ライフサイエンスコーパス: LRP-1

1 LRP-1 also functions as a catabolic receptor for fibrone

2 LRP-1 also is implicated in integrin maturation.

3 LRP-1 expression on peripheral blood DCs was quantified

4 LRP-1 is not known to modulate the pathogenesis of aller

5 LRP-1, although abundant in brain microvessels in young

6 LRP-1-deficient macrophages, isolated from mice, demonst

7 LRP-1-deficient MEFs demonstrated increased Rac1 activat

8 LRP-1-deficient mouse embryonic fibroblasts lack a growt

9 sity lipoprotein receptor-related protein 1 (LRP-1) completely nullified the ability of tumour cells

10 sity lipoprotein receptor-related protein 1 (LRP-1) is a scavenger receptor that regulates adaptive i

11 ved that the LDL receptor-related protein 1 (LRP-1) is tyrosine phosphorylated in v-Src-transformed c

12 sity lipoprotein receptor-related protein 1 (LRP-1) receptor with receptor-associated protein (RAP) o

13 sity Lipoprotein Receptor-Related Protein 1 (LRP-1) receptor.

14 sity lipoprotein receptor-related protein 1 (LRP-1) was present during infection.

15 eceptor, the LDL receptor-related protein 1 (LRP-1).

16 eceptor, the LDL receptor-related protein 1 (LRP-1).

17 dies against LDL receptor-related protein-1 (LRP-1) and alpha(2)-macroglobulin (alpha(2)M).

18 r low-density lipoprotein-related protein-1 (LRP-1) from two smaller overlapping BACs ("BAC marriage"

19 sity lipoprotein receptor-related protein-1 (LRP-1) in NRK-49F cells, and this binding was competitiv

20 sity lipoprotein receptor-related protein-1 (LRP-1) is a catabolic receptor for extracellular matrix

21 sity lipoprotein receptor-related protein-1 (LRP-1) is a multifunctional receptor involved in recepto

22 sity lipoprotein receptor-related protein-1 (LRP-1) is expressed in adult sciatic nerve.

23 sity lipoprotein receptor-related protein-1 (LRP-1) mediates the endocytosis of multiple plasma membr

24 sity lipoprotein receptor-related protein-1 (LRP-1) plays a major role in TIMP-3 internalization.

25 ipoprotein (LDL) receptor-related protein-1 (LRP-1) to become enzymatically active.

26 sity Lipoprotein Receptor-Related Protein-1 (LRP-1) transcytosis pathway, amyloid beta (Abeta) peptid

27 LDL receptor (LDLR), LDLR-related protein-1 (LRP-1), and heparan sulfate proteoglycans (HSPGs), mostl

28 kinase AXL, LDL receptor-related protein-1 (LRP-1), and RAN-binding protein 9 (RANBP9)--that mediate

29 e in lipoprotein receptor related protein-1 (LRP-1)-targeted functionalized polymersomes in experimen

30 sity lipoprotein receptor-related protein-1 (LRP-1).

31 ts receptor, LDL receptor-related protein-1 (LRP-1).

32 sity lipoprotein receptor-related protein-1 (LRP-1).

33 Although LRP-1 functions as an endocytic receptor for C1r and C1s

34 Although LRP-1 gene silencing did not inhibit CL16 cell dissemina

35 g Schwann cell signaling and migration by an LRP-1-dependent pathway.

36 1 phosphorylation in vivo, we constructed an LRP-1 minireceptor composed of the beta chain linked at

37 cell survival, whereas re-introduction of an LRP-1 minigene in a mouse LRP-1-deficient fibroblast cel

38 RAP, an LRP-1 antagonist, inhibits binding of 125I-TGF-beta1 and

39 al fibroblasts and myofibroblasts through an LRP-1-, Erk1/2-, p90RSK-, and Bad-dependent mechanism.

40 AXL and LRP-1 did not interact directly, but relied on RANBP9, w

41 t relied on RANBP9, which bound both AXL and LRP-1, to form the complex.

42 P-1-negative mouse embryonic fibroblasts and LRP-1-deficient smooth muscle cells.

43 cs indicate that Glut4, the Tf receptor, and LRP-1 are differentially processed both within the cell

44 eceptor, a member of the same gene family as LRP-1, with overlapping ligand-binding specificity.

