ライフサイエンスコーパス: LTC4

1 oinflammatory lipid mediator leukotriene C4 (LTC4).

2 or the glutathione conjugate leukotriene C4 (LTC4).

3 he biosynthesis of cysteinyl leukotriene C4 (LTC4).

4 e the proinflammatory signal leukotriene C4 (LTC4).

5 jugation of LTA4 with glutathione to produce LTC4.

6 which binds glutathione conjugates including LTC4.

7 letely the augmented secretion of eosinophil LTC4.

8 ecific tissue to respond to LT, specifically LTC4.

9 Photoaffinity labeling of HL-60 cells with LTC4-125I-ASA identified GAPDH as the predominant cytopl

10 5I]4-azidosalicylic acid (PNBG-[125I]ASA) or LTC4-[125I]4-azidosalicylic acid (LTC4-[125I]ASA) was 38

11 LTC4-[125I]ASA binding to the NAD+ site was confirmed by

12 ase digestion of purified GAPDH labeled with LTC4-[125I]ASA or PNBG-[125I]ASA, with both labels local

13 5I]ASA) or LTC4-[125I]4-azidosalicylic acid (LTC4-[125I]ASA) was 38 kDa.

14 inyl leukotrienes (cys-LTs), leukotriene C4 (LTC4), a conjugation product of glutathione and eicosate

15 riene C4 (LTC4) and require STIM1 downstream LTC4 action.

16 apt1 demonstrated higher uptake of 10 nM [3H]LTC4 and 50 microM [3H]DNP-SG, and higher efflux of [3H]

17 The oatp1-stimulated LTC4 and DNP-SG uptake was independent of the Na+ gradie

18 C2s were increased after challenge with both LTC4 and IL-33.

19 an macrophages with LTC4S inhibitors blocked LTC4 and increased resolvins and lipoxins.

20 Both LTC4 and LTD4 enhanced endothelial adhesiveness for PMN,

21 or the type 1 cys-LT receptor (CysLT1R), and LTC4 and LTD4 have similar lesser potency for CysLT2R, w

22 In contrast, LTC4 and LTD4 induced concentration-dependent contractio

23 ry artery ring segments were unresponsive to LTC4 and LTD4.

24 epicardial coronary arteries in response to LTC4 and LTD4.

25 sting that the MRP/GS-X pump transports both LTC4 and the GS-Pt complex.

26 However, the source of LTC4 and the signaling mechanisms of STIM1 in the activa

27 The permeability response to exogenous LTC4 and to endogenous CysLTs evoked by passive cutaneou

28 FMLP) induces the release of leukotriene C4 (LTC4) and histamine in human basophils.

29 Leukotriene C4 (LTC4) and its extracellular metabolites, LTD4 and LTE4,

30 ein.The peptidoleukotrienes, leukotriene C4 (LTC4) and leukotriene D4 (LTD4) also provoked PMN-endoth

31 the contractile responses of leukotriene C4 (LTC4) and leukotriene D4 (LTD4) and their binding activi

32 nnels are gated by cytosolic leukotriene C4 (LTC4) and require STIM1 downstream LTC4 action.

33 athione S-conjugates such as leukotriene C4 (LTC4) and S-dinitrophenyl glutathione (DNP-SG) form a no

34 macrophages produced leukotriene B4 (LTB4), LTC4, and 5-hydoxyeicosatetraenoic acid (5-HETE) during

35 M, for daunorubicin, etoposide, vincristine, LTC4, and ATP, respectively.

36 m mobilization) of this receptor by LTD4 and LTC4, and competition for radiolabelled LTD4 binding to

37 DNP-SG, LTC4, and E(2)17betaG are transported at moderate affini

38 Alternaria alternata Thus, CysLT1R promotes LTC4- and Alternaria-induced ILC2 activation and lung in

39 In leukotriene-competent cells, LTC4 augmented phagocytosis to the greatest extent, foll

40 ed NBD2, the inhibitory effect of ATP on the LTC4 binding is significantly decreased.

41 ion autoradiography identified specific [3H]-LTC4 binding sites to smooth muscle cell and to regions

42 Specific [3H]-LTC4 but not LTD4 binding to atherosclerotic coronary ar

43 In this article, we demonstrate that LTC4, but not LTD4 or LTE4, activates mouse platelets ex

44 ore the resulting products thromboxane A2 or LTC4 can activate their cognate receptors.

45 4+ T cells augmented eosinophil secretion of LTC4 caused by FMLP plus cytochalasin.

