ライフサイエンスコーパス: IgG2

1 dependent on the antibody subclass (IgG1 vs IgG2).

2 ncluding the lack of one glycine as found in IgG2.

3 maRIIb shows strongly increased affinity for IgG2.

4 ng residues being preserved between IgG1 and IgG2.

5 nd explain the receptor-binding functions of IgG2.

6 dification in a recombinant variant of human IgG2.

7 e IgG2), was reduced to levels equivalent to IgG2.

8 in HeLa cells interacted with human IgG1 and IgG2.

9 cells, and increased P. gingivalis-specific IgG2.

10 educed IFN-gamma- and P. gingivalis-specific IgG2.

11 promote both immunopathology and protective IgG2.

12 f IgA1 or IgA2m(1) but not in the context of IgG2.

13 and preferentially recognized either IgG1 or IgG2.

14 y, as, unlike huFcgammaRIIb, it avidly binds IgG2.

15 low anti-alpha-enolase/high anti-annexin AI IgG2.

16 ferent peptide backbones, derived from IgG1, IgG2/3, IgG4, IgA1, IgA2, and the joining chain from dim

17 Similarly, the recombinant anti-human CD14 IgG2/4 Ab, r18D11, was generated with greatly reduced Fc

18 rization of eculizumab, a hybrid therapeutic IgG2/4 mAb.

19 d the QTL that influences both total IgG and IgG2(a/b/c) Ab response to either Ad/E2TM or pE2TM.

20 antibodies in cell-based assays was: IgG1 > IgG2-A > IgG2 >> IgG2-B.

21 a subset showed activity differences between IgG2-A and IgG2-B.

22 IgG2-A is the known classic structure for the IgG2 subcl

23 ted with an increased hydrodynamic radius of IgG2-A relative to IgG2-B, as shown by biophysical chara

24 ns, the IgG2 disulfide isoforms converted to IgG2-A when 1 m guanidine was used, whereas IgG2-B was e

25 cells initially produce primarily one form (IgG2-A), which is rapidly converted to a second form (Ig

26 an ensemble of distinct isoforms, designated IgG2-A, -B, and -A/B, which differ by the disulfide conn

27 subclass, and we have named these structures IgG2-A, -B, and -A/B.

28 ain type, with IgG2lambda composed mostly of IgG2-A.

29 Multiple subvariants of the IgG2-A/B and IgG2-B structures are identified; these sub

30 IgG2-A/B represents an intermediate form, defined by an

31 which is rapidly converted to a second form (IgG2-A/B) while circulating in the blood, followed by a

32 significantly higher AFC, GC, and Th1-skewed IgG2 Ab (especially IgG2c) responses against the T cell-

33 Cs led to augmented Ab-forming cell, GC, and IgG2 Ab responses in Mer(-/-) mice, which were sustained

34 gG-producing Ab-forming cell, total IgG, and IgG2 Ab responses were also increased in Mer(-/-) mice.

35 ent study extended this correlation to human IgG2 Ab variants in primates.

36 ag proteins (e.g., p24) and/or production of IgG2 Abs against HIV-1 proteins.

37 onsistent with elevated levels of Th1-biased IgG2 Abs in Mer(-/-) mice.

38 of IgG2 from Ig preparations indicated that IgG2 Abs to HIV-1 p24 do not enhance phagocytosis, sugge

39 lymphocytic choriomeningitis virus-specific IgG2 Abs were dramatically decreased, whereas there was

40 In eRA, IgG2 against P. gingivalis was associated with ESR (P =

41 The observed distinction between IgG1 and IgG2 aggregation resulted from differential stability of

42 e immunization typically stimulated IgG1 and IgG2, AIT is often associated with production of IgG4.

