ライフサイエンスコーパス: Holliday junction

1 is relaxed can RuvAB bind to a RecG-extruded Holliday junction.

2 DNA structure in these two processes is the Holliday junction.

3 ecules connected to each other by a flexible Holliday junction.

4 rently due to cleavage of the regressed fork Holliday junction.

5 ubstrates and include the D-loop and partial Holliday junction.

6 ite-specific double-stranded end (DSE) and a Holliday junction.

7 romoting second end capture to form a double Holliday junction.

8 s of stiffness at the locations of nicks and Holliday junctions.

9 bundle also inhibits the cleavage of intact Holliday junctions.

10 m duplex, such as fork structures and nicked Holliday junctions.

11 nd exchange and promotes branch migration of Holliday junctions.

12 trates, while the latter exclusively targets Holliday junctions.

13 (mEndoG) shows specificity for both 5hmC and Holliday junctions.

14 nforced when the protein was shown to unwind Holliday junctions.

15 involve the coordinated cleavage of classic Holliday junctions.

16 stall at UV lesions and regress, generating Holliday junctions.

17 omplex) to promote the dissolution of double Holliday junctions.

18 ctivated in meiosis II to resolve persistent Holliday junctions.

19 strand exchanges through branch migration of Holliday junctions.

20 'late' roles, such as dissolution of double Holliday junctions.

21 ture-specific endonucleases that cleave most Holliday junctions.

22 ly branched DNA intermediates containing few Holliday junctions.

23 to form four-stranded structures resembling Holliday junctions.

24 NA structures that include forks, flaps, and Holliday junctions.

25 cts the symmetric cleavage and resolution of Holliday junctions.

26 anched four-stranded DNA structures known as Holliday junctions.

27 ry structures at the replication fork and at Holliday junctions.

28 owed that Rad54 promotes branch migration of Holliday junctions.

29 ectly convert stalled replication forks into Holliday junctions.

30 r modification state is negligibly active on Holliday junctions.

31 at are organized and dictated by a series of Holliday junctions.

32 imic B-DNA, to predict the properties of DNA Holliday junctions.

33 for its unique ability to decatenate double-Holliday junctions.

34 h affinity and shows a marked preference for Holliday junctions.

35 ppa joints, synthetic replication forks, and Holliday junctions.

36 exchange of DNA between chromatids, forming Holliday junctions.

37 ts limited endonuclease activity with intact Holliday junctions.

38 XO1 and MutSgamma, the latter of which binds Holliday junctions(11).

39 recombination intermediates known as double Holliday junctions(2,3).

40 roximately 1 min(-1)) and include the nicked Holliday junction, 3'-flapped and replication fork-like

41 omplex designs including linear, bifurcated, Holliday junction, 8-arm star and dendrimers involving u

42 branched structures other than the canonical Holliday junction, a feature advantageous for viral DNA

43 own to catalyze branch migration (BM) of the Holliday junction, a key intermediate in DNA repair and

44 nzymes include the ability to resolve double Holliday junctions, a process thought to lead to the sup

45 tly probe the conformational ensemble of the Holliday junction across a wide range of ionic condition

46 sW and UvsW-K141R are capable of stabilizing Holliday junctions against spontaneous branch migration

47 es is determined by the effective density of Holliday junctions along a particular stress direction.

48 lly, Red1 preferentially associated with the Holliday junction and 3-way junction rather than with si

49 tS homologs, hMSH4-hMSH5, bind uniquely to a Holliday Junction and its developmental progenitor.

50 ing protein exhibiting high affinity for the Holliday junction and promoting DNA bridging, condensati

51 tion and six were found to confer defects in Holliday junction and replication fork cleavage in vivo.

