ライフサイエンスコーパス: IEF

1 IEF was performed in a mixture of four small and chemica

2 IEF/SDS-PAGE examination of irradiated tadpole brain hom

3 IEF/SDS/PAGE can precisely differentiate protein isoform

4 DS PAGE)-LA ICP MS, and, in duplicate, by 2D IEF-PAGE.

5 compared to one-dimensional SPLC-MS, the 2D IEF-SPLC-MS platform resulted in a 5-6x increase in the

6 The proteomic analysis was done using 2D-IEF SDS PAGE and nano-LC-MS/MS.

7 To adapt IEF using IPGs to widely used microfluidic device materi

8 Depending on the sensor matrix, they allowed IEF within a pH range of roughly 5.5-10.5 with good sens

9 ally useful for evaluating the quality of an IEF or NEPHGE tube gel before using it for the second di

10 Early enteral feeding with an IEF has been associated with improved outcome in trauma

11 Early enteral feeding with an IEF was not beneficial and should not be used in a routi

12 nel passivation, electroosmosis control, and IEF linearity control.

13 Continuum solvation methods (SMD and IEF-PCM) and the MPWB1K functional are used.

14 lumes of interest from the IEF dimension, as IEF was 'parked' during each CE analysis and refocused p

15 inducible phosphoprotein STI1 (also known as IEF SSP 3521 or p60).

16 heterogeneous separation techniques, such as IEF, capillary electrophoresis, and liquid chromatograph

17 included B3LYP, MP2, and CBS-QB3, as well as IEF-PCM to approximate implicit water solvation.

18 versus CA-IEF), and 1.4 mum/min in mixed-bed IEF (~43-fold reduction versus CA-IEF).

19 Lastly, mixed-bed IEF in a PDMS device resolved green fluorescent protein

20 d formulation of IPG gels and CAs (mixed-bed IEF).

21 focusing (IEF) and in situ antibody blotting IEF was employed to monitor dark (nonfluorescent) and br

22 n creates PA gels that are suitable for both IEF and subsequent in-gel immunoprobing by mitigating im

23 These findings were confirmed by IEF coupled with size exclusion chromatography on Supero

24 tablish the tunable pH gradients required by IEF and precisely control the transport and handling of

25 Of the 10 isoforms of eIF4E-BP1 resolved by IEF/SDS/PAGE, at least seven are labelled with [32P] and

26 borns from expensive confirmatory testing by IEF, without missing any of the SCD newborns in the stud

27 Analysis of the treatments was undertaken by IEF/SDS-PAGE, comparison of protein spot abundances and

28 precolumn labeling and cannot be used for CA-IEF, at least without more detailed study of the dye-pro

29 g the three formulations: 60.1 mum/min in CA-IEF, 2.5 mum/min in IPG-IEF (~24-fold reduction versus C

30 min in IPG-IEF (~24-fold reduction versus CA-IEF), and 1.4 mum/min in mixed-bed IEF (~43-fold reducti

31 mixed-bed IEF (~43-fold reduction versus CA-IEF).

32 d comparable resolution to prominent on-chip IEF techniques.

33 ulteration markers to be assayed in combined IEF-immunoblotting procedures; the latter ones showing o

34 rn blotting protocols in solving the complex IEF pattern of casein (CN) mixtures observed when Italia

35 g experimental time compared to conventional IEF IGP gel strip approaches.

36 The improvement in PSNR was up to 2.34 dB, IEF improvement was more than 20%, and the improvement i

37 The first electrophoretic dimension (IEF OFFGEL) showed that dissolved proteins in surface se

38 reversed-phase HPLC in the second dimension (IEF-NP RP HPLC).

39 During IEF, the 2% PEG highly porous PA gel formulation offered

40 by 1D isoelectric focusing electrophoresis (IEF)-laser ablation (LA) inductively coupled plasma mass

41 sulfate polyacrylamide gel electrophoresis (IEF/SDS-PAGE) and fluorescence measurements.

42 ct") via an isoelectric focusing experiment (IEF).

43 n example, outlet fractions from cascaded FF-IEF were analyzed by sodium dodecyl sulfate polyacrylami

44 demonstrate the compatibility of cascaded FF-IEF with common bioanalytical tools.

45 abricated free-flow isoelectric focusing (FF-IEF) device.

46 we present a microfluidic free flow IEF (FF-IEF) device for continuous protein separation into 24 fr

47 demonstrate improved separation with the FF-IEF system over traditional 2D gel electrophoresis.