43 ency of genotypes containing the low-binding IgG2 allele, FcgammaRIIa-R131, was significantly greater

44 IgG1 comprised 50-80% of the repertoire, and IgG2 alleles comprised < 10% in nearly all tissues.

45 In contrast, bovine IgG2 and human IgG1 antibodies were cleaved rapidly into

46 By showing stronger virus-specific IgG2 and IgA responses in patients with Nef-deficient vi

47 was associated with elevated serum levels of IgG2 and IgG3 anti-dsDNA antibodies and accumulation of

48 The response involved predominantly IgG2 and IgG3 antibody subclasses.

49 ity of IgG1 during its normal life span; for IgG2 and IgG3 the inter-heavy chain disulfide bonds are

50 allograft and with production of anti-LIMS1 IgG2 and IgG3.

51 both species, fundamental differences in the IgG2 and IgG4 Ab subclasses were found between the two s

52 In humans, IgG2 and IgG4 adapted a silent Fc region with weak bindi

53 IgG2 and IgG4 anti-GXM prolonged the lives of infected B

54 and IgG3, respectively, but no increase with IgG2 and IgG4 anti-RhD Abs.

55 ons, whereas, in contrast, cynomolgus monkey IgG2 and IgG4 display strong effector function as well a

56 IgG2 and IgG4 isotypes have significantly lower binding

57 ings, in vitro and in vivo results for human IgG2 and IgG4 obtained in the cynomolgus monkey have to

58 the gamma heavy chain was restricted to the IgG2 and IgG4 subclasses, suggesting the binding of mono

59 dies of many different IgG subclasses (IgG1, IgG2 and IgG4) are used in the treatment of various canc

60 subclass, has a half-life as long as that of IgG2 and IgG4, binds the FcgammaR receptor, and activate

61 nt and these EC antibodies were enriched for IgG2 and IgG4, noncomplement activating subclasses.

62 etermination showed AECAs to be enriched for IgG2 and IgG4, subclasses that do not activate complemen

63 uce heterogeneous disulfide bonding in human IgG2 and maintain in vitro activity.

64 diated transcytosis of V(H)-matched IgG1 and IgG2 and mutated variants thereof lacking Fc-gamma recep

65 high anti-alpha-enolase/low anti-annexin AI IgG2 and patients with low anti-alpha-enolase/high anti-

66 y and the levels of S. mansoni worm IgG1 and IgG2 and Plasmodium falciparum IgG1 and IgG4.

67 Bi-trait variance component analyses of IgG2 and quantitative measures of AgP indicate that diff

68 provided data suggesting a potential role of IgG2 and specific AM oligosaccharides.

69 IgG antibodies to PEG-uricase, mostly IgG2 and specific for PEG, developed in 9 patients, who

70 evels of serum anti-alpha-enolase (>15 mg/L) IgG2 and/or anti-annexin AI (>2.7 mg/L) IgG2 were detect

71 Conduits attenuated immunoglobulin G2 (IgG2) and IgA class switching in systemic and intestinal

72 P are recognized by serum immunoglobulin G2 (IgG2) and stimulate memory T-lymphocyte proliferation an

73 immunoglobulin (Ig) G, its subtypes IgG1 and IgG2, and IgA and IgM in vaccinated animals.

74 markable IgG responses that are dominated by IgG2, and IgG2 is IFN-gamma-dependent and is promoted by

75 es had elevated levels of Env-specific IgG1, IgG2, and IgG3 Abs compared with males.

76 ly useful properties compared with the IgG1, IgG2, and IgG3 subclasses.

77 Immunoglobulin G subclasses IgG1, IgG2, and IgG4 samples were first stressed in protonated

78 rationalize the existence of the human IgG1, IgG2, and IgG4 subclasses and explain the receptor-bindi

79 ) mice had improved humoral responses (IgG1, IgG2, and IgM), a result that further explains the strai

80 e that express human immunoglobulin M [IgM], IgG2, and kappa) which were immunized with a C. neoforma

81 in a solution of human monoclonal antibody, IgG2, and the effects of human serum albumin, a major bl

82 nd Porphyromonas gingivalis are dominated by IgG2, and these IgG2 responses are associated with reduc

83 B cells into PCs, induced class switching to IgG2, and was reproducible in cocultures with neutrophil

84 ranulocytes, the main effector cells against IgG2- and IgG4-opsonized bacteria and parasites, do not

