ライフサイエンスコーパス: MLST

1 MLST analysis demonstrated the presence of 35 different

2 MLST analysis indicated that the C. jejuni isolates util

3 MLST analysis of 15 representative isolates from differe

4 MLST analysis revealed no lineage specific differences b

5 MLST and PFGE demonstrated a capsular switching from CPS

6 MLST and plasmid analysis shows that MCRPEC are diversel

7 MLST assigned 80% of CR-Kp isolates to the ST258-clone.

8 MLST data revealed that all isolates harboring the major

9 MLST data suggested that there was a large amount of gen

10 MLST demonstrated that the majority (87.5%; 7/8) of C. d

11 MLST findings revealed that sequence type 5 (ST5) was th

12 MLST identified 24 sequence types (STs), of which 19 wer

13 MLST is used to generate allelic profiles to characteriz

14 MLST is useful for investigating the epidemiology, genet

15 MLST of 42 6C isolates revealed 12 genotypes distributed

16 MLST resolved 64 B. multivorans sequence types.

17 MLST results suggest 6C strains arose from independent r

18 MLST revealed a 1 in 3198 nucleotide difference between

19 MLST showed five clusters related to serotype 6A, two cl

20 MLST studies of selective isolates from the four protein

21 MLST was less discriminatory than either MLVA or REA, ye

22 MLST was used to assess the genetic diversity of 192 GBS

23 MLST, DNA microarrays, and genome sequencing has allowed

24 cleotide polymorphism (SNP) loci, forming 25 MLST subtypes.

25 y sequenced travel related isolates, and 312 MLST profiles.

26 Using 24 PHAT probes, all 62 MLST CGs in the representative E. coli collection were d

27 2 E. coli isolates, representing at least 62 MLST CGs and diverse disease states, using a "library-on

28 From 7343 MLST-characterised isolates, we sequenced 600 C. jejuni

29 environmental strains that had the same AFLP/MLST genotypes.

30 tween short sequence strings (k-mers) and an MLST allele library.

31 species level by use of kmer comparisons and MLST.

32 a more powerful typing method than MLVA and MLST combined (D = 0.67).

33 The PFGE, MLVA, and MLST profiles were consistent with the predominate types

34 h genetic diversity was revealed by PFGE and MLST.

35 truly nontypeable by Quellung reaction, and MLST and the presence of psaA proved useful in distingui

36 (mainly the United States) by serotyping and MLST and to investigate associations between subtype and

37 study demonstrates the use of serotyping and MLST to differentiate pathogenic from commensal isolates

38 on common epidemiological surrogates such as MLST.

39 catenated locus sequences within and between MLST clusters confirmed the seven previously named Achro

40 By MLST, all isolates belonged to the international clone I

41 solates, belong to sequence type 8 (ST-8) by MLST and serotype HS:1,8, further indicating the clonali

42 effect of genetic background (as assessed by MLST) over and above capsule.

43 ention that were previously characterized by MLST and pulsed-field gel electrophoresis (PFGE).

44 . acnes isolates previously characterized by MLST and representing types IA1 (n=145), IA2 (n=20), IB

45 PS type IV and thus further characterized by MLST typing, pulsed-field gel electrophoresis (PFGE), an

46 A. baumannii clonal lineages (as defined by MLST) circulated during the study, three of which are gl

47 C. acnes clinical isolates was determined by MLST.

48 t this clade that cannot be distinguished by MLST.

49 ging to major epidemic lineages, followed by MLST analysis to categorize isolates from common lineage

50 ates of an outbreak strain as those found by MLST and PFGE.

51 tes were the predominant clone identified by MLST and MALDI-TOF, and CR-KP infection was associated w

52 Eighteen ST types were identified by MLST, among these ST types, forty-seven isolates belonge

53 riants (differing by a single nucleotide) by MLST and were therefore also classified as clonally rela

54 y positive and clustered with pneumococci by MLST (2 were bile soluble); 8 lacked psaA (5 ply positiv

55 cidence and molecular epidemiology of PPE by MLST in Utah children after the licensure of PCV-7.

56 uced results comparable to those produced by MLST for the identification of the major epidemic lineag

57 ccal sequence types previously recognized by MLST.