46 rovocation in patients with asthma increases LTC4 concentrations in bronchoalveolar lavage fluid, whi

47 LTC4-converting activity has a tissue distribution diffe

48 LTC4/CysLT2R signaling required an autocrine ADP-mediate

49 ein expression and higher levels of LTB4 and LTC4/D4/E4 emerged in children with obstructive sleep ap

50 nd LT2-R, and for concentrations of LTB4 and LTC4/D4/E4.

51 Leukotriene-C4 synthase (LTC4S) generates LTC4 from arachidonic acid metabolism.

52 hVPLA2 also augmented the release of AA and LTC4 from eosinophils activated with formyl-Met-Leu-Phe

53 dhesion up-regulated stimulated secretion of LTC4 from eosinophils.

54 the roles of P-gp and MRP in the release of LTC4 from normal murine mast cells (MC-9).

55 to elicit the significant release of AA and LTC4 from unstimulated eosinophils, which depended on it

56 aused augmented secretion of leukotriene C4 (LTC4) from eosinophils.

57 PD98059 also inhibited LTC4 generation (IC50 = approximately 2 microM), but not

58 kinase/ERK kinase) and ERKs while inhibiting LTC4 generation as well as histamine release.

59 selectively regulate the pathway leading to LTC4 generation by phosphorylating cPLA2, but not histam

60 RK1/2) specifically regulate the pathway for LTC4 generation, but not for histamine release and inter

61 203347 did not alter A23187-induced PGE2 or LTC4 generation, respectively, indicating that these age

62 enges with the parent CysLT, leukotriene C4 (LTC4), given in combination with low-dose IL-33 to naive

63 ion to GGT at least one other enzyme cleaves LTC4 in mice.

64 levels, we measured IL-33-induced histamine/LTC4 in vitro, CD63 translocation ex vivo, and responsiv

65 vestigated the metabolism of leukotriene C4 (LTC4) in gamma-glutamyl transpeptidase (GGT)-deficient m

66 f ATP-dependent transport of leukotriene C4 (LTC4) in plasma membrane vesicles.

67 ophils secrete histamine and leukotriene C4 (LTC4) in response to various stimuli, such as Ag and the

68 LTC4 induced surface expression of CD62P by wild-type mo

69 ed guinea pig trachea, but do not antagonize LTC4-induced contractions, which are putatively mediated

70 Deletion of Cysltr1 blunted LTC4-induced ILC2 expansion and eosinophilia but did not

71 LTC4 is a proinflammatory factor that acts on plasma mem

72 ere, we show that upon thrombin stimulation, LTC4 is produced through the sequential activities of ph

73 These data suggest that LTC4 is secreted by normal mast cells by a probenecid-se

74 LTC4 is the parent molecule of the cysteinyl leukotriene

75 The cysteinyl leukotrienes-leukotriene C4(LTC4), leukotriene D4(LTD4) and leukotriene E4(LTE4)-are

76 The restorative and pharmacologic effects of LTC4, LTB4, and 5-HETE may provide a basis for their exo

77 einyl leukotrienes (cysLTs), leukotriene C4 (LTC4), LTD4, and LTE4 are proinflammatory lipid mediator

78 Leukotrienes (LTA4, LTB4, LTC4, LTD4, and LTE4) are inflammatory lipid mediators.

79 comprising the cysteinyl leukotrienes (LTs; LTC4, LTD4, and LTE4), only LTE4 is stable and abundant

80 Co-application of LTB4 with LTA4, LTC4, LTD4, or LTE4 suppressed LTB4-induced activation.

81 Nascent LTC4 may activate platelets at a synapse with granulocyt

82 ng to the synthesis of leukotrienes LTB4 and LTC4, mediators of inflammation and pain, were found in

83 nt vascular leak to intradermal injection of LTC4 or LTD4 and an augmented response to LTE4 as compar

84 4-mediated vascular permeability, but not to LTC4 or LTD4, revealing a preference of GPR99 for LTE4 e

85 LTC4 , PGE2 , and 11-dehydro-TXB2 did not differ between

86 LTC4 potentiated airway inflammation in a platelet- and

87 Also, inhibition of LTC4 production caused by adherence could not be overcom

88 ntrast, neither treatment altered stimulated LTC4 production.

89 nd degranulation while negatively regulating LTC4 production.

90 echanisms of STIM1 in the activation of this LTC4-regulated Ca(2+) (LRC) channel are unknown.

91 d messenger that activated store-independent LTC4-regulated Ca(2+) (LRC) channels encoded by Orai1/Or

92 to FMLP, PD98059 inhibited anti-IgE-mediated LTC4 release (IC50, approximately 2 microM), with only a

93 as kinetically similar to both histamine and LTC4 release and decreased toward resting levels by 30 m

94 898 (IC(50) = 900 nM) in a human whole blood LTC4 release assay.