52 ption of the conformational landscape of the Holliday junction and underscore the utility of XSI for

53 the Mut S homolog 4 (Msh4) and Msh5 bind to Holliday junctions and are required for homologous recom

54 induce ATP-dependent unwinding of synthetic Holliday junctions and ATP-stimulated annealing of uncon

55 activation does not induce a preference for Holliday junctions and does not induce multimerization o

56 EM visualization of BCDX2 and CX3 binding to Holliday junctions and forked DNAs and suggests the comp

57 Aberrant JMs contain high levels of single Holliday junctions and include intersister JMs, multichr

58 is capable of binding three-way and four-way Holliday junctions and model replication forks that lack

59 show SLX1-dependent nuclease activity toward Holliday junctions and MUS81-dependent activity toward o

60 le nucleases in order to effectively resolve Holliday junctions and repair interstrand crosslinks (IC

61 NCM also catalyzes branch migration of model Holliday junctions and replication forks in vitro.

62 e active site is responsible for cleavage on Holliday junctions and splayed duplexes.

63 s, which resulted in processing of synthetic Holliday junctions and stabilization of model replicatio

64 all three dimensions facilitated by immobile Holliday junctions and sticky end cohesion.

65 p3 inhibited the Hel112 helicase activity on Holliday junctions and stimulated formation and stabiliz

66 tion on two major activities of RAD54, BM of Holliday junctions and stimulation of DNA pairing.

67 sion intermediates are converted into double Holliday junctions and then resolved into crossovers tha

68 eaves 3'-flaps, replication forks and nicked Holliday junctions, and exhibits limited endonuclease ac

69 cterization of fluorescent nanodiamonds, DNA Holliday junctions, and protein-DNA interactions.

70 ding of homologous pairing, and migration of Holliday junctions, and they suggest that various functi

71 Holliday junctions are critical intermediates in DNA rec

72 iple of crossover resolution is that the two Holliday junctions are resolved in opposite planes by ta

73 obeams with specified densities of nicks and Holliday junctions are synthesized and stretched by flui

74 Resolvase enzymes that cleave DNA four-way (Holliday) junctions are required for poxvirus replicatio

75 Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombi

76 The single-crystal structure of a DNA Holliday junction assembled from four unique sequences s

77 Holliday junctions assembled from oligonucleotides with

78 In both conditions, Holliday junctions at DNA break hotspots form more frequ

79 ependent, X-shaped DNA structures containing Holliday junctions between sister chromatids.

80 Our kinetic studies of Holliday junction branch migration catalysed by a ring-s

81 etic evidence, has been proposed to act as a Holliday junction branch migration enzyme.

82 sses such as replication fork regression and Holliday junction branch migration.

83 nt protein, UvsW-K141R, is unable to promote Holliday junction branch migration.

84 n of DSBs and then defines the boundaries of Holliday junction branch migration.

85 as at most a minor role in resolution of the Holliday junction but acts as an essential backup to MUS

86 MUS81-EME1, SLX1-SLX4 and GEN1 also process Holliday junctions but, in contrast to the BTR complex,

87 also binds various branched DNAs, including Holliday junctions, but does not show canonical resolvas

88 ase ensemble preferentially cleaves DNA with Holliday junctions, but shows no canonical resolvase act

89 Recombinant GEN1 and Yen1 resolve Holliday junctions by the introduction of symmetrically

90 RecA-generated Holliday junctions can be detected by RuvC cleavage, alt

91 In somatic cells, Holliday junctions can be formed between sister chromati

92 involves replication fork regression into a Holliday junction ('chicken foot structure'), DNA synthe

93 ructural similarity is found to the archaeal Holliday junction cleavage enzymes, strongly suggesting

94 Holliday junction cleavage requires the coordination of

95 clease complex [gene product (gp) 46/47] and Holliday junction-cleaving enzyme (EndoVII), respectivel

96 e and RuvC, and a model of the CPV resolvase.Holliday junction complex provides insights into the con

97 wever, structural superposition on bacterial Holliday junction complexes and similarities in the C-te

98 taining bubbles of unpaired nucleotides, and Holliday junction constructs in vitro with dissociation

99 the final step of homologous recombination, Holliday junction-containing joint molecules (JMs) are r

100 Using a novel Holliday junction-containing substrate, we also demonstr

101 The active form of the enzyme on the Holliday junction contains a single RuvA tetramer.

102 Our results indicate that Holliday junctions control mechanical behaviors of DNA n

103 molecules, a protein-DNA complex and dynamic Holliday junctions demonstrate the potential of switchab

104 pha) is an essential component of the double Holliday junction (dHJ) dissolvasome complex in metazoan

105 olliday junction (HJ) or dissolve the double Holliday junction (dHJ) structure to yield non-crossover

106 This complex can resolve the double Holliday junction (dHJ), a DNA intermediate generated du

107 The double Holliday junction (DHJ), presupposed to be the central i

108 formation via the dissolution of the double Holliday Junction (dHJ).