58 efore also classified as clonally related by MLST.

59 At least two major ecotypes, represented by MLST clades A and E, were proposed based on genetic, eco

60 existence of seven species was supported by MLST.

61 PHAT classification to the whole collection, MLST validation of the PHAT probe classification resulte

62 The most common MLST genotypes among the isolates were the dominant glob

63 variants were detected in each of the common MLST clonal complexes.

64 the housekeeping genes used by conventional MLST strategies.

65 ng its enhanced resolution over conventional MLST analysis.

66 The current MLST scheme uses sequences of 7 genes to generate an ST,

67 een 6C isolates shared one of four different MLST types with 6C-negative CS6As.

68 Moreover, this discriminatory MLST scheme retains the ability to identify epidemiologi

69 om six countries, with our newly established MLST scheme identified a total of 20 sequence types (STs

70 This facilitated an expanded MLST approach utilizing large numbers of loci for isolat

71 We propose that an extended MLST scheme with approximately 50 genes provides optimal

72 rcentages of isolates with complete extended MLST profiles ranged from 99.1% (50 genes) to 86.8% (1,4

73 lymorphism (SNP)-based method, (ii) extended MLST using different numbers of genes, (iii) determinati

74 From MLST, no more than 25% of cases could be linked to a pot

75 ines the sequence type of traditional 7-gene MLST with 100% accuracy in less than 10 seconds per isol

76 When compared with the nine gene MLST scheme developed at the University of Bath, UK, and

77 Genetic MLST clustering was confirmed by genomic sequence analys

78 trace back investigations, with core genome MLST (cgMLST) analysis as one of the most straightforwar

79 A core genome MLST (cgMLST) scheme defines a comprehensive set of thos

80 In our work, we used core genome MLST (cgMLST) to identify new outbreaks combined to core

81 Whole-genome MLST effectively distinguished between highly similar ou

82 Another clonal group (MLST ST1901) possessed 7 unique PBP2 patterns, and it sh

83 distinctions between strains with identical MLST profiles and showed a discriminatory power similar

84 st all (97%) of the R variants were found in MLST clonal complex 8 (CC8), while the H variant was bro

85 were MS4 and MS6, which matched with ST11 in MLST analysis.

86 gpi genes are not good candidates for use in MLST analysis and that a SBT-bla(OXA-51-like) gene schem

87 al to expand to more loci than those used in MLST and even to other bacterial species.

88 ied as separate STs by the Pasteur Institute MLST system, and an ST131 PCR method that targets the O2

89 All major MLST complexes include strains in both subfamily A and s

90 Using the scheme PFGE-MLVA-MLST-prn mutations-Prn deficiency, the 240 isolates comp

91 From this database, one or more MLST sequence types (STs) that comprised a PSGS genotype

92 In addition, a new MLST profile (ST1071) was observed in South Africa.

93 , although three novel spa types and a novel MLST (ST1518) were detected.

94 Here, we describe the development of a novel MLST system to assist with the investigation of an unusu

95 The data obtained by analysis of MLST and SBT-bla(OXA-51-like) genes were compared to the

96 ble to that of spa typing (D = 0.445) and of MLST (D = 0.417) in the first collection.

97 his study demonstrates that a combination of MLST and MLVA may prove useful for the investigation and

98 manner similar to major clonal complexes of MLST, indicating coevolution between the chromosomal bac

99 tes, one had a composite genotype profile of MLST ST 5-PFGE USA100-unknown spa type, which has been r

100 f discrimination similar in value to that of MLST (0.924 and 0.953 compared to 0.96), but discriminat

101 The utility of MLST in regard to cystic fibrosis (CF)-related infection

102 d using the asymmetric island model based on MLST data of human and animal/food isolates.

103 e investigated M. bovis population, based on MLST data.

104 mean ISS for each S. aureus clone (based on MLST) was compared with its DNA microarray-based genotyp

105 Based on MLST, we selected several B. anthracis, B. cereus, and B

106 pa type 539/t034 that were of ST398 based on MLST.

107 Forty-one MLST sequence types (STs) were observed.

108 (CCs) were assigned based on the spa and/or MLST results.

