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1 L. amazonensis amastigotes, through activation of extrac
2 L. amazonensis is endemic in South America, where it cau
3 L. amazonensis metacyclic promastigotes lacking one SODA
4 Inhibition of arginase I or ODC abrogates L. amazonensis replication in infected human macrophages
11 compound 4 exhibited better results against L. amazonensis promastigotes (IC(50) = 15.52 3.782 uM) a
15 ation of virulent forms in both L. major and L. amazonensis Our results also uncover a unique require
17 ALB/c mice to infection with L. mexicana and L. amazonensis, does play a significant role in regulati
18 ed by the New World species, L. mexicana and L. amazonensis, we analyzed their course of infection in
19 plex parasites (L. mexicana, L. pifanoi, and L. amazonensis), a critical role for immunoglobulin G-me
21 subset of phagocytic B (termed B-1P) cells, L. amazonensis survives and proliferates at high rates i
22 that the CD4+ T-cell response during chronic L. amazonensis infection is limited during the transitio
23 c Th1 transfer helped recipient mice control L. amazonensis infection established by promastigotes bu
24 particular, neutrophil-derived LTB4 controls L. amazonensis killing, degranulation, and reactive oxyg
26 All phenotypes observed in LHR1/Deltalhr1 L. amazonensis were rescued by expression of episomal LH
28 of the Dectin-1/Syk/ROS/NLRP3 pathway during L. amazonensis phagocytosis is important for macrophage
29 We identified that ROS production during L. amazonensis infection occurs upon engagement of Decti
30 in regulating local immune responses during L. amazonensis infection, providing new insights into im
32 crophages from 5-LO knockout mice eliminated L. amazonensis in the presence of exogenous LTB4, and ma
35 es revealed that Retro-2 curbed experimental L. amazonensis infections in a dose-dependent manner.
36 e were injected locally with IP-10 following L. amazonensis infection, there was a significant delay
37 ls, as local injection of IL-1beta following L. amazonensis infection accelerated Th cell activation
38 n and interleukin-12p40 production following L. amazonensis amastigote infection compared with non-tr
41 the importance of efficient heme uptake for L. amazonensis replication and vertebrate host infectivi
42 comparison of draining lymph node cells from L. amazonensis- and L. major-infected mice at 10 weeks p
43 Antigen stimulation of CD4+ T cells from L. amazonensis-infected mice in vitro in the presence of
46 hmania amazonensis Our inability to generate L. amazonensis SODA null mutants and the lethal phenotyp
48 nal antibodies (MoAbs) against a non-healing L. amazonensis infection in BALB/c mice, and that anti-P
52 was reported for L. major, APX depletion in L. amazonensis enhanced differentiation of metacyclic pr
53 ization and membrane association of FCaBP in L. amazonensis suggest that the mechanisms for flagellar
55 hondrial alterations and lipid inclusions in L. amazonensis promastigotes, upregulated tumor necrosis
57 responsible for the susceptible phenotype in L. amazonensis-infected hosts and that this parasite may
60 ver, the adoptive transfer of ANK cells into L. amazonensis-infected mice markedly increased DC and T
65 In phagocytic cells such as macrophages, L. amazonensis modulates endosomal/lysosomal trafficking
66 head challenge infection with 107 metacyclic L. amazonensis promastigotes at 4 wk demonstrated protec
67 d amastigotes in IFN-gamma-stimulated Mphis, L. amazonensis-specific Th1 transfer helped recipient mi
68 fection established by promastigotes but not L. amazonensis infection established by amastigotes.
70 , we identify and characterize LIT1, a novel L. amazonensis membrane protein with extensive similarit
71 f interleukin-10 (IL-10), in CD4(+) cells of L. amazonensis-infected mice, we further examined whethe
74 lation of the macrophage-mediated killing of L. amazonensis in vitro and a less robust antibody respo
75 e ear with 10(5) metacyclic promastigotes of L. amazonensis together with SGE (equivalent to 0.5 glan
78 via NADPH oxidase during the early stages of L. amazonensis infection is critical for inflammasome ac
82 ent mice infected with either L. mexicana or L. amazonensis failed to control the lesion progression,
83 rprised to find that IFN-gamma could promote L. amazonensis amastigote replication in macrophages (Mp
87 iginal sites of inoculation, indicating that L. amazonensis can persist in vivo independently of the
93 ults reveal a quite unexpected aspect of the L. amazonensis parasite and have important implications
94 ential of a DNA-based vaccine, we tested the L. amazonensis gene encoding P4 nuclease as well as adju
99 ngly, inflammasome activation in response to L. amazonensis is impaired by inhibitors of NADPH oxidas
101 because IL-4-/- mice remained susceptible to L. amazonensis infection, even after IL-12 administratio
103 se mechanism of pathogenesis associated with L. amazonensis infection remains largely unresolved.
104 of bone marrow-derived dendritic cells with L. amazonensis amastigotes resulted in rapid and signifi
107 infection of C3HeB/FeJ mice for 7 days with L. amazonensis promoted an immature CD11c(+) DC phenotyp
108 between MHC class II -/- mice infected with L. amazonensis and Leishmania major suggest that these p
109 e response of mice chronically infected with L. amazonensis can be enhanced towards a Th1 phenotype b
111 functions at early stages of infection with L. amazonensis parasites and provide a compelling ration
114 d dendritic cells (DCs) of C57BL/6 mice with L. amazonensis or Leishmania major promastigotes and ass
115 could be enhanced in vitro and in vivo with L. amazonensis antigen-pulsed bone marrow-derived dendri