45 nce antagonists of receptor function such as LRP-1 and LRP-2 antibodies and the 39-kDa receptor-assoc

46 lture model of antigen presentation, the AXL/LRP-1/RANBP9 complex was used by DCs to cross-present AC

47 nt of beta(1) integrin was unchanged because LRP-1-deficient cells retained increased amounts of beta

48 n resonance to evaluate the affinity between LRP-1 and a number of MMPs, ADAMs, ADAMTSs, TIMPs and me

49 peptides shows that the interaction between LRP-1 and Shc is direct.

50 production of active heparanase by blocking LRP-1-mediated uptake of pro-heparanase and thereby decr

51 This response was blocked by LRP-1 gene silencing or by co-incubation with the LRP-1

52 the subsequent endocytosis of the complex by LRP-1 or LRP-2.

53 with receptor-associated protein (RAP) or by LRP-1 gene silencing.

54 volved in ECM modeling that are regulated by LRP-1.

55 olved in inflammation, that are regulated by LRP-1.

56 The cell surface receptor CD91/LRP-1 binds to immunogenic heat shock proteins (HSP) and

57 Ce-LRP-1 and Ce-LRP-2 possess a conserved intraluminal doma

58 the lipoprotein receptor-related proteins Ce-LRP-1 and Ce-LRP-2 and a cytoplasmic adaptor protein, Ce

59 Silencing of Schwann cell LRP-1 with siRNA decreased phosphorylated Akt and increa

60 embryonic fibroblasts (MEFs) and L929 cells, LRP-1 functions as a major regulator of Rac1 activation,

61 In uPAR-negative cells, LRP-1 neutralization does not affect Rac1 activation, an

62 ss from the brain largely via age-dependent, LRP-1-mediated transport that is influenced by alpha(2)M

63 rference screen using Caenorhabditis elegans LRP-1/megalin as a model for LDLR transport.

64 AP-D3 interfere with transport of endogenous LRP-1 to the cell surface in a dominant negative fashion

65 These results establish LRP-1 as a cell-signaling receptor for MMP-9, which may

66 h the observation that Schwann cells express LRP-1 at significant levels only after nerve injury.

67 First, LRP-1 stabilizes activated epidermal growth factor recep

68 In contrast, the k(ex) for LRP-1 was five times faster than Glut4 in basal cells, a

69 he affinity of target metalloproteinases for LRP-1, albeit to a lesser extent.

70 MP-1 was found to have a K(D) of 34.6 nM for LRP-1, while the MMP-1/TIMP-3 complex had a sevenfold hi

71 This identifies a novel role for LRP-1 as a negative regulator of DC-mediated adaptive im

72 factors identified here suggests a role for LRP-1 in determining blood vessel structure and in angio

73 nophilic asthma suggests a possible role for LRP-1 in modulating type 2-high asthma.

74 Furthermore, LRP-1-silenced cells expressed decreased levels of vascu

75 The megalin homologue LRP-1 is essential for growth and development in Caenorh

76 Human LRP-1 reversed the changes in protein expression associa

77 Expression of human LRP-1 in LRP-1-deficient MEFs reversed the shift in subc

78 Expression of human LRP-1 in LRP-1-deficient MEFs reversed the shift in subcellular b

79 JNK) blocked type III collagen expression in LRP-1-deficient MEFs, suggesting regulation of JNK activ

80 A substantial increase in LRP-1 expression was observed.

81 ) was mostly responsible for the increase in LRP-1 expression; this activity was reproduced by direct

82 nt protease mRNA expression was increased in LRP-1-deficient cells, as was expression of inducible ni

83 ptor, uPAR-AP/Endo-180, is also increased in LRP-1-deficient MEFs.

84 levels of cell surface beta(1) integrin, in LRP-1-deficient MEFs, were associated with decreased adh

85 t changes in cell signaling were observed in LRP-1-deficient murine embryonic fibroblasts.

86 inhibited NF-kappaB activity selectively in LRP-1-deficient cells.

87 ed into mature beta(1) (p125) more slowly in LRP-1-deficient cells.

