ライフサイエンスコーパス: M. genitalium

1 levant clinical samples for the detection of M. genitalium.

2 and 7 of 10 (70%) participants positive for M. genitalium.

3 ational repair pathway plays a minor role in M. genitalium.

4 tly more common among subjects infected with M. genitalium.

5 Inflammation was highest among subjects with M. genitalium.

6 s conserved in other bacteria are missing in M. genitalium.

7 o model and assess endocervical infection by M. genitalium.

8 . vaginalis, and 47 (9.5%) were positive for M. genitalium.

9 es of four geographically diverse strains of M. genitalium.

10 ly conserved in other bacteria are absent in M. genitalium.

11 of MG_454 resists organic hydroperoxides in M. genitalium.

12 n-house DNA-based PCR assay for detection of M. genitalium.

13 ypes and the MG309 repeats for genotyping of M. genitalium.

14 ndently increased the odds of infection with M. genitalium.

15 peroxide when compared to wild-type virulent M. genitalium.

16 U were infected with both C. trachomatis and M. genitalium.

17 lest sequenced prokaryotic genome except for M. genitalium.

18 srupt the cytadherence-related gene mg218 of M. genitalium.

19 nvestigating the biology and pathogenesis of M. genitalium.

20 ith total RNA isolated from M. pneumoniae or M. genitalium.

21 to have been exposed to sexually transmitted M. genitalium.

22 ermine minimum inhibitory concentrations for M. genitalium.

23 and widely used to study the epidemiology of M. genitalium.

24 sure optimal selection of antimicrobials for M. genitalium.

25 limited availability of diagnostic tests for M. genitalium.

26 lification NAATs targeting unique regions of M. genitalium 16S or 23S rRNA.

27 mplification (TMA) NAAT for the detection of M. genitalium 16S rRNA.

28 We observed a high prevalence of M. genitalium (7.4%) among HIV-infected New Orleans wome

29 s a tool to analyze the function of genes in M. genitalium, a plasmid that replicates in Escherichia

30 terested in defining the mechanisms by which M. genitalium adheres to and colonizes host cell surface

31 with a 3.5-fold increase in odds of incident M. genitalium (adjusted odds ratio = 3.49, 95% confidenc

32 Mice infected with M. genitalium also developed specific antibodies to the

33 Screening and treatment of M. genitalium among HIV-infected individuals may be warr

34 tic (IVD) TMA test that targets 16 s rRNA of M. genitalium Analytical sensitivity, specificity, and s

35 determined using nine laboratory strains of M. genitalium and 56 nontarget bacteria, protozoa, and v

36 lable molecular methods for the diagnosis of M. genitalium and assays to predict the antibiotic susce

37 Prevalence of M. genitalium and associated genotypic markers of macrol

38 e recently developed assay can test for both M. genitalium and azithromycin resistance mutations at t

39 V)-infected women for an association between M. genitalium and cervicitis, a putative mechanism for e

40 cally diverse clinical sites were tested for M. genitalium and for Chlamydia trachomatis, Neisseria g

41 M. genitalium and H. influenzae belong to two ancient ba

42 growing evidence for an association between M. genitalium and HIV genital shedding and the high prev

43 he strong epidemiologic associations between M. genitalium and human immunodeficiency virus (HIV), pr

44 tions in the completely sequenced genomes of M. genitalium and its close relative M. pneumoniae were

45 that groups of functionally related genes in M. genitalium and M. pneumoniae are often preceded by pr

46 Cases were tested for M. genitalium and macrolide-resistance mutations (MRMs)

47 nes, many of which appear to be essential in M. genitalium and other bacteria.

48 Samples were tested for M. genitalium and other STI organisms (Chlamydia trachom

49 ging data demonstrate an association between M. genitalium and PID, and limited data suggest associat

50 leans women, we retrospectively screened for M. genitalium and quantitatively characterized several m

51 carriers are about twofold more prevalent in M. genitalium and Synechocystis.