85 Anti-DNA IgG3 was the unique non-IgG2 anti-DNA deposit, and anti-C1q IgG4 was mainly dete

86 es correlated with plasma levels of IgG1 and IgG2 anti-HIV-1 p24 and, notably, correlated inversely w

87 ssue of the JCI that recognition of IgG1 and IgG2 antibodies by FcgammaRIII and FcgammaRIV receptors

88 All IgG2 antibodies displayed the same disulfide conversion,

89 uctural isoforms are present in native human IgG2 antibodies isolated from myeloma plasma and from no

90 We found that IgG2 antibodies predominated in the response to vaccinat

91 However, with the exception of IgG2 antibodies to gp41, HLA status was not associated w

92 d-induced aggregation of monoclonal IgG1 and IgG2 antibodies was studied at pH 3.5 as a function of s

93 Human monoclonal IgG1 and IgG2 antibodies were designed with identical antigen bin

94 A pharmacokinetics study of two mutant IgG2 antibodies with increased FcRn binding affinity ind

95 both human immunoglobulin gamma 1 (IgG1) and IgG2 antibodies.

96 due in the C(H)1 constant domain of IgG1 and IgG2 antibodies.

97 d by excess production of O-antigen-specific IgG2 antibodies.

98 uctural variants of human immunoglobulin G2 (IgG2) antibodies was recently the subject of two copubli

99 tolerability of AMG 145, a human monoclonal IgG2 antibody against PCSK9, in stable patients with hyp

100 o additional monoclonal antibody species: an IgG2 antibody and an IgG1 antibody conjugate.

101 Human IgG2 antibody displays distinct therapeutically-useful p

102 nstrates that the disulfide structure of the IgG2 antibody is dynamic in vivo, on a time scale simila

103 in skin test strongly boosted IgG, IgG1, and IgG2 antibody responses, particularly against MPB83 and

104 Recently, the human IgG2 antibody subclass was found to possess multiple str

105 Denosumab is a fully human monoclonal IgG2 antibody that binds RANKL, inhibiting its activity.

106 used to separate populations of recombinant IgG2 antibody that were created as a result of prolonged

107 Applying our approach to a stressed IgG2 antibody, 10 cross-linked peptides were discovered

108 ully human monoclonal immunoglobulin gamma2 (IgG2) antibody panitumumab against human epidermal growt

109 interferon secretion and immunoglobulin G2 (IgG2) antibody titers.

110 how a multiantibody composition in LN, where IgG2 autoantibodies against alpha-enolase and annexin AI

111 Notably, IgG2 autoantibodies against alpha-enolase and annexin AI

112 IgG2 autoantibodies against DNA, histones (H2A, H3, and

113 Anti-H3, anti-DNA, and anti-C1q IgG2 autoantibodies were also prevalent in LN serum, whi

114 e, the connectivity of a novel subvariant of IgG2-B containing an intrachain disulfide linkage in the

115 IgG2-B is a structure defined by a symmetrical arrangeme

116 Multiple subvariants of the IgG2-A/B and IgG2-B structures are identified; these subvariants of e

117 IgG2-A when 1 m guanidine was used, whereas IgG2-B was enriched in the absence of guanidine.

118 owed by a slower conversion to a third form (IgG2-B).

119 ed hydrodynamic radius of IgG2-A relative to IgG2-B, as shown by biophysical characterization.

120 owed activity differences between IgG2-A and IgG2-B.

121 ll-based assays was: IgG1 > IgG2-A > IgG2 >> IgG2-B.

122 e quality of the immune response (i.e., IgG1/IgG2 balance and mucosal IgA and IL-17 secretion) was de

123 ll, our results highlight KTN0073 as a novel IgG2-based MET mAb that acts through exon 14-independent

124 ensity malaria, whereas individuals with the IgG2-binding Fc gamma RIIa-His/His131 genotype are at in

125 The present study suggests that the IgG2-binding Fc gamma RIIa-His/His131 genotype is associ

126 and complexes (PEG-GCSF; an IgG1k; IgG1- and IgG2-biotin covalent conjugates; the membrane protein co

127 n IgG1 antibody and a Cys-232 --> Ser mutant IgG2, both of which are homogeneous with respect to disu

128 at the lower binding of CD32A(R) not only to IgG2 but also to IgG1 and IgG3 might be responsible for

129 A novel FcgammaR that binds IgG2 but not IgG1 has just been identified, potentially

130 on of IFN-gamma also restored IND-suppressed IgG2 but not IgG1.