88 Increased LRP-1 mRNA expression was observed locally at the injury

89 TNF-alpha also increased LRP-1 mRNA in Schwann cells in primary culture.

90 These findings show that tPA induces LRP-1 tyrosine phosphorylation, which in turn facilitate

91 After crush injury, LRP-1 is lost from the axoplasm and substantially upregu

92 ceptor responsible for ADAMTS-5 clearance is LRP-1.

93 mouse embryonic fibroblasts (PEA-13) lacking LRP-1, tPA failed to induce MMP-9 expression.

94 nervous system, did not bind to full-length LRP-1.

95 patients with eosinophilic asthma have lower LRP-1 expression than cells from healthy nonasthmatic su

96 Insulin increased k(en) for alpha-2-M/LRP-1 by 30%.

97 In basal cells, the k(en) for alpha-2-M/LRP-1 was similar to Glut4 but 5-fold slower than Tf.

98 (Tf) and alpha(2)-macroglobulin (alpha-2-M; LRP-1) were compared using quantitative flow cytometric

99 herefore, we hypothesized that DENV modifies LRP-1 protein expression to maintain host-derived intrac

100 introduction of an LRP-1 minigene in a mouse LRP-1-deficient fibroblast cell line (PEA-13) restored t

101 This identified MMP-1 as a new LRP-1 ligand.

102 Notably, LRP-1-mediated endocytosis of ADAMTS-5 is impaired in ch

103 The ability of LRP-1 to regulate expression of the factors identified h

104 ry mediators was explained by the ability of LRP-1 to suppress basal cell signaling through the I kap

105 To test the activity of LRP-1 in cancer progression in vivo, LRP-1 expression wa

106 ficiency; however, the endocytic activity of LRP-1 was not involved.

107 Neutralizing the activity of LRP-1 with receptor-associated protein (RAP) increased R

108 isease was assessed in mice with deletion of LRP-1 in CD11c(+) cells (Lrp1(fl/fl); CD11c-Cre) and by

109 esidues present in the cytoplasmic domain of LRP-1, only Tyr 63 is phosphorylated by v-Src in vivo or

110 protein containing the cytoplasmic domain of LRP-1, we show that LRP-1 is a direct substrate for v-Sr

111 ERK) that was responsible for the effects of LRP-1 on MEF migration.

112 1Lu cells selected for reduced expression of LRP-1 have an attenuated growth inhibitory response to T

113 Deletion or knockdown of LRP-1 abolished tPA-mediated cell survival, whereas re-i

114 We also found that dsRNA knockdown of LRP-1 increased intracellular cholesterol and DENV viral

115 t express very low or undetectable levels of LRP-1 when cultured in 21% O2 in vitro (standard cell cu

116 oximately 90% reduction in protein levels of LRP-1 without changes in its mRNA levels.

117 Absent or low levels of LRP-1, as with TbetaR-V, have been linked to the maligna

118 increase cAMP levels or inhibit migration of LRP-1-negative mouse embryonic fibroblasts and LRP-1-def

119 The role of LRP-1 in modulating HDM-induced airways disease was asse

120 ly slowed down after 10 h due to shedding of LRP-1 from the cell surface and formation of soluble LRP

121 osine phosphorylation on the beta subunit of LRP-1, which was followed by the activation of Mek1 and

122 1) integrin was most profoundly dependent on LRP-1 only after the cell cultures became confluent.

123 re expression of normal RAP has no effect on LRP-1 transport.

124 or with receptor-associated protein (RAP) or LRP-1 siRNA abolished the ApoE effects.

125 s present in the cell that can phosphorylate LRP-1.

126 Tyrosine-phosphorylated LRP-1 associates with Shc, a PTB and SH2 domain containi

127 In the peripheral nervous system (PNS), LRP-1 is robustly expressed by Schwann cells only after

128 unlike non-targeted carnosine polymersomes, LRP-1-targeted carriers accumulated in brain in a time d

129 ing Lys(1370) and Lys(1374), which precludes LRP-1 binding.

130 38B treatment, the receptor guidance protein LRP-1, which is required for endosomal recycling of the

131 aling pathway: LDL receptor-related protein (LRP-1) and decorin.

132 ensity lipoprotein receptor-related protein (LRP-1) and/or other cell surface receptors.