52 The M. genitalium and T. vaginalis detection rates among 755

53 The associations between M. genitalium and the detection and quantity of genital

54 cells expressing specific human TLR, viable M. genitalium and the recombinant C-terminal portion of

55 d pathways involved in innate recognition of M. genitalium and the response to acute infection in the

56 ervical EC are immunologically responsive to M. genitalium and to purified rMG309c via highly express

57 xpansion of the STI analyte panel (including M. genitalium) and additional specimen source sampling w

58 anococcus jannaschii, Mycoplasma pneumoniae, M. genitalium, and Synechocystis PCC6803.) These repeats

59 redibly small-two to four times smaller than M. genitalium-and these tiny genomes have raised questio

60 relationships of the PDB domains that match M. genitalium are described in the structural classifica

61 Because B. burgdorferi and M. genitalium are distantly related eubacteria, we sugge

62 65 to 350 of the 480 protein-coding genes of M. genitalium are essential under laboratory growth cond

63 he epidemiology and clinical significance of M. genitalium as a human pathogen made possible by the a

64 discovery, microbiology, and recognition of M. genitalium as a pathogen, and then summarize the rece

65 These findings implicate M. genitalium as an etiologic agent of cervicitis in HIV

66 ug Administration-approved clinical test for M. genitalium available in the United States at this tim

67 ibited a range of antibody responsiveness to M. genitalium based upon ELISA and immunoblot assessment

68 upstream of these two genes was detected in M. genitalium but not M. pneumoniae.

69 he small genome organisms, H. influenzae and M. genitalium, but is totally lacking in H. pylori, Syne

70 ces cumulative PID incidence in women due to M. genitalium by 31.1% (95% range:13.0%-52.0%) over 20 y

71 hat MsrA affects the virulence properties of M. genitalium by modulating its interaction with host ce

72 from women every other month were tested for M. genitalium by nucleic acid amplification testing.

73 cts were also positive for M. fermentans and M. genitalium by PCR.

74 s to Statens Serum Institut for detection of M. genitalium by polymerase chain reaction between 1 Jan

75 generation of genetic diversity observed in M. genitalium by the mutual exchange of sequences makes

76 ed field, the positive predictive values for M. genitalium, C. trachomatis, N. gonorrhoeae, and T. va

77 Here, we report that M. genitalium can establish long-term infection of human

78 gs provide strong experimental evidence that M. genitalium can establish long-term infection of repro

79 The evidence that M. genitalium causes epididymo-orchitis, proctitis, and

80 into wild-type hemadsorption-positive (HA+) M. genitalium cells permitted the isolation of HA- (stra

81 Specimens were tested for M. genitalium, Chlamydia trachomatis, Neisseria gonorrho

82 Additionally, the isolation of single-colony M. genitalium clonal variants containing alternative mgp

83 i RNA from five M. pneumoniae clones and two M. genitalium clones indicated that transcription origin

84 hase inflammatory responses and suggest that M. genitalium colonization of reproductive tract tissues

85 M. genitalium colonizes host cells primarily through adh

86 (HIV) infection revealed 19 (18.6%) cases of M. genitalium, commonly (58%) in rectal samples.

87 An msrA disruption mutant of M. genitalium, constructed through homologous recombinat

88 ys (ELISAs) and immunoblot and PCR assays in M. genitalium culture-positive women over 1 to 3 years o

89 evidence for the first FDA-cleared NAAT for M. genitalium detection in the United States.

90 re was no evidence of an association between M. genitalium detection or quantity and either plasma HI

91 A 10.0% M. genitalium detection rate from other facilities excee

92 sing the search terms Mycoplasma genitalium, M. genitalium, diagnosis, and detection.

93 se as a comparator for the validation of new M. genitalium diagnostic tests.

94 Typing assigned M. genitalium DLSTs to 2 major clusters, broadly distrib

95 Libraries of M. pneumoniae and M. genitalium DNA constructed in pGFPUV2 and transformed

96 ative polymerase chain reaction specific for M. genitalium DNA on samples 14-100 days post-treatment.