131 s as the glomerular deposits (mostly IgG1 or IgG2), but only one patient had myeloma.

132 ignificantly lower binding not only to human IgG2, but also to IgG1 and IgG3 subtypes.

133 ined the solution structure of human myeloma IgG2 by atomistic X-ray and neutron-scattering modeling.

134 all the disulfide bridges and show that the IgG2 C H1 and C-terminal C L cysteine residues are eithe

135 Furthermore, nephritogenic monoclonal IgG2 (clone H147) derived from lupus-prone MRL-lpr/lpr m

136 4, both of which are present in the IgG1 and IgG2 constant domain sequences, and Asn-35, which was pr

137 Grafting the mAb variable regions onto the IgG2 constant region dramatically enhanced the tumor inh

138 robably due to the enhanced stability of the IgG2 construct, than previously observed with (131)I-mur

139 The hinge region of human IgG2 contains four cysteine residues involved in disulfi

140 asurable levels of D-cysteines were found on IgG2 cysteines in the hinge region, both with monoclonal

141 ss IV nephritis and in patients with intense IgG2 deposition.

142 IgG2 dimer formation was <=5% and independent of the buf

143 The potential role of IgG2 dimers in immunity against carbohydrate Ags is disc

144 IgG1 and IgG2 have similar half-lives, yet IgG2 displays lower foetal than maternal concentration a

145 The complication of IgG2 disulfide connections demands advances in technique

146 crystal structure analysis, we propose that IgG2 disulfide exchange is caused by the close proximity

147 structural and functional properties of the IgG2 disulfide isoforms and compared them to IgG1.

148 Under reduction-oxidation conditions, the IgG2 disulfide isoforms converted to IgG2-A when 1 m gua

149 the fundamental framework of three distinct IgG2 disulfide isoforms recently described.

150 -containing disulfide peptides produced from IgG2 disulfide isoforms.

151 Here we show that these IgG2 disulfide linkages interconvert while circulating i

152 in this paper reveal that the population of IgG2 disulfide structural variants is yet more complex t

153 Thus, changes to the IgG2 disulfide structure provide a marker of the protein

154 Human IgG2 exists as a mixture of disulfide-linked structural

155 No reactivity against IdeS-generated IgG2-F(ab')2s was detected.

156 c glycan profiles and minimal differences in IgG2 Fc glycans were noted, whereas the presence or abse

157 fied Ig binding, with a preference for human IgG2 Fc, and localized the IgG-binding region to a highl

158 tion of TFSS antigens by CD4+ T cells and by IgG2 from cattle immunized with the protective outer mem

159 reatest in HIV controllers, and depletion of IgG2 from Ig preparations indicated that IgG2 Abs to HIV

160 ce) and expressing either immunoglobulin G2 (IgG2) (G2 mice) or IgG4 (G4 mice).

161 t region (G1)) or without effector function (IgG2/G4 fusion constant region (G2G4)) exhibited high an

162 s provides strong evidence that the observed IgG2 gas-phase conformers are related to disulfide bond

163 Conversely, transport of IgG2 + Gly236 was increased to IgG1 levels.

164 2 doses of PsA-TT had the greatest IgG1 and IgG2 GMCs of 125.23 microg/mL and 36.12 microg/mL, respe

165 Group A-specific IgG1 and IgG2 GMCs remained greater in the PsA-TT group than in t

166 es in cell-based assays was: IgG1 > IgG2-A > IgG2 >> IgG2-B.