95 se A2 (cPLA2), and arachidonic acid (AA) and LTC4 release followed phosphorylation of ERKs.

96 lls, suggesting that P-gp is not involved in LTC4 release from MC-9 cells.

97 enge in OVA-sensitized mice induced LTB4 and LTC4 release into the airspace, widespread mucus occlusi

98 mediated responses, including degranulation, LTC4 release, and IL-13 production in BMMCs through the

99 RK-2, which are required for leukotriene C4 (LTC4) release, and production of LTC4 was increased 3- t

100 Elevated oesophageal expression of LTC4 S mRNA is found in a subgroup of EoE patients, conc

101 LTC4 S mRNA levels were elevated in proximal (2.6-fold,

102 LTC4 S mRNA levels were quantified in whole blood sample

103 with TSLP and IL-23 mRNA levels, oesophageal LTC4 S mRNA may facilitate diagnosis of an EoE subpopula

104 Importantly, increased LTC4 S mRNA transcripts identified a subpopulation of Eo

105 LTC4 S mRNA transcripts in whole blood and urinary excre

106 ther mRNA levels of leukotriene C4 synthase (LTC4 S), a key regulator of leukotriene production, coul

107 In addition to LTC4, S-decyl-GSH is also cleaved.

108 LTC4-S enzyme activity in the microsomal fraction of hep

109 reaction (RT-PCR) showed that LPS increased LTC4-S expression in hepatocytes by a factor of 3 (n = 3

110 LPS enhances LTC4-S expression in hepatocytes.

111 LTC4-S mRNA was detected in whole liver, hepatocytes, an

112 es, 5-lipoxygenase (5-LO) and LTC4 synthase (LTC4-S), in liver cells has never been demonstrated.

113 however, when corrected for cell viability, LTC4 secretion decreased from 1,429 +/- 327 pg/10(6) cel

114 At 24 h, stimulated LTC4 secretion from eosinophils was unchanged; however,

115 a concentration-dependent manner, inhibited LTC4 secretion from MC-9 cells without inhibiting its sy

116 CsA and CsA-1 had no effect on LTC4 secretion from MC-9 cells, suggesting that P-gp is

117 version of leukotriene A4 to leukotriene C4 (LTC4), suggesting a role for the activity of LTC4 syntha

118 synthesis enzymes, 5-lipoxygenase (5-LO) and LTC4 synthase (LTC4-S), in liver cells has never been de

119 the terminal enzyme for cysLT biosynthesis, LTC4 synthase (LTC4S).

120 Adherence increased, rather than decreased, LTC4 synthase activity.

121 e- and dose-dependent increase in stimulated LTC4 synthase activity.

122 The intron/exon junctions of LTC4 synthase align identically with those of FLAP; howe

123 m a P1 library now reveals that the gene for LTC4 synthase contains five exons (ranging from 71 to 25

124 Previous cloning of the cDNA for human LTC4 synthase demonstrated significant homology of its a

125 We conclude that LTC4 synthase expression is increased at a transcription

126 hose of FLAP; however, the small size of the LTC4 synthase gene contrasts with the > 31-kilobase pair

127 Confirmation of the LTC4 synthase gene size to ensure that no deletions had

128 ogression was partially reproduced in global LTC4 synthase KO or mice transplanted with LTA4 hydrolas

129 eta resulted in a time-dependent increase in LTC4 synthase mRNA at 6 h, which persisted through 48 h.

130 nel of cytokines was surveyed for changes in LTC4 synthase mRNA in THP-1 cells.

131 th TGF-beta did not prolong the half-life of LTC4 synthase mRNA, as assessed by RNase protection assa

132 e observed stimulatory effect of TGF-beta on LTC4 synthase mRNA.

133 -reporter construct containing 1.2 kb of the LTC4 synthase promoter, TGF-beta treatment resulted in a

134 in an increase in immunoreactive protein for LTC4 synthase through 7 days.

135 Fluorescent in situ hybridization mapped LTC4 synthase to chromosomal location 5q35, which is in

136 lacking CysLT1R and the biosynthetic enzyme LTC4 synthase, implying a requirement for a cys-LT-media

137 nd subsequent activity of LTA4 hydrolase and LTC4 synthase, respectively.

138 LTC4), suggesting a role for the activity of LTC4 synthase.

139 Leukotriene C4 (LTC4) synthase (LTC4S), an integral membrane protein, ca

140 Leukotriene C4 (LTC4) synthase catalyzes the conjugation of LTA4 with re

141 e whether cytokines modulate leukotriene C4 (LTC4) synthase expression in mononuclear phagocytes.