97 ormat and performance indices of a number of M. genitalium DNA- and RNA-based amplification assays; m

98 ense and inflammation signature activated by M. genitalium during acute infection (48 hours after ino

99 ption-mediated amplification (TMA) tests for M. genitalium, each targeting unique rRNA sequences, for

100 tudy, we demonstrate that surface-associated M. genitalium EF-Tu (EF-Tu(Mg)), in spite of sharing 96%

101 e stress response regulator in the genome of M. genitalium, elevated expression of MG_454 due to phys

102 tions, understanding the mechanisms by which M. genitalium elicits mucosal inflammation is an essenti

103 arative genomics has revealed that MG_454 of M. genitalium encodes a protein with putative function a

104 of identified STI involved sole detection of M. genitalium Expansion of the STI analyte panel (includ

105 Here, we show that M. genitalium expresses N-terminally truncated RecA isof

106 to doxycycline for presumptive treatment of M. genitalium, followed by resistance-guided therapy, cu

107 Recovery of viable M. genitalium from lower genital tract specimens was imp

108 CVI-1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by a

109 Although the M. genitalium gene complement is indeed the smallest amo

110 We found that 240 M. genitalium genes have orthologs among the genes of H.

111 ted the set of orthologs with the respective M. genitalium genes.

112 f chaperone expression]) is predicted in the M. genitalium genome as well as three copies of its corr

113 Approximately 4% of the limited M. genitalium genome contains repeat sequences termed Mg

114 city of recombination genes conserved in the M. genitalium genome.

115 Several novel M. genitalium genotypes were identified, all of which we

116 Disruption of some genes accelerated M. genitalium growth.

117 omes of sequential antimicrobial therapy for M. genitalium guided by a macrolide-resistance assay.

118 ence of known cervical pathogens, women with M. genitalium had a 3.3-fold greater risk (95% confidenc

119 ke Escherichia coli, inactivation of recA in M. genitalium had a minimal effect on survival after exp

120 Testing for M. genitalium has become important for clinicians treati

121 ng available, and their use in screening for M. genitalium has been advocated, but M. genitalium's na

122 Detection of M. genitalium has relied almost entirely on PCR amplific

123 It appears plausible that M. genitalium has the ability to generate unlimited vari

124 Since M. genitalium has the capacity to invade eukaryotic cell

125 However, diagnostic assays for M. genitalium have been limited in the United States.

126 which provides insights into the dynamics of M. genitalium-host interactions.

127 of MG_454 to respond to oxidative stress in M. genitalium implies the absence of a known oxidative s

128 screening programs and targeted treatment of M. genitalium improve reproductive outcomes in women are

129 gpB sequences vary within a single strain of M. genitalium in a pattern consistent with recombination

130 comprehensive testing programs would detect M. genitalium in a significant proportion of females, pa

131 ata which directly implicate the presence of M. genitalium in disease pathogenesis.

132 g and the high prevalence and persistence of M. genitalium in this population suggest that further re

133 sensitive specimen type for the detection of M. genitalium in women.

134 evealed reinfection by a different strain of M. genitalium, indicating the absence of protective immu

135 a high azithromycin failure rate (39%) in an M. genitalium-infected cohort in association with high l

136 The mean age of the M. genitalium-infected females (24.7 years) was lower th

137 Consecutive eligible M. genitalium-infected men and women attending the Melbo

138 Analysis of 31 M. genitalium-infected patient specimens and 7 American

139 e strain and in sequential specimens from an M. genitalium-infected patient.

140 4.3]), C. trachomatis (11.7 [2.3-58.9]), and M. genitalium infection (9.6 [3.1-29.9]) were associated

141 n men was also significantly associated with M. genitalium infection (OR = 2.97; 95% CI = 2.14 to 4.1

142 At baseline, 16.1% had prevalent M. genitalium infection and 40.4% had prevalent BV.

143 sequence variation in patients with chronic M. genitalium infection and to analyze the sequence stru

144 lyses assessing associations with persistent M. genitalium infection are needed.