48 A giant internal electric field (IEF) in co-assembly structure was built by the larger lo

49 nd a strong interfacial electrostatic field (IEF of 10(9)~10(10) V/m).

50 496.0 V/cm) or Intermediate electric field - IEF (840.0 V/cm).

51 nal IEF, we present a microfluidic free flow IEF (FF-IEF) device for continuous protein separation in

52 c proteomic analysis of isoelectric focused (IEF) cerebrospinal fluid (CSF) immunoglobulin G (IgG) wa

53 um techniques such as iso-electric focusing (IEF) have become instrumental within analytical chemistr

54 h those obtained using Isoelectric focusing (IEF) and DNA tests.

55 y couples liquid-phase isoelectric focusing (IEF) and free solution capillary electrophoresis (CE).

56 -resolved microfluidic isoelectric focusing (IEF) and in situ antibody blotting IEF was employed to m

57 of T. pallidum by the isoelectric focusing (IEF) and nonequilibrating pH gel electrophoresis (NEPHGE

58 Microfluidic isoelectric focusing (IEF) can resolve proteoforms in lysate from low-to-singl

59 s based on concomitant isoelectric focusing (IEF) detection of B gamma2- and gamma3-CN fragments afte

60 of ultrafiltration and isoelectric focusing (IEF) electrophoresis steps.

61 m whole sporozoites by isoelectric focusing (IEF) following observations that the approximately 1,300

62 labeled proteins using isoelectric focusing (IEF) have been made.

63 ta (Abeta) peptides by isoelectric focusing (IEF) in 75 nL microchannels combined with their analysis

64 the resolving power of isoelectric focusing (IEF) in a polymeric microfluidic chip.

65 tigation of the use of isoelectric focusing (IEF) in microfluidic devices, pH gradients were electroc

66 to perform transverse isoelectric focusing (IEF) is presented as a means for continuous concentratio

67 Isoelectric focusing (IEF) is the first step for two-dimensional (2D) gel elec

68 we applied a capillary isoelectric focusing (IEF) method to determine the pattern of FOXO3 posttransl

69 After isoelectric focusing (IEF) of a methylated tryptic digest of a mixture of alph

70 Isoelectric focusing (IEF) of Cx43 in HuH-7 cells indicated that the pIs of th

71 ion polymorphism), and isoelectric focusing (IEF) of water-soluble proteins of fish fillet were appli

72 ter by two-dimensional isoelectric focusing (IEF) OFFGEL-lab-on-chip (LOC) electrophoresis after tang

73 After separation by isoelectric focusing (IEF) or non-equilibrium pH gradient electrophoresis (NEP

74 recognized by altered isoelectric focusing (IEF) patterns of serum transferrin (Tf).

75 Immunoprobed isoelectric focusing (IEF) resolves proteins based on differences in isoelectr

76 were then subjected to isoelectric focusing (IEF) separation by a digital ProteomeChip (dPC), followe

77 eta phosphorylation by isoelectric focusing (IEF) shows that LAP is present in multiple forms, each w

78 , and bla(OXA), and in isoelectric focusing (IEF) tests, all isolates demonstrated at least one beta-

79 platform that includes isoelectric focusing (IEF) through immobilized pH gradient and superficially p

80 inge has been used for isoelectric focusing (IEF) utilizing the stainless-steel needle and plunger as

81 Sub-zero isoelectric focusing (IEF) was employed to investigate the extent of hybrid fo

82 The new methods couple isoelectric focusing (IEF) with high ionic strength electrophoretic separation

83 , combining non-native isoelectric focusing (IEF) with sodium dodecyl sulfate (SDS) gel electrophores

84 By combining isoelectric focusing (IEF) with subsequent gel electrophoresis, two-dimensiona

85 By isoelectric focusing (IEF), 7 of the 23 isolates contained a beta-lactamase wi

86 Compared to isoelectric focusing (IEF), the CZE method provides more quantitative results

87 kers, electrophoresis, isoelectric focusing (IEF)-double immunoblotting, aptamer/antibody-based metho

88 l incompatibility with isoelectric focusing (IEF).

89 eneity was analyzed by isoelectric focusing (IEF).

90 mpared to conventional isoelectric focusing (IEF).

91 methods were used for isoelectric focusing (IEF).

92 ses by two-dimensional isoelectric focusing (IEF)/SDS-PAGE not only revealed different alpha and beta

93 a, acid urea PAGEs and isoelectric focusing (IEF)] as the first-dimension (1-DE).