167 unlike other immunoglobulin isotypes, human IgG2 (h2) imparts FcgammaR-independent agonistic activit

168 Anti-Hcp1 IgG1 and anti-OPS IgG2 had the greatest AUROCCs (0.87 and 0.95, respective

169 d serum-mediated killing of P. aeruginosa by IgG2 have poorer respiratory function than infected pati

170 IgG1 and IgG2 have similar half-lives, yet IgG2 displays lower fo

171 istamine, antibody activity (IgG, IgE, IgG1, IgG2, IgA), cytokines (IL-4, IFN-gamma, IL-12p70, IL-10,

172 gG1 sequence with those of other subclasses (IgG2, IgG3 and IgG4) showed that these aggregation-prone

173 comparable levels of rMal d 1-specific IgG1, IgG2, IgG3, and IgG4 antibodies.

174 shows distinct properties compared with the IgG2, IgG3, and IgG4 subclasses and is the most exploite

175 DNA technology, 5C12 isotype variants (IgG1, IgG2, IgG3, and IgG4) and antibody fragments [Fab, F(ab'

176 dopeptidase activity were obtained for IgG1, IgG2, IgG3, and IgG4.

177 tigen (SWA)-specific immunoglobulin (Ig) G1, IgG2, IgG3, IgG4, interleukin (IL)-4, and IL-5 increased

178 uals were rarely positive for anti-chlamydia IgG2, IgG4 or IgA2.

179 Therefore, an inert human IgG2/IgG4 hybrid C region was chosen for an rMil2.

180 ody-related products is increasing including IgG2/IgG4 subclasses and engineered Fc regions to enhanc

181 f the feline heavy chains of IgG1a (IGHG1a), IgG2 (IGHG2), and IgA (IGHA), and the light chains (lamb

182 on of the inflammatory factors such as IgG1, IgG2, IgM, IL-6 and PMPhi phagocytosis, stimulation of s

183 vity similar to that of the negative control IgG2 in a CD20(+) human Raji lymphoma cell line.

184 creased activity of the IgG1 relative to the IgG2 in blocking interleukin-1beta ligand from binding t

185 lization of alpha-enolase or annexin AI with IgG2 in glomeruli.

186 is, and proteins recognized by total IgG and IgG2 in immune sera of outer membrane-vaccinated cattle

187 subjects, likely because of the presence of IgG2 in the complexes.

188 etric mean concentrations (GMCs) of IgG1 and IgG2 in the PsA-TT group were 21.73 microg/mL and 6.27 m

189 F) from LagP patients and the high levels of IgG2 in their serum and GCF that is reactive with A. act

190 gG responses that are dominated by IgG2, and IgG2 is IFN-gamma-dependent and is promoted by dendritic

191 Structural information on IgG2 is limited by the absence of a full-length crystal

192 nalytical ultracentrifugation disclosed that IgG2 is monomeric with a sedimentation coefficient (s (2

193 IgG2 is the second most abundant IgG subclass, being abl

194 to the receptor, suggesting that some of the IgG2 isoforms had lower activity.

195 Furthermore, the IgG2 isoforms were shown to interconvert in whole blood

196 Recent evidence suggests that the IgG2 isotype is not completely devoid of effector functi

197 mation by interacting with IgG1, even though IgG2 isotypes tend to be more pathogenic.

198 cyte responses and specific Abs dominated by IgG2 isotypes.

199 icate that different genes appear to control IgG2 levels and disease susceptibility.

200 Anti-H3 and anti-alpha-enolase IgG2 levels had the most remarkable increase in LN serum

201 cytokine levels in CD4 T cells and increased IgG2 levels in sera.

202 l phenotype (generating 2.5-fold higher IgG1/IgG2 levels).

203 mplishes the reduced tryptic digestion of an IgG2 mAb in a mildly acidic condition (pH 6.0) with half

204 d in the separation of disulfide isoforms of IgG2 mAbs by CZE.

205 her than the respective IgG1 MAbs, while the IgG2 MAbs had the least activity.

206 gy to produce fully human immunoglobulin G2 (IgG2) MAbs from B cells of an individual post-Staphyloco

207 gG1 decreased activity, whereas switching to IgG2 markedly increased activity.

208 uggesting that the in vivo activity of human IgG2 may be dependent on the distribution of isoforms.