145 as employed to estimate the odds of incident M. genitalium infection at follow-up visits among women

146 ymptoms had a significantly elevated risk of M. genitalium infection compared to that for asymptomati

147 Therefore, persistent M. genitalium infection could have important consequence

148 Here, we assessed the incidence of M. genitalium infection in patients attending a sexually

149 tively and quantitatively assess patterns of M. genitalium infection in women attending a sexually tr

150 Diagnosis of M. genitalium infection is recommended using a nucleic a

151 tis and N. gonorrhoeae infections, while the M. genitalium infection rate in males was significantly

152 Cure of M. genitalium infection resulted in ablation of all sign

153 eking care in the United States suggest that M. genitalium infection should be considered in young pe

154 ourse during menses were less likely to have M. genitalium infection than those who did not (odds rat

155 is and cervicitis had a higher prevalence of M. genitalium infection than women without those diagnos

156 male participants, the overall prevalence of M. genitalium infection was 10.3% and was significantly

157 Chronic M. genitalium infection was associated with increased se

158 The risk for M. genitalium infection was higher in black than in whit

159 rial STI (C. trachomatis, N. gonorrhoeae, or M. genitalium infection) was lower in the intervention a

160 e infection, and 0.66 [95% CI, .38-1.15] for M. genitalium infection).

161 ort female pig-tailed macaques as a model of M. genitalium infection, persistence, and immune evasion

162 were analyzed to describe the prevalence of M. genitalium infection, risk factors, and disease assoc

163 gle female pig-tailed macaque as a model for M. genitalium infection, we cervically inoculated eight

164 suitability of a pig-tailed macaque model of M. genitalium infection, we inoculated a pilot animal wi

165 s in sera from 101 adults with PCR-confirmed M. genitalium infection.

166 ggests that BV may enhance susceptibility to M. genitalium infection.

167 utinely screening any defined population for M. genitalium infection.

168 Of 244 evaluable M. genitalium infections (52 women, 68 heterosexual men,

169 There were 59 incident M. genitalium infections among 50 women, for an incidenc

170 estigations into the causal relationships of M. genitalium infections and clinical disease have been

171 ation of heretofore-unrecognized patterns of M. genitalium infections in clinical and experimental sa

172 ively whether screening for and treatment of M. genitalium infections in women and their sexual partn

173 Significant risk factors for M. genitalium infections were black race, younger age, n

174 Female M. genitalium infections were significantly more prevale

175 prove useful in management of some resistant M. genitalium infections, although it is not likely to a

176 distinguished asymptomatic from symptomatic M. genitalium infections.

177 prevent the emergence of ever more resistant M. genitalium infections.

178 tion, have been the treatments of choice for M. genitalium infections.

179 Antimicrobial-resistant M. genitalium is a significant problem and may require c

180 In this paper, we show that cytadherence in M. genitalium is affected by an unrelated protein known

181 Because M. genitalium is associated with both HIV acquisition an

182 Although M. genitalium is considered a leading cause of genitouri

183 M. genitalium is difficult to culture, and in the absenc

184 plasma hominis and the attachment protein of M. genitalium, is an integral membrane protein.

185 le region of MG192 was amplified by PCR from M. genitalium isolates obtained at various time points p

186 This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-t

187 have previously shown that a mutant form of M. genitalium lacking methionine sulfoxide reductase A (

188 -negative nongonococcal urethritis and other M. genitalium-linked infectious etiologies has been very

189 MgpB and MgpC, but was insufficient to clear M. genitalium lower tract infection.

190 Testing of high-risk symptomatic women for M. genitalium may be warranted.

191 Some studies suggest that M. genitalium may increase the risk of HIV acquisition.

192 ity of the third gene (MG192 or mgpC) of the M. genitalium MgPa adhesion operon, demonstrated that th

193 An M. genitalium mutant lacking the MG491 segment correspon

194 fold more likely to shed HIV-1 DNA than were M. genitalium-negative women (adjusted OR, 2.9 [95% conf

195 to arise from the introduction of diagnostic M. genitalium nucleic acid amplification testing includi

196 ns are needed to better define the impact of M. genitalium on women's reproductive health.

197 ates among subjects with monoinfections with M. genitalium or C. trachomatis compared to women with n

198 Women with high M. genitalium organism burdens (more than the median of

199 confidently predict the existence of viable M. genitalium organisms in cervical and vaginal samples.

200 hich at most time points the median ratio of M. genitalium organisms to host cells was </=10, indicat

201 nd sequence analysis of single-colony cloned M. genitalium organisms.

202 ion (STI), 35.9% exhibited sole detection of M. genitalium (P </= 0.0004 versus sole detection of oth

203 1.4% identity with the M. pneumoniae P30 and M. genitalium P32 cytadhesins, respectively.

