind

107 The golgin coiled-coil proteins golgin-97 and GCC88, shown previously to capture endosom

108 Thus, TBC1D23 attached to golgin-97 and golgin-245 captures vesicles by a previous

109 sociation of GRIP domain-containing proteins Golgin-97 and Golgin-245 from the trans-Golgi.

110 hermore, by proteomic analysis we identified Golgin-97 as a FIP1/RCP-binding protein.

111 PARP12 targets an acidic cluster in the Golgin-97 coiled-coil domain essential for function.

112 Binding of FIP1/RCP to Golgin-97 does not affect Golgin-97 recruitment to the T

113 discuss the potential role and functions of golgin-97 in poxvirus replication and propose two workin

114 Inside the virion, golgin-97 is associated with the insoluble core protein

115 Thus, we propose that FIP1/RCP binding to Golgin-97 is required for tethering and fusion of recycl

116 asma membrane is differentially regulated by Golgin-97 mono-ADP-ribosylation by PARP12.

117 PARP12 enzymatic activity, and consequently Golgin-97 mono-ADP-ribosylation, depends on the activati

118 estricted to those tethered by GCC88 but not golgin-97 or golgin-245.

119 ing of FIP1/RCP to Golgin-97 does not affect Golgin-97 recruitment to the TGN, but appears to regulat

120 lular localization of the trans-Golgi marker Golgin-97 suggested differences in the organization of t

121 We find that Golgin-97, a TGN protein required for the formation and

122 RCP-binding domain maps to the C-terminus of Golgin-97, adjacent to its GRIP domain.

123 lyclonal antibodies against Golgin-95/GM130, Golgin-97, and Golgin-160.

124 gi complex with specific markers, anti-human Golgin-97, anti-KDEL receptor, and BODIPY-TR ceramide, s

125 51 peripheral membrane proteins re-routed by golgin-97, evidence for a distinct class of vesicles sha

126 This family includes golgin-230/245/256, golgin-97, GM130/golgin-95, golgin-160/MEA-2/GCP170, gia

127 esiding in the trans-Golgi network membrane, golgin-97, is transported to the sites of virus replicat

128 mbers of this family, golgin-230/245/256 and golgin-97, was investigated.

129 ulatory mechanism acting on the transport of Golgin-97- vs. Golgin-245-dependent cargoes.

130 ing only partial overlap with the TGN marker golgin-97.

131 g by serving as a bridge between Golgi-bound golgin-97/245 and the WASH/FAM21 complex on endosomal ve

132 the zebrafish model, we show that impacting golgin-97/245-binding, but not the putative catalytic si

133 forms part of the platform to interact with golgin-97/245.

134 sferase 5 (ZDHHC5) and an accessory protein, golgin A7 (GOLGA7, also known as GCP16).

135 These findings demonstrate that golgins act as tethers in vivo, and hence the specificit

136 Overall, our study reveals a role for a TGN golgin and ITSN-1 in linking to the actin cytoskeleton a

137 e the distance between the distal end of the golgin and the target membrane thereby promoting tighter

138 whether its mechanism of action requires its golgin and/or SNARE interactions.

139 TPases, ARL1 and ARL5, which in turn recruit golgins and GARP, respectively, to the TGN.

140 e propose that long-range tethers, including Golgins and multisubunit tethering complexes, hand off v

141 f COPI vesicles by first using long tethers (Golgins) and then short tethers (SNAREs).

142 mino-terminal domain of TBC1D23 binds to the golgins, and the carboxyl-terminal domain of TBC1D23 cap

143 We therefore propose that golgins are collectively required to create the correct

144 Golgins are extended coiled-coil proteins believed to pa

145 Golgins are Golgi-localized proteins present in all mole

146 Loss-of-function mutants show that the golgins are individually dispensable, although the loss

147 However, the individual golgins are not required for cell viability, and mouse k

148 Golgins are thought to form extended homodimers that are

149 malian cells more emphasis has been given to golgins as a potentially stable assembly framework.

150 Moreover, although golgins bind to Rab GTPases, the functional significance

151 115 lacking its phosphorylation site and the golgin-binding domains also restored the Golgi apparatus

152 The golgin coiled-coil proteins golgin-97 and GCC88, shown p

153 -tethering activities of 10 widely conserved golgin coiled-coil proteins.

154 e reported that the Saccharomyces cerevisiae golgin Coy1 contributes to intra-Golgi retrograde transp

155 ltransferases and glycosidases into nascent, golgin-enriched structures after drug washout.

156 hout in HeLa cells, we found that scattered, golgin-enriched, structures formed early and contained g

157 Coiled-coil proteins of the golgin family have been implicated in intra-Golgi transp

158 mall GTPases and coiled-coil proteins of the golgin family help to tether COPI vesicles to Golgi memb

159 x proteins--the best characterized being the golgin family of fibrous, coiled-coil proteins and the G

160 Golgin-160 is a member of the golgin family of Golgi-localized membrane proteins.

161 Golgin-160 belongs to the golgin family of Golgi-localized proteins, which have be

162 A subset of the golgin family of large coiled-coil proteins have a GRIP

163 We show here that GCC185, a member of the Golgin family of putative tethering proteins, is a Rab9

164 Extended coiled-coil proteins of the Golgin family play prominent roles in maintaining the st

165 ing factors: long coiled-coil tethers of the golgin family, and the heterotetrameric complex GARP.

166 We have found that these VCs use different golgins for docking: C2GnT-M-carrying VC (C2GnT-M-VC) ut

167 excludes a phylogenetically deeply conserved golgin from the nervous system, paralleling surprising s

168 he trans-Golgi network (TGN) membrane tether/golgin, GCC88, modulates the Golgi ribbon architecture.