109 a homology search, ultimately forming double Holliday junctions (dHJs) that primarily include both ho

110 tion of crossover-specific JMs called double Holliday junctions (dHJs).

111 , branch migration, and resolution of double Holliday junctions (dHJs).

112 and MutL, may utilize its ability to unwind Holliday junctions directly in the prevention of homeolo

113 rement for the BLM ATPase activity in double Holliday junction dissolution and DNA unwinding by const

114 two stages: 5' DNA end resection and double Holliday junction dissolution.

115 nalyse human cells compromised for the known Holliday junction dissolution/resolution pathways.

116 in the disease biochemically functions as a Holliday junction dissolvase and human cells lacking fun

117 domains to the four "core-type" sites of the Holliday junction DNA and via its amino-terminal domains

118 d the "dissolvasome," which separates double-Holliday junction DNA structures that can arise during D

119 and breaks and also in the resolution of the Holliday junction during homologous recombination.

120 for crossover-specific resolution of double Holliday junctions during meiosis.

121 in independent pathways that branch migrate Holliday junctions during recombinational DNA repair.

122 le, we studied the effect of the sequence on Holliday junction dynamics and branch migration process.

123 een applied to new experimental FRET data of Holliday Junction dynamics, extracting the expected two

124 e that UvsW promotes the branch migration of Holliday junctions efficiently through more than 1000 bp

125 Holliday junctions enable modification with multiple flu

126 Mlh1-Mlh3 prefers to bind the open unstacked Holliday junction form.

127 trate that WRN catalyzes fork regression and Holliday junction formation.

128 break repair pathway and that resolution of Holliday junctions formed as part of the latter pathway

129 pair during replication and can also resolve Holliday junctions formed during homologous recombinatio

130 wed the reaction intermediate transitions as Holliday junctions formed through catalysis by XerD, iso

131 is more efficient at generating the initial Holliday junction from the stalled fork.

132 nd lagging strands, whereas RuvC cleaves the Holliday junction generated by RecG-catalyzed fork regre

133 can be produced, including migratable single Holliday junctions, hemicatenanes and a quadruple Hollid

134 cG helicase of Escherichia coli unwinds both Holliday junction (HJ) and replication fork DNA substrat

135 interacts with DNA, using an engineered DNA Holliday junction (HJ) as a protein-DNA interaction repo

136 forces on the nanometer scale motion of the Holliday junction (HJ) at 100-hertz bandwidth.

137 The assembly is a 240-bp Holliday junction (HJ) bound specifically by 11 protein

138 role in DNA unwinding, strand annealing, and Holliday junction (HJ) branch migration.

139 ologous recombination, from end resection to Holliday junction (HJ) dissolution.

140 nd this domain preferentially binds fork and Holliday junction (HJ) DNA in vitro and is required for

141 ed in the mus81Delta mutant contain a single Holliday junction (HJ) explaining why STR is unable to p

142 in, FtsK, which activates the formation of a Holliday Junction (HJ) intermediate by XerD catalysis th

143 ter which a pair of strand exchanges forms a Holliday junction (HJ) intermediate; HJ isomerization th

144 y Rec12 (Spo11 homolog) and generates single Holliday junction (HJ) intermediates, which are resolved

145 recombination intermediates (Rec-Xs) but not Holliday junction (HJ) intermediates.

146 hase, by polo kinase-triggered resolution of Holliday junction (HJ) intermediates.

147 The Holliday junction (HJ) is a central intermediate in homo

148 The Holliday junction (HJ) is a central intermediate of vari

149 Holliday junction (HJ) is a hallmark intermediate in DNA

150 BLM-Topo IIIalpha pair to branch migrate the Holliday junction (HJ) or dissolve the double Holliday j

151 Holliday junction (HJ) resolution is essential for chrom

152 Holliday junction (HJ) resolution is required for segreg

153 nhibits telomere t-loop deletion by blocking Holliday junction (HJ) resolvase cleavage activity, but

154 Holliday junction (HJ) resolvases are necessary for the

155 Holliday junction (HJ) resolvases are structure-specific

156 the ruvC and yqgF genes that encode putative Holliday junction (HJ) resolvases.