204 with failure we sequenced key regions of the M. genitalium parC and gyrA genes for patients undergoin

205 -tailed macaque is a suitable model to study M. genitalium pathogenesis, antibody-mediated selection

206 ndicate that MsrA plays an important role in M. genitalium pathogenicity, possibly by protecting myco

207 In the upper tract, more than 90% of M. genitalium PCR-positive samples were from the uterus

208 Viable M. genitalium persisted in the lower genital tract for 8

209 Genetic diversity of M. genitalium populations was determined to infer whethe

210 , 16.8% of women and 15.1% of men were found M. genitalium positive.

211 stance was detected in 38% (385/1008) of the M. genitalium-positive patients, and the highest rate wa

212 upings support MRM reflex testing on primary M. genitalium-positive specimens.

213 assess macrolide antibiotic resistance among M. genitalium-positive subjects.

214 Among the M. genitalium-positive women, the relative sensitivities

215 Our results also suggest that M. genitalium possesses an active nucleotide excision re

216 Surveillance of M. genitalium prevalence and antimicrobial resistance pa

217 M. genitalium prevalence rates were 16.1% for females an

218 M. genitalium prevalence was 10.2% in females and 10.6%

219 Among 217 men, M. genitalium prevalence was 16.7% (95% confidence inter

220 ve such a set, we compared the 468 predicted M. genitalium protein sequences with the 1703 protein se

221 Herein, we identify 382 of the 482 M. genitalium protein-coding genes as essential, plus fi

222 Analytical sensitivity of the tests for M. genitalium ranged from 0.017 to 0.040 genome equivale

223 The specificity of the cobas assay for M. genitalium ranged from 96.0% to 99.8% across symptoma

224 We conclude that M. genitalium RecA has a primary role in mgpB/C-MgPar re

225 Positive M. genitalium results were confirmed by repeat testing o

226 treatment, and public health significance of M. genitalium reviewed at the meeting is described in de

227 Therefore, M. genitalium RNase R can precisely remove the 3'-traile

228 that the only exoribonuclease identified in M. genitalium, RNase R, is able to remove tRNA 3'-traile

229 pand these studies by examining the roles of M. genitalium ruvA and ruvB homologs.

230 d to improve understanding of key aspects of M. genitalium's natural history before it will be possib

231 ng for M. genitalium has been advocated, but M. genitalium's natural history is poorly-understood, ma

232 However, there is important uncertainty in M. genitalium's natural history parameters, leading to u

233 and behavioural studies to better understand M. genitalium's natural history, and then examined the e

234 Consideration of the cost-effectiveness of M. genitalium screening interventions may be warranted.

235 These data suggest that M. genitalium selectively regulates a limited number of

236 PDB sequences (274) match all of 106 M. genitalium sequences and some parts of another 85; th

237 ion, we show that the domains in the matched M. genitalium sequences come from 114 superfamilies and

238 These results show a high prevalence of M. genitalium single infections, a lower prevalence of c

239 nce variation occurred during the process of M. genitalium single-colony cloning.

240 M. genitalium-specific IgG, but not IgA, was detected in

241 M. genitalium-specific serum antibodies targeting the im

242 ts, MG192 sequences were more related within M. genitalium specimens from an individual patient than

243 italium virulence, we compared the wild-type M. genitalium strain (G37) with an msrA mutant (MS5) str

244 organism, we examined mgpB variation within M. genitalium strain G-37 and observed sequence heteroge

245 infection, we inoculated a pilot animal with M. genitalium strain G37 in the uterine cervix and in sa

246 specimens from two chimpanzees infected with M. genitalium strain G37.

247 rent mgpB variants within a single infecting M. genitalium strain, confirming that mgpB heterogeneity

248 192 gene is highly variable among and within M. genitalium strains in vitro and in vivo, and identifi

249 bacterial pathogens, genomic diversity among M. genitalium strains worldwide is unknown.