109 We performed MLST with 65 of the 334 surveillance isolates (61 S. dys

110 Using PFGE, MLST, and spa typing, three retail beef MRSA isolates we

111 led a limited diversity in surface proteins, MLST types, and DNA macrorestriction profiles for type I

112 corresponding to the Streptococcus pyogenes MLST scheme.

113 relatedness of the 46 strains recapitulated MLST types and provided greater interstrain differentiat

114 hromosome transfer of vancomycin resistance, MLST markers, and capsule genes as well.

115 f discrimination range from 0.972 (ribosomal MLST) to 0.999 (SNP based), and all values were higher t

116 d gel electrophoresis) patterns and the same MLST (multilocus sequence typing) sequence type (ST-1068

117 We also used the same MLST system to perform a retrospective analysis on isola

118 scribed a multilocus sequence typing scheme (MLST) for P. acnes based on seven housekeeping genes.

119 the first multilocus sequence typing scheme (MLST) for P. larvae, which largely confirms the previous

120 as sequence type 2109 (ST2109)-a rarely seen MLST sequence type.

121 Seven MLST types were identified, with the majority of the iso

122 ausing different invasive infections, shared MLST complexes and exhibited identical or closely relate

123 sent a coalescent method to jointly simulate MLST data and the clonal genealogy that gave rise to the

124 All the isolates belonged to a single MLST type, sequence type ST19.

125 isolates, respectively) represented a single MLST type.

126 he isolates recovered from horses, a smaller MLST and MLSA defined sub-population seems to be able to

127 ified a large and clonal lineage of strains (MLST ST9363) associated with elevated azithromycin minim

128 PCR was found to be more discriminatory than MLST/staphylococcal cassette chromosome mec (SCCmec) typ

129 We conclude that MLST and core genome SNP typing result in the same phylo

130 ions, and repertoires, and MDS revealed that MLST genotypes were strongly associated with particular

131 These observations show that MLST-derived genotype similarities between C. neoformans

132 The MLST loci used in this scheme were shown to be stable in

133 The MLST scheme uses genes with an appropriate clock speed a

134 The MLST-STs were more widely distributed among core genome

135 ory and demonstrated relationships among the MLST genetic lineages and REA genotypes that were previo

136 re appeared to be an association between the MLST types and protein expression profiles.

137 olates, while the second isolate carried the MLST ST 8-PFGE USA300-spa type t121 genotype, commonly i

138 However, the MLST data were not always in concordance with the PFGE d

139 Importantly, the MLST data suggest that B. burgdorferi originated in Euro

140 Despite the low level of diversity in the MLST loci, a neighbor net analysis revealed a variable r

141 phical segregation were both observed in the MLST subtypes.

142 ased on the relatedness of the isolates, the MLST data supported the hypothesis that infections in th

143 The molecular clock speed of the MLST genes was studied using 219 colonies isolated longi

144 eby providing the basis for extension of the MLST scheme, which is currently restricted to C. diphthe

145 We demonstrate the functionality of the MLST system in invasively and non-invasively collected s

146 Overall, the MLST and MLVA data were concordant with REA genotyping d

147 By adapting several primer sequences, the MLST genes in C. ulcerans were also amplified, thereby p

148 nomic sequence analysis, indicating that the MLST scheme developed in this study is representative of

149 cies and were resolved with reference to the MLST data.

150 d 24 sequence types (STs); 9 were new to the MLST database.

151 We validated the MLST scheme on B. burgdorferi specimens from North Ameri

152 ride different from that associated with the MLST lineage were considered to demonstrate capsular swi

153 on PFGE were largely in concordance with the MLST results, with a similar amount of genetic diversity

154 This MLST scheme was applied to 100 V. parahaemolyticus strai

155 This MLST system showed high intraspecies discriminatory powe

156 Furthermore, this MLST scheme was shown to be more discriminatory than bot

157 Application of this MLST scheme to more V. parahaemolyticus strains and by d

158 Using this MLST scheme, we analyzed 107 genetically diverse Achromo

159 According to MLST, all isolates belonged to sequence type 36.

160 s more closely related to VGIII according to MLST.

161 al setting and may provide an alternative to MLST for discriminating isolates.