157 Holliday junction (HJ) resolving enzyme RecU is involved

158 The Holliday junction (HJ), a cross-shaped structure that ph

159 The Holliday junction (HJ), or four-way junction, is a centr

160 ge of branched DNA structures, including the Holliday junction (HJ), with little sequence-specificity

161 f four-way DNA structures referred to as the Holliday junction (HJ).

162 m's strand-exchange mechanism proceeds via a Holliday-junction (HJ) intermediate; however, the geomet

163 tures are consistent with an achiral, planar Holliday-junction (HJ) structure, whereas topological as

164 Holliday junctions (HJs) are DNA intermediates in homolo

165 Holliday junctions (HJs) are four-way DNA intermediates

166 Holliday junctions (HJs) are key DNA intermediates in ho

167 Holliday junctions (HJs) are physical links between homo

168 Holliday junctions (HJs) are X-shaped DNA structures tha

169 on of recombination intermediates containing Holliday junctions (HJs) is critical for genome maintena

170 ences between the exchange sites to generate Holliday junctions (HJs) that contain mismatched bases.

171 generalizable method of using engineered DNA Holliday junctions (HJs) that contain specific protein-r

172 Holliday junctions (HJs) that physically link sister chr

173 symmetrical cleavage of static and migrating Holliday junctions (HJs), identifying SLX1-SLX4 as a HJ

174 are endonucleases with the ability to cleave Holliday junctions (HJs), which are proposed intermediat

175 wn to have high affinity and specificity for Holliday junctions (HJs).

176 hromosomes are covalently linked by four-way Holliday junctions (HJs).

177 These fluorescent Holliday junctions improve fluorescence yields for both

178 gulate recombination by disassembling double Holliday junctions in a process called dissolution.

179 Rad52-promoted annealing in the formation of Holliday junctions in DSB repair.

180 ast to their free-solution conformation, the Holliday junctions in the DNA origami structures adopt a

181 results from stabilization of peptide-bound Holliday junctions in the square planar conformation.

182 Mus81-Mms4 nuclease activity on 3' flaps and Holliday junctions in vitro but that activation does not

183 t harbors two Holliday junctions (the double Holliday junction) in a non-crossover manner.

184 Hence, Holliday junction incision activity in vitro cannot be a

185 tner site) not only allowed formation of the Holliday junction intermediate but also increased the ra

186 of complexes between the recombinase and the Holliday junction intermediate of several highly diverge

187 f stalled replication forks by unwinding the Holliday junction intermediate to allow bypass of the bl

188 ifsy-1, Gifsy-2, Fels-1, Fels-2) and to trap Holliday junction intermediates of phage lambda site-spe

189 ctivates XerD catalytic activity to generate Holliday junction intermediates that can then be resolve

190 plication fork reversal via the formation of Holliday junction intermediates, suggesting that RuvAB-c

191 on and crossover-biased resolution of double-Holliday Junction intermediates.

192 tion in a complex proposed to resolve double-Holliday-junction intermediates into crossovers during m

193 A templates containing replication forks and Holliday junctions, intermediates observed during DNA re

194 A templates containing replication forks and Holliday junctions, intermediates observed during DNA re

195 The Holliday junction is a central intermediate in various g

196 The Holliday junction is known to have an antiparallel orien

197 Significantly, a substrate containing a Holliday junction is unwound most efficiently.

198 omplex and show that preferential binding to Holliday junctions is a conserved capacity of eukaryotic

199 Processing of Holliday junctions is essential in recombination.

200 The DNA four-way (Holliday) junction is the central intermediate of geneti

201 The four-way junction (4WJ), or Holliday junction, is a dynamic DNA structure involved i

202 proteins may cooperate in the processing of Holliday junction-like intermediates during homologous r

203 Mus81-Eme1 endonuclease activity on various Holliday junction-like intermediates.

204 randed DNA junctions (Holliday junctions) or Holliday junction-like structures containing three of th

205 can ensure coordinate, bilateral cleavage of Holliday junction-like structures.