94 f protein labeling with noncovalent dyes for IEF is investigated.

95 Both analytical and computational models for IEF suggest device performance will be improved by utili

96 desalting of samples, which is necessary for IEF, tends to aggregate halophilic proteins, often requi

97 e gel acts as an immobilization scaffold for IEF-focused proteins via photoactive moieties.

98 al feeding with an immune-enhancing formula (IEF) decreases morbidity, mortality, and length of hospi

99 in depletion, lectin affinity fractionation, IEF separation, and LC-MS analysis has been applied to d

100 tially sampling protein analytes eluted from IEF, focused proteins are electrokinetically transferred

101 as do several tryptic peptides produced from IEF SSP 3521 and chick p60).

102 FS-IEF-derived CRCL-pulsed DCs are a promising anticancer v

103 -solution isoelectric focusing technique (FS-IEF) to obtain chaperone-rich cell lysate (CRCL) fractio

104 genes (ISGs) and immune effector functions (IEFs).

105 ased technology (MGT), which combines in-gel IEF-LC-MS/MS and SDS-PAGE-LC-MS/MS strategies.

106 and quantitatively to conventional slab gel IEF.

107 urification (bulk soil --> crude colloid --> IEF colloid) and coincided with the trend of Pu concentr

108 However, IEF assays often use carrier ampholytes (CAs) to establi

109 ine WB milk/dairy products and comigrates in IEF with B gamma2-CN.

110 lectin column (M-LAC) and the pI profiles in IEF separation.

111 73x) of species were achieved in the initial IEF dimension.

112 ilized pH gradient isoelectric focusing (IPG-IEF) for the analysis of these apolipoproteins isolated

113 : 60.1 mum/min in CA-IEF, 2.5 mum/min in IPG-IEF (~24-fold reduction versus CA-IEF), and 1.4 mum/min

114 f simple, fast and cheap isoelectrofocusing (IEF) methods for evaluating the nature of the raw materi

115 Single and double isoelectrofocusing (IEF) of porcine sperm extracts generated fractions with

116 o integrate different separation mechanisms (IEF, CE, SEC, RPLC, HILIC, HIC, and IEX) are discussed c

117 entration of the eluate in the microchannel, IEF-micropillar-MALDI-MS is demonstrated to be a suitabl

118 resolution (0.1 pH unit) and rapid (<20 min) IEF.

119 ation formalism polarizable continuum model (IEF-PCM) at the HF/6-31+G(d) level.

120 Quantitative evaluations PSNR, MSE, IEF, SSIM, FOM and VIF clearly show the performance supe

121 The development of this novel IEF method for the simultaneous quantification of differ

122 ndard proteins demonstrates compatibility of IEF-SPLC processing and high resolving-power MS analysis

123 s also demonstrate that by the generation of IEF-SPLC protein libraries by fraction collection, the p

124 ognized by 3E2 in 2-D Western immunoblots of IEF-isolated CSL.

125 ies protein targets through immunoprobing of IEF-separated proteins that have been immobilized onto a

126 the array, thereby increasing the number of IEF fractions further analyzed in the size-based separat

127 nsional separation, including rehydration of IEF strip and fluorescence detection was completed in 2.

128 pare the microscale pH gradient stability of IEF established with IPGs, CAs, and a hybrid formulation

129 This method couples several stages of IEF in series by first focusing proteins in a straight c

130 Three stages of IEF were completed in less than 25 min at electric field

131 system described here facilitates the use of IEF markers for internal calibration of pI.

132 On IEF gels, only 4 to 20 bands were observed in the anti-S

133 ies include IgG2 dominance, limited bands on IEF gels, supporting an oligoclonal response, and use of

134 characterized the effect of gel pore size on IEF separation and in-gel immunoassay performance.

135 Our IEF results were consistent with theoretical pIs of meth

136 leavage at cysteine 245, focused on PhastGel IEF near pH 3.4, indicating the presence of three phosph

137 This work shows that a preparative IEF method using immobilized pH gradients can be optimiz

138 A variety of preparative IEF methods have been developed over the years but have

139 Therefore, preparative IEF methods are needed to fractionate charge variants fo

140 Our facile dynamic and probed IEF assays should find widespread use in analytical scre

141 o the transformation-sensitive human protein IEF SSP 3521 and mouse extendin.