209 DEK autoantibodies (IgG2) may activate the complement cascade, primarily rec

210 The IgG1:IgG2 mean ratio decreased following successive vaccinati

211 demonstrated a significant increase in IgG1:IgG2 mean ratio, indicative of the T-cell-dependent resp

212 ized AgP-affected members (274 subjects with IgG2 measurements).

213 tween different Gram-negative bacteria, this IgG2-mediated impairment of killing may operate in other

214 ently reported to take place in serum for an IgG2 molecule and resulted in predictable mature isoform

215 V(L) domain sequence of a recombinant human IgG2 molecule.

216 IgG2 molecules are organized differently from that model

217 t studies of the covalent structure of human IgG2 molecules.

218 present the detailed disulfide structure of IgG2 molecules.

219 reproducible separation of multiple IgG1 and IgG2 monoclonal antibodies (mAbs) was obtained with a lo

220 activity studies were extended to additional IgG2 monoclonal antibodies with various antigen targets.

221 acterization of disulfide variants in intact IgG2 monoclonal antibodies.

222 ailed analysis showed that recombinant human IgG2 monoclonal antibody could be partially resolved int

223 Figitumumab is a fully human IgG2 monoclonal antibody targeting the insulin-like grow

224 Nonenzymatic glycation of an IgG2 monoclonal antibody was studied using affinity chro

225 /MS/MS analysis of a tryptic digestion of an IgG2 monoclonal antibody, 1712 peptide ions were identif

226 h FcgammaRIIa 131H is known to bind IgG1 and IgG2 more avidly, no such differences in affinity are kn

227 rom animal experiments have shown that human IgG2/mouse chimeric antitenascin 81C6 (ch81C6) monoclona

228 Following mutagenesis, several IgG2 mutants with increased binding affinity to human Fc

229 Here we investigated to what extent human IgG2 N-terminal glutamate converts to pE in vivo.

230 Interestingly, we also found that IgG2, naturally only binding FcgammaRIIa, gains binding

231 compared with a wild-type IgG1 (IgG1/wt) or IgG2 of identical antigen specificity.

232 ino acid substitutions from the Fc region of IgG2 or IgG4 antibodies, reduced but did not eliminate D

233 er monocyte infiltration into the graft than IgG2 or IgG4 due to enhancement by FcgammaR interactions

234 ng domains were each paired with human IgG1, IgG2, or IgG3.

235 cacy superior to that observed with IgG1/wt, IgG2, or IgG4 of identical antigen specificity.

236 ing of HIV-1 by neutralizing mAb b12 and CD4-IgG2 (PRO-542) blocked both localized infection and vira

237 Indomethacin treatment inhibited IgG1 and IgG2 production, and PGE2 restored both immunoglobulins

238 ocyte-derived dendritic cells (mDCs) promote IgG2 production.

239 n (IFN)-gamma (a Th-1 mediator) both promote IgG2 production.

240 ved greater persistence and higher titers of IgG2 protective antibodies.

241 The IgG2 proved to have similar modifications to Infliximab

242 osis and accounts for the low maternal/fetal IgG2 ratio at term.

243 Immune responses are dominated by IgG2 reactive with bacterial surface carbohydrates.

244 fluid and high titers of IFN-gamma-dependent IgG2 reactive with P. gingivalis in gingival crevicular

245 Anti-alpha-enolase IgG2 recognized specific epitopes of alpha-enolase and d

246 e characterized a series of Cys-->Ser mutant IgG2 recombinant monoclonal antibodies, focused on the f

247 ed mice had less histamine and IgG1 and more IgG2-related antibodies indicating a bias toward the typ

248 saline and isotype controls (human and mouse IgG2) remained unchanged.

249 ts develop a substantial anti-gp120-specific IgG2 response.

250 gingivalis are dominated by IgG2, and these IgG2 responses are associated with reduced extent and se

251 Anti-P. gingivalis IgG2 responses were enhanced by dendritic cells, and rem

252 N-gamma production and help explain elevated IgG2 responses.

253 and that this IFN-gamma selectively promotes IgG2 responses.