142 Inhibition of LTC4 synthesis precedes the global cytotoxic effects of

143 eated with > 10(-)10 M FP, and inhibition of LTC4 synthesis was reversed by exogenous arachidonic aci

144 ar redistribution of 5-LO and an increase in LTC4 synthesis.

145 denced by its translocation to membranes and LTC4 synthesis.

146 nce also caused a time-dependent decrease in LTC4 synthesis: EOS adhered for 120 min produced 90% les

147 (cPLA2), and (2) blockade of leukotriene C4 (LTC4) synthesis in isolated human eosinophils in vitro.

148 idonic acid (AA) release and leukotriene C4 (LTC4) synthesis in isolated human peripheral blood eosin

149 s: EOS adhered for 120 min produced 90% less LTC4 than did cells adhered for 5 min.

150 crease in stimulated eosinophil secretion of LTC4 that is regulated by phospholipase A2 (PLA2).

151 Administrations of either LTC4 (the parent cysLT) or the selective CysLT2R agonist

152 l S-glutathione (DNP-SG) and leukotriene C4 (LTC4), the antimetabolite methotrexate, and the bile aci

153 d 20-carboxy-LTB4, the cysteinyl leukotriene LTC4, the 15-lipoxygenase product 15(S)-HETE, or the lip

154 kotriene A4 with reduced glutathione to form LTC4, the biosynthetic parent of the additional cysteiny

155 acid LTA4 with the tripeptide GSH to produce LTC4, the parent compound of the cysteinyl leukotrienes,

156 We recently found that LTC4, the parent cysLT, potently activates platelets in

157 conjugation of LTA4 with reduced GSH to form LTC4, the parent of the receptor active cysteinyl leukot

158 T) or the selective CysLT2R agonist N-methyl LTC4 to allergen sensitized wild-type mice markedly boos

159 ematopoietic TP receptors were essential for LTC4 to induce eosinophil recruitment.

160 ice and have found substantial conversion of LTC4 to leukotriene D4 by high performance liquid chroma

161 Intranasal administration of LTC4 to OVA-sensitized C57BL/6 mice markedly increased t

162 trienase (GGL) that converts leukotriene C4 (LTC4) to leukotriene D4 (LTD4).

163 ases the affinity for ATP, the ATP-dependent LTC4 transport activities are much higher than that of w

164 droxyl group at this position, exerts higher LTC4 transport activity than the wild-type MRP1, indicat

165 on the Kd value, the Km values measured from LTC4 transport by proteins with this mutation in NBD2 ar

166 ing/hydrolysis at NBD2; and 2) ATP-dependent LTC4 transport by the protein with a cysteine residue in

167 In contrast, the Km for ATP in support of LTC4 transport is mainly determined by ATP hydrolysis at

168 , but not cisplatin, inhibited ATP-dependent LTC4 transport, suggesting that the MRP/GS-X pump transp

169 most completely abolishing the ATP-dependent LTC4 transport.

170 inding or Km values for ATP in ATP-dependent LTC4 transport.

171 2 plays a dominant role in the ATP-dependent LTC4 transport.

172 tly affect the ATP-dependent leukotriene C4 (LTC4) transport.

173 did not affect ATP-dependent leukotriene C4 (LTC4) transport.

174 unctions of thromboxane A2 act downstream of LTC4/type 2 cysLT receptor signaling on platelets to mar

175 LTC4 upregulated the expressions of ICAM-1 and VCAM-1 in

176 ignificance, oatp1-mediated taurocholate and LTC4 uptake was cis-inhibited and trans-stimulated by GS

177 Recently, however, we showed that LTC4 was also a cytosolic second messenger that activate

178 id, systemic pressor response to intravenous LTC4 was also diminished in TG mice coincidentally with

179 LTC4 was fully active on PRP from mice lacking either Cy

180 otriene C4 (LTC4) release, and production of LTC4 was increased 3- to 5-fold, compared with cells tra

181 Culture supernatants were collected, and LTC4 was measured by ELISA assay.

182 implicated in the release of leukotriene C4 (LTC4), we examined the roles of P-gp and MRP in the rele

183 erted into the cysteinyl leukotriene (cysLT) LTC4, which is converted into LTD4 and finally to LTE4 a

184 The synergistic effect of LTC4 with IL-33 was completely dependent upon CysLT1R, b

185 Thus, CysLT2R on platelets recognizes LTC4 with unexpected selectivity.