ライフサイエンスコーパス: H. hepaticus

1 H. hepaticus causes chronic active hepatitis, with progr

2 H. hepaticus had a strong affinity for molecular H(2) (a

3 H. hepaticus infection elicited both T cell-mediated and

4 H. hepaticus present in feces and cecal samples from H.

5 H. hepaticus was cultured from fetal viscera of 2 of 11

6 H. hepaticus was orally inoculated into 30 axenic, outbr

7 H. hepaticus, which forms a spreading film on selective

8 H. hepaticus, which persists in the lower bowels and liv

9 H. hepaticus-induced colitis is associated with elevated

10 H. hepaticus-infected BALB-RagMin mice developed moderat

11 H. hepaticus-infected mice seroconverted by 2 weeks and

12 H. hepaticus-infected parental strains including A/J and

13 H. hepaticus-infected Rag2-/-, but not sham-dosed Rag2-/

14 H. hepaticus-infected wild-type mice did not develop inf

15 ith H. hepaticus in tissue sections and to a H. hepaticus protein (70 kd) in Western blots.

16 with regulatory cells at 4 or 12 weeks after H. hepaticus infection had reduced severity of inflammat

17 d no serum immunoglobulin G response against H. hepaticus.

18 obulin G1 (IgG1) and IgG2c responses against H. hepaticus, while animals challenged with the CDT-defi

19 s and failed to mount IgG1 responses against H. hepaticus.

20 2 p40 from p50(-/-)p65(+/-) mice ameliorates H. hepaticus-induced disease.

21 Diversity among H. hepaticus isolates was evaluated by means of a restri

22 The urease genes among H. hepaticus strains were also well conserved, showing 9

23 An H. hepaticus transposon mutant with a disrupted cdtABC c

24 ma production by IL-10 KO CD4(+) cells in an H. hepaticus antigen-specific manner.

25 mice, were divided equally into control and H. hepaticus-infected groups and euthanized at 18 months

26 The results of H. hepaticus culture and H. hepaticus-specific PCR concurred (i.e., both positive

27 itric oxide synthase (iNOS) mRNA levels, and H. hepaticus-specific IFN-gamma secretion by mesenteric

28 9% of H. hepaticus-infected F1 male mice and H. hepaticus was isolated from hepatic tissues of all F1

29 ry properties of the CdtB of H. pullorum and H. hepaticus were assessed on human intestinal and hepat

30 oocytes has shown that UreI of H. pylori and H. hepaticus can transport urea only at acidic pH, where

31 tabolic PutA flavoenzymes from H. pylori and H. hepaticus revealed that Helicobacter PutA generates r

32 Infected mice also developed sustained anti-H. hepaticus serum immunoglobulin G antibody responses a

33 ng invasive adenocarcinoma, possibly because H. hepaticus, in delaying the development of colitis, al

34 Here we investigate the interactions between H. hepaticus and host immune cells that may promote mutu

35 Colonization of the mucosa by H. hepaticus was associated with a decrease in the overa

36 In contrast, H. hepaticus infection of mdr1a-/- mice did not accelera

37 A cdtB-deficient H. hepaticus isogenic mutant (HhcdtBm7) was generated an

38 assay was developed to quantitatively detect H. hepaticus in mouse ceca and feces using the ABI Prism

39 r-stained liver sections is used to diagnose H. hepaticus infection.

40 e that harbored or did not harbor endogenous H. hepaticus.

41 flammation in IL-10 KO mice with established H. hepaticus-induced colitis.

42 efect in the regulation of IL-12 expression, H. hepaticus induced markedly higher levels of IL-12 p40

43 red for the development of colitis following H. hepaticus infection.

44 2 p40 show no intestinal pathology following H. hepaticus infection.

45 In situ hybridization for H. hepaticus 16S rRNA showed that the bacteria was found

46 ths after inoculation by culture and PCR for H. hepaticus colonization of the liver and cecum, and mi

47 (p.i.) were surveyed by culture and PCR for H. hepaticus in liver and intestinal tissues.

