ライフサイエンスコーパス: KCC1

1 KCC1 adopts an inward-facing conformation, with the extr

2 KCC1 encodes a 1085-residue polypeptide with substantial

3 KCC1 exists as a dimer, with both extracellular and tran

4 KCC1 was identified by searching the human expressed seq

5 KCC1-specific antibody identified a 155-kDa protein in r

6 ion-polymerase chain reaction revealed a 2:1 KCC1/KCC3 mRNA ratio in VSMCs.

7 buted to potassium chloride cotransporter 1 (KCC1).

8 Although KCC1 has not been cloned from the colon, we established,

9 increase of colonic KCC1 mRNA abundance and KCC1 protein expression in potassium depletion of the ra

10 rythroblasts, a similar pattern for KCC3 and KCC1 expression during in vivo differentiation was obser

11 hreonine kinases, colocalizes with NKCC1 and KCC1/2 in diverse Cl(-)-transporting epithelia and in ne

12 inomycin D and cycloheximide increased basal KCC1 mRNA in an additive manner, suggesting different me

13 including AE3, AQP4, AQP5, CFTR, ClC2gamma, KCC1, NHE1, NKAalpha1, NKAbeta1, NKAbeta2, NKAbeta3, and

14 The increase of colonic KCC1 mRNA abundance and KCC1 protein expression in potas

15 es of human potassium-chloride cotransporter KCC1 in potassium chloride or sodium chloride at 2.9- to

16 rter NKCC1 and the K(+)/Cl(-) cotransporters KCC1, KCC2, KCC3 and KCC4 have deepened our understandin

17 C1 and Cl(-) efflux via K-Cl cotransporters, KCC1 or KCC2.

18 Message levels for KCC1 and KCC3b were low.

19 Human KCC1 (hKCC1) was obtained from I.M.A.G.E. clones and in

20 ibited dominant negative inhibition of human KCC1 and KCC3 and, with lower potency, mouse KCC4 and ra

21 understanding of the regulation of the human KCC1 gene, we mapped the 5' end of the KCC1 cDNA, cloned

22 ime- and concentration-dependent increase in KCC1 mRNA levels after treatment with sodium nitroprussi

23 sodium depletion resulted in an increase in KCC1 protein expression in basolateral membrane.

24 he NO/cGMP-signaling pathway participates in KCC1 mRNA regulation at the post-transcriptional level.

25 one potassium site and two chloride sites in KCC1, which are all required for the ion transport activ

26 Moreover, 8-Br-cGMP increased KCC1 mRNA expression in a concentration- and time-depend

27 dely distributed K-Cl cotransporter isoform (KCC1) identified in rat brain and rabbit kidney but only

28 that VSMCs express mainly two mRNA isoforms, KCC1 and KCC3, and suggest that PKG participates post-tr

29 ast two mRNAs for K-Cl cotransporters (KCC): KCC1 and KCC3.

30 rom the rat distal colon using rabbit kidney KCC1 cDNA as a probe, the presence of an expected size m

31 membrane H,K-ATPase and basolateral membrane KCC1 protein.

32 and C-terminal cytoplasmic domains of mouse KCC1 to its K-Cl cotransport function expressed in Xenop

33 Expression of KCC mRNAs (KCC1-KCC4) in rat vascular smooth muscle cells (VSMCs) i

34 were 100% conserved between human and murine KCC1 genes.

35 otransporters KCC3, NKCC1, and NKCC2 but not KCC1 and KCC4.

36 Hydropathy analysis of KCC1 indicates structural homology to NKCC, including 12

37 l as confirming the widespread expression of KCC1 and KCC2 throughout the brain, we now show a more r

38 in erythrocytes, with variable expression of KCC1 and KCC4 among individuals that could result in mod

39 /cGMP signaling pathway in the expression of KCC1 mRNA, considered to be the major cell volume regula

40 We have reported the presence of KCC1 and KCC3 mRNAs in primary cultures of VSMCs by spec

41 sGC-dependent regulation of KCC1 mRNA expression was confirmed using YC-1, a NO-inde

42 expression of the K-Cl cotransport protein, KCC1.

43 Rabbit KCC1 (rbKCC1) and rat KCC1 (rtKCC1) were cloned by scree

44 unctional comparison of mouse KCC3 to rabbit KCC1 indicates that KCC3 has a much greater volume sensi

45 Rabbit KCC1 (rbKCC1) and rat KCC1 (rtKCC1) were cloned by screening rabbit kidney and

46 tern blot analysis of RBC membranes revealed KCC1, KCC3, and KCC4 proteins in mouse and human cells,

47 tase-polymerase chain reaction revealed that KCC1 was present in rat primary astrocytes and rat C6 gl

48 We show that KCC1 intriguingly exists in one of two distinct dimeric

49 The KCC1 promoter was transactivated by forced expression of

50 The KCC1 structures allow us to model a potential ion transp

51 is elevated in sickle erythrocytes, and the KCC1 gene has been proposed as a modifier gene in sickle

52 etanide and ethacrynic acid, which block the KCC1 potassium-chloride transporter in the kidney loop o

53 orresponding genomic DNA, and identified the KCC1 gene promoter.

54 human KCC1 gene, we mapped the 5' end of the KCC1 cDNA, cloned the corresponding genomic DNA, and ide

55 -open CCC structure reveals that opening the KCC1 extracellular ion permeation path does not involve

56 These data demonstrate that the KCC1 cDNAs encode a widely expressed K-Cl cotransporter

57 This KCC1 mRNA is substantially increased by potassium deplet

58 CC1, and that it inhibits Cl(-) exit through KCC1 and KCC2; kinase-inactive WNK3 has the opposite eff

59 to the previously characterized transporters KCC1 and KCC2, with which they share a predicted membran

60 d its co-expression did not reduce wild type KCC1 at the oocyte surface.

61 117 was co-immunoprecipitated with wild type KCC1 polypeptide, and its co-expression did not reduce w

62 basolateral membrane of duct cells, whereas KCC1, IK(Ca)1, CFTR, and ClC3 are apically localized.

63 CC3a RNA was expressed consistently, whereas KCC1 and KCC3b levels declined, and KCC4 message first i