133 ensity lipoprotein receptor-related protein (LRP-1) binds and mediates the endocytosis of multiple li

134 ensity lipoprotein receptor-related protein (LRP-1) functions in endocytosis and in cell signaling di

135 ensity lipoprotein receptor-related protein (LRP-1) is an endocytic receptor for diverse proteins, in

136 ensity lipoprotein receptor-related protein (LRP-1), an endocytic receptor with cell signaling activi

137 analysis of a gp330/megalin-related protein, LRP-1, has been undertaken in Caenorhabditis elegans.

138 The adoptive transfer of HDM-pulsed LRP-1-deficient CD11b(+) DCs into WT mice generated a si

139 In addition to Rac1, LRP-1 suppressed activation of extracellular signal-regu

140 In rat kidney fibroblasts, tPA induced rapid LRP-1 tyrosine phosphorylation and enhanced beta1 integr

141 ion of the Abeta vascular clearance receptor LRP-1, and protection from the deleterious effects of Ab

142 M1 macrophage survival through its receptor LRP-1-mediated novel signaling cascade involving Erk1/2,

143 ine that binds to the cell membrane receptor LRP-1, induces its tyrosine phosphorylation, and trigger

144 cell migration through the surface receptor LRP-1 (LDL receptor-related protein 1)/CD91.

145 lized with LRP-1, and tPA deficiency reduced LRP-1/beta1 integrin interaction and myofibroblast activ

146 present during flavivirus infection reduced LRP-1 protein expression.

147 Furthermore, the reduced LRP-1 expression by circulating myeloid DCs in patients

148 gether, these data suggest that DENV reduces LRP-1 protein expression, possibly through regulated int

149 These activities require LRP-1.

150 We found that AXL bound ACs, but required LRP-1 to trigger internalization, in murine CD8alpha+ DC

151 Second, LRP-1 mediates secreted Hsp90alpha autocrine signalling

152 Silencing LRP-1 in PKM2-deficient macrophages restored inflammator

153 Similarly, LRP-1 expression by CD11b(+) lung DCs was significantly

154 om the cell surface and formation of soluble LRP-1 (sLRP-1)-TIMP-3 complexes.

155 virus-1 infection, mice lacking DC-specific LRP-1, AXL, or RANBP9 had increased AC accumulation, def

156 To study LRP-1 phosphorylation in vivo, we constructed an LRP-1 m

157 alpha action, or removal of the cell surface LRP-1 receptor for secreted Hsp90alpha reduces the tumor

158 ehind the important function by cell surface LRP-1 was not fully understood.

159 short gluten peptide that leverages the TG2/LRP-1 pathway.

160 We hypothesized that LRP-1 may down-regulate cell surface beta(1) by promotin

161 These observations indicate that LRP-1 is related to megalin not only structurally but al

162 We propose that LRP-1 suppresses expression of inflammatory mediators in

163 We show that LRP-1 deficiency in murine embryonic fibroblasts (MEFs)

164 he cytoplasmic domain of LRP-1, we show that LRP-1 is a direct substrate for v-Src in vitro.

165 In this study, we show that LRP-1 is abundantly expressed in severe combined immunod

166 We show that LRP-1 is associated with endothelial transcytosis that d

167 Herein we show that LRP-1 orchestrates two parallel cell surface signalling

168 cause recent studies in rodents suggest that LRP-1 inhibits inflammation, we conducted activity-based

169 Our observations suggest that LRP-1 may be involved in normal and malignant signal tra

170 enocopy the lrp-1 mutations, suggesting that LRP-1 is a receptor for sterols that must be endocytosed

171 Previous literature suggests that LRP-1 regulates cholesterol homeostasis.

172 This suggests that LRP-1 scavenging of TIMP/metalloproteinase complexes may

173 The LRP-1-targeted functionalization was essential for deliv

174 essed the growth factor carrier site and the LRP-1 recognition domain (RBD) as separate GST fusion pr

175 blockade of the tumour cell migration as the LRP-1 depletion did.

176 Both apoE and pro-heparanase bind the LRP-1.

177 s binding was competitively abrogated by the LRP-1 antagonist, the receptor-associated protein.

178 osphorylation, which in turn facilitates the LRP-1-mediated recruitment of beta1 integrin and downstr

179 ta1 integrin recruitment by facilitating the LRP-1/beta1 integrin complex formation.