48 Sera from mice positive for H. hepaticus by PCR or histologic examination (n = 88),

49 d the need to include diagnostic testing for H. hepaticus in a murine health monitoring program.

50 mesenteric lymph node (MLN) cells alone from H. hepaticus-infected mutant mice.

51 d that the cotransfer of CD4(+) T cells from H. hepaticus-infected but not uninfected WT mice prevent

52 ing this cytotoxic activity were cloned from H. hepaticus.

53 carrying the cloned cdtABC gene cluster from H. hepaticus reproduced the cytotoxic activities seen wi

54 rRNA gene and is biochemically distinct from H. hepaticus.

55 portion of the urease structural genes from H. hepaticus genomic DNA.

56 icus present in feces and cecal samples from H. hepaticus-infected mice was readily quantified.

57 uch lower sensitivity (10(6)-fold) than from H. hepaticus DNA.

58 ompared to 14 of 44 of the mice (32%) having H. hepaticus cultured from their frozen liver tumors.

59 Here we show that Helicobacter hepaticus (H. hepaticus), abundant in gut microbiota from rotenone-

60 However, H. hepaticus was not considered a complicating factor, b

61 However, H. hepaticus-infected BALB-RagMin mice had a significant

62 These lines of evidence indicate that (i) H. hepaticus CDT plays a crucial role in the persistent

63 The aim of this study was to determine if H. hepaticus will cause colitis in monoassociated mice l

64 In this study, we determined if H. hepaticus infection could prevent H. bilis-induced co

65 Hydrogenase activities in H. hepaticus were constitutive and not dependent on the

66 reviously described granulating cytotoxin in H. hepaticus.

67 st report profiling cecal gene expression in H. hepaticus-infected A/JCr mice.

68 ost infection by disrupting the putA gene in H. hepaticus.

69 tobacilli reduced intestinal inflammation in H. hepaticus-challenged IL-10-deficient mice despite sim

70 t of colonic, but not cecal, inflammation in H. hepaticus-infected anti-IL-10R-treated mice, demonstr

71 cant reduction of intestinal inflammation in H. hepaticus-infected IL-10(-/-) mice, suggesting an imp

72 suggesting a role for proline metabolism in H. hepaticus pathogenicity in vivo.

73 Two days after infection, H. hepaticus comprised a minor component of the mucosa-a

74 n forms of androgen interruption can inhibit H. hepaticus-induced hepatitis in young male mice, where

75 Rag-deficient hosts significantly inhibited H. hepaticus-induced inflammation and development of can

76 munoassays, and the quantities of intestinal H. hepaticus were evaluated by real-time PCR.

77 7BL/6 interleukin 10(-/-) mice with isogenic H. hepaticus mutants revealed that CDT expression is not

78 hepaticus in the ileum of female mice; (iv) H. hepaticus colonization was associated with down-regul

79 mice without characteristic hepatic lesions, H. hepaticus-specific DNA was amplified from the livers

80 Like H. hepaticus, it is a spiral bacterium with bipolar shea

81 d raises the possibility that H. canis, like H. hepaticus and H. bilis in mice, can cause inflammatio

82 PD fecal microbiota transplant or live H. hepaticus administration into antibiotics cocktail (A

83 ical bioassays conducted with B6C3F(1) mice, H. hepaticus has been regarded as a confounding factor b

84 By electron microscopy, H. hepaticus was noted infrequently and only in bile can

85 ic helicobacters, H. pylori and H. mustelae, H. hepaticus possesses a high level of urease activity.

86 The wild-type and putA mutant H. hepaticus strains displayed similar levels of infecti

87 However, a CDT-negative H. hepaticus mutant had a significantly diminished capac

88 Nongastric H. hepaticus produces urease similar to that of H. pylor

89 lastic liver lesions were observed in 69% of H. hepaticus-infected F1 male mice and H. hepaticus was

90 We have shown that although the ability of H. hepaticus to induce colitis in Rag-2(-/-) mice is inh

91 a critical role in inhibiting the ability of H. hepaticus to induce IL-12 p40.