250 quences, as well as mgpB variability between M. genitalium strains, suggests that mgpB and MgPar sequ

251 These correlates differ from those found in M. genitalium studies conducted with FSW from West Afric

252 served except with superphysiologic loads of M. genitalium, suggesting that persistent infection occu

253 age in a 5-year period where all diagnostic M. genitalium testing in Denmark was centralized at the

254 establish the cost-effectiveness of routine M. genitalium testing in symptomatic patients and screen

255 health agencies should consider integrating M. genitalium testing into the management of persons wit

256 The aim of the study was to analyze the M. genitalium testing pattern and distribution of positi

257 dom use were more likely to be infected with M. genitalium than those who reported less frequent use

258 In M. genitalium, this transcription terminates at two clos

259 We conclude that the M. genitalium TMA and PCR assays are highly specific and

260 Incorporation of M. genitalium TMA into comprehensive testing programs wo

261 investigated the transcriptional response of M. genitalium to elevated temperatures and detected the

262 This study highlights the capacity of M. genitalium to elicit cervical inflammation and, consi

263 STIs and BV, there was a positive trend for M. genitalium to predict cervicitis (AOR, 3.18 [95% conf

264 Attachment of M. genitalium to the host cell's apical surface was obse

265 In addition, dissemination of M. genitalium to the knee tissues was observed as early

266 ch the sequences of the 467 gene products of M. genitalium to the sequences of the domains that form

267 In this study, we found that an M. genitalium transposon mutant that lacks expression of

268 diol and then inoculated intravaginally with M. genitalium type strain G37 or a contemporary Danish s

269 e than a single 1g dose at achieving cure of M. genitalium urethritis and importantly did not reduce

270 lide and new approaches for the treatment of M. genitalium urethritis are required.

271 obiological cure was determined for men with M. genitalium urethritis treated with azithromycin 1.5g

272 of this study was to investigate if and how M. genitalium uses a minimal genome to generate genetic

273 ed were tested for detection and quantity of M. genitalium using polymerase chain reaction analysis.

274 s (TV)/MG assay (cobas) for the detection of M. genitalium, using 22,150 urogenital specimens from bo

275 s into the mechanisms by which MsrA controls M. genitalium virulence, we compared the wild-type M. ge

276 The prevalence of M. genitalium was 12.9%.

277 M. genitalium was also less prevalent among FSW who had

278 prior urethritis, and chlamydial infection, M. genitalium was associated with a 6.5-fold increased r

279 unclear, and we sought to determine whether M. genitalium was capable of activating innate immune re

280 at replicates in Escherichia coli but not in M. genitalium was constructed to disrupt the cytadherenc

281 ase chain reaction (PCR) in 154 women (51%); M. genitalium was detected by qualitative PCR in 52 (17%

282 Among the women, M. genitalium was detected by the TMA and PCR assays in

283 M. genitalium was detected in 10.5% of stored specimens

284 Among the men, M. genitalium was detected in 24 urine specimens (6.8%)

285 M. genitalium was detected in 27 (22%) of 121 NGU case p

286 M. genitalium was detected in 282 (11.4%) patients.

287 M. genitalium was detected in 50 (7.0%) of 719 women.

288 M. genitalium was independently associated with detectio

289 Among 400 women studied, M. genitalium was independently significantly associated

290 tion-PCR showed that expression of MG_454 in M. genitalium was not elevated in response to oxidative

291 als inoculated in salpingeal pockets, viable M. genitalium was recovered for 2 weeks.

292 Using a novel quantitative PCR assay, M. genitalium was shown to replicate from 0 to 80 days p

293 e of 22 (41%; 95% CI, 20%-62%) patients with M. genitalium were infected with DLSTs possessing genoty

294 Three Mn-binding proteins (MnBPs) of M. genitalium were isolated by affinity chromatography.

295 and douching were positively associated with M. genitalium, whereas bacterial vaginosis and cunniling

296 iptional organization of the RuvAB system of M. genitalium, which is cotranscribed with two novel ope

297 t cells, MG_186 has the potential to provide M. genitalium, which possesses the smallest genome of an

298 of adherence, we examined the interaction of M. genitalium with a primary component of the mucosal ep

299 This study examined the associations of M. genitalium with selected sexually transmitted infecti

300 ecimens from 28 958 patients were tested for M. genitalium, with an increasing trend from 3858 per ye