180 l death was induced in vivo by injecting the LRP-1 antagonist, receptor-associated protein, into axot

181 These results suggest that the LRP-1/TbetaR-V/IGFBP-3 receptor is required for the grow

182 gene silencing or by co-incubation with the LRP-1 antagonist, receptor-associated protein.

183 Thus, LRP-1 dictates physiological and pathological catabolism

184 Thus, LRP-1 regulates two signaling proteins in the same cell

185 arly bridged binding of MMP-13 and MMP-14 to LRP-1.

186 cells is immunoprecipitated by antibodies to LRP-1 and TbetaR-V.

187 acids, is responsible for decorin binding to LRP-1 and subsequent TGF-beta-dependent signaling.

188 tracellular pathway by which apoE binding to LRP-1 results in inhibition of smooth muscle cell migrat

189 were blocked by inhibiting MMP-9-binding to LRP-1 with receptor-associated protein (RAP) or by LRP-1

190 teases, the thiol ester bonds, or binding to LRP-1.

191 ctivation as a consequence of its binding to LRP-1.

192 alloproteinases by bridging their binding to LRP-1.

193 Among the proteins analyzed, TIMP-3 bound to LRP-1 with highest affinity (K(D) = 1.68 nM).

194 etaR-V was recently found to be identical to LRP-1.

195 we demonstrate that TbetaR-V is identical to LRP-1/alpha2M receptor as shown by MALDI-TOF analysis of

196 In mouse obstructed kidney, tPA, LRP-1, and MMP-9 were concomitantly induced in the renal

197 The ability of FP6 to trigger LRP-1-dependent cell signaling in PC12 cells was reprodu

198 its internalization; however, unexpectedly, LRP-1 expression was associated with a substantial incre

199 ism by which certain breast cancer cells use LRP-1 to engage parallel signalling pathways to move whe

200 ith insulin, (Q3A4Y15L16) IGF-I, or TbetaR-V/LRP-1 antagonists.

201 and IRS-2 are key molecules for the TbetaR-V/LRP-1-mediated growth inhibitory signaling cascade.

202 vity of LRP-1 in cancer progression in vivo, LRP-1 expression was silenced in CL16 cells with short h

203 rawal or TNF-alpha, to a greater extent when LRP-1 was silenced.

204 roteases accumulate at increased levels when LRP-1 is absent.

205 antly increased cell death was observed when LRP-1-silenced CL16 cells were exposed to CoCl2, which m

206 We sought to assess whether LRP-1 expression by dendritic cells (DCs) modulates adap

207 f the present study was to determine whether LRP-1 regulates cell surface levels of the beta(1) integ

208 To test whether LRP-1 expression in vivo may be explained by hypoxia in

209 tion of JNK activity as a mechanism by which LRP-1 controls mRNA expression.

210 The mechanism by which LRP-1 inhibits NF-kappaB activity involves down-regulati

211 These results suggest mechanisms by which LRP-1 may facilitate the development and growth of cance

212 c1 activation, and other mechanisms by which LRP-1 may regulate cell migration are not unmasked.

213 These studies support a model in which LRP-1 either directly or indirectly promotes maturation

214 e cells formed tumors in SCID mice, in which LRP-1 expression remained silenced.

215 These results support a model in which LRP-1 functions as a pro-survival receptor in Schwann ce

216 A mutagen-treated CHO cell line, in which LRP-1 is expressed but retained in the secretory pathway

217 hanges in protein expression associated with LRP-1 deficiency; however, the endocytic activity of LRP

218 ion of H1299 human lung carcinoma cells with LRP-1 cDNA restores the growth inhibitory response.

219 e injury, tPA and alpha-SMA colocalized with LRP-1, and tPA deficiency reduced LRP-1/beta1 integrin i

220 ated increased Rac1 activation compared with LRP-1-expressing MEFs, and this property was reversed by

221 in that are responsible for interaction with LRP-1 and are involved in TGF-beta-dependent binding and

222 and LRR5 participate in the interaction with LRP-1 and TGF-beta as well as in its dependent signaling

223 f MMP-9, which mediates the interaction with LRP-1, blocked MMP-9-induced cell signaling and migratio