142 In addition to purification, IEF preconcentration provides at least a 10-fold increas

143 toward Phe-pNA, together with other results (IEF) suggest the presence of more than one aminopeptidas

144 Most showed IEF profiles consistent with production of both a TEM an

145 to be applicable by using focusing of small IEF markers as a demonstration.

146 en fluorescent protein from the second-stage IEF fractionation were further separated in a third stag

147 Consequently, the strong IEF significantly activates PFAS molecules and reduces t

148 ere is more convenient than room-temperature IEF techniques, which require Hb mixtures in the deoxy s

149 with these symptoms and similar abnormal Tf IEF patterns were analyzed by metabolic labeling of fibr

150 It is demonstrated that IEF-NP RP HPLC provides a viable alternative to the 2-D

151 The IEF group received significantly more protein, carbohydr

152 The IEF profiles of the remaining 16 isolates showed a varie

153 V was applied for 5.0 min to accomplish the IEF and increased to -400 V during the infusion to ESI-M

154 ferent experimental parameters affecting the IEF process and the coupling of the IEF syringe with ele

155 g-cc-pVTZ and 6-31+G(d,p) basis sets and the IEF-polarizable continuum model (PCM) solvation model.

156 M06/def2-TZVPD levels of theory applying the IEF-PCM water and MeCN solvation models, all of which su

157 osis of histidinemia was demonstrated by the IEF syringe-ESI-MS system with accuracy from 88.25% to 1

158 To define the IEF axis along a "lane" at the top of the chamber, we us

159 During the IEF stage, the gel functions as an anti-convective mediu

160 YP/6-31+G(d,p) level of theory employing the IEF-PCM CHCl3 solvation model were also performed.

161 loric intake was 61% and 22% of goal for the IEF and CNTL groups, respectively.

162 in 70.0% (v/v) acetonitrile was used for the IEF of histidine.

163 used to repeatedly inject effluent from the IEF dimension into an ampholyte-based CE separation.

164 yzing all fluid volumes of interest from the IEF dimension, as IEF was 'parked' during each CE analys

165 ed the presence of HS functionalities in the IEF extract.

166 human erythroleukemia cell line lysate, the IEF-NP RP HPLC produced improved resolution of low mass

167 ry (ESI FTICR-MS) spectral comparison of the IEF extract and a siderophore standard (desferrioxamine;

168 t-stable proteins in the anodic range of the IEF gels.

169 f 20 microm/s during characterization of the IEF step.

170 ting the IEF process and the coupling of the IEF syringe with electrospray ionization mass spectromet

171 Mobilization drove focused proteins off the IEF lane and into a region for protein gel electrophores

172 y chemical mobilization perpendicular to the IEF axis.

173 tic activity was primarily attributed to the IEF between NDINH and PDINH, significantly accelerating

174 ed proteins increased significantly when the IEF fractionation step was included as part of the platf

175 the solubilisation buffer combined with the IEF approach.

176 d,p), and B97D/6-311+G(d,p) methods with the IEF-PCM solvation model for chloroform and ethanol.

177 stigated by using density functional theory (IEF-PCM + B3PW91-GD3/x2c-TZVPall).

178 Through IEF, each population was observed to exhibit distinct is

179 r underwent resection and were randomized to IEF via jejunostomy tube or control (CNTL).

180 of a fluid volume spanning 15% of the total IEF channel length was completed in less than 5 min.

181 dress some of the limitations of traditional IEF, we present a microfluidic free flow IEF (FF-IEF) de

182 Transverse IEF in pressure-driven flow is demonstrated using bovine

183 te the potential of "microfluidic transverse IEF" for use in continuous concentration and separation

184 g states in the solvent phase (DF/def2-TZVP; IEF-PCM and/or SMD) to investigate how well the experime

185 ential was the one obtained by OH when used, IEF, where the TPC was significantly higher than in the

186 Using IEF-NP RP HPLC, approximately 700 bands were resolved in

187 y of eIF4E-BP1 isoforms was determined using IEF/SDS/PAGE/immunoblotting of unfractionated cell lysat

188 There was one bowel necrosis associated with IEF requiring reoperation.

189 and band-broadening behavior consistent with IEF and CE, respectively.

190 the total analysis time, by implementing (x)IEF x (x)SEC x (t)RPLC separation stages.

191 the resulting peak capacity of a spatial (x)IEF x (x)SEC x (t)RPLC device.

192 The sub-zero IEF technique discussed here is more convenient than roo