206 he assembly of a 3D scaffold via a series of Holliday junctions linked together with complementary st

207 rand annealing, rather than through a double Holliday Junction mechanism.

208 ermediates such as D-loops and static X-DNA (Holliday junction mimic) to ssDNA products is enhanced b

209 synthetic oligonucleotides via a four-armed Holliday junction motif.

210 rred regressed fork substrate for RuvAB is a Holliday junction, not a forked DNA.

211 m where a single-stranded DNA nanostructure (Holliday junction or paranemic cross-over DNA) is insert

212 ght explain crossover-specific processing of Holliday junctions or their precursors in meiotic chromo

213 to be active on four-stranded DNA junctions (Holliday junctions) or Holliday junction-like structures

214 such as displacement loops (D-loops), nicked Holliday junctions, or 3' flaps but not intact Holliday

215 ting a previously unappreciated affinity for Holliday junctions over other substrates.

216 Holliday junctions play a central role in genetic recomb

217 hat normally replicating human cells require Holliday junction processing activities to prevent siste

218 We provide insights into the mode of Holliday junction recognition and show that Mlh1-Mlh3 pr

219 ase by the CENP-A-specific histone chaperone Holliday junction recognition protein (HJURP) coupled wi

220 serve that the centromere-specific chaperone Holliday Junction Recognition Protein (HJURP) stabilizes

221 otein A), deposited by its chaperone, HJURP (Holliday junction recognition protein).

222 t ADP-->ATP exchange is uniquely provoked by Holliday Junction recognition.

223 s Escherichia coli host chromosome through a Holliday junction recombination intermediate.

224 ted chemical cross-linking of Int in trapped Holliday junction recombination intermediates and recomb

225 omic structure of a resolvase complex with a Holliday junction remained elusive.

226 fission yeast mus81Delta strains results in Holliday junction resolution and crossover formation dur

227 ng yeast polo-like kinase, is sufficient for Holliday junction resolution and exit from pachytene.

228 RAD51C) participates in branch migration and Holliday junction resolution and thus is important for p

229 human GEN1 protein and show that it promotes Holliday junction resolution by a mechanism that is anal

230 papers suggest that MUS312 and BTBD12 direct Holliday junction resolution by at least two distinct en

231 e XPG-family endonuclease GEN1 that promotes Holliday junction resolution in vitro, suggesting that i

232 yces cerevisiae and human cells that promote Holliday junction resolution, in a manner analogous to t

233 inates the SLX1 and MUS81-EME1 nucleases for Holliday junction resolution, in a reaction stimulated b

234 s, topoisomerase I (TOP1) inhibitors, and in Holliday junction resolution.

235 ologous strand exchange and RuvABC-catalyzed Holliday junction resolution.

236 ddressing long-standing questions related to Holliday junction resolution.

237 gene fusion library for nucleases capable of Holliday junction resolution.

238 is HP0334 (dprB), annotated to be a putative Holliday junction resolvase (HJR).

239 Mlh3 may not act like a canonical, RuvC-like Holliday junction resolvase and support a novel model in

240 re resolved prior to cell division, the Yen1 Holliday junction resolvase is activated at anaphase.

241 The virus-encoded Holliday junction resolvase is required to process conca

242 logous to that shown by the Escherichia coli Holliday junction resolvase RuvC.

243 atemer substrate by employing a portal-bound Holliday junction resolvase that trims and releases thes

244 They also need RecA recombinase and a Holliday junction resolvase to survive rapid growth, but

245 dc14 and, using this insight, identified the Holliday junction resolvase Yen1 as a DNA repair target

246 Additionally, we identify GEN-1, a Holliday junction resolvase, as an evolutionarily conser

247 The human Holliday junction resolvase, GEN1, and its yeast ortholo

248 Another nuclease, the Mus81-Eme1 Holliday junction resolvase, is required to generate cro

249 leases and has been recently identified as a Holliday junction resolvase, results in increased sensit

250 Therefore, the yeast CCE1 gene, encoding a Holliday junction resolvase, was introduced into cells c

251 contains two domains, a third OB fold and a Holliday junction resolvase-like domain.