169 Knockout of the golgin giantin leads to skeletal and craniofacial defect

170 cidic COOH-terminal domain of p115 links the Golgins, Giantin on COPI vesicles, to GM130 on Golgi mem

171 The golgin GM130 is thought to bind the C-terminal side of t

172 We show that the cis golgin GMAP-210 accounts for this property.

173 In this study, we show that depletion of the golgin GMAP-210 causes a loss of Golgi cisternae and acc

174 Hence, our results reveal a function for the golgin GMAP210 conveying specific vesicles to the immune

175 Here we show that the golgin GMAP210, known to capture vesicles and organize m

176 racterized genes by identifying ten putative golgins/Golgi-associated proteins amongst 8219 genes of

177 The Golgi-localized golgins golgin-97 and golgin-245 capture transport vesic

178 Apart from giantin and GM130, none of the golgins has yet been assigned a function in the Golgi ap

179 Golgins have been implicated in the maintenance of Golgi

180 oorly defined, and alternative functions for golgins have been proposed.

181 cleotide exchange factor Gea2 to recruit the golgin Imh1 to the Golgi.

182 d suppress Ypt6 dysfunction via its effector golgin, Imh1, but not via the lipid flippase Drs2.

183 1p, which was accompanied by accumulation of golgin Imh1p at late Golgi, but whether and how this fun

184 plex is specifically required for recruiting golgin Imh1p to the Golgi.

185 However, the biological function of this golgin in skin is unknown.

186 iched, structures formed early and contained golgins including giantin, ranging across the entire cis

187 Some peripheral membrane Golgins, including the yeast Imh1 protein, contain the r

188 nce microscopy were employed to identify the golgins involved in the Golgi docking of vesicular compl

189 captured by a particular golgin when another golgin is missing reveals that the vesicle content in on

190 urs in the absence of GRASP65/55 when either Golgin is overexpressed, as judged by quantitative elect

191 t the pathway involving ARL1 and GRIP domain golgins is conserved in plants.

192 Lrrk interacted with the golgin Lava lamp and inhibited the interaction between L

193 The gene, designated GLP (for Golgin linked to PML), is strongly expressed as a 6-kb t

194 direct vesicle arrival at the Golgi are the golgins, long coiled-coil proteins localized to specific

195 Golgins, long coiled-coil proteins that localize to part

196 These and other golgins may modulate the flow patterns within the Golgi

197 Whereas the golgins mediate long-distance capture of the carriers, G

198 However, the importance of golgin-mediated tethering remains poorly defined, and al

199 proteins 55 and 65 (GRASP55 and GRASP65) and Golgin of 45 kDa and Golgi matrix protein of 130 kDa.

200 with long coiled-coil domains, like lamins, golgins, or microtubule organization center components,

201 Golgin p230 and TGN46 are separated into distinct vesicl

202 In the absence of either golgin, peri-centrosomal positioning of the Golgi appara

203 ng mitosis, we found that the formation of a golgin-positive acceptor compartment in early telophase

204 aging experiments reveal that the linking of Golgins precedes SNAREpin assembly.

205 Here we further investigate the Golgin protein Coy1 and document its function in retrogr

206 via an interaction between their CTD and the Golgin protein GCC185.(6) This allows microtubule growth

207 us binding between ciliary protein IFT20 and golgin protein GMAP210 in these osteoblasts.

208 eal the regulatory mechanisms of ciliary and golgin proteins required for intramembranous ossificatio

209 The golgin, RAB6-interacting (GORAB) protein localizes to th

210 GLP is 50% homologous to other Golgin-related proteins including the vesicle docking pr

211 f a gene with strong homology to a family of Golgin-related proteins.

212 However, the deletion of multiple golgins results in defects in glycosylation and loss of

213 with deletions in other putative retrograde Golgins (sgm1Delta and rud3Delta) caused strong glycosyl

214 proteomics analysis revealed both shared and golgin-specific changes in the secretion of ECM proteins

215 ser predicted protein-coding genes, GOLGA8E (golgin subfamily a, 8E) and WHDC1L1 (WAS protein homolog

216 GOLGA8E belongs to the golgin subfamily of coiled-coil proteins associated with

217 Six out of 16 golgin subfamily proteins in the human genome have been

218 ifferent Rabs in addition to each end of the golgin "TATA element modulatory factor" (TMF).

219 ingly different from those bound by the p115-golgin tether in that they lacked members of the p24 fam

220 The p115-golgin tether is the best studied, and here we character

221 We had previously shown that the two Golgin tethers (GM130 and Golgin45) could, to a large de

222 ouble depletion of the two Syntaxin5-binding Golgin tethers also led to significant intercisternal fu

223 GORAB is a golgin that localizes predominantly at the Golgi apparat

224 surprising spatial specificities of another golgin that we describe here as well.

225 GRIP domain proteins are a class of golgins that have been described in yeast and animals.

226 r to obtain clues as to the functions of the golgins, the targeting to the Golgi apparatus of two mem

227 In mammalian cells, three of the golgins, TMF, golgin-84, and GMAP-210, can capture intra

228 rogression, implicating it as the only known golgin to regulate both mitosis and apoptosis.

229 s have shown, however, that mice lacking the golgin TRIP11/GMAP-210 have normal Golgi stacks, but sho

230 rgo present in the vesicles captured by each golgin varies between tissues.

231 Both golgins were required for fibrillar collagen organizatio

232 mining the vesicles captured by a particular golgin when another golgin is missing reveals that the v

233 n this study, we report that the GRIP domain golgins, whose C termini bind the Arf-like 1 G protein o

234 We find that subsets of golgins with distinct localizations on the Golgi surface