92 Analysis of the gene expression in ceca of H. hepaticus infected mice revealed 25 up-regulated and

93 ucial role in the persistent colonization of H. hepaticus in SW mice; (ii) SW female mice are more re

94 d jejunum but only transient colonization of H. hepaticus in the ileum of female mice; (iv) H. hepati

95 The combination of H. hepaticus infection and CD45RB(high) CD4+ T-cell reco

96 ack of Th1-like ex-Th17 cells, the degree of H. hepaticus-triggered intestinal inflammation in mice i

97 this assay, the sensitivity for detection of H. hepaticus chromosomal DNA prepared from pure culture

98 vity is a potential virulence determinant of H. hepaticus that may play a role in the pathogenesis of

99 nscription factors during the development of H. hepaticus-associated liver tumors and may have releva

100 ess resulting from the relative dominance of H. hepaticus as a member of the community.

101 tope on the flagellar hook protein (FlgE) of H. hepaticus presented by I-Ab.

102 n study, suggesting that the urease genes of H. hepaticus are stable.

103 was conducted to determine the incidence of H. hepaticus infection and to evaluate different diagnos

104 ales, suggesting transplacental infection of H. hepaticus.

105 ction exist, and to analyze the influence of H. hepaticus on hepatocyte proliferation, a longitudinal

106 These results suggest that inhibition of H. hepaticus-induced IL-12 p40 expression by NF-kappaB s

107 Genomic DNAs from 11 isolates of H. hepaticus from the United States, Germany, France, an

108 The level of H. hepaticus serum antibody was highest in experimentall

109 igate the role of CDT in the pathogenesis of H. hepaticus, transposon mutagenesis was used to generat

110 nomodulatory role that allows persistence of H. hepaticus and that in IL-10-/- mice this alteration o

111 and was able to detect as little as 5 pg of H. hepaticus, H. bilis, or H. muridarum DNA.

112 n mice with a membrane digest preparation of H. hepaticus as the antigen.

113 tic approaches for assessing the presence of H. hepaticus in livers lacking characteristic lesions.

114 study confirms the widespread prevalence of H. hepaticus in mice, its potential to confound experime

115 deficient mice despite similar quantities of H. hepaticus in cocolonized animals.

116 The results of H. hepaticus culture and H. hepaticus-specific PCR concu

117 nosorbent assay (ELISA) for serodiagnosis of H. hepaticus infection in mice with a membrane digest pr

118 sitive and specific for the serodiagnosis of H. hepaticus infection in mice.

119 By PFGE, the genomic size of H. hepaticus is estimated to be roughly 1.3 Mb, which co

120 cytotoxic activities seen with sonicates of H. hepaticus.

121 oted in sequential isolates of one strain of H. hepaticus during an 18 month in vivo colonization stu

122 The putA mutant strain of H. hepaticus exhibited increased proline levels and resi

123 ated A/JCr mice with one of three strains of H. hepaticus: type strain Hh3B1, which contains the comp

124 ful in subsequent epidemiological studies of H. hepaticus when the source and method of spread of thi

125 ocyte proliferation, a longitudinal study of H. hepaticus-infected A/JCr mice was undertaken.

126 In contrast, anti-IL-17F had no effect on H. hepaticus-induced intestinal pathology.

127 epatocellular carcinoma was diagnosed in one H. hepaticus-infected mouse.

128 ne whether infection with either H. bilis or H. hepaticus would accelerate the development of inflamm

129 The deduced amino acid sequence of a partial H. hepaticus ureB gene product exhibited 75% identity an

130 e deduced amino acid sequence of the partial H. hepaticus ureA gene product was found to exhibit 60%

131 in the presence of a single murine pathogen, H. hepaticus.

132 ented colitis development despite persistent H. hepaticus infection in recipient mice.