162 ajor lineages (E1 and E2), both belonging to MLST sequence type 11 the major ST among serovar Enterit

163 ococcal Opa repertoire is strongly linked to MLST genotype irrespective of epidemiological sampling a

164 ates of Fusarium collected were subjected to MLST to identify the phylogenetic species and sequence t

165 This confirms that WGS is superior to MLST for evolutionary analyses and is more accurate than

166 cgMLST to those of other means (traditional MLST, pulsed-field gel electrophoresis [PFGE], and singl

167 dy was to compare the performance of the two MLST schemes and identify a new reference scheme capable

168 plexes, belongs to multilocus sequence type (MLST) 10, and is most closely related to strains isolate

169 moniae serotype 1, multilocus sequence type (MLST) 227.

170 palindromic-PCR), multilocus sequence type (MLST) analysis, and WGS on 148 Acinetobacter calcoacetic

171 correlated with a multilocus sequence type (MLST) clonal complex associated with specific PVL phage

172 ains with the same multilocus sequence type (MLST).

173 ferent serotypes, multilocus sequence types (MLST), and sites of clinical isolation.

174 ylogeny, identify multilocus sequence types (MLST), multiantigen sequence types (NG-MAST), and molecu

175 geny and identify multilocus sequence types (MLST), N. gonorrhoeae multiantigen sequence types (NG-MA

176 types and MCRPEC multi-locus sequence types (MLSTs) were more variable in Guangdong than in Zhejiang

177 nce between PFGE, multilocus sequence types (MLSTs), and rtxA gene sequencing results was also examin

178 s representing 24 multilocus sequence types (MLSTs), including human commensal and clinical isolates

179 3 spa types and 2 multilocus sequence types (MLSTs).

180 ntries presented multilocus sequence typing (MLST) alleles, sequence types and emm types (only 56% of

181 performed using multilocus sequence typing (MLST) alongside traditional phenotypic methods.

182 Multilocus sequence typing (MLST) analysis was performed on a subset of the strains.

183 anine sources by multilocus sequence typing (MLST) and examined their profile of putative adhesin-enc

184 ty inferred from multilocus sequence typing (MLST) and genome-wide SNP-based phylogenetic assays were

185 infections with Multilocus sequence typing (MLST) and Matrix-assisted laser desorption ionization-ti

186 Multilocus sequence typing (MLST) and multi-virulence-locus sequence typing (MVLST)

187 In this study, multilocus sequence typing (MLST) and multilocus variable-number tandem-repeat analy

188 arly typed using multilocus sequence typing (MLST) and multispacer sequence typing (MST).

189 characterized by multilocus sequence typing (MLST) and outer membrane protein gene sequencing.

190 genotyped using multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE).

191 were analyzed by multilocus sequence typing (MLST) and sequence analysis of virulence-associated gene

192 MSSA isolates by multilocus sequence typing (MLST) and spa typing in this study showed a genetic simi

193 solates included multilocus sequence typing (MLST) and staphylococcal protein A gene (spa) typing res

194 gene sequencing, multilocus sequence typing (MLST) and whole genome clustering of data from comparati

195 susceptibility, Multilocus Sequence Typing (MLST) and Whole Genome Sequencing (WGS) analysis.

196 pared to that of multilocus sequence typing (MLST) and whole-genome optical maps.

197 newly developed multilocus sequence typing (MLST) approach and elucidates the diversity, distributio

198 , we have used a multilocus sequence typing (MLST) approach employing the alleles of 7 genes (atpD, f

199 poB), applying a multilocus sequence typing (MLST) approach.

200 Serotyping and multilocus sequence typing (MLST) are primary methods to differentiate strains, but

201 yping, and rapid multilocus sequence typing (MLST) by electrospray ionization mass spectrometry of PC

202 ntly belonged to multilocus sequence typing (MLST) clonal complex (CC)-21.

203 roups similar to multilocus sequence typing (MLST) clonal groups (CGs) could be determined.