254 Human immunoglobulin G2 (IgG2) responses are gamma interferon (IFN-gamma) depende

255 han the individual fragments, similar to the IgG2 results.

256 However, IgG2 samples displayed a wide range of affinities, indic

257 ls of immunoglobulin M (IgM), IgG, IgG1, and IgG2 serum antibodies against ruminant C. abortus in a c

258 These results show that IgG2 structure is significantly different from the conve

259 Our IgG2 structures rationalize the existence of the human I

260 d in the determination of symmetric Y-shaped IgG2 structures.

261 gG2-A is the known classic structure for the IgG2 subclass defined by structurally independent Fab do

262 ng report, we have identified that the human IgG2 subclass exists as an ensemble of distinct isoforms

263 Little is known about regulation of the IgG2 subclass, although prostaglandin E2 (PGE2) (a media

264 ee main types of structures within the human IgG2 subclass, and we have named these structures IgG2-A

265 e presently accepted structure for the human IgG2 subclass, we also found major structures that diffe

266 sity to aggregate compared with those of the IgG2 subclass.

267 ident in a recombinant human antibody of the IgG2 subclass.

268 of a therapeutic monoclonal antibody of the IgG2 subclass.

269 pper hinge cysteine residues specific to the IgG2 subclass.

270 feature of antibodies belonging to the human IgG2 subclass.

271 ffect of endogenous IgG, especially from the IgG2 subclass.

272 eriments that utilized molecules of IgG1 and IgG2 subclasses with varying levels of C(H)2 glycosylati

273 as unchanged, with bias towards the IgG1 and IgG2 subclasses.

274 We have engineered both human IgG1 and IgG2 subtypes, with minimal point mutations, to form ful

275 ation with PsACWY, indicating a shift toward IgG2, suggestive of the T-cell-independent immune respon

276 for transport and that FcRn transported less IgG2 than IgG1.

277 and II clinical trials with AMG-162, a human IgG2 that binds receptor activator of nuclear factor kap

278 ents, and these patients have high levels of IgG2 that is reactive with Actinobacillus actinomycetemc

279 NAb response correlated with virus-specific IgG2 titers and that the in vivo neutralization potency

280 Depletion of IgG2 to O-antigen restores the ability of sera to kill s

281 We suggest that excessive binding of IgG2 to O-antigen shields the bacterium from other antib

282 ficient to explain the reduced FcRn-mediated IgG2 transcytosis and accounts for the low maternal/feta

283 For IgG2, two pepsin cleavage sites were identified; anti-hi

284 for proteins and its possible origins in the IgG2 variant are discussed.

285 In pre-RA, P. gingivalis-specific IgG2 was associated with ACPAs (P = 0.049) and disease s

286 Only 16% of the variance in IgG2 was attributable to age, race, and smoking.

287 In in vitro killing assays, anti-ChoP IgG2 was effective against some clinical isolates of non

288 IgG2 was the predominant anti-PEG IgG subclass.

289 P-specific human antibodies (mainly IgG1 and IgG2) was detected in all immunized mice.

290 Isotype diversification (toward IgG2) was greatest in HIV controllers, and depletion of

291 lower hinge (DeltaGly236, absent in germline IgG2), was reduced to levels equivalent to IgG2.

292 P. gingivalis IgG1 and IgG2 were analyzed.

293 contrast, AHA levels against pepsin-cleaved IgG2 were comparable.

294 g/L) IgG2 and/or anti-annexin AI (>2.7 mg/L) IgG2 were detected in most patients with LN but not pati

295 strong increase in IgG4 and some increase in IgG2 were observed throughout the study, while productio

296 occal capsular polysaccharide (total IgG and IgG2) were assessed at baseline and 16 wk.

297 eta, and IL-23, is particularly dependent on IgG2, whereas type I IFN responses are controlled by IgG

298 HH platelets were also sensitive to IgG2, which in contrast, failed to inhibit the response

299 reduce disulfide bond heterogeneity in human IgG2 while preserving the activity of this therapeutical

300 re, the isoforms are present in native human IgG2 with either kappa or lambda light chains, although