133 s harbouring B. fragilis not expressing PSA, H. hepaticus colonization leads to disease and pro-infla

134 e to whole Helicobacter bacteria (H. pylori, H. hepaticus, and H. felis) was mediated not by TLR4 but

135 high sensitivity and specificity to quantify H. hepaticus in experimentally infected mouse models as

136 o obtain enzymatic activity from recombinant H. hepaticus urease; special conditions including NiCl2

137 ver tumors, argyrophilic bacteria resembling H. hepaticus were observed in liver sections, associated

138 In the absence of an intact IL-10 signaling, H. hepaticus induces an IL-23-driven inflammatory respon

139 d mesenteric lymph node cells with a soluble H. hepaticus antigen (Ag) preparation.

140 thogenic enterohepatic Helicobacter species, H. hepaticus.

141 Strong H. hepaticus-reactive antibody responses as measured by

142 ected animals throughout the 18-month study, H. hepaticus was consistently isolated from the lower bo

143 plenocyte populations are unable to suppress H. hepaticus-induced colitis in p50(-/-)p65(+/-)Rag-2(-/

144 phlonius was found to be less prevalent than H. hepaticus and H. rodentium but as prevalent as H. bil

145 Together, our data support the concept that H. hepaticus infection results in the induction in WT mi

146 We conclude that H. hepaticus does not induce or potentiate disease in ou

147 In this study we have demonstrated that H. hepaticus challenge of macrophages induces ERK activa

148 These findings indicate that H. hepaticus has urease structural genes which are homol

149 ys completed in our laboratory indicate that H. hepaticus is widespread in academic and commercial mo

150 d restriction enzyme analyses indicated that H. hepaticus and H. bilis infections are widespread in l

151 We here show that H. hepaticus Ag (SHelAg)-specific CD4+ Th1 clones transf

152 Our results show that H. hepaticus triggers early IL-10 induction in intestina

153 These results suggest that H. hepaticus induces ERK activation by a pathway depende

154 The data suggest that H. hepaticus, which is present in many research colonies

155 The H. hepaticus enzyme coupled H(2) oxidation to reduction

156 The H. hepaticus urease cluster contains a homolog of each g

157 The H. hepaticus urease gene cluster was expressed in Escher

158 The H. hepaticus-infected mouse will provide an ideal model

159 The aim of this study was to examine the H. hepaticus genome by pulsed-field gel electrophoresis

160 probe for this assay were generated from the H. hepaticus cdtB gene (encoding subunit B of the H. hep

161 However, the H. hepaticus urease structural gene sequences have not b

162 paticus cdtB gene (encoding subunit B of the H. hepaticus cytolethal distending toxin).

163 equivalent to approximately 14 copies of the H. hepaticus genome based on an estimated genome size of

164 timated by real-time PCR quantitation of the H. hepaticus-specific cytolethal distending toxin gene a

165 In this H. hepaticus-challenged IL-10-deficient murine colitis m

166 the presence of immunoglobulin G antibody to H. hepaticus and changes in the liver enzyme alanine ami

167 ve responses by splenic mononuclear cells to H. hepaticus antigens.

168 specific CD4+ Th1 clones transfer disease to H. hepaticus-infected T cell-deficient RAG KO hosts.

169 tis and the immune response of A/JCr mice to H. hepaticus infection.

170 Furthermore, prior exposure of donor mice to H. hepaticus significantly enhances antitumor potency of

171 onius is genetically most closely related to H. hepaticus.

172 e; (ii) SW female mice are more resistant to H. hepaticus colonization than male mice; (iii) there wa

173 eptible and C57BL/6NCr mice are resistant to H. hepaticus-induced hepatitis.

174 ich develop a significant immune response to H. hepaticus associated with prominent multifocal mononu

175 on of a cell-mediated Th1 immune response to H. hepaticus infection in the A/JCr mouse should prove v

176 s equipped to mount a successful response to H. hepaticus infection, increasing colon cancer risk.

177 patic gene expression profile in response to H. hepaticus infection.