252 ized by the element protein and cleaved in a Holliday junction resolvase-like reaction.

253 the gammaH2AX-binding protein Brc1 and Mus81 Holliday junction resolvase.

254 d-break repair and SOS induction, and RuvABC Holliday-junction resolvase.

255 Viral and bacterial Holliday junction resolvases differ in specificity with

256 tructure provides insights into how archaeal Holliday junction resolvases evolved to incise 5' flap s

257 Several Holliday junction resolvases have since been characteriz

258 The eukaryotic Holliday junction resolvases represent a new subclass of

259 mosomal compaction, topoisomerase action and Holliday junction resolvases, ensure that all SCIs are r

260 ly of its nuclear role as a component of the Holliday junction-resolving BLM-Top3alpha-RMI1-RMI2 (BTR

261 cise structurally distinct bubbles, ends, or Holliday junctions, respectively.

262 eins responsible for branch migration of the Holliday junction(s) and reversal of stalled replication

263 The immobile Holliday junction sequence allowed us to produce crystal

264 Quantification of Holliday junctions showed that while recombination at ce

265 junction, and coupling required the complete Holliday junction structure.

266 n the ability to unwind replication fork and Holliday junction structures but has no effect on substr

267 ults indicate that RecG and RuvB compete for Holliday junction structures in recombinational repair,

268 oreover, the DprB protein binds to synthetic Holliday junction structures rather than double-stranded

269 catalyzes unwinding of replication fork and Holliday junction structures.

270 lyzes coordinate bilateral cleavage of model Holliday-junction structures.

271 wed that cleavage of a fluorescently labeled Holliday junction substrate did not yield an appreciable

272 nd reduced unwinding activity on a synthetic Holliday junction substrate relative to full-length GcRe

273 ingle turnover kinetic analysis revealed the Holliday junction substrate was cleaved 90-fold faster t

274 near DNA, and had the highest affinity for a Holliday junction substrate, illustrating a previously u

275 r creating a novel DNA substrate, the double Holliday junction substrate.

276 day junctions, hemicatenanes and a quadruple Holliday junction substrate.

277 eveal that recognition of 3'-flap and nicked Holliday junction substrates by Mus81-Mms4 involves indu

278 UvrD also catalyzes the robust unwinding of Holliday junction substrates.

279 and actively resolves telomeric D-loops and Holliday junction substrates.

280 mi with rotational symmetric arrangements of Holliday junctions, such as those in DNA nanotubes.

281 This substrate contains two Holliday junctions that are mobile, topologically constr

282 Strand invasion events mature into double Holliday junctions that can be resolved as crossovers.

283 y question is whether Mus81-Mms4 can process Holliday junctions that contain four uninterrupted stran

284 Holliday junctions that contain identical sequences betw

285 t a role for UvsW in the branch migration of Holliday junctions that form during T4 recombination, re

286 tly resolve a DNA substrate that harbors two Holliday junctions (the double Holliday junction) in a n

287 ase can dissolve DNA conjoined with a double Holliday junction, thus reducing DNA linkage.

288 ation involves the nucleolytic resolution of Holliday junctions to generate crossovers.

289 phores installed in close proximity, we used Holliday junctions to label proteins site-specifically.

290 A variety of processes act upon Holliday junctions to remove them from DNA, in events th

291 formation and preferential migration of the Holliday junctions to the boundaries of sequence homolog

292 ubstituted by Escherichia coli Top3, and the Holliday junction unwinding activity of BLM-related heli

293 In vitro, Rad54 promotes branch migration of Holliday junctions, whereas the Mus81-Eme1 complex resol

294 The inverted repeats refold to generate Holliday junctions, which are cleaved by the virus-encod

295 ibed to the collective actions of individual Holliday junctions, which are only possible in DNA origa

296 stabilizes invasion intermediates and double Holliday junctions, which are resolved into crossovers i

297 We designed a Holliday junction with three of its four arms modified w

298 Crucially, these two variants cleaved Holliday junctions with enhanced specificity and symmetr

299 lliday junctions, or 3' flaps but not intact Holliday junctions with four uninterrupted strands.

300 sired nanostructure by DNA staples that form Holliday junctions with the template.