204 een CPS type and multilocus sequence typing (MLST) cluster, with the remarkable exception of the worl

205 Multilocus sequence typing (MLST) confirmed the strains belong to ST59, ST131, ST219

206 Although multilocus sequence typing (MLST) currently represents the gold standard for unambig

207 Multilocus sequence typing (MLST) data demonstrated that livestock associated clonal

208 ructure based on multilocus sequence typing (MLST) data derived from the WGS data showed that the rem

209 A multilocus sequence typing (MLST) database for V. parahaemolyticus was created in 20

210 Multilocus sequence typing (MLST) demonstrated 21 known and 2 novel sequence types (

211 repertoires and multilocus sequence typing (MLST) genotypes.

212 or studies using multilocus sequence typing (MLST) have found specific GBS clones (e.g., sequence typ

213 between WGS and multilocus sequence typing (MLST) identified major discrepancies for 17% of isolates

214 pacer region and multilocus sequence typing (MLST) indicated a predominant tetO-positive, doxycycline

215 Multilocus sequence typing (MLST) is a genetic typing tool designed to provide infor

216 Multi-locus sequence typing (MLST) is a widely used method of characterization of bac

217 Multilocus sequence typing (MLST) is the gold standard genotyping technique for many

218 ination in seven multilocus sequence typing (MLST) loci from 94 invasive, colonizing, and bovine stra

219 and 8 targeting multilocus sequence typing (MLST) loci were employed for each of 229 highly diverse

220 We performed multilocus sequence typing (MLST) on a limited sampling of 6C isolates with differen

221 We performed multilocus sequence typing (MLST) on all isolates and sequenced fragments of the luk

222 We formulated multilocus sequence typing (MLST) primers with six of the seven loci corresponding t

223 Recent multilocus sequence typing (MLST) refined the relatedness of B. cereus group members

224 enes agreed with multilocus sequence typing (MLST) results for typing of 49 of the 50 isolates; in ad

225 Multilocus sequence typing (MLST) revealed 24 sequence types (STs); 9 were new to th

226 resis (PFGE) and multilocus sequence typing (MLST) revealed a high level of concordance in the cluste

227 Multilocus sequence typing (MLST) revealed sequence type 5 (ST5) (n = 2), ST6 (n = 3

228 ore, developed a multilocus sequence typing (MLST) scheme for B. burgdorferi based on eight chromosom

229 A multilocus sequence typing (MLST) scheme for M. pneumoniae was developed based on th

230 k, we describe a multilocus sequence typing (MLST) scheme for V. parahaemolyticus based on the intern

231 this pathogen, a multilocus sequence typing (MLST) scheme was developed and applied to the characteri

232 robustness of a multilocus sequence typing (MLST) scheme, based on conserved regions of seven housek

233 , we introduce a multilocus sequence typing (MLST) scheme, comprised of seven single-copy housekeepin

234 elop an expanded multilocus sequence typing (MLST) scheme.

235 ed a genus-level multilocus sequence typing (MLST) scheme.

236 Two multilocus sequence typing (MLST) schemes have been developed for M. bovis, with one

237 s and subsequent multilocus sequence typing (MLST) separated a subset of 77 isolates into 24 sequence

238 Multi locus sequence typing (MLST) suggests presence of genetic homogeneity across wS

239 first extensive multilocus sequence typing (MLST) survey of plumbing drain-associated Fusarium isola

240 1 by the Achtman multilocus sequence typing (MLST) system and a screening PCR assay that targets ST13

241 esis (PFGE), and multilocus sequence typing (MLST) to characterize them and identify trends in DNA cl

242 Here, we used multilocus sequence typing (MLST) to compare the molecular genotypes of strains of C

243 Multilocus sequence typing (MLST) together with macrorestriction analysis of genomic

244 Multilocus sequence typing (MLST) was able to accurately predict the serovars for 42

245 Nested multilocus sequence typing (MLST) was employed for case specimen extracts.

246 Multilocus sequence typing (MLST) was performed on 107 B. multivorans isolates to pr

247 esis (PFGE) and multi-locus sequence typing (MLST) were performed to assess clonality.

248 esis (PFGE), and multilocus sequence typing (MLST) were performed.

249 CR (rep-PCR) and multilocus sequence typing (MLST) were performed.

250 and psaA genes, multilocus sequence typing (MLST), 16S rRNA gene sequencing, and pyrosequencing.