178 To identify gene expression specific to H. hepaticus-induced hepatitis and progression to hepato

179 A/JCr mice are particularly susceptible to H. hepaticus-induced hepatitis and subsequent developmen

180 Only animals infected with wild-type type H. hepaticus developed significant typhlocolitis.

181 were experimentally infected with wild-type H. hepaticus and a CDT-deficient isogenic mutant.

182 Both wild-type H. hepaticus and the CDT-deficient mutant successfully c

183 Animals infected with wild-type H. hepaticus developed serum immunoglobulin G1 (IgG1) an

184 Animals infected with wild-type H. hepaticus exhibited severe typhlocolitis at 8 months

185 tectable in IL-10-/- mice, whereas wild-type H. hepaticus persisted for the 8-month duration of the e

186 rechallenge with either mutant or wild-type H. hepaticus.

187 To analyze whether H. hepaticus persists in specified ecological niches, to

188 lis accelerates development of colitis while H. hepaticus delays disease.

189 n wild-type macrophages after challenge with H. hepaticus.

190 obacillus paracasei and then challenged with H. hepaticus.

191 Coinfection with H. hepaticus also suppressed H. pylori-induced elevation

192 ions (59%) were most commonly colonized with H. hepaticus alone or in combination with other Helicoba

193 t have histological features compatible with H. hepaticus infection.

194 erleukin-4 (IL-4) or IL-5 when cultured with H. hepaticus outer membrane proteins.

195 anling A/JCr mice (n = 67) were gavaged with H. hepaticus or vehicle.

196 Mice were gavaged with H. hepaticus; DFO feeding was continued; and mice were s

197 However, mice infected with H. hepaticus and H. pylori (HhHp mice) developed more se

198 contact mice were persistently infected with H. hepaticus as identified by culture and PCR, in both t

199 mice; all were confirmed to be infected with H. hepaticus by culture and PCR.

200 Mice infected with H. hepaticus developed progressively severe perivascular

201 Male A/JCr mice were infected with H. hepaticus or vehicle at 4 weeks and randomized into s

202 rt that hepatitis of male mice infected with H. hepaticus show significant increases in the oxidative

203 ntact mice became persistently infected with H. hepaticus, lesions were less severe and the levels of

204 s and carcinogenesis in livers infected with H. hepaticus.

205 Concurrent infection with H. hepaticus may confound studies that have been attribu

206 Infections with H. hepaticus and H. bilis have been associated with hepa

207 cking the p50/p105 subunit of NF-kappaB with H. hepaticus.

208 with natural infection of SCID/NCr mice with H. hepaticus and that lesions are progressive with age.

209 ins, derived from A/J and C57BL/6 mice, with H. hepaticus to determine the genetic basis of resistanc

210 genetic background were monoassociated with H. hepaticus ATCC 51448 by oral feeding and rectal enema

211 und that IL-10(-/-) mice monoassociated with H. hepaticus for up to 16 weeks showed almost no histolo

212 had mice that were either monoinfected with H. hepaticus, monoinfected with seven other Helicobacter

213 10 C57BL/6 mice were inoculated per os with H. hepaticus, Helicobacter muridarum, or H. bilis.

214 tibody to heat shock protein 70 reacted with H. hepaticus in tissue sections and to a H. hepaticus pr

215 they responded when stimulated in vitro with H. hepaticus and Helicobacter typhlonius Ag, but not whe

216 retion, or T-cell activation with or without H. hepaticus.

217 ) in females (P < 0.016 and 0.031 between WT H. hepaticus-infected and sham-dosed females, respective

218 in both female and male mice colonized by WT H. hepaticus or in males transiently colonized through 8

219 iii) there was persistent colonization of WT H. hepaticus in cecum, colon, and jejunum but only trans

220 d 16 wpi; however, colonization levels of WT H. hepaticus in the cecum and colon of male mice were ap

221 vely) responses in the mice infected with WT H. hepaticus when compared to HhcdtBm7 at 16 wpi.

222 Infection with WT H. hepaticus, but not HhcdtBm7, at 8 wpi was associated

223 Wild-type (WT) H. hepaticus was detected in the corresponding intestina