ライフサイエンスコーパス: IBDV

1 In addition, IBDV-induced bursal T cells produced elevated levels of

2 acterized cellular defense mechanism against IBDV infections.

3 transcripts derived from IPNV segment A and IBDV segment B and Vero cells were transfected with tran

4 cell line DT40 to rescue a panel of chimeric IBDVs and perform neutralization assays.

5 en B-cell line to rescue a panel of chimeric IBDVs with the HVR from seven diverse IBDV field strains

6 oreign peptides from a replication-competent IBDV and demonstrates the potential of this virus as a v

7 We demonstrated IBDV induction of autophagy as a significant increase in

8 he ability of vaccines to neutralize diverse IBDV genogroups and to better understand the relationshi

9 valuate the antigenic relatedness of diverse IBDV strains, and when coupled with structural modeling,

10 imeric IBDVs with the HVR from seven diverse IBDV field strains and to conduct neutralization assays

11 Induction of autophagy enhances IBDV replication, whereas inhibition of autophagy impair

12 ction, and the autophagy pathway facilitates IBDV replication complex function and virus assembly, wh

13 proposed as a new mechanism that facilitates IBDV maturation, release, and reinternalization.

14 -based reverse-genetics system developed for IBDV, we generated five chimeric viruses after transfect

15 PurSA activity is thus essential for IBDV replication.

16 tidase activity in cell lines permissive for IBDV replication caused a major blockade in assembly and

17 appaB) is also activated and is required for IBDV-induced apoptosis.

18 development of live-attenuated vaccines for IBDV.

19 re transfected with transcripts derived from IBDV segment A and IPNV segment B.

20 diated release of progeny virions.IMPORTANCE IBDV is the most extensively studied virus in terms of m

21 In IBDV-infected chickens, bursal T cells proliferated in v

22 racteristics of intrabursal T lymphocytes in IBDV-infected chickens and examined whether T cells were

23 immunofluorescence studies, we found that in IBDV-infected cells the mainly nuclear NF45 accumulated

24 in assembly and/or maturation of infectious IBDV particles, as virus yields were reduced markedly.

25 -kappaB inhibitor MG132 completely inhibited IBDV-induced DNA fragmentation, caspase 3 activation, an

26 ), we observed that a large number of intact IBDV virions were arranged in a lattice surrounded by p6

27 erved epitopes to strategically design novel IBDV vaccines in the future.

28 sis, increased viral burden in the bursae of IBDV-infected chickens.

29 Spleen and bursal cells of IBDV-infected chickens had upregulated gamma interferon

30 pathogenesis, we constructed a cDNA clone of IBDV segment A in which the first and only initiation co

31 sease virus (IBDV) VP3, a major component of IBDV ribonucleoprotein complexes, on the regulation of V

32 in replication in vivo, it induced levels of IBDV neutralizing antibodies that were similar to those

33 irulence and pathogenic-phenotype markers of IBDV reside in VP2.

34 ral replication and examine the mechanism of IBDV-induced apoptosis.

35 iruses to track the location and movement of IBDV VFs, in order to better understand the intracellula

36 of the parental attenuated vaccine strain of IBDV (D78) in vitro.

37 ckens were exposed to a pathogenic strain of IBDV (IM), the virus rapidly destroyed B cells in the bu

38 e, pathogenic phenotype, and cell tropism of IBDV, we prepared full-length cDNA clones of a virulent

39 In either case, no infectious IPNV or IBDV particles were generated even after a third passage

40 ated in vitro upon stimulation with purified IBDV in a dose-dependent manner (P<0.02), whereas virus-

41 empts were also made to generate recombinant IBDV that displayed foreign epitopes in the exposed loop

42 We successfully recovered recombinant IBDVs expressing c-Myc and two different virus-neutraliz

43 d neutralization assay can be used to screen IBDV vaccine candidates, platforms, and regimens for the

44 We also demonstrated that IBDV infection induced autophagosome-lysosome fusion, bu

45 ed a baculovirus-based system to express the IBDV polyprotein in insect cells and found inefficient f

46 the 3' untranslated regions (UTRs) from the IBDV genome segments.

47 of the study was to identify regions in the IBDV genome that are amenable to the introduction of a s

48 ogenesis; however, mechanisms underlying the IBDV life cycle require further exploration.

49 two lines that differ in their resistance to IBDV infection.

50 candidates for involvement in resistance to IBDV.

51 formation of virus-like particles similar to IBDV virions, which correlates with the absence of purom

52 ment A, and with segment B, generated viable IBDV progeny.

53 ropism, and pathogenic phenotype of virulent IBDV.

54 ody 21, which binds specifically to virulent IBDV, the putative amino acids involved in virulence and

55 Using the Infectious Bursal Disease Virus (IBDV) as a model, we validate that the viral protein 3 (

56 recombinant infectious bursal disease virus (IBDV) expressing split-GFP11 tagged to the polymerase (V

57 Eight infectious bursal disease virus (IBDV) genogroups have been identified based on the seque

58 ipts of the infectious bursal disease virus (IBDV) genome, was recently developed.

59 Infectious bursal disease virus (IBDV) is an avian lymphotropic virus that causes immunos

60 role of the infectious bursal disease virus (IBDV) VP3, a major component of IBDV ribonucleoprotein c

61 genic avian infectious bursal disease virus (IBDV) was explored.

62 this study, infectious bursal disease virus (IBDV) was used to investigate the role of autophagy in a

63 VP2) of the infectious bursal disease virus (IBDV), a double-stranded RNA virus, is processed at the

64 fe cycle of infectious bursal disease virus (IBDV), a double-stranded RNA virus, we examined the cell

65 of IPNV and infectious bursal disease virus (IBDV), another birnavirus, can support virus rescue in h

66 Infectious bursal disease virus (IBDV), the causative agent of a highly contagious diseas

67 nfection by infectious bursal disease virus (IBDV).

68 aviruses, infectious bursal disease viruses (IBDVs) containing a split GFP11 or tetracysteine (TC) ta

69 combinant infectious bursal disease viruses (IBDVs) of strain PBG98 containing either a split GFP11 o

70 Infectious bursal disease viruses (IBDVs), belonging to the family Birnaviridae, exhibit a