251 e type (ST) 8 by multilocus sequence typing (MLST), all of which are characteristics commonly attribu

252 phoresis (PFGE), multilocus sequence typing (MLST), and clustered regularly interspaced short palindr

253 electrophoresis, multilocus sequence typing (MLST), and molecular capsule typing (C-patterns and wzi

254 analysis (MLVA), multilocus sequence typing (MLST), and pertactin gene (prn) mutational analysis.

255 analysis (REA), multilocus sequence typing (MLST), and pulsed-field gel electrophoresis (PFGE).

256 phoresis (PFGE), multilocus sequence typing (MLST), and serotyping.

257 A (spa) typing, multilocus sequence typing (MLST), and staphylococcal cassette chromosome mec (SCCme

258 A (spa) typing, multilocus sequence typing (MLST), and staphylococcal cassette chromosome mec elemen

259 phoresis (PFGE), multilocus sequence typing (MLST), and staphylococcal protein A (spa) typing.

260 olates underwent multilocus sequence typing (MLST), as well as assays for the Panton-Valentine leukoc

261 roaches, such as multilocus sequence typing (MLST), by substantially increasing the number of loci ex

262 rable to that of multilocus sequence typing (MLST), is applicable to mixtures, and is highly automate

263 T), analogous to multilocus sequence typing (MLST), is the current "gold standard" typing method for

264 nalyzed by using multilocus sequence typing (MLST), plasmid profiling, hybridization to a pan-Salmone

265 PFGE) and 7-gene multilocus sequence typing (MLST), provided limited resolution to adequately identif

266 t Britain, using multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and arra

267 ibility testing, multilocus sequence typing (MLST), spa typing, SCCmec typing, and pulsed-field gel e

268 rmed as ST398 by multilocus sequence typing (MLST), which prompted retrospective analysis of all MRSA

269 ere subjected to multilocus sequence typing (MLST).

270 were subtyped by multilocus sequence typing (MLST).

271 PCR (repPCR) and multilocus sequence typing (MLST).

272 gerprinting, and multilocus sequence typing (MLST).

273 action (PCR) and multilocus sequence typing (MLST).

274 ere genotyped by multilocus sequence typing (MLST).

275 ere evaluated by multilocus sequence typing (MLST).

276 ages defined by multi-locus sequence typing (MLST).

277 s then underwent multilocus sequence typing (MLST).

278 esis (PFGE), and multilocus sequence typing (MLST).

279 ion mapping, and multilocus sequence typing (MLST).

280 idate genes for multi-locus sequence typing (MLST).

281 enes analyzed in multilocus sequence typing (MLST).

282 hisms (AFLP) and multilocus sequence typing (MLST).

283 n sequencing for multilocus sequence typing (MLST).

284 ined by standard multilocus sequence typing (MLST).

285 characterized by multilocus sequence typing (MLST).

286 y congruent with multilocus sequence typing (MLST).

287 ssigned by using multilocus sequence typing (MLST).

288 d, and ribosomal multilocus sequence typing (MLST, eMLST, and rMLST); antigen gene sequence typing (A

289 Multi locus sequencing typing (MLST) analysis identified two strains of tsetse-associat

290 rigins (based on multilocus sequence typing [MLST] analysis), and did not arise recently.

291 s (determined by multilocus sequence typing [MLST]) and 79 pulsed-field gel electrophoresis types.

292 ned population of ~600,000 persons underwent MLST.

293 ST5, ST8, ST9, and ST30) were detected using MLST; the majority of MRSA isolates belonged to ST8, fol

294 KPC-KP isolates were MLST-typed.

295 ily due to serotypes 1, 3, 19A, and 7F, with MLST demonstrating sequence types (ST) that were commonl

296 meningitidis strains are characterized with MLST as specific sequence types (ST) and clonal complexe

297 Compared with MLST, the assay has 100% sensitivity and 99.5% specifici

298 G45D mtrR mutations (p=0.046), but not with MLST or NG-MAST molecular types.

299 G45D mtrR mutations (P = .046) but not with MLST or NG-MAST types.

300 SERS typing correlated well with MLST indicating that it has high sensitivity and selecti