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1 L. mexicana Ag-stimulated lymph node cell culture from t
4 strong protection in recipient mice against L. mexicana infection, suggesting that attenuated L. don
5 ly acting and possessed good potency against L. mexicana (EC(50) = 120 nM), 30-fold selectivity for t
6 ma significantly enhances resistance against L. mexicana that is associated with a significant suppre
7 derivatives of 1 display IC50 values against L. mexicana GAPDH of 16 and 4 microM, respectively (3100
10 nlike mammalian aldolases, the T. brucei and L. mexicana aldolases contain nonameric N-terminal type
11 to use the X-ray structures of T. brucei and L. mexicana GAPDHs containing bound NAD+ to design adeno
12 nge 2-12 microM for T. brucei, T. cruzi, and L. mexicana GAPDH's, and these compounds did not inhibit
13 ngs of atypical cutaneous leishmaniasis, and L. mexicana from lesion aspirates from infected hamsters
15 peed videomicrographs showed that T. brucei, L. mexicana and a T. brucei RNAi morphology mutant have
18 ort a model in which ligation of FcgammaR by L. mexicana-bound immunoglobulin G promotes IL-10 produc
21 gh IL-10-deficient mice infected with either L. mexicana or L. amazonensis failed to control the lesi
22 y combining differential expression data for L. mexicana, L. major and Leptomonas seymouri, we have i
23 se-ubiquitin ligase (TKUL), is essential for L. mexicana to sustain macrophage infections and that TK
24 We have now solved the structure of ICP from L. mexicana by NMR and shown that it adopts a type of im
25 can provide protection against heterologous L. mexicana parasites by induction of a strong T cell re
26 copy cysteine protease B (CPB) gene array in L. mexicana is associated with decreased parasite virule
27 BTB 06237 also reduced parasite burdens in L. mexicana-infected J774 macrophages at low micromolar
28 cytokine production and profound defects in L. mexicana-specific Ig isotype class switching to IgG1
30 l, and genetic dissection of ARG function in L. mexicana promastigotes establishes: (i) that the enzy
31 by targeting single- and multi-copy genes in L. mexicana, L. major, L. donovani, and L. infantum, we
32 maRIII in suppressing protective immunity in L. mexicana infection, likely through macrophage IL-10 p
34 t a significant amount of the Ca2+ stored in L. mexicana amazonensis promastigotes and amastigotes is
36 ransfected with a cosmid expressing multiple L. mexicana CPB genes, this parasite induced a significa
37 c. inoculation with 5 x 10(6) amastigotes of L. mexicana into the shaven rump, STAT6+/+ mice develope
41 oach featuring arginase-deficient mutants of L. mexicana lacking both alleles of the gene encoding ar
42 L-4 signaling is critical for progression of L. mexicana infection in genetically susceptible mice an
43 ese data indicate that cysteine proteases of L. mexicana are critical in suppressing protective immun
44 of IL-10 or FcgammaR leads to resolution of L. mexicana disease and support a model in which ligatio
45 y reported orthorhombic crystal structure of L. mexicana GAPDH (LmGAPDH): the unusual conformation of
48 dependent upon IL-12p40, since treatment of L. mexicana-infected IL-10-/- mice with anti-IL-12p40 mo
50 n the case of L. mexicana complex parasites (L. mexicana, L. pifanoi, and L. amazonensis), a critical
51 espite ample levels of T cell proliferation, L. mexicana fails to induce substantial lymph node expan
53 Furthermore, B6 FcRgamma-/- mice resolve L. mexicana lesions, and lymph node cells from these mic
55 ishmaniasis caused by the New World species, L. mexicana and L. amazonensis, we analyzed their course
64 We now show that C3H mice infected with the L. mexicana deletion mutant (Deltacpb) initially develop
67 ow that FcgammaRIII KO mice are resistant to L. mexicana infection, resolving lesions in association
68 hermore, we found that T cells responding to L. mexicana infection were less able to differentiate in
71 stronger IgG2a/c, IgG3, and IgM responses to L. mexicana infection and yet are more resistant to the
72 y not be the mechanism for susceptibility to L. mexicana infection, unlike for L. major infection.
73 n was secreted/released by these transfected L. mexicana parasites and that it possessed functional c
77 results also provide an explanation for why L. mexicana CPA/CPB-deficient mutants transform to amast
79 her the nonhealing phenotype associated with L. mexicana was due to a failure of L. mexicana to activ
80 tion progressed, STAT6+/+ mice infected with L. mexicana displayed significantly higher titers of Lei
81 ceptibility of BALB/c mice to infection with L. mexicana and L. amazonensis, does play a significant